Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0009208

Physical Location & Seq

Genomic Coordinates Reverse (-)

915854 .. 920098

Gene Structure 4245 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG011500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1218 bp

ATGGCCGAGCACCAGAGCTCCGATGCTCTGAAGAAAAACCGAGAGGAAGAAGAGGATGAAGAATTCGAAGAAGAAGAAGATGAAGATGAAAGTGACAAAGAAGAAGAAAACCAAAGCGGAATTCCTTCTGTGGAGTTACAAAGCTCTGGAGCGGAGTCGAGAGACGCGCGATTTGAAACCCTAGCGGTGCCTCAGTTAACTGAGAAGGATCAGTGTGCAGAGAAGGCGGTTGTTTTTTTTGGTGTTGATCTCACAGGTTTTGGGGTTAACTCTGCGACGAATGATGGAGCTCAATTTCAGGAGCCAATTGGGATAAGCCACCAGGAAGTTCTGGCAGCAGCACCGAACCAGCCTGCTCAGGGTCAGATACAAAATCTGCAACAGTTGAGTACTTATCCAACTTCTTTGTCAGAACTTTCACCAACTTCTGTTACTCAATCCATTTCACCCGCTCCAAGCCCAATTCTTCCAGAACAAAGACCGTCATTGCAGAAGGTCAATGGTTTATATACGCCTGAGGAGGACAAACAAATTTCTTCTGACTTTAAGTCTATATCGCCTGTTCCTCTTGTAAAAACATCTGCTTCAGATGGTTACAACTGGCGAAAATATGGCCAGAAGCAAGTGAAGTGTCCTCCAGGTTCGCGAAGTTATTACAGGTGCACATTTTCTGAGTGTTCTGTCAAAAAGATTGAATGCTGCGATATTTCAGGCCATATAACCGAAATTGTTTACAAAAGCCAACATACTCATGATCCCCCTCGGAGGAGTAACTGCACAAGGGAAATCAAGGTTGCATCTTCTGCTGAGTGCGTGGGCAATAGTGTTATAGAATATCCCTGCGAAATACTGAATGATTCAGAGCTGCCTATGACTTCGAAAGAACCTATACAAGAAAACCCAATTCCTGAAAGAAAACGACAGAACTCAAATGACTCTGATGGTAATGGTGATGTTATGATCAAGGAGGAGCATATCAATGAACCAGAATCCAAAAGAAGATTGAAGAAAAGTAACTCAGAATATTCAGATTCTCTCTTGAAACCTGGCAAGAAACCTAAGTTTGTAGTGCATGCAGCGGGGGATGTGGGAATATCAGGTGATGGATACAGGTGGCGCAAGTATGGACAGAAGATGGTGAAGGGAAACCCCCATCCCAGGTGCATGCCAGTTCTATCATTTGTTTTCTTTTGTTCTTTTTGTTTGACATATTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

405

Amino Acids

45.17

Weight (kDa)

5.04

Isoelectric Point (pI)

68.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	197 - 252	4.4e-18	WRKY DNA -binding domain
WRKY	PF03106	368 - 388	4.7e-07	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009208)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30935
fragaria_vesca	FvH4_1g00960 FvH4_1g00960
malus_domestica	MD02G1007900.v1.1 MD15G1152100.v1.1
prunus_persica	Prupe.7G262600_v2.0.a1
pyrus_communis	pycom02g00680 pycom02g00690 pycom15g13670
rosa_chinensis	RchiOBHm_Chr2g0085691
rosa_laevigata	RLG00000015712
rosa_multiflora	Rmu_sc0007631.1_g000005
rosa_roxburghii	Rroxscaffold_2G00155120
rosa_rugosa	Rorug01G0462300 Rorug01G0462400 Rorug01G0462500 Rorug01G0462600
rosa_samantha	Rh2AG011500 Rh2BG012000 Rh2CG012800 Rh2DG013900
rosa_wichuraiana	Rw2G001020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	187
AccBSI	CCGCTC	2 cut(s)	152, 452
AccII	CGCG	3 cut(s)	167, 169, 646
AciI	CCGC	6 cut(s)	117, 152, 185, 227, 450, 1079
AclWI	GGATC	2 cut(s)	216, 749
AcoI	YGGCCR	2 cut(s)	3, 613
AcsI	RAATTY	3 cut(s)	62, 120, 531
AcuI	CTGAAG	2 cut(s)	50, 570
AfaI	GTAC	1 cut(s)	391
AfiI	CCNNNNNNNGG	3 cut(s)	359, 765, 1158
AgsI	TTSAA	4 cut(s)	176, 695, 1006, 1042
AhdI	GACNNNNNGTC	1 cut(s)	547
AjnI	CCWGG	4 cut(s)	321, 637, 1045, 1157
AjuI	GAANNNNNNNTTGG	2 cut(s)	105, 137
AluBI	AGCT	4 cut(s)	18, 144, 290, 865
AluI	AGCT	4 cut(s)	18, 144, 290, 865
Alw21I	GWGCWC	4 cut(s)	12, 20, 292, 665
Alw26I	GTCTC	1 cut(s)	156
Alw44I	GTGCAC	1 cut(s)	661
AlwI	GGATC	2 cut(s)	216, 749
AoxI	GGCC	3 cut(s)	3, 613, 712
ApaLI	GTGCAC	1 cut(s)	661
ApeKI	GCWGC	5 cut(s)	335, 338, 699, 865, 1076
ApoI	RAATTY	3 cut(s)	62, 120, 531
ArsI	GACNNNNNNTTYG	2 cut(s)	155, 187
Asp700I	GAANNNNTTC	1 cut(s)	124
AspLEI	GCGC	2 cut(s)	169, 1119
AsuHPI	GGTGA	5 cut(s)	411, 438, 962, 1112, 1150
AsuII	TTCGAA	2 cut(s)	66, 878
AxyI	CCTNAGG	1 cut(s)	516
BaeGI	GKGCMC	1 cut(s)	665
BalI	TGGCCA	1 cut(s)	615
BanI	GGYRCC	1 cut(s)	187
BanII	GRGCYC	2 cut(s)	20, 292
Bbv12I	GWGCWC	4 cut(s)	12, 20, 292, 665
BbvI	GCAGC	5 cut(s)	347, 350, 686, 852, 1088
BccI	CCATC	6 cut(s)	278, 584, 935, 1097, 1129, 1161
BciT130I	CCWGG	4 cut(s)	323, 639, 1047, 1159
BciVI	GTATCC	1 cut(s)	1100
BclI	TGATCA	1 cut(s)	960
BcoDI	GTCTC	1 cut(s)	156
BfaI	CTAG	1 cut(s)	182
BfuI	GTATCC	1 cut(s)	1100
BisI	GCNGC	5 cut(s)	336, 339, 700, 866, 1077
BlsI	GCNGC	5 cut(s)	337, 340, 701, 867, 1078
BmcAI	AGTACT	1 cut(s)	391
Bme1390I	CCNGG	4 cut(s)	323, 639, 1047, 1159
BmeRI	GACNNNNNGTC	1 cut(s)	547
BmiI	GGNNCC	2 cut(s)	189, 303
BmrFI	CCNGG	4 cut(s)	323, 639, 1047, 1159
BmsI	GCATC	2 cut(s)	13, 806
BpmI	CTGGAG	2 cut(s)	168, 621
Bpu10I	CCTNAGC	1 cut(s)	357
Bpu14I	TTCGAA	2 cut(s)	66, 878
BsaJI	CCNNGG	2 cut(s)	761, 1157
BsaXI	ACNNNNNCTCC	1 cut(s)	32
Bsc4I	CCNNNNNNNGG	3 cut(s)	359, 765, 1158
Bse1I	ACTGG	2 cut(s)	605, 1169
Bse21I	CCTNAGG	1 cut(s)	516
Bse3DI	GCAATG	1 cut(s)	485
BseBI	CCWGG	4 cut(s)	323, 639, 1047, 1159
BseDI	CCNNGG	2 cut(s)	761, 1157
BseGI	GGATG	3 cut(s)	61, 1090, 1153
BseLI	CCNNNNNNNGG	3 cut(s)	359, 765, 1158
BseMI	GCAATG	1 cut(s)	485
BseMII	CTCAG	7 cut(s)	192, 206, 371, 507, 663, 798, 1032
BseNI	ACTGG	2 cut(s)	605, 1169
BseRI	GAGGAG	3 cut(s)	533, 781, 983
BseSI	GKGCMC	1 cut(s)	665
BseXI	GCAGC	5 cut(s)	347, 350, 686, 852, 1088
BsgI	GTGCAG	2 cut(s)	237, 760
Bsh1236I	CGCG	3 cut(s)	167, 169, 646
BshFI	GGCC	3 cut(s)	5, 615, 714
BshNI	GGYRCC	1 cut(s)	187
BsiHKAI	GWGCWC	4 cut(s)	12, 20, 292, 665
BslI	CCNNNNNNNGG	3 cut(s)	359, 765, 1158
BsmAI	GTCTC	1 cut(s)	156
BsmBI	CGTCTC	1 cut(s)	156
BsmI	GAATGC	1 cut(s)	701
BsnI	GGCC	3 cut(s)	5, 615, 714
Bsp119I	TTCGAA	2 cut(s)	66, 878
Bsp1286I	GDGCHC	4 cut(s)	12, 20, 292, 665
Bsp143I	GATC	4 cut(s)	208, 247, 754, 960
Bsp68I	TCGCGA	1 cut(s)	646
BspACI	CCGC	6 cut(s)	117, 152, 185, 227, 450, 1079
BspANI	GGCC	3 cut(s)	5, 615, 714
BspCNI	CTCAG	7 cut(s)	193, 205, 370, 508, 664, 799, 1031
BspFNI	CGCG	3 cut(s)	167, 169, 646
BspHI	TCATGA	1 cut(s)	751
BspLI	GGNNCC	2 cut(s)	189, 303
BspPI	GGATC	2 cut(s)	216, 749
BspT104I	TTCGAA	2 cut(s)	66, 878
BspT107I	GGYRCC	1 cut(s)	187
BsrBI	CCGCTC	2 cut(s)	152, 452
BsrDI	GCAATG	1 cut(s)	485
BsrI	ACTGG	2 cut(s)	605, 1169
BssECI	CCNNGG	2 cut(s)	761, 1157
BssMI	GATC	4 cut(s)	208, 247, 754, 960
Bst2UI	CCWGG	4 cut(s)	323, 639, 1047, 1159
Bst4CI	ACNGT	2 cut(s)	384, 483
Bst6I	CTCTTC	1 cut(s)	45
BstAPI	GCANNNNNTGC	1 cut(s)	803
BstBI	TTCGAA	2 cut(s)	66, 878
BstC8I	GCNNGC	3 cut(s)	354, 1074, 1166
BstDEI	CTNAG	8 cut(s)	192, 201, 357, 516, 672, 807, 1018, 1059
BstF5I	GGATG	3 cut(s)	61, 1090, 1153
BstFNI	CGCG	3 cut(s)	167, 169, 646
BstHHI	GCGC	2 cut(s)	169, 1119
BstKTI	GATC	4 cut(s)	211, 250, 757, 963
BstMAI	GTCTC	1 cut(s)	156
BstMBI	GATC	4 cut(s)	208, 247, 754, 960
BstMWI	GCNNNNNNNGC	2 cut(s)	224, 803
BstNI	CCWGG	4 cut(s)	323, 639, 1047, 1159
BstNSI	RCATGY	2 cut(s)	1076, 1168
BstSCI	CCNGG	4 cut(s)	321, 637, 1045, 1157
BstSLI	GKGCMC	1 cut(s)	665
BstUI	CGCG	3 cut(s)	167, 169, 646
BstV1I	GCAGC	5 cut(s)	347, 350, 686, 852, 1088
Bsu36I	CCTNAGG	1 cut(s)	516
BsuI	GTATCC	1 cut(s)	1100
BsuRI	GGCC	3 cut(s)	5, 615, 714
BtsCI	GGATG	3 cut(s)	61, 1090, 1153
BtsIMutI	CAGTG	1 cut(s)	218
BtuMI	TCGCGA	1 cut(s)	646
Cac8I	GCNNGC	3 cut(s)	354, 1074, 1166
CciI	TCATGA	1 cut(s)	751
CfoI	GCGC	2 cut(s)	169, 1119
CseI	GACGC	1 cut(s)	173
Csp6I	GTAC	1 cut(s)	390
CviAII	CATG	3 cut(s)	752, 1073, 1165
CviQI	GTAC	1 cut(s)	390
DdeI	CTNAG	8 cut(s)	192, 201, 357, 516, 672, 807, 1018, 1059
DpnI	GATC	4 cut(s)	210, 249, 756, 962
DpnII	GATC	4 cut(s)	208, 247, 754, 960
DriI	GACNNNNNGTC	1 cut(s)	547
EaeI	YGGCCR	2 cut(s)	3, 613
Eam1104I	CTCTTC	1 cut(s)	45
Eam1105I	GACNNNNNGTC	1 cut(s)	547
EarI	CTCTTC	1 cut(s)	45
Ecl136II	GAGCTC	2 cut(s)	18, 290
Eco24I	GRGCYC	2 cut(s)	20, 292
Eco53kI	GAGCTC	2 cut(s)	18, 290
Eco57I	CTGAAG	2 cut(s)	50, 570
Eco81I	CCTNAGG	1 cut(s)	516
EcoICRI	GAGCTC	2 cut(s)	18, 290
EcoRI	GAATTC	2 cut(s)	62, 120
EcoRII	CCWGG	4 cut(s)	321, 637, 1045, 1157
EcoT38I	GRGCYC	2 cut(s)	20, 292
Esp3I	CGTCTC	1 cut(s)	156
FaeI	CATG	3 cut(s)	755, 1076, 1168
FatI	CATG	3 cut(s)	751, 1072, 1164
FauI	CCCGC	2 cut(s)	457, 1072
FbaI	TGATCA	1 cut(s)	960
Fnu4HI	GCNGC	5 cut(s)	336, 339, 700, 866, 1077
FokI	GGATG	3 cut(s)	68, 1097, 1140
FriOI	GRGCYC	2 cut(s)	20, 292
Fsp4HI	GCNGC	5 cut(s)	336, 339, 700, 866, 1077
FspBI	CTAG	1 cut(s)	182
GlaI	GCGC	2 cut(s)	168, 1118
GluI	GCNGC	5 cut(s)	336, 339, 700, 866, 1077
GsuI	CTGGAG	2 cut(s)	168, 621
HaeIII	GGCC	3 cut(s)	5, 615, 714
HgaI	GACGC	1 cut(s)	173
HhaI	GCGC	2 cut(s)	169, 1119
Hin1II	CATG	3 cut(s)	755, 1076, 1168
Hin6I	GCGC	2 cut(s)	167, 1117
HinP1I	GCGC	2 cut(s)	167, 1117
HincII	GTYRAC	2 cut(s)	198, 268
HindII	GTYRAC	2 cut(s)	198, 268
HinfI	GANTC	5 cut(s)	155, 857, 935, 989, 1031
HpaI	GTTAAC	2 cut(s)	198, 268
HphI	GGTGA	5 cut(s)	411, 438, 962, 1112, 1150
Hpy166II	GTNNAC	4 cut(s)	198, 268, 663, 733
Hpy188III	TCNNGA	8 cut(s)	147, 159, 299, 470, 645, 752, 908, 1039
Hpy8I	GTNNAC	4 cut(s)	198, 268, 663, 733
Hpy99I	CGWCG	1 cut(s)	280
HpyAV	CCTTC	5 cut(s)	135, 199, 217, 487, 1135
HpyCH4III	ACNGT	2 cut(s)	384, 483
HpyCH4V	TGCA	9 cut(s)	218, 379, 490, 663, 777, 797, 1072, 1076, 1164
HpyF10VI	GCNNNNNNNGC	2 cut(s)	224, 803
HpyF3I	CTNAG	8 cut(s)	192, 201, 357, 516, 672, 807, 1018, 1059
Hsp92II	CATG	3 cut(s)	755, 1076, 1168
HspAI	GCGC	2 cut(s)	167, 1117
Ksp22I	TGATCA	1 cut(s)	960
KspAI	GTTAAC	2 cut(s)	198, 268
Kzo9I	GATC	4 cut(s)	208, 247, 754, 960
LmnI	GCTCC	6 cut(s)	23, 149, 287, 301, 457, 970
Lsp1109I	GCAGC	5 cut(s)	347, 350, 686, 852, 1088
LweI	GCATC	2 cut(s)	13, 806
MaeI	CTAG	1 cut(s)	182
MaeIII	GTNAC	6 cut(s)	92, 135, 430, 593, 770, 1013
MalI	GATC	4 cut(s)	210, 249, 756, 962
MbiI	CCGCTC	2 cut(s)	152, 452
MboI	GATC	4 cut(s)	208, 247, 754, 960
MfeI	CAATTG	1 cut(s)	306
MhlI	GDGCHC	4 cut(s)	12, 20, 292, 665
MlsI	TGGCCA	1 cut(s)	615
MluCI	AATT	8 cut(s)	62, 120, 293, 306, 462, 531, 726, 903
MluNI	TGGCCA	1 cut(s)	615
MlyI	GAGTC	2 cut(s)	164, 929
MmeI	TCCRAC	1 cut(s)	422
Mox20I	TGGCCA	1 cut(s)	615
MroXI	GAANNNNTTC	1 cut(s)	124
MscI	TGGCCA	1 cut(s)	615
MseI	TTAA	3 cut(s)	197, 267, 546
MslI	CAYNNNNRTG	2 cut(s)	750, 978
Msp20I	TGGCCA	1 cut(s)	615
MspA1I	CMGCKG	1 cut(s)	1079
MspR9I	CCNGG	4 cut(s)	323, 639, 1047, 1159
MunI	CAATTG	1 cut(s)	306
Mva1269I	GAATGC	1 cut(s)	701
MvaI	CCWGG	4 cut(s)	323, 639, 1047, 1159
MvnI	CGCG	3 cut(s)	167, 169, 646
MwoI	GCNNNNNNNGC	2 cut(s)	224, 803
NdeII	GATC	4 cut(s)	208, 247, 754, 960
NlaIII	CATG	3 cut(s)	755, 1076, 1168
NlaIV	GGNNCC	2 cut(s)	189, 303
NmeAIII	GCCGAG	1 cut(s)	31
NmuCI	GTSAC	1 cut(s)	92
NruI	TCGCGA	1 cut(s)	646
NspI	RCATGY	2 cut(s)	1076, 1168
NspV	TTCGAA	2 cut(s)	66, 878
PaeI	GCATGC	2 cut(s)	1076, 1168
PagI	TCATGA	1 cut(s)	751
PctI	GAATGC	1 cut(s)	701
PdmI	GAANNNNTTC	1 cut(s)	124
PfeI	GAWTC	3 cut(s)	857, 989, 1031
PkrI	GCNGC	5 cut(s)	337, 340, 701, 867, 1078
PleI	GAGTC	2 cut(s)	163, 929
PpsI	GAGTC	2 cut(s)	163, 929
Psp124BI	GAGCTC	2 cut(s)	20, 292
Psp6I	CCWGG	4 cut(s)	321, 637, 1045, 1157
PspGI	CCWGG	4 cut(s)	321, 637, 1045, 1157
PspN4I	GGNNCC	2 cut(s)	189, 303
RruI	TCGCGA	1 cut(s)	646
RsaI	GTAC	1 cut(s)	391
RsaNI	GTAC	1 cut(s)	390
RseI	CAYNNNNRTG	2 cut(s)	750, 978
SacI	GAGCTC	2 cut(s)	20, 292
SaqAI	TTAA	3 cut(s)	197, 267, 546
SatI	GCNGC	5 cut(s)	336, 339, 700, 866, 1077
Sau3AI	GATC	4 cut(s)	208, 247, 754, 960
ScaI	AGTACT	1 cut(s)	391
SchI	GAGTC	2 cut(s)	164, 929
ScrFI	CCNGG	4 cut(s)	323, 639, 1047, 1159
SduI	GDGCHC	4 cut(s)	12, 20, 292, 665
SfaNI	GCATC	2 cut(s)	13, 806
SfuI	TTCGAA	2 cut(s)	66, 878
SmiMI	CAYNNNNRTG	2 cut(s)	750, 978
SphI	GCATGC	2 cut(s)	1076, 1168
Sse9I	AATT	8 cut(s)	62, 120, 293, 306, 462, 531, 726, 903
SsiI	CCGC	6 cut(s)	117, 152, 185, 227, 450, 1079
SspI	AATATT	1 cut(s)	1025
SspMI	CTAG	1 cut(s)	182
SstI	GAGCTC	2 cut(s)	20, 292
StyD4I	CCNGG	4 cut(s)	321, 637, 1045, 1157
TaaI	ACNGT	2 cut(s)	384, 483
TaqI	TCGA	3 cut(s)	66, 158, 878
TasI	AATT	8 cut(s)	62, 120, 293, 306, 462, 531, 726, 903
TatI	WGTACW	1 cut(s)	389
TfiI	GAWTC	3 cut(s)	857, 989, 1031
Tru1I	TTAA	3 cut(s)	197, 267, 546
Tru9I	TTAA	3 cut(s)	197, 267, 546
TscAI	CASTG	1 cut(s)	218
TseFI	GTSAC	1 cut(s)	92
TseI	GCWGC	5 cut(s)	335, 338, 699, 865, 1076
Tsp45I	GTSAC	1 cut(s)	92
TspDTI	ATGAA	4 cut(s)	72, 96, 102, 996
TspRI	CASTG	1 cut(s)	218
VneI	GTGCAC	1 cut(s)	661
XapI	RAATTY	3 cut(s)	62, 120, 531
XceI	RCATGY	2 cut(s)	1076, 1168
XmnI	GAANNNNTTC	1 cut(s)	124
XspI	CTAG	1 cut(s)	182
ZrmI	AGTACT	1 cut(s)	391

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.