Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

17033494 .. 17037789

Gene Structure 4296 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1083 bp

ATGGCGCAACAGCAGCTAACCAAGTGCAGCACCTGGCCCACCGCTCCCAATCTCCGTCTTCCCCGCCAAAACCCCCAGCGCTTTGCCTTTCCCAATGCCGGGCACCGGAATCCCAATCAGAAGAGTTTGAATTGGCTGATGCGAGGGTTTGGTGGTGATGCTCCGGCGGTGGCGGTGAAGGCGAGTAATGGCGGGGCAGGGACTGAGCTGTCGGAGACTGCGGTGGTGGTCGACAGGCCGAAACTGCATCGTTTTCAGGTGTCCGATGGGTATCCGAGTCCGTTCGGTGCCACGGCGCGTGATGGCGGGGTTAACTTCGCCGTCTATTCCGGCAATGCGGTTTCCGTCACTCTCTGTTTGATCGCTCTCTCCGATTTAGAGGAGGATAAAGTTTCTGAGCAGATTCCGCTTGATTCTTCGACCAACAAGTCCGGAAATGTGTGGCATGTACTTCTCAAGGGAGATTTCAAAGATACGCTTTATGGTTACAAATTTGATGGGAAGTTTTCGCCCGAAGAGGGACACTACTATGACTCTTCTAGAGTTGTATTGGATCCTTATGCAAAGGCAGTTATCCGCAGAGGAGAATTCCGCAAGCTAGGTCCTGATGGTAACTGCTGGCCTCAAATGGCTGGAACGGTACCTTCTTTTGATGATCAGTTTGACTGGGAAGGAGATTTACCACTGAAGTATCCACAAAAAGACCTTATCATATATGAAATGCATGTACGTGGGTTCACAAGGCATGAATCAAGTGCGACTGAGTTTCCCGGTACATACCTTGGTTTGGTGGACAAGCTTGATCATTTAAAGGAGCTCGGGATCAACTGTTTAGAGTTAATGCCATGTCATGAGTTCAATGAGTTGGAGTACTTCAGCTACAATTCTGTCTTGGGTGACTATAAGGTAAATTTTTGGGGATACTCGACTCTCAATTACTTTTCACCAATGATAAGATATTCATCTGCTGGCATACGTAATTGTGGCCGTGATGCAATAAATGAAGTCAAGTTTCTTATTAGAGAAGCACATAAACGTGGAATTGAGGTCGATCATGGACGTCGTCTTCAATCACACAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

361

Amino Acids

40.45

Weight (kDa)

6.42

Isoelectric Point (pI)

39.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	94 - 188	2.1e-15	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	208, 427
AatII	GACGTC	1 cut(s)	1063
Acc65I	GGTACC	1 cut(s)	640
AccB1I	GGYRCC	3 cut(s)	102, 287, 640
AccBSI	CCGCTC	1 cut(s)	44
AccI	GTMKAC	1 cut(s)	231
AccII	CGCG	1 cut(s)	298
AccIII	TCCGGA	1 cut(s)	431
AclWI	GGATC	3 cut(s)	548, 561, 830
AcoI	YGGCCR	1 cut(s)	985
AcsI	RAATTY	3 cut(s)	491, 587, 910
AcuI	CTGAAG	2 cut(s)	707, 859
AcyI	GRCGYC	1 cut(s)	1060
AfaI	GTAC	5 cut(s)	450, 642, 729, 775, 872
AfeI	AGCGCT	1 cut(s)	80
AfiI	CCNNNNNNNGG	5 cut(s)	98, 99, 105, 518, 787
AgsI	TTSAA	4 cut(s)	130, 469, 859, 1070
AjnI	CCWGG	1 cut(s)	32
AluBI	AGCT	7 cut(s)	16, 208, 598, 799, 817, 879, 1080
AluI	AGCT	7 cut(s)	16, 208, 598, 799, 817, 879, 1080
Alw21I	GWGCWC	1 cut(s)	819
Alw26I	GTCTC	1 cut(s)	209
AlwI	GGATC	3 cut(s)	548, 561, 830
AlwNI	CAGNNNCTG	2 cut(s)	33, 203
Ama87I	CYCGRG	1 cut(s)	818
Aor13HI	TCCGGA	1 cut(s)	431
Aor51HI	AGCGCT	1 cut(s)	80
AoxI	GGCC	4 cut(s)	35, 236, 620, 985
ApeKI	GCWGC	2 cut(s)	13, 27
ApoI	RAATTY	3 cut(s)	491, 587, 910
Asp718I	GGTACC	1 cut(s)	640
AspLEI	GCGC	3 cut(s)	7, 81, 298
AspS9I	GGNCC	2 cut(s)	36, 602
AsuC2I	CCSGG	2 cut(s)	100, 771
AsuHPI	GGTGA	4 cut(s)	167, 187, 908, 936
AvaI	CYCGRG	1 cut(s)	818
AvaII	GGWCC	1 cut(s)	602
BaeGI	GKGCMC	1 cut(s)	105
BaeI	ACNNNNGTAYC	2 cut(s)	674, 707
BamHI	GGATCC	1 cut(s)	553
BanI	GGYRCC	3 cut(s)	102, 287, 640
BanII	GRGCYC	1 cut(s)	819
BarI	GAAGNNNNNNTAC	2 cut(s)	663, 695
BbsI	GAAGAC	2 cut(s)	50, 1058
Bbv12I	GWGCWC	1 cut(s)	819
BbvI	GCAGC	2 cut(s)	25, 39
BccI	CCATC	4 cut(s)	260, 296, 491, 602
BceAI	ACGGC	3 cut(s)	305, 309, 972
BciT130I	CCWGG	1 cut(s)	34
BciVI	GTATCC	3 cut(s)	282, 702, 914
BclI	TGATCA	2 cut(s)	655, 802
BcnI	CCSGG	2 cut(s)	100, 771
BcoDI	GTCTC	1 cut(s)	209
BfaI	CTAG	2 cut(s)	540, 599
BfoI	RGCGCY	1 cut(s)	82
BfuI	GTATCC	3 cut(s)	282, 702, 914
BisI	GCNGC	2 cut(s)	14, 28
BlsI	GCNGC	2 cut(s)	15, 29
BmcAI	AGTACT	1 cut(s)	872
Bme1390I	CCNGG	3 cut(s)	34, 100, 771
Bme18I	GGWCC	1 cut(s)	602
BmeT110I	CYCGRG	1 cut(s)	818
BmgT120I	GGNCC	2 cut(s)	36, 602
BmiI	GGNNCC	4 cut(s)	104, 289, 555, 642
BmrFI	CCNGG	3 cut(s)	34, 100, 771
BmrI	ACTGGG	1 cut(s)	676
BmsI	GCATC	4 cut(s)	129, 148, 256, 982
BmuI	ACTGGG	1 cut(s)	676
BpiI	GAAGAC	2 cut(s)	50, 1058
BpuEI	CTTGAG	1 cut(s)	440
BpuMI	CCSGG	2 cut(s)	100, 771
BsaAI	YACGTR	2 cut(s)	731, 977
BsaBI	GATNNNNATC	2 cut(s)	270, 961
BsaHI	GRCGYC	1 cut(s)	1060
BsaJI	CCNNGG	2 cut(s)	291, 781
BsaWI	WCCGGW	2 cut(s)	105, 431
Bsc4I	CCNNNNNNNGG	5 cut(s)	98, 99, 105, 518, 787
Bse1I	ACTGG	1 cut(s)	671
Bse3DI	GCAATG	1 cut(s)	340
Bse8I	GATNNNNATC	2 cut(s)	270, 961
BseAI	TCCGGA	1 cut(s)	431
BseBI	CCWGG	1 cut(s)	34
BseDI	CCNNGG	2 cut(s)	291, 781
BseJI	GATNNNNATC	2 cut(s)	270, 961
BseLI	CCNNNNNNNGG	5 cut(s)	98, 99, 105, 518, 787
BseMI	GCAATG	1 cut(s)	340
BseMII	CTCAG	3 cut(s)	195, 387, 753
BseNI	ACTGG	1 cut(s)	671
BseRI	GAGGAG	2 cut(s)	395, 597
BseSI	GKGCMC	1 cut(s)	105
BseXI	GCAGC	2 cut(s)	25, 39
BseYI	CCCAGC	1 cut(s)	75
BsgI	GTGCAG	1 cut(s)	46
Bsh1236I	CGCG	1 cut(s)	298
BshFI	GGCC	4 cut(s)	37, 238, 622, 987
BshNI	GGYRCC	3 cut(s)	102, 287, 640
BsiHKAI	GWGCWC	1 cut(s)	819
BsiHKCI	CYCGRG	1 cut(s)	818
BsiSI	CCGG	6 cut(s)	99, 106, 164, 330, 432, 771
BslFI	GGGAC	2 cut(s)	214, 534
BslI	CCNNNNNNNGG	5 cut(s)	98, 99, 105, 518, 787
BsmAI	GTCTC	1 cut(s)	209
BsmFI	GGGAC	2 cut(s)	214, 534
BsnI	GGCC	4 cut(s)	37, 238, 622, 987
BsoBI	CYCGRG	1 cut(s)	818
Bsp1286I	GDGCHC	2 cut(s)	105, 819
Bsp13I	TCCGGA	1 cut(s)	431
Bsp143I	GATC	6 cut(s)	360, 553, 655, 802, 822, 1051
BspANI	GGCC	4 cut(s)	37, 238, 622, 987
BspCNI	CTCAG	3 cut(s)	196, 388, 754
BspEI	TCCGGA	1 cut(s)	431
BspFNI	CGCG	1 cut(s)	298
BspHI	TCATGA	1 cut(s)	850
BspLI	GGNNCC	4 cut(s)	104, 289, 555, 642
BspPI	GGATC	3 cut(s)	548, 561, 830
BspT107I	GGYRCC	3 cut(s)	102, 287, 640
BsrBI	CCGCTC	1 cut(s)	44
BsrDI	GCAATG	1 cut(s)	340
BsrI	ACTGG	1 cut(s)	671
BssECI	CCNNGG	2 cut(s)	291, 781
BssMI	GATC	6 cut(s)	360, 553, 655, 802, 822, 1051
BssNI	GRCGYC	1 cut(s)	1060
BssT1I	CCWWGG	1 cut(s)	781
Bst2UI	CCWGG	1 cut(s)	34
Bst4CI	ACNGT	2 cut(s)	640, 830
Bst6I	CTCTTC	3 cut(s)	116, 510, 541
BstACI	GRCGYC	1 cut(s)	1060
BstBAI	YACGTR	2 cut(s)	731, 977
BstC8I	GCNNGC	3 cut(s)	596, 620, 970
BstDEI	CTNAG	3 cut(s)	204, 396, 762
BstDSI	CCRYGG	1 cut(s)	291
BstFNI	CGCG	1 cut(s)	298
BstH2I	RGCGCY	1 cut(s)	82
BstHHI	GCGC	3 cut(s)	7, 81, 298
BstKTI	GATC	6 cut(s)	363, 556, 658, 805, 825, 1054
BstMAI	GTCTC	1 cut(s)	209
BstMBI	GATC	6 cut(s)	360, 553, 655, 802, 822, 1051
BstMWI	GCNNNNNNNGC	4 cut(s)	13, 179, 244, 406
BstNI	CCWGG	1 cut(s)	34
BstNSI	RCATGY	2 cut(s)	449, 728
BstSCI	CCNGG	3 cut(s)	32, 98, 769
BstSLI	GKGCMC	1 cut(s)	105
BstSNI	TACGTA	1 cut(s)	977
BstUI	CGCG	1 cut(s)	298
BstV1I	GCAGC	2 cut(s)	25, 39
BstV2I	GAAGAC	2 cut(s)	50, 1058
BstX2I	RGATCY	1 cut(s)	553
BstYI	RGATCY	1 cut(s)	553
BsuI	GTATCC	3 cut(s)	282, 702, 914
BsuRI	GGCC	4 cut(s)	37, 238, 622, 987
BtgI	CCRYGG	1 cut(s)	291
BtsIMutI	CAGTG	1 cut(s)	683
Cac8I	GCNNGC	3 cut(s)	596, 620, 970
CaiI	CAGNNNCTG	2 cut(s)	33, 203
CciI	TCATGA	1 cut(s)	850
CfoI	GCGC	3 cut(s)	7, 81, 298
Cfr13I	GGNCC	2 cut(s)	36, 602
Csp6I	GTAC	5 cut(s)	449, 641, 728, 774, 871
CviAII	CATG	6 cut(s)	446, 725, 746, 846, 851, 1055
CviQI	GTAC	5 cut(s)	449, 641, 728, 774, 871
DdeI	CTNAG	3 cut(s)	204, 396, 762
DpnI	GATC	6 cut(s)	362, 555, 657, 804, 824, 1053
DpnII	GATC	6 cut(s)	360, 553, 655, 802, 822, 1051
DraI	TTTAAA	1 cut(s)	810
DrdI	GACNNNNNNGTC	2 cut(s)	208, 427
DseDI	GACNNNNNNGTC	2 cut(s)	208, 427
EaeI	YGGCCR	1 cut(s)	985
Eam1104I	CTCTTC	3 cut(s)	116, 510, 541
EarI	CTCTTC	3 cut(s)	116, 510, 541
Ecl136II	GAGCTC	1 cut(s)	817
Eco105I	TACGTA	1 cut(s)	977
Eco130I	CCWWGG	1 cut(s)	781
Eco24I	GRGCYC	1 cut(s)	819
Eco47I	GGWCC	1 cut(s)	602
Eco47III	AGCGCT	1 cut(s)	80
Eco53kI	GAGCTC	1 cut(s)	817
Eco57I	CTGAAG	2 cut(s)	707, 859
Eco88I	CYCGRG	1 cut(s)	818
EcoICRI	GAGCTC	1 cut(s)	817
EcoO109I	RGGNCCY	1 cut(s)	602
EcoRI	GAATTC	1 cut(s)	587
EcoRII	CCWGG	1 cut(s)	32
EcoT14I	CCWWGG	1 cut(s)	781
EcoT22I	ATGCAT	1 cut(s)	726
EcoT38I	GRGCYC	1 cut(s)	819
ErhI	CCWWGG	1 cut(s)	781
FaeI	CATG	6 cut(s)	449, 728, 749, 849, 854, 1058
FalI	AAGNNNNNCTT	2 cut(s)	462, 494
FaqI	GGGAC	2 cut(s)	214, 534
FatI	CATG	6 cut(s)	445, 724, 745, 845, 850, 1054
FauI	CCCGC	3 cut(s)	71, 185, 299
FbaI	TGATCA	2 cut(s)	655, 802
FblI	GTMKAC	1 cut(s)	231
Fnu4HI	GCNGC	2 cut(s)	14, 28
FriOI	GRGCYC	1 cut(s)	819
Fsp4HI	GCNGC	2 cut(s)	14, 28
FspBI	CTAG	2 cut(s)	540, 599
GlaI	GCGC	3 cut(s)	6, 80, 297
GluI	GCNGC	2 cut(s)	14, 28
GsaI	CCCAGC	1 cut(s)	79
HaeII	RGCGCY	1 cut(s)	82
HaeIII	GGCC	4 cut(s)	37, 238, 622, 987
HapII	CCGG	6 cut(s)	99, 106, 164, 330, 432, 771
HhaI	GCGC	3 cut(s)	7, 81, 298
Hin1I	GRCGYC	1 cut(s)	1060
Hin1II	CATG	6 cut(s)	449, 728, 749, 849, 854, 1058
Hin6I	GCGC	3 cut(s)	5, 79, 296
HinP1I	GCGC	3 cut(s)	5, 79, 296
HincII	GTYRAC	2 cut(s)	232, 313
HindII	GTYRAC	2 cut(s)	232, 313
HindIII	AAGCTT	1 cut(s)	797
HinfI	GANTC	7 cut(s)	109, 277, 403, 413, 533, 749, 928
HpaI	GTTAAC	1 cut(s)	313
HpaII	CCGG	6 cut(s)	99, 106, 164, 330, 432, 771
HphI	GGTGA	4 cut(s)	167, 187, 908, 936
Hpy166II	GTNNAC	4 cut(s)	232, 313, 738, 793
Hpy188I	TCNGA	6 cut(s)	120, 214, 265, 276, 373, 397
Hpy188III	TCNNGA	5 cut(s)	432, 540, 605, 820, 851
Hpy8I	GTNNAC	4 cut(s)	232, 313, 738, 793
Hpy99I	CGWCG	1 cut(s)	1065
HpyAV	CCTTC	3 cut(s)	172, 654, 665
HpyCH4III	ACNGT	2 cut(s)	640, 830
HpyCH4IV	ACGT	4 cut(s)	730, 976, 1036, 1060
HpyCH4V	TGCA	5 cut(s)	27, 247, 563, 724, 995
HpyF10VI	GCNNNNNNNGC	4 cut(s)	13, 179, 244, 406
HpyF3I	CTNAG	3 cut(s)	204, 396, 762
HpySE526I	ACGT	4 cut(s)	730, 976, 1036, 1060
Hsp92I	GRCGYC	1 cut(s)	1060
Hsp92II	CATG	6 cut(s)	449, 728, 749, 849, 854, 1058
HspAI	GCGC	3 cut(s)	5, 79, 296
Kpn2I	TCCGGA	1 cut(s)	431
KpnI	GGTACC	1 cut(s)	644
Ksp22I	TGATCA	2 cut(s)	655, 802
KspAI	GTTAAC	1 cut(s)	313
Kzo9I	GATC	6 cut(s)	360, 553, 655, 802, 822, 1051
LmnI	GCTCC	3 cut(s)	49, 166, 814
Lsp1109I	GCAGC	2 cut(s)	25, 39
LweI	GCATC	4 cut(s)	129, 148, 256, 982
MaeI	CTAG	2 cut(s)	540, 599
MaeII	ACGT	4 cut(s)	730, 976, 1036, 1060
MaeIII	GTNAC	4 cut(s)	346, 485, 611, 896
MalI	GATC	6 cut(s)	362, 555, 657, 804, 824, 1053
MbiI	CCGCTC	1 cut(s)	44
MboI	GATC	6 cut(s)	360, 553, 655, 802, 822, 1051
MboII	GAAGA	6 cut(s)	50, 133, 408, 527, 528, 1058
MflI	RGATCY	1 cut(s)	553
MhlI	GDGCHC	2 cut(s)	105, 819
MluCI	AATT	8 cut(s)	130, 491, 587, 883, 910, 934, 979, 1041
MlyI	GAGTC	3 cut(s)	286, 527, 922
MmeI	TCCRAC	2 cut(s)	192, 846
MnlI	CCTC	7 cut(s)	137, 373, 376, 511, 575, 633, 1039
Mph1103I	ATGCAT	1 cut(s)	726
MroI	TCCGGA	1 cut(s)	431
MseI	TTAA	3 cut(s)	312, 809, 839
MslI	CAYNNNNRTG	3 cut(s)	528, 729, 1035
MspA1I	CMGCKG	1 cut(s)	1080
MspI	CCGG	6 cut(s)	99, 106, 164, 330, 432, 771
MspR9I	CCNGG	3 cut(s)	34, 100, 771
MvaI	CCWGG	1 cut(s)	34
MvnI	CGCG	1 cut(s)	298
MwoI	GCNNNNNNNGC	4 cut(s)	13, 179, 244, 406
NciI	CCSGG	2 cut(s)	100, 771
NdeII	GATC	6 cut(s)	360, 553, 655, 802, 822, 1051
NlaIII	CATG	6 cut(s)	449, 728, 749, 849, 854, 1058
NlaIV	GGNNCC	4 cut(s)	104, 289, 555, 642
NmuCI	GTSAC	2 cut(s)	346, 896
NsiI	ATGCAT	1 cut(s)	726
NspI	RCATGY	2 cut(s)	449, 728
PagI	TCATGA	1 cut(s)	850
PcsI	WCGNNNNNNNCGW	1 cut(s)	369
PfeI	GAWTC	4 cut(s)	109, 403, 413, 749
PflFI	GACNNNGTC	1 cut(s)	1062
PkrI	GCNGC	2 cut(s)	15, 29
PleI	GAGTC	3 cut(s)	285, 527, 922
PpsI	GAGTC	3 cut(s)	285, 527, 922
Ppu21I	YACGTR	2 cut(s)	731, 977
PpuMI	RGGWCCY	1 cut(s)	602
Psp124BI	GAGCTC	1 cut(s)	819
Psp5II	RGGWCCY	1 cut(s)	602
Psp6I	CCWGG	1 cut(s)	32
PspFI	CCCAGC	1 cut(s)	75
PspGI	CCWGG	1 cut(s)	32
PspN4I	GGNNCC	4 cut(s)	104, 289, 555, 642
PspPI	GGNCC	2 cut(s)	36, 602
PspPPI	RGGWCCY	1 cut(s)	602
PstNI	CAGNNNCTG	2 cut(s)	33, 203
PsuI	RGATCY	1 cut(s)	553
PsyI	GACNNNGTC	1 cut(s)	1062
PvuII	CAGCTG	1 cut(s)	1080
RsaI	GTAC	5 cut(s)	450, 642, 729, 775, 872
RsaNI	GTAC	5 cut(s)	449, 641, 728, 774, 871
RseI	CAYNNNNRTG	3 cut(s)	528, 729, 1035
SacI	GAGCTC	1 cut(s)	819
SalI	GTCGAC	1 cut(s)	230
SaqAI	TTAA	3 cut(s)	312, 809, 839
SatI	GCNGC	2 cut(s)	14, 28
Sau3AI	GATC	6 cut(s)	360, 553, 655, 802, 822, 1051
Sau96I	GGNCC	2 cut(s)	36, 602
ScaI	AGTACT	1 cut(s)	872
SchI	GAGTC	3 cut(s)	286, 527, 922
ScrFI	CCNGG	3 cut(s)	34, 100, 771
SduI	GDGCHC	2 cut(s)	105, 819
SfaNI	GCATC	4 cut(s)	129, 148, 256, 982
SinI	GGWCC	1 cut(s)	602
SmiMI	CAYNNNNRTG	3 cut(s)	528, 729, 1035
SmlI	CTYRAG	1 cut(s)	455
SmoI	CTYRAG	1 cut(s)	455
SnaBI	TACGTA	1 cut(s)	977
Sse9I	AATT	8 cut(s)	130, 491, 587, 883, 910, 934, 979, 1041
SspMI	CTAG	2 cut(s)	540, 599
SstI	GAGCTC	1 cut(s)	819
StyD4I	CCNGG	3 cut(s)	32, 98, 769
StyI	CCWWGG	1 cut(s)	781
TaaI	ACNGT	2 cut(s)	640, 830
TaiI	ACGT	4 cut(s)	733, 979, 1039, 1063
TaqI	TCGA	4 cut(s)	231, 419, 926, 1050
TasI	AATT	8 cut(s)	130, 491, 587, 883, 910, 934, 979, 1041
TatI	WGTACW	2 cut(s)	448, 870
TfiI	GAWTC	4 cut(s)	109, 403, 413, 749
Tru1I	TTAA	3 cut(s)	312, 809, 839
Tru9I	TTAA	3 cut(s)	312, 809, 839
TscAI	CASTG	1 cut(s)	690
TseFI	GTSAC	2 cut(s)	346, 896
TseI	GCWGC	2 cut(s)	13, 27
Tsp45I	GTSAC	2 cut(s)	346, 896
TspDTI	ATGAA	4 cut(s)	732, 762, 951, 1017
TspGWI	ACGGA	3 cut(s)	44, 270, 334
TspRI	CASTG	1 cut(s)	690
Tth111I	GACNNNGTC	1 cut(s)	1062
VpaK11BI	GGWCC	1 cut(s)	602
XapI	RAATTY	3 cut(s)	491, 587, 910
XbaI	TCTAGA	1 cut(s)	539
XceI	RCATGY	2 cut(s)	449, 728
XmiI	GTMKAC	1 cut(s)	231
XspI	CTAG	2 cut(s)	540, 599
ZraI	GACGTC	1 cut(s)	1061
ZrmI	AGTACT	1 cut(s)	872
Zsp2I	ATGCAT	1 cut(s)	726

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.