pycom12g07370

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008128
Physical Location & Seq
Forward (+)
7708733 .. 7710748
2016 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1071 bp
ATGAACTTTATATATGTCTCCAATAGCTTCATGCATTTTTCCAACATATATAAAACTGGAAGAGCTCTCATGGCTTCCATATTACTACTACTTGTAGTCTTGATGCCAGCCCTGCAAATAACAGGTGCACAATCTGTTGGTGTTTGTTATGGACGAAACGGCAACAATTTACCAGCTGAAGGAGAAGTGGTCGACTTGTACAAAAGCAATGGCATCGGACGGATGAGGATCTATGAACCAAATGAAGCAACCTTGCAAGCCCTTAGGGGTTCCAACATAGAACTCACAATCACCATCCTCAACAGCGAGCTTCCAGCCCTCAATGATGCAACTGCTGCAACTGCCTGGGTCCAGAAGAACGTACAACCCTATTCAGCTGATGTTCGATTCAAATACATCGCTGTTGGAAACGAAATACACCGCGATTCTGCAGAGGTTGGGTCTCTCCTGCCTGCCATCAAAAACATCCACAGTGCAATTGTAGCAGCCAATCTGCAAGGCCAAATCAAAGTCTCTACAGCAATTGACACAACTCTTGTGGCCAATCCCTTCCCTCCCTCCGATGGAGTATATGACGCTGCAAATCAATTCATACAACCAGTCATCGACTTCCTCGTCAGCAGTGGGGCCCCACTCCTTGTGAATGTGTACCCTTACTTCAGCTACACTGATAACCCTAACATAGACCTTGCCTATGCCTTATTCACTTCGCAAGGGGTTGTAGTGCCGGATGGGACTCGATACCCTAGCCTCTTTGATGCTCTTCTGGACGCTCAGTACGCAGCTCTTGAAAAAGCTGGTGCACCAAATATGGAGATAGTCGTATCGGAGAGCGGTTGGCCTTCTGAAGGTGGTGATCGATCAACCCCTGAGAACGCAGCCACATTCTACCAGAGATCGATCAACCATGTGACGAGTACTACTGGGACTCCAAAGAGGCGTGGTAAAGCTATAGAGACTTATCTTTTTGCAATGTTTGATGAAAATCTCAAGGACGGTAAACCAGTTGAGAAACACTTTGGAGTTTTCTCCCCTAACAAACAACCCAAGTACCAACTCACATTTGGATAG

Protein Analysis

357

Amino Acids

38.73

Weight (kDa)

5.44

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 46 - 355 7.7e-105 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 614
AccBSI CCGCTC 1 cut(s) 834
AccI GTMKAC 1 cut(s) 192
AccII CGCG 1 cut(s) 423
AciI CCGC 2 cut(s) 421, 834
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 540
AcuI CTGAAG 3 cut(s) 198, 643, 867
AfaI GTAC 6 cut(s) 200, 363, 650, 779, 919, 1052
AfiI CCNNNNNNNGG 3 cut(s) 179, 563, 848
AgsI TTSAA 2 cut(s) 391, 791
AjnI CCWGG 1 cut(s) 344
AluBI AGCT 9 cut(s) 27, 65, 176, 310, 377, 663, 785, 797, 950
AluI AGCT 9 cut(s) 27, 65, 176, 310, 377, 663, 785, 797, 950
Alw21I GWGCWC 3 cut(s) 67, 130, 805
Alw26I GTCTC 4 cut(s) 22, 447, 517, 950
Alw44I GTGCAC 2 cut(s) 126, 801
AlwI GGATC 1 cut(s) 236
AoxI GGCC 4 cut(s) 499, 540, 627, 839
ApaI GGGCCC 1 cut(s) 631
ApaLI GTGCAC 2 cut(s) 126, 801
ApeKI GCWGC 5 cut(s) 335, 485, 578, 782, 878
AspS9I GGNCC 3 cut(s) 349, 627, 628
AsuHPI GGTGA 2 cut(s) 283, 866
AvaII GGWCC 1 cut(s) 349
AxyI CCTNAGG 1 cut(s) 263
BaeGI GKGCMC 3 cut(s) 130, 631, 805
BaeI ACNNNNGTAYC 2 cut(s) 1034, 1067
BalI TGGCCA 1 cut(s) 542
BanII GRGCYC 2 cut(s) 67, 631
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
Bbv12I GWGCWC 3 cut(s) 67, 130, 805
BbvI GCAGC 5 cut(s) 322, 497, 565, 794, 890
BccI CCATC 4 cut(s) 302, 464, 557, 725
BceAI ACGGC 1 cut(s) 175
BcgI CGANNNNNNTGC 2 cut(s) 196, 230
BciT130I CCWGG 1 cut(s) 346
BcoDI GTCTC 4 cut(s) 22, 447, 517, 950
BfaI CTAG 1 cut(s) 747
BfmI CTRYAG 3 cut(s) 429, 516, 951
BisI GCNGC 5 cut(s) 336, 486, 579, 783, 879
BlsI GCNGC 5 cut(s) 337, 487, 580, 784, 880
BmcAI AGTACT 1 cut(s) 919
Bme1390I CCNGG 1 cut(s) 346
Bme18I GGWCC 1 cut(s) 349
BmgT120I GGNCC 3 cut(s) 349, 627, 628
BmiI GGNNCC 5 cut(s) 271, 350, 628, 629, 630
BmrFI CCNGG 1 cut(s) 346
BmrI ACTGGG 1 cut(s) 933
BmsI GCATC 4 cut(s) 93, 222, 316, 748
BmuI ACTGGG 1 cut(s) 933
BpuEI CTTGAG 1 cut(s) 974
Bsa29I ATCGAT 2 cut(s) 859, 899
BsaBI GATNNNNATC 2 cut(s) 227, 984
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 345
BsaXI ACNNNNNCTCC 4 cut(s) 174, 204, 913, 943
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 563, 848
Bse1I ACTGG 4 cut(s) 61, 599, 928, 1004
Bse21I CCTNAGG 1 cut(s) 263
Bse3DI GCAATG 2 cut(s) 214, 978
Bse8I GATNNNNATC 2 cut(s) 227, 984
BseBI CCWGG 1 cut(s) 346
BseCI ATCGAT 2 cut(s) 859, 899
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 4 cut(s) 228, 294, 465, 736
BseJI GATNNNNATC 2 cut(s) 227, 984
BseLI CCNNNNNNNGG 3 cut(s) 179, 563, 848
BseMI GCAATG 2 cut(s) 214, 978
BseMII CTCAG 2 cut(s) 788, 861
BseNI ACTGG 4 cut(s) 61, 599, 928, 1004
BseSI GKGCMC 3 cut(s) 130, 631, 805
BseXI GCAGC 5 cut(s) 322, 497, 565, 794, 890
Bsh1236I CGCG 1 cut(s) 423
BshFI GGCC 4 cut(s) 501, 542, 629, 841
BshVI ATCGAT 2 cut(s) 859, 899
BsiHKAI GWGCWC 3 cut(s) 67, 130, 805
BsiSI CCGG 1 cut(s) 728
BslFI GGGAC 2 cut(s) 748, 940
BslI CCNNNNNNNGG 3 cut(s) 179, 563, 848
BsmAI GTCTC 4 cut(s) 22, 447, 517, 950
BsmFI GGGAC 2 cut(s) 748, 940
BsnI GGCC 4 cut(s) 501, 542, 629, 841
Bso31I GGTCTC 1 cut(s) 447
Bsp120I GGGCCC 1 cut(s) 627
Bsp1286I GDGCHC 4 cut(s) 67, 130, 631, 805
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 5 cut(s) 228, 856, 860, 896, 900
BspACI CCGC 2 cut(s) 421, 834
BspANI GGCC 4 cut(s) 501, 542, 629, 841
BspCNI CTCAG 2 cut(s) 787, 862
BspDI ATCGAT 2 cut(s) 859, 899
BspFNI CGCG 1 cut(s) 423
BspLI GGNNCC 5 cut(s) 271, 350, 628, 629, 630
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 1 cut(s) 236
BspQI GCTCTTC 2 cut(s) 55, 768
BspTNI GGTCTC 1 cut(s) 447
BsrBI CCGCTC 1 cut(s) 834
BsrDI GCAATG 2 cut(s) 214, 978
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 4 cut(s) 61, 599, 928, 1004
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 5 cut(s) 228, 856, 860, 896, 900
Bst2UI CCWGG 1 cut(s) 346
Bst4CI ACNGT 2 cut(s) 473, 998
Bst6I CTCTTC 2 cut(s) 55, 768
BstAPI GCANNNNNTGC 1 cut(s) 335
BstAUI TGTACA 1 cut(s) 198
BstC8I GCNNGC 4 cut(s) 108, 258, 308, 453
BstDEI CTNAG 3 cut(s) 263, 774, 870
BstENI CCTNNNNNAGG 1 cut(s) 846
BstF5I GGATG 4 cut(s) 228, 294, 465, 736
BstFNI CGCG 1 cut(s) 423
BstKTI GATC 5 cut(s) 231, 859, 863, 899, 903
BstMAI GTCTC 4 cut(s) 22, 447, 517, 950
BstMBI GATC 5 cut(s) 228, 856, 860, 896, 900
BstMWI GCNNNNNNNGC 6 cut(s) 71, 112, 335, 341, 482, 779
BstNI CCWGG 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 344
BstSFI CTRYAG 3 cut(s) 429, 516, 951
BstSLI GKGCMC 3 cut(s) 130, 631, 805
BstUI CGCG 1 cut(s) 423
BstV1I GCAGC 5 cut(s) 322, 497, 565, 794, 890
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
Bsu15I ATCGAT 2 cut(s) 859, 899
Bsu36I CCTNAGG 1 cut(s) 263
BsuRI GGCC 4 cut(s) 501, 542, 629, 841
BsuTUI ATCGAT 2 cut(s) 859, 899
BtgZI GCGATG 1 cut(s) 382
BtsCI GGATG 4 cut(s) 228, 294, 465, 736
BtsI GCAGTG 1 cut(s) 628
BtsIMutI CAGTG 3 cut(s) 478, 628, 666
Cac8I GCNNGC 4 cut(s) 108, 258, 308, 453
Cfr13I GGNCC 3 cut(s) 349, 627, 628
ClaI ATCGAT 2 cut(s) 859, 899
CseI GACGC 2 cut(s) 584, 779
Csp6I GTAC 6 cut(s) 199, 362, 649, 778, 918, 1051
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviAII CATG 3 cut(s) 31, 70, 908
CviQI GTAC 6 cut(s) 199, 362, 649, 778, 918, 1051
DdeI CTNAG 3 cut(s) 263, 774, 870
DpnI GATC 5 cut(s) 230, 858, 862, 898, 902
DpnII GATC 5 cut(s) 228, 856, 860, 896, 900
DrdI GACNNNNNNGTC 1 cut(s) 614
DseDI GACNNNNNNGTC 1 cut(s) 614
EaeI YGGCCR 1 cut(s) 540
Eam1104I CTCTTC 2 cut(s) 55, 768
EarI CTCTTC 2 cut(s) 55, 768
Ecl136II GAGCTC 1 cut(s) 65
Eco24I GRGCYC 2 cut(s) 67, 631
Eco31I GGTCTC 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 65
Eco57I CTGAAG 3 cut(s) 198, 643, 867
Eco81I CCTNAGG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 65
EcoNI CCTNNNNNAGG 1 cut(s) 846
EcoO109I RGGNCCY 2 cut(s) 627, 628
EcoRII CCWGG 1 cut(s) 344
EcoT22I ATGCAT 1 cut(s) 36
EcoT38I GRGCYC 2 cut(s) 67, 631
FaeI CATG 3 cut(s) 34, 73, 911
FaqI GGGAC 2 cut(s) 748, 940
FatI CATG 3 cut(s) 30, 69, 907
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 5 cut(s) 336, 486, 579, 783, 879
FokI GGATG 4 cut(s) 235, 281, 452, 743
FriOI GRGCYC 2 cut(s) 67, 631
Fsp4HI GCNGC 5 cut(s) 336, 486, 579, 783, 879
FspBI CTAG 1 cut(s) 747
GluI GCNGC 5 cut(s) 336, 486, 579, 783, 879
HaeIII GGCC 4 cut(s) 501, 542, 629, 841
HapII CCGG 1 cut(s) 728
HgaI GACGC 2 cut(s) 584, 779
Hin1II CATG 3 cut(s) 34, 73, 911
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 4 cut(s) 387, 425, 736, 928
HpaII CCGG 1 cut(s) 728
HphI GGTGA 2 cut(s) 283, 866
Hpy166II GTNNAC 5 cut(s) 128, 193, 649, 803, 1001
Hpy188I TCNGA 4 cut(s) 218, 562, 829, 847
Hpy188III TCNNGA 4 cut(s) 100, 352, 767, 788
Hpy8I GTNNAC 5 cut(s) 128, 193, 649, 803, 1001
HpyAV CCTTC 4 cut(s) 173, 559, 842, 852
HpyCH4III ACNGT 2 cut(s) 473, 998
HpyCH4IV ACGT 1 cut(s) 360
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 112, 335, 341, 482, 779
HpyF3I CTNAG 3 cut(s) 263, 774, 870
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 3 cut(s) 34, 73, 911
Kzo9I GATC 5 cut(s) 228, 856, 860, 896, 900
LguI GCTCTTC 2 cut(s) 55, 768
Lsp1109I GCAGC 5 cut(s) 322, 497, 565, 794, 890
LweI GCATC 4 cut(s) 93, 222, 316, 748
MaeI CTAG 1 cut(s) 747
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 910
MalI GATC 5 cut(s) 230, 858, 862, 898, 902
MbiI CCGCTC 1 cut(s) 834
MboI GATC 5 cut(s) 228, 856, 860, 896, 900
MboII GAAGA 3 cut(s) 72, 367, 755
MfeI CAATTG 2 cut(s) 477, 522
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 4 cut(s) 67, 130, 631, 805
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 4 cut(s) 166, 477, 522, 587
MluNI TGGCCA 1 cut(s) 542
MlyI GAGTC 2 cut(s) 730, 922
MmeI TCCRAC 3 cut(s) 66, 297, 385
MnlI CCTC 9 cut(s) 219, 308, 329, 427, 564, 568, 623, 761, 930
Mox20I TGGCCA 1 cut(s) 542
Mph1103I ATGCAT 1 cut(s) 36
MscI TGGCCA 1 cut(s) 542
Msp20I TGGCCA 1 cut(s) 542
MspA1I CMGCKG 2 cut(s) 176, 377
MspI CCGG 1 cut(s) 728
MspR9I CCNGG 1 cut(s) 346
MunI CAATTG 2 cut(s) 477, 522
MvaI CCWGG 1 cut(s) 346
MvnI CGCG 1 cut(s) 423
MwoI GCNNNNNNNGC 6 cut(s) 71, 112, 335, 341, 482, 779
NdeII GATC 5 cut(s) 228, 856, 860, 896, 900
NlaIII CATG 3 cut(s) 34, 73, 911
NlaIV GGNNCC 5 cut(s) 271, 350, 628, 629, 630
NmuCI GTSAC 1 cut(s) 910
NsiI ATGCAT 1 cut(s) 36
PciSI GCTCTTC 2 cut(s) 55, 768
PcsI WCGNNNNNNNCGW 1 cut(s) 612
PfeI GAWTC 2 cut(s) 387, 425
PkrI GCNGC 5 cut(s) 337, 487, 580, 784, 880
PleI GAGTC 2 cut(s) 730, 922
PpsI GAGTC 2 cut(s) 730, 922
Psp124BI GAGCTC 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 344
PspGI CCWGG 1 cut(s) 344
PspN4I GGNNCC 5 cut(s) 271, 350, 628, 629, 630
PspOMI GGGCCC 1 cut(s) 627
PspPI GGNCC 3 cut(s) 349, 627, 628
PstI CTGCAG 1 cut(s) 433
PsuI RGATCY 1 cut(s) 228
PvuII CAGCTG 2 cut(s) 176, 377
RsaI GTAC 6 cut(s) 200, 363, 650, 779, 919, 1052
RsaNI GTAC 6 cut(s) 199, 362, 649, 778, 918, 1051
SacI GAGCTC 1 cut(s) 67
SalI GTCGAC 1 cut(s) 191
SapI GCTCTTC 2 cut(s) 55, 768
SatI GCNGC 5 cut(s) 336, 486, 579, 783, 879
Sau3AI GATC 5 cut(s) 228, 856, 860, 896, 900
Sau96I GGNCC 3 cut(s) 349, 627, 628
ScaI AGTACT 1 cut(s) 919
SchI GAGTC 2 cut(s) 730, 922
ScrFI CCNGG 1 cut(s) 346
SduI GDGCHC 4 cut(s) 67, 130, 631, 805
SfaNI GCATC 4 cut(s) 93, 222, 316, 748
SfcI CTRYAG 3 cut(s) 429, 516, 951
SinI GGWCC 1 cut(s) 349
SmlI CTYRAG 1 cut(s) 989
SmoI CTYRAG 1 cut(s) 989
Sse9I AATT 4 cut(s) 166, 477, 522, 587
SsiI CCGC 2 cut(s) 421, 834
SspMI CTAG 1 cut(s) 747
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 1 cut(s) 344
TaaI ACNGT 2 cut(s) 473, 998
TaiI ACGT 1 cut(s) 363
TaqI TCGA 6 cut(s) 192, 385, 606, 739, 859, 899
TasI AATT 4 cut(s) 166, 477, 522, 587
TatI WGTACW 2 cut(s) 198, 917
TfiI GAWTC 2 cut(s) 387, 425
TscAI CASTG 3 cut(s) 478, 628, 673
TseFI GTSAC 1 cut(s) 910
TseI GCWGC 5 cut(s) 335, 485, 578, 782, 878
Tsp45I GTSAC 1 cut(s) 910
TspDTI ATGAA 6 cut(s) 17, 19, 249, 258, 580, 996
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 3 cut(s) 478, 628, 673
VneI GTGCAC 2 cut(s) 126, 801
VpaK11BI GGWCC 1 cut(s) 349
XagI CCTNNNNNAGG 1 cut(s) 846
XcmI CCANNNNNNNNNTGG 1 cut(s) 1061
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 1 cut(s) 747
ZrmI AGTACT 1 cut(s) 919
Zsp2I ATGCAT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.