pycom15g08730

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004018
Physical Location & Seq
Forward (+)
5606980 .. 5610343
3364 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1146 bp
ATGAAAAAAACAAACGGAGTGAGCGAGACTGTGGTGCAGAAGAAAGGGAACCAAAAAATGTCATCTTTATCGGCTACTCTGAATCTCTTAAGACCCTATCTGGCTCCATCGCCACCGTCCGTGAGGTTTTTTGGTGTGCGAAACTCTGCCTCTTCTTATTCTACTTCCCGGAGACTCGCTCTCTTTCAGCTCGGCTCTGCTCTGCCTCAATCACAACTGTTAGGTGCAAAAGCATCAGAGTTGCTAAGAGGAGAGGGAAACCCTTTAGAGGCTGCCCCAGTTGCGAATTTGGCACAGGCAGGCACCGCTATTGCCAAGGAAAATGCACTGGAGTGGGTAAAAAATGACAAAAGAAGAATGCTTCATGTTGTCTATCGTGTTGGGGACTTGGACAAGACTATAAAATTCTATACTGAATGTTTGGGGATGAAGCTGTTAAGAAAACGTGACATACCTGAGGATCGATATTCGAATGCTTTTCTTGGATATGGACCAGAAGAATCCAATTTTGTGGTTGAACTTACATACAATTACGGAGTGGACAAGTACAACATTGGGACTGGTTTTGGCCATTTTGGCATTGCAGTTGAAGATGTAACCAAAACCGTGGAACTTATAAAGGCAAAGGGAGGGAAAGTTACCAAGGAACCTGGTCCTGCTAAAGGTGGCAATACAATAGTTGCCTCTGTTGAGGATCCCGATGGCTACACGTTTGAGCTTTTGGAGAGAGGGCCTTCACCTGAGCCCTTTCGTCAAGTGATGCTTCGTGTAGGTGATCTTGACCGTGCTATTAACTTTTATAAGAAGGCTTATGGGATGGAACTTCTCCACAAGAGAGATCAACCTGAATACAAGTATACTGTTGCCATGTTGGGCTACGGTCCTGAAGATAAGAACGCAGTGCTTGAACTGACGTACAACTATGGGATCACAGATTATGACAAGGGAAATGGTTACGCGCAGATTGCAATAGGCACAGATGATGTTTACAAGACTGCAGAAGCAATCAAACTAAGTGGAGGGAAGATTATTCTTGATCCTGGGCCTTTGCCAGGTATCAACACAAAGATAACTGCTTGCCTCGACCCTGATGGCTGGAAATCGGTCTTTGTTGATAAGGCTGATTTTCTGAAGGAATTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

382

Amino Acids

41.9

Weight (kDa)

8.49

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 120 - 240 2.6e-26 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 122 - 224 1.2e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 252 - 367 2.9e-16 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 617, 801
AccB1I GGYRCC 1 cut(s) 302
AccI GTMKAC 1 cut(s) 857
AccII CGCG 1 cut(s) 959
AciI CCGC 1 cut(s) 306
AclWI GGATC 5 cut(s) 468, 689, 702, 935, 1031
AcoI YGGCCR 1 cut(s) 568
AcsI RAATTY 2 cut(s) 286, 404
AcuI CTGAAG 1 cut(s) 906
AfaI GTAC 2 cut(s) 548, 917
AfiI CCNNNNNNNGG 3 cut(s) 268, 662, 1052
AflII CTTAAG 1 cut(s) 88
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 3 cut(s) 518, 590, 908
AjnI CCWGG 3 cut(s) 649, 1039, 1051
AleI CACNNNNGTG 1 cut(s) 331
AluBI AGCT 3 cut(s) 190, 433, 718
AluI AGCT 3 cut(s) 190, 433, 718
Alw26I GTCTC 2 cut(s) 20, 166
AlwI GGATC 5 cut(s) 468, 689, 702, 935, 1031
AoxI GGCC 3 cut(s) 568, 731, 1043
ApeKI GCWGC 1 cut(s) 272
ApoI RAATTY 2 cut(s) 286, 404
AspLEI GCGC 1 cut(s) 961
AspS9I GGNCC 5 cut(s) 491, 653, 731, 881, 1043
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 2 cut(s) 729, 785
AsuII TTCGAA 1 cut(s) 470
AvaII GGWCC 3 cut(s) 491, 653, 881
AxyI CCTNAGG 1 cut(s) 456
BalI TGGCCA 1 cut(s) 570
BamHI GGATCC 1 cut(s) 694
BanI GGYRCC 1 cut(s) 302
BanII GRGCYC 1 cut(s) 747
BbvI GCAGC 1 cut(s) 259
BccI CCATC 4 cut(s) 115, 695, 811, 1085
BciT130I CCWGG 3 cut(s) 651, 1041, 1053
BcnI CCSGG 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 20, 166
BfmI CTRYAG 1 cut(s) 996
BfrI CTTAAG 1 cut(s) 88
BglI GCCNNNNNGGC 1 cut(s) 576
BisI GCNGC 1 cut(s) 273
BlsI GCNGC 1 cut(s) 274
Bme1390I CCNGG 4 cut(s) 169, 651, 1041, 1053
Bme18I GGWCC 3 cut(s) 491, 653, 881
BmgT120I GGNCC 5 cut(s) 491, 653, 731, 881, 1043
BmiI GGNNCC 5 cut(s) 50, 105, 304, 648, 696
BmrFI CCNGG 4 cut(s) 169, 651, 1041, 1053
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 2 cut(s) 242, 750
BmuI ACTGGG 1 cut(s) 272
BplI GAGNNNNNCTC 2 cut(s) 163, 195
BpmI CTGGAG 1 cut(s) 350
Bpu10I CCTNAGC 1 cut(s) 741
Bpu14I TTCGAA 1 cut(s) 470
BpuMI CCSGG 1 cut(s) 169
Bsa29I ATCGAT 1 cut(s) 463
BsaJI CCNNGG 4 cut(s) 315, 606, 642, 1040
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 662, 1052
Bse1I ACTGG 3 cut(s) 278, 333, 565
Bse21I CCTNAGG 1 cut(s) 456
Bse3DI GCAATG 1 cut(s) 579
BseBI CCWGG 3 cut(s) 651, 1041, 1053
BseCI ATCGAT 1 cut(s) 463
BseDI CCNNGG 4 cut(s) 315, 606, 642, 1040
BseGI GGATG 2 cut(s) 432, 822
BseLI CCNNNNNNNGG 3 cut(s) 268, 662, 1052
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 2 cut(s) 447, 732
BseNI ACTGG 3 cut(s) 278, 333, 565
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 1 cut(s) 259
BsgI GTGCAG 1 cut(s) 56
Bsh1236I CGCG 1 cut(s) 959
BshFI GGCC 3 cut(s) 570, 733, 1045
BshNI GGYRCC 1 cut(s) 302
BshVI ATCGAT 1 cut(s) 463
BsiSI CCGG 1 cut(s) 169
BslFI GGGAC 2 cut(s) 398, 571
BslI CCNNNNNNNGG 3 cut(s) 268, 662, 1052
BsmAI GTCTC 2 cut(s) 20, 166
BsmFI GGGAC 2 cut(s) 398, 571
BsmI GAATGC 2 cut(s) 363, 478
BsnI GGCC 3 cut(s) 570, 733, 1045
Bsp119I TTCGAA 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 747
Bsp143I GATC 6 cut(s) 460, 694, 775, 838, 927, 1036
BspACI CCGC 1 cut(s) 306
BspANI GGCC 3 cut(s) 570, 733, 1045
BspCNI CTCAG 2 cut(s) 448, 733
BspDI ATCGAT 1 cut(s) 463
BspFNI CGCG 1 cut(s) 959
BspLI GGNNCC 5 cut(s) 50, 105, 304, 648, 696
BspMAI CTGCAG 1 cut(s) 1000
BspPI GGATC 5 cut(s) 468, 689, 702, 935, 1031
BspT104I TTCGAA 1 cut(s) 470
BspT107I GGYRCC 1 cut(s) 302
BspTI CTTAAG 1 cut(s) 88
BsrDI GCAATG 1 cut(s) 579
BsrI ACTGG 3 cut(s) 278, 333, 565
BssECI CCNNGG 4 cut(s) 315, 606, 642, 1040
BssMI GATC 6 cut(s) 460, 694, 775, 838, 927, 1036
BssNAI GTATAC 1 cut(s) 858
BssT1I CCWWGG 2 cut(s) 315, 642
Bst1107I GTATAC 1 cut(s) 858
Bst2UI CCWGG 3 cut(s) 651, 1041, 1053
Bst4CI ACNGT 7 cut(s) 31, 117, 219, 607, 785, 862, 881
Bst6I CTCTTC 1 cut(s) 157
BstAFI CTTAAG 1 cut(s) 88
BstBI TTCGAA 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 301, 1078
BstDEI CTNAG 4 cut(s) 245, 456, 741, 1013
BstDSI CCRYGG 1 cut(s) 606
BstENI CCTNNNNNAGG 2 cut(s) 660, 1050
BstF5I GGATG 2 cut(s) 432, 822
BstFNI CGCG 1 cut(s) 959
BstHHI GCGC 1 cut(s) 961
BstKTI GATC 6 cut(s) 463, 697, 778, 841, 930, 1039
BstMAI GTCTC 2 cut(s) 20, 166
BstMBI GATC 6 cut(s) 460, 694, 775, 838, 927, 1036
BstMWI GCNNNNNNNGC 5 cut(s) 281, 290, 305, 576, 965
BstNI CCWGG 3 cut(s) 651, 1041, 1053
BstSCI CCNGG 4 cut(s) 167, 649, 1039, 1051
BstSFI CTRYAG 1 cut(s) 996
BstUI CGCG 1 cut(s) 959
BstV1I GCAGC 1 cut(s) 259
BstX2I RGATCY 1 cut(s) 694
BstXI CCANNNNNNTGG 2 cut(s) 511, 607
BstYI RGATCY 1 cut(s) 694
BstZ17I GTATAC 1 cut(s) 858
Bsu15I ATCGAT 1 cut(s) 463
Bsu36I CCTNAGG 1 cut(s) 456
BsuRI GGCC 3 cut(s) 570, 733, 1045
BsuTUI ATCGAT 1 cut(s) 463
BtgI CCRYGG 1 cut(s) 606
BtgZI GCGATG 1 cut(s) 93
BtsCI GGATG 2 cut(s) 432, 822
BtsI GCAGTG 1 cut(s) 906
BtsIMutI CAGTG 2 cut(s) 326, 906
Cac8I GCNNGC 2 cut(s) 301, 1078
CfoI GCGC 1 cut(s) 961
Cfr13I GGNCC 5 cut(s) 491, 653, 731, 881, 1043
ClaI ATCGAT 1 cut(s) 463
CsiI ACCWGGT 1 cut(s) 649
Csp6I GTAC 2 cut(s) 547, 916
CspCI CAANNNNNGTGG 2 cut(s) 997, 1032
CviAII CATG 2 cut(s) 365, 868
CviQI GTAC 2 cut(s) 547, 916
DdeI CTNAG 4 cut(s) 245, 456, 741, 1013
DpnI GATC 6 cut(s) 462, 696, 777, 840, 929, 1038
DpnII GATC 6 cut(s) 460, 694, 775, 838, 927, 1036
EaeI YGGCCR 1 cut(s) 568
Eam1104I CTCTTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 315, 642
Eco24I GRGCYC 1 cut(s) 747
Eco47I GGWCC 3 cut(s) 491, 653, 881
Eco57I CTGAAG 1 cut(s) 906
Eco81I CCTNAGG 1 cut(s) 456
EcoNI CCTNNNNNAGG 2 cut(s) 660, 1050
EcoO109I RGGNCCY 1 cut(s) 731
EcoRII CCWGG 3 cut(s) 649, 1039, 1051
EcoT14I CCWWGG 2 cut(s) 315, 642
EcoT38I GRGCYC 1 cut(s) 747
ErhI CCWWGG 2 cut(s) 315, 642
FaeI CATG 2 cut(s) 368, 871
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FaqI GGGAC 2 cut(s) 398, 571
FatI CATG 2 cut(s) 364, 867
FblI GTMKAC 1 cut(s) 857
Fnu4HI GCNGC 1 cut(s) 273
FokI GGATG 2 cut(s) 439, 829
FriOI GRGCYC 1 cut(s) 747
Fsp4HI GCNGC 1 cut(s) 273
GlaI GCGC 1 cut(s) 960
GluI GCNGC 1 cut(s) 273
GsuI CTGGAG 1 cut(s) 350
HaeIII GGCC 3 cut(s) 570, 733, 1045
HapII CCGG 1 cut(s) 169
HhaI GCGC 1 cut(s) 961
Hin1II CATG 2 cut(s) 368, 871
Hin6I GCGC 1 cut(s) 959
HinP1I GCGC 1 cut(s) 959
HinfI GANTC 3 cut(s) 82, 174, 500
HpaII CCGG 1 cut(s) 169
HphI GGTGA 2 cut(s) 729, 785
Hpy166II GTNNAC 3 cut(s) 541, 858, 988
Hpy188I TCNGA 3 cut(s) 81, 238, 1131
Hpy188III TCNNGA 4 cut(s) 698, 779, 884, 1034
Hpy8I GTNNAC 3 cut(s) 541, 858, 988
HpyAV CCTTC 3 cut(s) 744, 799, 1126
HpyCH4III ACNGT 7 cut(s) 31, 117, 219, 607, 785, 862, 881
HpyCH4IV ACGT 3 cut(s) 445, 710, 914
HpyCH4V TGCA 6 cut(s) 37, 227, 326, 584, 968, 998
HpyF10VI GCNNNNNNNGC 5 cut(s) 281, 290, 305, 576, 965
HpyF3I CTNAG 4 cut(s) 245, 456, 741, 1013
HpySE526I ACGT 3 cut(s) 445, 710, 914
Hsp92II CATG 2 cut(s) 368, 871
HspAI GCGC 1 cut(s) 959
Kzo9I GATC 6 cut(s) 460, 694, 775, 838, 927, 1036
LmnI GCTCC 1 cut(s) 109
Lsp1109I GCAGC 1 cut(s) 259
LweI GCATC 2 cut(s) 242, 750
MabI ACCWGGT 1 cut(s) 649
MaeII ACGT 3 cut(s) 445, 710, 914
MaeIII GTNAC 4 cut(s) 446, 595, 637, 953
MalI GATC 6 cut(s) 462, 696, 777, 840, 929, 1038
MboI GATC 6 cut(s) 460, 694, 775, 838, 927, 1036
MboII GAAGA 7 cut(s) 52, 144, 366, 509, 602, 899, 1036
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 1 cut(s) 747
MlsI TGGCCA 1 cut(s) 570
MluCI AATT 5 cut(s) 286, 404, 505, 529, 1136
MluNI TGGCCA 1 cut(s) 570
MlyI GAGTC 1 cut(s) 168
Mox20I TGGCCA 1 cut(s) 570
MscI TGGCCA 1 cut(s) 570
MseI TTAA 3 cut(s) 89, 437, 792
MslI CAYNNNNRTG 1 cut(s) 331
Msp20I TGGCCA 1 cut(s) 570
MspCI CTTAAG 1 cut(s) 88
MspI CCGG 1 cut(s) 169
MspR9I CCNGG 4 cut(s) 169, 651, 1041, 1053
Mva1269I GAATGC 2 cut(s) 363, 478
MvaI CCWGG 3 cut(s) 651, 1041, 1053
MvnI CGCG 1 cut(s) 959
MwoI GCNNNNNNNGC 5 cut(s) 281, 290, 305, 576, 965
NciI CCSGG 1 cut(s) 169
NdeII GATC 6 cut(s) 460, 694, 775, 838, 927, 1036
NlaIII CATG 2 cut(s) 368, 871
NlaIV GGNNCC 5 cut(s) 50, 105, 304, 648, 696
NmeAIII GCCGAG 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 446
NspV TTCGAA 1 cut(s) 470
OliI CACNNNNGTG 1 cut(s) 331
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PctI GAATGC 2 cut(s) 363, 478
PfeI GAWTC 2 cut(s) 82, 500
PfoI TCCNGGA 1 cut(s) 167
PkrI GCNGC 1 cut(s) 274
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
PsiI TTATAA 2 cut(s) 617, 801
Psp6I CCWGG 3 cut(s) 649, 1039, 1051
PspGI CCWGG 3 cut(s) 649, 1039, 1051
PspN4I GGNNCC 5 cut(s) 50, 105, 304, 648, 696
PspPI GGNCC 5 cut(s) 491, 653, 731, 881, 1043
PstI CTGCAG 1 cut(s) 1000
PsuI RGATCY 1 cut(s) 694
RsaI GTAC 2 cut(s) 548, 917
RsaNI GTAC 2 cut(s) 547, 916
RseI CAYNNNNRTG 1 cut(s) 331
SaqAI TTAA 3 cut(s) 89, 437, 792
SatI GCNGC 1 cut(s) 273
Sau3AI GATC 6 cut(s) 460, 694, 775, 838, 927, 1036
Sau96I GGNCC 5 cut(s) 491, 653, 731, 881, 1043
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 4 cut(s) 169, 651, 1041, 1053
SduI GDGCHC 1 cut(s) 747
SexAI ACCWGGT 1 cut(s) 649
SfaNI GCATC 2 cut(s) 242, 750
SfcI CTRYAG 1 cut(s) 996
SfuI TTCGAA 1 cut(s) 470
SinI GGWCC 3 cut(s) 491, 653, 881
SmiMI CAYNNNNRTG 1 cut(s) 331
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
Sse9I AATT 5 cut(s) 286, 404, 505, 529, 1136
SsiI CCGC 1 cut(s) 306
StyD4I CCNGG 4 cut(s) 167, 649, 1039, 1051
StyI CCWWGG 2 cut(s) 315, 642
TaaI ACNGT 7 cut(s) 31, 117, 219, 607, 785, 862, 881
TaiI ACGT 3 cut(s) 448, 713, 917
TaqI TCGA 3 cut(s) 463, 470, 1083
TaqII GACCGA 1 cut(s) 1093
TasI AATT 5 cut(s) 286, 404, 505, 529, 1136
TatI WGTACW 1 cut(s) 546
TfiI GAWTC 2 cut(s) 82, 500
Tru1I TTAA 3 cut(s) 89, 437, 792
Tru9I TTAA 3 cut(s) 89, 437, 792
TscAI CASTG 2 cut(s) 333, 906
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 1 cut(s) 272
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 3 cut(s) 17, 353, 443
TspGWI ACGGA 3 cut(s) 30, 109, 549
TspRI CASTG 2 cut(s) 333, 906
Vha464I CTTAAG 1 cut(s) 88
VpaK11BI GGWCC 3 cut(s) 491, 653, 881
XagI CCTNNNNNAGG 2 cut(s) 660, 1050
XapI RAATTY 2 cut(s) 286, 404
XmiI GTMKAC 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.