pycom14g01740

Protein BUD31 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003415
Physical Location & Seq
Reverse (-)
1277248 .. 1279077
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 588 bp
ATGTCGTCCATGGAATCTAACGGTCGTGTCGGGAGACCGCAGATATATTTCGAAAACCCTAAACCCCCTTTCCGCTCTGCTCCGCCTGTCTTCCCGCCACCTTCACCGCCTCCGCCACCACAGCGTTCTCTTCTCTCCATCAGGTATAAAATGCCGAAGGTGAAGACTAACCGAGTCAATTACCCAGAAGGTTGGGAACTGATTGAGCCTACGCTACGCGAACTACAAGCTAAGATGAGAGAAGCCGAGAATGATACCCATGATGGTAAGAGAAAATGTGAGACCTTGTGGCCTATTTTCAAAGTTGCACATCAAAAGAGCCGCTACATTTTTGACCTTTACCACCGGAGAAATGAAATTTCCAAGGAGTTATATGAGTTTTGCCTGGACCAAGGCTATGCTGACCGCAATCTAATTGCTAAATGGAAAAAGCCTGGTTATGAACGGCTCTGCTGTTTAAGATGCATGCAGCCCCGGGACCATAACTTTGCCACCACATGCGTTTGCCGAGTTCCCAAGCATCTGAGGGAGGAGAAGGTTATAGAGTGTGTGCACTGTGGCTGTCGGGGCTGTGCCAGTGGAGATTGA

Protein Analysis

196

Amino Acids

22.84

Weight (kDa)

9.21

Isoelectric Point (pI)

63.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BUD31 PF01125 51 - 193 2.4e-64 Pre-mRNA-splicing factor BUD31
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 75
AccII CGCG 1 cut(s) 219
AciI CCGC 8 cut(s) 38, 73, 83, 95, 107, 113, 322, 406
AcsI RAATTY 1 cut(s) 357
AgsI TTSAA 1 cut(s) 301
AjnI CCWGG 2 cut(s) 384, 433
AluBI AGCT 1 cut(s) 230
AluI AGCT 1 cut(s) 230
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 2 cut(s) 28, 275
Alw44I GTGCAC 1 cut(s) 551
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 1 cut(s) 290
ApaLI GTGCAC 1 cut(s) 551
ApeKI GCWGC 1 cut(s) 469
ApoI RAATTY 1 cut(s) 357
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
AspS9I GGNCC 2 cut(s) 388, 478
AsuC2I CCSGG 2 cut(s) 475, 476
AsuHPI GGTGA 2 cut(s) 96, 172
AsuII TTCGAA 1 cut(s) 51
AvaI CYCGRG 1 cut(s) 474
AvaII GGWCC 2 cut(s) 388, 478
BaeGI GKGCMC 1 cut(s) 555
BbsI GAAGAC 2 cut(s) 82, 170
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 1 cut(s) 481
BccI CCATC 2 cut(s) 146, 257
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 2 cut(s) 386, 435
BcnI CCSGG 2 cut(s) 475, 476
BcoDI GTCTC 2 cut(s) 28, 275
BisI GCNGC 2 cut(s) 322, 470
BlsI GCNGC 2 cut(s) 323, 471
Bme1390I CCNGG 4 cut(s) 386, 435, 475, 476
Bme18I GGWCC 2 cut(s) 388, 478
BmeT110I CYCGRG 1 cut(s) 474
BmgT120I GGNCC 2 cut(s) 388, 478
BmiI GGNNCC 1 cut(s) 479
BmrFI CCNGG 4 cut(s) 386, 435, 475, 476
BmsI GCATC 2 cut(s) 452, 529
BpiI GAAGAC 2 cut(s) 82, 170
Bpu14I TTCGAA 1 cut(s) 51
BpuMI CCSGG 2 cut(s) 475, 476
BsaI GGTCTC 2 cut(s) 28, 275
BsaJI CCNNGG 5 cut(s) 9, 363, 391, 473, 474
BsaWI WCCGGW 1 cut(s) 345
Bse1I ACTGG 1 cut(s) 576
BseBI CCWGG 2 cut(s) 386, 435
BseDI CCNNGG 5 cut(s) 9, 363, 391, 473, 474
BseMII CTCAG 1 cut(s) 515
BseNI ACTGG 1 cut(s) 576
BseRI GAGGAG 1 cut(s) 545
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 1 cut(s) 481
Bsh1236I CGCG 1 cut(s) 219
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 1 cut(s) 292
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 555
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 2 cut(s) 346, 475
BslFI GGGAC 1 cut(s) 491
BsmAI GTCTC 2 cut(s) 28, 275
BsmFI GGGAC 1 cut(s) 491
BsnI GGCC 1 cut(s) 292
Bso31I GGTCTC 2 cut(s) 28, 275
BsoBI CYCGRG 1 cut(s) 474
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 555
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 8 cut(s) 38, 73, 83, 95, 107, 113, 322, 406
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 1 cut(s) 516
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 479
BspT104I TTCGAA 1 cut(s) 51
BspTNI GGTCTC 2 cut(s) 28, 275
BsrBI CCGCTC 1 cut(s) 75
BsrI ACTGG 1 cut(s) 576
BssECI CCNNGG 5 cut(s) 9, 363, 391, 473, 474
BssT1I CCWWGG 3 cut(s) 9, 363, 391
Bst2UI CCWGG 2 cut(s) 386, 435
Bst4CI ACNGT 2 cut(s) 23, 557
Bst6I CTCTTC 1 cut(s) 135
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 467
BstDEI CTNAG 2 cut(s) 231, 524
BstDSI CCRYGG 1 cut(s) 9
BstFNI CGCG 1 cut(s) 219
BstMAI GTCTC 2 cut(s) 28, 275
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 2 cut(s) 121, 567
BstNI CCWGG 2 cut(s) 386, 435
BstNSI RCATGY 2 cut(s) 469, 501
BstSCI CCNGG 4 cut(s) 384, 433, 473, 474
BstSLI GKGCMC 1 cut(s) 555
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 481
BstV2I GAAGAC 2 cut(s) 82, 170
BstXI CCANNNNNNTGG 1 cut(s) 192
BsuRI GGCC 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 9
BtsIMutI CAGTG 2 cut(s) 553, 583
Cac8I GCNNGC 1 cut(s) 467
Cfr13I GGNCC 2 cut(s) 388, 478
Cfr9I CCCGGG 1 cut(s) 474
CviAII CATG 4 cut(s) 10, 260, 466, 498
DdeI CTNAG 2 cut(s) 231, 524
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
EciI GGCGGA 2 cut(s) 72, 102
Eco130I CCWWGG 3 cut(s) 9, 363, 391
Eco31I GGTCTC 2 cut(s) 28, 275
Eco47I GGWCC 2 cut(s) 388, 478
Eco88I CYCGRG 1 cut(s) 474
EcoRII CCWGG 2 cut(s) 384, 433
EcoT14I CCWWGG 3 cut(s) 9, 363, 391
EcoT22I ATGCAT 1 cut(s) 467
ErhI CCWWGG 3 cut(s) 9, 363, 391
FaeI CATG 4 cut(s) 13, 263, 469, 501
FaqI GGGAC 1 cut(s) 491
FatI CATG 4 cut(s) 9, 259, 465, 497
FauI CCCGC 1 cut(s) 102
Fnu4HI GCNGC 2 cut(s) 322, 470
Fsp4HI GCNGC 2 cut(s) 322, 470
GluI GCNGC 2 cut(s) 322, 470
HaeIII GGCC 1 cut(s) 292
HapII CCGG 2 cut(s) 346, 475
Hin1II CATG 4 cut(s) 13, 263, 469, 501
HinfI GANTC 2 cut(s) 14, 174
HpaII CCGG 2 cut(s) 346, 475
HphI GGTGA 2 cut(s) 96, 172
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 1 cut(s) 525
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 1 cut(s) 553
HpyAV CCTTC 4 cut(s) 111, 151, 182, 529
HpyCH4III ACNGT 2 cut(s) 23, 557
HpyCH4V TGCA 4 cut(s) 308, 465, 469, 553
HpyF10VI GCNNNNNNNGC 2 cut(s) 121, 567
HpyF3I CTNAG 2 cut(s) 231, 524
Hsp92II CATG 4 cut(s) 13, 263, 469, 501
LmnI GCTCC 1 cut(s) 85
LpnPI CCDG 9 cut(s) 99, 127, 198, 359, 371, 398, 420, 447, 488
Lsp1109I GCAGC 1 cut(s) 481
LweI GCATC 2 cut(s) 452, 529
MbiI CCGCTC 1 cut(s) 75
MboII GAAGA 3 cut(s) 82, 122, 175
MhlI GDGCHC 1 cut(s) 555
MluCI AATT 3 cut(s) 178, 357, 414
MlyI GAGTC 1 cut(s) 183
MnlI CCTC 3 cut(s) 120, 519, 523
Mph1103I ATGCAT 1 cut(s) 467
MseI TTAA 1 cut(s) 458
MspI CCGG 2 cut(s) 346, 475
MspR9I CCNGG 4 cut(s) 386, 435, 475, 476
MvaI CCWGG 2 cut(s) 386, 435
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 2 cut(s) 121, 567
NciI CCSGG 2 cut(s) 475, 476
NcoI CCATGG 1 cut(s) 9
NlaIII CATG 4 cut(s) 13, 263, 469, 501
NlaIV GGNNCC 1 cut(s) 479
NmeAIII GCCGAG 2 cut(s) 271, 533
NsiI ATGCAT 1 cut(s) 467
NspI RCATGY 2 cut(s) 469, 501
NspV TTCGAA 1 cut(s) 51
PaeI GCATGC 1 cut(s) 469
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 2 cut(s) 323, 471
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Psp6I CCWGG 2 cut(s) 384, 433
PspGI CCWGG 2 cut(s) 384, 433
PspN4I GGNNCC 1 cut(s) 479
PspPI GGNCC 2 cut(s) 388, 478
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 2 cut(s) 322, 470
Sau96I GGNCC 2 cut(s) 388, 478
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 4 cut(s) 386, 435, 475, 476
SduI GDGCHC 1 cut(s) 555
SetI ASST 8 cut(s) 103, 146, 162, 193, 232, 287, 339, 540
SfaNI GCATC 2 cut(s) 452, 529
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 2 cut(s) 388, 478
SmaI CCCGGG 1 cut(s) 476
SphI GCATGC 1 cut(s) 469
Sse9I AATT 3 cut(s) 178, 357, 414
SsiI CCGC 8 cut(s) 38, 73, 83, 95, 107, 113, 322, 406
StyD4I CCNGG 4 cut(s) 384, 433, 473, 474
StyI CCWWGG 3 cut(s) 9, 363, 391
TaaI ACNGT 2 cut(s) 23, 557
TaqI TCGA 1 cut(s) 51
TasI AATT 3 cut(s) 178, 357, 414
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TscAI CASTG 2 cut(s) 560, 583
TseI GCWGC 1 cut(s) 469
TspDTI ATGAA 2 cut(s) 369, 456
TspMI CCCGGG 1 cut(s) 474
TspRI CASTG 2 cut(s) 560, 583
VneI GTGCAC 1 cut(s) 551
VpaK11BI GGWCC 2 cut(s) 388, 478
XapI RAATTY 1 cut(s) 357
XceI RCATGY 2 cut(s) 469, 501
XmaI CCCGGG 1 cut(s) 474
Zsp2I ATGCAT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.