pycom14g13020

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007112
Physical Location & Seq
Reverse (-)
16042312 .. 16044414
2103 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1317 bp
ATGCAGGAGAAGAAGAAGGAGATTACGAAGAAGAAGAAGAAGAAGTACGAGTTCGAGTACTGCGTGGTTTGCAAGCTGAATCACGACCAAGGTCCGAGCCACAAGTACACACCCAGCCACACCAAGTCCCTCTCCGCCTTCCTTACTCGCTTCCAAGCCAAGCTCTCCGATGTCCGCTTCTTTCTCAAAAACCCTATCCCTCTCCGCCCCGAACACGCCTCCCGCAACCGCCTCTGGTGCGTTTTCTGCGAGTCCGACGTCGACGAGCTCGATAGCTCCTTCGCCTGTGGTAATGCAATTAATCACCTTGCGAACGCGGAACATCTGAAGCATTTGAAGCATTTCTTGTGGAAATACGGTGGAGGAATGTACCGCGTCGATACATTCAGAATTTCGGAAGATGAACTTGCTAAGTGGGAGAAGAAGTGTAATTCATTGAAGCTTGAAGCTGTGCCATGTGGTGAAGGATCTCGAGGACCAACATTTGGACCATCGAATGATATCCATACTGAACTGAAATACGGAATTATAGATACTTTTGAAAATAATAGTAGTTCAAGTTTTTCACATGGTGTTATGCCTTTACTATATCATACAAATGAGTATCAGGTATCCCATTCAGGACTCCCTCAAGTTACAAATGTTGGCTGCTTTTCGCAAGATGTTGCTACCTCTTCTTATCATGGGGGATCAAGTTCTGGTACAAATTCCATAAATTCAAACGCTTTAACAGTTATCAGGAGTAACCAACATTCTTTGGTTTCTAATGGTAGAAAATCCTCGGCTGATGGTTACTTCAGTAATGAAAGGATGAGAGAGGGGTCTCAGCATGAAAGAATGACAAACGAGCAGAGCAGTTCTCCAGTTTTTCAGAATCTCACCCAAATATCGTCCATGGGTCCTAAAGAGGCTAGTGGAAATGTGCACACTGGGGCACCTCCTCCTTGGCTTCAAGCAGATGAAGAAATTCAGTGTAGTGTTCCACTGAAACGAGGCAGCCTTATTCCCCCGTCAAATAAGTTGGGGAAGTCCAAGAAATTGAACCCAAACCGTGTGGGAGCAGCTTGGGCAGAAAGGAGAAAGAGGGAGATGGAGATGGAGAAAAGAGGGGAAATTGTCATGAGTGACTGTGATGCCAGTTGGCTCCCTAATTTTGGTAGAGTTTGGCAGTCCGGTAGCCGAAAGGAGTCCAGAAAAGAATTTGAGTTGGAGAAACAAAAATTACCTAAGGTTGAAAGTCAGTCGGATTTGCCAATTAAGATACAGCCTTACATCAGCAAACGAATGGTGAGTTCCATGTTCCATCTTAGCATGTAA

Protein Analysis

439

Amino Acids

49.47

Weight (kDa)

9.15

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC84 PF14968 20 - 407 4.9e-94 Coiled coil protein 84
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 934
AccB7I CCANNNNNTGG 1 cut(s) 485
AccI GTMKAC 1 cut(s) 261
AccII CGCG 2 cut(s) 317, 375
AciI CCGC 7 cut(s) 135, 175, 205, 223, 229, 317, 373
AclWI GGATC 2 cut(s) 475, 697
AcsI RAATTY 5 cut(s) 390, 706, 715, 966, 1199
AcuI CTGAAG 2 cut(s) 347, 781
AcyI GRCGYC 1 cut(s) 258
AdeI CACNNNGTG 1 cut(s) 572
AfaI GTAC 5 cut(s) 47, 59, 107, 371, 703
AfiI CCNNNNNNNGG 2 cut(s) 485, 1154
AgsI TTSAA 9 cut(s) 337, 439, 446, 542, 558, 720, 953, 1042, 1235
AluBI AGCT 7 cut(s) 76, 163, 268, 276, 442, 449, 1064
AluI AGCT 7 cut(s) 76, 163, 268, 276, 442, 449, 1064
Alw21I GWGCWC 2 cut(s) 270, 927
Alw26I GTCTC 1 cut(s) 828
Alw44I GTGCAC 1 cut(s) 923
AlwI GGATC 2 cut(s) 475, 697
Ama87I CYCGRG 1 cut(s) 471
ApaLI GTGCAC 1 cut(s) 923
ApeKI GCWGC 3 cut(s) 648, 996, 1061
ApoI RAATTY 5 cut(s) 390, 706, 715, 966, 1199
AseI ATTAAT 1 cut(s) 300
Asp700I GAANNNNTTC 2 cut(s) 341, 966
AspS9I GGNCC 4 cut(s) 92, 476, 488, 899
AsuHPI GGTGA 4 cut(s) 296, 473, 871, 1300
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 4 cut(s) 92, 476, 488, 899
AxyI CCTNAGG 1 cut(s) 1227
BaeGI GKGCMC 2 cut(s) 927, 937
BanI GGYRCC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 270
BarI GAAGNNNNNNTAC 2 cut(s) 29, 61
Bbv12I GWGCWC 2 cut(s) 270, 927
BbvI GCAGC 3 cut(s) 635, 1008, 1073
BccI CCATC 5 cut(s) 499, 782, 1084, 1090, 1311
BciVI GTATCC 1 cut(s) 622
BcoDI GTCTC 1 cut(s) 828
BfaI CTAG 1 cut(s) 912
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 3 cut(s) 649, 997, 1062
BlsI GCNGC 3 cut(s) 650, 998, 1063
BmcAI AGTACT 1 cut(s) 59
Bme18I GGWCC 4 cut(s) 92, 476, 488, 899
BmeT110I CYCGRG 1 cut(s) 471
BmgT120I GGNCC 4 cut(s) 92, 476, 488, 899
BmiI GGNNCC 3 cut(s) 900, 936, 1145
BmrI ACTGGG 1 cut(s) 939
BmsI GCATC 1 cut(s) 1123
BmuI ACTGGG 1 cut(s) 939
BoxI GACNNNNGTC 1 cut(s) 90
BplI GAGNNNNNCTC 2 cut(s) 844, 876
BpmI CTGGAG 1 cut(s) 846
BpuEI CTTGAG 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 828
BsaJI CCNNGG 4 cut(s) 88, 780, 894, 944
BsaWI WCCGGW 1 cut(s) 1172
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 2 cut(s) 485, 1154
Bse1I ACTGG 3 cut(s) 863, 934, 1137
Bse21I CCTNAGG 1 cut(s) 1227
BseDI CCNNGG 4 cut(s) 88, 780, 894, 944
BseGI GGATG 1 cut(s) 816
BseLI CCNNNNNNNGG 2 cut(s) 485, 1154
BseMII CTCAG 1 cut(s) 839
BseNI ACTGG 3 cut(s) 863, 934, 1137
BseRI GAGGAG 1 cut(s) 930
BseSI GKGCMC 2 cut(s) 927, 937
BseXI GCAGC 3 cut(s) 635, 1008, 1073
BseYI CCCAGC 1 cut(s) 113
Bsh1236I CGCG 2 cut(s) 317, 375
BshNI GGYRCC 1 cut(s) 934
BsiHKAI GWGCWC 2 cut(s) 270, 927
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 1 cut(s) 1173
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 2 cut(s) 485, 1154
BsmAI GTCTC 1 cut(s) 828
BsmFI GGGAC 1 cut(s) 112
Bso31I GGTCTC 1 cut(s) 828
BsoBI CYCGRG 1 cut(s) 471
Bsp1286I GDGCHC 3 cut(s) 270, 927, 937
Bsp143I GATC 2 cut(s) 467, 689
Bsp19I CCATGG 1 cut(s) 894
BspACI CCGC 7 cut(s) 135, 175, 205, 223, 229, 317, 373
BspCNI CTCAG 1 cut(s) 838
BspFNI CGCG 2 cut(s) 317, 375
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 3 cut(s) 900, 936, 1145
BspPI GGATC 2 cut(s) 475, 697
BspT107I GGYRCC 1 cut(s) 934
BspTNI GGTCTC 1 cut(s) 828
BsrI ACTGG 3 cut(s) 863, 934, 1137
BssECI CCNNGG 4 cut(s) 88, 780, 894, 944
BssMI GATC 2 cut(s) 467, 689
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 3 cut(s) 88, 894, 944
Bst4CI ACNGT 4 cut(s) 359, 733, 1052, 1130
Bst6I CTCTTC 1 cut(s) 679
BstACI GRCGYC 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 74
BstDEI CTNAG 4 cut(s) 411, 825, 1227, 1307
BstDSI CCRYGG 1 cut(s) 894
BstF5I GGATG 1 cut(s) 816
BstFNI CGCG 2 cut(s) 317, 375
BstKTI GATC 2 cut(s) 470, 692
BstMAI GTCTC 1 cut(s) 828
BstMBI GATC 2 cut(s) 467, 689
BstMWI GCNNNNNNNGC 5 cut(s) 69, 237, 246, 337, 1067
BstNSI RCATGY 1 cut(s) 1315
BstPAI GACNNNNGTC 1 cut(s) 90
BstSLI GKGCMC 2 cut(s) 927, 937
BstUI CGCG 2 cut(s) 317, 375
BstV1I GCAGC 3 cut(s) 635, 1008, 1073
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
Bsu36I CCTNAGG 1 cut(s) 1227
BsuI GTATCC 1 cut(s) 622
BtgI CCRYGG 1 cut(s) 894
BtsCI GGATG 1 cut(s) 816
BtsIMutI CAGTG 3 cut(s) 927, 977, 983
Cac8I GCNNGC 1 cut(s) 74
CciI TCATGA 1 cut(s) 1119
Cfr13I GGNCC 4 cut(s) 92, 476, 488, 899
CseI GACGC 1 cut(s) 364
Csp6I GTAC 5 cut(s) 46, 58, 106, 370, 702
CspCI CAANNNNNGTGG 2 cut(s) 1035, 1070
CviAII CATG 8 cut(s) 456, 569, 683, 830, 895, 1120, 1297, 1312
CviQI GTAC 5 cut(s) 46, 58, 106, 370, 702
DdeI CTNAG 4 cut(s) 411, 825, 1227, 1307
DpnI GATC 2 cut(s) 469, 691
DpnII GATC 2 cut(s) 467, 689
DraIII CACNNNGTG 1 cut(s) 572
Eam1104I CTCTTC 1 cut(s) 679
EarI CTCTTC 1 cut(s) 679
EciI GGCGGA 2 cut(s) 124, 194
Ecl136II GAGCTC 1 cut(s) 268
Eco130I CCWWGG 3 cut(s) 88, 894, 944
Eco24I GRGCYC 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 828
Eco32I GATATC 1 cut(s) 502
Eco47I GGWCC 4 cut(s) 92, 476, 488, 899
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 2 cut(s) 347, 781
Eco81I CCTNAGG 1 cut(s) 1227
Eco88I CYCGRG 1 cut(s) 471
EcoICRI GAGCTC 1 cut(s) 268
EcoO109I RGGNCCY 1 cut(s) 899
EcoRV GATATC 1 cut(s) 502
EcoT14I CCWWGG 3 cut(s) 88, 894, 944
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 3 cut(s) 88, 894, 944
FaeI CATG 8 cut(s) 459, 572, 686, 833, 898, 1123, 1300, 1315
FalI AAGNNNNNCTT 4 cut(s) 329, 361, 390, 422
FaqI GGGAC 1 cut(s) 112
FatI CATG 8 cut(s) 455, 568, 682, 829, 894, 1119, 1296, 1311
FauI CCCGC 1 cut(s) 230
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 3 cut(s) 649, 997, 1062
FokI GGATG 1 cut(s) 823
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 3 cut(s) 649, 997, 1062
FspBI CTAG 1 cut(s) 912
GluI GCNGC 3 cut(s) 649, 997, 1062
GsaI CCCAGC 1 cut(s) 117
GsuI CTGGAG 1 cut(s) 846
HapII CCGG 1 cut(s) 1173
HgaI GACGC 1 cut(s) 364
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 8 cut(s) 459, 572, 686, 833, 898, 1123, 1300, 1315
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 5 cut(s) 79, 251, 624, 874, 1187
HpaII CCGG 1 cut(s) 1173
HphI GGTGA 4 cut(s) 296, 473, 871, 1300
Hpy166II GTNNAC 3 cut(s) 108, 262, 925
Hpy188I TCNGA 8 cut(s) 96, 169, 256, 327, 389, 397, 873, 1246
Hpy188III TCNNGA 6 cut(s) 83, 471, 621, 739, 1120, 1191
Hpy8I GTNNAC 3 cut(s) 108, 262, 925
Hpy99I CGWCG 4 cut(s) 260, 263, 266, 380
HpyAV CCTTC 4 cut(s) 10, 148, 289, 458
HpyCH4III ACNGT 4 cut(s) 359, 733, 1052, 1130
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 4 cut(s) 4, 72, 296, 925
HpyF10VI GCNNNNNNNGC 5 cut(s) 69, 237, 246, 337, 1067
HpyF3I CTNAG 4 cut(s) 411, 825, 1227, 1307
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 8 cut(s) 459, 572, 686, 833, 898, 1123, 1300, 1315
Kzo9I GATC 2 cut(s) 467, 689
LmnI GCTCC 3 cut(s) 281, 1058, 1149
Lsp1109I GCAGC 3 cut(s) 635, 1008, 1073
LweI GCATC 1 cut(s) 1123
MaeI CTAG 1 cut(s) 912
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 4 cut(s) 634, 743, 791, 1124
MalI GATC 2 cut(s) 469, 691
MboI GATC 2 cut(s) 467, 689
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 3 cut(s) 270, 927, 937
MlyI GAGTC 3 cut(s) 260, 618, 1196
MmeI TCCRAC 3 cut(s) 279, 1188, 1224
MroXI GAANNNNTTC 2 cut(s) 341, 966
MseI TTAA 3 cut(s) 300, 728, 1257
MslI CAYNNNNRTG 1 cut(s) 597
MspI CCGG 1 cut(s) 1173
MvnI CGCG 2 cut(s) 317, 375
MwoI GCNNNNNNNGC 5 cut(s) 69, 237, 246, 337, 1067
NcoI CCATGG 1 cut(s) 894
NdeII GATC 2 cut(s) 467, 689
NlaIII CATG 8 cut(s) 459, 572, 686, 833, 898, 1123, 1300, 1315
NlaIV GGNNCC 3 cut(s) 900, 936, 1145
NmeAIII GCCGAG 1 cut(s) 761
NmuCI GTSAC 1 cut(s) 1124
NspI RCATGY 1 cut(s) 1315
PaeR7I CTCGAG 1 cut(s) 471
PagI TCATGA 1 cut(s) 1119
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 267
PdmI GAANNNNTTC 2 cut(s) 341, 966
PfeI GAWTC 2 cut(s) 79, 874
PflMI CCANNNNNTGG 1 cut(s) 485
PkrI GCNGC 3 cut(s) 650, 998, 1063
PleI GAGTC 3 cut(s) 259, 618, 1195
PpsI GAGTC 3 cut(s) 259, 618, 1195
PpuMI RGGWCCY 1 cut(s) 899
PshAI GACNNNNGTC 1 cut(s) 90
PshBI ATTAAT 1 cut(s) 300
Psp124BI GAGCTC 1 cut(s) 270
Psp5II RGGWCCY 1 cut(s) 899
PspFI CCCAGC 1 cut(s) 113
PspN4I GGNNCC 3 cut(s) 900, 936, 1145
PspPI GGNCC 4 cut(s) 92, 476, 488, 899
PspPPI RGGWCCY 1 cut(s) 899
PsuI RGATCY 1 cut(s) 467
RsaI GTAC 5 cut(s) 47, 59, 107, 371, 703
RsaNI GTAC 5 cut(s) 46, 58, 106, 370, 702
RseI CAYNNNNRTG 1 cut(s) 597
SacI GAGCTC 1 cut(s) 270
SalI GTCGAC 1 cut(s) 260
SaqAI TTAA 3 cut(s) 300, 728, 1257
SatI GCNGC 3 cut(s) 649, 997, 1062
Sau3AI GATC 2 cut(s) 467, 689
Sau96I GGNCC 4 cut(s) 92, 476, 488, 899
ScaI AGTACT 1 cut(s) 59
SchI GAGTC 3 cut(s) 260, 618, 1196
SduI GDGCHC 3 cut(s) 270, 927, 937
SfaNI GCATC 1 cut(s) 1123
Sfr274I CTCGAG 1 cut(s) 471
SgrDI CGTCGACG 1 cut(s) 260
SinI GGWCC 4 cut(s) 92, 476, 488, 899
SlaI CTCGAG 1 cut(s) 471
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 2 cut(s) 471, 630
SmoI CTYRAG 2 cut(s) 471, 630
SsiI CCGC 7 cut(s) 135, 175, 205, 223, 229, 317, 373
SspMI CTAG 1 cut(s) 912
SstI GAGCTC 1 cut(s) 270
StyI CCWWGG 3 cut(s) 88, 894, 944
TaaI ACNGT 4 cut(s) 359, 733, 1052, 1130
TaiI ACGT 1 cut(s) 261
TaqI TCGA 6 cut(s) 54, 261, 270, 378, 472, 494
TatI WGTACW 2 cut(s) 57, 105
TfiI GAWTC 2 cut(s) 79, 874
Tru1I TTAA 3 cut(s) 300, 728, 1257
Tru9I TTAA 3 cut(s) 300, 728, 1257
TscAI CASTG 3 cut(s) 934, 977, 990
TseFI GTSAC 1 cut(s) 1124
TseI GCWGC 3 cut(s) 648, 996, 1061
Tsp45I GTSAC 1 cut(s) 1124
TspDTI ATGAA 5 cut(s) 417, 423, 819, 846, 975
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 3 cut(s) 934, 977, 990
Van91I CCANNNNNTGG 1 cut(s) 485
VneI GTGCAC 1 cut(s) 923
VpaK11BI GGWCC 4 cut(s) 92, 476, 488, 899
VspI ATTAAT 1 cut(s) 300
XapI RAATTY 5 cut(s) 390, 706, 715, 966, 1199
XceI RCATGY 1 cut(s) 1315
XhoI CTCGAG 1 cut(s) 471
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 2 cut(s) 341, 966
XspI CTAG 1 cut(s) 912
ZraI GACGTC 1 cut(s) 259
ZrmI AGTACT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.