Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

17037848 .. 17045387

Gene Structure 7540 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGCTTGCGCCTAAGGGAGAGTTCTATAATTATTCGGGATGTGGTAACACATTCAATTGCAACCATCCTGTTGTGCGCCAGTTTATTGTAGACTGCTTGAGATATTGGGTAACAGAGATGCATGTGGATGGGTTCCGCTTTGATCTCGCTTCTATTATGACTAGGGGTAGCAGTCTCTGGAATGCAATTAATGTATATGGGAGTGCAATAGAAGGTGACTTGTTGACAACGGGAACCCCTCTTGCTACCCCACCATTGGTTGACATGATAAGTAATGACCCAATACTTCATGGAGTGAAGCTTATAGCTGAAGCATGGGATGCAGGAGGCCTGTACCAAGTTGGCATGTTTCCTCACTGGGGTAATTGGTCAGAATGGAATGGGAAGTATCGTGACACAGTGCGGCAGTTTATCAAGGGTACAGATGGCTTTTCTGGGGCTTTGGCTGAATGCCTTTGTGGGAGCCCCAACTTATATCAGGAAGGGGGGAGGCCATGGAGTAGTGTCAATTTTGTTTGTGCACACGATGGTTTTACTTTAGCTGATTTGGTAACCTATAACAACAAACATAACCTGGCAAATGGAGAAGACAACAATGACGGAGAGAGTCATAATAATAGCTGGAACTGTGGACAGGAGGGAGAGTTTGCAAGCATCTCAGTGAAGAAATTGAGGAAGCGACAAATGCGGAATTTTTTTGTCTGCCTCATGGTCTCCCAAGGTGTCCCAATGCTGTGTATGGGTGATGAATATGGCCATACAAAGGGAGGAAACAACAACACATATTGCCATGACAATTATATTAATTACTTTCGATGGGATAAGAAAGAAGAGTCCTCATCAGACTTTTTCAGATTTTGCTGCCTCATGACCAAATTCCGCCAGGAATGCGAGTCGCTTGGGTTAAATGACTTCCCAACGGCAGAGAGGCTGCAGTGGCATGGTCATGCTCCAGGGGTGCCAGACTGGTCTGACACAAGCCGCTTTGTTGCCTTTACTCTGGTGGACTCCGTGAAGAGAGAGCTGTATATTGCTTTCAATGCCAGCCACTTAGCCGTGACCATTTCTCTGCCAGAGAGGCCTGGATACAGATGGGAGCCCTTGGTCGACACCAGCAAGTGCACACCATTTGACTTCCTTTCTAGTGACGTCCCAGAAAGAGATACCGCAGTTAAGCAGTATGCTCACTTTCTCGACGCCAATTTATATCCCATGCTAAGTTATTCTTCAATCATCCTATTACTCTCTCCCGTTGAAGATTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

422

Amino Acids

47.55

Weight (kDa)

5.29

Isoelectric Point (pI)

37.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ISOA1-3_C	PF21156	312 - 413	2.7e-47	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1152
AccB1I	GGYRCC	1 cut(s)	958
AccI	GTMKAC	2 cut(s)	90, 1107
AciI	CCGC	6 cut(s)	136, 403, 688, 880, 982, 1167
AcoI	YGGCCR	1 cut(s)	754
AcsI	RAATTY	2 cut(s)	691, 875
AcuI	CTGAAG	1 cut(s)	330
AcyI	GRCGYC	2 cut(s)	1149, 1197
AfaI	GTAC	2 cut(s)	335, 421
AfiI	CCNNNNNNNGG	3 cut(s)	256, 359, 763
AgsI	TTSAA	4 cut(s)	55, 1039, 1230, 1256
AjnI	CCWGG	4 cut(s)	573, 882, 952, 1081
AluBI	AGCT	5 cut(s)	301, 308, 542, 621, 1024
AluI	AGCT	5 cut(s)	301, 308, 542, 621, 1024
Alw21I	GWGCWC	2 cut(s)	523, 1124
Alw26I	GTCTC	2 cut(s)	179, 718
Alw44I	GTGCAC	2 cut(s)	519, 1120
AlwNI	CAGNNNCTG	1 cut(s)	177
AoxI	GGCC	4 cut(s)	328, 491, 754, 1079
ApaLI	GTGCAC	2 cut(s)	519, 1120
ApeKI	GCWGC	2 cut(s)	861, 931
ApoI	RAATTY	2 cut(s)	691, 875
AseI	ATTAAT	2 cut(s)	189, 804
AspLEI	GCGC	2 cut(s)	10, 78
AsuHPI	GGTGA	2 cut(s)	227, 755
AxyI	CCTNAGG	1 cut(s)	12
BaeGI	GKGCMC	2 cut(s)	523, 1124
BaeI	ACNNNNGTAYC	2 cut(s)	1155, 1188
BalI	TGGCCA	1 cut(s)	756
BanI	GGYRCC	1 cut(s)	958
BanII	GRGCYC	2 cut(s)	467, 1101
BbsI	GAAGAC	1 cut(s)	594
Bbv12I	GWGCWC	2 cut(s)	523, 1124
BbvI	GCAGC	2 cut(s)	848, 918
BccI	CCATC	6 cut(s)	72, 122, 419, 521, 810, 1086
BceAI	ACGGC	2 cut(s)	936, 1040
BciT130I	CCWGG	4 cut(s)	575, 884, 954, 1083
BciVI	GTATCC	1 cut(s)	1079
BcoDI	GTCTC	2 cut(s)	179, 718
BfaI	CTAG	2 cut(s)	162, 1143
BfmI	CTRYAG	1 cut(s)	932
BfuI	GTATCC	1 cut(s)	1079
BglI	GCCNNNNNGGC	1 cut(s)	1078
BisI	GCNGC	4 cut(s)	404, 862, 932, 982
BlsI	GCNGC	4 cut(s)	405, 863, 933, 983
Bme1390I	CCNGG	4 cut(s)	575, 884, 954, 1083
BmiI	GGNNCC	5 cut(s)	134, 235, 464, 960, 1098
BmrFI	CCNGG	4 cut(s)	575, 884, 954, 1083
BmrI	ACTGGG	1 cut(s)	367
BmsI	GCATC	3 cut(s)	108, 310, 663
BmuI	ACTGGG	1 cut(s)	367
BpiI	GAAGAC	1 cut(s)	594
BpmI	CTGGAG	1 cut(s)	936
BpuEI	CTTGAG	1 cut(s)	118
BsaHI	GRCGYC	2 cut(s)	1149, 1197
BsaI	GGTCTC	1 cut(s)	718
BsaJI	CCNNGG	4 cut(s)	494, 718, 953, 1101
BsaXI	ACNNNNNCTCC	2 cut(s)	629, 659
Bsc4I	CCNNNNNNNGG	3 cut(s)	256, 359, 763
Bse1I	ACTGG	3 cut(s)	79, 362, 971
Bse21I	CCTNAGG	1 cut(s)	12
BseBI	CCWGG	4 cut(s)	575, 884, 954, 1083
BseDI	CCNNGG	4 cut(s)	494, 718, 953, 1101
BseGI	GGATG	5 cut(s)	44, 64, 133, 325, 1233
BseLI	CCNNNNNNNGG	3 cut(s)	256, 359, 763
BseMII	CTCAG	1 cut(s)	672
BseNI	ACTGG	3 cut(s)	79, 362, 971
BseSI	GKGCMC	2 cut(s)	523, 1124
BseXI	GCAGC	2 cut(s)	848, 918
BshFI	GGCC	4 cut(s)	330, 493, 756, 1081
BshNI	GGYRCC	1 cut(s)	958
BsiHKAI	GWGCWC	2 cut(s)	523, 1124
BslFI	GGGAC	2 cut(s)	710, 1136
BslI	CCNNNNNNNGG	3 cut(s)	256, 359, 763
BsmAI	GTCTC	2 cut(s)	179, 718
BsmFI	GGGAC	2 cut(s)	710, 1136
BsmI	GAATGC	3 cut(s)	187, 455, 893
BsnI	GGCC	4 cut(s)	330, 493, 756, 1081
Bso31I	GGTCTC	1 cut(s)	718
Bsp1286I	GDGCHC	4 cut(s)	467, 523, 1101, 1124
Bsp143I	GATC	1 cut(s)	142
Bsp19I	CCATGG	1 cut(s)	494
BspACI	CCGC	6 cut(s)	136, 403, 688, 880, 982, 1167
BspANI	GGCC	4 cut(s)	330, 493, 756, 1081
BspCNI	CTCAG	1 cut(s)	671
BspHI	TCATGA	1 cut(s)	867
BspLI	GGNNCC	5 cut(s)	134, 235, 464, 960, 1098
BspMAI	CTGCAG	1 cut(s)	936
BspT107I	GGYRCC	1 cut(s)	958
BspTNI	GGTCTC	1 cut(s)	718
BsrI	ACTGG	3 cut(s)	79, 362, 971
BssECI	CCNNGG	4 cut(s)	494, 718, 953, 1101
BssMI	GATC	1 cut(s)	142
BssNI	GRCGYC	2 cut(s)	1149, 1197
BssT1I	CCWWGG	3 cut(s)	494, 718, 1101
Bst2UI	CCWGG	4 cut(s)	575, 884, 954, 1083
Bst4CI	ACNGT	2 cut(s)	400, 629
Bst6I	CTCTTC	2 cut(s)	825, 1010
BstACI	GRCGYC	2 cut(s)	1149, 1197
BstAPI	GCANNNNNTGC	1 cut(s)	320
BstC8I	GCNNGC	3 cut(s)	6, 652, 1045
BstDEI	CTNAG	5 cut(s)	12, 658, 1051, 1217, 1263
BstDSI	CCRYGG	1 cut(s)	494
BstEII	GGTNACC	1 cut(s)	550
BstF5I	GGATG	5 cut(s)	44, 64, 133, 325, 1233
BstHHI	GCGC	2 cut(s)	10, 78
BstKTI	GATC	1 cut(s)	145
BstMAI	GTCTC	2 cut(s)	179, 718
BstMBI	GATC	1 cut(s)	142
BstMWI	GCNNNNNNNGC	6 cut(s)	320, 685, 888, 937, 1040, 1078
BstNI	CCWGG	4 cut(s)	575, 884, 954, 1083
BstNSI	RCATGY	2 cut(s)	125, 349
BstPI	GGTNACC	1 cut(s)	550
BstSCI	CCNGG	4 cut(s)	573, 882, 952, 1081
BstSFI	CTRYAG	1 cut(s)	932
BstSLI	GKGCMC	2 cut(s)	523, 1124
BstV1I	GCAGC	2 cut(s)	848, 918
BstV2I	GAAGAC	1 cut(s)	594
Bsu36I	CCTNAGG	1 cut(s)	12
BsuI	GTATCC	1 cut(s)	1079
BsuRI	GGCC	4 cut(s)	330, 493, 756, 1081
BtgI	CCRYGG	1 cut(s)	494
BtsCI	GGATG	5 cut(s)	44, 64, 133, 325, 1233
BtsI	GCAGTG	1 cut(s)	941
BtsIMutI	CAGTG	4 cut(s)	355, 405, 666, 941
Cac8I	GCNNGC	3 cut(s)	6, 652, 1045
CaiI	CAGNNNCTG	1 cut(s)	177
CciI	TCATGA	1 cut(s)	867
CfoI	GCGC	2 cut(s)	10, 78
CseI	GACGC	1 cut(s)	1205
Csp6I	GTAC	2 cut(s)	334, 420
CviQI	GTAC	2 cut(s)	334, 420
DdeI	CTNAG	5 cut(s)	12, 658, 1051, 1217, 1263
DpnI	GATC	1 cut(s)	144
DpnII	GATC	1 cut(s)	142
EaeI	YGGCCR	1 cut(s)	754
Eam1104I	CTCTTC	2 cut(s)	825, 1010
EarI	CTCTTC	2 cut(s)	825, 1010
EciI	GGCGGA	1 cut(s)	869
Eco130I	CCWWGG	3 cut(s)	494, 718, 1101
Eco147I	AGGCCT	2 cut(s)	330, 1081
Eco24I	GRGCYC	2 cut(s)	467, 1101
Eco31I	GGTCTC	1 cut(s)	718
Eco57I	CTGAAG	1 cut(s)	330
Eco81I	CCTNAGG	1 cut(s)	12
Eco91I	GGTNACC	1 cut(s)	550
EcoO65I	GGTNACC	1 cut(s)	550
EcoRII	CCWGG	4 cut(s)	573, 882, 952, 1081
EcoT14I	CCWWGG	3 cut(s)	494, 718, 1101
EcoT22I	ATGCAT	1 cut(s)	123
EcoT38I	GRGCYC	2 cut(s)	467, 1101
ErhI	CCWWGG	3 cut(s)	494, 718, 1101
FalI	AAGNNNNNCTT	2 cut(s)	1210, 1242
FaqI	GGGAC	2 cut(s)	710, 1136
FblI	GTMKAC	2 cut(s)	90, 1107
Fnu4HI	GCNGC	4 cut(s)	404, 862, 932, 982
FokI	GGATG	5 cut(s)	51, 51, 140, 332, 1220
FriOI	GRGCYC	2 cut(s)	467, 1101
Fsp4HI	GCNGC	4 cut(s)	404, 862, 932, 982
FspBI	CTAG	2 cut(s)	162, 1143
GlaI	GCGC	2 cut(s)	9, 77
GluI	GCNGC	4 cut(s)	404, 862, 932, 982
GsuI	CTGGAG	1 cut(s)	936
HaeIII	GGCC	4 cut(s)	330, 493, 756, 1081
HgaI	GACGC	1 cut(s)	1205
HhaI	GCGC	2 cut(s)	10, 78
Hin1I	GRCGYC	2 cut(s)	1149, 1197
Hin6I	GCGC	2 cut(s)	8, 76
HinP1I	GCGC	2 cut(s)	8, 76
HincII	GTYRAC	3 cut(s)	225, 262, 1108
HindII	GTYRAC	3 cut(s)	225, 262, 1108
HindIII	AAGCTT	1 cut(s)	299
HinfI	GANTC	5 cut(s)	607, 833, 893, 1007, 1259
HphI	GGTGA	2 cut(s)	227, 755
Hpy166II	GTNNAC	8 cut(s)	91, 225, 262, 521, 632, 1006, 1108, 1122
Hpy188I	TCNGA	4 cut(s)	373, 844, 854, 973
Hpy188III	TCNNGA	6 cut(s)	36, 178, 392, 479, 868, 1193
Hpy8I	GTNNAC	8 cut(s)	91, 225, 262, 521, 632, 1006, 1108, 1122
Hpy99I	CGWCG	1 cut(s)	1199
HpyAV	CCTTC	2 cut(s)	206, 476
HpyCH4III	ACNGT	2 cut(s)	400, 629
HpyCH4IV	ACGT	1 cut(s)	1149
HpyCH4V	TGCA	9 cut(s)	60, 121, 185, 206, 323, 521, 650, 934, 1122
HpyF10VI	GCNNNNNNNGC	6 cut(s)	320, 685, 888, 937, 1040, 1078
HpyF3I	CTNAG	5 cut(s)	12, 658, 1051, 1217, 1263
HpySE526I	ACGT	1 cut(s)	1149
Hsp92I	GRCGYC	2 cut(s)	1149, 1197
HspAI	GCGC	2 cut(s)	8, 76
Kzo9I	GATC	1 cut(s)	142
LmnI	GCTCC	3 cut(s)	462, 955, 1096
Lsp1109I	GCAGC	2 cut(s)	848, 918
LweI	GCATC	3 cut(s)	108, 310, 663
MaeI	CTAG	2 cut(s)	162, 1143
MaeII	ACGT	1 cut(s)	1149
MaeIII	GTNAC	7 cut(s)	44, 109, 215, 392, 550, 1057, 1145
MalI	GATC	1 cut(s)	144
MboI	GATC	1 cut(s)	142
MboII	GAAGA	5 cut(s)	599, 676, 842, 1027, 1218
MfeI	CAATTG	1 cut(s)	55
MhlI	GDGCHC	4 cut(s)	467, 523, 1101, 1124
MlsI	TGGCCA	1 cut(s)	756
MluNI	TGGCCA	1 cut(s)	756
MlyI	GAGTC	4 cut(s)	616, 842, 902, 1001
Mox20I	TGGCCA	1 cut(s)	756
Mph1103I	ATGCAT	1 cut(s)	123
MscI	TGGCCA	1 cut(s)	756
MseI	TTAA	4 cut(s)	189, 804, 905, 1173
MslI	CAYNNNNRTG	3 cut(s)	126, 659, 945
Msp20I	TGGCCA	1 cut(s)	756
MspR9I	CCNGG	4 cut(s)	575, 884, 954, 1083
MunI	CAATTG	1 cut(s)	55
Mva1269I	GAATGC	3 cut(s)	187, 455, 893
MvaI	CCWGG	4 cut(s)	575, 884, 954, 1083
MwoI	GCNNNNNNNGC	6 cut(s)	320, 685, 888, 937, 1040, 1078
NcoI	CCATGG	1 cut(s)	494
NdeII	GATC	1 cut(s)	142
NlaIV	GGNNCC	5 cut(s)	134, 235, 464, 960, 1098
NmuCI	GTSAC	4 cut(s)	215, 392, 1057, 1145
NsiI	ATGCAT	1 cut(s)	123
NspI	RCATGY	2 cut(s)	125, 349
PagI	TCATGA	1 cut(s)	867
PceI	AGGCCT	2 cut(s)	330, 1081
PctI	GAATGC	3 cut(s)	187, 455, 893
PfeI	GAWTC	1 cut(s)	1259
PkrI	GCNGC	4 cut(s)	405, 863, 933, 983
PleI	GAGTC	4 cut(s)	615, 841, 901, 1001
PpsI	GAGTC	4 cut(s)	615, 841, 901, 1001
PshBI	ATTAAT	2 cut(s)	189, 804
Psp6I	CCWGG	4 cut(s)	573, 882, 952, 1081
PspEI	GGTNACC	1 cut(s)	550
PspGI	CCWGG	4 cut(s)	573, 882, 952, 1081
PspN4I	GGNNCC	5 cut(s)	134, 235, 464, 960, 1098
PstI	CTGCAG	1 cut(s)	936
PstNI	CAGNNNCTG	1 cut(s)	177
RsaI	GTAC	2 cut(s)	335, 421
RsaNI	GTAC	2 cut(s)	334, 420
RseI	CAYNNNNRTG	3 cut(s)	126, 659, 945
SalI	GTCGAC	1 cut(s)	1106
SaqAI	TTAA	4 cut(s)	189, 804, 905, 1173
SatI	GCNGC	4 cut(s)	404, 862, 932, 982
Sau3AI	GATC	1 cut(s)	142
SchI	GAGTC	4 cut(s)	616, 842, 902, 1001
ScrFI	CCNGG	4 cut(s)	575, 884, 954, 1083
SduI	GDGCHC	4 cut(s)	467, 523, 1101, 1124
SfaNI	GCATC	3 cut(s)	108, 310, 663
SfcI	CTRYAG	1 cut(s)	932
SmiMI	CAYNNNNRTG	3 cut(s)	126, 659, 945
SmlI	CTYRAG	1 cut(s)	97
SmoI	CTYRAG	1 cut(s)	97
SseBI	AGGCCT	2 cut(s)	330, 1081
SsiI	CCGC	6 cut(s)	136, 403, 688, 880, 982, 1167
SspMI	CTAG	2 cut(s)	162, 1143
StuI	AGGCCT	2 cut(s)	330, 1081
StyD4I	CCNGG	4 cut(s)	573, 882, 952, 1081
StyI	CCWWGG	3 cut(s)	494, 718, 1101
TaaI	ACNGT	2 cut(s)	400, 629
TaiI	ACGT	1 cut(s)	1152
TaqI	TCGA	3 cut(s)	814, 1107, 1194
TauI	GCSGC	2 cut(s)	406, 984
TfiI	GAWTC	1 cut(s)	1259
Tru1I	TTAA	4 cut(s)	189, 804, 905, 1173
Tru9I	TTAA	4 cut(s)	189, 804, 905, 1173
TscAI	CASTG	4 cut(s)	362, 405, 666, 941
TseFI	GTSAC	4 cut(s)	215, 392, 1057, 1145
TseI	GCWGC	2 cut(s)	861, 931
Tsp45I	GTSAC	4 cut(s)	215, 392, 1057, 1145
TspDTI	ATGAA	2 cut(s)	278, 762
TspGWI	ACGGA	2 cut(s)	615, 1000
TspRI	CASTG	4 cut(s)	362, 405, 666, 941
VneI	GTGCAC	2 cut(s)	519, 1120
VspI	ATTAAT	2 cut(s)	189, 804
XapI	RAATTY	2 cut(s)	691, 875
XceI	RCATGY	2 cut(s)	125, 349
XmiI	GTMKAC	2 cut(s)	90, 1107
XspI	CTAG	2 cut(s)	162, 1143
ZraI	GACGTC	1 cut(s)	1150
Zsp2I	ATGCAT	1 cut(s)	123

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.