pycom11g24010

sodium metabolite cotransporter BASS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006339
Physical Location & Seq
Forward (+)
26979880 .. 26980766
887 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 510 bp
ATGACCCCATTTCTCACAGCTAAACTTACCGGCCAATATGTCGCAGTAGATGCAGCTGGGCTCTTATTCTCAACACTGCAGGTTGTGCTTCTCCCTGTTTTGGCGGGTGCATTTCTGAATCAGTATTTCCAAGGCCTGGTTAAATTCATCTCCCCCGTGATGCCACCCATTGCTGTAGCAACTGTAGGTGTTCTTTGTGGGAATGCAATTGCCCAGAGTTCATCTGCAATCCTTGCGTCTGGTAAACAAGTGGTGCTTTCTTCCGCTCTTCTTCATGCAAGTGGATTTTTCTTTGGATACATCTTTTCAAGGATGCTTGGGCTTGATGTCACATCATCAAGGACTATCTCCATCGAGGTTGGCATGCAGAATTCGATGCTCGGAATTGTTCTAGCCGGTCAGCACTTCGGAAACCCTCTAACTGCAGTACCATGTGCTGTTTCCAGTGTTTGTCATTCAATCTTGGGGAGTGCCTTGGCAGGATTCTGGAGACAGACTGTGCCAACCTAG

Protein Analysis

170

Amino Acids

17.52

Weight (kDa)

8.99

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBF PF01758 1 - 77 1.5e-06 Sodium Bile acid symporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 70
AccB7I CCANNNNNTGG 1 cut(s) 136
AccBSI CCGCTC 1 cut(s) 266
AciI CCGC 2 cut(s) 104, 264
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 2 cut(s) 143, 370
AfaI GTAC 1 cut(s) 429
AfiI CCNNNNNNNGG 2 cut(s) 100, 136
AgsI TTSAA 2 cut(s) 309, 459
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 2 cut(s) 20, 56
AluI AGCT 2 cut(s) 20, 56
Alw26I GTCTC 1 cut(s) 484
AoxI GGCC 2 cut(s) 31, 133
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 2 cut(s) 143, 370
BaeI ACNNNNGTAYC 2 cut(s) 411, 444
BanII GRGCYC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 65
BccI CCATC 1 cut(s) 359
BciT130I CCWGG 1 cut(s) 137
BciVI GTATCC 1 cut(s) 290
BcoDI GTCTC 1 cut(s) 484
BfaI CTAG 2 cut(s) 392, 508
BfmI CTRYAG 4 cut(s) 77, 174, 183, 423
BfuAI ACCTGC 1 cut(s) 70
BfuI GTATCC 1 cut(s) 290
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
Bme1390I CCNGG 1 cut(s) 137
BmrFI CCNGG 1 cut(s) 137
BmsI GCATC 4 cut(s) 40, 150, 303, 366
BpmI CTGGAG 1 cut(s) 508
BsaJI CCNNGG 2 cut(s) 130, 474
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 136
Bse118I RCCGGY 2 cut(s) 29, 395
Bse1I ACTGG 1 cut(s) 444
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 130, 474
BseGI GGATG 1 cut(s) 318
BseLI CCNNNNNNNGG 2 cut(s) 100, 136
BseMI GCAATG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 444
BseXI GCAGC 1 cut(s) 65
BseYI CCCAGC 1 cut(s) 56
BshFI GGCC 2 cut(s) 33, 135
BsiSI CCGG 2 cut(s) 30, 396
BslI CCNNNNNNNGG 2 cut(s) 100, 136
BsmAI GTCTC 1 cut(s) 484
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 2 cut(s) 33, 135
Bsp1286I GDGCHC 1 cut(s) 63
BspACI CCGC 2 cut(s) 104, 264
BspANI GGCC 2 cut(s) 33, 135
BspMAI CTGCAG 2 cut(s) 81, 427
BspMI ACCTGC 1 cut(s) 70
BspQI GCTCTTC 1 cut(s) 273
BsrBI CCGCTC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 2 cut(s) 29, 395
BsrI ACTGG 1 cut(s) 444
BssAI RCCGGY 2 cut(s) 29, 395
BssECI CCNNGG 2 cut(s) 130, 474
BssT1I CCWWGG 2 cut(s) 130, 474
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 2 cut(s) 184, 499
Bst6I CTCTTC 1 cut(s) 273
BstAPI GCANNNNNTGC 3 cut(s) 50, 85, 233
BstC8I GCNNGC 1 cut(s) 365
BstF5I GGATG 1 cut(s) 318
BstMAI GTCTC 1 cut(s) 484
BstMWI GCNNNNNNNGC 3 cut(s) 50, 85, 233
BstNI CCWGG 1 cut(s) 137
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 4 cut(s) 77, 174, 183, 423
BstV1I GCAGC 1 cut(s) 65
BsuI GTATCC 1 cut(s) 290
BsuRI GGCC 2 cut(s) 33, 135
BtsCI GGATG 1 cut(s) 318
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 2 cut(s) 74, 451
BveI ACCTGC 1 cut(s) 70
Cac8I GCNNGC 1 cut(s) 365
Cfr10I RCCGGY 2 cut(s) 29, 395
CseI GACGC 1 cut(s) 225
Csp6I GTAC 1 cut(s) 428
CviAII CATG 3 cut(s) 275, 364, 432
CviJI RGCY 7 cut(s) 20, 33, 56, 61, 135, 322, 395
CviKI_1 RGCY 7 cut(s) 20, 33, 56, 61, 135, 322, 395
CviQI GTAC 1 cut(s) 428
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco130I CCWWGG 2 cut(s) 130, 474
Eco147I AGGCCT 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 63
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 135
EcoT14I CCWWGG 2 cut(s) 130, 474
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 2 cut(s) 130, 474
FaeI CATG 3 cut(s) 278, 367, 435
FaiI YATR 4 cut(s) 39, 276, 365, 433
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 3 cut(s) 274, 363, 431
FauI CCCGC 1 cut(s) 97
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 1 cut(s) 325
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 2 cut(s) 392, 508
GluI GCNGC 1 cut(s) 54
GsaI CCCAGC 1 cut(s) 60
GsuI CTGGAG 1 cut(s) 508
HaeIII GGCC 2 cut(s) 33, 135
HapII CCGG 2 cut(s) 30, 396
HgaI GACGC 1 cut(s) 225
Hin1II CATG 3 cut(s) 278, 367, 435
HinfI GANTC 2 cut(s) 118, 483
HpaII CCGG 2 cut(s) 30, 396
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 3 cut(s) 117, 383, 410
Hpy188III TCNNGA 1 cut(s) 487
Hpy8I GTNNAC 1 cut(s) 245
HpyCH4III ACNGT 2 cut(s) 184, 499
HpyCH4V TGCA 8 cut(s) 53, 79, 110, 206, 227, 278, 367, 425
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 85, 233
Hsp92II CATG 3 cut(s) 278, 367, 435
LguI GCTCTTC 1 cut(s) 273
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 4 cut(s) 40, 150, 303, 366
MaeI CTAG 2 cut(s) 392, 508
MaeIII GTNAC 1 cut(s) 328
MbiI CCGCTC 1 cut(s) 266
MboII GAAGA 3 cut(s) 252, 260, 263
MfeI CAATTG 1 cut(s) 207
MhlI GDGCHC 1 cut(s) 63
MluCI AATT 4 cut(s) 143, 207, 370, 384
MnlI CCTC 2 cut(s) 349, 426
MseI TTAA 1 cut(s) 141
MslI CAYNNNNRTG 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 2 cut(s) 30, 396
MspR9I CCNGG 1 cut(s) 137
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 3 cut(s) 50, 85, 233
NlaIII CATG 3 cut(s) 278, 367, 435
NmuCI GTSAC 1 cut(s) 328
NspI RCATGY 1 cut(s) 367
PaeI GCATGC 1 cut(s) 367
PceI AGGCCT 1 cut(s) 135
PciSI GCTCTTC 1 cut(s) 273
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 118, 483
PflMI CCANNNNNTGG 1 cut(s) 136
PkrI GCNGC 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 135
PspFI CCCAGC 1 cut(s) 56
PspGI CCWGG 1 cut(s) 135
PstI CTGCAG 2 cut(s) 81, 427
PvuII CAGCTG 1 cut(s) 56
RsaI GTAC 1 cut(s) 429
RsaNI GTAC 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 279
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 1 cut(s) 54
ScrFI CCNGG 1 cut(s) 137
SduI GDGCHC 1 cut(s) 63
SetI ASST 6 cut(s) 22, 58, 84, 190, 360, 509
SfaNI GCATC 4 cut(s) 40, 150, 303, 366
SfcI CTRYAG 4 cut(s) 77, 174, 183, 423
SmiMI CAYNNNNRTG 1 cut(s) 279
SphI GCATGC 1 cut(s) 367
Sse9I AATT 4 cut(s) 143, 207, 370, 384
SseBI AGGCCT 1 cut(s) 135
SsiI CCGC 2 cut(s) 104, 264
SspMI CTAG 2 cut(s) 392, 508
StuI AGGCCT 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 2 cut(s) 130, 474
TaaI ACNGT 2 cut(s) 184, 499
TaqI TCGA 2 cut(s) 354, 374
TasI AATT 4 cut(s) 143, 207, 370, 384
TfiI GAWTC 2 cut(s) 118, 483
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 2 cut(s) 81, 451
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 3 cut(s) 136, 210, 263
TspRI CASTG 2 cut(s) 81, 451
Van91I CCANNNNNTGG 1 cut(s) 136
XapI RAATTY 2 cut(s) 143, 370
XceI RCATGY 1 cut(s) 367
XspI CTAG 2 cut(s) 392, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.