Rh5CG225100

sodium metabolite cotransporter BASS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0006339
Physical Location & Seq
Reverse (-)
22967828 .. 22970775
2948 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG225100.1

Sequence Viewer

Length: 1068 bp
ATGCAGTTGACAGTACTTCCATACTTCCATGGCAACAGAGCCTTCAGATTCCAAGTAAACCCAAACCCCATTTCGAACCAATCACCACTCCGAGCTACTAAACCATCACCTTCCTCCTCTATTCAACTCACACTAAGATCCCACTCTCACTCCCAATCGCCGTCTCTCCAAAACCCCAAATGGCCCCTCACCTCAACTCCCTTCTCCTCCATCGCAGCCACCACAAAGAAGCCGCAGTCATCAGTGGCTCCAGTTCGTTCAGGCATTTCGTCGAACAGCTTCAGTGCCAATGGCAAAAGGAGCTTCAGGGACTGGGTTGAAGCGGTGAGTGAGGCTGTTTCCAATGCACTTCCGATATGGGTTGCTTTGGGGTGCCTCCTGGGCCTCATCAGTCCCGGTTCCTTCAACTGGGTCACACCCAAGCTCAATTTTTTCGGCTTAACGCTCATTATGCTTGGAATGGGTATGACCCTCACTTTCAATGACCTCAGGGACGCTTTGGCTATGCCCAAGGAGTTGTTCTCTGGCTTCTTTCTTCAGTACTCGATCATGACCCCTTTTCTCACGGCCAAACTTGCTGGTCAATATGTTGCAGTAGATGCACCTGGAATGTTATTCTCAACACTGCAGGTTGTGCTTCTTCCTGTGATGGCCGGTGCATTTCTGAATCAGTACTTCCAAGGTCTGGTTAAATTTGTCTCCCCCTTGATGCCACCCATTGCTGTGACAACAGTAGGTGTTCTTTGTGGGAACGCAATAGCCCAAAGTTCCTCCGCAATCCTCAAGTCTGGTCTACAAGTTGTGCTAGCTTCCTCCCTTCTGCATGCATCTGGATTTTTCTTTGGTTACGTGCTTTCAAGGTTACTTGGGCTTGATGTGGCATCATCAAGGACTATCTCAATTGAGGTTGGCATGCAGAATTCGGTGCTTGGAGTTGTTCTAGCTACTCAGCACTTTGGAAATCCTCTTACTGCAGTGCCATGCGCTGTTTCCAGCGTTTGCCAATCAATCATCGGTAGTGCCTTGGCAGGAATATGGAGGCGGAGCGTGCCGAAAAAGGAAGAATGA

Protein Analysis

355

Amino Acids

37.93

Weight (kDa)

9.98

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBF PF01758 145 - 190 5.7e-06 Sodium Bile acid symporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 619
AccB1I GGYRCC 1 cut(s) 372
AccB7I CCANNNNNTGG 1 cut(s) 685
AccI GTMKAC 1 cut(s) 793
AciI CCGC 4 cut(s) 233, 323, 774, 1042
AclWI GGATC 1 cut(s) 132
AcoI YGGCCR 2 cut(s) 567, 651
AcsI RAATTY 2 cut(s) 692, 919
AcuI CTGAAG 4 cut(s) 28, 265, 289, 521
AfaI GTAC 3 cut(s) 15, 542, 674
AfiI CCNNNNNNNGG 2 cut(s) 408, 685
AgsI TTSAA 5 cut(s) 125, 320, 406, 481, 858
AjnI CCWGG 2 cut(s) 378, 604
AjuI GAANNNNNNNTTGG 4 cut(s) 55, 87, 503, 535
AluBI AGCT 6 cut(s) 95, 279, 303, 424, 809, 944
AluI AGCT 6 cut(s) 95, 279, 303, 424, 809, 944
Alw26I GTCTC 2 cut(s) 168, 703
AlwI GGATC 1 cut(s) 132
AlwNI CAGNNNCTG 1 cut(s) 312
AoxI GGCC 4 cut(s) 182, 382, 567, 651
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 2 cut(s) 692, 919
Asp700I GAANNNNTTC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 986
AspS9I GGNCC 2 cut(s) 183, 382
AsuC2I CCSGG 1 cut(s) 396
AsuHPI GGTGA 4 cut(s) 75, 99, 181, 337
AsuII TTCGAA 1 cut(s) 74
AsuNHI GCTAGC 1 cut(s) 805
AxyI CCTNAGG 1 cut(s) 488
BanI GGYRCC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 227
BccI CCATC 3 cut(s) 112, 218, 643
BceAI ACGGC 2 cut(s) 145, 582
BciT130I CCWGG 2 cut(s) 380, 606
BcnI CCSGG 1 cut(s) 396
BcoDI GTCTC 2 cut(s) 168, 703
BfaI CTAG 2 cut(s) 806, 941
BfmI CTRYAG 2 cut(s) 626, 972
BfuAI ACCTGC 1 cut(s) 619
BglI GCCNNNNNGGC 1 cut(s) 381
BisI GCNGC 2 cut(s) 216, 233
BlsI GCNGC 2 cut(s) 217, 234
BmcAI AGTACT 3 cut(s) 15, 542, 674
Bme1390I CCNGG 3 cut(s) 380, 396, 606
BmgT120I GGNCC 2 cut(s) 183, 382
BmiI GGNNCC 4 cut(s) 185, 249, 374, 400
BmrFI CCNGG 3 cut(s) 380, 396, 606
BmrI ACTGGG 2 cut(s) 322, 418
BmsI GCATC 4 cut(s) 589, 699, 836, 890
BmtI GCTAGC 1 cut(s) 809
BmuI ACTGGG 2 cut(s) 322, 418
BplI GAGNNNNNCTC 2 cut(s) 506, 538
BpmI CTGGAG 1 cut(s) 234
Bpu14I TTCGAA 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 767
BpuMI CCSGG 1 cut(s) 396
BsaAI YACGTR 1 cut(s) 850
BsaJI CCNNGG 5 cut(s) 28, 379, 510, 679, 1023
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 181, 211
Bsc4I CCNNNNNNNGG 2 cut(s) 408, 685
Bse118I RCCGGY 1 cut(s) 653
Bse1I ACTGG 3 cut(s) 251, 317, 413
Bse21I CCTNAGG 1 cut(s) 488
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 2 cut(s) 380, 606
BseDI CCNNGG 5 cut(s) 28, 379, 510, 679, 1023
BseLI CCNNNNNNNGG 2 cut(s) 408, 685
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 2 cut(s) 502, 962
BseNI ACTGG 3 cut(s) 251, 317, 413
BseRI GAGGAG 2 cut(s) 106, 196
BseXI GCAGC 1 cut(s) 227
BshFI GGCC 4 cut(s) 184, 384, 569, 653
BshNI GGYRCC 1 cut(s) 372
BsiSI CCGG 2 cut(s) 396, 654
BslFI GGGAC 3 cut(s) 323, 378, 506
BslI CCNNNNNNNGG 2 cut(s) 408, 685
BsmAI GTCTC 2 cut(s) 168, 703
BsmBI CGTCTC 1 cut(s) 168
BsmFI GGGAC 3 cut(s) 323, 378, 506
BsnI GGCC 4 cut(s) 184, 384, 569, 653
Bsp119I TTCGAA 1 cut(s) 74
Bsp143I GATC 2 cut(s) 137, 546
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 4 cut(s) 233, 323, 774, 1042
BspANI GGCC 4 cut(s) 184, 384, 569, 653
BspCNI CTCAG 2 cut(s) 501, 961
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 4 cut(s) 185, 249, 374, 400
BspMAI CTGCAG 2 cut(s) 630, 976
BspMI ACCTGC 1 cut(s) 619
BspOI GCTAGC 1 cut(s) 809
BspPI GGATC 1 cut(s) 132
BspT104I TTCGAA 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 717
BsrFI RCCGGY 1 cut(s) 653
BsrI ACTGG 3 cut(s) 251, 317, 413
BssAI RCCGGY 1 cut(s) 653
BssECI CCNNGG 5 cut(s) 28, 379, 510, 679, 1023
BssMI GATC 2 cut(s) 137, 546
BssT1I CCWWGG 4 cut(s) 28, 510, 679, 1023
Bst2UI CCWGG 2 cut(s) 380, 606
Bst4CI ACNGT 2 cut(s) 13, 733
BstAPI GCANNNNNTGC 2 cut(s) 599, 634
BstBAI YACGTR 1 cut(s) 850
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 807, 825, 914, 1049
BstDEI CTNAG 3 cut(s) 134, 488, 948
BstDSI CCRYGG 1 cut(s) 28
BstHHI GCGC 1 cut(s) 986
BstKTI GATC 2 cut(s) 140, 549
BstMAI GTCTC 2 cut(s) 168, 703
BstMBI GATC 2 cut(s) 137, 546
BstMWI GCNNNNNNNGC 7 cut(s) 300, 381, 451, 575, 599, 634, 1048
BstNI CCWGG 2 cut(s) 380, 606
BstNSI RCATGY 2 cut(s) 827, 916
BstSCI CCNGG 3 cut(s) 378, 394, 604
BstSFI CTRYAG 2 cut(s) 626, 972
BstV1I GCAGC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 137
BstYI RGATCY 1 cut(s) 137
Bsu36I CCTNAGG 1 cut(s) 488
BsuRI GGCC 4 cut(s) 184, 384, 569, 653
BtgI CCRYGG 1 cut(s) 28
BtgZI GCGATG 1 cut(s) 196
BtsI GCAGTG 2 cut(s) 623, 981
BtsIMutI CAGTG 4 cut(s) 249, 289, 623, 981
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 4 cut(s) 807, 825, 914, 1049
CaiI CAGNNNCTG 1 cut(s) 312
CciI TCATGA 1 cut(s) 549
CfoI GCGC 1 cut(s) 986
Cfr10I RCCGGY 1 cut(s) 653
Cfr13I GGNCC 2 cut(s) 183, 382
CseI GACGC 1 cut(s) 503
Csp6I GTAC 3 cut(s) 14, 541, 673
CviAII CATG 5 cut(s) 29, 550, 824, 913, 981
CviQI GTAC 3 cut(s) 14, 541, 673
DdeI CTNAG 3 cut(s) 134, 488, 948
DpnI GATC 2 cut(s) 139, 548
DpnII GATC 2 cut(s) 137, 546
EaeI YGGCCR 2 cut(s) 567, 651
EciI GGCGGA 1 cut(s) 1057
Eco130I CCWWGG 4 cut(s) 28, 510, 679, 1023
Eco57I CTGAAG 4 cut(s) 28, 265, 289, 521
Eco81I CCTNAGG 1 cut(s) 488
EcoRI GAATTC 1 cut(s) 919
EcoRII CCWGG 2 cut(s) 378, 604
EcoT14I CCWWGG 4 cut(s) 28, 510, 679, 1023
EcoT22I ATGCAT 1 cut(s) 829
ErhI CCWWGG 4 cut(s) 28, 510, 679, 1023
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 5 cut(s) 32, 553, 827, 916, 984
FaqI GGGAC 3 cut(s) 323, 378, 506
FatI CATG 5 cut(s) 28, 549, 823, 912, 980
FblI GTMKAC 1 cut(s) 793
Fnu4HI GCNGC 2 cut(s) 216, 233
Fsp4HI GCNGC 2 cut(s) 216, 233
FspBI CTAG 2 cut(s) 806, 941
GlaI GCGC 1 cut(s) 985
GluI GCNGC 2 cut(s) 216, 233
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 4 cut(s) 184, 384, 569, 653
HapII CCGG 2 cut(s) 396, 654
HgaI GACGC 1 cut(s) 503
HhaI GCGC 1 cut(s) 986
Hin1II CATG 5 cut(s) 32, 553, 827, 916, 984
Hin6I GCGC 1 cut(s) 984
HinP1I GCGC 1 cut(s) 984
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 2 cut(s) 48, 667
HpaII CCGG 2 cut(s) 396, 654
HphI GGTGA 4 cut(s) 75, 99, 181, 337
Hpy166II GTNNAC 3 cut(s) 9, 58, 794
Hpy188I TCNGA 4 cut(s) 47, 92, 354, 666
Hpy188III TCNNGA 2 cut(s) 550, 831
Hpy8I GTNNAC 3 cut(s) 9, 58, 794
Hpy99I CGWCG 1 cut(s) 274
HpyAV CCTTC 5 cut(s) 52, 120, 211, 412, 827
HpyCH4III ACNGT 2 cut(s) 13, 733
HpyCH4IV ACGT 1 cut(s) 849
HpyF10VI GCNNNNNNNGC 7 cut(s) 300, 381, 451, 575, 599, 634, 1048
HpyF3I CTNAG 3 cut(s) 134, 488, 948
HpySE526I ACGT 1 cut(s) 849
Hsp92II CATG 5 cut(s) 32, 553, 827, 916, 984
HspAI GCGC 1 cut(s) 984
Kzo9I GATC 2 cut(s) 137, 546
LmnI GCTCC 3 cut(s) 253, 300, 1044
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 4 cut(s) 589, 699, 836, 890
MaeI CTAG 2 cut(s) 806, 941
MaeII ACGT 1 cut(s) 849
MaeIII GTNAC 4 cut(s) 412, 724, 845, 861
MalI GATC 2 cut(s) 139, 548
MboI GATC 2 cut(s) 137, 546
MboII GAAGA 2 cut(s) 527, 632
MfeI CAATTG 1 cut(s) 900
MflI RGATCY 1 cut(s) 137
MluCI AATT 4 cut(s) 427, 692, 900, 919
Mph1103I ATGCAT 1 cut(s) 829
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 2 cut(s) 440, 690
MslI CAYNNNNRTG 1 cut(s) 722
MspI CCGG 2 cut(s) 396, 654
MspR9I CCNGG 3 cut(s) 380, 396, 606
MunI CAATTG 1 cut(s) 900
MvaI CCWGG 2 cut(s) 380, 606
MwoI GCNNNNNNNGC 7 cut(s) 300, 381, 451, 575, 599, 634, 1048
NciI CCSGG 1 cut(s) 396
NcoI CCATGG 1 cut(s) 28
NdeII GATC 2 cut(s) 137, 546
NheI GCTAGC 1 cut(s) 805
NlaIII CATG 5 cut(s) 32, 553, 827, 916, 984
NlaIV GGNNCC 4 cut(s) 185, 249, 374, 400
NmuCI GTSAC 2 cut(s) 412, 724
NsiI ATGCAT 1 cut(s) 829
NspI RCATGY 2 cut(s) 827, 916
NspV TTCGAA 1 cut(s) 74
PaeI GCATGC 2 cut(s) 827, 916
PagI TCATGA 1 cut(s) 549
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 2 cut(s) 48, 667
PflMI CCANNNNNTGG 1 cut(s) 685
PkrI GCNGC 2 cut(s) 217, 234
Ppu21I YACGTR 1 cut(s) 850
Psp6I CCWGG 2 cut(s) 378, 604
PspGI CCWGG 2 cut(s) 378, 604
PspN4I GGNNCC 4 cut(s) 185, 249, 374, 400
PspPI GGNCC 2 cut(s) 183, 382
PstI CTGCAG 2 cut(s) 630, 976
PstNI CAGNNNCTG 1 cut(s) 312
PsuI RGATCY 1 cut(s) 137
RsaI GTAC 3 cut(s) 15, 542, 674
RsaNI GTAC 3 cut(s) 14, 541, 673
RseI CAYNNNNRTG 1 cut(s) 722
SaqAI TTAA 2 cut(s) 440, 690
SatI GCNGC 2 cut(s) 216, 233
Sau3AI GATC 2 cut(s) 137, 546
Sau96I GGNCC 2 cut(s) 183, 382
ScaI AGTACT 3 cut(s) 15, 542, 674
ScrFI CCNGG 3 cut(s) 380, 396, 606
SfaNI GCATC 4 cut(s) 589, 699, 836, 890
SfcI CTRYAG 2 cut(s) 626, 972
SfuI TTCGAA 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 722
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
SphI GCATGC 2 cut(s) 827, 916
Sse9I AATT 4 cut(s) 427, 692, 900, 919
SsiI CCGC 4 cut(s) 233, 323, 774, 1042
SspMI CTAG 2 cut(s) 806, 941
StyD4I CCNGG 3 cut(s) 378, 394, 604
StyI CCWWGG 4 cut(s) 28, 510, 679, 1023
TaaI ACNGT 2 cut(s) 13, 733
TaiI ACGT 1 cut(s) 852
TaqI TCGA 3 cut(s) 74, 272, 545
TasI AATT 4 cut(s) 427, 692, 900, 919
TatI WGTACW 3 cut(s) 13, 540, 672
TauI GCSGC 1 cut(s) 235
TfiI GAWTC 2 cut(s) 48, 667
Tru1I TTAA 2 cut(s) 440, 690
Tru9I TTAA 2 cut(s) 440, 690
TscAI CASTG 4 cut(s) 249, 289, 630, 981
TseFI GTSAC 2 cut(s) 412, 724
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 2 cut(s) 412, 724
TspRI CASTG 4 cut(s) 249, 289, 630, 981
Van91I CCANNNNNTGG 1 cut(s) 685
XapI RAATTY 2 cut(s) 692, 919
XceI RCATGY 2 cut(s) 827, 916
XmiI GTMKAC 1 cut(s) 793
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 2 cut(s) 806, 941
ZrmI AGTACT 3 cut(s) 15, 542, 674
Zsp2I ATGCAT 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.