pycom14g14860

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006145
Physical Location & Seq
Forward (+)
17531481 .. 17532894
1414 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 672 bp
ATGGAAGCTCCCGAAGACCCCGTCTCAAACGTAATGGATCATCCTTTGCTTTCTCCGAAACTATCAGGAACTGTCAACTGGGGCACGGCGACTGTCATCGGAGTGTTTGCAGGCTTGTTTTACGGTGGTAGCAAGGAAGCAGCTGCTTCAGTTTGTTGTAATCTTCGAAAAGATTGGATATCGACAGACATATTTGATTGGCAGAGCAAAGATGCAGAAGTAATGTTGAAGCTGGGGAGCACGCCAGACAAGCGTGAACAATATCGTCTCATGAGAGATGCAATGGAGAAAAGGTTCATTCGTGTCACTCGGGGCTCGATAGTTGGTGGAGTGCGTCTTGGATTGTTTACTGCAACCTTTTGTGGTATACAGAATCTGTTTGCCGAGAAAAGGGGCGTGCACGATGTTTTCAATGTTGTTGGAGCCGGATCAGCCACTGCTGCTACGTTTGCTCTAATAATGCCCGGATCATTGGCTTGGCGAGCAAGAAACGTGATGTTGGGTGCTGCCCTAGGGGCGGCGTTTGGTTTTCCACTTGGGTGGGCCCATTTGAAGCTGGTAGAGAAGGCAAATGAAGGGAATGCGGCTGCACATCCTGATTCAGATCAAAGAGAAGCAAGGAGTGGTGTTGGCGCTGCAATTGACAGGCTGGAAGAAAACTTGAATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

224

Amino Acids

24.0

Weight (kDa)

7.74

Isoelectric Point (pI)

23.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 29 - 175 5.8e-09 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 367
AciI CCGC 2 cut(s) 518, 584
AclWI GGATC 3 cut(s) 45, 436, 475
AcuI CTGAAG 1 cut(s) 132
AfiI CCNNNNNNNGG 2 cut(s) 390, 517
AgsI TTSAA 4 cut(s) 229, 412, 553, 664
AleI CACNNNNGTG 1 cut(s) 538
AloI GAACNNNNNNTCC 2 cut(s) 278, 310
AluBI AGCT 4 cut(s) 8, 143, 232, 556
AluI AGCT 4 cut(s) 8, 143, 232, 556
Alw21I GWGCWC 2 cut(s) 242, 402
Alw26I GTCTC 2 cut(s) 28, 272
Alw44I GTGCAC 1 cut(s) 398
AlwI GGATC 3 cut(s) 45, 436, 475
AlwNI CAGNNNCTG 3 cut(s) 71, 376, 437
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 1 cut(s) 543
ApaI GGGCCC 1 cut(s) 547
ApaLI GTGCAC 1 cut(s) 398
ApeKI GCWGC 6 cut(s) 140, 143, 440, 506, 587, 635
ArsI GACNNNNNNTTYG 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 293
AspA2I CCTAGG 1 cut(s) 511
AspLEI GCGC 1 cut(s) 635
AspS9I GGNCC 2 cut(s) 543, 544
AsuC2I CCSGG 1 cut(s) 465
AsuII TTCGAA 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 309
AvrII CCTAGG 1 cut(s) 511
BaeGI GKGCMC 3 cut(s) 86, 402, 547
BanII GRGCYC 2 cut(s) 317, 547
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 2 cut(s) 242, 402
BbvI GCAGC 6 cut(s) 130, 152, 427, 493, 574, 622
BceAI ACGGC 1 cut(s) 102
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 2 cut(s) 28, 272
BfaI CTAG 1 cut(s) 512
BfoI RGCGCY 1 cut(s) 636
BglI GCCNNNNNGGC 1 cut(s) 515
BisI GCNGC 8 cut(s) 141, 144, 441, 507, 519, 585, 588, 636
BlnI CCTAGG 1 cut(s) 511
BlsI GCNGC 8 cut(s) 142, 145, 442, 508, 520, 586, 589, 637
Bme1390I CCNGG 1 cut(s) 465
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 2 cut(s) 543, 544
BmiI GGNNCC 2 cut(s) 424, 545
BmrFI CCNGG 1 cut(s) 465
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 2 cut(s) 202, 268
BmuI ACTGGG 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 21
Bpu14I TTCGAA 1 cut(s) 166
BpuMI CCSGG 1 cut(s) 465
BsaBI GATNNNNATC 1 cut(s) 603
BsaJI CCNNGG 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 2 cut(s) 390, 517
Bse1I ACTGG 1 cut(s) 83
Bse3DI GCAATG 1 cut(s) 288
Bse8I GATNNNNATC 1 cut(s) 603
BseDI CCNNGG 1 cut(s) 511
BseGI GGATG 2 cut(s) 40, 592
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 2 cut(s) 390, 517
BseMI GCAATG 1 cut(s) 288
BseNI ACTGG 1 cut(s) 83
BseSI GKGCMC 3 cut(s) 86, 402, 547
BseXI GCAGC 6 cut(s) 130, 152, 427, 493, 574, 622
BseYI CCCAGC 1 cut(s) 232
BsgI GTGCAG 1 cut(s) 573
BshFI GGCC 1 cut(s) 545
BsiHKAI GWGCWC 2 cut(s) 242, 402
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 2 cut(s) 426, 465
BslI CCNNNNNNNGG 2 cut(s) 390, 517
BsmAI GTCTC 2 cut(s) 28, 272
BsmBI CGTCTC 2 cut(s) 28, 272
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 1 cut(s) 545
BsoBI CYCGRG 1 cut(s) 309
Bsp119I TTCGAA 1 cut(s) 166
Bsp120I GGGCCC 1 cut(s) 543
Bsp1286I GDGCHC 5 cut(s) 86, 242, 317, 402, 547
Bsp143I GATC 4 cut(s) 37, 428, 467, 604
BspACI CCGC 2 cut(s) 518, 584
BspANI GGCC 1 cut(s) 545
BspHI TCATGA 1 cut(s) 270
BspLI GGNNCC 2 cut(s) 424, 545
BspPI GGATC 3 cut(s) 45, 436, 475
BspT104I TTCGAA 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 288
BsrI ACTGG 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 511
BssMI GATC 4 cut(s) 37, 428, 467, 604
BssNAI GTATAC 1 cut(s) 368
BssT1I CCWWGG 1 cut(s) 511
Bst1107I GTATAC 1 cut(s) 368
Bst4CI ACNGT 3 cut(s) 73, 94, 125
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 4 cut(s) 112, 242, 398, 483
BstF5I GGATG 2 cut(s) 40, 592
BstH2I RGCGCY 1 cut(s) 636
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 4 cut(s) 40, 431, 470, 607
BstMAI GTCTC 2 cut(s) 28, 272
BstMBI GATC 4 cut(s) 37, 428, 467, 604
BstMWI GCNNNNNNNGC 6 cut(s) 250, 431, 440, 449, 482, 515
BstSCI CCNGG 1 cut(s) 463
BstSLI GKGCMC 3 cut(s) 86, 402, 547
BstV1I GCAGC 6 cut(s) 130, 152, 427, 493, 574, 622
BstV2I GAAGAC 1 cut(s) 21
BstXI CCANNNNNNTGG 1 cut(s) 540
BstZ17I GTATAC 1 cut(s) 368
BsuRI GGCC 1 cut(s) 545
BtsCI GGATG 2 cut(s) 40, 592
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 435
Cac8I GCNNGC 4 cut(s) 112, 242, 398, 483
CaiI CAGNNNCTG 3 cut(s) 71, 376, 437
CciI TCATGA 1 cut(s) 270
CfoI GCGC 1 cut(s) 635
Cfr13I GGNCC 2 cut(s) 543, 544
CseI GACGC 1 cut(s) 323
CviAII CATG 1 cut(s) 271
DpnI GATC 4 cut(s) 39, 430, 469, 606
DpnII GATC 4 cut(s) 37, 428, 467, 604
Eco130I CCWWGG 1 cut(s) 511
Eco24I GRGCYC 2 cut(s) 317, 547
Eco32I GATATC 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 309
EcoRV GATATC 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 511
EcoT38I GRGCYC 2 cut(s) 317, 547
ErhI CCWWGG 1 cut(s) 511
Esp3I CGTCTC 2 cut(s) 28, 272
FaeI CATG 1 cut(s) 274
FaiI YATR 3 cut(s) 191, 272, 368
FatI CATG 1 cut(s) 270
FblI GTMKAC 1 cut(s) 367
Fnu4HI GCNGC 8 cut(s) 141, 144, 441, 507, 519, 585, 588, 636
FokI GGATG 2 cut(s) 27, 579
FriOI GRGCYC 2 cut(s) 317, 547
Fsp4HI GCNGC 8 cut(s) 141, 144, 441, 507, 519, 585, 588, 636
FspBI CTAG 1 cut(s) 512
GlaI GCGC 1 cut(s) 634
GluI GCNGC 8 cut(s) 141, 144, 441, 507, 519, 585, 588, 636
GsaI CCCAGC 1 cut(s) 236
HaeII RGCGCY 1 cut(s) 636
HaeIII GGCC 1 cut(s) 545
HapII CCGG 2 cut(s) 426, 465
HgaI GACGC 1 cut(s) 323
HhaI GCGC 1 cut(s) 635
Hin1II CATG 1 cut(s) 274
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 2 cut(s) 373, 599
HpaII CCGG 2 cut(s) 426, 465
Hpy166II GTNNAC 5 cut(s) 76, 257, 348, 368, 400
Hpy188I TCNGA 3 cut(s) 57, 101, 604
Hpy188III TCNNGA 4 cut(s) 11, 66, 271, 596
Hpy8I GTNNAC 5 cut(s) 76, 257, 348, 368, 400
HpyAV CCTTC 2 cut(s) 559, 569
HpyCH4III ACNGT 3 cut(s) 73, 94, 125
HpyCH4IV ACGT 3 cut(s) 30, 446, 492
HpyCH4V TGCA 7 cut(s) 110, 215, 281, 353, 400, 590, 638
HpyF10VI GCNNNNNNNGC 6 cut(s) 250, 431, 440, 449, 482, 515
HpySE526I ACGT 3 cut(s) 30, 446, 492
Hsp92II CATG 1 cut(s) 274
HspAI GCGC 1 cut(s) 633
Kzo9I GATC 4 cut(s) 37, 428, 467, 604
LmnI GCTCC 3 cut(s) 13, 237, 422
Lsp1109I GCAGC 6 cut(s) 130, 152, 427, 493, 574, 622
LweI GCATC 2 cut(s) 202, 268
MaeI CTAG 1 cut(s) 512
MaeII ACGT 3 cut(s) 30, 446, 492
MaeIII GTNAC 1 cut(s) 304
MalI GATC 4 cut(s) 39, 430, 469, 606
MboI GATC 4 cut(s) 37, 428, 467, 604
MboII GAAGA 3 cut(s) 26, 155, 665
MfeI CAATTG 1 cut(s) 639
MhlI GDGCHC 5 cut(s) 86, 242, 317, 402, 547
MluCI AATT 1 cut(s) 639
MmeI TCCRAC 1 cut(s) 400
MroXI GAANNNNTTC 1 cut(s) 293
MslI CAYNNNNRTG 2 cut(s) 101, 538
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 2 cut(s) 426, 465
MspR9I CCNGG 1 cut(s) 465
MunI CAATTG 1 cut(s) 639
Mva1269I GAATGC 1 cut(s) 586
MwoI GCNNNNNNNGC 6 cut(s) 250, 431, 440, 449, 482, 515
NciI CCSGG 1 cut(s) 465
NdeII GATC 4 cut(s) 37, 428, 467, 604
NlaIII CATG 1 cut(s) 274
NlaIV GGNNCC 2 cut(s) 424, 545
NmeAIII GCCGAG 1 cut(s) 409
NmuCI GTSAC 1 cut(s) 304
NspV TTCGAA 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 538
PagI TCATGA 1 cut(s) 270
PctI GAATGC 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 373, 599
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 8 cut(s) 142, 145, 442, 508, 520, 586, 589, 637
PspFI CCCAGC 1 cut(s) 232
PspN4I GGNNCC 2 cut(s) 424, 545
PspOMI GGGCCC 1 cut(s) 543
PspPI GGNCC 2 cut(s) 543, 544
PstNI CAGNNNCTG 3 cut(s) 71, 376, 437
PsyI GACNNNGTC 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 143
RseI CAYNNNNRTG 2 cut(s) 101, 538
SatI GCNGC 8 cut(s) 141, 144, 441, 507, 519, 585, 588, 636
Sau3AI GATC 4 cut(s) 37, 428, 467, 604
Sau96I GGNCC 2 cut(s) 543, 544
ScrFI CCNGG 1 cut(s) 465
SduI GDGCHC 5 cut(s) 86, 242, 317, 402, 547
SetI ASST 9 cut(s) 10, 33, 145, 234, 296, 359, 449, 495, 558
SfaNI GCATC 2 cut(s) 202, 268
SfuI TTCGAA 1 cut(s) 166
SmiMI CAYNNNNRTG 2 cut(s) 101, 538
Sse9I AATT 1 cut(s) 639
SsiI CCGC 2 cut(s) 518, 584
SspMI CTAG 1 cut(s) 512
StyD4I CCNGG 1 cut(s) 463
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 3 cut(s) 73, 94, 125
TaiI ACGT 3 cut(s) 33, 449, 495
TaqI TCGA 3 cut(s) 166, 182, 317
TasI AATT 1 cut(s) 639
TauI GCSGC 2 cut(s) 521, 587
TfiI GAWTC 2 cut(s) 373, 599
TscAI CASTG 1 cut(s) 442
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 6 cut(s) 140, 143, 440, 506, 587, 635
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 286, 588
TspRI CASTG 1 cut(s) 442
Tth111I GACNNNGTC 1 cut(s) 20
VneI GTGCAC 1 cut(s) 398
XmaJI CCTAGG 1 cut(s) 511
XmiI GTMKAC 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.