Rw3G006560

Belongs to the helicase family. RecQ subfamily

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
Physical Location & Seq
Forward (+)
6169098 .. 6173312
4215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G006560.1

Sequence Viewer

Length: 1809 bp
ATGGGCGGCCACGATCTGGAAATGGAGAAAGCTCGACTCTTAAGTTTGGCTCTTAATTTTGGGTTCGATGAAGAATCGGCCAACAAGTGCTTGGATCGTATGGTTGATCTCTACGGTGAAGATGGCCTAGATTTTATTACTGTGGAGCATTGCGGTGATGAGTTTCTTTCACAACTAGCTGAATCCGTTCAAGACACTGAAGAATGGGATGATTTACAAGCAATGGAGTCTGAAGCTTGTGGAGCATTGAATAACATGTTTGGAAATGGTAATGAAGCAGGTAACTATGATAATGCTAGTGCCAAAACCAACATGCCAGAAGATTCACTTGAACGTCAAAAGTACCCAAATTTGTTGGTGCTGGATTCGAGTGATAGTGAAGACTTTGAATTAAGAACTCCATTAAGAAGAACAGTTGGATCCTTTTCTTCTTCATGTCCTGGTTGTGAAAGTACCATTAGTCGAGACAGAGTTCCATCAATTTCTAGTAAGAAGCAGTGTGTTCGAACAGCAAAGGATGAATTTAAGTCTCTTACTTATGAAGAACTACAGTTGCTGGATGATGCTGAGCTGGCCAATGTTGTAATATTTGGTAATAGGACCTTTCGACCATTGCAGCATCAGGCTTGCAAAGCAGCCATGGCAAAGCAAGATTGCTTTATCCTAATGCCTACTGGAGGGGGTAAAAGCTTATGTTACCAGCTTCCTGCAACCCTGAAACCAGGTGTGACAATAGTTGTCTCTCCACTGCTTTCTCTTATTCAGGATCAGATAGTAACTCTGAACCTCAAGTTTGGTATACCAGCAACTTTTTTGAATTCTCAGCAAACTGCTTCCCAAGCAGCAGCTGTCCTCCAAGAGCTGAGGAAAGATAAGCCATCATGCAAGCTTCTGTATGTGACTCCTGAGAGAATTGCTGGAAGCTCATGTTTCCTTGAGGTTCTAGAATGCTTGCATTTGAAGGGGATGCTAGCAGGATTTGTTGTTGATGAAGCACACTGCGTAAGCCAATGGGGACATGATTTTCGTCCAGATTACAAGGGATTAGGTTGTCTTAAGCAGAAGTTCCGAGATGTTCCGGTGATGGCATTGACTGCTACTGCAACCCATCCAGTTCGTAAGGACATACTAAAAATTTTGAAAATTCCCCATGCTCTTGTCCTTGAAAGAAGCTTTGACAGACCCAATTTGAAATATGAAGTGATTCGTAAGGCGAAGGAAGCACTAAAGCAGCTTGGGCAACTATTGATGAGCCGTTTTCAGAACCAGTGTGGAATTATATACTGCCTCTCCAAAAGTGAATCCGTTGAGGTTTCAAAATTTCTGAATGAGAAATTTAAGATTAAGACAGCGCATTACCATGCCGGGATGGCAGCTCGCCAAAGAGTTGCTGTCCAAAAGAAATGGTACACAGGAGAGATCCAAATTGTATGTGCTACAATTGCTTTTGGGATGGGAATAGACAAGCCGGATGTTCGTTTTGTTATACACAACAGTCTATCCAAATCCATTGAAAGCTACTATCAAGAATCTGGAAGGGCTGGCAGAGATGATCTTCCTTCAGCATGCATTGTCCTGTACCAGAAGAAGGATTTTAGTCGGGTTGTATGTATGTTGCGAAACGGACAAGGATTTAAAAGTCAAAGCTTCAAGGCAGCTATGGCTCAAGCTCAGAAAATGCAGGAGTATTGTGAATTAAAGGCTCAGTGTCGGAGACAAATGTTGCTCAAACACTTCGGAGAATCTTTTGATGAAAACGCCTGCAAAAATGGATCTAATCCTTGTGATAACTGTCTCAAGACTTCCTAG

Protein Analysis

602

Amino Acids

67.26

Weight (kDa)

6.85

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 203 - 372 2.3e-23 DEAD/DEAH box helicase
Helicase_C PF00271 409 - 516 3.8e-19 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 530 - 599 1.6e-14 RecQ zinc-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 16
AccI GTMKAC 1 cut(s) 799
AciI CCGC 2 cut(s) 6, 153
AclWI GGATC 6 cut(s) 102, 414, 427, 774, 1414, 1780
AcoI YGGCCR 3 cut(s) 7, 78, 573
AcsI RAATTY 7 cut(s) 349, 521, 817, 1134, 1143, 1319, 1334
AcuI CTGAAG 3 cut(s) 219, 252, 1545
AfaI GTAC 4 cut(s) 344, 454, 1409, 1580
AfiI CCNNNNNNNGG 3 cut(s) 16, 677, 1588
AflII CTTAAG 2 cut(s) 40, 1055
AflIII ACRYGT 1 cut(s) 255
AjnI CCWGG 2 cut(s) 439, 721
Alw26I GTCTC 5 cut(s) 459, 534, 745, 1708, 1799
AlwI GGATC 6 cut(s) 102, 414, 427, 774, 1414, 1780
AlwNI CAGNNNCTG 2 cut(s) 556, 848
AoxI GGCC 4 cut(s) 7, 78, 124, 573
ApeKI GCWGC 7 cut(s) 616, 635, 842, 845, 1231, 1373, 1655
ApoI RAATTY 7 cut(s) 349, 521, 817, 1134, 1143, 1319, 1334
ArsI GACNNNNNNTTYG 2 cut(s) 1008, 1040
Asp700I GAANNNNTTC 2 cut(s) 186, 1203
AspLEI GCGC 1 cut(s) 1354
AspS9I GGNCC 1 cut(s) 600
AsuC2I CCSGG 1 cut(s) 1366
AsuHPI GGTGA 3 cut(s) 128, 167, 1093
AsuII TTCGAA 1 cut(s) 505
AsuNHI GCTAGC 1 cut(s) 970
AvaII GGWCC 1 cut(s) 600
BalI TGGCCA 1 cut(s) 575
BamHI GGATCC 1 cut(s) 419
BbsI GAAGAC 1 cut(s) 387
BbvCI CCTCAGC 1 cut(s) 863
BbvI GCAGC 7 cut(s) 628, 647, 854, 857, 1243, 1385, 1667
BccI CCATC 7 cut(s) 116, 484, 886, 1078, 1116, 1363, 1447
BceAI ACGGC 1 cut(s) 1239
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BciT130I CCWGG 2 cut(s) 441, 723
BcnI CCSGG 1 cut(s) 1366
BcoDI GTCTC 5 cut(s) 459, 534, 745, 1708, 1799
BfaI CTAG 7 cut(s) 128, 176, 297, 486, 944, 971, 1807
BfmI CTRYAG 1 cut(s) 548
BfrI CTTAAG 2 cut(s) 40, 1055
BfuAI ACCTGC 1 cut(s) 269
BglI GCCNNNNNGGC 1 cut(s) 1370
BisI GCNGC 8 cut(s) 7, 617, 636, 843, 846, 1232, 1374, 1656
BlpI GCTNAGC 1 cut(s) 567
BlsI GCNGC 8 cut(s) 8, 618, 637, 844, 847, 1233, 1375, 1657
Bme1390I CCNGG 3 cut(s) 441, 723, 1366
Bme18I GGWCC 1 cut(s) 600
BmgT120I GGNCC 1 cut(s) 600
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 3 cut(s) 441, 723, 1366
BmsI GCATC 3 cut(s) 553, 628, 957
BmtI GCTAGC 1 cut(s) 974
BpiI GAAGAC 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 696
Bpu10I CCTNAGC 1 cut(s) 863
Bpu1102I GCTNAGC 1 cut(s) 567
Bpu14I TTCGAA 1 cut(s) 505
BpuEI CTTGAG 4 cut(s) 773, 956, 1650, 1781
BpuMI CCSGG 1 cut(s) 1366
BsaJI CCNNGG 1 cut(s) 639
BsaWI WCCGGW 1 cut(s) 1078
BsaXI ACNNNNNCTCC 2 cut(s) 1274, 1304
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 677, 1588
Bse1I ACTGG 3 cut(s) 679, 1112, 1267
Bse3DI GCAATG 3 cut(s) 148, 228, 611
BseBI CCWGG 2 cut(s) 441, 723
BseDI CCNNGG 1 cut(s) 639
BseGI GGATG 8 cut(s) 214, 523, 565, 972, 1108, 1374, 1458, 1477
BseLI CCNNNNNNNGG 3 cut(s) 16, 677, 1588
BseMI GCAATG 3 cut(s) 148, 228, 611
BseMII CTCAG 6 cut(s) 558, 836, 854, 897, 1685, 1718
BseNI ACTGG 3 cut(s) 679, 1112, 1267
BseXI GCAGC 7 cut(s) 628, 647, 854, 857, 1243, 1385, 1667
BshFI GGCC 4 cut(s) 9, 80, 126, 575
BsiSI CCGG 3 cut(s) 1079, 1365, 1469
BslFI GGGAC 1 cut(s) 1029
BslI CCNNNNNNNGG 3 cut(s) 16, 677, 1588
BsmAI GTCTC 5 cut(s) 459, 534, 745, 1708, 1799
BsmFI GGGAC 1 cut(s) 1029
BsmI GAATGC 1 cut(s) 953
BsnI GGCC 4 cut(s) 9, 80, 126, 575
Bsp119I TTCGAA 1 cut(s) 505
Bsp143I GATC 8 cut(s) 13, 94, 106, 419, 766, 1419, 1552, 1772
Bsp1720I GCTNAGC 1 cut(s) 567
Bsp19I CCATGG 1 cut(s) 639
BspACI CCGC 2 cut(s) 6, 153
BspANI GGCC 4 cut(s) 9, 80, 126, 575
BspCNI CTCAG 6 cut(s) 559, 835, 855, 898, 1684, 1717
BspLI GGNNCC 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 269
BspOI GCTAGC 1 cut(s) 974
BspPI GGATC 6 cut(s) 102, 414, 427, 774, 1414, 1780
BspT104I TTCGAA 1 cut(s) 505
BspTI CTTAAG 2 cut(s) 40, 1055
BsrDI GCAATG 3 cut(s) 148, 228, 611
BsrI ACTGG 3 cut(s) 679, 1112, 1267
BssECI CCNNGG 1 cut(s) 639
BssMI GATC 8 cut(s) 13, 94, 106, 419, 766, 1419, 1552, 1772
BssNAI GTATAC 1 cut(s) 800
BssT1I CCWWGG 1 cut(s) 639
Bst1107I GTATAC 1 cut(s) 800
Bst2UI CCWGG 2 cut(s) 441, 723
Bst4CI ACNGT 6 cut(s) 116, 142, 415, 552, 1496, 1793
BstAFI CTTAAG 2 cut(s) 40, 1055
BstAPI GCANNNNNTGC 1 cut(s) 1094
BstBI TTCGAA 1 cut(s) 505
BstC8I GCNNGC 9 cut(s) 573, 628, 887, 953, 972, 1378, 1543, 1567, 1762
BstDEI CTNAG 6 cut(s) 567, 822, 863, 906, 1671, 1704
BstDSI CCRYGG 1 cut(s) 639
BstENI CCTNNNNNAGG 1 cut(s) 675
BstF5I GGATG 8 cut(s) 214, 523, 565, 972, 1108, 1374, 1458, 1477
BstHHI GCGC 1 cut(s) 1354
BstKTI GATC 8 cut(s) 16, 97, 109, 422, 769, 1422, 1555, 1775
BstMAI GTCTC 5 cut(s) 459, 534, 745, 1708, 1799
BstMBI GATC 8 cut(s) 13, 94, 106, 419, 766, 1419, 1552, 1772
BstNI CCWGG 2 cut(s) 441, 723
BstNSI RCATGY 3 cut(s) 259, 316, 1569
BstSCI CCNGG 3 cut(s) 439, 721, 1364
BstSFI CTRYAG 1 cut(s) 548
BstV1I GCAGC 7 cut(s) 628, 647, 854, 857, 1243, 1385, 1667
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 3 cut(s) 419, 1419, 1772
BstYI RGATCY 3 cut(s) 419, 1419, 1772
BstZ17I GTATAC 1 cut(s) 800
BsuRI GGCC 4 cut(s) 9, 80, 126, 575
BtgI CCRYGG 1 cut(s) 639
BtsCI GGATG 8 cut(s) 214, 523, 565, 972, 1108, 1374, 1458, 1477
BtsI GCAGTG 3 cut(s) 503, 746, 997
BtsIMutI CAGTG 6 cut(s) 195, 503, 746, 997, 1274, 1712
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 9 cut(s) 573, 628, 887, 953, 972, 1378, 1543, 1567, 1762
CaiI CAGNNNCTG 2 cut(s) 556, 848
CfoI GCGC 1 cut(s) 1354
Cfr13I GGNCC 1 cut(s) 600
CsiI ACCWGGT 1 cut(s) 721
Csp6I GTAC 4 cut(s) 343, 453, 1408, 1579
CviQI GTAC 4 cut(s) 343, 453, 1408, 1579
DdeI CTNAG 6 cut(s) 567, 822, 863, 906, 1671, 1704
DpnI GATC 8 cut(s) 15, 96, 108, 421, 768, 1421, 1554, 1774
DpnII GATC 8 cut(s) 13, 94, 106, 419, 766, 1419, 1552, 1772
DraI TTTAAA 1 cut(s) 1636
EaeI YGGCCR 3 cut(s) 7, 78, 573
Eco130I CCWWGG 1 cut(s) 639
Eco47I GGWCC 1 cut(s) 600
Eco57I CTGAAG 3 cut(s) 219, 252, 1545
EcoNI CCTNNNNNAGG 1 cut(s) 675
EcoO109I RGGNCCY 1 cut(s) 600
EcoRI GAATTC 1 cut(s) 817
EcoRII CCWGG 2 cut(s) 439, 721
EcoT14I CCWWGG 1 cut(s) 639
EcoT22I ATGCAT 1 cut(s) 1571
ErhI CCWWGG 1 cut(s) 639
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 1 cut(s) 1029
FblI GTMKAC 1 cut(s) 799
Fnu4HI GCNGC 8 cut(s) 7, 617, 636, 843, 846, 1232, 1374, 1656
FokI GGATG 8 cut(s) 221, 530, 572, 979, 1095, 1381, 1465, 1484
Fsp4HI GCNGC 8 cut(s) 7, 617, 636, 843, 846, 1232, 1374, 1656
FspBI CTAG 7 cut(s) 128, 176, 297, 486, 944, 971, 1807
GlaI GCGC 1 cut(s) 1353
GluI GCNGC 8 cut(s) 7, 617, 636, 843, 846, 1232, 1374, 1656
GsuI CTGGAG 1 cut(s) 696
HaeIII GGCC 4 cut(s) 9, 80, 126, 575
HapII CCGG 3 cut(s) 1079, 1365, 1469
HhaI GCGC 1 cut(s) 1354
Hin6I GCGC 1 cut(s) 1352
HinP1I GCGC 1 cut(s) 1352
HindIII AAGCTT 5 cut(s) 234, 688, 887, 1171, 1645
HpaII CCGG 3 cut(s) 1079, 1365, 1469
HphI GGTGA 3 cut(s) 128, 167, 1093
Hpy166II GTNNAC 2 cut(s) 800, 1410
Hpy188I TCNGA 9 cut(s) 232, 771, 783, 1070, 1263, 1326, 1674, 1713, 1739
Hpy8I GTNNAC 2 cut(s) 800, 1410
HpyAV CCTTC 5 cut(s) 955, 1210, 1530, 1569, 1582
HpyCH4III ACNGT 6 cut(s) 116, 142, 415, 552, 1496, 1793
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 9 cut(s) 616, 630, 710, 885, 955, 1103, 1569, 1681, 1764
HpyF3I CTNAG 6 cut(s) 567, 822, 863, 906, 1671, 1704
HpySE526I ACGT 1 cut(s) 334
HspAI GCGC 1 cut(s) 1352
Kzo9I GATC 8 cut(s) 13, 94, 106, 419, 766, 1419, 1552, 1772
LmnI GCTCC 2 cut(s) 145, 242
Lsp1109I GCAGC 7 cut(s) 628, 647, 854, 857, 1243, 1385, 1667
LweI GCATC 3 cut(s) 553, 628, 957
MabI ACCWGGT 1 cut(s) 721
MaeI CTAG 7 cut(s) 128, 176, 297, 486, 944, 971, 1807
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 5 cut(s) 281, 695, 727, 775, 898
MalI GATC 8 cut(s) 15, 96, 108, 421, 768, 1421, 1554, 1774
MboI GATC 8 cut(s) 13, 94, 106, 419, 766, 1419, 1552, 1772
MfeI CAATTG 1 cut(s) 1440
MflI RGATCY 3 cut(s) 419, 1419, 1772
MlsI TGGCCA 1 cut(s) 575
MluNI TGGCCA 1 cut(s) 575
MlyI GAGTC 3 cut(s) 30, 236, 895
MmeI TCCRAC 2 cut(s) 397, 1691
MnlI CCTC 7 cut(s) 671, 797, 858, 863, 931, 1298, 1303
Mox20I TGGCCA 1 cut(s) 575
Mph1103I ATGCAT 1 cut(s) 1571
MroXI GAANNNNTTC 2 cut(s) 186, 1203
MscI TGGCCA 1 cut(s) 575
MslI CAYNNNNRTG 2 cut(s) 153, 1359
Msp20I TGGCCA 1 cut(s) 575
MspA1I CMGCKG 1 cut(s) 848
MspCI CTTAAG 2 cut(s) 40, 1055
MspI CCGG 3 cut(s) 1079, 1365, 1469
MspR9I CCNGG 3 cut(s) 441, 723, 1366
MunI CAATTG 1 cut(s) 1440
Mva1269I GAATGC 1 cut(s) 953
MvaI CCWGG 2 cut(s) 441, 723
NciI CCSGG 1 cut(s) 1366
NcoI CCATGG 1 cut(s) 639
NdeII GATC 8 cut(s) 13, 94, 106, 419, 766, 1419, 1552, 1772
NheI GCTAGC 1 cut(s) 970
NlaIV GGNNCC 1 cut(s) 421
NmuCI GTSAC 2 cut(s) 727, 898
NsiI ATGCAT 1 cut(s) 1571
NspI RCATGY 3 cut(s) 259, 316, 1569
NspV TTCGAA 1 cut(s) 505
PaeI GCATGC 1 cut(s) 1569
PciI ACATGT 1 cut(s) 255
PctI GAATGC 1 cut(s) 953
PdmI GAANNNNTTC 2 cut(s) 186, 1203
PfeI GAWTC 8 cut(s) 74, 182, 323, 365, 1204, 1301, 1529, 1742
PflMI CCANNNNNTGG 1 cut(s) 16
PkrI GCNGC 8 cut(s) 8, 618, 637, 844, 847, 1233, 1375, 1657
PleI GAGTC 3 cut(s) 30, 235, 895
PpsI GAGTC 3 cut(s) 30, 235, 895
PpuMI RGGWCCY 1 cut(s) 600
PscI ACATGT 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 600
Psp6I CCWGG 2 cut(s) 439, 721
PspGI CCWGG 2 cut(s) 439, 721
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 1 cut(s) 600
PspPPI RGGWCCY 1 cut(s) 600
PstNI CAGNNNCTG 2 cut(s) 556, 848
PsuI RGATCY 3 cut(s) 419, 1419, 1772
PvuII CAGCTG 1 cut(s) 848
RsaI GTAC 4 cut(s) 344, 454, 1409, 1580
RsaNI GTAC 4 cut(s) 343, 453, 1408, 1579
RseI CAYNNNNRTG 2 cut(s) 153, 1359
SatI GCNGC 8 cut(s) 7, 617, 636, 843, 846, 1232, 1374, 1656
Sau3AI GATC 8 cut(s) 13, 94, 106, 419, 766, 1419, 1552, 1772
Sau96I GGNCC 1 cut(s) 600
SchI GAGTC 3 cut(s) 30, 236, 895
ScrFI CCNGG 3 cut(s) 441, 723, 1366
SexAI ACCWGGT 1 cut(s) 721
SfaNI GCATC 3 cut(s) 553, 628, 957
SfcI CTRYAG 1 cut(s) 548
SfuI TTCGAA 1 cut(s) 505
SinI GGWCC 1 cut(s) 600
SmiMI CAYNNNNRTG 2 cut(s) 153, 1359
SmlI CTYRAG 6 cut(s) 40, 788, 935, 1055, 1665, 1796
SmoI CTYRAG 6 cut(s) 40, 788, 935, 1055, 1665, 1796
SphI GCATGC 1 cut(s) 1569
SsiI CCGC 2 cut(s) 6, 153
SspI AATATT 1 cut(s) 588
SspMI CTAG 7 cut(s) 128, 176, 297, 486, 944, 971, 1807
StyD4I CCNGG 3 cut(s) 439, 721, 1364
StyI CCWWGG 1 cut(s) 639
TaaI ACNGT 6 cut(s) 116, 142, 415, 552, 1496, 1793
TaiI ACGT 1 cut(s) 337
TaqI TCGA 6 cut(s) 34, 66, 368, 463, 505, 607
TauI GCSGC 1 cut(s) 9
TfiI GAWTC 8 cut(s) 74, 182, 323, 365, 1204, 1301, 1529, 1742
TscAI CASTG 6 cut(s) 202, 503, 753, 1004, 1274, 1712
TseFI GTSAC 2 cut(s) 727, 898
TseI GCWGC 7 cut(s) 616, 635, 842, 845, 1231, 1373, 1655
Tsp45I GTSAC 2 cut(s) 727, 898
TspDTI ATGAA 8 cut(s) 84, 288, 423, 534, 555, 1005, 1212, 1767
TspGWI ACGGA 3 cut(s) 175, 1294, 1638
TspRI CASTG 6 cut(s) 202, 503, 753, 1004, 1274, 1712
Van91I CCANNNNNTGG 1 cut(s) 16
Vha464I CTTAAG 2 cut(s) 40, 1055
VpaK11BI GGWCC 1 cut(s) 600
XagI CCTNNNNNAGG 1 cut(s) 675
XapI RAATTY 7 cut(s) 349, 521, 817, 1134, 1143, 1319, 1334
XbaI TCTAGA 1 cut(s) 943
XceI RCATGY 3 cut(s) 259, 316, 1569
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 799
XmnI GAANNNNTTC 2 cut(s) 186, 1203
XspI CTAG 7 cut(s) 128, 176, 297, 486, 944, 971, 1807
Zsp2I ATGCAT 1 cut(s) 1571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.