pycom13g03450

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004080
Physical Location & Seq
Forward (+)
2283498 .. 2286080
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
ATGTTCCGGTTCCAGGGCCTGCGGGCATTTTCAATTGCTGACCCCACCCACGTGACCCTCCCCTCCTACTCGCGTGTCATCCAGACGTCACTCCACCTCCGCCATCAGTCAGTTTCCTTCCCGACGAAGTGGCCACGTCTCCGATCACTCCCCACCGTCAGGAGCTGCACTGCTCTCGCATTCGATTTCAAAGGTCTCGAAGGTTGCCTGCATTGGCGAGGCACTAGCATTGTCCGATTTGTGGCTCACATTCCCCGCACCTCTCACAGTATGCTTCAGCAATTACACAAATGTGATTTGCCTTGCCTGCAAAGAAAGAGAGTGAAAGACACAGAAAGAGAGAGGATGGAGGAGCAGGGAAAGCATGAGGAGAAGAAGAAGGTGATGGTGGCGATCGACGAAAGCGAGTTTAGCCTATACGCCTTCAATTGGGCGCTCGAAAATCTGCGCGACACCATCCAGAGTTCGCAGCTAGTCGTCTTCACCGCACAGCCCATTGACTTCGCTTCCACTTATGCTGCCAGTTACGGAGCTGCCCCTGCTGAGTTGGTAACATCTTTGCTTGAAAACCACAAGAAGGTGGCGCTAGCTTTGTTGGAGAGAGCTAAAGAAATTTGTGCCAAACATGGGATTGTTGCAGAGACAGTGACAGAGGTAGGCGATCCGAAGGTGGTGATATGCGAAGCAGTAGAGAAACACGACATCCAGTTGCTTGTGTTAGGCAGCCACGGTCGCGGAGGTATCCAAAGGGCTTTTTTGGGAAGCGTAAGCAATTACTGCGTACATAACGCTAAGTGTCCTGTTCTTGTTGTGAGGAAACGGGATTGA

Protein Analysis

276

Amino Acids

30.97

Weight (kDa)

9.21

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 126 - 272 2.6e-33 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 89
AccII CGCG 3 cut(s) 73, 450, 735
AciI CCGC 5 cut(s) 22, 100, 256, 486, 735
AclWI GGATC 1 cut(s) 656
AcoI YGGCCR 1 cut(s) 131
AcsI RAATTY 1 cut(s) 612
AcuI CTGAAG 1 cut(s) 260
AcvI CACGTG 1 cut(s) 52
AcyI GRCGYC 1 cut(s) 86
AfaI GTAC 1 cut(s) 783
AfiI CCNNNNNNNGG 6 cut(s) 13, 159, 241, 429, 577, 627
AgsI TTSAA 4 cut(s) 33, 190, 427, 566
AjiI CACGTC 1 cut(s) 137
AjnI CCWGG 1 cut(s) 12
AleI CACNNNNGTG 2 cut(s) 50, 291
AluBI AGCT 5 cut(s) 165, 472, 533, 590, 605
AluI AGCT 5 cut(s) 165, 472, 533, 590, 605
Alw26I GTCTC 3 cut(s) 143, 200, 635
AlwI GGATC 1 cut(s) 656
AlwNI CAGNNNCTG 2 cut(s) 19, 165
AoxI GGCC 2 cut(s) 16, 131
ApeKI GCWGC 5 cut(s) 165, 469, 518, 533, 723
ApoI RAATTY 1 cut(s) 612
AspLEI GCGC 3 cut(s) 436, 450, 586
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 3 cut(s) 394, 475, 685
AsuNHI GCTAGC 1 cut(s) 586
BalI TGGCCA 1 cut(s) 133
BbrPI CACGTG 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 472
BbvI GCAGC 5 cut(s) 152, 481, 505, 520, 735
BccI CCATC 4 cut(s) 111, 340, 379, 464
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 1 cut(s) 14
BciVI GTATCC 1 cut(s) 752
BcoDI GTCTC 3 cut(s) 143, 200, 635
BfaI CTAG 3 cut(s) 225, 473, 587
BfoI RGCGCY 2 cut(s) 437, 587
BfuI GTATCC 1 cut(s) 752
BisI GCNGC 5 cut(s) 166, 470, 519, 534, 724
BlsI GCNGC 5 cut(s) 167, 471, 520, 535, 725
Bme1390I CCNGG 1 cut(s) 14
BmgBI CACGTC 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 1 cut(s) 11
BmrFI CCNGG 1 cut(s) 14
BmtI GCTAGC 1 cut(s) 590
BpiI GAAGAC 1 cut(s) 472
BsaAI YACGTR 1 cut(s) 52
BsaHI GRCGYC 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 2 cut(s) 13, 727
BsaWI WCCGGW 1 cut(s) 6
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 6 cut(s) 13, 159, 241, 429, 577, 627
Bse1I ACTGG 2 cut(s) 522, 706
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 13, 727
BseGI GGATG 4 cut(s) 78, 351, 456, 702
BseLI CCNNNNNNNGG 6 cut(s) 13, 159, 241, 429, 577, 627
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 522, 706
BseRI GAGGAG 2 cut(s) 365, 383
BseXI GCAGC 5 cut(s) 152, 481, 505, 520, 735
BsgI GTGCAG 1 cut(s) 151
Bsh1236I CGCG 3 cut(s) 73, 450, 735
Bsh1285I CGRYCG 2 cut(s) 396, 733
BshFI GGCC 2 cut(s) 18, 133
BsiEI CGRYCG 2 cut(s) 396, 733
BsiSI CCGG 1 cut(s) 7
BslI CCNNNNNNNGG 6 cut(s) 13, 159, 241, 429, 577, 627
BsmAI GTCTC 3 cut(s) 143, 200, 635
BsmBI CGTCTC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 2 cut(s) 18, 133
Bso31I GGTCTC 1 cut(s) 200
Bsp143I GATC 3 cut(s) 143, 393, 661
BspACI CCGC 5 cut(s) 22, 100, 256, 486, 735
BspANI GGCC 2 cut(s) 18, 133
BspCNI CTCAG 1 cut(s) 535
BspFNI CGCG 3 cut(s) 73, 450, 735
BspLI GGNNCC 1 cut(s) 11
BspOI GCTAGC 1 cut(s) 590
BspPI GGATC 1 cut(s) 656
BspTNI GGTCTC 1 cut(s) 200
BsrI ACTGG 2 cut(s) 522, 706
BssECI CCNNGG 2 cut(s) 13, 727
BssMI GATC 3 cut(s) 143, 393, 661
BssNI GRCGYC 1 cut(s) 86
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 4 cut(s) 157, 269, 646, 731
BstACI GRCGYC 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 777
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 5 cut(s) 20, 24, 209, 308, 588
BstDEI CTNAG 2 cut(s) 543, 792
BstDSI CCRYGG 1 cut(s) 727
BstF5I GGATG 4 cut(s) 78, 351, 456, 702
BstFNI CGCG 3 cut(s) 73, 450, 735
BstH2I RGCGCY 2 cut(s) 437, 587
BstHHI GCGC 3 cut(s) 436, 450, 586
BstKTI GATC 3 cut(s) 146, 396, 664
BstMAI GTCTC 3 cut(s) 143, 200, 635
BstMBI GATC 3 cut(s) 143, 393, 661
BstMCI CGRYCG 2 cut(s) 396, 733
BstMWI GCNNNNNNNGC 6 cut(s) 307, 361, 411, 539, 732, 777
BstNI CCWGG 1 cut(s) 14
BstSCI CCNGG 1 cut(s) 12
BstUI CGCG 3 cut(s) 73, 450, 735
BstV1I GCAGC 5 cut(s) 152, 481, 505, 520, 735
BstV2I GAAGAC 1 cut(s) 472
BsuI GTATCC 1 cut(s) 752
BsuRI GGCC 2 cut(s) 18, 133
BtgI CCRYGG 1 cut(s) 727
BtrI CACGTC 1 cut(s) 137
BtsCI GGATG 4 cut(s) 78, 351, 456, 702
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 2 cut(s) 168, 651
Cac8I GCNNGC 5 cut(s) 20, 24, 209, 308, 588
CaiI CAGNNNCTG 2 cut(s) 19, 165
CfoI GCGC 3 cut(s) 436, 450, 586
Cfr13I GGNCC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 782
CviAII CATG 2 cut(s) 365, 626
CviQI GTAC 1 cut(s) 782
DdeI CTNAG 2 cut(s) 543, 792
DpnI GATC 3 cut(s) 145, 395, 663
DpnII GATC 3 cut(s) 143, 393, 661
EaeI YGGCCR 1 cut(s) 131
EciI GGCGGA 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 260
Eco72I CACGTG 1 cut(s) 52
EcoO109I RGGNCCY 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 12
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 2 cut(s) 368, 629
FaiI YATR 7 cut(s) 272, 366, 418, 516, 627, 679, 786
FatI CATG 2 cut(s) 364, 625
FauI CCCGC 2 cut(s) 15, 263
Fnu4HI GCNGC 5 cut(s) 166, 470, 519, 534, 724
FokI GGATG 4 cut(s) 65, 358, 443, 689
Fsp4HI GCNGC 5 cut(s) 166, 470, 519, 534, 724
FspBI CTAG 3 cut(s) 225, 473, 587
GlaI GCGC 3 cut(s) 435, 449, 585
GluI GCNGC 5 cut(s) 166, 470, 519, 534, 724
HaeII RGCGCY 2 cut(s) 437, 587
HaeIII GGCC 2 cut(s) 18, 133
HapII CCGG 1 cut(s) 7
HhaI GCGC 3 cut(s) 436, 450, 586
Hin1I GRCGYC 1 cut(s) 86
Hin1II CATG 2 cut(s) 368, 629
Hin6I GCGC 3 cut(s) 434, 448, 584
HinP1I GCGC 3 cut(s) 434, 448, 584
HpaII CCGG 1 cut(s) 7
HphI GGTGA 3 cut(s) 394, 475, 685
Hpy188I TCNGA 3 cut(s) 143, 236, 666
Hpy188III TCNNGA 5 cut(s) 82, 121, 160, 197, 460
Hpy99I CGWCG 2 cut(s) 127, 401
HpyAV CCTTC 6 cut(s) 127, 194, 373, 433, 571, 661
HpyCH4III ACNGT 4 cut(s) 157, 269, 646, 731
HpyCH4IV ACGT 3 cut(s) 51, 86, 136
HpyCH4V TGCA 4 cut(s) 168, 211, 310, 638
HpyF10VI GCNNNNNNNGC 6 cut(s) 307, 361, 411, 539, 732, 777
HpyF3I CTNAG 2 cut(s) 543, 792
HpySE526I ACGT 3 cut(s) 51, 86, 136
Hsp92I GRCGYC 1 cut(s) 86
Hsp92II CATG 2 cut(s) 368, 629
HspAI GCGC 3 cut(s) 434, 448, 584
Kzo9I GATC 3 cut(s) 143, 393, 661
LmnI GCTCC 3 cut(s) 162, 352, 530
Lsp1109I GCAGC 5 cut(s) 152, 481, 505, 520, 735
MaeI CTAG 3 cut(s) 225, 473, 587
MaeII ACGT 3 cut(s) 51, 86, 136
MaeIII GTNAC 5 cut(s) 52, 87, 524, 550, 646
MalI GATC 3 cut(s) 145, 395, 663
MboI GATC 3 cut(s) 143, 393, 661
MboII GAAGA 3 cut(s) 385, 388, 472
MfeI CAATTG 2 cut(s) 33, 427
MlsI TGGCCA 1 cut(s) 133
MluCI AATT 5 cut(s) 33, 281, 427, 612, 772
MluNI TGGCCA 1 cut(s) 133
MmeI TCCRAC 1 cut(s) 576
Mox20I TGGCCA 1 cut(s) 133
MscI TGGCCA 1 cut(s) 133
MslI CAYNNNNRTG 2 cut(s) 50, 291
Msp20I TGGCCA 1 cut(s) 133
MspI CCGG 1 cut(s) 7
MspR9I CCNGG 1 cut(s) 14
MunI CAATTG 2 cut(s) 33, 427
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 1 cut(s) 14
MvnI CGCG 3 cut(s) 73, 450, 735
MwoI GCNNNNNNNGC 6 cut(s) 307, 361, 411, 539, 732, 777
NdeII GATC 3 cut(s) 143, 393, 661
NheI GCTAGC 1 cut(s) 586
NlaIII CATG 2 cut(s) 368, 629
NlaIV GGNNCC 1 cut(s) 11
NmuCI GTSAC 3 cut(s) 52, 87, 646
OliI CACNNNNGTG 2 cut(s) 50, 291
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PctI GAATGC 1 cut(s) 179
PkrI GCNGC 5 cut(s) 167, 471, 520, 535, 725
Ple19I CGATCG 1 cut(s) 396
PmaCI CACGTG 1 cut(s) 52
PmlI CACGTG 1 cut(s) 52
Ppu21I YACGTR 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 12
PspCI CACGTG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 11
PspPI GGNCC 1 cut(s) 16
PstNI CAGNNNCTG 2 cut(s) 19, 165
PvuI CGATCG 1 cut(s) 396
RsaI GTAC 1 cut(s) 783
RsaNI GTAC 1 cut(s) 782
RseI CAYNNNNRTG 2 cut(s) 50, 291
SatI GCNGC 5 cut(s) 166, 470, 519, 534, 724
Sau3AI GATC 3 cut(s) 143, 393, 661
Sau96I GGNCC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 14
SmiMI CAYNNNNRTG 2 cut(s) 50, 291
Sse9I AATT 5 cut(s) 33, 281, 427, 612, 772
SsiI CCGC 5 cut(s) 22, 100, 256, 486, 735
SspMI CTAG 3 cut(s) 225, 473, 587
StyD4I CCNGG 1 cut(s) 12
TaaI ACNGT 4 cut(s) 157, 269, 646, 731
TaiI ACGT 3 cut(s) 54, 89, 139
TaqI TCGA 4 cut(s) 183, 198, 396, 438
TasI AATT 5 cut(s) 33, 281, 427, 612, 772
TscAI CASTG 2 cut(s) 175, 651
TseFI GTSAC 3 cut(s) 52, 87, 646
TseI GCWGC 5 cut(s) 165, 469, 518, 533, 723
Tsp45I GTSAC 3 cut(s) 52, 87, 646
TspGWI ACGGA 1 cut(s) 543
TspRI CASTG 2 cut(s) 175, 651
XapI RAATTY 1 cut(s) 612
XspI CTAG 3 cut(s) 225, 473, 587
ZraI GACGTC 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.