pycom15g08360

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002500
Physical Location & Seq
Forward (+)
5242416 .. 5248703
6288 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3150 bp
ATGGCTCGCCTCCGCCGTCCCACTCTCCTCATCGCATTCCTCTCCGCCCTGATAGCCTTAACTGCAAACCCTAGCCACCTCCTCCTCGCTTCCGTAGACGATGAGTTTACGATAACCGATTCCTCCGCCTCCGATTCCGATTCCTATCTCTTCCACCAAGACTACTCGCCGCCGGCTCCGCCACCTCCGCCGCCGCTCCCGCCGTCGGTATCGTGCATCGATGACCTCGGCGGCGTCGGCTCTCTGGATGCCACGTGTCAGATAGTCTCCGATTCGAACCTCACCAGCGATGTGTACATAACAGGGAAGGGCAGCTTTTATATCTTGCCCGGAGTGAGATTCGGCTGCACGATTCCTGGCTGCGCGATAATTATTAACATTACTGGCAACTTTAGTTTAGGCAGCAACGCGTCGCTTTTGGCTGGGGCTTTCGAGCTCACGGCGCACAATGCCAGCTTTCTCGAGGGCTCGGTGCTGAACACGACGGCGTTGGCGGGAAAGCCGCCGCCTCAGACGAGTGGGACTCCGCAGGGGATAGATGGGGCGGGAGGGGGACATGGAGGGCGGGGGGCGTCTTGTTTGGTGGACAAGAGGAAGCTTCCCGAGGACGTGTGGGGAGGGGATGCGTACTCGTGGTCATCACTGCAGAGGCCGGCTAGTTTTGGGAGCCGAGGCGGTTCGACCAGTAGGGAGGTAGATTATGGAGGTTTAGGGGGAGGGAGGGTGAGACTGCAAGTGAAGGAATTTCTGGTGGTGGAGGGGAGGGTTTTGGCTGAAGGAGGTGGCGGAGGGAATAGAGGTGGCGGTGGTTCTGGTGGCAGCATCTTCATTGAGGCTTATAAAATGACTGGTAGTGGCAGGATAAGTGCTTGTGGTGGTGATGGTTATGCTGGTGGTGGCGGTGGAAGAGTGTCAGTTGATGTCTATAGCAGGCATGATGACCCCCAAATTTGTGTGCATGGAGGAAATAGCTATGCTTGTCCAGAAAATGCAGGCGGGGCTGGGACTTTATATGATGCAGTTCCTCGAAGCCTTATTGTTAGCAATCATAACAAGTCAACAGATACAGAAACACTTCTTTTGGAGTTTCCTTATCAGCCTCTTTGGACAAATGTTTATATTCAGAATAAGGCTAGGGCCACTGTCCCATTGCTTTGGAGTCGTGTCCAGGTGCAAGGACAGATAAGCCTTTTGAGTGACGGTATGTTAAGCTTTGGACTTGAACATTATGCTTCATCAGAATTTGAGTTATTGGCTGAAGAATTGTTGATGAGCGACTCTGTAATCAAGGTGTATGGGGCCTTACGCATGACTGTAAAAATGTTCTTGATGTGGAATTCCAAGATACTCATAGATGGTGGGGGGGAAGAAGCTGTTGAGACATCCTCACTCGAGGCTAGCAACTTAGTTGTTCTCAGGGGATCATCTATGATACACTCTAATGCAAATCTGGGAGTTCATGGACAAGGTTTATTGAACTTGTCAGGACCTGGAGATGGGATTCAAGCACAACGTCTGGTTCTATCGCTATTCTACAGTATTCATGTTGGGCCTGGATCTTTTTTGCGTGGTCCTTTAGAGAATGCCACAACTGATTCTGTGACACCAAAGCTTTATTGTGAAAACGAAGATTGTCCCTATGAGCTACTTCATCCACCTGAAGATTGCAATGTGAACTCATCCCTGTCCTTTACTCTTAAGGTATGCCGAGTTGAAGATATCATTATTGAAGGCCTCATAGAAGGATCTGTTGTTCATTTTCACCGGGCAAGGACCATTGCTATCCATTCTTATGGAGAAATAAGTGCATCAGGAATGGGTTGTGCTGGTGGTATTGGCAGTGGCAACATGTTAAGCAATGGAATTGGCAGTGGTGGGGGGCATGGAGGTAAAGGTGGGGCTGCATGTTATAATGGCAGTTGTGTTGAGGGTGGAATCTCATATGGGAATGCGAAGTTGCCTTGTGAACTTGGTAGTGGAAGTGGATATGACTTTTCAGTTGGCTTAACCGCTGGCGGTGGTATTATCGTAATGGGTTCTTTAGAACATCCCTTGTCAAGTTTGTCTGTTGAAGGCTCGATGACAGCTGATGGTGAAAGTTTTGAAGGGACTTCTGTGAAGGAAAAGTTTGCCCTTGCTGATAATACAACTGGAGGCCCTGGGGGTGGGTCTGGTGGAACTATACTTTTGTTCTTGCAAACACTATACCTTGGTGAGACTGCCGTTCTTTCAAGTGTTGGGGGATATGTTAGTTCAATTGGTGGTGGTGGAGGTGGTGGTGGTGGAAGGATTCATTTTCATTGGTCAGATATTCCCACTGGAGATGTGTATCAGCCCATTGCAAGTGTGGACGGAAGCATCCTTGCAGGGGGAGGGGTGGGTGGAGACCAGGGTGGTGCTGGAGAAAATGGAACAGTGACGGGTAAAGCTTGCCCAAAAGGTCTTTATGGGACCTTTTGTGAGGCATGTCCAGTTGGCACTTATAAGAATGCTATTGGGTCAGATAGAGCACTTTGTCATCATTGTCCTGCTACTGAGCTTCCCCTTCGTGCAATTTATATATCTGTCCGAGGTGGTGTTGCAGAGGCTCCTTGTCCTTACAAATGCATTTCAGACAGATATCACATGCCACACTGTTATACAGCTCTTGAAGAATTGATTTACACATTTGGTGGGCCTTGGCTGTTTGGTCTTCTTCTGATTGGGCTCCTCATCCTGTTGGCTTTGGTGCTCAGTGTTGCACGTATGAAATTGGTTGGTGTTGATGAATTACCAGGCCCTGCTCCCACTCAACATGGCTCACAAATAGACCACTCTTTCCCTTTCCTGGAGTCATTGAATGAGGTTTTAGAAACAAACAGGGCTGAGGAGTCACAGAGCCATGTGCACCGGATGTATTTTATGGGTCCTAATACTTTTGGGGATCCTTGGCATTTGACTCACACTCCTCCAGAACAAATAAAAGAGATTGTATATGAGGGGCCATTCAATACATTTGTCGATGGGATTAATTCCATAGCCACTTATCAATGGTGGGAGGGAGCAATGTACAGCATTCTCTCTATTCTTGCATACCCCCTTGCATGGTCATGGCAGCAGTGGCGCCGAAGACTGAAACTGCAACGTCTGCGTGAATTTGTTCGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1050

Amino Acids

109.92

Weight (kDa)

5.27

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 806 - 880 6.3e-36 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 840, 1911, 2484
AccB1I GGYRCC 1 cut(s) 3102
AccBSI CCGCTC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 96
AccII CGCG 2 cut(s) 365, 410
AclWI GGATC 5 cut(s) 1429, 1564, 1753, 2918, 2931
AcsI RAATTY 5 cut(s) 743, 948, 1241, 1336, 3134
AcuI CTGAAG 3 cut(s) 795, 1278, 1680
AcvI CACGTG 1 cut(s) 255
AcyI GRCGYC 3 cut(s) 234, 572, 3103
AfaI GTAC 3 cut(s) 296, 629, 3050
AfiI CCNNNNNNNGG 6 cut(s) 205, 1496, 2165, 2368, 2827, 3084
AflII CTTAAG 1 cut(s) 1697
AflIII ACRYGT 4 cut(s) 254, 408, 609, 1848
AjiI CACGTC 1 cut(s) 610
AjnI CCWGG 8 cut(s) 355, 1167, 1489, 1552, 2158, 2388, 2773, 2826
AloI GAACNNNNNNTCC 2 cut(s) 1737, 1769
Alw21I GWGCWC 4 cut(s) 438, 2512, 2733, 2889
Alw26I GTCTC 5 cut(s) 271, 721, 1373, 2210, 2379
Alw44I GTGCAC 1 cut(s) 2885
AlwI GGATC 5 cut(s) 1429, 1564, 1753, 2918, 2931
AlwNI CAGNNNCTG 2 cut(s) 1490, 2780
Ama87I CYCGRG 3 cut(s) 461, 602, 1391
AoxI GGCC 9 cut(s) 650, 1137, 1299, 1550, 1732, 2155, 2675, 2776, 2981
ApaLI GTGCAC 1 cut(s) 2885
ApeKI GCWGC 7 cut(s) 312, 345, 360, 402, 819, 1901, 3094
ApoI RAATTY 5 cut(s) 743, 948, 1241, 1336, 3134
AseI ATTAAT 1 cut(s) 3009
Asp700I GAANNNNTTC 2 cut(s) 1074, 3138
AspLEI GCGC 3 cut(s) 365, 445, 3105
AsuC2I CCSGG 2 cut(s) 330, 1766
AsuHPI GGTGA 6 cut(s) 274, 736, 890, 1754, 2105, 2225
AsuII TTCGAA 1 cut(s) 275
AsuNHI GCTAGC 1 cut(s) 1397
AvaI CYCGRG 3 cut(s) 461, 602, 1391
AvaII GGWCC 5 cut(s) 1487, 1571, 1773, 2451, 2906
BaeGI GKGCMC 1 cut(s) 2889
BamHI GGATCC 1 cut(s) 2923
BanI GGYRCC 1 cut(s) 3102
BanII GRGCYC 3 cut(s) 438, 470, 2709
BauI CACGAG 1 cut(s) 631
BbrPI CACGTG 1 cut(s) 255
BbsI GAAGAC 2 cut(s) 2684, 3115
Bbv12I GWGCWC 4 cut(s) 438, 2512, 2733, 2889
BbvCI CCTCAGC 1 cut(s) 2865
BbvI GCAGC 7 cut(s) 324, 332, 347, 414, 831, 1888, 3106
BccI CCATC 6 cut(s) 533, 875, 1349, 1490, 2084, 2996
BceAI ACGGC 4 cut(s) 187, 456, 501, 2207
BciT130I CCWGG 8 cut(s) 357, 1169, 1491, 1554, 2160, 2390, 2775, 2828
BcnI CCSGG 2 cut(s) 330, 1766
BcoDI GTCTC 5 cut(s) 271, 721, 1373, 2210, 2379
BfaI CTAG 4 cut(s) 72, 657, 1134, 1398
BfmI CTRYAG 3 cut(s) 644, 925, 1534
BfoI RGCGCY 1 cut(s) 3106
BfrI CTTAAG 1 cut(s) 1697
Bme18I GGWCC 5 cut(s) 1487, 1571, 1773, 2451, 2906
BmeT110I CYCGRG 3 cut(s) 461, 602, 1391
BmgBI CACGTC 1 cut(s) 610
BmsI GCATC 7 cut(s) 225, 238, 613, 831, 1006, 1817, 2367
BmtI GCTAGC 1 cut(s) 1401
BpiI GAAGAC 2 cut(s) 2684, 3115
BplI GAGNNNNNCTC 4 cut(s) 508, 540, 1370, 1402
BpmI CTGGAG 6 cut(s) 1512, 2172, 2340, 2421, 2849, 2934
Bpu10I CCTNAGC 1 cut(s) 2865
Bpu14I TTCGAA 1 cut(s) 275
BpuMI CCSGG 2 cut(s) 330, 1766
Bsa29I ATCGAT 1 cut(s) 219
BsaAI YACGTR 2 cut(s) 255, 2744
BsaBI GATNNNNATC 2 cut(s) 144, 2328
BsaHI GRCGYC 3 cut(s) 234, 572, 3103
BsaI GGTCTC 1 cut(s) 2379
BsaWI WCCGGW 1 cut(s) 2889
BsaXI ACNNNNNCTCC 4 cut(s) 708, 738, 2929, 2959
Bsc4I CCNNNNNNNGG 6 cut(s) 205, 1496, 2165, 2368, 2827, 3084
Bse118I RCCGGY 2 cut(s) 172, 652
Bse1I ACTGG 6 cut(s) 388, 684, 853, 2155, 2323, 2471
Bse3DI GCAATG 6 cut(s) 1148, 1675, 1776, 1864, 2337, 3051
Bse8I GATNNNNATC 2 cut(s) 144, 2328
BseBI CCWGG 8 cut(s) 357, 1169, 1491, 1554, 2160, 2390, 2775, 2828
BseCI ATCGAT 1 cut(s) 219
BseGI GGATG 9 cut(s) 253, 628, 1382, 1651, 1679, 2047, 2358, 2712, 2898
BseJI GATNNNNATC 2 cut(s) 144, 2328
BseLI CCNNNNNNNGG 6 cut(s) 205, 1496, 2165, 2368, 2827, 3084
BseMI GCAATG 6 cut(s) 1148, 1675, 1776, 1864, 2337, 3051
BseMII CTCAG 5 cut(s) 524, 1429, 2526, 2746, 2856
BseNI ACTGG 6 cut(s) 388, 684, 853, 2155, 2323, 2471
BseRI GAGGAG 6 cut(s) 17, 71, 74, 2699, 2882, 2937
BseSI GKGCMC 1 cut(s) 2889
BseXI GCAGC 7 cut(s) 324, 332, 347, 414, 831, 1888, 3106
BseYI CCCAGC 2 cut(s) 422, 1001
BsgI GTGCAG 1 cut(s) 331
Bsh1236I CGCG 2 cut(s) 365, 410
BshFI GGCC 9 cut(s) 652, 1139, 1301, 1552, 1734, 2157, 2677, 2778, 2983
BshNI GGYRCC 1 cut(s) 3102
BshVI ATCGAT 1 cut(s) 219
BsiHKAI GWGCWC 4 cut(s) 438, 2512, 2733, 2889
BsiHKCI CYCGRG 3 cut(s) 461, 602, 1391
BsiSI CCGG 5 cut(s) 173, 330, 653, 1765, 2890
BslFI GGGAC 8 cut(s) 3, 535, 567, 1018, 1130, 1620, 2122, 2464
BslI CCNNNNNNNGG 6 cut(s) 205, 1496, 2165, 2368, 2827, 3084
BsmAI GTCTC 5 cut(s) 271, 721, 1373, 2210, 2379
BsmFI GGGAC 8 cut(s) 3, 535, 567, 1018, 1130, 1620, 2122, 2464
BsmI GAATGC 5 cut(s) 35, 1588, 1954, 2494, 3054
BsnI GGCC 9 cut(s) 652, 1139, 1301, 1552, 1734, 2157, 2677, 2778, 2983
Bso31I GGTCTC 1 cut(s) 2379
BsoBI CYCGRG 3 cut(s) 461, 602, 1391
Bsp119I TTCGAA 1 cut(s) 275
Bsp1286I GDGCHC 6 cut(s) 438, 470, 2512, 2709, 2733, 2889
Bsp1407I TGTACA 2 cut(s) 294, 3048
Bsp143I GATC 5 cut(s) 1421, 1556, 1745, 2923, 3143
BspANI GGCC 9 cut(s) 652, 1139, 1301, 1552, 1734, 2157, 2677, 2778, 2983
BspCNI CTCAG 5 cut(s) 523, 1428, 2527, 2745, 2857
BspDI ATCGAT 1 cut(s) 219
BspFNI CGCG 2 cut(s) 365, 410
BspMAI CTGCAG 1 cut(s) 648
BspOI GCTAGC 1 cut(s) 1401
BspPI GGATC 5 cut(s) 1429, 1564, 1753, 2918, 2931
BspT104I TTCGAA 1 cut(s) 275
BspT107I GGYRCC 1 cut(s) 3102
BspTI CTTAAG 1 cut(s) 1697
BspTNI GGTCTC 1 cut(s) 2379
BsrBI CCGCTC 1 cut(s) 196
BsrDI GCAATG 6 cut(s) 1148, 1675, 1776, 1864, 2337, 3051
BsrFI RCCGGY 2 cut(s) 172, 652
BsrGI TGTACA 2 cut(s) 294, 3048
BsrI ACTGG 6 cut(s) 388, 684, 853, 2155, 2323, 2471
BssAI RCCGGY 2 cut(s) 172, 652
BssMI GATC 5 cut(s) 1421, 1556, 1745, 2923, 3143
BssNI GRCGYC 3 cut(s) 234, 572, 3103
BssSI CACGAG 1 cut(s) 631
BssT1I CCWWGG 3 cut(s) 2209, 2678, 2927
Bst2BI CACGAG 1 cut(s) 631
Bst2UI CCWGG 8 cut(s) 357, 1169, 1491, 1554, 2160, 2390, 2775, 2828
Bst4CI ACNGT 6 cut(s) 1144, 1202, 1315, 1538, 2416, 2636
Bst6I CTCTTC 2 cut(s) 155, 901
BstACI GRCGYC 3 cut(s) 234, 572, 3103
BstAFI CTTAAG 1 cut(s) 1697
BstAPI GCANNNNNTGC 1 cut(s) 3127
BstAUI TGTACA 2 cut(s) 294, 3048
BstBAI YACGTR 2 cut(s) 255, 2744
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 9 cut(s) 7, 174, 454, 654, 932, 994, 1399, 2014, 2431
BstDEI CTNAG 6 cut(s) 510, 1405, 1415, 2535, 2732, 2865
BstF5I GGATG 9 cut(s) 253, 628, 1382, 1651, 1679, 2047, 2358, 2712, 2898
BstFNI CGCG 2 cut(s) 365, 410
BstH2I RGCGCY 1 cut(s) 3106
BstHHI GCGC 3 cut(s) 365, 445, 3105
BstKTI GATC 5 cut(s) 1424, 1559, 1748, 2926, 3146
BstMAI GTCTC 5 cut(s) 271, 721, 1373, 2210, 2379
BstMBI GATC 5 cut(s) 1421, 1556, 1745, 2923, 3143
BstNI CCWGG 8 cut(s) 357, 1169, 1491, 1554, 2160, 2390, 2775, 2828
BstNSI RCATGY 4 cut(s) 1852, 1908, 2469, 2629
BstSFI CTRYAG 3 cut(s) 644, 925, 1534
BstSLI GKGCMC 1 cut(s) 2889
BstUI CGCG 2 cut(s) 365, 410
BstV1I GCAGC 7 cut(s) 324, 332, 347, 414, 831, 1888, 3106
BstV2I GAAGAC 2 cut(s) 2684, 3115
BstX2I RGATCY 3 cut(s) 1556, 1745, 2923
BstXI CCANNNNNNTGG 2 cut(s) 1155, 1793
BstYI RGATCY 3 cut(s) 1556, 1745, 2923
Bsu15I ATCGAT 1 cut(s) 219
BsuRI GGCC 9 cut(s) 652, 1139, 1301, 1552, 1734, 2157, 2677, 2778, 2983
BsuTUI ATCGAT 1 cut(s) 219
BtgZI GCGATG 2 cut(s) 16, 303
BtrI CACGTC 1 cut(s) 610
BtsCI GGATG 9 cut(s) 253, 628, 1382, 1651, 1679, 2047, 2358, 2712, 2898
BtsI GCAGTG 4 cut(s) 641, 1846, 1876, 3104
BtsIMutI CAGTG 9 cut(s) 641, 1140, 1846, 1876, 2316, 2421, 2632, 2740, 3104
Cac8I GCNNGC 9 cut(s) 7, 174, 454, 654, 932, 994, 1399, 2014, 2431
CaiI CAGNNNCTG 2 cut(s) 1490, 2780
CfoI GCGC 3 cut(s) 365, 445, 3105
Cfr10I RCCGGY 2 cut(s) 172, 652
ClaI ATCGAT 1 cut(s) 219
CseI GACGC 3 cut(s) 223, 399, 561
Csp6I GTAC 3 cut(s) 295, 628, 3049
CviQI GTAC 3 cut(s) 295, 628, 3049
DdeI CTNAG 6 cut(s) 510, 1405, 1415, 2535, 2732, 2865
DinI GGCGCC 1 cut(s) 3104
DpnI GATC 5 cut(s) 1423, 1558, 1747, 2925, 3145
DpnII GATC 5 cut(s) 1421, 1556, 1745, 2923, 3143
Eam1104I CTCTTC 2 cut(s) 155, 901
EarI CTCTTC 2 cut(s) 155, 901
EciI GGCGGA 5 cut(s) 34, 115, 168, 177, 801
Ecl136II GAGCTC 1 cut(s) 436
Eco130I CCWWGG 3 cut(s) 2209, 2678, 2927
Eco147I AGGCCT 1 cut(s) 1734
Eco24I GRGCYC 3 cut(s) 438, 470, 2709
Eco31I GGTCTC 1 cut(s) 2379
Eco32I GATATC 2 cut(s) 1720, 2621
Eco47I GGWCC 5 cut(s) 1487, 1571, 1773, 2451, 2906
Eco53kI GAGCTC 1 cut(s) 436
Eco57I CTGAAG 3 cut(s) 795, 1278, 1680
Eco72I CACGTG 1 cut(s) 255
Eco88I CYCGRG 3 cut(s) 461, 602, 1391
EcoICRI GAGCTC 1 cut(s) 436
EcoO109I RGGNCCY 6 cut(s) 1299, 1487, 2156, 2451, 2777, 2906
EcoRI GAATTC 1 cut(s) 1336
EcoRII CCWGG 8 cut(s) 355, 1167, 1489, 1552, 2158, 2388, 2773, 2826
EcoRV GATATC 2 cut(s) 1720, 2621
EcoT14I CCWWGG 3 cut(s) 2209, 2678, 2927
EcoT22I ATGCAT 1 cut(s) 2609
EcoT38I GRGCYC 3 cut(s) 438, 470, 2709
EgeI GGCGCC 1 cut(s) 3104
EheI GGCGCC 1 cut(s) 3104
ErhI CCWWGG 3 cut(s) 2209, 2678, 2927
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 8 cut(s) 3, 535, 567, 1018, 1130, 1620, 2122, 2464
FauI CCCGC 5 cut(s) 207, 487, 538, 558, 989
FauNDI CATATG 1 cut(s) 1942
FblI GTMKAC 1 cut(s) 96
FokI GGATG 9 cut(s) 260, 635, 1369, 1638, 1666, 2034, 2345, 2699, 2905
FriOI GRGCYC 3 cut(s) 438, 470, 2709
FspBI CTAG 4 cut(s) 72, 657, 1134, 1398
GlaI GCGC 3 cut(s) 364, 444, 3104
GsaI CCCAGC 2 cut(s) 426, 1005
GsuI CTGGAG 6 cut(s) 1512, 2172, 2340, 2421, 2849, 2934
HaeII RGCGCY 1 cut(s) 3106
HaeIII GGCC 9 cut(s) 652, 1139, 1301, 1552, 1734, 2157, 2677, 2778, 2983
HapII CCGG 5 cut(s) 173, 330, 653, 1765, 2890
HgaI GACGC 3 cut(s) 223, 399, 561
HhaI GCGC 3 cut(s) 365, 445, 3105
Hin1I GRCGYC 3 cut(s) 234, 572, 3103
Hin6I GCGC 3 cut(s) 363, 443, 3103
HinP1I GCGC 3 cut(s) 363, 443, 3103
HincII GTYRAC 1 cut(s) 1059
HindII GTYRAC 1 cut(s) 1059
HindIII AAGCTT 4 cut(s) 596, 1210, 1610, 2427
HpaII CCGG 5 cut(s) 173, 330, 653, 1765, 2890
HphI GGTGA 6 cut(s) 274, 736, 890, 1754, 2105, 2225
Hpy166II GTNNAC 9 cut(s) 97, 108, 295, 586, 1059, 1675, 1967, 2350, 2887
Hpy188III TCNNGA 9 cut(s) 245, 461, 602, 983, 1327, 1485, 1812, 2648, 2951
Hpy8I GTNNAC 9 cut(s) 97, 108, 295, 586, 1059, 1675, 1967, 2350, 2887
Hpy99I CGWCG 4 cut(s) 208, 239, 415, 487
HpyCH4III ACNGT 6 cut(s) 1144, 1202, 1315, 1538, 2416, 2636
HpyCH4IV ACGT 5 cut(s) 254, 609, 1513, 2743, 3124
HpyF3I CTNAG 6 cut(s) 510, 1405, 1415, 2535, 2732, 2865
HpySE526I ACGT 5 cut(s) 254, 609, 1513, 2743, 3124
Hsp92I GRCGYC 3 cut(s) 234, 572, 3103
HspAI GCGC 3 cut(s) 363, 443, 3103
KasI GGCGCC 1 cut(s) 3102
KroI GCCGGC 2 cut(s) 172, 652
KroNI GCCGGC 2 cut(s) 174, 654
Kzo9I GATC 5 cut(s) 1421, 1556, 1745, 2923, 3143
LmnI GCTCC 7 cut(s) 181, 201, 666, 2593, 2712, 2788, 3041
Lsp1109I GCAGC 7 cut(s) 324, 332, 347, 414, 831, 1888, 3106
LweI GCATC 7 cut(s) 225, 238, 613, 831, 1006, 1817, 2367
MaeI CTAG 4 cut(s) 72, 657, 1134, 1398
MaeII ACGT 5 cut(s) 254, 609, 1513, 2743, 3124
MaeIII GTNAC 4 cut(s) 1196, 1600, 2416, 2871
MalI GATC 5 cut(s) 1423, 1558, 1747, 2925, 3145
MbiI CCGCTC 1 cut(s) 196
MboI GATC 5 cut(s) 1421, 1556, 1745, 2923, 3143
MfeI CAATTG 1 cut(s) 2256
MflI RGATCY 3 cut(s) 1556, 1745, 2923
MhlI GDGCHC 6 cut(s) 438, 470, 2512, 2709, 2733, 2889
MluI ACGCGT 1 cut(s) 408
Mly113I GGCGCC 1 cut(s) 3103
MlyI GAGTC 6 cut(s) 517, 1168, 1271, 2840, 2879, 2932
Mph1103I ATGCAT 1 cut(s) 2609
MroNI GCCGGC 2 cut(s) 172, 652
MroXI GAANNNNTTC 2 cut(s) 1074, 3138
MseI TTAA 7 cut(s) 59, 375, 1208, 1698, 1853, 2006, 3009
MslI CAYNNNNRTG 3 cut(s) 1440, 1791, 3088
MspA1I CMGCKG 2 cut(s) 2012, 2087
MspCI CTTAAG 1 cut(s) 1697
MspI CCGG 5 cut(s) 173, 330, 653, 1765, 2890
MunI CAATTG 1 cut(s) 2256
Mva1269I GAATGC 5 cut(s) 35, 1588, 1954, 2494, 3054
MvaI CCWGG 8 cut(s) 357, 1169, 1491, 1554, 2160, 2390, 2775, 2828
MvnI CGCG 2 cut(s) 365, 410
NaeI GCCGGC 2 cut(s) 174, 654
NarI GGCGCC 1 cut(s) 3103
NciI CCSGG 2 cut(s) 330, 1766
NdeI CATATG 1 cut(s) 1942
NdeII GATC 5 cut(s) 1421, 1556, 1745, 2923, 3143
NgoMIV GCCGGC 2 cut(s) 172, 652
NheI GCTAGC 1 cut(s) 1397
NmeAIII GCCGAG 3 cut(s) 207, 695, 1733
NmuCI GTSAC 4 cut(s) 1196, 1600, 2416, 2871
NsiI ATGCAT 1 cut(s) 2609
NspI RCATGY 4 cut(s) 1852, 1908, 2469, 2629
NspV TTCGAA 1 cut(s) 275
PaeR7I CTCGAG 2 cut(s) 461, 1391
PasI CCCWGGG 1 cut(s) 2159
PceI AGGCCT 1 cut(s) 1734
PciI ACATGT 1 cut(s) 1848
PcsI WCGNNNNNNNCGW 1 cut(s) 13
PctI GAATGC 5 cut(s) 35, 1588, 1954, 2494, 3054
PdiI GCCGGC 2 cut(s) 174, 654
PdmI GAANNNNTTC 2 cut(s) 1074, 3138
PfoI TCCNGGA 1 cut(s) 2826
PleI GAGTC 6 cut(s) 517, 1167, 1271, 2839, 2878, 2932
PluTI GGCGCC 1 cut(s) 3106
PmaCI CACGTG 1 cut(s) 255
PmlI CACGTG 1 cut(s) 255
PpsI GAGTC 6 cut(s) 517, 1167, 1271, 2839, 2878, 2932
Ppu21I YACGTR 2 cut(s) 255, 2744
PpuMI RGGWCCY 3 cut(s) 1487, 2451, 2906
PscI ACATGT 1 cut(s) 1848
PshBI ATTAAT 1 cut(s) 3009
PsiI TTATAA 3 cut(s) 840, 1911, 2484
Psp124BI GAGCTC 1 cut(s) 438
Psp5II RGGWCCY 3 cut(s) 1487, 2451, 2906
Psp6I CCWGG 8 cut(s) 355, 1167, 1489, 1552, 2158, 2388, 2773, 2826
PspCI CACGTG 1 cut(s) 255
PspFI CCCAGC 2 cut(s) 422, 1001
PspGI CCWGG 8 cut(s) 355, 1167, 1489, 1552, 2158, 2388, 2773, 2826
PspPPI RGGWCCY 3 cut(s) 1487, 2451, 2906
PspXI VCTCGAGB 1 cut(s) 1391
PstI CTGCAG 1 cut(s) 648
PstNI CAGNNNCTG 2 cut(s) 1490, 2780
PsuI RGATCY 3 cut(s) 1556, 1745, 2923
PvuII CAGCTG 1 cut(s) 2087
RsaI GTAC 3 cut(s) 296, 629, 3050
RsaNI GTAC 3 cut(s) 295, 628, 3049
RseI CAYNNNNRTG 3 cut(s) 1440, 1791, 3088
SacI GAGCTC 1 cut(s) 438
SaqAI TTAA 7 cut(s) 59, 375, 1208, 1698, 1853, 2006, 3009
Sau3AI GATC 5 cut(s) 1421, 1556, 1745, 2923, 3143
SchI GAGTC 6 cut(s) 517, 1168, 1271, 2840, 2879, 2932
SduI GDGCHC 6 cut(s) 438, 470, 2512, 2709, 2733, 2889
SfaNI GCATC 7 cut(s) 225, 238, 613, 831, 1006, 1817, 2367
SfcI CTRYAG 3 cut(s) 644, 925, 1534
SfoI GGCGCC 1 cut(s) 3104
Sfr274I CTCGAG 2 cut(s) 461, 1391
SfuI TTCGAA 1 cut(s) 275
SinI GGWCC 5 cut(s) 1487, 1571, 1773, 2451, 2906
SlaI CTCGAG 2 cut(s) 461, 1391
SmiMI CAYNNNNRTG 3 cut(s) 1440, 1791, 3088
SmlI CTYRAG 3 cut(s) 461, 1391, 1697
SmoI CTYRAG 3 cut(s) 461, 1391, 1697
SseBI AGGCCT 1 cut(s) 1734
SspDI GGCGCC 1 cut(s) 3102
SspMI CTAG 4 cut(s) 72, 657, 1134, 1398
SstI GAGCTC 1 cut(s) 438
StuI AGGCCT 1 cut(s) 1734
StyI CCWWGG 3 cut(s) 2209, 2678, 2927
TaaI ACNGT 6 cut(s) 1144, 1202, 1315, 1538, 2416, 2636
TaiI ACGT 5 cut(s) 257, 612, 1516, 2746, 3127
TatI WGTACW 2 cut(s) 294, 3048
TauI GCSGC 6 cut(s) 172, 193, 196, 234, 505, 508
Tru1I TTAA 7 cut(s) 59, 375, 1208, 1698, 1853, 2006, 3009
Tru9I TTAA 7 cut(s) 59, 375, 1208, 1698, 1853, 2006, 3009
TscAI CASTG 9 cut(s) 648, 1147, 1846, 1876, 2323, 2421, 2639, 2740, 3104
TseFI GTSAC 4 cut(s) 1196, 1600, 2416, 2871
TseI GCWGC 7 cut(s) 312, 345, 360, 402, 819, 1901, 3094
Tsp45I GTSAC 4 cut(s) 1196, 1600, 2416, 2871
TspGWI ACGGA 2 cut(s) 82, 2367
TspRI CASTG 9 cut(s) 648, 1147, 1846, 1876, 2323, 2421, 2639, 2740, 3104
Vha464I CTTAAG 1 cut(s) 1697
VneI GTGCAC 1 cut(s) 2885
VpaK11BI GGWCC 5 cut(s) 1487, 1571, 1773, 2451, 2906
VspI ATTAAT 1 cut(s) 3009
XapI RAATTY 5 cut(s) 743, 948, 1241, 1336, 3134
XceI RCATGY 4 cut(s) 1852, 1908, 2469, 2629
XcmI CCANNNNNNNNNTGG 2 cut(s) 2344, 2396
XhoI CTCGAG 2 cut(s) 461, 1391
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 2 cut(s) 1074, 3138
XspI CTAG 4 cut(s) 72, 657, 1134, 1398
Zsp2I ATGCAT 1 cut(s) 2609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.