pycom12g05720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010582
Physical Location & Seq
Reverse (-)
5666230 .. 5667036
807 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05720.2

Sequence Viewer

Length: 426 bp
ATGTACTCTCCCTACGTGTTTGCTAAAGTCTGTGTTTTTGATCAACTTATCCTCAATTTCACAATATTCATCATGGAAATGAAGAAGATCTCCCTCGCCATCATTGTCCTTGCTGCCTTATTGAGCGTTGTCTTGGCTGTTGACCCAGTCCCCGCTACTCCCAAGGACCCAGCTGCCGCTGCTACTAAGGACCCAGCTGCCGCTGCTCCTAAGGAATCAGCTGCAGCTACTCCTAAGGCCTCAGCTACTGCCCCCGTAGGCTCAGCTACCTCCCCCGTAGGCTCAGCTACTACCACCACAAGCACAGCATCCTCCCCTGACTCAGCTCATGCTCCTGCTCCATCAGGCACCGCCAGCACCATGCCCGTTGTCGGCTCTCTGGCCAGTGCCTTCCTCGTGACCTTCTTTGGCTATTACCTGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

14.18

Weight (kDa)

6.52

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 347
AciI CCGC 4 cut(s) 153, 177, 201, 351
AcoI YGGCCR 1 cut(s) 381
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 371
AflIII ACRYGT 1 cut(s) 15
AluBI AGCT 8 cut(s) 173, 197, 221, 227, 245, 266, 287, 326
AluI AGCT 8 cut(s) 173, 197, 221, 227, 245, 266, 287, 326
AlwNI CAGNNNCTG 1 cut(s) 248
AoxI GGCC 2 cut(s) 237, 381
ApeKI GCWGC 7 cut(s) 113, 173, 179, 197, 203, 221, 224
AspS9I GGNCC 2 cut(s) 166, 190
AvaII GGWCC 2 cut(s) 166, 190
AxyI CCTNAGG 2 cut(s) 210, 234
BalI TGGCCA 1 cut(s) 383
BanI GGYRCC 1 cut(s) 347
BauI CACGAG 1 cut(s) 395
BbvCI CCTCAGC 1 cut(s) 241
BbvI GCAGC 7 cut(s) 100, 160, 166, 184, 190, 208, 236
BccI CCATC 2 cut(s) 107, 349
BclI TGATCA 1 cut(s) 40
BfmI CTRYAG 2 cut(s) 222, 419
BglII AGATCT 1 cut(s) 87
BisI GCNGC 9 cut(s) 114, 174, 177, 180, 198, 201, 204, 222, 225
BlpI GCTNAGC 2 cut(s) 262, 283
BlsI GCNGC 9 cut(s) 115, 175, 178, 181, 199, 202, 205, 223, 226
Bme18I GGWCC 2 cut(s) 166, 190
BmgT120I GGNCC 2 cut(s) 166, 190
BmiI GGNNCC 3 cut(s) 168, 192, 349
BmrI ACTGGG 1 cut(s) 140
BmsI GCATC 1 cut(s) 317
BmuI ACTGGG 1 cut(s) 140
Bpu10I CCTNAGC 1 cut(s) 241
Bpu1102I GCTNAGC 2 cut(s) 262, 283
BsaAI YACGTR 1 cut(s) 16
BsaJI CCNNGG 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse1I ACTGG 2 cut(s) 146, 384
Bse21I CCTNAGG 2 cut(s) 210, 234
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 308
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMII CTCAG 4 cut(s) 255, 276, 297, 336
BseNI ACTGG 2 cut(s) 146, 384
BseXI GCAGC 7 cut(s) 100, 160, 166, 184, 190, 208, 236
BseYI CCCAGC 2 cut(s) 169, 193
BshFI GGCC 2 cut(s) 239, 383
BshNI GGYRCC 1 cut(s) 347
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 2 cut(s) 239, 383
Bsp143I GATC 2 cut(s) 40, 87
Bsp1720I GCTNAGC 2 cut(s) 262, 283
BspACI CCGC 4 cut(s) 153, 177, 201, 351
BspANI GGCC 2 cut(s) 239, 383
BspCNI CTCAG 4 cut(s) 254, 275, 296, 335
BspLI GGNNCC 3 cut(s) 168, 192, 349
BspMAI CTGCAG 2 cut(s) 226, 423
BspT107I GGYRCC 1 cut(s) 347
BsrI ACTGG 2 cut(s) 146, 384
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 2 cut(s) 40, 87
BssSI CACGAG 1 cut(s) 395
BssT1I CCWWGG 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 395
BstBAI YACGTR 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 7 cut(s) 186, 210, 234, 241, 262, 283, 322
BstF5I GGATG 1 cut(s) 308
BstKTI GATC 2 cut(s) 43, 90
BstMBI GATC 2 cut(s) 40, 87
BstMWI GCNNNNNNNGC 3 cut(s) 179, 203, 354
BstSFI CTRYAG 2 cut(s) 222, 419
BstV1I GCAGC 7 cut(s) 100, 160, 166, 184, 190, 208, 236
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
Bsu36I CCTNAGG 2 cut(s) 210, 234
BsuRI GGCC 2 cut(s) 239, 383
BtsCI GGATG 1 cut(s) 308
BtsIMutI CAGTG 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 355
CaiI CAGNNNCTG 1 cut(s) 248
Cfr13I GGNCC 2 cut(s) 166, 190
Csp6I GTAC 1 cut(s) 4
CviAII CATG 3 cut(s) 73, 329, 361
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 7 cut(s) 186, 210, 234, 241, 262, 283, 322
DpnI GATC 2 cut(s) 42, 89
DpnII GATC 2 cut(s) 40, 87
EaeI YGGCCR 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 162
Eco147I AGGCCT 1 cut(s) 239
Eco47I GGWCC 2 cut(s) 166, 190
Eco81I CCTNAGG 2 cut(s) 210, 234
EcoO109I RGGNCCY 2 cut(s) 166, 190
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 3 cut(s) 76, 332, 364
FaiI YATR 3 cut(s) 74, 330, 362
FaqI GGGAC 1 cut(s) 134
FatI CATG 3 cut(s) 72, 328, 360
FauI CCCGC 1 cut(s) 160
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 9 cut(s) 114, 174, 177, 180, 198, 201, 204, 222, 225
FokI GGATG 1 cut(s) 295
Fsp4HI GCNGC 9 cut(s) 114, 174, 177, 180, 198, 201, 204, 222, 225
GluI GCNGC 9 cut(s) 114, 174, 177, 180, 198, 201, 204, 222, 225
GsaI CCCAGC 2 cut(s) 173, 197
HaeIII GGCC 2 cut(s) 239, 383
Hin1II CATG 3 cut(s) 76, 332, 364
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 2 cut(s) 215, 320
Hpy166II GTNNAC 1 cut(s) 142
Hpy188III TCNNGA 1 cut(s) 397
Hpy8I GTNNAC 1 cut(s) 142
HpyAV CCTTC 2 cut(s) 400, 412
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 2 cut(s) 224, 421
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 203, 354
HpyF3I CTNAG 7 cut(s) 186, 210, 234, 241, 262, 283, 322
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 3 cut(s) 76, 332, 364
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 2 cut(s) 40, 87
LmnI GCTCC 3 cut(s) 211, 337, 343
LpnPI CCDG 9 cut(s) 159, 183, 207, 330, 330, 348, 365, 367, 397
Lsp1109I GCAGC 7 cut(s) 100, 160, 166, 184, 190, 208, 236
LweI GCATC 1 cut(s) 317
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 397
MalI GATC 2 cut(s) 42, 89
MboI GATC 2 cut(s) 40, 87
MboII GAAGA 2 cut(s) 94, 97
MflI RGATCY 1 cut(s) 87
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 1 cut(s) 55
MluNI TGGCCA 1 cut(s) 383
MlyI GAGTC 1 cut(s) 314
MnlI CCTC 6 cut(s) 62, 104, 250, 280, 322, 404
Mox20I TGGCCA 1 cut(s) 383
MscI TGGCCA 1 cut(s) 383
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 383
MspA1I CMGCKG 5 cut(s) 173, 179, 197, 203, 221
MwoI GCNNNNNNNGC 3 cut(s) 179, 203, 354
NdeII GATC 2 cut(s) 40, 87
NlaIII CATG 3 cut(s) 76, 332, 364
NlaIV GGNNCC 3 cut(s) 168, 192, 349
NmuCI GTSAC 1 cut(s) 397
PceI AGGCCT 1 cut(s) 239
PfeI GAWTC 1 cut(s) 215
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 9 cut(s) 115, 175, 178, 181, 199, 202, 205, 223, 226
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
Ppu21I YACGTR 1 cut(s) 16
PpuMI RGGWCCY 2 cut(s) 166, 190
Psp5II RGGWCCY 2 cut(s) 166, 190
PspFI CCCAGC 2 cut(s) 169, 193
PspN4I GGNNCC 3 cut(s) 168, 192, 349
PspPI GGNCC 2 cut(s) 166, 190
PspPPI RGGWCCY 2 cut(s) 166, 190
PstI CTGCAG 2 cut(s) 226, 423
PstNI CAGNNNCTG 1 cut(s) 248
PsuI RGATCY 1 cut(s) 87
PsyI GACNNNGTC 1 cut(s) 146
PvuII CAGCTG 3 cut(s) 173, 197, 221
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 9 cut(s) 114, 174, 177, 180, 198, 201, 204, 222, 225
Sau3AI GATC 2 cut(s) 40, 87
Sau96I GGNCC 2 cut(s) 166, 190
SchI GAGTC 1 cut(s) 314
SfaNI GCATC 1 cut(s) 317
SfcI CTRYAG 2 cut(s) 222, 419
SinI GGWCC 2 cut(s) 166, 190
SmiMI CAYNNNNRTG 1 cut(s) 77
Sse9I AATT 1 cut(s) 55
SseBI AGGCCT 1 cut(s) 239
SsiI CCGC 4 cut(s) 153, 177, 201, 351
SspI AATATT 1 cut(s) 66
StuI AGGCCT 1 cut(s) 239
StyI CCWWGG 1 cut(s) 162
TaiI ACGT 1 cut(s) 18
TasI AATT 1 cut(s) 55
TatI WGTACW 1 cut(s) 3
TauI GCSGC 2 cut(s) 179, 203
TfiI GAWTC 1 cut(s) 215
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 7 cut(s) 113, 173, 179, 197, 203, 221, 224
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 2 cut(s) 58, 95
TspRI CASTG 1 cut(s) 391
Tth111I GACNNNGTC 1 cut(s) 146
VpaK11BI GGWCC 2 cut(s) 166, 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.