pycom11g19490

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0012344
Physical Location & Seq
Reverse (-)
22027785 .. 22029194
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1284 bp
ATGGCTACTCGAACACATAGACGCTGGTTCGGTTCTCGGGCCCGAAACAACAATCTCTGCTCACTTCTCCAAAACCCTTGCTTTCTTCCTCAAACCCTAAAAATGGTGACTACTCAGGCCGCTTCCAAACGACCCATTTGTCCATCGTGCTCCAAACCAGCTCGGCTCTGCCTCTGCTCTCGGATTCAGAACCCGGGGCTCCATAACTCTTTCGTTATCCGGCCGCTGGACTCGGTTTCTCAAATGGGTTCGGACCGGAATGGTTTGAAAAGTTTAGGTTTTGATGAAGTTGTTGAAAGTAGAGAGACCCAGAAGCTGGATTTTGAGTATTGTGATGGGTTCAGCCCAGGGGAAGAAAATGTGGGAAAGTTGGGATTTGGGTTATCTGCAAATTTGGCAGAAGAGAAAAACAATAGTAGTTTGATTGATTGTGAGAAATGCAATTTTGGTAGTGATTTAGGTGATATGAGTAGTTCTCGAAACCAATTAGGGGATGTAGATTTTGTGGAGAAAGAACATGAATTCAGTAGTGAAGATTTCACTGATGGTATACACGTAGATGGGCAATGCAATGTCGATGAGGTTTCTGCTTCTACTGCGTTGGATGCATTATCCGGGGATTCTGATGTAGGTTTTGATGCGGTTGAAGGTATCGAAAGGGGTAATGGTGATCCTGTTATAACTGCTACCATTGGTAAGCATGGCGTCATTAGCTCACTTTCGCATAATTGGATGCCACAAACCGGTTGCAAGGATCTGAAATTTGATACAATTTTAGACATAGCAGATGCGCGTGGTGCACTATCAGAGGGGTTTGTTGTACGGAAGTTGCAAAAGCAGCCGTTGAAGGGAAGCATGGAGTTGGATGAGTATGTGGAGTTTGAGGTAGAGGTTCCTCCTGGATCTGTACTCTTATTTCCATCTGAAGAAGCAGTCAGTGTCGGGGACCTTGAGGCGATGGATATTAAGGTGAAGAATCTGATTGTTTTGGATGGAACATGGTCAAAGGCAAAGAGAATGTATGGGGAAAACCCCTGGTTGAAACTCTTGCCGCACTTGAAGTTGAATGTGGACAAGATGAGCTTGTACAGTGAAGTGAGGCTTCAGCCGAAGCCGGGATTTCTCTCCACTATTGAGAGCATTGTGTATGCACTTAAAGCAGTAGGGGACAGCCCTGGGGCGCTGGCTGAGGGGCTGGACAACCTCTTGGATGTTTTCAGGTCTATGGTCGAGGACCAGATACGATGCAAAGAAGAGAGATTAAGCAATGTCTCGCCGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

428

Amino Acids

46.79

Weight (kDa)

4.89

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 299 - 413 3.3e-26 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 680
AasI GACNNNNNNGTC 1 cut(s) 138
AccB7I CCANNNNNTGG 1 cut(s) 316
AccI GTMKAC 1 cut(s) 550
AccII CGCG 1 cut(s) 793
AciI CCGC 4 cut(s) 120, 224, 641, 1052
AclWI GGATC 3 cut(s) 665, 762, 910
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 3 cut(s) 391, 521, 761
AcuI CTGAAG 2 cut(s) 945, 1088
AcyI GRCGYC 1 cut(s) 705
AfaI GTAC 3 cut(s) 822, 909, 1088
AfiI CCNNNNNNNGG 7 cut(s) 103, 226, 316, 490, 743, 848, 1115
AflIII ACRYGT 1 cut(s) 553
AgeI ACCGGT 1 cut(s) 743
AgsI TTSAA 7 cut(s) 268, 296, 647, 847, 1042, 1060, 1066
AjnI CCWGG 4 cut(s) 346, 898, 1034, 1174
AluBI AGCT 4 cut(s) 161, 316, 714, 1083
AluI AGCT 4 cut(s) 161, 316, 714, 1083
Alw21I GWGCWC 2 cut(s) 152, 802
Alw26I GTCTC 2 cut(s) 299, 1276
Alw44I GTGCAC 1 cut(s) 798
AlwI GGATC 3 cut(s) 665, 762, 910
AlwNI CAGNNNCTG 1 cut(s) 316
Ama87I CYCGRG 2 cut(s) 36, 193
AoxI GGCC 3 cut(s) 39, 117, 221
ApaI GGGCCC 1 cut(s) 43
ApaLI GTGCAC 1 cut(s) 798
ApeKI GCWGC 1 cut(s) 838
ApoI RAATTY 3 cut(s) 391, 521, 761
AsiGI ACCGGT 1 cut(s) 743
AspLEI GCGC 2 cut(s) 793, 1183
AspS9I GGNCC 5 cut(s) 39, 40, 253, 946, 1234
AsuC2I CCSGG 5 cut(s) 194, 195, 616, 1116, 1278
AsuHPI GGTGA 4 cut(s) 118, 473, 680, 982
AvaI CYCGRG 2 cut(s) 36, 193
AvaII GGWCC 3 cut(s) 253, 946, 1234
BaeGI GKGCMC 2 cut(s) 43, 802
BanII GRGCYC 2 cut(s) 43, 201
Bbv12I GWGCWC 2 cut(s) 152, 802
BbvCI CCTCAGC 1 cut(s) 1188
BbvI GCAGC 1 cut(s) 850
BccI CCATC 7 cut(s) 151, 329, 539, 554, 928, 952, 986
BceAI ACGGC 1 cut(s) 826
BciT130I CCWGG 4 cut(s) 348, 900, 1036, 1176
BcnI CCSGG 5 cut(s) 194, 195, 616, 1116, 1278
BcoDI GTCTC 2 cut(s) 299, 1276
BfoI RGCGCY 1 cut(s) 1184
BisI GCNGC 4 cut(s) 120, 224, 839, 1052
BlsI GCNGC 4 cut(s) 121, 225, 840, 1053
Bme1390I CCNGG 9 cut(s) 194, 195, 348, 616, 900, 1036, 1116, 1176, 1278
Bme18I GGWCC 3 cut(s) 253, 946, 1234
BmeT110I CYCGRG 2 cut(s) 36, 193
BmgT120I GGNCC 5 cut(s) 39, 40, 253, 946, 1234
BmiI GGNNCC 4 cut(s) 41, 200, 894, 947
BmrFI CCNGG 9 cut(s) 194, 195, 348, 616, 900, 1036, 1116, 1176, 1278
BmsI GCATC 5 cut(s) 595, 628, 723, 778, 1235
BplI GAGNNNNNCTC 2 cut(s) 460, 492
Bpu10I CCTNAGC 1 cut(s) 1188
BpuEI CTTGAG 1 cut(s) 971
BpuMI CCSGG 5 cut(s) 194, 195, 616, 1116, 1278
BsaAI YACGTR 1 cut(s) 556
BsaHI GRCGYC 1 cut(s) 705
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 9 cut(s) 193, 194, 346, 347, 615, 1034, 1174, 1175, 1277
BsaWI WCCGGW 2 cut(s) 255, 743
Bsc4I CCNNNNNNNGG 7 cut(s) 103, 226, 316, 490, 743, 848, 1115
Bse118I RCCGGY 1 cut(s) 743
Bse3DI GCAATG 3 cut(s) 572, 577, 1273
BseBI CCWGG 4 cut(s) 348, 900, 1036, 1176
BseDI CCNNGG 9 cut(s) 193, 194, 346, 347, 615, 1034, 1174, 1175, 1277
BseGI GGATG 6 cut(s) 499, 610, 738, 871, 997, 1216
BseLI CCNNNNNNNGG 7 cut(s) 103, 226, 316, 490, 743, 848, 1115
BseMI GCAATG 3 cut(s) 572, 577, 1273
BseMII CTCAG 2 cut(s) 128, 1179
BseSI GKGCMC 2 cut(s) 43, 802
BseX3I CGGCCG 1 cut(s) 221
BseXI GCAGC 1 cut(s) 850
Bsh1236I CGCG 1 cut(s) 793
Bsh1285I CGRYCG 1 cut(s) 224
BshFI GGCC 3 cut(s) 41, 119, 223
BshTI ACCGGT 1 cut(s) 743
BsiEI CGRYCG 1 cut(s) 224
BsiHKAI GWGCWC 2 cut(s) 152, 802
BsiHKCI CYCGRG 2 cut(s) 36, 193
BsiSI CCGG 7 cut(s) 194, 220, 256, 615, 744, 1115, 1277
BslFI GGGAC 2 cut(s) 959, 1181
BslI CCNNNNNNNGG 7 cut(s) 103, 226, 316, 490, 743, 848, 1115
BsmAI GTCTC 2 cut(s) 299, 1276
BsmFI GGGAC 2 cut(s) 959, 1181
BsnI GGCC 3 cut(s) 41, 119, 223
Bso31I GGTCTC 1 cut(s) 299
BsoBI CYCGRG 2 cut(s) 36, 193
Bsp120I GGGCCC 1 cut(s) 39
Bsp1286I GDGCHC 4 cut(s) 43, 152, 201, 802
Bsp1407I TGTACA 1 cut(s) 1086
Bsp143I GATC 3 cut(s) 670, 754, 902
BspACI CCGC 4 cut(s) 120, 224, 641, 1052
BspANI GGCC 3 cut(s) 41, 119, 223
BspCNI CTCAG 2 cut(s) 127, 1180
BspFNI CGCG 1 cut(s) 793
BspLI GGNNCC 4 cut(s) 41, 200, 894, 947
BspPI GGATC 3 cut(s) 665, 762, 910
BspTNI GGTCTC 1 cut(s) 299
BsrDI GCAATG 3 cut(s) 572, 577, 1273
BsrFI RCCGGY 1 cut(s) 743
BsrGI TGTACA 1 cut(s) 1086
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 9 cut(s) 193, 194, 346, 347, 615, 1034, 1174, 1175, 1277
BssMI GATC 3 cut(s) 670, 754, 902
BssNAI GTATAC 1 cut(s) 551
BssNI GRCGYC 1 cut(s) 705
Bst1107I GTATAC 1 cut(s) 551
Bst2UI CCWGG 4 cut(s) 348, 900, 1036, 1176
Bst4CI ACNGT 1 cut(s) 1091
Bst6I CTCTTC 2 cut(s) 396, 1248
BstACI GRCGYC 1 cut(s) 705
BstAUI TGTACA 1 cut(s) 1086
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 1185
BstDEI CTNAG 2 cut(s) 114, 1188
BstF5I GGATG 6 cut(s) 499, 610, 738, 871, 997, 1216
BstFNI CGCG 1 cut(s) 793
BstH2I RGCGCY 1 cut(s) 1184
BstHHI GCGC 2 cut(s) 793, 1183
BstKTI GATC 3 cut(s) 673, 757, 905
BstMAI GTCTC 2 cut(s) 299, 1276
BstMBI GATC 3 cut(s) 670, 754, 902
BstMCI CGRYCG 1 cut(s) 224
BstMWI GCNNNNNNNGC 7 cut(s) 395, 596, 605, 711, 797, 838, 1157
BstNI CCWGG 4 cut(s) 348, 900, 1036, 1176
BstSCI CCNGG 9 cut(s) 192, 193, 346, 614, 898, 1034, 1114, 1174, 1276
BstSLI GKGCMC 2 cut(s) 43, 802
BstUI CGCG 1 cut(s) 793
BstV1I GCAGC 1 cut(s) 850
BstX2I RGATCY 2 cut(s) 754, 902
BstYI RGATCY 2 cut(s) 754, 902
BstZ17I GTATAC 1 cut(s) 551
BstZI CGGCCG 1 cut(s) 221
BsuRI GGCC 3 cut(s) 41, 119, 223
BtgZI GCGATG 1 cut(s) 971
BtsCI GGATG 6 cut(s) 499, 610, 738, 871, 997, 1216
BtsIMutI CAGTG 3 cut(s) 540, 943, 1096
Cac8I GCNNGC 1 cut(s) 1185
CaiI CAGNNNCTG 1 cut(s) 316
CfoI GCGC 2 cut(s) 793, 1183
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 5 cut(s) 39, 40, 253, 946, 1234
Cfr9I CCCGGG 1 cut(s) 193
CpoI CGGWCCG 1 cut(s) 253
CseI GACGC 2 cut(s) 30, 694
Csp6I GTAC 3 cut(s) 821, 908, 1087
CspAI ACCGGT 1 cut(s) 743
CspI CGGWCCG 1 cut(s) 253
CviAII CATG 4 cut(s) 518, 701, 856, 999
CviQI GTAC 3 cut(s) 821, 908, 1087
DdeI CTNAG 2 cut(s) 114, 1188
DpnI GATC 3 cut(s) 672, 756, 904
DpnII GATC 3 cut(s) 670, 754, 902
DrdI GACNNNNNNGTC 1 cut(s) 138
DseDI GACNNNNNNGTC 1 cut(s) 138
EaeI YGGCCR 1 cut(s) 221
EagI CGGCCG 1 cut(s) 221
Eam1104I CTCTTC 2 cut(s) 396, 1248
EarI CTCTTC 2 cut(s) 396, 1248
EclXI CGGCCG 1 cut(s) 221
Eco24I GRGCYC 2 cut(s) 43, 201
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 3 cut(s) 253, 946, 1234
Eco52I CGGCCG 1 cut(s) 221
Eco57I CTGAAG 2 cut(s) 945, 1088
Eco88I CYCGRG 2 cut(s) 36, 193
EcoO109I RGGNCCY 1 cut(s) 946
EcoRI GAATTC 1 cut(s) 521
EcoRII CCWGG 4 cut(s) 346, 898, 1034, 1174
EcoT22I ATGCAT 1 cut(s) 610
EcoT38I GRGCYC 2 cut(s) 43, 201
FaeI CATG 4 cut(s) 521, 704, 859, 1002
FalI AAGNNNNNCTT 4 cut(s) 1067, 1099, 1086, 1118
FaqI GGGAC 2 cut(s) 959, 1181
FatI CATG 4 cut(s) 517, 700, 855, 998
FblI GTMKAC 1 cut(s) 550
Fnu4HI GCNGC 4 cut(s) 120, 224, 839, 1052
FokI GGATG 6 cut(s) 506, 617, 745, 878, 1004, 1223
FriOI GRGCYC 2 cut(s) 43, 201
Fsp4HI GCNGC 4 cut(s) 120, 224, 839, 1052
GlaI GCGC 2 cut(s) 792, 1182
GluI GCNGC 4 cut(s) 120, 224, 839, 1052
HaeII RGCGCY 1 cut(s) 1184
HaeIII GGCC 3 cut(s) 41, 119, 223
HapII CCGG 7 cut(s) 194, 220, 256, 615, 744, 1115, 1277
HgaI GACGC 2 cut(s) 30, 694
HhaI GCGC 2 cut(s) 793, 1183
Hin1I GRCGYC 1 cut(s) 705
Hin1II CATG 4 cut(s) 521, 704, 859, 1002
Hin6I GCGC 2 cut(s) 791, 1181
HinP1I GCGC 2 cut(s) 791, 1181
HinfI GANTC 4 cut(s) 184, 230, 620, 976
HpaII CCGG 7 cut(s) 194, 220, 256, 615, 744, 1115, 1277
HphI GGTGA 4 cut(s) 118, 473, 680, 982
Hpy166II GTNNAC 3 cut(s) 551, 800, 1072
Hpy188I TCNGA 8 cut(s) 183, 189, 253, 625, 759, 808, 925, 981
Hpy188III TCNNGA 1 cut(s) 477
Hpy8I GTNNAC 3 cut(s) 551, 800, 1072
HpyAV CCTTC 2 cut(s) 641, 841
HpyCH4III ACNGT 1 cut(s) 1091
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 9 cut(s) 389, 441, 570, 608, 750, 800, 832, 1151, 1248
HpyF10VI GCNNNNNNNGC 7 cut(s) 395, 596, 605, 711, 797, 838, 1157
HpyF3I CTNAG 2 cut(s) 114, 1188
HpySE526I ACGT 1 cut(s) 555
Hsp92I GRCGYC 1 cut(s) 705
Hsp92II CATG 4 cut(s) 521, 704, 859, 1002
HspAI GCGC 2 cut(s) 791, 1181
Kzo9I GATC 3 cut(s) 670, 754, 902
LmnI GCTCC 2 cut(s) 155, 204
Lsp1109I GCAGC 1 cut(s) 850
LweI GCATC 5 cut(s) 595, 628, 723, 778, 1235
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 1 cut(s) 106
MalI GATC 3 cut(s) 672, 756, 904
MboI GATC 3 cut(s) 670, 754, 902
MboII GAAGA 7 cut(s) 77, 365, 413, 545, 938, 985, 1265
MflI RGATCY 2 cut(s) 754, 902
MhlI GDGCHC 4 cut(s) 43, 152, 201, 802
MluCI AATT 7 cut(s) 391, 442, 485, 521, 727, 761, 771
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 2 cut(s) 582, 843
Mph1103I ATGCAT 1 cut(s) 610
MseI TTAA 3 cut(s) 966, 1155, 1262
MslI CAYNNNNRTG 1 cut(s) 558
MspA1I CMGCKG 1 cut(s) 226
MspI CCGG 7 cut(s) 194, 220, 256, 615, 744, 1115, 1277
MspR9I CCNGG 9 cut(s) 194, 195, 348, 616, 900, 1036, 1116, 1176, 1278
MvaI CCWGG 4 cut(s) 348, 900, 1036, 1176
MvnI CGCG 1 cut(s) 793
MwoI GCNNNNNNNGC 7 cut(s) 395, 596, 605, 711, 797, 838, 1157
NciI CCSGG 5 cut(s) 194, 195, 616, 1116, 1278
NdeII GATC 3 cut(s) 670, 754, 902
NlaIII CATG 4 cut(s) 521, 704, 859, 1002
NlaIV GGNNCC 4 cut(s) 41, 200, 894, 947
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 106
NsiI ATGCAT 1 cut(s) 610
PasI CCCWGGG 2 cut(s) 347, 1175
PfeI GAWTC 3 cut(s) 184, 620, 976
PflMI CCANNNNNTGG 1 cut(s) 316
PfoI TCCNGGA 1 cut(s) 898
PinAI ACCGGT 1 cut(s) 743
PkrI GCNGC 4 cut(s) 121, 225, 840, 1053
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Ppu21I YACGTR 1 cut(s) 556
PpuMI RGGWCCY 1 cut(s) 946
PsiI TTATAA 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 946
Psp6I CCWGG 4 cut(s) 346, 898, 1034, 1174
PspGI CCWGG 4 cut(s) 346, 898, 1034, 1174
PspN4I GGNNCC 4 cut(s) 41, 200, 894, 947
PspOMI GGGCCC 1 cut(s) 39
PspPI GGNCC 5 cut(s) 39, 40, 253, 946, 1234
PspPPI RGGWCCY 1 cut(s) 946
PstNI CAGNNNCTG 1 cut(s) 316
PsuI RGATCY 2 cut(s) 754, 902
RsaI GTAC 3 cut(s) 822, 909, 1088
RsaNI GTAC 3 cut(s) 821, 908, 1087
RseI CAYNNNNRTG 1 cut(s) 558
Rsr2I CGGWCCG 1 cut(s) 253
RsrII CGGWCCG 1 cut(s) 253
SaqAI TTAA 3 cut(s) 966, 1155, 1262
SatI GCNGC 4 cut(s) 120, 224, 839, 1052
Sau3AI GATC 3 cut(s) 670, 754, 902
Sau96I GGNCC 5 cut(s) 39, 40, 253, 946, 1234
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 9 cut(s) 194, 195, 348, 616, 900, 1036, 1116, 1176, 1278
SduI GDGCHC 4 cut(s) 43, 152, 201, 802
SfaNI GCATC 5 cut(s) 595, 628, 723, 778, 1235
SinI GGWCC 3 cut(s) 253, 946, 1234
SmaI CCCGGG 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 950
SmoI CTYRAG 1 cut(s) 950
Sse9I AATT 7 cut(s) 391, 442, 485, 521, 727, 761, 771
SsiI CCGC 4 cut(s) 120, 224, 641, 1052
StyD4I CCNGG 9 cut(s) 192, 193, 346, 614, 898, 1034, 1114, 1174, 1276
TaaI ACNGT 1 cut(s) 1091
TaiI ACGT 1 cut(s) 558
TaqI TCGA 5 cut(s) 10, 478, 576, 654, 1230
TasI AATT 7 cut(s) 391, 442, 485, 521, 727, 761, 771
TatI WGTACW 2 cut(s) 907, 1086
TauI GCSGC 3 cut(s) 122, 226, 1054
TfiI GAWTC 3 cut(s) 184, 620, 976
Tru1I TTAA 3 cut(s) 966, 1155, 1262
Tru9I TTAA 3 cut(s) 966, 1155, 1262
TscAI CASTG 3 cut(s) 547, 943, 1096
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 1 cut(s) 838
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 2 cut(s) 300, 534
TspGWI ACGGA 1 cut(s) 838
TspMI CCCGGG 1 cut(s) 193
TspRI CASTG 3 cut(s) 547, 943, 1096
Van91I CCANNNNNTGG 1 cut(s) 316
VneI GTGCAC 1 cut(s) 798
VpaK11BI GGWCC 3 cut(s) 253, 946, 1234
XapI RAATTY 3 cut(s) 391, 521, 761
XmaI CCCGGG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 550
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.