pycom12g16760

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004862
Physical Location & Seq
Forward (+)
19064348 .. 19069613
5266 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4137 bp
ATGTTGATCCTGGACATATGTCGAGATCGGGGCGTGACACAATCTCTCTCTCTCTCCCTCTCTCTCCCCTTCCTCTCTTCCTCCAAATCACTCTCTCCAGTCTTCTCCTGTCCTCCTCGCGGATTCCTTCTTCCAAACCATGATTTTCCCTCCCCCCCCTTTGGAATCAGAACCCCTCCGAATTGGGAGATTCTAATCCGGGTTTTCCAATCTCTCACAGGTAATTTTCGAATCAAAACGAATATGATTTATATTGTGATTTTCTGTATTAAAATGGGTTTCTCTTTTAATTATTTTGTTGGGGTTTTGCATATTATAATCTTCCAATTTGGCAGCAAATGCATAAGTGTTGTAAAAATCTCTCTTGGCTGCTATTTAGTTATATTGGGTTTACTTTCATGGAAAAATCAAAGATTTAACACATGCATGCGTTTTCTCTACGAGAGCTCTCAGATCTGCAACAAGAAAGACGCACCGACACCTAATAATGTCAGATCCATGGCTAATCGTCCCAATTCGTATTCATTTGATGCAGAAGTTTCTTCATGGAAAAAACTGAAAAATAAATCGAAACAGAACCGAAAAAAAATTGAACCAAACCAAACTAAACCAAAATTTCTATTCGGTTTCAATTTTGGCAAAAAATGTAACCGTTTCAAACCGAACCCAGCCCTAGGACTGTGTAACCAAGTTGGACTTATACCCTTGGTCACATTGAGGGATTTTAGAGTGCATTTTTGCTCACCACCATTTAGTGTGGTTTTGAGATTTGTACCTATTTACTACACGAATCTCGAAATTCAAATTCATCTAACGATGATAAATAGGATGGTGAACATAAGAGCAAAAATTAAGGTTATATTTCTTATGATGAAAGGTAATTACGTTGATAAGACAGGAATCATTATCTATCTAATTTTCTTGATTAAAATACAAAGGATACGTGTGACGTATAAGTCACCACCTCAAGAAGCAGCCAAAACAGCAAAGAGAATTGAGTTGGGAACTATTTTCTCCATTTCAATAATCATCTGTTTTTTGTTAACTATTTTATGCTTAATTAATTTGTCATTACGTTGTCACCAACCGATATATAATGATGGATTAAAATTCATCAATTCACCAATAAAGAAAAAGATGATGCAATCAGAGATGAAGTTTCAGACCTTCTGGCCATGCCTGAATGAAACAAATTCAACTTTGGATACAAATGAATACACTGAGCAGATGGGGAGCTCTGAATTTTCTTCGATTTTCATGCCATCGGACGAATTTTCAGAAACTACTAGTTCTTTTCCATTTTCAACCATGTTTTCTGGTGAATTTGAACAACTTGCAGATTGTGATCATCTCCTGCAAGCCACATGGATGGCGGAAGAGGAATTTCCCATGGAGTTGGGAGGATTCGAGACGAATTATTTGATGGGTGAGATAGAAAATATGTATACCTCAGATTGTGATCAGCTTCCTTCACCGCAATTTTTTGTTGAAGGAAATGGTGTCTGGAGTCCAAACTCTTTTGTGACATCTGAGGCATCCTCCATGGATGTGACATCCGTCCAACAATCAATAACCCTGCCGAGAGACGAGATGGAAATCAACAACGAAGTGAGAATTCGCCATATGCTCAAGGCGTTCGGCGAGGCCATGGAGATGGGGCAGAGGAAGCTGGAGGAGGTAATCATGAGATGCCTTTTGGAGAAAGTAAACCCACTTGGTCAACCTCTTGAACCTCTTGAGCGCCTTGCCTTCAACTTATGTCAAGGAGTTGTTGATAATCAGCAGGGAGACTATTTGAAACAAGAATCTTGCAAGAATTACGAGGCTGCATTCAACTTGTTCTACCAAATCTTCCCTTTTGGGAGATTTGCTCACTACGCAGCAAACACCGGATCGATCCTCGAGGCTGTCCCAGAGGATGTAGAGACTGTTCATGTAGTGGAATTTGATATCGGTGAAGGGGTTCAACTGTCCCAGATGATTGAGGCTGTGGCAAGGAGAAACAAGACACTTAAAGTGACAGCAATTAAATGGGATGTGGAAGAGAGTGATGAGGTTGCTCCTCCACATTGGAGATTTGAGGAGACGAAAAGGCAACTCCAGCATCACGCGAGGTCTTTCGGGGTAAACTTGAAGGTGGAAGAGATCGCGATCGAGGATTTGGTGAGTCTAATCAAGAAGGCCAACAAGAGAGATGGAAGGAGAGAATTTCTAGCCTTCAACTCTATGGTAGGGCTTCCTCACATGAGGAGGAAAAGAAGCAGAGGACTTCTCATGGAATTCCTAAGGTTAGCTAAGGATTTGTTAGCCAATTCGGCAAGAGGTATCATAACCTTTGGTGACGGAGATGCTTGCGCGAAAAGGGAAAATGACTCGGGTTTCAGCTCCTTCTTTGACGCGAATGTTGTGCACTACGAAGCCTTGTTAGGGTCTTTGGAATCGAGCTTCCCAAGCCATCTAGGAGAGGCAAGGATGGTGATGGAATTGATGTTTGTGGCGCCGTATGTGTCTTCTCAGGCTTGGTTCGAGAAGTGGAACGAAGCAAGAGGTGGAAATCTTCATCCCTGGTTCGGGCTGGAGGGTAGGAGAGTGAGCAGGAAAATGTTAATGGAGGCCAAAGAAATTGTGGGAGCAGATAGCTTGTTTGGAGTGAGAGTTGGAGGAGAGAATGGGAATGAGATGACTTTGGAATGGAATTCAATTCCTTTGATCAGAATTTTTTTTACTCTCTTTTCAAATTATTGCAGCAAATGTGTAGACTGCTGGAGATTTACCATAAGAAGTTTTTTGTACAGATTCTTTAAATTTCATGTTGTAAAAAGAAGATCAACTAAGTTGACAACTAATGAAAATAGTTTGAAAATTTTGAGTCTTAACAATAAGGACAAAATAAAGAGTAAAATGAATAGTACTAGGATTGACTTTTTAGTCTTTTCGTTAAAGTTCCCTTATTATAAAATCTCATTTTTGAACATTGTAATGTCATACATTAATTTACGAATTCGCTGCAATGTTAGACCTCCATTAATAACTCATCAAATTAGTTATGGAAGTCAATTGTACTATGTTGATTACAAAATATGGGCCTCCAGTCAATTCACTAATCCACCGTCTACATTACTCACAGACGGCAATCTCGTTGTCACTCTCTTCTCTGGAACAGGAACCAGAAATTTTTATTTATTTTATTTTTGTGTTTCAAACGGTTACCCGATGAAATTTTCATCGAGTCCAATCATTGCACAAGATATTTTCGTTGGGTCAAGGTGGGAAGCGCACCTTTTACCAAACATTTTTTTTCGTCGGCCAAACTCAATTTTGTTGACAAATGACTTTTTTTCATTGGATAAGACTTCGAGTGACGACTTTTGCGCGACCACTTTTTTATACGAATGTACGTCGCCTAAAGTTTCAGACGACAAAACCCCATTTGACCCCACAGAAAAATTTTCATCTATCAATTTTTTATTTTTATTTTTAGTGATGAGAATTTATGAGACAAAAACTTGTTCTAAACCTAATATTTTACTAAGCATCAGTTTTGTCCTTCTAAGAGCTGGACCAAGAACATGTACTCTAAACTTGTTTTATCTGTTGACTGATTATATGGCTCGTTCTTTGATTGAATGCCAGAGATTAGTATCTTCTGATCAGCAAACATTATTTCCCGGCATCATGATTTATTTCTATCTAGTCAATTTGATGCTTAATTATTTGGCATATATACACTTTTGTTTCGGCGGCCAACTAATAGCTAGGATTATCGAGGTCGGATTTAAACCACTTGTCTTAATTAGACTTAATGCACAGTTAGGACTGTATTGCAGCATCATAATCTTCTTAAAATCCATGAGGGATTTTTGGAGATATTTAATATTAAAAAATGACTATGTTGTTACCAAAAAATATATCTCTTTCAGAAATAATGTAAGCAAACACTCATTGTTTTTAATTGGTTTTTTGAATTTCTACAAAAACAATAACTTATTTTGCGACATTTTAGAGATGTTGGGCCAACAAAATGACGGGCAGTACAAATTCTTTTTACTAATTTTTTTGTTTTTGTTTTTTAATAAATCTGAGTTGTTAAAATTTGGCCTTGATCTAAGGTTTTGTGAGAATAAATATATAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

1379

Amino Acids

159.14

Weight (kDa)

9.01

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 540 - 904 1.2e-30 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 317, 2955
AasI GACNNNNNNGTC 2 cut(s) 955, 2927
AbsI CCTCGAGG 1 cut(s) 1901
AccB1I GGYRCC 1 cut(s) 2497
AccI GTMKAC 3 cut(s) 1445, 2757, 3113
AccII CGCG 6 cut(s) 120, 2111, 2150, 2357, 2399, 3374
AciI CCGC 4 cut(s) 120, 1373, 1475, 3741
AclWI GGATC 3 cut(s) 489, 1891, 1900
AcoI YGGCCR 3 cut(s) 1172, 3305, 3742
AcyI GRCGYC 1 cut(s) 2498
AfaI GTAC 7 cut(s) 774, 2792, 2911, 3062, 3397, 3574, 4034
AfiI CCNNNNNNNGG 6 cut(s) 119, 161, 674, 2427, 2570, 2571
AflIII ACRYGT 2 cut(s) 943, 3569
AhlI ACTAGT 1 cut(s) 1286
AjnI CCWGG 2 cut(s) 9, 2564
Alw21I GWGCWC 3 cut(s) 449, 1238, 2412
Alw26I GTCTC 6 cut(s) 1403, 1578, 1782, 1919, 2078, 3491
Alw44I GTGCAC 1 cut(s) 2408
AlwI GGATC 3 cut(s) 489, 1891, 1900
Ama87I CYCGRG 2 cut(s) 1901, 2374
AoxI GGCC 9 cut(s) 1172, 1644, 2181, 2613, 3084, 3305, 3742, 4012, 4096
ApaLI GTGCAC 1 cut(s) 2408
ApeKI GCWGC 8 cut(s) 333, 369, 974, 1826, 1880, 2745, 3006, 3825
ArsI GACNNNNNNTTYG 2 cut(s) 1402, 1434
AseI ATTAAT 3 cut(s) 1062, 2991, 3026
Asp700I GAANNNNTTC 1 cut(s) 1962
AspA2I CCTAGG 1 cut(s) 673
AspLEI GCGC 5 cut(s) 1743, 2357, 2500, 3277, 3374
AspS9I GGNCC 3 cut(s) 3084, 3560, 4012
AsuC2I CCSGG 2 cut(s) 200, 3669
AsuII TTCGAA 1 cut(s) 229
AvaI CYCGRG 2 cut(s) 1901, 2374
AvaII GGWCC 1 cut(s) 3560
AvrII CCTAGG 1 cut(s) 673
AxyI CCTNAGG 1 cut(s) 2285
BaeGI GKGCMC 1 cut(s) 2412
BalI TGGCCA 1 cut(s) 1174
BanI GGYRCC 1 cut(s) 2497
BanII GRGCYC 2 cut(s) 449, 1238
BbsI GAAGAC 2 cut(s) 94, 2502
Bbv12I GWGCWC 3 cut(s) 449, 1238, 2412
BbvI GCAGC 8 cut(s) 345, 356, 986, 1813, 1892, 2757, 2993, 3837
BceAI ACGGC 2 cut(s) 2485, 3145
BcgI CGANNNNNNTGC 6 cut(s) 1240, 1274, 2389, 2423, 2988, 3022
BciT130I CCWGG 2 cut(s) 11, 2566
BciVI GTATCC 2 cut(s) 933, 1198
BclI TGATCA 4 cut(s) 1345, 1459, 2709, 3649
BcnI CCSGG 2 cut(s) 200, 3669
BcoDI GTCTC 6 cut(s) 1403, 1578, 1782, 1919, 2078, 3491
BcuI ACTAGT 1 cut(s) 1286
BfaI CTAG 7 cut(s) 674, 1287, 2213, 2459, 2913, 3692, 3756
BfoI RGCGCY 2 cut(s) 1744, 2501
BfuI GTATCC 2 cut(s) 933, 1198
BglI GCCNNNNNGGC 1 cut(s) 2315
BglII AGATCT 1 cut(s) 453
BisI GCNGC 9 cut(s) 334, 370, 975, 1827, 1881, 2746, 3007, 3742, 3826
BlnI CCTAGG 1 cut(s) 673
BlsI GCNGC 9 cut(s) 335, 371, 976, 1828, 1882, 2747, 3008, 3743, 3827
BmcAI AGTACT 1 cut(s) 2911
Bme1390I CCNGG 4 cut(s) 11, 200, 2566, 3669
Bme18I GGWCC 1 cut(s) 3560
BmeT110I CYCGRG 2 cut(s) 1901, 2374
BmgT120I GGNCC 3 cut(s) 3084, 3560, 4012
BmiI GGNNCC 2 cut(s) 2499, 3166
BmrFI CCNGG 4 cut(s) 11, 200, 2566, 3669
BoxI GACNNNNGTC 2 cut(s) 18, 1556
BpiI GAAGAC 2 cut(s) 94, 2502
BplI GAGNNNNNCTC 6 cut(s) 1523, 1555, 1855, 1887, 2256, 2288
BpmI CTGGAG 7 cut(s) 81, 1525, 1691, 2084, 2597, 2785, 3073
Bpu10I CCTNAGC 1 cut(s) 2295
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 3 cut(s) 951, 1613, 1757
BpuMI CCSGG 2 cut(s) 200, 3669
Bsa29I ATCGAT 1 cut(s) 1895
BsaAI YACGTR 1 cut(s) 944
BsaBI GATNNNNATC 6 cut(s) 194, 1344, 1458, 1595, 2150, 3640
BsaHI GRCGYC 1 cut(s) 2498
BsaJI CCNNGG 7 cut(s) 498, 673, 705, 1389, 1542, 1647, 2564
BsaWI WCCGGW 1 cut(s) 1889
BsaXI ACNNNNNCTCC 2 cut(s) 2640, 2670
Bsc4I CCNNNNNNNGG 6 cut(s) 119, 161, 674, 2427, 2570, 2571
Bse1I ACTGG 2 cut(s) 98, 3090
Bse21I CCTNAGG 1 cut(s) 2285
Bse3DI GCAATG 2 cut(s) 3016, 3237
Bse8I GATNNNNATC 6 cut(s) 194, 1344, 1458, 1595, 2150, 3640
BseBI CCWGG 2 cut(s) 11, 2566
BseCI ATCGAT 1 cut(s) 1895
BseDI CCNNGG 7 cut(s) 498, 673, 705, 1389, 1542, 1647, 2564
BseGI GGATG 9 cut(s) 834, 1374, 1535, 1552, 1553, 1924, 2041, 2478, 2560
BseJI GATNNNNATC 6 cut(s) 194, 1344, 1458, 1595, 2150, 3640
BseLI CCNNNNNNNGG 6 cut(s) 119, 161, 674, 2427, 2570, 2571
BseMI GCAATG 2 cut(s) 3016, 3237
BseMII CTCAG 6 cut(s) 464, 1212, 1464, 1521, 2528, 4071
BseNI ACTGG 2 cut(s) 98, 3090
BseRI GAGGAG 6 cut(s) 105, 1688, 2052, 2096, 2263, 2676
BseSI GKGCMC 1 cut(s) 2412
BseXI GCAGC 8 cut(s) 345, 356, 986, 1813, 1892, 2757, 2993, 3837
BseYI CCCAGC 1 cut(s) 667
Bsh1236I CGCG 6 cut(s) 120, 2111, 2150, 2357, 2399, 3374
Bsh1285I CGRYCG 1 cut(s) 2154
BshFI GGCC 9 cut(s) 1174, 1646, 2183, 2615, 3086, 3307, 3744, 4014, 4098
BshNI GGYRCC 1 cut(s) 2497
BshVI ATCGAT 1 cut(s) 1895
BsiEI CGRYCG 1 cut(s) 2154
BsiHKAI GWGCWC 3 cut(s) 449, 1238, 2412
BsiHKCI CYCGRG 2 cut(s) 1901, 2374
BsiSI CCGG 3 cut(s) 199, 1890, 3669
BslFI GGGAC 3 cut(s) 495, 1895, 1957
BslI CCNNNNNNNGG 6 cut(s) 119, 161, 674, 2427, 2570, 2571
BsmAI GTCTC 6 cut(s) 1403, 1578, 1782, 1919, 2078, 3491
BsmBI CGTCTC 3 cut(s) 1403, 1578, 2078
BsmFI GGGAC 3 cut(s) 495, 1895, 1957
BsmI GAATGC 2 cut(s) 1829, 3632
BsnI GGCC 9 cut(s) 1174, 1646, 2183, 2615, 3086, 3307, 3744, 4014, 4098
BsoBI CYCGRG 2 cut(s) 1901, 2374
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 3 cut(s) 449, 1238, 2412
Bsp1407I TGTACA 1 cut(s) 2790
Bsp19I CCATGG 4 cut(s) 498, 1389, 1542, 1647
Bsp68I TCGCGA 1 cut(s) 2150
BspACI CCGC 4 cut(s) 120, 1373, 1475, 3741
BspANI GGCC 9 cut(s) 1174, 1646, 2183, 2615, 3086, 3307, 3744, 4014, 4098
BspCNI CTCAG 6 cut(s) 463, 1213, 1463, 1522, 2527, 4072
BspDI ATCGAT 1 cut(s) 1895
BspFNI CGCG 6 cut(s) 120, 2111, 2150, 2357, 2399, 3374
BspHI TCATGA 2 cut(s) 1683, 3675
BspLI GGNNCC 2 cut(s) 2499, 3166
BspPI GGATC 3 cut(s) 489, 1891, 1900
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 2497
BsrDI GCAATG 2 cut(s) 3016, 3237
BsrGI TGTACA 1 cut(s) 2790
BsrI ACTGG 2 cut(s) 98, 3090
BssECI CCNNGG 7 cut(s) 498, 673, 705, 1389, 1542, 1647, 2564
BssNAI GTATAC 1 cut(s) 1446
BssNI GRCGYC 1 cut(s) 2498
BssT1I CCWWGG 6 cut(s) 498, 673, 705, 1389, 1542, 1647
Bst1107I GTATAC 1 cut(s) 1446
Bst2UI CCWGG 2 cut(s) 11, 2566
Bst4CI ACNGT 8 cut(s) 653, 681, 1930, 1971, 3111, 3206, 3810, 3819
Bst6I CTCTTC 5 cut(s) 82, 1371, 2037, 2136, 3155
BstACI GRCGYC 1 cut(s) 2498
BstAPI GCANNNNNTGC 1 cut(s) 339
BstAUI TGTACA 1 cut(s) 2790
BstBAI YACGTR 1 cut(s) 944
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 3 cut(s) 428, 1359, 2353
BstDSI CCRYGG 4 cut(s) 498, 1389, 1542, 1647
BstEII GGTNACC 1 cut(s) 3206
BstF5I GGATG 9 cut(s) 834, 1374, 1535, 1552, 1553, 1924, 2041, 2478, 2560
BstFNI CGCG 6 cut(s) 120, 2111, 2150, 2357, 2399, 3374
BstH2I RGCGCY 2 cut(s) 1744, 2501
BstHHI GCGC 5 cut(s) 1743, 2357, 2500, 3277, 3374
BstMAI GTCTC 6 cut(s) 1403, 1578, 1782, 1919, 2078, 3491
BstMCI CGRYCG 1 cut(s) 2154
BstMWI GCNNNNNNNGC 7 cut(s) 339, 983, 1666, 1877, 2101, 2315, 2451
BstNI CCWGG 2 cut(s) 11, 2566
BstNSI RCATGY 3 cut(s) 426, 430, 3573
BstPAI GACNNNNGTC 2 cut(s) 18, 1556
BstPI GGTNACC 1 cut(s) 3206
BstSCI CCNGG 4 cut(s) 9, 198, 2564, 3667
BstSLI GKGCMC 1 cut(s) 2412
BstUI CGCG 6 cut(s) 120, 2111, 2150, 2357, 2399, 3374
BstV1I GCAGC 8 cut(s) 345, 356, 986, 1813, 1892, 2757, 2993, 3837
BstV2I GAAGAC 2 cut(s) 94, 2502
BstX2I RGATCY 2 cut(s) 453, 494
BstXI CCANNNNNNTGG 3 cut(s) 1369, 1396, 1654
BstYI RGATCY 2 cut(s) 453, 494
BstZ17I GTATAC 1 cut(s) 1446
Bsu15I ATCGAT 1 cut(s) 1895
Bsu36I CCTNAGG 1 cut(s) 2285
BsuI GTATCC 2 cut(s) 933, 1198
BsuRI GGCC 9 cut(s) 1174, 1646, 2183, 2615, 3086, 3307, 3744, 4014, 4098
BsuTUI ATCGAT 1 cut(s) 1895
BtgI CCRYGG 4 cut(s) 498, 1389, 1542, 1647
BtsCI GGATG 9 cut(s) 834, 1374, 1535, 1552, 1553, 1924, 2041, 2478, 2560
BtsIMutI CAGTG 1 cut(s) 1218
BtuMI TCGCGA 1 cut(s) 2150
Cac8I GCNNGC 3 cut(s) 428, 1359, 2353
CciI TCATGA 2 cut(s) 1683, 3675
CfoI GCGC 5 cut(s) 1743, 2357, 2500, 3277, 3374
Cfr13I GGNCC 3 cut(s) 3084, 3560, 4012
ClaI ATCGAT 1 cut(s) 1895
CseI GACGC 2 cut(s) 479, 2405
Csp6I GTAC 7 cut(s) 773, 2791, 2910, 3061, 3396, 3573, 4033
CviQI GTAC 7 cut(s) 773, 2791, 2910, 3061, 3396, 3573, 4033
DinI GGCGCC 1 cut(s) 2499
DraI TTTAAA 2 cut(s) 2803, 3778
DrdI GACNNNNNNGTC 2 cut(s) 955, 2927
DseDI GACNNNNNNGTC 2 cut(s) 955, 2927
EaeI YGGCCR 3 cut(s) 1172, 3305, 3742
Eam1104I CTCTTC 5 cut(s) 82, 1371, 2037, 2136, 3155
EarI CTCTTC 5 cut(s) 82, 1371, 2037, 2136, 3155
EciI GGCGGA 1 cut(s) 1388
Ecl136II GAGCTC 2 cut(s) 447, 1236
Eco130I CCWWGG 6 cut(s) 498, 673, 705, 1389, 1542, 1647
Eco24I GRGCYC 2 cut(s) 449, 1238
Eco32I GATATC 1 cut(s) 1951
Eco47I GGWCC 1 cut(s) 3560
Eco53kI GAGCTC 2 cut(s) 447, 1236
Eco81I CCTNAGG 1 cut(s) 2285
Eco88I CYCGRG 2 cut(s) 1901, 2374
Eco91I GGTNACC 1 cut(s) 3206
EcoICRI GAGCTC 2 cut(s) 447, 1236
EcoO65I GGTNACC 1 cut(s) 3206
EcoRI GAATTC 4 cut(s) 1614, 2279, 2695, 3000
EcoRII CCWGG 2 cut(s) 9, 2564
EcoRV GATATC 1 cut(s) 1951
EcoT14I CCWWGG 6 cut(s) 498, 673, 705, 1389, 1542, 1647
EcoT22I ATGCAT 2 cut(s) 344, 428
EcoT38I GRGCYC 2 cut(s) 449, 1238
EgeI GGCGCC 1 cut(s) 2499
EheI GGCGCC 1 cut(s) 2499
ErhI CCWWGG 6 cut(s) 498, 673, 705, 1389, 1542, 1647
Esp3I CGTCTC 3 cut(s) 1403, 1578, 2078
FalI AAGNNNNNCTT 4 cut(s) 681, 713, 3264, 3296
FaqI GGGAC 3 cut(s) 495, 1895, 1957
FauNDI CATATG 2 cut(s) 17, 1623
FbaI TGATCA 4 cut(s) 1345, 1459, 2709, 3649
FblI GTMKAC 3 cut(s) 1445, 2757, 3113
Fnu4HI GCNGC 9 cut(s) 334, 370, 975, 1827, 1881, 2746, 3007, 3742, 3826
FokI GGATG 9 cut(s) 841, 1381, 1522, 1540, 1559, 1931, 2048, 2485, 2547
FriOI GRGCYC 2 cut(s) 449, 1238
Fsp4HI GCNGC 9 cut(s) 334, 370, 975, 1827, 1881, 2746, 3007, 3742, 3826
FspBI CTAG 7 cut(s) 674, 1287, 2213, 2459, 2913, 3692, 3756
GlaI GCGC 5 cut(s) 1742, 2356, 2499, 3276, 3373
GluI GCNGC 9 cut(s) 334, 370, 975, 1827, 1881, 2746, 3007, 3742, 3826
GsaI CCCAGC 1 cut(s) 671
GsuI CTGGAG 7 cut(s) 81, 1525, 1691, 2084, 2597, 2785, 3073
HaeII RGCGCY 2 cut(s) 1744, 2501
HaeIII GGCC 9 cut(s) 1174, 1646, 2183, 2615, 3086, 3307, 3744, 4014, 4098
HapII CCGG 3 cut(s) 199, 1890, 3669
HgaI GACGC 2 cut(s) 479, 2405
HhaI GCGC 5 cut(s) 1743, 2357, 2500, 3277, 3374
Hin1I GRCGYC 1 cut(s) 2498
Hin6I GCGC 5 cut(s) 1741, 2355, 2498, 3275, 3372
HinP1I GCGC 5 cut(s) 1741, 2355, 2498, 3275, 3372
HincII GTYRAC 5 cut(s) 1044, 1721, 2838, 3324, 3597
HindII GTYRAC 5 cut(s) 1044, 1721, 2838, 3324, 3597
HpaI GTTAAC 1 cut(s) 1044
HpaII CCGG 3 cut(s) 199, 1890, 3669
Hpy99I CGWCG 2 cut(s) 3306, 3403
HpyCH4III ACNGT 8 cut(s) 653, 681, 1930, 1971, 3111, 3206, 3810, 3819
HpyCH4IV ACGT 5 cut(s) 885, 943, 950, 1075, 3398
HpyF10VI GCNNNNNNNGC 7 cut(s) 339, 983, 1666, 1877, 2101, 2315, 2451
HpySE526I ACGT 5 cut(s) 885, 943, 950, 1075, 3398
Hsp92I GRCGYC 1 cut(s) 2498
HspAI GCGC 5 cut(s) 1741, 2355, 2498, 3275, 3372
KasI GGCGCC 1 cut(s) 2497
Ksp22I TGATCA 4 cut(s) 1345, 1459, 2709, 3649
KspAI GTTAAC 1 cut(s) 1044
LmnI GCTCC 4 cut(s) 1233, 2065, 2390, 2630
Lsp1109I GCAGC 8 cut(s) 345, 356, 986, 1813, 1892, 2757, 2993, 3837
MaeI CTAG 7 cut(s) 674, 1287, 2213, 2459, 2913, 3692, 3756
MaeII ACGT 5 cut(s) 885, 943, 950, 1075, 3398
MfeI CAATTG 1 cut(s) 3056
MflI RGATCY 2 cut(s) 453, 494
MhlI GDGCHC 3 cut(s) 449, 1238, 2412
MlsI TGGCCA 1 cut(s) 1174
MluNI TGGCCA 1 cut(s) 1174
Mly113I GGCGCC 1 cut(s) 2498
MlyI GAGTC 5 cut(s) 1516, 2176, 2366, 2878, 3238
MmeI TCCRAC 4 cut(s) 673, 1585, 2638, 3751
Mox20I TGGCCA 1 cut(s) 1174
Mph1103I ATGCAT 2 cut(s) 344, 428
MroXI GAANNNNTTC 1 cut(s) 1962
MscI TGGCCA 1 cut(s) 1174
MslI CAYNNNNRTG 5 cut(s) 425, 1367, 1547, 1652, 2978
Msp20I TGGCCA 1 cut(s) 1174
MspI CCGG 3 cut(s) 199, 1890, 3669
MspR9I CCNGG 4 cut(s) 11, 200, 2566, 3669
MunI CAATTG 1 cut(s) 3056
Mva1269I GAATGC 2 cut(s) 1829, 3632
MvaI CCWGG 2 cut(s) 11, 2566
MvnI CGCG 6 cut(s) 120, 2111, 2150, 2357, 2399, 3374
MwoI GCNNNNNNNGC 7 cut(s) 339, 983, 1666, 1877, 2101, 2315, 2451
NarI GGCGCC 1 cut(s) 2498
NciI CCSGG 2 cut(s) 200, 3669
NcoI CCATGG 4 cut(s) 498, 1389, 1542, 1647
NdeI CATATG 2 cut(s) 17, 1623
NlaIV GGNNCC 2 cut(s) 2499, 3166
NmeAIII GCCGAG 1 cut(s) 1605
NruI TCGCGA 1 cut(s) 2150
NsiI ATGCAT 2 cut(s) 344, 428
NspI RCATGY 3 cut(s) 426, 430, 3573
NspV TTCGAA 1 cut(s) 229
PacI TTAATTAA 1 cut(s) 1062
PaeI GCATGC 1 cut(s) 430
PaeR7I CTCGAG 1 cut(s) 1901
PagI TCATGA 2 cut(s) 1683, 3675
PciI ACATGT 1 cut(s) 3569
PcsI WCGNNNNNNNCGW 1 cut(s) 3135
PctI GAATGC 2 cut(s) 1829, 3632
PdmI GAANNNNTTC 1 cut(s) 1962
PfoI TCCNGGA 1 cut(s) 9
PkrI GCNGC 9 cut(s) 335, 371, 976, 1828, 1882, 2747, 3008, 3743, 3827
Ple19I CGATCG 1 cut(s) 2154
PleI GAGTC 5 cut(s) 1515, 2175, 2366, 2877, 3237
PluTI GGCGCC 1 cut(s) 2501
PpsI GAGTC 5 cut(s) 1515, 2175, 2366, 2877, 3237
Ppu21I YACGTR 1 cut(s) 944
PscI ACATGT 1 cut(s) 3569
PshAI GACNNNNGTC 2 cut(s) 18, 1556
PshBI ATTAAT 3 cut(s) 1062, 2991, 3026
PsiI TTATAA 2 cut(s) 317, 2955
Psp124BI GAGCTC 2 cut(s) 449, 1238
Psp6I CCWGG 2 cut(s) 9, 2564
PspEI GGTNACC 1 cut(s) 3206
PspFI CCCAGC 1 cut(s) 667
PspGI CCWGG 2 cut(s) 9, 2564
PspN4I GGNNCC 2 cut(s) 2499, 3166
PspPI GGNCC 3 cut(s) 3084, 3560, 4012
PspXI VCTCGAGB 1 cut(s) 1901
PsrI GAACNNNNNNTAC 2 cut(s) 1914, 1946
PsuI RGATCY 2 cut(s) 453, 494
PvuI CGATCG 1 cut(s) 2154
RruI TCGCGA 1 cut(s) 2150
RsaI GTAC 7 cut(s) 774, 2792, 2911, 3062, 3397, 3574, 4034
RsaNI GTAC 7 cut(s) 773, 2791, 2910, 3061, 3396, 3573, 4033
RseI CAYNNNNRTG 5 cut(s) 425, 1367, 1547, 1652, 2978
SacI GAGCTC 2 cut(s) 449, 1238
SatI GCNGC 9 cut(s) 334, 370, 975, 1827, 1881, 2746, 3007, 3742, 3826
Sau96I GGNCC 3 cut(s) 3084, 3560, 4012
ScaI AGTACT 1 cut(s) 2911
SchI GAGTC 5 cut(s) 1516, 2176, 2366, 2878, 3238
ScrFI CCNGG 4 cut(s) 11, 200, 2566, 3669
SduI GDGCHC 3 cut(s) 449, 1238, 2412
SfoI GGCGCC 1 cut(s) 2499
Sfr274I CTCGAG 1 cut(s) 1901
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 1 cut(s) 3560
SlaI CTCGAG 1 cut(s) 1901
SmiMI CAYNNNNRTG 5 cut(s) 425, 1367, 1547, 1652, 2978
SmlI CTYRAG 4 cut(s) 966, 1628, 1736, 1901
SmoI CTYRAG 4 cut(s) 966, 1628, 1736, 1901
SpeI ACTAGT 1 cut(s) 1286
SphI GCATGC 1 cut(s) 430
SsiI CCGC 4 cut(s) 120, 1373, 1475, 3741
SspDI GGCGCC 1 cut(s) 2497
SspI AATATT 2 cut(s) 3523, 3876
SspMI CTAG 7 cut(s) 674, 1287, 2213, 2459, 2913, 3692, 3756
SstI GAGCTC 2 cut(s) 449, 1238
StyD4I CCNGG 4 cut(s) 9, 198, 2564, 3667
StyI CCWWGG 6 cut(s) 498, 673, 705, 1389, 1542, 1647
TaaI ACNGT 8 cut(s) 653, 681, 1930, 1971, 3111, 3206, 3810, 3819
TaiI ACGT 5 cut(s) 888, 946, 953, 1078, 3401
TatI WGTACW 5 cut(s) 2790, 2909, 3060, 3572, 4032
TauI GCSGC 1 cut(s) 3744
TscAI CASTG 1 cut(s) 1225
TseI GCWGC 8 cut(s) 333, 369, 974, 1826, 1880, 2745, 3006, 3825
TspGWI ACGGA 2 cut(s) 1546, 2358
TspRI CASTG 1 cut(s) 1225
VneI GTGCAC 1 cut(s) 2408
VpaK11BI GGWCC 1 cut(s) 3560
VspI ATTAAT 3 cut(s) 1062, 2991, 3026
XceI RCATGY 3 cut(s) 426, 430, 3573
XcmI CCANNNNNNNNNTGG 1 cut(s) 2623
XhoI CTCGAG 1 cut(s) 1901
XmaJI CCTAGG 1 cut(s) 673
XmiI GTMKAC 3 cut(s) 1445, 2757, 3113
XmnI GAANNNNTTC 1 cut(s) 1962
XspI CTAG 7 cut(s) 674, 1287, 2213, 2459, 2913, 3692, 3756
ZrmI AGTACT 1 cut(s) 2911
Zsp2I ATGCAT 2 cut(s) 344, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.