Rorug01G0462600
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009208
Physical Location & Seq
Reverse (-)
55540479 .. 55542658
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0462600.1

Sequence Viewer

Length: 1920 bp
ATGGCTCTCTCCAAACCCACCTTCCTCACCCACCTCAAAACCCTAGCCAAACCTCCCCACCACCGCCCCACACCCCCCTCATTCCTCTCCCTCCGCTCCCTCTCCTTCGCCACCCCCGAAGACGCCGCCGCCGACCGCCGCCGCCGCAAGCGCCGCCTCCGCATCGAGCCCCCAATCTCCCCCCTCCACCGCTCCCAACAACAACAACCCCCAAAACCCCAAAACCCTAACCCTAACGCCCCCAAGCTCCCCGAACCCGTCTCCGCTCTCTCCGGCAACCGCCTCAACCTCCACAACAAAATCCTCACCCTCATCCGCCGCAACGACCTCGAAGAAGCCGCCCTCTACACCCGCCACTCCATCTACTCCAACTGCCGCCCCACCATCTACACCGTCAACTCCGTCCTCGCCGCCCAGCTCCGCCAGTCCAAGTACTCCGACCTCCTCTCCCTCCACCGCTTCATCACCCAGGCCGGCGTCGCCCCCAACATCGTCACCCACAACCTCATCTTCCAGACCTATCTCGACTGCCGGAAACCCGATACTGCTATGGAGCATTACAAGCAACTCATCAACGACGCGCCGTTCAATCCTTCTCCGACGACGTACCGGATTCTGATCAAGGGGTTGGTGGACAATGGCAAGCTGGAGAGGGCCGTGGAGCTGAAGGAGGAGATGGTTGATGTCAAGGGTTTCGAGCCGGACCCGGTTGTGTATCACTATCTGATGGTGGGGTGTGTGAGGAATTCGGATTCGGATGGGGTGTTTCGGCTTTACGAGGAGTTGAAGGAGAAGCTGGGAGGGGAGGTGGAGGATGGAGTTGTGGTTGGGAGTTTGATGAAGGGGTATTTCATCAAGGAAATGGAGAAGGAGGCAATGGAGTGTTATGAACACGCTTCGAAGGTGAGGATGAATGCGGTGGCGTATAACTCGGTGTTGGAGGCTTTGTGTGAGTATGGGAAGTTTGATGAGGCGTTGAAGTTGTTTGATAGGATGAGGGAGGAGCATTCCCCGCCGAGGAGGTTTACGGTGAATTTGGGGAGCTTTAATGTGATGGCGGACGGGTATTGTGCGCAGGGGAGGTTTAAGGAGGCGGTTGAGGTTTTCAGGAAGATGGGGGAGTATAGATGTGACCCGGATACGTTGTCGTTTAATAATTTGATTCAGCATTTGTGTAGCAATGGGATGCTGAGTGAGGCTGAGGAGGTTTATGGGGAGATGGGGGAGAAGGGAGTGAACCCGGATGAGTATACTTACTCTTTGTTGATGGATACTTGTTTTGAGGAGAATAGGCCTGATGATGCGGCTGAGTATTTTAGGAAAATGGTGGATGAGAAGTTGAGGCCGAATTTGGCTGTTTATAATAGGTTGGCTGGTGGGTTGGTTAAGGTGGGGAAGGTTGATGAGGCGAAGTCGTTTTTTGATCTTATGGGGAAGAAGCTGAAGATGGATGTTGCGAGCTATCAGTTTATAATGAAGGCATTGAGTGATGCAGGGAAATTTGATGAGGTGCTTAATGTGGTGGATACATTGTTGGAGGACGAATGGATTGAATTGAATGAGGAGTTGCAGGAGTTCATCAAAGGGGAGCTGAGAAAGGATGGGAGAGAGGATGAATTGGGAAAGCTGATAGAGGAGAAGGAAAGGCTGAAGGCTGAGGCTAAGGCGAAGGAGATTGAGGAAGCAGAAGCAGCAAAGAGAAGTAGGAGTGCTGCTGTCTCCTCGTTACTTCCGTCTAGTTTGTTTGGGAAGAAAGAGACAGGGTCTACAGAGGCCGCTGAAAAAGTAGGTGAAGCTGCCTCAGCTTCTGTGGAGGCTGCTCCAGCTGCTGACATGAACTCGGCAGAGCAAGCAATCAAAGGTGAAGGTGCAGATGAAGAATCCAAAAGCGAGGGTGCAAACGAGCAGGTAGTGGCTTGA

Protein Analysis

639

Amino Acids

71.87

Weight (kDa)

5.53

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 199 - 247 6.6e-07 PPR repeat family
PPR_1 PF12854 304 - 333 1.1e-06 PPR repeat
PPR_2 PF13041 305 - 344 6.2e-08 PPR repeat family
PPR PF01535 307 - 335 3.9e-07 PPR repeat
PPR_2 PF13041 347 - 385 3.1e-07 PPR repeat family
PPR PF01535 348 - 377 2.1e-06 PPR repeat
PPR_1 PF12854 377 - 407 1.7e-06 PPR repeat
PPR_2 PF13041 379 - 426 4e-15 PPR repeat family
PPR PF01535 383 - 412 1.9e-06 PPR repeat
PPR_3 PF13812 405 - 457 7.8e-06 Pentatricopeptide repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1362, 1472
Acc16I TGCGCA 1 cut(s) 1074
Acc36I ACCTGC 1 cut(s) 1897
AccBSI CCGCTC 3 cut(s) 96, 192, 266
AccI GTMKAC 2 cut(s) 1250, 1766
AccII CGCG 1 cut(s) 581
AcsI RAATTY 4 cut(s) 745, 1033, 1348, 1499
AcuI CTGAAG 3 cut(s) 686, 1463, 1670
AcyI GRCGYC 2 cut(s) 123, 477
AfaI GTAC 2 cut(s) 434, 608
AfiI CCNNNNNNNGG 1 cut(s) 1017
AgsI TTSAA 5 cut(s) 589, 787, 979, 1553, 1558
AjnI CCWGG 1 cut(s) 468
AjuI GAANNNNNNNTTGG 1 cut(s) 37
Alw26I GTCTC 3 cut(s) 265, 1723, 1751
AlwNI CAGNNNCTG 2 cut(s) 1808, 1829
AoxI GGCC 5 cut(s) 471, 654, 1292, 1343, 1773
ApeKI GCWGC 5 cut(s) 1691, 1712, 1796, 1817, 1826
ApoI RAATTY 4 cut(s) 745, 1033, 1348, 1499
AspLEI GCGC 3 cut(s) 153, 583, 1075
AspS9I GGNCC 2 cut(s) 654, 703
AsuC2I CCSGG 3 cut(s) 707, 1136, 1241
AsuHPI GGTGA 8 cut(s) 19, 298, 457, 487, 916, 1042, 1802, 1874
AsuII TTCGAA 1 cut(s) 899
AvaII GGWCC 1 cut(s) 703
BanII GRGCYC 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 126
BbvCI CCTCAGC 3 cut(s) 1200, 1656, 1801
BbvI GCAGC 5 cut(s) 1699, 1703, 1783, 1804, 1813
BceAI ACGGC 2 cut(s) 568, 641
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 1 cut(s) 470
BciVI GTATCC 3 cut(s) 1132, 1264, 1519
BclI TGATCA 1 cut(s) 618
BcnI CCSGG 3 cut(s) 707, 1136, 1241
BcoDI GTCTC 3 cut(s) 265, 1723, 1751
BfaI CTAG 2 cut(s) 44, 1737
BfmI CTRYAG 1 cut(s) 1767
BfoI RGCGCY 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 1897
BfuI GTATCC 3 cut(s) 1132, 1264, 1519
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 4 cut(s) 470, 707, 1136, 1241
Bme18I GGWCC 1 cut(s) 703
BmgT120I GGNCC 2 cut(s) 654, 703
BmiI GGNNCC 1 cut(s) 705
BmrFI CCNGG 4 cut(s) 470, 707, 1136, 1241
BmsI GCATC 4 cut(s) 171, 1176, 1291, 1480
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 2 cut(s) 668, 1806
Bpu10I CCTNAGC 4 cut(s) 1200, 1656, 1662, 1801
Bpu14I TTCGAA 1 cut(s) 899
BpuMI CCSGG 3 cut(s) 707, 1136, 1241
BsaBI GATNNNNATC 1 cut(s) 617
BsaHI GRCGYC 2 cut(s) 123, 477
BsaJI CCNNGG 3 cut(s) 468, 657, 1016
BsaWI WCCGGW 1 cut(s) 609
BsaXI ACNNNNNCTCC 8 cut(s) 431, 461, 792, 810, 822, 840, 932, 962
Bsc4I CCNNNNNNNGG 1 cut(s) 1017
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 1 cut(s) 424
Bse3DI GCAATG 2 cut(s) 882, 1186
Bse8I GATNNNNATC 1 cut(s) 617
BseBI CCWGG 1 cut(s) 470
BseDI CCNNGG 3 cut(s) 468, 657, 1016
BseJI GATNNNNATC 1 cut(s) 617
BseLI CCNNNNNNNGG 1 cut(s) 1017
BseMI GCAATG 2 cut(s) 882, 1186
BseMII CTCAG 6 cut(s) 1181, 1191, 1299, 1583, 1647, 1815
BseNI ACTGG 1 cut(s) 424
BseXI GCAGC 5 cut(s) 1699, 1703, 1783, 1804, 1813
BseYI CCCAGC 2 cut(s) 414, 796
BsgI GTGCAG 1 cut(s) 1890
Bsh1236I CGCG 1 cut(s) 581
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 5 cut(s) 473, 656, 1294, 1345, 1775
BsiEI CGRYCG 1 cut(s) 136
BsiSI CCGG 8 cut(s) 273, 474, 532, 610, 701, 707, 1136, 1241
BslI CCNNNNNNNGG 1 cut(s) 1017
BsmAI GTCTC 3 cut(s) 265, 1723, 1751
BsmBI CGTCTC 1 cut(s) 265
BsmI GAATGC 2 cut(s) 919, 1006
BsnI GGCC 5 cut(s) 473, 656, 1294, 1345, 1775
Bsp119I TTCGAA 1 cut(s) 899
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 2 cut(s) 618, 1423
BspANI GGCC 5 cut(s) 473, 656, 1294, 1345, 1775
BspCNI CTCAG 6 cut(s) 1182, 1192, 1300, 1584, 1648, 1814
BspFNI CGCG 1 cut(s) 581
BspLI GGNNCC 1 cut(s) 705
BspMI ACCTGC 1 cut(s) 1897
BspT104I TTCGAA 1 cut(s) 899
BsrBI CCGCTC 3 cut(s) 96, 192, 266
BsrDI GCAATG 2 cut(s) 882, 1186
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 3 cut(s) 468, 657, 1016
BssMI GATC 2 cut(s) 618, 1423
BssNAI GTATAC 1 cut(s) 1251
BssNI GRCGYC 2 cut(s) 123, 477
Bst1107I GTATAC 1 cut(s) 1251
Bst2UI CCWGG 1 cut(s) 470
Bst4CI ACNGT 2 cut(s) 394, 1030
BstACI GRCGYC 2 cut(s) 123, 477
BstBI TTCGAA 1 cut(s) 899
BstC8I GCNNGC 5 cut(s) 149, 475, 644, 1459, 1851
BstDEI CTNAG 7 cut(s) 1190, 1200, 1308, 1592, 1656, 1662, 1801
BstDSI CCRYGG 1 cut(s) 657
BstFNI CGCG 1 cut(s) 581
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 3 cut(s) 153, 583, 1075
BstKTI GATC 2 cut(s) 621, 1426
BstMAI GTCTC 3 cut(s) 265, 1723, 1751
BstMBI GATC 2 cut(s) 618, 1423
BstMCI CGRYCG 1 cut(s) 136
BstNI CCWGG 1 cut(s) 470
BstSCI CCNGG 4 cut(s) 468, 705, 1134, 1239
BstSFI CTRYAG 1 cut(s) 1767
BstUI CGCG 1 cut(s) 581
BstV1I GCAGC 5 cut(s) 1699, 1703, 1783, 1804, 1813
BstV2I GAAGAC 1 cut(s) 126
BstZ17I GTATAC 1 cut(s) 1251
BsuI GTATCC 3 cut(s) 1132, 1264, 1519
BsuRI GGCC 5 cut(s) 473, 656, 1294, 1345, 1775
BtgI CCRYGG 1 cut(s) 657
BveI ACCTGC 1 cut(s) 1897
Cac8I GCNNGC 5 cut(s) 149, 475, 644, 1459, 1851
CaiI CAGNNNCTG 2 cut(s) 1808, 1829
CfoI GCGC 3 cut(s) 153, 583, 1075
Cfr10I RCCGGY 1 cut(s) 473
Cfr13I GGNCC 2 cut(s) 654, 703
CseI GACGC 3 cut(s) 131, 466, 587
Csp6I GTAC 2 cut(s) 433, 607
CviAII CATG 1 cut(s) 1834
CviQI GTAC 2 cut(s) 433, 607
DdeI CTNAG 7 cut(s) 1190, 1200, 1308, 1592, 1656, 1662, 1801
DpnI GATC 2 cut(s) 620, 1425
DpnII GATC 2 cut(s) 618, 1423
EciI GGCGGA 3 cut(s) 305, 410, 1073
Eco147I AGGCCT 1 cut(s) 1294
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 703
Eco57I CTGAAG 3 cut(s) 686, 1463, 1670
EcoRI GAATTC 1 cut(s) 745
EcoRII CCWGG 1 cut(s) 468
EcoT38I GRGCYC 1 cut(s) 171
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 1 cut(s) 1837
FatI CATG 1 cut(s) 1833
FauI CCCGC 2 cut(s) 359, 1020
FbaI TGATCA 1 cut(s) 618
FblI GTMKAC 2 cut(s) 1250, 1766
FriOI GRGCYC 1 cut(s) 171
FspBI CTAG 2 cut(s) 44, 1737
FspI TGCGCA 1 cut(s) 1074
GlaI GCGC 3 cut(s) 152, 582, 1074
GsaI CCCAGC 2 cut(s) 418, 800
GsuI CTGGAG 2 cut(s) 668, 1806
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 5 cut(s) 473, 656, 1294, 1345, 1775
HapII CCGG 8 cut(s) 273, 474, 532, 610, 701, 707, 1136, 1241
HgaI GACGC 3 cut(s) 131, 466, 587
HhaI GCGC 3 cut(s) 153, 583, 1075
Hin1I GRCGYC 2 cut(s) 123, 477
Hin1II CATG 1 cut(s) 1837
Hin6I GCGC 3 cut(s) 151, 581, 1073
HinP1I GCGC 3 cut(s) 151, 581, 1073
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 4 cut(s) 613, 752, 1162, 1880
HpaII CCGG 8 cut(s) 273, 474, 532, 610, 701, 707, 1136, 1241
HphI GGTGA 8 cut(s) 19, 298, 457, 487, 916, 1042, 1802, 1874
Hpy166II GTNNAC 6 cut(s) 397, 634, 1026, 1237, 1251, 1767
Hpy188I TCNGA 6 cut(s) 439, 600, 618, 726, 751, 757
Hpy188III TCNNGA 3 cut(s) 514, 524, 1108
Hpy8I GTNNAC 6 cut(s) 397, 634, 1026, 1237, 1251, 1767
Hpy99I CGWCG 4 cut(s) 482, 581, 604, 607
HpyCH4III ACNGT 2 cut(s) 394, 1030
HpyCH4IV ACGT 2 cut(s) 605, 1142
HpyCH4V TGCA 4 cut(s) 1493, 1570, 1871, 1898
HpyF3I CTNAG 7 cut(s) 1190, 1200, 1308, 1592, 1656, 1662, 1801
HpySE526I ACGT 2 cut(s) 605, 1142
Hsp92I GRCGYC 2 cut(s) 123, 477
Hsp92II CATG 1 cut(s) 1837
HspAI GCGC 3 cut(s) 151, 581, 1073
KroI GCCGGC 1 cut(s) 473
KroNI GCCGGC 1 cut(s) 475
Ksp22I TGATCA 1 cut(s) 618
Kzo9I GATC 2 cut(s) 618, 1423
Lsp1109I GCAGC 5 cut(s) 1699, 1703, 1783, 1804, 1813
LweI GCATC 4 cut(s) 171, 1176, 1291, 1480
MaeI CTAG 2 cut(s) 44, 1737
MaeII ACGT 2 cut(s) 605, 1142
MaeIII GTNAC 3 cut(s) 493, 1130, 1725
MalI GATC 2 cut(s) 620, 1425
MbiI CCGCTC 3 cut(s) 96, 192, 266
MboI GATC 2 cut(s) 618, 1423
MboII GAAGA 8 cut(s) 131, 344, 502, 1123, 1447, 1456, 1762, 1889
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 7 cut(s) 745, 1033, 1156, 1348, 1499, 1553, 1616
MmeI TCCRAC 5 cut(s) 393, 462, 623, 918, 1515
MroNI GCCGGC 1 cut(s) 473
MseI TTAA 5 cut(s) 1047, 1086, 1152, 1386, 1515
MspA1I CMGCKG 2 cut(s) 1778, 1826
MspI CCGG 8 cut(s) 273, 474, 532, 610, 701, 707, 1136, 1241
MspR9I CCNGG 4 cut(s) 470, 707, 1136, 1241
Mva1269I GAATGC 2 cut(s) 919, 1006
MvaI CCWGG 1 cut(s) 470
MvnI CGCG 1 cut(s) 581
NaeI GCCGGC 1 cut(s) 475
NciI CCSGG 3 cut(s) 707, 1136, 1241
NdeII GATC 2 cut(s) 618, 1423
NgoMIV GCCGGC 1 cut(s) 473
NlaIII CATG 1 cut(s) 1837
NlaIV GGNNCC 1 cut(s) 705
NmeAIII GCCGAG 2 cut(s) 1041, 1820
NmuCI GTSAC 2 cut(s) 493, 1130
NsbI TGCGCA 1 cut(s) 1074
NspV TTCGAA 1 cut(s) 899
PceI AGGCCT 1 cut(s) 1294
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 129, 1730
PctI GAATGC 2 cut(s) 919, 1006
PdiI GCCGGC 1 cut(s) 475
PfeI GAWTC 4 cut(s) 613, 752, 1162, 1880
PflFI GACNNNGTC 1 cut(s) 1762
PsiI TTATAA 2 cut(s) 1362, 1472
Psp6I CCWGG 1 cut(s) 468
PspFI CCCAGC 2 cut(s) 414, 796
PspGI CCWGG 1 cut(s) 468
PspN4I GGNNCC 1 cut(s) 705
PspPI GGNCC 2 cut(s) 654, 703
PstNI CAGNNNCTG 2 cut(s) 1808, 1829
PsyI GACNNNGTC 1 cut(s) 1762
PvuII CAGCTG 1 cut(s) 1826
RsaI GTAC 2 cut(s) 434, 608
RsaNI GTAC 2 cut(s) 433, 607
SaqAI TTAA 5 cut(s) 1047, 1086, 1152, 1386, 1515
Sau3AI GATC 2 cut(s) 618, 1423
Sau96I GGNCC 2 cut(s) 654, 703
ScaI AGTACT 1 cut(s) 434
ScrFI CCNGG 4 cut(s) 470, 707, 1136, 1241
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 4 cut(s) 171, 1176, 1291, 1480
SfcI CTRYAG 1 cut(s) 1767
SfuI TTCGAA 1 cut(s) 899
SinI GGWCC 1 cut(s) 703
Sse9I AATT 7 cut(s) 745, 1033, 1156, 1348, 1499, 1553, 1616
SseBI AGGCCT 1 cut(s) 1294
SspMI CTAG 2 cut(s) 44, 1737
StuI AGGCCT 1 cut(s) 1294
StyD4I CCNGG 4 cut(s) 468, 705, 1134, 1239
TaaI ACNGT 2 cut(s) 394, 1030
TaiI ACGT 2 cut(s) 608, 1145
TaqI TCGA 5 cut(s) 165, 330, 525, 696, 899
TasI AATT 7 cut(s) 745, 1033, 1156, 1348, 1499, 1553, 1616
TatI WGTACW 1 cut(s) 432
TfiI GAWTC 4 cut(s) 613, 752, 1162, 1880
Tru1I TTAA 5 cut(s) 1047, 1086, 1152, 1386, 1515
Tru9I TTAA 5 cut(s) 1047, 1086, 1152, 1386, 1515
TseFI GTSAC 2 cut(s) 493, 1130
TseI GCWGC 5 cut(s) 1691, 1712, 1796, 1817, 1826
Tsp45I GTSAC 2 cut(s) 493, 1130
TspGWI ACGGA 2 cut(s) 391, 1722
Tth111I GACNNNGTC 1 cut(s) 1762
VpaK11BI GGWCC 1 cut(s) 703
XapI RAATTY 4 cut(s) 745, 1033, 1348, 1499
XmiI GTMKAC 2 cut(s) 1250, 1766
XspI CTAG 2 cut(s) 44, 1737
ZrmI AGTACT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.