RLG00000025275

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002897
Physical Location & Seq
Reverse (-)
43526275 .. 43539682
13408 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025275

Sequence Viewer

Length: 4593 bp
ATGGGCGGTCACGATCTGGAAATGGAGAAAGCTCGACTGTTAAGTCTGGCTCTTAATTTTGGGTTCGATGAAGAATCGGCCAACAAGTGCTTGGATCGTATGGTTGATCTCTACGGTGAAGATGGCCTAGATTTTATTACTGTGGAGCATTGCGGTGATGAGTTTCTTTCACAACTAGCTGACTCCGTTCAAGACACTGAAGAATGGGATGATTTACAAGCAATGGAGTCTGAAGCTTGTGGAGCATTGAATAACATGTTTGGAAATGGTAATGAAGCAGGTAACGATGATAATGCTAGCGCCAAAACCAACATGCCAGAAGATTCACTTGAACGTCAAAAGTACCCAAATTTGTTGGTGCTGGATTCGAGTGATAGTGAAGACTTTGAATTAAGAACTCCATTAAGAAGAACAGTTGGATCCTTTTCTTCTTCATGTCCTGTTCAGAGCAATTCTGCCCTACCCCAGAATATGGAAAGATCAAGGGGTTGTGAAAGCACCATTAGTCGAGACAGAGTTCCATCAATTTCTAGTAAGATGCAGTGTGTTCGAACAGCAGAGGATGAATTTAAAACTCTTACTTATGAAGAACTACAGTTGCTGGATGATGCTGAGCTGGCCAATGTTGTAATATTTGGTAATAGGACCTTTCGATCATTGCAGCATCAGGCTTGTAAAGCAGCCATGGCAAAGCAAGATTGCTTTATCCTAATGCCTACTGGAGGGGGTAAAAGCTTATGTTACCAGCTTCCTGCAACCCTGAAACCAGGTGTGACAATAGTTGTCTCTCCACTGCTTTCTCTTATTCAGGATCAGATAGTAACTCTGAACCTCAAGTTTGGTATACCAGCAACTTTTTTGAATTCTCAGCAAACTGCTTCCCAAGCAGCAGCTGTCCTCCAAGAGCTGAGGCAAGATAAGCCATCATGCAAGCTTCTGTATGTGACTCCTGAGAGAATTGCTGGAAGCTCATGTTTCCTTGAGGTTCTAGAATGCTTGCATTTGAAGGGGATGCTAGCAGGATTTGTTGTTGATGAAGCGCACTGCGTAAGCCAATGGGGACATGATTTTCGTCCAGATTACAAGGGATTAGGTTGTCTTAAGCAGAAATTCCGAGATGTTCCGGTGATGGCATTGACTGCTACTGCAACCCATCCAGTTCGTAAGGACATACTAAAAATTTTGAAAATTCCCCATGCTCTTGTCCTTGAAAGAAGCTTTGACAGACCCAATTTGAAATATGAAGTGATTCGTAAGCCAAAGGAAGCACTAAAGCAGCTTGGGCAACTATTGATGAGCCGTTTTCAGAAGCAGTGTGGAATTATATACTGCCTCTCCAAAAGTGAATCCGTTGAGGTTTCAAAATTTCTGAATGAGAAATTTAAGATTAAGACAGCGCATTACCATGCCGGGATGGCAGCTCGGCAAAGAGTTGCTGTCCAAAAGAAATGGTACACAGGAGAGATCCAAATTGTATGTGCTACAATTGCTTTTGGGATGGGAATAGACAAGCCAGATGTTCGTTTTGTTATACACAACAGTCTATCCAAATCCATTGAAAGCTACTATCAAGAATCTGGAAGGGCTGGCAGAGATGATCTTCCTTCAGCATGCATTGTCCTGTACCAGAAGAAGGATTTTAGTCGGGTTGTATGTATGTTGCGAAACGGACAGGGATTTAAAAGTCAAAGCTTCAAGGCAGCTATGGCTCAAGCTCAGAAAATGCAAGAGTATTGTGAATTAAAGGCTCAGTGTCGGAGACAAATAGTATCAGACATTAGTGTGCTAATTTGGTCCATGGTGTCTGCATATGATATTTTTTATACAATGAATTTTGAGTGGACCTGTAGCATGAATATGAACAAGCAGCAAAGAAAAGCTTTGAAGTTGGCAACATGCTTAAGCTTTAGAGACGACGACGCCAGTGATGCCGAAGATGACGAGACGAAGTCGGTGCTGGAAAACAAAGGGGAAGGAGGCCGACGGAGGGATTTGTGCGGAGAAGAAGATGGAGGAATTTTAGATGAGAAAATGGAGGTTGAGAAAAAACGTTCAAAAGGGTTTTTCAATTTATTTGATTGGAATGGCAAATCTCGGAAGAAGCTCTTCTCCAGCAATTCTGAATCATCAGGATCACAGCAAGGAAAAGAGAATGTGGAGAATTTCTTGCAGACACGGCTCAATAGGGTTGAGGTGGATGAGAAAGGAGCAAGTTCAAGTAATAATGGAAGCAGCGATTGGAATTGTGCTTCGTCGGTGACTAGTGATGAAGGAGGTGGGAGTAGAGCCCCTGGGGTAGTTGCTAGATTAATGGGTCTTGATTCATTGCCAACTTCAACGGTTACTGATTCATCTTCTACCTTATCAATCGACTCCCACTCACGTAGAGTACCTCATCATGACAGGGTCAATCCTAATTTGTGGGGTGATTTTTATGCCATGGATTACATGAATATGCCCAACAAGCTGGATACAGTTTCAAGGAATCCTGTAGAATCTAGGGCACTGAGGGTGCAAAACCGGCCAATTGAGAGATTTCAAACAGAAGTCTTGCCTCCAAAATCAGCCAAGTCAATTCCAGTTACTCACCATAAGCTTTTGTCACCTATCAAGACTCCTGGCTTCATCCCAACAAAGAATGCGGCATACATAATGGAAGCAGCTGCAAAGATGATTGAGACAAGTCCTAGAGAATCTTCCAAGAGCGAAATGTCATCTATGAGATCGTCTTCAATTCCATTGAGAATTCGGGATTTGAAAGAGAAGATGGAAGCTGTGCCTAAAGTATCAAGGCCTGAACAACCCAAGGGACCTAGTGATGCTAAATATATGAAAGGAAGGCCTGGTTACAGGAGCTATAATGGGTCAGATAATGTACCAGCACCCAAGGCTTCTGTGGATTCAGAAAAACAGAATTACAATGATATAAGGAATAAGGGAAAAGCAGTACCACTTGCAGTACAAGCAAAGGCCAATGTTCAGAGAAAGGAAGGGTCCCCTCCTCTCAGTAATAGGAGTTTCGCAAACCAGAAGGAACAGAATGAGGTCAAACAAATTGAGTTCTCCAAGAGCCGGCAAAGCACACAGAGACCTGTGCATAAGAGAACTTCTGCTGTCAGCAACAAAAGTGTTCTCAAGCAGAACAACCAGAAGCAGAATTGTGTATCTAACAAAGATAAAATGACTTCAAAGAATGTGGTTCCTAATCAACCTACCAGAAGAATGCGGTCAACTAATGGCTCTAGTCGGCCAAATAGGACTGTAAATAAGGTTCTTATAAACTCTGAAACTGGATCAAGAAAGATGGGCTCAGTGGCAACTGCTACTGGAAAGGACTTCTCATTCTCCACAGTTAAAGATGTGTCTGGGAAGATACGGTCTGCTAGTCAGGACTTTCATTTAGAAGAAACTGCTGCTGATAATGGATTGATCGGTGAGGATGAAAGGTCTGTAAAATGTAATGTTGCAATGGAGGGGTACACCAATTTGTGCATGGATAATAGAAAACAAGACATGGATGTTGTTTCATTTACATTCACGTCTCCCCTGAAAAAATCCATTTCTGAATTGCAGTCCACTGGCCAAGTTCTTCAAGAATTGACGTGCAAAGTTGAGTCATCTCAGCATAACCTGTTTGGTGAAGGGACTTCTGCTAGTTCTTCGTCTAGCTTGCAAGATTTGGTTTCCAGTGAGGCAAGCACCACATCAAGAGGCAAGAAGTTCGAACTTGGTTTGCTCAGAGATAAAGTTGATGACATAGCGGACTTTGATTTTCCGTCACCTGATGTAAACCAAAAGTGGCAGGGACCAGAAGGGATGGAAGAGTGTAGCAGTAGCAGCAAAAACAGTGGAACTGGAAAGGATTTTGATTATCATCTTAACCCAGTTTCAGTTTTTGAACCCTCACTTGAGAGTGGAAGCTGCACAGATAACAGAAGCAGTGCCAATGGGTGTGAGAGTGAGAGGAGTTCATTTGCTCAAGCTCAAGATCAAATCAATTTATTCTCCACATTTGAAATCCAACCTTGTGGTGCTTCCGAGTTATCTGACTCGGCCTCCACTTGGGAAGTGAGTGGAAAAAATATGACTAGAATATTTGGTTTTCATTCTCCCAATCAATCAAACGATTGGGAACGAGAATATGTGCAGTATGTACTTAGCAAGGTGGACTTAGTGATTGAAGATTTTGCATTGGCTGATGCCGACTATGCTATTTCCCCAAATCTCTTTGATAATTTACACTATCAAGAAGAGTATCCGAAGCTCCAGCAGAAGCTCTTGTTTGACAGTGTAAACGAAAGCCTGCACAACAGATGCAAGCAAATTGTTGTTGGAAGCCGGAAAGCATGGGATAGATGGGAGACATTGTCGCAGAGGAAGGAATCGTTGGCTGAGGAACTGTATAAAGAGATTTCGGTTTGGAAAAATATGGGGGACTTGATGGCCGATGACCTTGTCGACAAGGACATGAGCACTCAGCATGGGAGATGGCTCGACTTTGAGATTGAAGCATTTGAGGAAGCTGTAGAGATTGAGAAGGGAATACTAGGTTGTTTAGTAGATGAGTTAGTATCTGATTTTCTGCTGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

1531

Amino Acids

170.88

Weight (kDa)

6.03

Isoelectric Point (pI)

53.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 219 - 387 3.1e-22 DEAD/DEAH box helicase
Helicase_C PF00271 424 - 531 1.7e-18 Helicase conserved C-terminal domain
VARLMGL PF14383 751 - 781 3e-14 DUF761-associated sequence motif
DUF4378 PF14309 1379 - 1522 8.1e-29 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3278
AasI GACNNNNNNGTC 1 cut(s) 42
Acc36I ACCTGC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 472
AccI GTMKAC 2 cut(s) 844, 4458
AciI CCGC 6 cut(s) 6, 153, 1998, 2642, 3226, 3761
AclI AACGTT 1 cut(s) 2050
AclWI GGATC 7 cut(s) 102, 414, 427, 819, 1459, 2140, 3301
AcoI YGGCCR 6 cut(s) 78, 618, 2522, 3248, 3580, 4443
AcuI CTGAAG 3 cut(s) 219, 252, 1590
AcyI GRCGYC 1 cut(s) 1920
AfaI GTAC 9 cut(s) 344, 1454, 1625, 2391, 2877, 2949, 2961, 3479, 4155
AfiI CCNNNNNNNGG 6 cut(s) 472, 722, 1633, 1987, 3072, 4062
AflII CTTAAG 2 cut(s) 1100, 1900
AflIII ACRYGT 1 cut(s) 255
AhlI ACTAGT 1 cut(s) 2261
AjiI CACGTC 2 cut(s) 3540, 3603
AjnI CCWGG 4 cut(s) 766, 2290, 2617, 2842
AjuI GAANNNNNNNTTGG 4 cut(s) 2221, 2253, 4370, 4402
Alw21I GWGCWC 1 cut(s) 4475
Alw26I GTCTC 9 cut(s) 504, 790, 1753, 1905, 1937, 2672, 3082, 3546, 4355
AlwI GGATC 7 cut(s) 102, 414, 427, 819, 1459, 2140, 3301
AlwNI CAGNNNCTG 2 cut(s) 601, 893
ArsI GACNNNNNNTTYG 2 cut(s) 1053, 1085
AseI ATTAAT 1 cut(s) 2309
Asp700I GAANNNNTTC 2 cut(s) 1248, 2105
AspLEI GCGC 3 cut(s) 302, 1042, 1399
AspS9I GGNCC 6 cut(s) 645, 1794, 1842, 2810, 2994, 3806
AsuC2I CCSGG 1 cut(s) 1411
AsuII TTCGAA 2 cut(s) 550, 3723
AsuNHI GCTAGC 2 cut(s) 296, 1015
AvaII GGWCC 6 cut(s) 645, 1794, 1842, 2810, 2994, 3806
BaeGI GKGCMC 1 cut(s) 2506
BalI TGGCCA 2 cut(s) 620, 3582
BamHI GGATCC 1 cut(s) 419
BanII GRGCYC 2 cut(s) 2290, 3311
BbsI GAAGAC 2 cut(s) 387, 2721
Bbv12I GWGCWC 1 cut(s) 4475
BbvCI CCTCAGC 2 cut(s) 908, 4392
BceAI ACGGC 2 cut(s) 1284, 2192
BcgI CGANNNNNNTGC 8 cut(s) 275, 309, 530, 564, 1936, 1970, 3703, 3737
BciT130I CCWGG 4 cut(s) 768, 2292, 2619, 2844
BciVI GTATCC 2 cut(s) 2464, 4266
BcnI CCSGG 1 cut(s) 1411
BcoDI GTCTC 9 cut(s) 504, 790, 1753, 1905, 1937, 2672, 3082, 3546, 4355
BcuI ACTAGT 1 cut(s) 2261
BfmI CTRYAG 4 cut(s) 593, 1846, 2490, 4524
BfoI RGCGCY 1 cut(s) 303
BfrI CTTAAG 2 cut(s) 1100, 1900
BfuAI ACCTGC 1 cut(s) 269
BfuI GTATCC 2 cut(s) 2464, 4266
BglI GCCNNNNNGGC 1 cut(s) 1415
BlpI GCTNAGC 1 cut(s) 612
Bme1390I CCNGG 5 cut(s) 768, 1411, 2292, 2619, 2844
Bme18I GGWCC 6 cut(s) 645, 1794, 1842, 2810, 2994, 3806
BmgBI CACGTC 2 cut(s) 3540, 3603
BmgT120I GGNCC 6 cut(s) 645, 1794, 1842, 2810, 2994, 3806
BmiI GGNNCC 6 cut(s) 421, 2811, 2995, 2996, 3201, 3807
BmrFI CCNGG 5 cut(s) 768, 1411, 2292, 2619, 2844
BmrI ACTGGG 1 cut(s) 3878
BmsI GCATC 8 cut(s) 528, 598, 673, 1002, 1918, 2809, 4189, 4304
BmtI GCTAGC 2 cut(s) 300, 1019
BmuI ACTGGG 1 cut(s) 3878
BpiI GAAGAC 2 cut(s) 387, 2721
BpmI CTGGAG 3 cut(s) 741, 2095, 4250
Bpu10I CCTNAGC 2 cut(s) 908, 4392
Bpu1102I GCTNAGC 1 cut(s) 612
Bpu14I TTCGAA 2 cut(s) 550, 3723
BpuEI CTTGAG 7 cut(s) 818, 1001, 1695, 3119, 3929, 3963, 3969
BpuMI CCSGG 1 cut(s) 1411
BsaAI YACGTR 1 cut(s) 2384
BsaBI GATNNNNATC 1 cut(s) 3873
BsaHI GRCGYC 1 cut(s) 1920
BsaI GGTCTC 1 cut(s) 3082
BsaJI CCNNGG 7 cut(s) 684, 1797, 2290, 2291, 2439, 2805, 2886
BsaWI WCCGGW 1 cut(s) 1123
BsaXI ACNNNNNCTCC 6 cut(s) 1319, 1349, 4040, 4070, 4352, 4382
Bsc4I CCNNNNNNNGG 6 cut(s) 472, 722, 1633, 1987, 3072, 4062
Bse118I RCCGGY 2 cut(s) 2520, 3072
Bse3DI GCAATG 5 cut(s) 148, 228, 656, 2324, 3474
Bse8I GATNNNNATC 1 cut(s) 3873
BseBI CCWGG 4 cut(s) 768, 2292, 2619, 2844
BseDI CCNNGG 7 cut(s) 684, 1797, 2290, 2291, 2439, 2805, 2886
BseJI GATNNNNATC 1 cut(s) 3873
BseLI CCNNNNNNNGG 6 cut(s) 472, 722, 1633, 1987, 3072, 4062
BseMI GCAATG 5 cut(s) 148, 228, 656, 2324, 3474
BseRI GAGGAG 2 cut(s) 2991, 3979
BseSI GKGCMC 1 cut(s) 2506
BsgI GTGCAG 3 cut(s) 3907, 4166, 4289
BsiHKAI GWGCWC 1 cut(s) 4475
BsiSI CCGG 5 cut(s) 1124, 1410, 2521, 3073, 4339
BslFI GGGAC 6 cut(s) 1074, 2823, 2980, 3658, 3819, 4448
BslI CCNNNNNNNGG 6 cut(s) 472, 722, 1633, 1987, 3072, 4062
BsmAI GTCTC 9 cut(s) 504, 790, 1753, 1905, 1937, 2672, 3082, 3546, 4355
BsmBI CGTCTC 3 cut(s) 1905, 1937, 3546
BsmFI GGGAC 6 cut(s) 1074, 2823, 2980, 3658, 3819, 4448
BsmI GAATGC 3 cut(s) 998, 2644, 3228
Bso31I GGTCTC 1 cut(s) 3082
Bsp119I TTCGAA 2 cut(s) 550, 3723
Bsp1286I GDGCHC 4 cut(s) 2290, 2506, 3311, 4475
Bsp1720I GCTNAGC 1 cut(s) 612
Bsp19I CCATGG 3 cut(s) 684, 1797, 2439
BspACI CCGC 6 cut(s) 6, 153, 1998, 2642, 3226, 3761
BspHI TCATGA 1 cut(s) 2398
BspLI GGNNCC 6 cut(s) 421, 2811, 2995, 2996, 3201, 3807
BspMI ACCTGC 1 cut(s) 269
BspOI GCTAGC 2 cut(s) 300, 1019
BspPI GGATC 7 cut(s) 102, 414, 427, 819, 1459, 2140, 3301
BspQI GCTCTTC 1 cut(s) 2111
BspT104I TTCGAA 2 cut(s) 550, 3723
BspTI CTTAAG 2 cut(s) 1100, 1900
BspTNI GGTCTC 1 cut(s) 3082
BsrDI GCAATG 5 cut(s) 148, 228, 656, 2324, 3474
BsrFI RCCGGY 2 cut(s) 2520, 3072
BssAI RCCGGY 2 cut(s) 2520, 3072
BssECI CCNNGG 7 cut(s) 684, 1797, 2290, 2291, 2439, 2805, 2886
BssNAI GTATAC 1 cut(s) 845
BssNI GRCGYC 1 cut(s) 1920
BssT1I CCWWGG 5 cut(s) 684, 1797, 2439, 2805, 2886
Bst1107I GTATAC 1 cut(s) 845
Bst2UI CCWGG 4 cut(s) 768, 2292, 2619, 2844
Bst6I CTCTTC 3 cut(s) 2111, 3816, 4245
BstACI GRCGYC 1 cut(s) 1920
BstAFI CTTAAG 2 cut(s) 1100, 1900
BstAPI GCANNNNNTGC 1 cut(s) 1139
BstBAI YACGTR 1 cut(s) 2384
BstBI TTCGAA 2 cut(s) 550, 3723
BstDSI CCRYGG 3 cut(s) 684, 1797, 2439
BstENI CCTNNNNNAGG 1 cut(s) 720
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 3 cut(s) 302, 1042, 1399
BstMAI GTCTC 9 cut(s) 504, 790, 1753, 1905, 1937, 2672, 3082, 3546, 4355
BstNI CCWGG 4 cut(s) 768, 2292, 2619, 2844
BstNSI RCATGY 4 cut(s) 259, 316, 1614, 1899
BstSCI CCNGG 5 cut(s) 766, 1409, 2290, 2617, 2842
BstSFI CTRYAG 4 cut(s) 593, 1846, 2490, 4524
BstSLI GKGCMC 1 cut(s) 2506
BstV2I GAAGAC 2 cut(s) 387, 2721
BstX2I RGATCY 2 cut(s) 419, 1464
BstXI CCANNNNNNTGG 2 cut(s) 2467, 4028
BstYI RGATCY 2 cut(s) 419, 1464
BstZ17I GTATAC 1 cut(s) 845
BsuI GTATCC 2 cut(s) 2464, 4266
BtgI CCRYGG 3 cut(s) 684, 1797, 2439
BtrI CACGTC 2 cut(s) 3540, 3603
BtsI GCAGTG 5 cut(s) 548, 791, 1042, 1319, 3946
BveI ACCTGC 1 cut(s) 269
CaiI CAGNNNCTG 2 cut(s) 601, 893
CciI TCATGA 1 cut(s) 2398
CfoI GCGC 3 cut(s) 302, 1042, 1399
Cfr10I RCCGGY 2 cut(s) 2520, 3072
Cfr13I GGNCC 6 cut(s) 645, 1794, 1842, 2810, 2994, 3806
CseI GACGC 1 cut(s) 1928
CsiI ACCWGGT 1 cut(s) 766
Csp6I GTAC 9 cut(s) 343, 1453, 1624, 2390, 2876, 2948, 2960, 3478, 4154
CspCI CAANNNNNGTGG 4 cut(s) 3177, 3212, 3829, 3864
CviQI GTAC 9 cut(s) 343, 1453, 1624, 2390, 2876, 2948, 2960, 3478, 4154
DraI TTTAAA 2 cut(s) 571, 1681
DrdI GACNNNNNNGTC 1 cut(s) 42
DseDI GACNNNNNNGTC 1 cut(s) 42
EaeI YGGCCR 6 cut(s) 78, 618, 2522, 3248, 3580, 4443
Eam1104I CTCTTC 3 cut(s) 2111, 3816, 4245
EarI CTCTTC 3 cut(s) 2111, 3816, 4245
Eco130I CCWWGG 5 cut(s) 684, 1797, 2439, 2805, 2886
Eco147I AGGCCT 2 cut(s) 2794, 2842
Eco24I GRGCYC 2 cut(s) 2290, 3311
Eco31I GGTCTC 1 cut(s) 3082
Eco47I GGWCC 6 cut(s) 645, 1794, 1842, 2810, 2994, 3806
Eco57I CTGAAG 3 cut(s) 219, 252, 1590
EcoNI CCTNNNNNAGG 1 cut(s) 720
EcoO109I RGGNCCY 3 cut(s) 645, 2810, 2994
EcoRI GAATTC 2 cut(s) 862, 2745
EcoRII CCWGG 4 cut(s) 766, 2290, 2617, 2842
EcoT14I CCWWGG 5 cut(s) 684, 1797, 2439, 2805, 2886
EcoT22I ATGCAT 1 cut(s) 1616
EcoT38I GRGCYC 2 cut(s) 2290, 3311
ErhI CCWWGG 5 cut(s) 684, 1797, 2439, 2805, 2886
Esp3I CGTCTC 3 cut(s) 1905, 1937, 3546
FalI AAGNNNNNCTT 8 cut(s) 312, 344, 1864, 1896, 2090, 2122, 4154, 4186
FaqI GGGAC 6 cut(s) 1074, 2823, 2980, 3658, 3819, 4448
FauNDI CATATG 1 cut(s) 1810
FblI GTMKAC 2 cut(s) 844, 4458
FriOI GRGCYC 2 cut(s) 2290, 3311
GlaI GCGC 3 cut(s) 301, 1041, 1398
GsuI CTGGAG 3 cut(s) 741, 2095, 4250
HaeII RGCGCY 1 cut(s) 303
HapII CCGG 5 cut(s) 1124, 1410, 2521, 3073, 4339
HgaI GACGC 1 cut(s) 1928
HhaI GCGC 3 cut(s) 302, 1042, 1399
Hin1I GRCGYC 1 cut(s) 1920
Hin6I GCGC 3 cut(s) 300, 1040, 1397
HinP1I GCGC 3 cut(s) 300, 1040, 1397
HincII GTYRAC 2 cut(s) 3231, 4459
HindII GTYRAC 2 cut(s) 3231, 4459
HindIII AAGCTT 8 cut(s) 234, 733, 932, 1216, 1690, 1878, 1903, 2594
HpaII CCGG 5 cut(s) 1124, 1410, 2521, 3073, 4339
Hpy99I CGWCG 4 cut(s) 1919, 1922, 1986, 2257
HpyCH4IV ACGT 5 cut(s) 334, 2050, 2383, 3539, 3602
HpySE526I ACGT 5 cut(s) 334, 2050, 2383, 3539, 3602
Hsp92I GRCGYC 1 cut(s) 1920
HspAI GCGC 3 cut(s) 300, 1040, 1397
KflI GGGWCCC 1 cut(s) 2994
KroI GCCGGC 1 cut(s) 3072
KroNI GCCGGC 1 cut(s) 3074
LguI GCTCTTC 1 cut(s) 2111
LmnI GCTCC 5 cut(s) 145, 242, 2207, 2853, 4269
LweI GCATC 8 cut(s) 528, 598, 673, 1002, 1918, 2809, 4189, 4304
MabI ACCWGGT 1 cut(s) 766
MaeII ACGT 5 cut(s) 334, 2050, 2383, 3539, 3602
MfeI CAATTG 2 cut(s) 1485, 2526
MflI RGATCY 2 cut(s) 419, 1464
MhlI GDGCHC 4 cut(s) 2290, 2506, 3311, 4475
MlsI TGGCCA 2 cut(s) 620, 3582
MluNI TGGCCA 2 cut(s) 620, 3582
MlyI GAGTC 7 cut(s) 176, 236, 940, 2366, 2608, 3623, 4043
MmeI TCCRAC 4 cut(s) 397, 1736, 4045, 4312
Mox20I TGGCCA 2 cut(s) 620, 3582
Mph1103I ATGCAT 1 cut(s) 1616
MroNI GCCGGC 1 cut(s) 3072
MroXI GAANNNNTTC 2 cut(s) 1248, 2105
MscI TGGCCA 2 cut(s) 620, 3582
MslI CAYNNNNRTG 5 cut(s) 153, 1404, 1781, 1856, 2453
Msp20I TGGCCA 2 cut(s) 620, 3582
MspA1I CMGCKG 2 cut(s) 893, 2663
MspCI CTTAAG 2 cut(s) 1100, 1900
MspI CCGG 5 cut(s) 1124, 1410, 2521, 3073, 4339
MspR9I CCNGG 5 cut(s) 768, 1411, 2292, 2619, 2844
MunI CAATTG 2 cut(s) 1485, 2526
Mva1269I GAATGC 3 cut(s) 998, 2644, 3228
MvaI CCWGG 4 cut(s) 768, 2292, 2619, 2844
NaeI GCCGGC 1 cut(s) 3074
NciI CCSGG 1 cut(s) 1411
NcoI CCATGG 3 cut(s) 684, 1797, 2439
NdeI CATATG 1 cut(s) 1810
NgoMIV GCCGGC 1 cut(s) 3072
NheI GCTAGC 2 cut(s) 296, 1015
NlaIV GGNNCC 6 cut(s) 421, 2811, 2995, 2996, 3201, 3807
NmeAIII GCCGAG 2 cut(s) 1402, 4031
NmuCI GTSAC 6 cut(s) 8, 772, 943, 2257, 2601, 3777
NsiI ATGCAT 1 cut(s) 1616
NspI RCATGY 4 cut(s) 259, 316, 1614, 1899
NspV TTCGAA 2 cut(s) 550, 3723
PaeI GCATGC 1 cut(s) 1614
PagI TCATGA 1 cut(s) 2398
PasI CCCWGGG 1 cut(s) 2291
PceI AGGCCT 2 cut(s) 2794, 2842
PciI ACATGT 1 cut(s) 255
PciSI GCTCTTC 1 cut(s) 2111
PctI GAATGC 3 cut(s) 998, 2644, 3228
PdiI GCCGGC 1 cut(s) 3074
PdmI GAANNNNTTC 2 cut(s) 1248, 2105
PflFI GACNNNGTC 4 cut(s) 1948, 2405, 4366, 4454
PflMI CCANNNNNTGG 1 cut(s) 472
PleI GAGTC 7 cut(s) 176, 235, 940, 2366, 2608, 3622, 4043
PpsI GAGTC 7 cut(s) 176, 235, 940, 2366, 2608, 3622, 4043
Ppu21I YACGTR 1 cut(s) 2384
PpuMI RGGWCCY 3 cut(s) 645, 2810, 2994
PscI ACATGT 1 cut(s) 255
PshBI ATTAAT 1 cut(s) 2309
PsiI TTATAA 1 cut(s) 3278
Psp1406I AACGTT 1 cut(s) 2050
Psp5II RGGWCCY 3 cut(s) 645, 2810, 2994
Psp6I CCWGG 4 cut(s) 766, 2290, 2617, 2842
PspGI CCWGG 4 cut(s) 766, 2290, 2617, 2842
PspN4I GGNNCC 6 cut(s) 421, 2811, 2995, 2996, 3201, 3807
PspPI GGNCC 6 cut(s) 645, 1794, 1842, 2810, 2994, 3806
PspPPI RGGWCCY 3 cut(s) 645, 2810, 2994
PsrI GAACNNNNNNTAC 2 cut(s) 3255, 3287
PstNI CAGNNNCTG 2 cut(s) 601, 893
PsuI RGATCY 2 cut(s) 419, 1464
PsyI GACNNNGTC 4 cut(s) 1948, 2405, 4366, 4454
PvuII CAGCTG 2 cut(s) 893, 2663
RsaI GTAC 9 cut(s) 344, 1454, 1625, 2391, 2877, 2949, 2961, 3479, 4155
RsaNI GTAC 9 cut(s) 343, 1453, 1624, 2390, 2876, 2948, 2960, 3478, 4154
RseI CAYNNNNRTG 5 cut(s) 153, 1404, 1781, 1856, 2453
SalI GTCGAC 1 cut(s) 4457
SapI GCTCTTC 1 cut(s) 2111
Sau96I GGNCC 6 cut(s) 645, 1794, 1842, 2810, 2994, 3806
SchI GAGTC 7 cut(s) 176, 236, 940, 2366, 2608, 3623, 4043
ScrFI CCNGG 5 cut(s) 768, 1411, 2292, 2619, 2844
SduI GDGCHC 4 cut(s) 2290, 2506, 3311, 4475
SexAI ACCWGGT 1 cut(s) 766
SfaNI GCATC 8 cut(s) 528, 598, 673, 1002, 1918, 2809, 4189, 4304
SfcI CTRYAG 4 cut(s) 593, 1846, 2490, 4524
SfuI TTCGAA 2 cut(s) 550, 3723
SinI GGWCC 6 cut(s) 645, 1794, 1842, 2810, 2994, 3806
SmiMI CAYNNNNRTG 5 cut(s) 153, 1404, 1781, 1856, 2453
SmlI CTYRAG 9 cut(s) 833, 980, 1100, 1710, 1900, 3134, 3908, 3978, 3984
SmoI CTYRAG 9 cut(s) 833, 980, 1100, 1710, 1900, 3134, 3908, 3978, 3984
SpeI ACTAGT 1 cut(s) 2261
SphI GCATGC 1 cut(s) 1614
SseBI AGGCCT 2 cut(s) 2794, 2842
SsiI CCGC 6 cut(s) 6, 153, 1998, 2642, 3226, 3761
SspI AATATT 2 cut(s) 633, 4095
StuI AGGCCT 2 cut(s) 2794, 2842
StyD4I CCNGG 5 cut(s) 766, 1409, 2290, 2617, 2842
StyI CCWWGG 5 cut(s) 684, 1797, 2439, 2805, 2886
TaiI ACGT 5 cut(s) 337, 2053, 2386, 3542, 3605
TatI WGTACW 2 cut(s) 2959, 4153
TauI GCSGC 1 cut(s) 2645
TseFI GTSAC 6 cut(s) 8, 772, 943, 2257, 2601, 3777
Tsp45I GTSAC 6 cut(s) 8, 772, 943, 2257, 2601, 3777
TspGWI ACGGA 5 cut(s) 175, 1339, 1683, 1999, 3765
Tth111I GACNNNGTC 4 cut(s) 1948, 2405, 4366, 4454
Van91I CCANNNNNTGG 1 cut(s) 472
Vha464I CTTAAG 2 cut(s) 1100, 1900
VpaK11BI GGWCC 6 cut(s) 645, 1794, 1842, 2810, 2994, 3806
VspI ATTAAT 1 cut(s) 2309
XagI CCTNNNNNAGG 1 cut(s) 720
XbaI TCTAGA 1 cut(s) 988
XceI RCATGY 4 cut(s) 259, 316, 1614, 1899
XcmI CCANNNNNNNNNTGG 3 cut(s) 88, 2893, 3490
XmiI GTMKAC 2 cut(s) 844, 4458
XmnI GAANNNNTTC 2 cut(s) 1248, 2105
Zsp2I ATGCAT 1 cut(s) 1616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.