pycom15g08340

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008052
Physical Location & Seq
Reverse (-)
5229904 .. 5233154
3251 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1281 bp
ATGGAGGCAGTGGAGGTGGAGATCGGGGGCGATGGGGTCATACTGACGGAGGAGCCGTCGAGGGTGATCAAACACAGAGGCGGCAAGAAAAACGCGGGGAAAAAGAAAAGGAGCAAAAATAAGAGGACGATGACGACGAGTGGGAAAAAAGAAACGTTTTTTCACATGCCGAAATTTCGGTGCTTCAGCTCGAAACCCGAAACGGATACGAAAGAGGGTTTCGATATGGAGGTGGAGGATGCAACGGGTGGAAAAATAAGCCCGACCCATTTGATTATTTTGGTGAACGGTCTCATAGGCAGTGCTGAGAATTGGAAATATGCAGCGAAGCAGTTTCTGAAGAGATACCCGGAAGATGTTATTGCTCACTGTAGTGAATGCAATTACTCAATGTTGACCTTTGATGGTGTTGATGTAATGGGAGAGAGATTAGCAGAAGAGGTTATTTCTGTGATAAAACGTCACCCTAGTGTTCAGAAGATCTCGTTTGTTGGTCATTCGTTAGGCGGGCTTATTGCAAGGTATGCCATTGGAAGGCTTTATGAACGAGACATCACTGAAGAACTATCTCAAGAAAATGGAGAATATAGGAGAAATGGAGTTGAGGATCCTTTGCTGGAACACAAAGTCGAAGGCAAAATTGCTGGACTGGAGCCTGTGAATTTTATAACGTCTGCCACTCCGCACCTTGGTTCCAGAGGGCATAATCAGGTTCCACTGTTTTGTGGCGTAAAACCCCTTGAAAAAGTAGCAGCTCGTACTTCATGGTGTCTAGGTAGAAGTGGAAAACATCTGTTTTTAACTGATAAGAAGGATGGAAAACCTCCCCTTCTTCTTCAGATGGTCAATGATTCAGAAGATCTTAAATTCATATCTGCACTGCAGTCTTTCAAGCGCCGTGTTGCCTATGCAAATGTTCGTTTTGACTCCCTTGTCGGATGGAGTACATCATCTCTACGGCGTAAGAACGAGCTGCCAAAGCTTAAACATTTATCTAGAGAAGAAAACTATCCACATATTATAAATGTGGAGACAATTCAAAGTCCAAGTCCCCGAGAAGAATTACCGTTGGAAGCCATAGCCAGTGGGCGCAAGAAAATTGACTTCGAAGAGGAAATGATCAGAAACTTGACCAAAATGAGCTGGGAACGGGTGGATGTGAACTTCAGAGGAAGTAAACAAAGATACCTTGCGCACGGTACCATTCAGGTGCAAAACGATTGTCTATACTCTGATGGGGCTGATGTGATCCAACACATGGTCGACAATTTTCTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

427

Amino Acids

48.11

Weight (kDa)

8.8

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 87 - 316 2.6e-65 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 668, 1022
AasI GACNNNNNNGTC 1 cut(s) 932
Acc16I TGCGCA 1 cut(s) 1194
Acc65I GGTACC 1 cut(s) 1199
AccB1I GGYRCC 1 cut(s) 1199
AccB7I CCANNNNNTGG 1 cut(s) 1258
AccI GTMKAC 1 cut(s) 1263
AccII CGCG 1 cut(s) 95
AciI CCGC 4 cut(s) 81, 95, 507, 683
AclI AACGTT 1 cut(s) 155
AclWI GGATC 3 cut(s) 602, 615, 1243
AcsI RAATTY 3 cut(s) 173, 661, 866
AcuI CTGAAG 5 cut(s) 169, 359, 579, 821, 1150
AfaI GTAC 3 cut(s) 760, 946, 1201
AfiI CCNNNNNNNGG 3 cut(s) 534, 689, 1258
AgsI TTSAA 3 cut(s) 743, 892, 1040
AleI CACNNNNGTG 2 cut(s) 372, 468
AluBI AGCT 5 cut(s) 189, 755, 973, 982, 1143
AluI AGCT 5 cut(s) 189, 755, 973, 982, 1143
Alw26I GTCTC 3 cut(s) 296, 543, 1025
AlwI GGATC 3 cut(s) 602, 615, 1243
AlwNI CAGNNNCTG 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 1053
ApeKI GCWGC 3 cut(s) 323, 752, 973
ApoI RAATTY 3 cut(s) 173, 661, 866
ArsI GACNNNNNNTTYG 4 cut(s) 1033, 1065, 1207, 1239
Asp718I GGTACC 1 cut(s) 1199
AspLEI GCGC 3 cut(s) 897, 1092, 1195
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 3 cut(s) 76, 295, 455
AsuII TTCGAA 1 cut(s) 1107
AvaI CYCGRG 1 cut(s) 1053
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 1199
BbvI GCAGC 3 cut(s) 335, 764, 960
BccI CCATC 6 cut(s) 26, 398, 809, 835, 933, 1229
BceAI ACGGC 3 cut(s) 40, 882, 974
BciVI GTATCC 1 cut(s) 199
BclI TGATCA 2 cut(s) 66, 1119
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 3 cut(s) 296, 543, 1025
BfaI CTAG 3 cut(s) 468, 773, 996
BfmI CTRYAG 2 cut(s) 370, 881
BfoI RGCGCY 1 cut(s) 898
BfuI GTATCC 1 cut(s) 199
BglII AGATCT 2 cut(s) 480, 859
BisI GCNGC 4 cut(s) 82, 324, 753, 974
BlsI GCNGC 4 cut(s) 83, 325, 754, 975
Bme1390I CCNGG 1 cut(s) 350
BmeT110I CYCGRG 1 cut(s) 1053
BmiI GGNNCC 6 cut(s) 54, 609, 654, 694, 714, 1201
BmrFI CCNGG 1 cut(s) 350
BmsI GCATC 1 cut(s) 229
BpmI CTGGAG 1 cut(s) 671
Bpu14I TTCGAA 1 cut(s) 1107
BpuEI CTTGAG 1 cut(s) 555
BpuMI CCSGG 1 cut(s) 350
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 688
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 3 cut(s) 534, 689, 1258
Bse1I ACTGG 2 cut(s) 654, 1083
BseDI CCNNGG 1 cut(s) 688
BseGI GGATG 4 cut(s) 244, 820, 944, 1162
BseLI CCNNNNNNNGG 3 cut(s) 534, 689, 1258
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 2 cut(s) 654, 1083
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 3 cut(s) 335, 764, 960
BseYI CCCAGC 1 cut(s) 1143
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 1 cut(s) 95
BshNI GGYRCC 1 cut(s) 1199
BsiHKCI CYCGRG 1 cut(s) 1053
BsiSI CCGG 1 cut(s) 350
BslFI GGGAC 1 cut(s) 1035
BslI CCNNNNNNNGG 3 cut(s) 534, 689, 1258
BsmAI GTCTC 3 cut(s) 296, 543, 1025
BsmFI GGGAC 1 cut(s) 1035
BsmI GAATGC 1 cut(s) 383
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 1 cut(s) 1053
Bsp119I TTCGAA 1 cut(s) 1107
Bsp143I GATC 7 cut(s) 21, 66, 480, 607, 859, 1119, 1248
BspACI CCGC 4 cut(s) 81, 95, 507, 683
BspCNI CTCAG 1 cut(s) 298
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 6 cut(s) 54, 609, 654, 694, 714, 1201
BspMAI CTGCAG 1 cut(s) 885
BspPI GGATC 3 cut(s) 602, 615, 1243
BspT104I TTCGAA 1 cut(s) 1107
BspT107I GGYRCC 1 cut(s) 1199
BspTNI GGTCTC 1 cut(s) 296
BsrI ACTGG 2 cut(s) 654, 1083
BssECI CCNNGG 1 cut(s) 688
BssMI GATC 7 cut(s) 21, 66, 480, 607, 859, 1119, 1248
BssT1I CCWWGG 1 cut(s) 688
Bst4CI ACNGT 5 cut(s) 290, 371, 720, 1068, 1199
Bst6I CTCTTC 3 cut(s) 335, 432, 1104
BstAPI GCANNNNNTGC 1 cut(s) 524
BstBI TTCGAA 1 cut(s) 1107
BstC8I GCNNGC 1 cut(s) 509
BstDEI CTNAG 1 cut(s) 306
BstF5I GGATG 4 cut(s) 244, 820, 944, 1162
BstFNI CGCG 1 cut(s) 95
BstH2I RGCGCY 1 cut(s) 898
BstHHI GCGC 3 cut(s) 897, 1092, 1195
BstKTI GATC 7 cut(s) 24, 69, 483, 610, 862, 1122, 1251
BstMAI GTCTC 3 cut(s) 296, 543, 1025
BstMBI GATC 7 cut(s) 21, 66, 480, 607, 859, 1119, 1248
BstMWI GCNNNNNNNGC 2 cut(s) 524, 979
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 348
BstSFI CTRYAG 2 cut(s) 370, 881
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 3 cut(s) 335, 764, 960
BstX2I RGATCY 3 cut(s) 480, 607, 859
BstYI RGATCY 3 cut(s) 480, 607, 859
BsuI GTATCC 1 cut(s) 199
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 4 cut(s) 244, 820, 944, 1162
BtsI GCAGTG 3 cut(s) 15, 307, 878
BtsIMutI CAGTG 7 cut(s) 15, 307, 367, 555, 716, 878, 1090
Cac8I GCNNGC 1 cut(s) 509
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 3 cut(s) 897, 1092, 1195
Csp6I GTAC 3 cut(s) 759, 945, 1200
CviAII CATG 3 cut(s) 166, 765, 1258
CviQI GTAC 3 cut(s) 759, 945, 1200
DdeI CTNAG 1 cut(s) 306
DpnI GATC 7 cut(s) 23, 68, 482, 609, 861, 1121, 1250
DpnII GATC 7 cut(s) 21, 66, 480, 607, 859, 1119, 1248
DrdI GACNNNNNNGTC 1 cut(s) 932
DseDI GACNNNNNNGTC 1 cut(s) 932
Eam1104I CTCTTC 3 cut(s) 335, 432, 1104
EarI CTCTTC 3 cut(s) 335, 432, 1104
Eco130I CCWWGG 1 cut(s) 688
Eco31I GGTCTC 1 cut(s) 296
Eco57I CTGAAG 5 cut(s) 169, 359, 579, 821, 1150
Eco88I CYCGRG 1 cut(s) 1053
EcoT14I CCWWGG 1 cut(s) 688
ErhI CCWWGG 1 cut(s) 688
FaeI CATG 3 cut(s) 169, 768, 1261
FaqI GGGAC 1 cut(s) 1035
FatI CATG 3 cut(s) 165, 764, 1257
FauI CCCGC 2 cut(s) 88, 500
FbaI TGATCA 2 cut(s) 66, 1119
FblI GTMKAC 1 cut(s) 1263
Fnu4HI GCNGC 4 cut(s) 82, 324, 753, 974
FokI GGATG 4 cut(s) 251, 827, 951, 1169
Fsp4HI GCNGC 4 cut(s) 82, 324, 753, 974
FspBI CTAG 3 cut(s) 468, 773, 996
FspI TGCGCA 1 cut(s) 1194
GlaI GCGC 3 cut(s) 896, 1091, 1194
GluI GCNGC 4 cut(s) 82, 324, 753, 974
GsaI CCCAGC 1 cut(s) 1147
GsuI CTGGAG 1 cut(s) 671
HaeII RGCGCY 1 cut(s) 898
HapII CCGG 1 cut(s) 350
HhaI GCGC 3 cut(s) 897, 1092, 1195
Hin1II CATG 3 cut(s) 169, 768, 1261
Hin6I GCGC 3 cut(s) 895, 1090, 1193
HinP1I GCGC 3 cut(s) 895, 1090, 1193
HincII GTYRAC 2 cut(s) 396, 1264
HindII GTYRAC 2 cut(s) 396, 1264
HindIII AAGCTT 1 cut(s) 980
HinfI GANTC 2 cut(s) 851, 926
HpaII CCGG 1 cut(s) 350
HphI GGTGA 3 cut(s) 76, 295, 455
Hpy166II GTNNAC 5 cut(s) 286, 396, 1162, 1178, 1264
Hpy188I TCNGA 8 cut(s) 339, 477, 840, 856, 938, 1124, 1169, 1234
Hpy188III TCNNGA 3 cut(s) 572, 696, 996
Hpy8I GTNNAC 5 cut(s) 286, 396, 1162, 1178, 1264
Hpy99I CGWCG 2 cut(s) 61, 139
HpyAV CCTTC 4 cut(s) 528, 626, 805, 839
HpyCH4III ACNGT 5 cut(s) 290, 371, 720, 1068, 1199
HpyCH4IV ACGT 3 cut(s) 155, 460, 671
HpyCH4V TGCA 8 cut(s) 242, 323, 381, 518, 878, 883, 911, 1213
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 979
HpyF3I CTNAG 1 cut(s) 306
HpySE526I ACGT 3 cut(s) 155, 460, 671
Hsp92II CATG 3 cut(s) 169, 768, 1261
HspAI GCGC 3 cut(s) 895, 1090, 1193
KpnI GGTACC 1 cut(s) 1203
Ksp22I TGATCA 2 cut(s) 66, 1119
Kzo9I GATC 7 cut(s) 21, 66, 480, 607, 859, 1119, 1248
LmnI GCTCC 3 cut(s) 52, 111, 652
Lsp1109I GCAGC 3 cut(s) 335, 764, 960
LweI GCATC 1 cut(s) 229
MaeI CTAG 3 cut(s) 468, 773, 996
MaeII ACGT 3 cut(s) 155, 460, 671
MaeIII GTNAC 1 cut(s) 461
MalI GATC 7 cut(s) 23, 68, 482, 609, 861, 1121, 1250
MboI GATC 7 cut(s) 21, 66, 480, 607, 859, 1119, 1248
MflI RGATCY 3 cut(s) 480, 607, 859
MlyI GAGTC 1 cut(s) 920
MmeI TCCRAC 3 cut(s) 916, 1050, 1276
MseI TTAA 3 cut(s) 800, 864, 984
MslI CAYNNNNRTG 3 cut(s) 372, 468, 1208
MspI CCGG 1 cut(s) 350
MspR9I CCNGG 1 cut(s) 350
Mva1269I GAATGC 1 cut(s) 383
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 524, 979
NciI CCSGG 1 cut(s) 350
NdeII GATC 7 cut(s) 21, 66, 480, 607, 859, 1119, 1248
NlaIII CATG 3 cut(s) 169, 768, 1261
NlaIV GGNNCC 6 cut(s) 54, 609, 654, 694, 714, 1201
NmuCI GTSAC 1 cut(s) 461
NsbI TGCGCA 1 cut(s) 1194
NspI RCATGY 1 cut(s) 169
NspV TTCGAA 1 cut(s) 1107
OliI CACNNNNGTG 2 cut(s) 372, 468
PcsI WCGNNNNNNNCGW 2 cut(s) 53, 134
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 1 cut(s) 851
PflMI CCANNNNNTGG 1 cut(s) 1258
PkrI GCNGC 4 cut(s) 83, 325, 754, 975
PleI GAGTC 1 cut(s) 920
PpsI GAGTC 1 cut(s) 920
PsiI TTATAA 2 cut(s) 668, 1022
Psp1406I AACGTT 1 cut(s) 155
PspFI CCCAGC 1 cut(s) 1143
PspN4I GGNNCC 6 cut(s) 54, 609, 654, 694, 714, 1201
PstI CTGCAG 1 cut(s) 885
PstNI CAGNNNCTG 1 cut(s) 337
PsuI RGATCY 3 cut(s) 480, 607, 859
RsaI GTAC 3 cut(s) 760, 946, 1201
RsaNI GTAC 3 cut(s) 759, 945, 1200
RseI CAYNNNNRTG 3 cut(s) 372, 468, 1208
SalI GTCGAC 1 cut(s) 1262
SaqAI TTAA 3 cut(s) 800, 864, 984
SatI GCNGC 4 cut(s) 82, 324, 753, 974
Sau3AI GATC 7 cut(s) 21, 66, 480, 607, 859, 1119, 1248
SchI GAGTC 1 cut(s) 920
ScrFI CCNGG 1 cut(s) 350
SfaNI GCATC 1 cut(s) 229
SfcI CTRYAG 2 cut(s) 370, 881
SfuI TTCGAA 1 cut(s) 1107
SmiMI CAYNNNNRTG 3 cut(s) 372, 468, 1208
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
SsiI CCGC 4 cut(s) 81, 95, 507, 683
SspMI CTAG 3 cut(s) 468, 773, 996
StyD4I CCNGG 1 cut(s) 348
StyI CCWWGG 1 cut(s) 688
TaaI ACNGT 5 cut(s) 290, 371, 720, 1068, 1199
TaiI ACGT 3 cut(s) 158, 463, 674
TaqI TCGA 6 cut(s) 59, 191, 222, 630, 1107, 1263
TatI WGTACW 1 cut(s) 944
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 1 cut(s) 851
Tru1I TTAA 3 cut(s) 800, 864, 984
Tru9I TTAA 3 cut(s) 800, 864, 984
TscAI CASTG 7 cut(s) 15, 307, 374, 562, 723, 885, 1090
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 3 cut(s) 323, 752, 973
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 3 cut(s) 558, 753, 859
TspGWI ACGGA 2 cut(s) 62, 218
TspRI CASTG 7 cut(s) 15, 307, 374, 562, 723, 885, 1090
Van91I CCANNNNNTGG 1 cut(s) 1258
XapI RAATTY 3 cut(s) 173, 661, 866
XbaI TCTAGA 1 cut(s) 995
XceI RCATGY 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 1263
XspI CTAG 3 cut(s) 468, 773, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.