pycom12g03600

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007834
Physical Location & Seq
Reverse (-)
3511077 .. 3517621
6545 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3132 bp
ATGTCAGTGCGCTTCCTCCTTGGCGCAACAAAAAGCTGCCCGCTTCCCCGACACCTCCTCGCCGCGCCCAAAATCTTCGCGTTTCGCCCTGGGATCTCGACACTCGCCATGTCCGGACGCAACAACTTCCAAGGCGGTCGCCGAGGCGGCCCTAACTCTGGCGGAGGCCGCCGCGGTCGCGGTGGCGGCGGCAGAGGTGGAGGCGGTCGTGGAGGTGGAGGTGGAGGCGGCCGCGGCGGAGAACAGCGTTGGTGGGACCCGGTATGGCGCGCAGAGCGACTGAGGCAGCAGGCCGTCCAGATGGAAGTGCTTGATGAGACGGAATGGTGGGGAAAGATGGAGCAGATGAAGAACGGAGCGGAGCAGGAGATGGTAATTAAGCGTAATTTTAGCCGGAATGATCAGCAAATTCTATATGACATGGCTTATCAACTGGGGCTTTACTTCCATGCATACAATAAAGGGAAGGCTTTGGTGGTCAGCAAAGTTCCATTGCCGGACTACCGGGCAGATCTTGATGAGCGCCATGGTTCCACACAGAAAGAGATTAAAATGTCTACAGAGACTGCCAATAGAGTTGGGAGTCTTTTGCACAGCTCGCCGTCACAGGGGGAAATATCTGTCAATGGGCCTTCTGGGTCAGGGCAGGGCAACAGACAAACTTCAGCTAGCGTAATTACGAGTAAACCTGTCGCCCAGTTAGAGCCTGATAATGTTAATGAGAAAGAGAAACTCAGCCTTCAACTTAAGGAGAAACAGGAAAAAATGAAGGTGAGCAATAGTTTAAAAGCAATGCAATCGTTTCGAGAAAAGCTTCCTGCTTTCAAAATGAAGTCCGAGTTTCTGAAAGCTGTTTCAGAAAATCAGGTCTTGGTAGTCTCTGGGGAGACAGGTTGCGGAAAAACAACACAGCTTCCACAATTTATTCTAGAAAATGAGATCTCACGTCTGCATGGTGCTGACTGCAACATAATATGCACACAACCTCGTCGTATTTCTGCTGTATCTGTTGCAGCTCGTATATCCTCTGAAAGGGGAGAGAATCTTGGTGAAACTGTTGGTTATCAGATCCGTCTTGAATCAAAGCGCTCTGCTCAAACACGACTGTTATTTTGCACCACTGGAGTGTTACTTCGACAGTTGGTTCAAGACCCAGAATTAACGGGCGTGAGCCACTTGCTGGTTGATGAAATTCATGAAAGAGGCATGAATGAGGACTTTTTACTCATAATCTTGCGTGACCTTCTTCCTCGGCGTCCAGATCTACGATTAATTCTAATGAGTGCTACCATCAATGCTGACTTGTTCTCCAGATACTTTGGAAATTCCCCAACAATACATATACCAGGATTAACTTTTCCTGTGGCAGAGTTATTTCTAGAAGATATTCTGGAGAAAACTCGTTACGCTGTCAAGTCAGAGTTTGACAACTTTGAGGTGGGGAATTCCAGGAGAAGGAGACAGCAAGATTCCAAGAAAGATCCTTTAACTGAATTATTTGAGGATGCCGATATTGATGTTCAGTTCAAAAATTACAGCACAGCTACAAGAAAGTCTCTTGAAGCTTGGTCTGGTTCACAGCTTGATTTGGGGCTGGTGGAGGCGACAATTGAACATATATGTCGCAATGAGAGAGATGGAGCAATTCTTGTATTCCTTACGGGCTGGGATGACATATCAAAGTTACTTGACAAAATTAAGGGAAATAGATTCCTTGGAGATCCCACAAAATATATGGTTCTTCCCCTGCATGGGTCAATGCCTACCGTCAATCAACGAGAGATATTTGATCGTCCTCCCCCTAATAAAAGAAAAATTGTGCTGGCAACAAACATAGCTGAGAGTAGTATCACCATAGATGACGTTGTGTATGTCATAGACTGCGGAAAGGCAAAGGAAACCAGTTATGATGCTTTAAACAAGTTGGCTTGTCTATTACCATCATGGATTTCAAAGGCTTCAGCACATCAGAGGCGAGGGCGTGCTGGCCGTGTGCAACCTGGAGTTTGTTACAGACTATATCCGAAAATGATCCATGATGCAATGCTCCAATATCAATTACCTGAAATCCTCCGGACGCCGTTGCAAGAGCTATGCCTCCACATCAAAAGTTTGCAACTTGGAGCTGTTGGGTCATTTTTGGCAAAGGCACTTCAGCCACCAGATTCTCTTGCAGTTCAAAATGCAATTGAACTTCTCAAAACCATTGGAGCTTTAGATGACACAGAGGAGCTTACCCCGCTTGGTCGCCATCTTTGCACGCTACCATTGGATCCAAACATTGGGAAGATGCTTTTAATGGGTTCTATATTTCAATGCCTCAATCCTGCTTTAACAATTGCAGCTGCTCTTGCTCATCGTGACCCATTTATACTACCAATAAATAAGAAAGAGGATGCTGATGCTGCAAAGAGGTCCTTTGCGGGTGATTCTTTCAGTGATCACATAGCTCTTGTGAAAGCTTTTGAAGGATGGAAAGATGCAAAACAAAATGGAGCGGGAGCAGGAAAGTCGTTCTGCTGGGAGAACTTTTTATCCCCTGTAACATTGCAGATGATGGAAGATATGAGGATCCAGTTTCATGATCTACTATCAAATATTGGCTTTCTTGACAAATCCAGGGGTGCTAATGCTTACAATCAATACAGCCATGACTTGGAGATGGTGTGTGCGGTCCTGTGTGCTGGGCTCTATCCGAATGTCGTTCAGTGTAAAAGAAGAGGAAAGCGGACAGCATTCTATACTAAAGAAGTTGGTAAAGTTGATATCCATCCTGCATCAGTCAATGCTGGGGTTCATTTATTCCCTCTCCCTTACATGGTTTATAGTGAAAAAGTGAAGACAACCAGCATTTACATCCGAGACTCTACGAATTTATCAGATTATGCTCTACTTCTATTTGGTGGTAGTCTTATCCCTAGCAAGACCGGGGAGGGCATTGAGATGCTCGGAGGTTACCTTCATTTCTCCGCATCAAAGAGTGTTCTAGAATTGATTCGGAAACTGCGTGGTGAACTCGACAAGCTTTTGAACAGGAAGATTGACAACCCAGGATTGGACATCTCCAGCGAGGGAAAGGCAGTTGTGTCTGCTGTGGTAGAGTTGCTGCATAGTCAGAATGTACAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1044

Amino Acids

115.67

Weight (kDa)

8.95

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 279 - 433 1.2e-09 DEAD/DEAH box helicase
Helicase_C PF00271 535 - 665 1.7e-15 Helicase conserved C-terminal domain
WHD_HA2 PF04408 729 - 757 1.3e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 758 - 818 1.1e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 889 - 970 2.5e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 980 - 1037 2.9e-22 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 1180, 2282, 2656
AccBSI CCGCTC 2 cut(s) 359, 2498
AccI GTMKAC 1 cut(s) 557
AccII CGCG 6 cut(s) 65, 80, 174, 180, 234, 270
AccIII TCCGGA 2 cut(s) 113, 2073
AclWI GGATC 9 cut(s) 101, 1063, 1475, 1715, 2026, 2267, 2280, 2566, 2579
AcoI YGGCCR 2 cut(s) 229, 1987
AcsI RAATTY 5 cut(s) 408, 1191, 1324, 1444, 2872
AcuI CTGAAG 3 cut(s) 648, 1944, 2138
AcyI GRCGYC 2 cut(s) 1255, 2078
AfaI GTAC 1 cut(s) 3123
AfeI AGCGCT 1 cut(s) 1088
AflII CTTAAG 1 cut(s) 746
AjiI CACGTC 1 cut(s) 947
AjnI CCWGG 6 cut(s) 88, 1345, 1448, 1999, 2618, 3049
AleI CACNNNNGTG 1 cut(s) 1124
AloI GAACNNNNNNTCC 2 cut(s) 2519, 2551
Alw26I GTCTC 7 cut(s) 311, 557, 881, 883, 1453, 1560, 2856
AlwI GGATC 9 cut(s) 101, 1063, 1475, 1715, 2026, 2267, 2280, 2566, 2579
AlwNI CAGNNNCTG 1 cut(s) 566
Aor13HI TCCGGA 2 cut(s) 113, 2073
Aor51HI AGCGCT 1 cut(s) 1088
AoxI GGCC 6 cut(s) 148, 166, 229, 291, 629, 1987
ApeKI GCWGC 7 cut(s) 36, 286, 1013, 2342, 2345, 2405, 3106
ApoI RAATTY 5 cut(s) 408, 1191, 1324, 1444, 2872
ArsI GACNNNNNNTTYG 2 cut(s) 1095, 1127
AseI ATTAAT 1 cut(s) 1271
Asp700I GAANNNNTTC 3 cut(s) 813, 1386, 2994
AspLEI GCGC 7 cut(s) 12, 26, 67, 270, 272, 525, 1089
AspS9I GGNCC 5 cut(s) 149, 256, 629, 2415, 2674
AsuC2I CCSGG 3 cut(s) 260, 506, 2929
AsuHPI GGTGA 5 cut(s) 784, 1061, 1843, 2438, 3023
AsuNHI GCTAGC 1 cut(s) 668
AvaII GGWCC 3 cut(s) 256, 2415, 2674
BamHI GGATCC 2 cut(s) 2272, 2571
BanII GRGCYC 1 cut(s) 2691
BbsI GAAGAC 1 cut(s) 2846
BbvI GCAGC 7 cut(s) 23, 298, 1025, 2332, 2354, 2392, 3093
BceAI ACGGC 4 cut(s) 278, 586, 1974, 2065
BcgI CGANNNNNNTGC 6 cut(s) 780, 785, 814, 819, 2493, 2527
BciT130I CCWGG 6 cut(s) 90, 1347, 1450, 2001, 2620, 3051
BclI TGATCA 2 cut(s) 400, 2440
BcnI CCSGG 3 cut(s) 260, 506, 2929
BcoDI GTCTC 7 cut(s) 311, 557, 881, 883, 1453, 1560, 2856
BfaI CTAG 5 cut(s) 669, 929, 1379, 2919, 2987
BfmI CTRYAG 1 cut(s) 558
BfoI RGCGCY 2 cut(s) 526, 1090
BfrI CTTAAG 1 cut(s) 746
BglI GCCNNNNNGGC 1 cut(s) 147
BglII AGATCT 3 cut(s) 511, 939, 1261
Bme1390I CCNGG 9 cut(s) 90, 260, 506, 1347, 1450, 2001, 2620, 2929, 3051
Bme18I GGWCC 3 cut(s) 256, 2415, 2674
BmgBI CACGTC 1 cut(s) 947
BmgT120I GGNCC 5 cut(s) 149, 256, 629, 2415, 2674
BmiI GGNNCC 5 cut(s) 257, 258, 532, 2274, 2573
BmrFI CCNGG 9 cut(s) 90, 260, 506, 1347, 1450, 2001, 2620, 2929, 3051
BmrI ACTGGG 2 cut(s) 443, 691
BmtI GCTAGC 1 cut(s) 672
BmuI ACTGGG 2 cut(s) 443, 691
BpiI GAAGAC 1 cut(s) 2846
BpmI CTGGAG 5 cut(s) 1143, 1294, 1412, 2022, 3049
BpuMI CCSGG 3 cut(s) 260, 506, 2929
BsaBI GATNNNNATC 1 cut(s) 2769
BsaHI GRCGYC 2 cut(s) 1255, 2078
BsaWI WCCGGW 2 cut(s) 113, 2073
BsaXI ACNNNNNCTCC 4 cut(s) 1292, 1322, 2651, 2681
Bse1I ACTGG 5 cut(s) 438, 697, 1126, 1902, 2575
Bse3DI GCAATG 5 cut(s) 491, 798, 1633, 2049, 2546
Bse8I GATNNNNATC 1 cut(s) 2769
BseAI TCCGGA 2 cut(s) 113, 2073
BseBI CCWGG 6 cut(s) 90, 1347, 1450, 2001, 2620, 3051
BseGI GGATG 6 cut(s) 1510, 1675, 2401, 2477, 2770, 2856
BseJI GATNNNNATC 1 cut(s) 2769
BseMI GCAATG 5 cut(s) 491, 798, 1633, 2049, 2546
BseMII CTCAG 3 cut(s) 272, 748, 1830
BseNI ACTGG 5 cut(s) 438, 697, 1126, 1902, 2575
BsePI GCGCGC 1 cut(s) 268
BseRI GAGGAG 2 cut(s) 47, 2243
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 7 cut(s) 23, 298, 1025, 2332, 2354, 2392, 3093
BseYI CCCAGC 4 cut(s) 1665, 2520, 2684, 2789
Bsh1236I CGCG 6 cut(s) 65, 80, 174, 180, 234, 270
Bsh1285I CGRYCG 4 cut(s) 139, 178, 208, 232
BshFI GGCC 6 cut(s) 150, 168, 231, 293, 631, 1989
BsiEI CGRYCG 4 cut(s) 139, 178, 208, 232
BsiSI CCGG 7 cut(s) 114, 260, 394, 497, 505, 2074, 2928
BslFI GGGAC 1 cut(s) 269
BsmAI GTCTC 7 cut(s) 311, 557, 881, 883, 1453, 1560, 2856
BsmBI CGTCTC 1 cut(s) 311
BsmFI GGGAC 1 cut(s) 269
BsmI GAATGC 1 cut(s) 2735
BsnI GGCC 6 cut(s) 150, 168, 231, 293, 631, 1989
Bsp1286I GDGCHC 1 cut(s) 2691
Bsp13I TCCGGA 2 cut(s) 113, 2073
Bsp1407I TGTACA 1 cut(s) 3121
Bsp19I CCATGG 1 cut(s) 526
BspANI GGCC 6 cut(s) 150, 168, 231, 293, 631, 1989
BspCNI CTCAG 3 cut(s) 273, 747, 1831
BspEI TCCGGA 2 cut(s) 113, 2073
BspFNI CGCG 6 cut(s) 65, 80, 174, 180, 234, 270
BspHI TCATGA 2 cut(s) 1195, 2581
BspLI GGNNCC 5 cut(s) 257, 258, 532, 2274, 2573
BspOI GCTAGC 1 cut(s) 672
BspPI GGATC 9 cut(s) 101, 1063, 1475, 1715, 2026, 2267, 2280, 2566, 2579
BspTI CTTAAG 1 cut(s) 746
BsrBI CCGCTC 2 cut(s) 359, 2498
BsrDI GCAATG 5 cut(s) 491, 798, 1633, 2049, 2546
BsrGI TGTACA 1 cut(s) 3121
BsrI ACTGG 5 cut(s) 438, 697, 1126, 1902, 2575
BssHII GCGCGC 1 cut(s) 268
BssNI GRCGYC 2 cut(s) 1255, 2078
BssT1I CCWWGG 4 cut(s) 19, 130, 526, 1714
Bst2UI CCWGG 6 cut(s) 90, 1347, 1450, 2001, 2620, 3051
Bst4CI ACNGT 4 cut(s) 1057, 1107, 1140, 1768
Bst6I CTCTTC 1 cut(s) 2713
BstACI GRCGYC 2 cut(s) 1255, 2078
BstAFI CTTAAG 1 cut(s) 746
BstAUI TGTACA 1 cut(s) 3121
BstC8I GCNNGC 9 cut(s) 41, 270, 291, 599, 670, 1824, 1983, 1987, 2261
BstDEI CTNAG 3 cut(s) 281, 734, 1839
BstDSI CCRYGG 3 cut(s) 172, 232, 526
BstEII GGTNACC 1 cut(s) 2954
BstENI CCTNNNNNAGG 1 cut(s) 1030
BstF5I GGATG 6 cut(s) 1510, 1675, 2401, 2477, 2770, 2856
BstFNI CGCG 6 cut(s) 65, 80, 174, 180, 234, 270
BstH2I RGCGCY 2 cut(s) 526, 1090
BstHHI GCGC 7 cut(s) 12, 26, 67, 270, 272, 525, 1089
BstMAI GTCTC 7 cut(s) 311, 557, 881, 883, 1453, 1560, 2856
BstMCI CGRYCG 4 cut(s) 139, 178, 208, 232
BstNI CCWGG 6 cut(s) 90, 1347, 1450, 2001, 2620, 3051
BstPI GGTNACC 1 cut(s) 2954
BstSCI CCNGG 9 cut(s) 88, 258, 504, 1345, 1448, 1999, 2618, 2927, 3049
BstSFI CTRYAG 1 cut(s) 558
BstUI CGCG 6 cut(s) 65, 80, 174, 180, 234, 270
BstV1I GCAGC 7 cut(s) 23, 298, 1025, 2332, 2354, 2392, 3093
BstV2I GAAGAC 1 cut(s) 2846
BstX2I RGATCY 9 cut(s) 93, 511, 939, 1068, 1261, 1480, 1720, 2272, 2571
BstYI RGATCY 9 cut(s) 93, 511, 939, 1068, 1261, 1480, 1720, 2272, 2571
BstZI CGGCCG 1 cut(s) 229
BsuRI GGCC 6 cut(s) 150, 168, 231, 293, 631, 1989
BtgI CCRYGG 3 cut(s) 172, 232, 526
BtrI CACGTC 1 cut(s) 947
BtsCI GGATG 6 cut(s) 1510, 1675, 2401, 2477, 2770, 2856
BtsIMutI CAGTG 4 cut(s) 12, 1119, 2443, 2714
Cac8I GCNNGC 9 cut(s) 41, 270, 291, 599, 670, 1824, 1983, 1987, 2261
CaiI CAGNNNCTG 1 cut(s) 566
CciI TCATGA 2 cut(s) 1195, 2581
CciNI GCGGCCGC 1 cut(s) 229
CfoI GCGC 7 cut(s) 12, 26, 67, 270, 272, 525, 1089
Cfr13I GGNCC 5 cut(s) 149, 256, 629, 2415, 2674
Cfr42I CCGCGG 2 cut(s) 175, 235
CseI GACGC 3 cut(s) 126, 1244, 2086
Csp6I GTAC 1 cut(s) 3122
CviQI GTAC 1 cut(s) 3122
DdeI CTNAG 3 cut(s) 281, 734, 1839
DraI TTTAAA 2 cut(s) 786, 1917
EaeI YGGCCR 2 cut(s) 229, 1987
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 2713
EarI CTCTTC 1 cut(s) 2713
EciI GGCGGA 2 cut(s) 177, 252
EclXI CGGCCG 1 cut(s) 229
Eco130I CCWWGG 4 cut(s) 19, 130, 526, 1714
Eco24I GRGCYC 1 cut(s) 2691
Eco32I GATATC 1 cut(s) 2767
Eco47I GGWCC 3 cut(s) 256, 2415, 2674
Eco47III AGCGCT 1 cut(s) 1088
Eco52I CGGCCG 1 cut(s) 229
Eco57I CTGAAG 3 cut(s) 648, 1944, 2138
Eco91I GGTNACC 1 cut(s) 2954
EcoNI CCTNNNNNAGG 1 cut(s) 1030
EcoO109I RGGNCCY 2 cut(s) 256, 2415
EcoO65I GGTNACC 1 cut(s) 2954
EcoRI GAATTC 1 cut(s) 1444
EcoRII CCWGG 6 cut(s) 88, 1345, 1448, 1999, 2618, 3049
EcoRV GATATC 1 cut(s) 2767
EcoT14I CCWWGG 4 cut(s) 19, 130, 526, 1714
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 2691
ErhI CCWWGG 4 cut(s) 19, 130, 526, 1714
Esp3I CGTCTC 1 cut(s) 311
FalI AAGNNNNNCTT 2 cut(s) 2402, 2434
FaqI GGGAC 1 cut(s) 269
FauI CCCGC 4 cut(s) 48, 2247, 2416, 2491
FbaI TGATCA 2 cut(s) 400, 2440
FblI GTMKAC 1 cut(s) 557
FokI GGATG 6 cut(s) 1517, 1682, 2408, 2484, 2757, 2843
FriOI GRGCYC 1 cut(s) 2691
FspBI CTAG 5 cut(s) 669, 929, 1379, 2919, 2987
GlaI GCGC 7 cut(s) 11, 25, 66, 269, 271, 524, 1088
GsaI CCCAGC 4 cut(s) 1669, 2524, 2688, 2793
GsuI CTGGAG 5 cut(s) 1143, 1294, 1412, 2022, 3049
HaeII RGCGCY 2 cut(s) 526, 1090
HaeIII GGCC 6 cut(s) 150, 168, 231, 293, 631, 1989
HapII CCGG 7 cut(s) 114, 260, 394, 497, 505, 2074, 2928
HgaI GACGC 3 cut(s) 126, 1244, 2086
HhaI GCGC 7 cut(s) 12, 26, 67, 270, 272, 525, 1089
Hin1I GRCGYC 2 cut(s) 1255, 2078
Hin6I GCGC 7 cut(s) 10, 24, 65, 268, 270, 523, 1087
HinP1I GCGC 7 cut(s) 10, 24, 65, 268, 270, 523, 1087
HindIII AAGCTT 4 cut(s) 812, 1563, 2460, 3023
HinfI GANTC 9 cut(s) 583, 1042, 1079, 1469, 1710, 2165, 2429, 2864, 2995
HpaII CCGG 7 cut(s) 114, 260, 394, 497, 505, 2074, 2928
HphI GGTGA 5 cut(s) 784, 1061, 1843, 2438, 3023
Hpy166II GTNNAC 4 cut(s) 558, 686, 1577, 3014
Hpy8I GTNNAC 4 cut(s) 558, 686, 1577, 3014
Hpy99I CGWCG 1 cut(s) 993
HpyAV CCTTC 8 cut(s) 460, 642, 749, 763, 1253, 1449, 2462, 2969
HpyCH4III ACNGT 4 cut(s) 1057, 1107, 1140, 1768
HpyCH4IV ACGT 2 cut(s) 946, 1863
HpyF3I CTNAG 3 cut(s) 281, 734, 1839
HpySE526I ACGT 2 cut(s) 946, 1863
Hsp92I GRCGYC 2 cut(s) 1255, 2078
HspAI GCGC 7 cut(s) 10, 24, 65, 268, 270, 523, 1087
KflI GGGWCCC 1 cut(s) 256
Kpn2I TCCGGA 2 cut(s) 113, 2073
Ksp22I TGATCA 2 cut(s) 400, 2440
KspI CCGCGG 2 cut(s) 175, 235
Lsp1109I GCAGC 7 cut(s) 23, 298, 1025, 2332, 2354, 2392, 3093
MaeI CTAG 5 cut(s) 669, 929, 1379, 2919, 2987
MaeII ACGT 2 cut(s) 946, 1863
MaeIII GTNAC 9 cut(s) 603, 1128, 1238, 1403, 1683, 2009, 2360, 2542, 2954
MbiI CCGCTC 2 cut(s) 359, 2498
MfeI CAATTG 3 cut(s) 1608, 2187, 2337
MflI RGATCY 9 cut(s) 93, 511, 939, 1068, 1261, 1480, 1720, 2272, 2571
MhlI GDGCHC 1 cut(s) 2691
MlyI GAGTC 2 cut(s) 592, 2858
Mph1103I ATGCAT 1 cut(s) 454
MroI TCCGGA 2 cut(s) 113, 2073
MroXI GAANNNNTTC 3 cut(s) 813, 1386, 2994
MslI CAYNNNNRTG 2 cut(s) 1124, 2942
MspA1I CMGCKG 3 cut(s) 174, 234, 2345
MspCI CTTAAG 1 cut(s) 746
MspI CCGG 7 cut(s) 114, 260, 394, 497, 505, 2074, 2928
MspR9I CCNGG 9 cut(s) 90, 260, 506, 1347, 1450, 2001, 2620, 2929, 3051
MunI CAATTG 3 cut(s) 1608, 2187, 2337
Mva1269I GAATGC 1 cut(s) 2735
MvaI CCWGG 6 cut(s) 90, 1347, 1450, 2001, 2620, 3051
MvnI CGCG 6 cut(s) 65, 80, 174, 180, 234, 270
NciI CCSGG 3 cut(s) 260, 506, 2929
NcoI CCATGG 1 cut(s) 526
NheI GCTAGC 1 cut(s) 668
NlaIV GGNNCC 5 cut(s) 257, 258, 532, 2274, 2573
NmeAIII GCCGAG 2 cut(s) 167, 1231
NmuCI GTSAC 3 cut(s) 603, 1238, 2360
NotI GCGGCCGC 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 454
OliI CACNNNNGTG 1 cut(s) 1124
PagI TCATGA 2 cut(s) 1195, 2581
PasI CCCWGGG 1 cut(s) 89
PauI GCGCGC 1 cut(s) 268
PcsI WCGNNNNNNNCGW 1 cut(s) 3004
PctI GAATGC 1 cut(s) 2735
PdmI GAANNNNTTC 3 cut(s) 813, 1386, 2994
PfeI GAWTC 7 cut(s) 1042, 1079, 1469, 1710, 2165, 2429, 2995
PflMI CCANNNNNTGG 3 cut(s) 1180, 2282, 2656
PfoI TCCNGGA 1 cut(s) 1448
PleI GAGTC 2 cut(s) 591, 2858
PpsI GAGTC 2 cut(s) 591, 2858
PpuMI RGGWCCY 2 cut(s) 256, 2415
PshBI ATTAAT 1 cut(s) 1271
Psp5II RGGWCCY 2 cut(s) 256, 2415
Psp6I CCWGG 6 cut(s) 88, 1345, 1448, 1999, 2618, 3049
PspEI GGTNACC 1 cut(s) 2954
PspFI CCCAGC 4 cut(s) 1665, 2520, 2684, 2789
PspGI CCWGG 6 cut(s) 88, 1345, 1448, 1999, 2618, 3049
PspN4I GGNNCC 5 cut(s) 257, 258, 532, 2274, 2573
PspPI GGNCC 5 cut(s) 149, 256, 629, 2415, 2674
PspPPI RGGWCCY 2 cut(s) 256, 2415
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 9 cut(s) 93, 511, 939, 1068, 1261, 1480, 1720, 2272, 2571
PteI GCGCGC 1 cut(s) 268
PvuII CAGCTG 1 cut(s) 2345
RsaI GTAC 1 cut(s) 3123
RsaNI GTAC 1 cut(s) 3122
RseI CAYNNNNRTG 2 cut(s) 1124, 2942
SacII CCGCGG 2 cut(s) 175, 235
Sau96I GGNCC 5 cut(s) 149, 256, 629, 2415, 2674
SchI GAGTC 2 cut(s) 592, 2858
ScrFI CCNGG 9 cut(s) 90, 260, 506, 1347, 1450, 2001, 2620, 2929, 3051
SduI GDGCHC 1 cut(s) 2691
SfcI CTRYAG 1 cut(s) 558
Sfr303I CCGCGG 2 cut(s) 175, 235
SgrBI CCGCGG 2 cut(s) 175, 235
SinI GGWCC 3 cut(s) 256, 2415, 2674
SmiMI CAYNNNNRTG 2 cut(s) 1124, 2942
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
SspI AATATT 2 cut(s) 2599, 3128
SspMI CTAG 5 cut(s) 669, 929, 1379, 2919, 2987
StyD4I CCNGG 9 cut(s) 88, 258, 504, 1345, 1448, 1999, 2618, 2927, 3049
StyI CCWWGG 4 cut(s) 19, 130, 526, 1714
TaaI ACNGT 4 cut(s) 1057, 1107, 1140, 1768
TaiI ACGT 2 cut(s) 949, 1866
TaqI TCGA 4 cut(s) 98, 805, 1135, 3018
TatI WGTACW 1 cut(s) 3121
TauI GCSGC 9 cut(s) 65, 150, 171, 174, 189, 192, 231, 234, 237
TfiI GAWTC 7 cut(s) 1042, 1079, 1469, 1710, 2165, 2429, 2995
TscAI CASTG 4 cut(s) 12, 1126, 2443, 2714
TseFI GTSAC 3 cut(s) 603, 1238, 2360
TseI GCWGC 7 cut(s) 36, 286, 1013, 2342, 2345, 2405, 3106
Tsp45I GTSAC 3 cut(s) 603, 1238, 2360
TspGWI ACGGA 3 cut(s) 335, 369, 1061
TspRI CASTG 4 cut(s) 12, 1126, 2443, 2714
Van91I CCANNNNNTGG 3 cut(s) 1180, 2282, 2656
Vha464I CTTAAG 1 cut(s) 746
VpaK11BI GGWCC 3 cut(s) 256, 2415, 2674
VspI ATTAAT 1 cut(s) 1271
XagI CCTNNNNNAGG 1 cut(s) 1030
XapI RAATTY 5 cut(s) 408, 1191, 1324, 1444, 2872
XbaI TCTAGA 3 cut(s) 928, 1378, 2986
XcmI CCANNNNNNNNNTGG 1 cut(s) 1732
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 3 cut(s) 813, 1386, 2994
XspI CTAG 5 cut(s) 669, 929, 1379, 2919, 2987
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.