Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0002897

Physical Location & Seq

Genomic Coordinates Reverse (-)

21335787 .. 21339821

Gene Structure 4035 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2682 bp

ATGGAGGTTGAGAAGAGACGTTCTAAAGGGGGCTTTCTTAACTTGTTTGATTGGAATGGAAAATCTCGGAAGAAGCTCTTCTTGGCCAGTTCTGAATCATCGGGATTAAAGCAAGGAAAAGAAAATGTGGAGAGTTTCCAGAAGTCCGGGCTCTGTAGGGTAGAGGTGGATGAAAGTGGAACTGGAACCACTTCGAGTAATAAAGGAAGTGGTGATTGGAATTGTGCTTCATCAGTGACTAGTGATGAGGGATCTAAGGCTAGAGCCCCTGGTGTAGTTGCTAGATTAATGGGTCTGGATTCATTGCCTGCTTTAACTGCCCCCGACCCCGAGCCATCTTCTGCCCTACTCTTTGACTCCCAGTCGCTTAAAGCATCTCAATATGAGAGGAGCGGTCGTAATTTGTGGAGTGATTTGTATGCCACGGAGTACAGAAATGTGCCAAAGAAGCTGGATAGGTTTTCTTGGAATCTTGTAGAGCCTAGGACACAGGGGGTGCCGAGCCACCCAATTGAGAGATTTCAAACAGAAGCTTTGCCTCCAAAATCAGCCAAGTCAATTCCAGTTACTCACCATAAGCTTTTGTCGCCTATCAAGAGTCCTGGCTTCATTCCACCAAAAAATGCAGCTTATGTAATGGAGGCGGCCTCAAAGATCATTGAGGCAAGCCCTCGAGCATCTGCCAGGAGCAAAGTGTCATCTGTAGGGCCTTCTTCGATTCCCTTGAGAATTCGGGATTTGAAAGAGAAGATGGAAGCTGTGCATAAAGCATCAGGACCTGAAAGACCCAAGGAAGCTAGTGACTTCAGGTATATGAAAGGACTGCCTAGTGACAGGCACCATAACAGGTCAGATAATGTACCAGTAGCCAAGGCATCTGTGGATTCAGAAAAACAAAGTTACAGGGATGTAAGGAATAAGGGCAAATCAGCATCCCTTGCAGAACAAGCAAAGTTCAATATTCAGAAAAGGGAGGGGTCGACTTCTTGTCGTAATAGAAGTTTTATGAACCAGAAGGAACAGAATGAGGTCAAACAAAATCAGTTTTCCAAGAGCAAGTCAAGTACACAAACATCTACGCATAAGAGAACTTCCATTGAGAGCGCCAGAACTGCGCTCAAGCAGAATAACCAGAAGCAGAATTGTGTATCTAACAAAGATAAAATGACTTCAAAAAGTATGGTTCCAAATCAGCCTACTAGAAGAATGCGGCCACCAAATGGCTCTACTGGGCCAATGAAAACTGTGAATAAGACTATTGTAAGCTCTGAAATTGGATCCGGAAAGTTGGGCTCAAGGGCGACTGCTAATGGAAAAGAGTTTCCATTATCCACAAGAAAGATTGTTTCTGGAAAGGTAAGGTCTGTTCATCAGGATGATCATTCAGAAGAAACTGTTTCCGGTGATTCATATATCGGTGGAGATGAAAAGTCAGTGAAATGTAATGTTTCAAGGAACGGGTGTTCCAATTTGGGCGCAGATAATAGGAACCAAGGCATGGATGTTATTTCATTTACATTCACGTCTCCCCTGAAAAGATCATTCTCTGAATTGCAGTCACCTGGTCAACTCACAAGTATAAATAACAGCTTTTATATTGATTCTTCTGCTCACAACGATCAGCAGTTCCATCCAGAGAATTTCACATTATCTTCTCCTGGGTTGAATGTGATTGGTGGTGATGCTTTGAGTGTCCTTCTGGAACAAAAGCTTCAAGAACTGTCATGTAAAGTTGAGTCATCTCAGCGTAACCAATCCAGTGAAGTGACTTCTGGTACTTCTATGTCTTGCTTGCAAGATATGGTTTCCAGTGTGGGAAGCACTACATCAGGAGGCAAGGGGTTCAAAGTTGGTTTAACCAAAGATAAAACTGACAGCACAGACGACTATGATTGCTTGTCAGTTGAGGTTAACCAGCAGTGGCAGGGATCAGAAGGGGTGGAAGAGTGTAGCAGTAGCAGTAGAAACATTACAACTGGAAATGAATTTGATGGACGTCGACACTCATTTGAGAGTCGAACCTGCACAGATAACAGAAGTAGCGCCAAAGGCAGTGACAGTGAGAGATGTTCATTGGCTCAACTTCAAGATCAAGGCAGTTCACTCTCTTCATATGGCGTTTCAGAGTTCCTCTACTCAGCCTTTTCAGCTTCTACAGGGGATGCGAGCGGAAAGAGTACGACTATGATATCAGGTTCGCATTCAAATGATTGGGAACTGGAATATGTGAGATACGTCCTTAGCAATGTAGATCTAGAGATGGAAGATTTCGCCTTGGGTGATGCTCAACACGTCATCACCCCAAGTCTCTTTGAGCATTTGGAGAATCAAGAACAAGAAGAGTATCCGAAGCTCGAGCAGAAGATTGTGTTTGACTGCGTAAATGAAGCCCTGCAGTTCAGATGCAAGCAAATATTTGTGGGAAGCCGCAAAGCGTGGGATAGATTGACCGCATTGTCTCAGACGAAGGGACGATTAGCTGAGGAATTGCACAAAGAGATTTCGGGTTGGAAAAACATGAGAGAGCTGATGGGGGATGAGCTTGTGGACAAGGACATGAGCACGCAGCGTGGAAGATGGCTCGACTTTGAAGTTGAAGCGTTTGAGGAGGGTCTAGAGATTGAGAAGGGGATACTAAATTGCCTGGTGGAAGAGTTGGTGTCTGATTTTTTGAACTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4378 VARLMGL

Protein Analysis

894

Amino Acids

98.48

Weight (kDa)

7.8

Isoelectric Point (pI)

54.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VARLMGL	PF14383	77 - 104	1.1e-13	DUF761-associated sequence motif
DUF4378	PF14309	739 - 885	4.1e-33	Domain of unknown function (DUF4378)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002897)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05740 AT3G05740
fragaria_vesca	FvH4_6g07200 FvH4_6g07210 FvH4_6g07210 FvH4_6g07210 FvH4_6g07210
malus_domestica	MD04G1195500.v1.1 MD12G1207600.v1.1 MD12G1207700.v1.1 MD12G1207800.v1.1
prunus_persica	Prupe.6G315200_v2.0.a1 Prupe.6G315300_v2.0.a1 Prupe.6G315300_v2.0.a1 Prupe.6G315300_v2.0.a1
pyrus_communis	pycom04g17270 pycom12g19520 pycom12g19530
rosa_chinensis	RchiOBHm_Chr3g0456831 RchiOBHm_Chr3g0456841
rosa_laevigata	RLG00000025275
rosa_multiflora	Rmu_co8434835.1_g000001 Rmu_sc0000133.1_g000020
rosa_roxburghii	Rroxscaffold_6G00422450 Rroxscaffold_6G00422460
rosa_rugosa	Rorug03G0016100 Rorug03G0016200 Rorug03G0016300.1
rosa_samantha	Rh3AG076400 Rh3AG076600 Rh3BG078200 Rh3BG078400 Rh3CG078000 Rh3CG078200 Rh3DG080100 Rh3DG080200
rosa_wichuraiana	Rw3G006560 Rw3G006570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2455
AatII	GACGTC	1 cut(s)	1999
Acc36I	ACCTGC	1 cut(s)	2030
AccB1I	GGYRCC	2 cut(s)	496, 837
AccB7I	CCANNNNNTGG	2 cut(s)	1220, 1498
AccBSI	CCGCTC	2 cut(s)	393, 2169
AccI	GTMKAC	2 cut(s)	980, 1999
AccIII	TCCGGA	1 cut(s)	1280
AciI	CCGC	6 cut(s)	393, 644, 1210, 2169, 2428, 2451
AclWI	GGATC	4 cut(s)	259, 1272, 1285, 1936
AcoI	YGGCCR	2 cut(s)	84, 1211
AcsI	RAATTY	3 cut(s)	729, 1639, 1985
AcuI	CTGAAG	1 cut(s)	790
AcyI	GRCGYC	1 cut(s)	1996
AfaI	GTAC	5 cut(s)	431, 861, 1066, 1777, 2179
AfiI	CCNNNNNNNGG	2 cut(s)	1220, 1498
AflIII	ACRYGT	1 cut(s)	2290
AhdI	GACNNNNNGTC	2 cut(s)	361, 987
AhlI	ACTAGT	1 cut(s)	239
AjiI	CACGTC	2 cut(s)	1524, 2293
AjnI	CCWGG	6 cut(s)	268, 601, 683, 1561, 1657, 2643
AjuI	GAANNNNNNNTTGG	4 cut(s)	65, 97, 199, 231
AloI	GAACNNNNNNTCC	4 cut(s)	1447, 1448, 1479, 1480
Alw21I	GWGCWC	1 cut(s)	2564
Alw26I	GTCTC	4 cut(s)	10, 1530, 2312, 2463
AlwI	GGATC	4 cut(s)	259, 1272, 1285, 1936
AlwNI	CAGNNNCTG	1 cut(s)	779
Ama87I	CYCGRG	3 cut(s)	329, 672, 2354
Aor13HI	TCCGGA	1 cut(s)	1280
AoxI	GGCC	5 cut(s)	84, 645, 707, 1211, 1232
ApeKI	GCWGC	2 cut(s)	626, 2566
ApoI	RAATTY	3 cut(s)	729, 1639, 1985
AseI	ATTAAT	1 cut(s)	287
Asp700I	GAANNNNTTC	2 cut(s)	77, 190
AspA2I	CCTAGG	1 cut(s)	482
AspLEI	GCGC	4 cut(s)	1106, 1117, 1478, 2045
AspS9I	GGNCC	3 cut(s)	707, 776, 1232
AsuC2I	CCSGG	1 cut(s)	148
AsuHPI	GGTGA	7 cut(s)	224, 563, 1415, 1551, 1691, 2290, 2291
AvaI	CYCGRG	3 cut(s)	329, 672, 2354
AvaII	GGWCC	1 cut(s)	776
AvrII	CCTAGG	1 cut(s)	482
BalI	TGGCCA	1 cut(s)	86
BamHI	GGATCC	1 cut(s)	1277
BanI	GGYRCC	2 cut(s)	496, 837
BanII	GRGCYC	3 cut(s)	153, 268, 1295
Bbv12I	GWGCWC	1 cut(s)	2564
BbvCI	CCTCAGC	1 cut(s)	2481
BbvI	GCAGC	2 cut(s)	638, 2578
BccI	CCATC	7 cut(s)	343, 745, 1638, 1985, 2254, 2524, 2571
BciT130I	CCWGG	6 cut(s)	270, 603, 685, 1563, 1659, 2645
BciVI	GTATCC	2 cut(s)	2355, 2625
BclI	TGATCA	1 cut(s)	1378
BcnI	CCSGG	1 cut(s)	148
BcoDI	GTCTC	4 cut(s)	10, 1530, 2312, 2463
BcuI	ACTAGT	1 cut(s)	239
BfaI	CTAG	9 cut(s)	240, 261, 282, 483, 798, 828, 1200, 2255, 2615
BfmI	CTRYAG	4 cut(s)	154, 702, 2154, 2393
BfoI	RGCGCY	2 cut(s)	1107, 2046
BfuAI	ACCTGC	1 cut(s)	2030
BfuI	GTATCC	2 cut(s)	2355, 2625
BglII	AGATCT	1 cut(s)	2251
BisI	GCNGC	5 cut(s)	627, 645, 1211, 2428, 2567
BlnI	CCTAGG	1 cut(s)	482
BlsI	GCNGC	5 cut(s)	628, 646, 1212, 2429, 2568
Bme1390I	CCNGG	7 cut(s)	148, 270, 603, 685, 1563, 1659, 2645
Bme18I	GGWCC	1 cut(s)	776
BmeRI	GACNNNNNGTC	2 cut(s)	361, 987
BmeT110I	CYCGRG	3 cut(s)	329, 672, 2354
BmgBI	CACGTC	2 cut(s)	1524, 2293
BmgT120I	GGNCC	3 cut(s)	707, 776, 1232
BmiI	GGNNCC	6 cut(s)	187, 498, 839, 1185, 1279, 1490
BmrFI	CCNGG	7 cut(s)	148, 270, 603, 685, 1563, 1659, 2645
BmrI	ACTGGG	2 cut(s)	355, 1239
BmsI	GCATC	9 cut(s)	383, 686, 779, 884, 941, 1672, 2152, 2272, 2393
BmuI	ACTGGG	2 cut(s)	355, 1239
BplI	GAGNNNNNCTC	2 cut(s)	632, 664
Bpu10I	CCTNAGC	2 cut(s)	2240, 2481
BpuEI	CTTGAG	3 cut(s)	745, 1103, 1279
BpuMI	CCSGG	1 cut(s)	148
BsaHI	GRCGYC	1 cut(s)	1996
BsaJI	CCNNGG	8 cut(s)	268, 423, 482, 789, 870, 1492, 1658, 2274
BsaWI	WCCGGW	2 cut(s)	1280, 1400
BsaXI	ACNNNNNCTCC	2 cut(s)	382, 412
Bsc4I	CCNNNNNNNGG	2 cut(s)	1220, 1498
Bse3DI	GCAATG	2 cut(s)	302, 2251
BseAI	TCCGGA	1 cut(s)	1280
BseBI	CCWGG	6 cut(s)	270, 603, 685, 1563, 1659, 2645
BseDI	CCNNGG	8 cut(s)	268, 423, 482, 789, 870, 1492, 1658, 2274
BseGI	GGATG	8 cut(s)	175, 913, 932, 1381, 1507, 1630, 2167, 2542
BseLI	CCNNNNNNNGG	2 cut(s)	1220, 1498
BseMI	GCAATG	2 cut(s)	302, 2251
BseMII	CTCAG	4 cut(s)	1757, 2151, 2472, 2474
BseRI	GAGGAG	2 cut(s)	403, 2621
BseXI	GCAGC	2 cut(s)	638, 2578
BsgI	GTGCAG	1 cut(s)	2008
Bsh1285I	CGRYCG	1 cut(s)	397
BshFI	GGCC	5 cut(s)	86, 647, 709, 1213, 1234
BshNI	GGYRCC	2 cut(s)	496, 837
BsiEI	CGRYCG	1 cut(s)	397
BsiHKAI	GWGCWC	1 cut(s)	2564
BsiHKCI	CYCGRG	3 cut(s)	329, 672, 2354
BsiSI	CCGG	3 cut(s)	147, 1281, 1401
BslFI	GGGAC	1 cut(s)	2484
BslI	CCNNNNNNNGG	2 cut(s)	1220, 1498
BsmAI	GTCTC	4 cut(s)	10, 1530, 2312, 2463
BsmBI	CGTCTC	2 cut(s)	10, 1530
BsmFI	GGGAC	1 cut(s)	2484
BsmI	GAATGC	2 cut(s)	1212, 2200
BsnI	GGCC	5 cut(s)	86, 647, 709, 1213, 1234
BsoBI	CYCGRG	3 cut(s)	329, 672, 2354
Bsp1286I	GDGCHC	4 cut(s)	153, 268, 1295, 2564
Bsp13I	TCCGGA	1 cut(s)	1280
Bsp143I	GATC	9 cut(s)	251, 654, 1277, 1378, 1538, 1618, 1928, 2089, 2251
BspACI	CCGC	6 cut(s)	393, 644, 1210, 2169, 2428, 2451
BspANI	GGCC	5 cut(s)	86, 647, 709, 1213, 1234
BspCNI	CTCAG	4 cut(s)	1756, 2150, 2473, 2473
BspEI	TCCGGA	1 cut(s)	1280
BspLI	GGNNCC	6 cut(s)	187, 498, 839, 1185, 1279, 1490
BspMAI	CTGCAG	1 cut(s)	2397
BspMI	ACCTGC	1 cut(s)	2030
BspPI	GGATC	4 cut(s)	259, 1272, 1285, 1936
BspQI	GCTCTTC	1 cut(s)	83
BspT107I	GGYRCC	2 cut(s)	496, 837
BsrBI	CCGCTC	2 cut(s)	393, 2169
BsrDI	GCAATG	2 cut(s)	302, 2251
BssECI	CCNNGG	8 cut(s)	268, 423, 482, 789, 870, 1492, 1658, 2274
BssMI	GATC	9 cut(s)	251, 654, 1277, 1378, 1538, 1618, 1928, 2089, 2251
BssNI	GRCGYC	1 cut(s)	1996
BssT1I	CCWWGG	5 cut(s)	482, 789, 870, 1492, 2274
Bst2UI	CCWGG	6 cut(s)	270, 603, 685, 1563, 1659, 2645
Bst4CI	ACNGT	4 cut(s)	1246, 1396, 1722, 2060
Bst6I	CTCTTC	6 cut(s)	8, 83, 1938, 2113, 2334, 2646
BstACI	GRCGYC	1 cut(s)	1996
BstAPI	GCANNNNNTGC	1 cut(s)	938
BstC8I	GCNNGC	6 cut(s)	309, 667, 1793, 2167, 2408, 2564
BstDEI	CTNAG	6 cut(s)	255, 1743, 2137, 2240, 2460, 2481
BstDSI	CCRYGG	1 cut(s)	423
BstF5I	GGATG	8 cut(s)	175, 913, 932, 1381, 1507, 1630, 2167, 2542
BstH2I	RGCGCY	2 cut(s)	1107, 2046
BstHHI	GCGC	4 cut(s)	1106, 1117, 1478, 2045
BstKTI	GATC	9 cut(s)	254, 657, 1280, 1381, 1541, 1621, 1931, 2092, 2254
BstMAI	GTCTC	4 cut(s)	10, 1530, 2312, 2463
BstMBI	GATC	9 cut(s)	251, 654, 1277, 1378, 1538, 1618, 1928, 2089, 2251
BstMCI	CGRYCG	1 cut(s)	397
BstMWI	GCNNNNNNNGC	8 cut(s)	317, 448, 586, 938, 947, 1112, 2049, 2147
BstNI	CCWGG	6 cut(s)	270, 603, 685, 1563, 1659, 2645
BstSCI	CCNGG	7 cut(s)	146, 268, 601, 683, 1561, 1657, 2643
BstSFI	CTRYAG	4 cut(s)	154, 702, 2154, 2393
BstV1I	GCAGC	2 cut(s)	638, 2578
BstX2I	RGATCY	3 cut(s)	251, 1277, 2251
BstYI	RGATCY	3 cut(s)	251, 1277, 2251
BsuI	GTATCC	2 cut(s)	2355, 2625
BsuRI	GGCC	5 cut(s)	86, 647, 709, 1213, 1234
BtgI	CCRYGG	1 cut(s)	423
BtrI	CACGTC	2 cut(s)	1524, 2293
BtsCI	GGATG	8 cut(s)	175, 913, 932, 1381, 1507, 1630, 2167, 2542
BtsI	GCAGTG	2 cut(s)	1925, 2059
BtsIMutI	CAGTG	7 cut(s)	240, 1440, 1765, 1816, 1925, 2059, 2065
BveI	ACCTGC	1 cut(s)	2030
Cac8I	GCNNGC	6 cut(s)	309, 667, 1793, 2167, 2408, 2564
CaiI	CAGNNNCTG	1 cut(s)	779
CfoI	GCGC	4 cut(s)	1106, 1117, 1478, 2045
Cfr13I	GGNCC	3 cut(s)	707, 776, 1232
CsiI	ACCWGGT	1 cut(s)	1561
Csp6I	GTAC	5 cut(s)	430, 860, 1065, 1776, 2178
CviAII	CATG	4 cut(s)	1498, 1725, 2518, 2557
CviQI	GTAC	5 cut(s)	430, 860, 1065, 1776, 2178
DdeI	CTNAG	6 cut(s)	255, 1743, 2137, 2240, 2460, 2481
DpnI	GATC	9 cut(s)	253, 656, 1279, 1380, 1540, 1620, 1930, 2091, 2253
DpnII	GATC	9 cut(s)	251, 654, 1277, 1378, 1538, 1618, 1928, 2089, 2251
DrdI	GACNNNNNNGTC	1 cut(s)	2455
DriI	GACNNNNNGTC	2 cut(s)	361, 987
DseDI	GACNNNNNNGTC	1 cut(s)	2455
EaeI	YGGCCR	2 cut(s)	84, 1211
Eam1104I	CTCTTC	6 cut(s)	8, 83, 1938, 2113, 2334, 2646
Eam1105I	GACNNNNNGTC	2 cut(s)	361, 987
EarI	CTCTTC	6 cut(s)	8, 83, 1938, 2113, 2334, 2646
Eco130I	CCWWGG	5 cut(s)	482, 789, 870, 1492, 2274
Eco24I	GRGCYC	3 cut(s)	153, 268, 1295
Eco32I	GATATC	1 cut(s)	2190
Eco47I	GGWCC	1 cut(s)	776
Eco57I	CTGAAG	1 cut(s)	790
Eco88I	CYCGRG	3 cut(s)	329, 672, 2354
EcoO109I	RGGNCCY	2 cut(s)	707, 776
EcoRI	GAATTC	1 cut(s)	729
EcoRII	CCWGG	6 cut(s)	268, 601, 683, 1561, 1657, 2643
EcoRV	GATATC	1 cut(s)	2190
EcoT14I	CCWWGG	5 cut(s)	482, 789, 870, 1492, 2274
EcoT38I	GRGCYC	3 cut(s)	153, 268, 1295
ErhI	CCWWGG	5 cut(s)	482, 789, 870, 1492, 2274
Esp3I	CGTCTC	2 cut(s)	10, 1530
FaeI	CATG	4 cut(s)	1501, 1728, 2521, 2560
FalI	AAGNNNNNCTT	4 cut(s)	62, 94, 65, 97
FaqI	GGGAC	1 cut(s)	2484
FatI	CATG	4 cut(s)	1497, 1724, 2517, 2556
FauNDI	CATATG	1 cut(s)	2113
FbaI	TGATCA	1 cut(s)	1378
FblI	GTMKAC	2 cut(s)	980, 1999
Fnu4HI	GCNGC	5 cut(s)	627, 645, 1211, 2428, 2567
FokI	GGATG	8 cut(s)	182, 919, 920, 1388, 1514, 1617, 2174, 2549
FriOI	GRGCYC	3 cut(s)	153, 268, 1295
Fsp4HI	GCNGC	5 cut(s)	627, 645, 1211, 2428, 2567
FspBI	CTAG	9 cut(s)	240, 261, 282, 483, 798, 828, 1200, 2255, 2615
GlaI	GCGC	4 cut(s)	1105, 1116, 1477, 2044
GluI	GCNGC	5 cut(s)	627, 645, 1211, 2428, 2567
HaeII	RGCGCY	2 cut(s)	1107, 2046
HaeIII	GGCC	5 cut(s)	86, 647, 709, 1213, 1234
HapII	CCGG	3 cut(s)	147, 1281, 1401
HhaI	GCGC	4 cut(s)	1106, 1117, 1478, 2045
Hin1I	GRCGYC	1 cut(s)	1996
Hin1II	CATG	4 cut(s)	1501, 1728, 2521, 2560
Hin6I	GCGC	4 cut(s)	1104, 1115, 1476, 2043
HinP1I	GCGC	4 cut(s)	1104, 1115, 1476, 2043
HincII	GTYRAC	4 cut(s)	981, 1568, 1912, 2000
HindII	GTYRAC	4 cut(s)	981, 1568, 1912, 2000
HindIII	AAGCTT	3 cut(s)	531, 578, 1709
HpaI	GTTAAC	1 cut(s)	1912
HpaII	CCGG	3 cut(s)	147, 1281, 1401
HphI	GGTGA	7 cut(s)	224, 563, 1415, 1551, 1691, 2290, 2291
Hpy166II	GTNNAC	7 cut(s)	981, 1067, 1568, 1912, 2000, 2102, 2548
Hpy8I	GTNNAC	7 cut(s)	981, 1067, 1568, 1912, 2000, 2102, 2548
Hpy99I	CGWCG	1 cut(s)	2001
HpyAV	CCTTC	6 cut(s)	720, 1009, 1706, 1928, 2461, 2620
HpyCH4III	ACNGT	4 cut(s)	1246, 1396, 1722, 2060
HpyCH4IV	ACGT	5 cut(s)	19, 1523, 1996, 2235, 2292
HpyCH4V	TGCA	9 cut(s)	626, 763, 941, 1555, 1795, 2025, 2395, 2406, 2491
HpyF10VI	GCNNNNNNNGC	8 cut(s)	317, 448, 586, 938, 947, 1112, 2049, 2147
HpyF3I	CTNAG	6 cut(s)	255, 1743, 2137, 2240, 2460, 2481
HpySE526I	ACGT	5 cut(s)	19, 1523, 1996, 2235, 2292
Hsp92I	GRCGYC	1 cut(s)	1996
Hsp92II	CATG	4 cut(s)	1501, 1728, 2521, 2560
HspAI	GCGC	4 cut(s)	1104, 1115, 1476, 2043
Kpn2I	TCCGGA	1 cut(s)	1280
Ksp22I	TGATCA	1 cut(s)	1378
KspAI	GTTAAC	1 cut(s)	1912
Kzo9I	GATC	9 cut(s)	251, 654, 1277, 1378, 1538, 1618, 1928, 2089, 2251
LguI	GCTCTTC	1 cut(s)	83
LmnI	GCTCC	2 cut(s)	390, 687
Lsp1109I	GCAGC	2 cut(s)	638, 2578
LweI	GCATC	9 cut(s)	383, 686, 779, 884, 941, 1672, 2152, 2272, 2393
MabI	ACCWGGT	1 cut(s)	1561
MaeI	CTAG	9 cut(s)	240, 261, 282, 483, 798, 828, 1200, 2255, 2615
MaeII	ACGT	5 cut(s)	19, 1523, 1996, 2235, 2292
MaeIII	GTNAC	9 cut(s)	235, 565, 800, 830, 899, 1557, 1748, 1765, 2054
MalI	GATC	9 cut(s)	253, 656, 1279, 1380, 1540, 1620, 1930, 2091, 2253
MbiI	CCGCTC	2 cut(s)	393, 2169
MboI	GATC	9 cut(s)	251, 654, 1277, 1378, 1538, 1618, 1928, 2089, 2251
MfeI	CAATTG	1 cut(s)	510
MflI	RGATCY	3 cut(s)	251, 1277, 2251
MhlI	GDGCHC	4 cut(s)	153, 268, 1295, 2564
MlsI	TGGCCA	1 cut(s)	86
MluNI	TGGCCA	1 cut(s)	86
MlyI	GAGTC	4 cut(s)	350, 607, 1745, 2023
MmeI	TCCRAC	1 cut(s)	2489
Mox20I	TGGCCA	1 cut(s)	86
MroI	TCCGGA	1 cut(s)	1280
MroXI	GAANNNNTTC	2 cut(s)	77, 190
MscI	TGGCCA	1 cut(s)	86
MseI	TTAA	7 cut(s)	39, 107, 287, 314, 369, 1856, 1911
MslI	CAYNNNNRTG	2 cut(s)	1374, 2205
Msp20I	TGGCCA	1 cut(s)	86
MspI	CCGG	3 cut(s)	147, 1281, 1401
MspR9I	CCNGG	7 cut(s)	148, 270, 603, 685, 1563, 1659, 2645
MunI	CAATTG	1 cut(s)	510
Mva1269I	GAATGC	2 cut(s)	1212, 2200
MvaI	CCWGG	6 cut(s)	270, 603, 685, 1563, 1659, 2645
MwoI	GCNNNNNNNGC	8 cut(s)	317, 448, 586, 938, 947, 1112, 2049, 2147
NciI	CCSGG	1 cut(s)	148
NdeI	CATATG	1 cut(s)	2113
NdeII	GATC	9 cut(s)	251, 654, 1277, 1378, 1538, 1618, 1928, 2089, 2251
NlaIII	CATG	4 cut(s)	1501, 1728, 2521, 2560
NlaIV	GGNNCC	6 cut(s)	187, 498, 839, 1185, 1279, 1490
NmeAIII	GCCGAG	1 cut(s)	525
NmuCI	GTSAC	6 cut(s)	235, 800, 830, 1557, 1765, 2054
PaeR7I	CTCGAG	2 cut(s)	672, 2354
PciSI	GCTCTTC	1 cut(s)	83
PctI	GAATGC	2 cut(s)	1212, 2200
PdmI	GAANNNNTTC	2 cut(s)	77, 190
PfeI	GAWTC	8 cut(s)	95, 299, 469, 718, 884, 1406, 1601, 2326
PflMI	CCANNNNNTGG	2 cut(s)	1220, 1498
PkrI	GCNGC	5 cut(s)	628, 646, 1212, 2429, 2568
PleI	GAGTC	4 cut(s)	350, 606, 1744, 2022
PpsI	GAGTC	4 cut(s)	350, 606, 1744, 2022
PpuMI	RGGWCCY	1 cut(s)	776
PshBI	ATTAAT	1 cut(s)	287
Psp5II	RGGWCCY	1 cut(s)	776
Psp6I	CCWGG	6 cut(s)	268, 601, 683, 1561, 1657, 2643
PspGI	CCWGG	6 cut(s)	268, 601, 683, 1561, 1657, 2643
PspN4I	GGNNCC	6 cut(s)	187, 498, 839, 1185, 1279, 1490
PspPI	GGNCC	3 cut(s)	707, 776, 1232
PspPPI	RGGWCCY	1 cut(s)	776
PspXI	VCTCGAGB	2 cut(s)	672, 2354
PsrI	GAACNNNNNNTAC	2 cut(s)	1350, 1382
PstI	CTGCAG	1 cut(s)	2397
PstNI	CAGNNNCTG	1 cut(s)	779
PsuI	RGATCY	3 cut(s)	251, 1277, 2251
RsaI	GTAC	5 cut(s)	431, 861, 1066, 1777, 2179
RsaNI	GTAC	5 cut(s)	430, 860, 1065, 1776, 2178
RseI	CAYNNNNRTG	2 cut(s)	1374, 2205
SalI	GTCGAC	2 cut(s)	979, 1998
SapI	GCTCTTC	1 cut(s)	83
SaqAI	TTAA	7 cut(s)	39, 107, 287, 314, 369, 1856, 1911
SatI	GCNGC	5 cut(s)	627, 645, 1211, 2428, 2567
Sau3AI	GATC	9 cut(s)	251, 654, 1277, 1378, 1538, 1618, 1928, 2089, 2251
Sau96I	GGNCC	3 cut(s)	707, 776, 1232
SchI	GAGTC	4 cut(s)	350, 607, 1745, 2023
ScrFI	CCNGG	7 cut(s)	148, 270, 603, 685, 1563, 1659, 2645
SduI	GDGCHC	4 cut(s)	153, 268, 1295, 2564
SexAI	ACCWGGT	1 cut(s)	1561
SfaNI	GCATC	9 cut(s)	383, 686, 779, 884, 941, 1672, 2152, 2272, 2393
SfcI	CTRYAG	4 cut(s)	154, 702, 2154, 2393
Sfr274I	CTCGAG	2 cut(s)	672, 2354
SinI	GGWCC	1 cut(s)	776
SlaI	CTCGAG	2 cut(s)	672, 2354
SmiMI	CAYNNNNRTG	2 cut(s)	1374, 2205
SmlI	CTYRAG	5 cut(s)	672, 724, 1118, 1294, 2354
SmoI	CTYRAG	5 cut(s)	672, 724, 1118, 1294, 2354
SpeI	ACTAGT	1 cut(s)	239
SsiI	CCGC	6 cut(s)	393, 644, 1210, 2169, 2428, 2451
SspI	AATATT	2 cut(s)	961, 2415
SspMI	CTAG	9 cut(s)	240, 261, 282, 483, 798, 828, 1200, 2255, 2615
StyD4I	CCNGG	7 cut(s)	146, 268, 601, 683, 1561, 1657, 2643
StyI	CCWWGG	5 cut(s)	482, 789, 870, 1492, 2274
TaaI	ACNGT	4 cut(s)	1246, 1396, 1722, 2060
TaiI	ACGT	5 cut(s)	22, 1526, 1999, 2238, 2295
TaqI	TCGA	8 cut(s)	194, 673, 716, 980, 1999, 2017, 2355, 2583
TatI	WGTACW	2 cut(s)	429, 1064
TauI	GCSGC	3 cut(s)	647, 1213, 2430
TfiI	GAWTC	8 cut(s)	95, 299, 469, 718, 884, 1406, 1601, 2326
Tru1I	TTAA	7 cut(s)	39, 107, 287, 314, 369, 1856, 1911
Tru9I	TTAA	7 cut(s)	39, 107, 287, 314, 369, 1856, 1911
TscAI	CASTG	7 cut(s)	240, 1440, 1765, 1816, 1925, 2059, 2065
TseFI	GTSAC	6 cut(s)	235, 800, 830, 1557, 1765, 2054
TseI	GCWGC	2 cut(s)	626, 2566
Tsp45I	GTSAC	6 cut(s)	235, 800, 830, 1557, 1765, 2054
TspGWI	ACGGA	1 cut(s)	440
TspRI	CASTG	7 cut(s)	240, 1440, 1765, 1816, 1925, 2059, 2065
Van91I	CCANNNNNTGG	2 cut(s)	1220, 1498
VpaK11BI	GGWCC	1 cut(s)	776
VspI	ATTAAT	1 cut(s)	287
XapI	RAATTY	3 cut(s)	729, 1639, 1985
XbaI	TCTAGA	2 cut(s)	2254, 2614
XcmI	CCANNNNNNNNNTGG	1 cut(s)	877
XhoI	CTCGAG	2 cut(s)	672, 2354
XmaJI	CCTAGG	1 cut(s)	482
XmiI	GTMKAC	2 cut(s)	980, 1999
XmnI	GAANNNNTTC	2 cut(s)	77, 190
XspI	CTAG	9 cut(s)	240, 261, 282, 483, 798, 828, 1200, 2255, 2615
ZraI	GACGTC	1 cut(s)	1997

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.