pycom15g09490

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010804
Physical Location & Seq
Reverse (-)
6200752 .. 6201899
1148 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 441 bp
ATGGAGTACTTGAAAAGGAGAGAATGTGAATATGATTTGACGACCCTCGTCGACTTTTACAAGGAAGGGGAGTTCTCTGAGACCGAGCCTTCCTTGACAGGAGTTATTGTTTTCACATCCACTCCAGACAAAGAATCGAACAAGTATTACCTCGGTCCTGCTCCATTGGAGGACATGGCAAGGCAAATTGCAACAGCTTCTGGGCCCTGCGGAAACAATAGAGACTATCTGTTTCTGTTGGAGAAGGCTATGTTCGATATAGGCCATGAAGAGGACTATGTGATAGAGCTAGCAAATGCGGTGAGGAAGGTTCTTGGAATAGCCGGAAAAGGATTTCCAAAGGAGAAGAAGTTGGCCGGGCCAACCCACCTCCCGCTCAAGTCCAACATCCCGGTCCTCCAACTGCATCCGCTGCCGGAAGCCATCGCCATGGACTCGTAA

Protein Analysis

147

Amino Acids

16.31

Weight (kDa)

4.98

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 2 - 103 9.9e-16 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31290 AT5G26220 AT5G26220
fragaria_vesca FvH4_2g36490 FvH4_3g36120
malus_domestica MD08G1124700.v1.1 MD15G1104100.v1.1
prunus_persica Prupe.1G456300_v2.0.a1
pyrus_communis pycom08g10340 pycom15g09450 pycom15g09490
rosa_chinensis RchiOBHm_Chr6g0305381
rosa_laevigata RLG00000010895
rosa_multiflora Rmu_co8139608.1_g000001 Rmu_sc0002782.1_g000008
rosa_roxburghii Rroxscaffold_7G00162860
rosa_rugosa Rorug06G0340000
rosa_samantha Rh6BG449800 Rh6CG465800
rosa_wichuraiana Rw6G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 376
AccI GTMKAC 1 cut(s) 51
AciI CCGC 4 cut(s) 210, 299, 374, 410
AcoI YGGCCR 1 cut(s) 354
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 1 cut(s) 13
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
AluBI AGCT 2 cut(s) 197, 289
AluI AGCT 2 cut(s) 197, 289
Alw26I GTCTC 2 cut(s) 74, 216
AlwNI CAGNNNCTG 1 cut(s) 200
AoxI GGCC 4 cut(s) 203, 262, 354, 359
ApaI GGGCCC 1 cut(s) 207
ApeKI GCWGC 1 cut(s) 412
AspS9I GGNCC 5 cut(s) 155, 203, 204, 359, 394
AsuC2I CCSGG 2 cut(s) 358, 392
AsuHPI GGTGA 1 cut(s) 313
AsuNHI GCTAGC 1 cut(s) 289
AvaII GGWCC 2 cut(s) 155, 394
BaeGI GKGCMC 1 cut(s) 207
BanII GRGCYC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 399
BccI CCATC 1 cut(s) 431
BcnI CCSGG 2 cut(s) 358, 392
BcoDI GTCTC 2 cut(s) 74, 216
BfaI CTAG 1 cut(s) 290
BisI GCNGC 1 cut(s) 413
BlsI GCNGC 1 cut(s) 414
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 2 cut(s) 358, 392
Bme18I GGWCC 2 cut(s) 155, 394
BmgT120I GGNCC 5 cut(s) 155, 203, 204, 359, 394
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 2 cut(s) 358, 392
BmsI GCATC 1 cut(s) 415
BmtI GCTAGC 1 cut(s) 293
BoxI GACNNNNGTC 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 2 cut(s) 358, 392
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 2 cut(s) 151, 429
BsaXI ACNNNNNCTCC 8 cut(s) 10, 40, 93, 106, 123, 136, 335, 365
Bsc4I CCNNNNNNNGG 1 cut(s) 271
BseDI CCNNGG 2 cut(s) 151, 429
BseGI GGATG 3 cut(s) 116, 387, 406
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMII CTCAG 1 cut(s) 69
BseSI GKGCMC 1 cut(s) 207
BseXI GCAGC 1 cut(s) 399
BshFI GGCC 4 cut(s) 205, 264, 356, 361
BsiSI CCGG 4 cut(s) 324, 357, 392, 416
BslI CCNNNNNNNGG 1 cut(s) 271
BsmAI GTCTC 2 cut(s) 74, 216
BsnI GGCC 4 cut(s) 205, 264, 356, 361
Bso31I GGTCTC 1 cut(s) 74
Bsp120I GGGCCC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 207
Bsp19I CCATGG 1 cut(s) 429
BspACI CCGC 4 cut(s) 210, 299, 374, 410
BspANI GGCC 4 cut(s) 205, 264, 356, 361
BspCNI CTCAG 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 205
BspOI GCTAGC 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 74
BsrBI CCGCTC 1 cut(s) 376
BssECI CCNNGG 2 cut(s) 151, 429
BssT1I CCWWGG 1 cut(s) 429
Bst6I CTCTTC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 412
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 1 cut(s) 78
BstDSI CCRYGG 1 cut(s) 429
BstF5I GGATG 3 cut(s) 116, 387, 406
BstMAI GTCTC 2 cut(s) 74, 216
BstMWI GCNNNNNNNGC 1 cut(s) 412
BstPAI GACNNNNGTC 1 cut(s) 47
BstSCI CCNGG 2 cut(s) 356, 390
BstSLI GKGCMC 1 cut(s) 207
BstV1I GCAGC 1 cut(s) 399
BstXI CCANNNNNNTGG 1 cut(s) 430
BsuRI GGCC 4 cut(s) 205, 264, 356, 361
BtgI CCRYGG 1 cut(s) 429
BtgZI GCGATG 1 cut(s) 409
BtsCI GGATG 3 cut(s) 116, 387, 406
Cac8I GCNNGC 1 cut(s) 291
CaiI CAGNNNCTG 1 cut(s) 200
Cfr13I GGNCC 5 cut(s) 155, 203, 204, 359, 394
Csp6I GTAC 1 cut(s) 7
CviAII CATG 3 cut(s) 175, 266, 430
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 1 cut(s) 78
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 74
Eco47I GGWCC 2 cut(s) 155, 394
EcoO109I RGGNCCY 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 3 cut(s) 178, 269, 433
FaiI YATR 7 cut(s) 33, 176, 251, 260, 267, 279, 431
FatI CATG 3 cut(s) 174, 265, 429
FauI CCCGC 1 cut(s) 381
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 1 cut(s) 413
FokI GGATG 3 cut(s) 103, 374, 393
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 1 cut(s) 413
FspBI CTAG 1 cut(s) 290
GluI GCNGC 1 cut(s) 413
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 4 cut(s) 205, 264, 356, 361
HapII CCGG 4 cut(s) 324, 357, 392, 416
Hin1II CATG 3 cut(s) 178, 269, 433
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 2 cut(s) 134, 434
HpaII CCGG 4 cut(s) 324, 357, 392, 416
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 79
Hpy188III TCNNGA 1 cut(s) 125
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 4 cut(s) 59, 99, 238, 301
HpyCH4V TGCA 2 cut(s) 191, 406
HpyF10VI GCNNNNNNNGC 1 cut(s) 412
HpyF3I CTNAG 1 cut(s) 78
Hsp92II CATG 3 cut(s) 178, 269, 433
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 9 cut(s) 84, 138, 171, 186, 220, 337, 370, 405, 429
Lsp1109I GCAGC 1 cut(s) 399
LweI GCATC 1 cut(s) 415
MaeI CTAG 1 cut(s) 290
MbiI CCGCTC 1 cut(s) 376
MboII GAAGA 2 cut(s) 281, 358
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 1 cut(s) 186
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 3 cut(s) 219, 408, 424
MnlI CCTC 7 cut(s) 56, 161, 163, 265, 297, 380, 407
MslI CAYNNNNRTG 1 cut(s) 428
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 4 cut(s) 324, 357, 392, 416
MspR9I CCNGG 2 cut(s) 358, 392
MwoI GCNNNNNNNGC 1 cut(s) 412
NciI CCSGG 2 cut(s) 358, 392
NcoI CCATGG 1 cut(s) 429
NheI GCTAGC 1 cut(s) 289
NlaIII CATG 3 cut(s) 178, 269, 433
NlaIV GGNNCC 1 cut(s) 205
PfeI GAWTC 1 cut(s) 134
PkrI GCNGC 1 cut(s) 414
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
PshAI GACNNNNGTC 1 cut(s) 47
PspN4I GGNNCC 1 cut(s) 205
PspOMI GGGCCC 1 cut(s) 203
PspPI GGNCC 5 cut(s) 155, 203, 204, 359, 394
PsrI GAACNNNNNNTAC 2 cut(s) 131, 163
PstNI CAGNNNCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
RseI CAYNNNNRTG 1 cut(s) 428
SalI GTCGAC 1 cut(s) 50
SatI GCNGC 1 cut(s) 413
Sau96I GGNCC 5 cut(s) 155, 203, 204, 359, 394
ScaI AGTACT 1 cut(s) 8
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 2 cut(s) 358, 392
SduI GDGCHC 1 cut(s) 207
SetI ASST 5 cut(s) 153, 199, 291, 312, 372
SfaNI GCATC 1 cut(s) 415
SinI GGWCC 2 cut(s) 155, 394
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 1 cut(s) 186
SsiI CCGC 4 cut(s) 210, 299, 374, 410
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 2 cut(s) 356, 390
StyI CCWWGG 1 cut(s) 429
TaqI TCGA 3 cut(s) 51, 137, 255
TaqII GACCGA 2 cut(s) 98, 143
TasI AATT 1 cut(s) 186
TatI WGTACW 1 cut(s) 6
TfiI GAWTC 1 cut(s) 134
TseI GCWGC 1 cut(s) 412
TspDTI ATGAA 1 cut(s) 282
VpaK11BI GGWCC 2 cut(s) 155, 394
XmiI GTMKAC 1 cut(s) 51
XspI CTAG 1 cut(s) 290
ZrmI AGTACT 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.