pycom15g13670
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009208
Physical Location & Seq
Reverse (-)
9271751 .. 9273430
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 699 bp
ATGGCGGAGCGCCACGAGAGCTCCGATGCTCTCCCAACGGAGAAACTACACCCAAGGAAAGGAGAACAAGAAGAAGGCGGCGACGGAGACGAAGACGACGACGAAGAACGAAAGCAACGACTCGGTGAATTTCAACCCGGTGAGTTAAAGAGTTCTGTATCCGAGTTGAGGCAAACGCAATTAGAAACCCTAACGGCGCCTTCGACGCAGGAATTTTCAGAGAACGATCAGTTTGCAGGTTTGCAGCTTGAGTCCGTGTCACAGTCGATTGATGGAGCTGAATTGCAGGAGCAACTTGGGCTGAGCCACCAGGAAATTTTGGCAAGTGTAAGGAACCAAGATATTCAGATTCAGAAGCAAAGTCAGTCTCAGTTGACTGTTTATCCAACTCCGTTTTCAGAACTATCTCCAACTTCTGTCACTCAATCCATTTCATCTGCTCCAAGCCCAATTCTAATAGAACAAAGAGTTCCTCATGGAAAGACATCTGCTTCAGATGGTTACAACTGGCGGAAATATGGTCAGAAGCAAGTAAAGAGCCCTCTAGGTTCTCGAAGCTATTATAGGTGCACATATTCTGAGTGTTATGCCAAGAAAATAGAATGCTGTGATCACTCAGGCCATGTAACAGATATTGTTTACAAAAGCCAACATACTCATCGATCCCCCTCGGAAAAGTATCTGCATAAAGGAGAATAA

Protein Analysis

233

Amino Acids

26.07

Weight (kDa)

5.24

Isoelectric Point (pI)

74.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 166 - 220 6.6e-18 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 196
AciI CCGC 3 cut(s) 5, 78, 511
AclWI GGATC 1 cut(s) 657
AcsI RAATTY 3 cut(s) 128, 212, 315
AcuI CTGAAG 1 cut(s) 477
AcyI GRCGYC 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 59, 168
AgsI TTSAA 1 cut(s) 134
AjnI CCWGG 1 cut(s) 309
AjuI GAANNNNNNNTTGG 2 cut(s) 379, 411
AluBI AGCT 4 cut(s) 21, 247, 278, 558
AluI AGCT 4 cut(s) 21, 247, 278, 558
Alw21I GWGCWC 2 cut(s) 23, 572
Alw26I GTCTC 2 cut(s) 81, 372
Alw44I GTGCAC 1 cut(s) 568
AlwI GGATC 1 cut(s) 657
AoxI GGCC 1 cut(s) 619
ApaLI GTGCAC 1 cut(s) 568
ApeKI GCWGC 1 cut(s) 244
ApoI RAATTY 3 cut(s) 128, 212, 315
AspLEI GCGC 2 cut(s) 12, 199
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 2 cut(s) 137, 152
BaeGI GKGCMC 1 cut(s) 572
BanI GGYRCC 1 cut(s) 196
BanII GRGCYC 2 cut(s) 23, 542
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 2 cut(s) 23, 572
BbvI GCAGC 1 cut(s) 256
BccI CCATC 2 cut(s) 266, 491
BceAI ACGGC 1 cut(s) 210
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 1 cut(s) 311
BciVI GTATCC 1 cut(s) 169
BclI TGATCA 1 cut(s) 610
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 2 cut(s) 81, 372
BfaI CTAG 1 cut(s) 545
BfoI RGCGCY 2 cut(s) 13, 200
BfuAI ACCTGC 1 cut(s) 227
BfuI GTATCC 1 cut(s) 169
BisI GCNGC 2 cut(s) 79, 245
BlpI GCTNAGC 1 cut(s) 302
BlsI GCNGC 2 cut(s) 80, 246
Bme1390I CCNGG 2 cut(s) 138, 311
BmiI GGNNCC 2 cut(s) 198, 335
BmrFI CCNGG 2 cut(s) 138, 311
BmsI GCATC 1 cut(s) 16
BpiI GAAGAC 1 cut(s) 99
Bpu1102I GCTNAGC 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 661
BsaHI GRCGYC 1 cut(s) 197
BsaJI CCNNGG 2 cut(s) 53, 669
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 168
Bse1I ACTGG 1 cut(s) 512
BseBI CCWGG 1 cut(s) 311
BseCI ATCGAT 1 cut(s) 661
BseDI CCNNGG 2 cut(s) 53, 669
BseLI CCNNNNNNNGG 2 cut(s) 59, 168
BseMII CTCAG 4 cut(s) 293, 383, 570, 630
BseNI ACTGG 1 cut(s) 512
BseSI GKGCMC 1 cut(s) 572
BseXI GCAGC 1 cut(s) 256
BshFI GGCC 1 cut(s) 621
BshNI GGYRCC 1 cut(s) 196
BshVI ATCGAT 1 cut(s) 661
BsiHKAI GWGCWC 2 cut(s) 23, 572
BsiSI CCGG 1 cut(s) 138
BslI CCNNNNNNNGG 2 cut(s) 59, 168
BsmAI GTCTC 2 cut(s) 81, 372
BsmBI CGTCTC 1 cut(s) 81
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 1 cut(s) 621
Bsp1286I GDGCHC 3 cut(s) 23, 542, 572
Bsp143I GATC 3 cut(s) 226, 610, 662
Bsp1720I GCTNAGC 1 cut(s) 302
BspACI CCGC 3 cut(s) 5, 78, 511
BspANI GGCC 1 cut(s) 621
BspCNI CTCAG 4 cut(s) 294, 382, 571, 629
BspDI ATCGAT 1 cut(s) 661
BspLI GGNNCC 2 cut(s) 198, 335
BspMI ACCTGC 1 cut(s) 227
BspPI GGATC 1 cut(s) 657
BspT107I GGYRCC 1 cut(s) 196
BsrI ACTGG 1 cut(s) 512
BssECI CCNNGG 2 cut(s) 53, 669
BssMI GATC 3 cut(s) 226, 610, 662
BssNI GRCGYC 1 cut(s) 197
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 53
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 2 cut(s) 264, 379
BstACI GRCGYC 1 cut(s) 197
BstDEI CTNAG 4 cut(s) 302, 369, 579, 616
BstH2I RGCGCY 2 cut(s) 13, 200
BstHHI GCGC 2 cut(s) 12, 199
BstKTI GATC 3 cut(s) 229, 613, 665
BstMAI GTCTC 2 cut(s) 81, 372
BstMBI GATC 3 cut(s) 226, 610, 662
BstMWI GCNNNNNNNGC 3 cut(s) 18, 205, 298
BstNI CCWGG 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 136, 309
BstSLI GKGCMC 1 cut(s) 572
BstV1I GCAGC 1 cut(s) 256
BstV2I GAAGAC 1 cut(s) 99
Bsu15I ATCGAT 1 cut(s) 661
BsuI GTATCC 1 cut(s) 169
BsuRI GGCC 1 cut(s) 621
BsuTUI ATCGAT 1 cut(s) 661
BveI ACCTGC 1 cut(s) 227
CfoI GCGC 2 cut(s) 12, 199
ClaI ATCGAT 1 cut(s) 661
CseI GACGC 1 cut(s) 214
CviAII CATG 2 cut(s) 476, 623
DdeI CTNAG 4 cut(s) 302, 369, 579, 616
DinI GGCGCC 1 cut(s) 198
DpnI GATC 3 cut(s) 228, 612, 664
DpnII GATC 3 cut(s) 226, 610, 662
EciI GGCGGA 2 cut(s) 20, 526
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 53
Eco24I GRGCYC 2 cut(s) 23, 542
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 477
EcoICRI GAGCTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 309
EcoT14I CCWWGG 1 cut(s) 53
EcoT38I GRGCYC 2 cut(s) 23, 542
EgeI GGCGCC 1 cut(s) 198
EheI GGCGCC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 53
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 2 cut(s) 479, 626
FaiI YATR 8 cut(s) 477, 519, 564, 574, 588, 624, 654, 687
FatI CATG 2 cut(s) 475, 622
FbaI TGATCA 1 cut(s) 610
Fnu4HI GCNGC 2 cut(s) 79, 245
FriOI GRGCYC 2 cut(s) 23, 542
Fsp4HI GCNGC 2 cut(s) 79, 245
FspBI CTAG 1 cut(s) 545
GlaI GCGC 2 cut(s) 11, 198
GluI GCNGC 2 cut(s) 79, 245
HaeII RGCGCY 2 cut(s) 13, 200
HaeIII GGCC 1 cut(s) 621
HapII CCGG 1 cut(s) 138
HgaI GACGC 1 cut(s) 214
HhaI GCGC 2 cut(s) 12, 199
Hin1I GRCGYC 1 cut(s) 197
Hin1II CATG 2 cut(s) 479, 626
Hin6I GCGC 2 cut(s) 10, 197
HinP1I GCGC 2 cut(s) 10, 197
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 3 cut(s) 120, 251, 349
HpaII CCGG 1 cut(s) 138
HphI GGTGA 2 cut(s) 137, 152
Hpy166II GTNNAC 3 cut(s) 375, 570, 640
Hpy188III TCNNGA 1 cut(s) 552
Hpy8I GTNNAC 3 cut(s) 375, 570, 640
Hpy99I CGWCG 4 cut(s) 86, 101, 104, 208
HpyAV CCTTC 2 cut(s) 68, 210
HpyCH4III ACNGT 2 cut(s) 264, 379
HpyCH4V TGCA 5 cut(s) 236, 244, 286, 570, 685
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 205, 298
HpyF3I CTNAG 4 cut(s) 302, 369, 579, 616
Hsp92I GRCGYC 1 cut(s) 197
Hsp92II CATG 2 cut(s) 479, 626
HspAI GCGC 2 cut(s) 10, 197
KasI GGCGCC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 610
Kzo9I GATC 3 cut(s) 226, 610, 662
LmnI GCTCC 5 cut(s) 7, 26, 275, 289, 445
LpnPI CCDG 8 cut(s) 151, 194, 222, 272, 296, 323, 493, 603
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 1 cut(s) 16
MaeI CTAG 1 cut(s) 545
MaeIII GTNAC 4 cut(s) 258, 418, 500, 625
MalI GATC 3 cut(s) 228, 612, 664
MboI GATC 3 cut(s) 226, 610, 662
MboII GAAGA 3 cut(s) 83, 104, 116
MhlI GDGCHC 3 cut(s) 23, 542, 572
MluCI AATT 6 cut(s) 128, 179, 212, 281, 315, 450
Mly113I GGCGCC 1 cut(s) 197
MlyI GAGTC 2 cut(s) 114, 260
MmeI TCCRAC 2 cut(s) 410, 434
MnlI CCTC 4 cut(s) 162, 483, 552, 679
MseI TTAA 1 cut(s) 146
MspI CCGG 1 cut(s) 138
MspR9I CCNGG 2 cut(s) 138, 311
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 3 cut(s) 18, 205, 298
NarI GGCGCC 1 cut(s) 197
NciI CCSGG 1 cut(s) 138
NdeII GATC 3 cut(s) 226, 610, 662
NlaIII CATG 2 cut(s) 479, 626
NlaIV GGNNCC 2 cut(s) 198, 335
NmuCI GTSAC 2 cut(s) 258, 418
PcsI WCGNNNNNNNCGW 4 cut(s) 21, 96, 115, 200
PctI GAATGC 1 cut(s) 608
PfeI GAWTC 1 cut(s) 349
PkrI GCNGC 2 cut(s) 80, 246
PleI GAGTC 2 cut(s) 114, 259
PluTI GGCGCC 1 cut(s) 200
PpsI GAGTC 2 cut(s) 114, 259
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 2 cut(s) 198, 335
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 2 cut(s) 79, 245
Sau3AI GATC 3 cut(s) 226, 610, 662
SchI GAGTC 2 cut(s) 114, 260
ScrFI CCNGG 2 cut(s) 138, 311
SduI GDGCHC 3 cut(s) 23, 542, 572
SetI ASST 7 cut(s) 23, 241, 249, 280, 550, 560, 569
SfaNI GCATC 1 cut(s) 16
SfoI GGCGCC 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 6 cut(s) 128, 179, 212, 281, 315, 450
SsiI CCGC 3 cut(s) 5, 78, 511
SspDI GGCGCC 1 cut(s) 196
SspMI CTAG 1 cut(s) 545
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 136, 309
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 2 cut(s) 264, 379
TaqI TCGA 4 cut(s) 203, 266, 553, 661
TasI AATT 6 cut(s) 128, 179, 212, 281, 315, 450
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 349
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TseFI GTSAC 2 cut(s) 258, 418
TseI GCWGC 1 cut(s) 244
Tsp45I GTSAC 2 cut(s) 258, 418
TspDTI ATGAA 1 cut(s) 423
TspGWI ACGGA 4 cut(s) 53, 99, 244, 381
VneI GTGCAC 1 cut(s) 568
XapI RAATTY 3 cut(s) 128, 212, 315
XspI CTAG 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.