pycom12g19960

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005906
Physical Location & Seq
Reverse (-)
21638699 .. 21640334
1636 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1299 bp
ATGCCTACAGCTGAAGCTAAACCAGAACAACCGAAAGGAAGTGAAGAAGTTTCCCAACGCCCTCTCAACTACAAGATTTGGGTTTTGAAAGTCCCTATCCACTGTGAAGGCTGCAAGAAAAAGGTCGCAAAAATCTTGAAAAAAATCGATGGGGTTTACAAAACAGAGTTTGACGGGAACATCGGAAAGGAAAACAAAGTTACAGTGACTGGAAACGTGGAGCCGGAGATTTTGATCAGGAAACTGGCTAAGGGAGGGAAATATGCAGAGATGTGGCCTTGTCAGAAGGCTAACAACAGCAATAATTCAAATGACAAGAAGAAAAATAAGGAGAAACAGCAAGGCGAAGATACCCAAGGGTCCGAGGACGGCAACTATGGCGGAACTGGAGGCGGCGGTAATTGTGATGATGAAAGGGAGACGGTTGAGGCTGAAGTTGTTCAAGTTCAGGATAATAGTGGTAAGAAGAAAAATAAAGGTGGTAGCGGCGCGGGTGGGAGTAAGACCGCTGAGGGGGTTAATGCTGTGAAAGTGAATGAGGGGGGTGGTGTACCGGCGAAATCCGGCGGCGGTGGAAAGGGTAAAGAGGTGAAGGTTGAGGTGGTGAAGCAGGGGAAGCCTGTGAACATGTCTGAACAGCCGGCTGCGGTGAAGAAGTGTTTTGGTGGGGATGACGATGAAGATGACAGTAATTGTGAGGTTGATAAAAGTGGAGGCGGCGGCGGCGCTAGTAGCAGTGGAACTAAGAAGAACAAGAAGGGGCCAAGTGGGAAGGCCAATCCTGATGATTCAGATGAGGTTGAACAATGTGGTGATGCTGCTCCACGCACTGGATCACCACCAAATCATGGTAATGTACCACGTGGGCCTCCTATGCCTCAGGGGCCTTACCCTTACATGCTTCCATCTGGTACTCAAGGCCCTAATTTTGCTCCAGCTCTTGCTCCGGCTCCGGCTTCAGCACCAGCTCCAGCCAATCACATGGCTCCACATCAGCAGATCTATGAGTACCAGTACCCCAGGCAGAACTACAACGGGCTTCCCCTCCAAGCTATGAACTTCAATACGGCCTACCCCGCCAGGAGCTACGGTGCATCCCAGTATGCTGCCCCACTACCTCACGCACACTCTTACACTTATGCGTCTCAAAGTGTGGCAGCTGAGAGTGAGCTCAGGTCCTATCATTCGGACTCGTATCCGCAGTATTACCCGTCGTCGCCGGAGTATAATTCGCCGCAGCAATCCGATTCCTTCGTGCTGTTCAGCGACGAGAATCCGAACGGGTGCAGCATTATGTGA

Protein Analysis

433

Amino Acids

45.78

Weight (kDa)

6.43

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 29 - 84 2.7e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 830, 848
AccII CGCG 1 cut(s) 491
AclWI GGATC 1 cut(s) 841
AcuI CTGAAG 3 cut(s) 33, 453, 942
AcvI CACGTG 1 cut(s) 863
AfaI GTAC 5 cut(s) 552, 858, 913, 1010, 1016
AfiI CCNNNNNNNGG 3 cut(s) 513, 830, 848
AflIII ACRYGT 1 cut(s) 627
AgsI TTSAA 6 cut(s) 88, 139, 309, 443, 803, 1063
AjnI CCWGG 2 cut(s) 1019, 1079
AluBI AGCT 8 cut(s) 11, 17, 938, 968, 1052, 1086, 1160, 1171
AluI AGCT 8 cut(s) 11, 17, 938, 968, 1052, 1086, 1160, 1171
Alw21I GWGCWC 1 cut(s) 1173
Alw26I GTCTC 2 cut(s) 413, 1149
AlwI GGATC 1 cut(s) 841
AlwNI CAGNNNCTG 1 cut(s) 209
AoxI GGCC 7 cut(s) 275, 761, 774, 866, 884, 919, 1068
ApeKI GCWGC 7 cut(s) 111, 644, 818, 1106, 1157, 1237, 1287
Asp700I GAANNNNTTC 1 cut(s) 438
AspLEI GCGC 2 cut(s) 491, 728
AspS9I GGNCC 6 cut(s) 360, 761, 866, 884, 920, 1176
AsuHPI GGTGA 5 cut(s) 601, 616, 661, 824, 828
AvaII GGWCC 2 cut(s) 360, 1176
AxyI CCTNAGG 1 cut(s) 879
BanII GRGCYC 1 cut(s) 1173
BbrPI CACGTG 1 cut(s) 863
Bbv12I GWGCWC 1 cut(s) 1173
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 6 cut(s) 98, 631, 805, 1093, 1169, 1249
BccI CCATC 2 cut(s) 143, 913
BceAI ACGGC 2 cut(s) 385, 1083
BciT130I CCWGG 2 cut(s) 1021, 1081
BciVI GTATCC 1 cut(s) 1206
BclI TGATCA 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 413, 1149
BfaI CTAG 1 cut(s) 729
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 729
BfuI GTATCC 1 cut(s) 1206
BglI GCCNNNNNGGC 1 cut(s) 883
BglII AGATCT 1 cut(s) 999
Bme1390I CCNGG 2 cut(s) 1021, 1081
Bme18I GGWCC 2 cut(s) 360, 1176
BmgT120I GGNCC 6 cut(s) 360, 761, 866, 884, 920, 1176
BmiI GGNNCC 6 cut(s) 222, 361, 762, 885, 951, 987
BmrFI CCNGG 2 cut(s) 1021, 1081
BmrI ACTGGG 1 cut(s) 1093
BmsI GCATC 2 cut(s) 805, 1103
BmuI ACTGGG 1 cut(s) 1093
BpmI CTGGAG 3 cut(s) 408, 918, 954
Bpu10I CCTNAGC 3 cut(s) 249, 510, 1172
BpuEI CTTGAG 1 cut(s) 900
Bsa29I ATCGAT 1 cut(s) 147
BsaAI YACGTR 1 cut(s) 863
BsaBI GATNNNNATC 1 cut(s) 233
BsaJI CCNNGG 3 cut(s) 355, 363, 1019
BsaXI ACNNNNNCTCC 2 cut(s) 1075, 1105
Bsc4I CCNNNNNNNGG 3 cut(s) 513, 830, 848
Bse118I RCCGGY 2 cut(s) 553, 640
Bse1I ACTGG 6 cut(s) 214, 249, 391, 835, 1012, 1099
Bse21I CCTNAGG 1 cut(s) 879
Bse8I GATNNNNATC 1 cut(s) 233
BseBI CCWGG 2 cut(s) 1021, 1081
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 3 cut(s) 355, 363, 1019
BseGI GGATG 2 cut(s) 676, 1094
BseJI GATNNNNATC 1 cut(s) 233
BseLI CCNNNNNNNGG 3 cut(s) 513, 830, 848
BseMII CTCAG 4 cut(s) 501, 893, 1152, 1186
BseNI ACTGG 6 cut(s) 214, 249, 391, 835, 1012, 1099
BseXI GCAGC 6 cut(s) 98, 631, 805, 1093, 1169, 1249
Bsh1236I CGCG 1 cut(s) 491
BshFI GGCC 7 cut(s) 277, 763, 776, 868, 886, 921, 1070
BshVI ATCGAT 1 cut(s) 147
BsiHKAI GWGCWC 1 cut(s) 1173
BsiSI CCGG 7 cut(s) 224, 554, 564, 641, 947, 953, 1220
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 513, 830, 848
BsmAI GTCTC 2 cut(s) 413, 1149
BsmBI CGTCTC 2 cut(s) 413, 1149
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 7 cut(s) 277, 763, 776, 868, 886, 921, 1070
Bsp1286I GDGCHC 1 cut(s) 1173
Bsp143I GATC 3 cut(s) 234, 833, 999
BspANI GGCC 7 cut(s) 277, 763, 776, 868, 886, 921, 1070
BspCNI CTCAG 4 cut(s) 502, 892, 1153, 1185
BspDI ATCGAT 1 cut(s) 147
BspFNI CGCG 1 cut(s) 491
BspLI GGNNCC 6 cut(s) 222, 361, 762, 885, 951, 987
BspPI GGATC 1 cut(s) 841
BsrFI RCCGGY 2 cut(s) 553, 640
BsrI ACTGG 6 cut(s) 214, 249, 391, 835, 1012, 1099
BssAI RCCGGY 2 cut(s) 553, 640
BssECI CCNNGG 3 cut(s) 355, 363, 1019
BssMI GATC 3 cut(s) 234, 833, 999
BssT1I CCWWGG 1 cut(s) 355
Bst2UI CCWGG 2 cut(s) 1021, 1081
Bst4CI ACNGT 5 cut(s) 104, 205, 424, 689, 1091
BstBAI YACGTR 1 cut(s) 863
BstC8I GCNNGC 1 cut(s) 642
BstDEI CTNAG 6 cut(s) 249, 510, 744, 879, 1161, 1172
BstF5I GGATG 2 cut(s) 676, 1094
BstFNI CGCG 1 cut(s) 491
BstH2I RGCGCY 1 cut(s) 729
BstHHI GCGC 2 cut(s) 491, 728
BstKTI GATC 3 cut(s) 237, 836, 1002
BstMAI GTCTC 2 cut(s) 413, 1149
BstMBI GATC 3 cut(s) 234, 833, 999
BstMWI GCNNNNNNNGC 7 cut(s) 378, 616, 723, 732, 874, 883, 1076
BstNI CCWGG 2 cut(s) 1021, 1081
BstNSI RCATGY 2 cut(s) 631, 901
BstSCI CCNGG 2 cut(s) 1019, 1079
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 1 cut(s) 491
BstV1I GCAGC 6 cut(s) 98, 631, 805, 1093, 1169, 1249
BstX2I RGATCY 1 cut(s) 999
BstXI CCANNNNNNTGG 1 cut(s) 982
BstYI RGATCY 1 cut(s) 999
Bsu15I ATCGAT 1 cut(s) 147
Bsu36I CCTNAGG 1 cut(s) 879
BsuI GTATCC 1 cut(s) 1206
BsuRI GGCC 7 cut(s) 277, 763, 776, 868, 886, 921, 1070
BsuTUI ATCGAT 1 cut(s) 147
BtsCI GGATG 2 cut(s) 676, 1094
BtsI GCAGTG 1 cut(s) 742
BtsIMutI CAGTG 4 cut(s) 100, 210, 742, 828
Cac8I GCNNGC 1 cut(s) 642
CaiI CAGNNNCTG 1 cut(s) 209
CfoI GCGC 2 cut(s) 491, 728
Cfr10I RCCGGY 2 cut(s) 553, 640
Cfr13I GGNCC 6 cut(s) 360, 761, 866, 884, 920, 1176
ClaI ATCGAT 1 cut(s) 147
CseI GACGC 1 cut(s) 1131
Csp6I GTAC 5 cut(s) 551, 857, 912, 1009, 1015
CviAII CATG 4 cut(s) 628, 848, 898, 982
CviQI GTAC 5 cut(s) 551, 857, 912, 1009, 1015
DdeI CTNAG 6 cut(s) 249, 510, 744, 879, 1161, 1172
DpnI GATC 3 cut(s) 236, 835, 1001
DpnII GATC 3 cut(s) 234, 833, 999
EciI GGCGGA 1 cut(s) 396
Ecl136II GAGCTC 1 cut(s) 1171
Eco130I CCWWGG 1 cut(s) 355
Eco24I GRGCYC 1 cut(s) 1173
Eco47I GGWCC 2 cut(s) 360, 1176
Eco53kI GAGCTC 1 cut(s) 1171
Eco57I CTGAAG 3 cut(s) 33, 453, 942
Eco72I CACGTG 1 cut(s) 863
Eco81I CCTNAGG 1 cut(s) 879
EcoICRI GAGCTC 1 cut(s) 1171
EcoO109I RGGNCCY 3 cut(s) 884, 920, 1176
EcoRII CCWGG 2 cut(s) 1019, 1079
EcoT14I CCWWGG 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 1173
ErhI CCWWGG 1 cut(s) 355
Esp3I CGTCTC 2 cut(s) 413, 1149
FaeI CATG 4 cut(s) 631, 851, 901, 985
FaqI GGGAC 1 cut(s) 77
FatI CATG 4 cut(s) 627, 847, 897, 981
FauI CCCGC 2 cut(s) 484, 1084
FbaI TGATCA 1 cut(s) 234
FokI GGATG 2 cut(s) 683, 1081
FriOI GRGCYC 1 cut(s) 1173
FspBI CTAG 1 cut(s) 729
GlaI GCGC 2 cut(s) 490, 727
GsuI CTGGAG 3 cut(s) 408, 918, 954
HaeII RGCGCY 1 cut(s) 729
HaeIII GGCC 7 cut(s) 277, 763, 776, 868, 886, 921, 1070
HapII CCGG 7 cut(s) 224, 554, 564, 641, 947, 953, 1220
HgaI GACGC 1 cut(s) 1131
HhaI GCGC 2 cut(s) 491, 728
Hin1II CATG 4 cut(s) 631, 851, 901, 985
Hin6I GCGC 2 cut(s) 489, 726
HinP1I GCGC 2 cut(s) 489, 726
HinfI GANTC 4 cut(s) 788, 1190, 1247, 1273
HpaII CCGG 7 cut(s) 224, 554, 564, 641, 947, 953, 1220
HphI GGTGA 5 cut(s) 601, 616, 661, 824, 828
Hpy166II GTNNAC 3 cut(s) 157, 551, 625
Hpy188I TCNGA 8 cut(s) 185, 285, 364, 634, 793, 1189, 1246, 1278
Hpy188III TCNNGA 4 cut(s) 136, 238, 449, 782
Hpy8I GTNNAC 3 cut(s) 157, 551, 625
Hpy99I CGWCG 3 cut(s) 1216, 1219, 1271
HpyAV CCTTC 6 cut(s) 101, 280, 586, 751, 766, 1261
HpyCH4III ACNGT 5 cut(s) 104, 205, 424, 689, 1091
HpyCH4IV ACGT 2 cut(s) 216, 862
HpyCH4V TGCA 4 cut(s) 114, 266, 1094, 1287
HpyF10VI GCNNNNNNNGC 7 cut(s) 378, 616, 723, 732, 874, 883, 1076
HpyF3I CTNAG 6 cut(s) 249, 510, 744, 879, 1161, 1172
HpySE526I ACGT 2 cut(s) 216, 862
Hsp92II CATG 4 cut(s) 631, 851, 901, 985
HspAI GCGC 2 cut(s) 489, 726
KroI GCCGGC 1 cut(s) 640
KroNI GCCGGC 1 cut(s) 642
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 3 cut(s) 234, 833, 999
LmnI GCTCC 8 cut(s) 220, 826, 937, 949, 955, 973, 991, 1083
Lsp1109I GCAGC 6 cut(s) 98, 631, 805, 1093, 1169, 1249
LweI GCATC 2 cut(s) 805, 1103
MaeI CTAG 1 cut(s) 729
MaeII ACGT 2 cut(s) 216, 862
MaeIII GTNAC 2 cut(s) 199, 205
MalI GATC 3 cut(s) 236, 835, 1001
MboI GATC 3 cut(s) 234, 833, 999
MboII GAAGA 7 cut(s) 56, 331, 359, 478, 664, 692, 760
MflI RGATCY 1 cut(s) 999
MhlI GDGCHC 1 cut(s) 1173
MluCI AATT 5 cut(s) 304, 400, 691, 925, 1228
MlyI GAGTC 1 cut(s) 1184
MroNI GCCGGC 1 cut(s) 640
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 1 cut(s) 519
MslI CAYNNNNRTG 1 cut(s) 852
MspA1I CMGCKG 3 cut(s) 11, 509, 1160
MspI CCGG 7 cut(s) 224, 554, 564, 641, 947, 953, 1220
MspR9I CCNGG 2 cut(s) 1021, 1081
MvaI CCWGG 2 cut(s) 1021, 1081
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 7 cut(s) 378, 616, 723, 732, 874, 883, 1076
NaeI GCCGGC 1 cut(s) 642
NdeII GATC 3 cut(s) 234, 833, 999
NgoMIV GCCGGC 1 cut(s) 640
NlaIII CATG 4 cut(s) 631, 851, 901, 985
NlaIV GGNNCC 6 cut(s) 222, 361, 762, 885, 951, 987
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 2 cut(s) 631, 901
PciI ACATGT 1 cut(s) 627
PdiI GCCGGC 1 cut(s) 642
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 3 cut(s) 788, 1247, 1273
PflMI CCANNNNNTGG 2 cut(s) 830, 848
PleI GAGTC 1 cut(s) 1184
PmaCI CACGTG 1 cut(s) 863
PmlI CACGTG 1 cut(s) 863
PpsI GAGTC 1 cut(s) 1184
Ppu21I YACGTR 1 cut(s) 863
PpuMI RGGWCCY 1 cut(s) 1176
PscI ACATGT 1 cut(s) 627
Psp124BI GAGCTC 1 cut(s) 1173
Psp5II RGGWCCY 1 cut(s) 1176
Psp6I CCWGG 2 cut(s) 1019, 1079
PspCI CACGTG 1 cut(s) 863
PspGI CCWGG 2 cut(s) 1019, 1079
PspN4I GGNNCC 6 cut(s) 222, 361, 762, 885, 951, 987
PspPI GGNCC 6 cut(s) 360, 761, 866, 884, 920, 1176
PspPPI RGGWCCY 1 cut(s) 1176
PstNI CAGNNNCTG 1 cut(s) 209
PsuI RGATCY 1 cut(s) 999
PvuII CAGCTG 2 cut(s) 11, 1160
RsaI GTAC 5 cut(s) 552, 858, 913, 1010, 1016
RsaNI GTAC 5 cut(s) 551, 857, 912, 1009, 1015
RseI CAYNNNNRTG 1 cut(s) 852
SacI GAGCTC 1 cut(s) 1173
SaqAI TTAA 1 cut(s) 519
Sau3AI GATC 3 cut(s) 234, 833, 999
Sau96I GGNCC 6 cut(s) 360, 761, 866, 884, 920, 1176
SchI GAGTC 1 cut(s) 1184
ScrFI CCNGG 2 cut(s) 1021, 1081
SduI GDGCHC 1 cut(s) 1173
SfaNI GCATC 2 cut(s) 805, 1103
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 2 cut(s) 360, 1176
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 1 cut(s) 915
SmoI CTYRAG 1 cut(s) 915
Sse9I AATT 5 cut(s) 304, 400, 691, 925, 1228
SspMI CTAG 1 cut(s) 729
SstI GAGCTC 1 cut(s) 1173
StyD4I CCNGG 2 cut(s) 1019, 1079
StyI CCWWGG 1 cut(s) 355
TaaI ACNGT 5 cut(s) 104, 205, 424, 689, 1091
TaiI ACGT 2 cut(s) 219, 865
TaqI TCGA 1 cut(s) 147
TasI AATT 5 cut(s) 304, 400, 691, 925, 1228
TauI GCSGC 7 cut(s) 396, 489, 570, 720, 723, 726, 1237
TfiI GAWTC 3 cut(s) 788, 1247, 1273
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 4 cut(s) 107, 210, 742, 835
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 7 cut(s) 111, 644, 818, 1106, 1157, 1237, 1287
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 3 cut(s) 426, 693, 1070
TspRI CASTG 4 cut(s) 107, 210, 742, 835
Van91I CCANNNNNTGG 2 cut(s) 830, 848
VpaK11BI GGWCC 2 cut(s) 360, 1176
XceI RCATGY 2 cut(s) 631, 901
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.