pycom15g14880

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006378
Physical Location & Seq
Reverse (-)
10212275 .. 10217192
4918 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3342 bp
ATGTCCGGATTTTCAGACCAGCTCGAAGAAATCAAATCCCTGATTTCTTCAGCCGCCACCAAAGCAAACAAGTCATTCGCCTACTCCACCCTCTTACACCTCCAGCAGCAATCCTCCGACTGCCACGATTCAATTCAAATGCTTGCGCGGAGCTGCCGGAGCCTCATCCGTCCGAGCGTCGCCGATGTTCGGAACAATGACGAAGAAATTGCAACTCAGGCATTGAAATGCTTGGGCTTTATGATATATCATCCGTCCATCGTCGCCGAAATCGCAGGGGTGTGGTGCATATCTATCCAACAATTGAGTACACCACTTTTAGCTGCCCATTTCCATTCTCTTTTGCTTGCGATTGTTCATGCCATTGACAATCCTATCGGTTCTTTGTCGACTACTTTTGAGGCTATTCAGGCTGTTATGAAGTTAGCAACTTTGTTAAGTGAGAATATGAGAGAGATGTCGCATATATGGGCTCCTTCAGTATATAGAAGACTCCTTAGCTCTGATAAGAGGGAGAGGGATATGTCAGAGAGGTGTTTATTGAACACTCGGTCTACAATATTACCCCCTCCGCTAAATCTTTCCAAGGCAGTTGTCAAAGATTTGAAGCCAAGGTTGCTTACTGGGATGCACCACATGCTAACCAATGGCATGAAGGTTCAAACTATTCAAGCATGGGGATGGTTTATCCGCTTACTGGGATCTCATGCCTTGAAGAACAGGCAGTTAATTAATCAGATGCTAAAAATTCCTGAGCAGACATTTTCAGATCATGATTCACAAGTTCAAATTGCTTCACAAGTTGCCTGGGAAGGTCTTATTGATGCCCTTGTTCACCCTACCGTGATATTACCCTTTGAGACAAATGCGACAAAAAAGGACAATGGTATGGAACGAATAGGAACATGCAAAGGAAACGGTAGTGAAATTCAAAAAAAGGGATACTTAAAAAGCACAAAGCTCATTATGACACCACTGATAGGCATCATGTCGACTAAATGTGATGTATCTGTTCACTTAGCTTGCTTGAACACGTGGTGTTATTTATTGCATAAACTTGATACCTCTCTCAGTGATTCTTCTATCATCAGAATGGTACTAGAGCCTGTTGTTGAGGCAGTTTTTCAAGTTGATCCTGATAGTAAGAGCATCTGGGCCAGGAATCTGTGCATAGATTTGCTTAATGTTTTCATATTGGCAAAATGTAAAGATATAGATTATGACTCGCTTAATCAAGTGAACCATCAGTTATTGGGCAAAAGCTCTAGGATTGCTCCCTTTGTATCTGGAAGTTTCTCTTGGAAGCAGTATCCCATCAAATGGTTTCCATGGGATCTGAGTCTTTTAGACTTCCATTTAAAGATGATCTACATTCTTATCTGTCAAGCGTCAAGGGAAACTGTCTCCCACGATAACAGGATTTCAGCTGTGGATGCCTCCTTAAGGTTATTTAGATCTGTTTTGAAAGGAGTTCAGTTGGAGTTGAAAAGGTCGTCTATCAGTTATGATGATATTATGTTTTGTTTGAATGCAATATTGAAGTTCGTAAAAAATACTTGTGAAAAAGTTAGTTCAGATTGTAGTGACAGAAATGACTTACATCACATGTCGCTTCAGTTGGTGGAGGCTGTCTGTGAGGAGATTGAACATACTATAGTGGGATCCCCTCTTTACAGTGTGCCATTTGACATCAAGTGTATTGATAACCTGCAAATATTAGTTGACATTGGATGTGCAAAATCCGGTGTCACTAACGGTTACATGGATATGGTTTCACCAATGGTTTATTTAAGTGTACTTTACTTCTGCTTAGTGGTTCAGTCAACAATGACCGAACCCAAAACAGACATCAATCTTCAGAGAATGCAGAATTATTTCAAATATCTGTTATCAATGTTTGATCCCCTGGAAAGCCTAGTTGTCACTAGTGGATTATTGTACAAGCACAGTGGTCCTAGCTGCTTAAGGATGTGGATTGCAGTGGCAGAAGGTTTGAAGTTCTACATTGATGATATGAAGGATTTTTCGTCATTGAAAATGGAATCTGACAGCAAATGTTGTTTTGCCATGTGCGATCTGTTGTCCTATCCACTAGTTGTATGCTCCTGTACTCCGAAATACTTTATGTCTGCAGAGCTTGGAAGCCCCTCAAAAGAATCTCATGCTTCACATCACATACAGGTTGAGCTTGAACAAGTTATCACACTCTGGAAGTCCCTCTATTGTTCTCTCTGCACCTCAATATCTGGGTGTTTCATTGGTGGCAGTTTCTTTGAGGATCTCTTTTCAATATTGGATAGGTGCCTTGAAAAGTGTGCTAGCATGCTTGTGTGTGCTGATGAGTATGTCTTACAATTCAAGGATCTGGCTCTTCATCGCATTTCCTCATATGGCGATGTGTTGATATGTATTTTGGAAAATTTCTGCTCTTCAGAATTTAATTCATATCGAGATAACAAGCATGGCTCTAAGCACAAGGTCTCTAGTGCCATTCAATTCTGCTTGAAATTGGTCATCAGATATATGAAGTTGATGCAGACAAAGATAGGATCAGACGAACCAATTGGTCTTAGTGCAGTCTCAAGGGTATATTCTGCATTGGCACACTTGCTGAGTTCCCTGCACTTGAAAGAGGATATCCTATCCTTCTTCAAGATAGTATCTGATCCACTGCTTCAGTGGCTGGTACTCATGGAAACACAAGATGAGAACACCAGCGATCAATTTCAACTCCTGTGGGCAGAAACGCTTAATTGTTTAAGGAGGAGTCAGCCTCCAATAATCTTTGACTCTGCATTTCTCCAACTCCAAGCACCCATCCTTGCAAAAACTCTTGATCACCCAAACCTGTCCATTTCAAATCCAACCATCACCTTTTGGAATTCCACATATGGCAAACAAACTGAGTTGGATTACCCCCAGACCTTACGTCATGTCTTAGACAAGCTCTGGAGAAATGGAAGAATAAGCCTCCATAAGAGAAGCCCACCACGTCAAAGATGTCAATCTAGACCGGGATCCGCTACCGCTCCACCAAGATACAGGGTCAATGCAACCCATAACAGAGGCTCAAAAAGAGTAGAACTGCAAGAGGACACGATAGGCGGTGAGCTCAAAGCAATGCCTCCATGTTCGAGTTTGAAAAGGAAAAGGTTGGAAGTAACTGAGCATCAGAAGGAAGTAAGGCGGGCACAGCAAGGAAGGGAGAGGGATTGTAGCGGTCACGGCCCGGGCATCCAGACTTTCACAAGCATGGATTTTTCGCAGGGGAACGAGGATACGCAAGACAACCAAGATATCCGGAATGCAGAGTGCATCTTGGAGCTGTTGAGAAAGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

1114

Amino Acids

125.74

Weight (kDa)

7.48

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rif1_N PF12231 94 - 277 6.6e-21 Rap1-interacting factor 1 N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1716
AccB1I GGYRCC 1 cut(s) 2301
AccB7I CCANNNNNTGG 1 cut(s) 1320
AccBSI CCGCTC 1 cut(s) 3029
AccI GTMKAC 3 cut(s) 389, 554, 992
AccII CGCG 1 cut(s) 148
AccIII TCCGGA 2 cut(s) 5, 3300
AciI CCGC 9 cut(s) 54, 148, 572, 691, 3021, 3027, 3105, 3187, 3219
AcsI RAATTY 5 cut(s) 747, 927, 2419, 2435, 2881
AcuI CTGAAG 6 cut(s) 33, 462, 1598, 1841, 2415, 2660
AcvI CACGTG 1 cut(s) 1035
AdeI CACNNNGTG 1 cut(s) 1038
AfaI GTAC 6 cut(s) 310, 1098, 1797, 1940, 2110, 2688
AfiI CCNNNNNNNGG 5 cut(s) 189, 697, 980, 1320, 1443
AflII CTTAAG 2 cut(s) 1441, 1963
AflIII ACRYGT 2 cut(s) 1032, 1605
AhdI GACNNNNNGTC 1 cut(s) 2928
AhlI ACTAGT 2 cut(s) 1925, 2092
AjiI CACGTC 1 cut(s) 2993
AjnI CCWGG 3 cut(s) 806, 1157, 1905
AjuI GAANNNNNNNTTGG 2 cut(s) 1282, 1314
Alw21I GWGCWC 1 cut(s) 3114
Alw26I GTCTC 4 cut(s) 854, 1408, 2485, 2584
AlwNI CAGNNNCTG 1 cut(s) 2683
Ama87I CYCGRG 1 cut(s) 3229
Aor13HI TCCGGA 2 cut(s) 5, 3300
AoxI GGCC 2 cut(s) 1155, 3226
ApeKI GCWGC 4 cut(s) 106, 153, 323, 1959
ApoI RAATTY 5 cut(s) 747, 927, 2419, 2435, 2881
AseI ATTAAT 1 cut(s) 732
Asp700I GAANNNNTTC 1 cut(s) 1874
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 3 cut(s) 1155, 1952, 3227
AsuC2I CCSGG 3 cut(s) 3015, 3230, 3231
AsuHPI GGTGA 5 cut(s) 827, 1767, 2831, 2863, 3119
AsuNHI GCTAGC 1 cut(s) 2318
AvaI CYCGRG 1 cut(s) 3229
AvaII GGWCC 1 cut(s) 1952
BaeGI GKGCMC 1 cut(s) 3193
BamHI GGATCC 2 cut(s) 1661, 3017
BanI GGYRCC 1 cut(s) 2301
BanII GRGCYC 2 cut(s) 475, 3114
BbrPI CACGTG 1 cut(s) 1035
BbsI GAAGAC 1 cut(s) 496
Bbv12I GWGCWC 1 cut(s) 3114
BbvI GCAGC 4 cut(s) 118, 140, 310, 1946
BccI CCATC 6 cut(s) 266, 675, 1251, 1322, 2825, 2876
BceAI ACGGC 1 cut(s) 3241
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 3 cut(s) 808, 1159, 1907
BciVI GTATCC 3 cut(s) 935, 1320, 3271
BclI TGATCA 1 cut(s) 2836
BcnI CCSGG 3 cut(s) 3015, 3230, 3231
BcoDI GTCTC 4 cut(s) 854, 1408, 2485, 2584
BcuI ACTAGT 2 cut(s) 1925, 2092
BfaI CTAG 9 cut(s) 1100, 1266, 1916, 1926, 1956, 2093, 2319, 2484, 3009
BfmI CTRYAG 2 cut(s) 1653, 2130
BfrI CTTAAG 2 cut(s) 1441, 1963
BfuAI ACCTGC 1 cut(s) 1716
BfuI GTATCC 3 cut(s) 935, 1320, 3271
BglII AGATCT 1 cut(s) 1454
BisI GCNGC 5 cut(s) 54, 107, 154, 324, 1960
BlsI GCNGC 5 cut(s) 55, 108, 155, 325, 1961
Bme1390I CCNGG 6 cut(s) 808, 1159, 1907, 3015, 3230, 3231
Bme18I GGWCC 1 cut(s) 1952
BmeRI GACNNNNNGTC 1 cut(s) 2928
BmeT110I CYCGRG 1 cut(s) 3229
BmgBI CACGTC 1 cut(s) 2993
BmgT120I GGNCC 3 cut(s) 1155, 1952, 3227
BmiI GGNNCC 5 cut(s) 161, 474, 1663, 2303, 3019
BmrFI CCNGG 6 cut(s) 808, 1159, 1907, 3015, 3230, 3231
BmrI ACTGGG 2 cut(s) 633, 707
BmtI GCTAGC 1 cut(s) 2322
BmuI ACTGGG 2 cut(s) 633, 707
BpiI GAAGAC 1 cut(s) 496
BplI GAGNNNNNCTC 2 cut(s) 2758, 2790
BpmI CTGGAG 2 cut(s) 86, 2971
Bpu10I CCTNAGC 2 cut(s) 497, 753
BpuEI CTTGAG 1 cut(s) 2566
BpuMI CCSGG 3 cut(s) 3015, 3230, 3231
BsaAI YACGTR 1 cut(s) 1035
BsaBI GATNNNNATC 2 cut(s) 983, 3004
BsaI GGTCTC 1 cut(s) 2485
BsaJI CCNNGG 6 cut(s) 585, 611, 807, 1328, 1905, 3229
BsaWI WCCGGW 3 cut(s) 5, 1742, 3300
Bsc4I CCNNNNNNNGG 5 cut(s) 189, 697, 980, 1320, 1443
Bse1I ACTGG 2 cut(s) 628, 702
Bse3DI GCAATG 1 cut(s) 3126
Bse8I GATNNNNATC 2 cut(s) 983, 3004
BseAI TCCGGA 2 cut(s) 5, 3300
BseBI CCWGG 3 cut(s) 808, 1159, 1907
BseDI CCNNGG 6 cut(s) 585, 611, 807, 1328, 1905, 3229
BseGI GGATG 9 cut(s) 165, 250, 633, 686, 1438, 1736, 1974, 2817, 3234
BseJI GATNNNNATC 2 cut(s) 983, 3004
BseLI CCNNNNNNNGG 5 cut(s) 189, 697, 980, 1320, 1443
BseMI GCAATG 1 cut(s) 3126
BseMII CTCAG 7 cut(s) 230, 744, 1084, 1328, 2603, 2895, 3156
BseNI ACTGG 2 cut(s) 628, 702
BseRI GAGGAG 2 cut(s) 1652, 2779
BseSI GKGCMC 1 cut(s) 3193
BseXI GCAGC 4 cut(s) 118, 140, 310, 1946
BsgI GTGCAG 3 cut(s) 2218, 2595, 2606
Bsh1236I CGCG 1 cut(s) 148
BshFI GGCC 2 cut(s) 1157, 3228
BshNI GGYRCC 1 cut(s) 2301
BsiHKAI GWGCWC 1 cut(s) 3114
BsiHKCI CYCGRG 1 cut(s) 3229
BsiSI CCGG 6 cut(s) 6, 157, 1743, 3014, 3230, 3301
BslFI GGGAC 1 cut(s) 2200
BslI CCNNNNNNNGG 5 cut(s) 189, 697, 980, 1320, 1443
BsmAI GTCTC 4 cut(s) 854, 1408, 2485, 2584
BsmFI GGGAC 1 cut(s) 2200
BsmI GAATGC 3 cut(s) 1534, 1869, 3310
BsnI GGCC 2 cut(s) 1157, 3228
Bso31I GGTCTC 1 cut(s) 2485
BsoBI CYCGRG 1 cut(s) 3229
Bsp1286I GDGCHC 3 cut(s) 475, 3114, 3193
Bsp13I TCCGGA 2 cut(s) 5, 3300
Bsp1407I TGTACA 1 cut(s) 1938
Bsp19I CCATGG 1 cut(s) 1328
BspACI CCGC 9 cut(s) 54, 148, 572, 691, 3021, 3027, 3105, 3187, 3219
BspANI GGCC 2 cut(s) 1157, 3228
BspCNI CTCAG 7 cut(s) 229, 745, 1083, 1329, 2604, 2896, 3157
BspEI TCCGGA 2 cut(s) 5, 3300
BspFNI CGCG 1 cut(s) 148
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 5 cut(s) 161, 474, 1663, 2303, 3019
BspMAI CTGCAG 1 cut(s) 2134
BspMI ACCTGC 1 cut(s) 1716
BspOI GCTAGC 1 cut(s) 2322
BspQI GCTCTTC 2 cut(s) 2376, 2434
BspT107I GGYRCC 1 cut(s) 2301
BspTI CTTAAG 2 cut(s) 1441, 1963
BspTNI GGTCTC 1 cut(s) 2485
BsrBI CCGCTC 1 cut(s) 3029
BsrDI GCAATG 1 cut(s) 3126
BsrGI TGTACA 1 cut(s) 1938
BsrI ACTGG 2 cut(s) 628, 702
BssECI CCNNGG 6 cut(s) 585, 611, 807, 1328, 1905, 3229
BssT1I CCWWGG 3 cut(s) 585, 611, 1328
Bst2UI CCWGG 3 cut(s) 808, 1159, 1907
Bst4CI ACNGT 6 cut(s) 844, 920, 1402, 1676, 1757, 1949
Bst6I CTCTTC 2 cut(s) 2376, 2434
BstAFI CTTAAG 2 cut(s) 1441, 1963
BstAPI GCANNNNNTGC 1 cut(s) 637
BstAUI TGTACA 1 cut(s) 1938
BstBAI YACGTR 1 cut(s) 1035
BstC8I GCNNGC 6 cut(s) 144, 348, 1024, 2320, 2324, 3189
BstDSI CCRYGG 1 cut(s) 1328
BstENI CCTNNNNNAGG 1 cut(s) 1441
BstF5I GGATG 9 cut(s) 165, 250, 633, 686, 1438, 1736, 1974, 2817, 3234
BstFNI CGCG 1 cut(s) 148
BstHHI GCGC 1 cut(s) 148
BstMAI GTCTC 4 cut(s) 854, 1408, 2485, 2584
BstNI CCWGG 3 cut(s) 808, 1159, 1907
BstNSI RCATGY 4 cut(s) 640, 909, 1609, 2326
BstSCI CCNGG 6 cut(s) 806, 1157, 1905, 3013, 3228, 3229
BstSFI CTRYAG 2 cut(s) 1653, 2130
BstSLI GKGCMC 1 cut(s) 3193
BstUI CGCG 1 cut(s) 148
BstV1I GCAGC 4 cut(s) 118, 140, 310, 1946
BstV2I GAAGAC 1 cut(s) 496
BstX2I RGATCY 7 cut(s) 701, 1333, 1454, 1661, 2278, 2362, 3017
BstYI RGATCY 7 cut(s) 701, 1333, 1454, 1661, 2278, 2362, 3017
BsuI GTATCC 3 cut(s) 935, 1320, 3271
BsuRI GGCC 2 cut(s) 1157, 3228
BtgI CCRYGG 1 cut(s) 1328
BtgZI GCGATG 2 cut(s) 2360, 2409
BtrI CACGTC 1 cut(s) 2993
BtsCI GGATG 9 cut(s) 165, 250, 633, 686, 1438, 1736, 1974, 2817, 3234
BtsI GCAGTG 2 cut(s) 1986, 2669
BtsIMutI CAGTG 7 cut(s) 974, 1078, 1681, 1954, 1986, 2669, 2684
BveI ACCTGC 1 cut(s) 1716
Cac8I GCNNGC 6 cut(s) 144, 348, 1024, 2320, 2324, 3189
CaiI CAGNNNCTG 1 cut(s) 2683
CciI TCATGA 1 cut(s) 772
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 3 cut(s) 1155, 1952, 3227
Cfr9I CCCGGG 1 cut(s) 3229
CseI GACGC 2 cut(s) 166, 1377
Csp6I GTAC 6 cut(s) 309, 1097, 1796, 1939, 2109, 2687
CspCI CAANNNNNGTGG 4 cut(s) 1930, 1965, 2717, 2752
CviQI GTAC 6 cut(s) 309, 1097, 1796, 1939, 2109, 2687
DraI TTTAAA 1 cut(s) 1359
DraIII CACNNNGTG 1 cut(s) 1038
DriI GACNNNNNGTC 1 cut(s) 2928
Eam1104I CTCTTC 2 cut(s) 2376, 2434
Eam1105I GACNNNNNGTC 1 cut(s) 2928
EarI CTCTTC 2 cut(s) 2376, 2434
Ecl136II GAGCTC 1 cut(s) 3112
Eco130I CCWWGG 3 cut(s) 585, 611, 1328
Eco24I GRGCYC 2 cut(s) 475, 3114
Eco31I GGTCTC 1 cut(s) 2485
Eco32I GATATC 2 cut(s) 2638, 3298
Eco47I GGWCC 1 cut(s) 1952
Eco53kI GAGCTC 1 cut(s) 3112
Eco57I CTGAAG 6 cut(s) 33, 462, 1598, 1841, 2415, 2660
Eco72I CACGTG 1 cut(s) 1035
Eco88I CYCGRG 1 cut(s) 3229
EcoICRI GAGCTC 1 cut(s) 3112
EcoNI CCTNNNNNAGG 1 cut(s) 1441
EcoRI GAATTC 1 cut(s) 2881
EcoRII CCWGG 3 cut(s) 806, 1157, 1905
EcoRV GATATC 2 cut(s) 2638, 3298
EcoT14I CCWWGG 3 cut(s) 585, 611, 1328
EcoT38I GRGCYC 2 cut(s) 475, 3114
ErhI CCWWGG 3 cut(s) 585, 611, 1328
FalI AAGNNNNNCTT 4 cut(s) 929, 961, 1282, 1314
FaqI GGGAC 1 cut(s) 2200
FauI CCCGC 1 cut(s) 3180
FauNDI CATATG 2 cut(s) 2389, 2890
FbaI TGATCA 1 cut(s) 2836
FblI GTMKAC 3 cut(s) 389, 554, 992
Fnu4HI GCNGC 5 cut(s) 54, 107, 154, 324, 1960
FokI GGATG 9 cut(s) 152, 237, 640, 693, 1445, 1743, 1981, 2804, 3221
FriOI GRGCYC 2 cut(s) 475, 3114
Fsp4HI GCNGC 5 cut(s) 54, 107, 154, 324, 1960
FspBI CTAG 9 cut(s) 1100, 1266, 1916, 1926, 1956, 2093, 2319, 2484, 3009
GlaI GCGC 1 cut(s) 147
GluI GCNGC 5 cut(s) 54, 107, 154, 324, 1960
GsuI CTGGAG 2 cut(s) 86, 2971
HaeIII GGCC 2 cut(s) 1157, 3228
HapII CCGG 6 cut(s) 6, 157, 1743, 3014, 3230, 3301
HgaI GACGC 2 cut(s) 166, 1377
HhaI GCGC 1 cut(s) 148
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 4 cut(s) 390, 993, 1723, 1824
HindII GTYRAC 4 cut(s) 390, 993, 1723, 1824
HpaII CCGG 6 cut(s) 6, 157, 1743, 3014, 3230, 3301
HphI GGTGA 5 cut(s) 827, 1767, 2831, 2863, 3119
Hpy99I CGWCG 2 cut(s) 182, 266
HpyAV CCTTC 8 cut(s) 486, 649, 806, 1982, 2011, 2656, 3169, 3195
HpyCH4III ACNGT 6 cut(s) 844, 920, 1402, 1676, 1757, 1949
HpyCH4IV ACGT 3 cut(s) 1034, 2929, 2992
HpySE526I ACGT 3 cut(s) 1034, 2929, 2992
HspAI GCGC 1 cut(s) 146
Kpn2I TCCGGA 2 cut(s) 5, 3300
Ksp22I TGATCA 1 cut(s) 2836
LguI GCTCTTC 2 cut(s) 2376, 2434
LmnI GCTCC 7 cut(s) 150, 159, 478, 1279, 2108, 3034, 3322
Lsp1109I GCAGC 4 cut(s) 118, 140, 310, 1946
MaeI CTAG 9 cut(s) 1100, 1266, 1916, 1926, 1956, 2093, 2319, 2484, 3009
MaeII ACGT 3 cut(s) 1034, 2929, 2992
MaeIII GTNAC 6 cut(s) 1583, 1747, 1757, 1921, 3160, 3221
MbiI CCGCTC 1 cut(s) 3029
MfeI CAATTG 2 cut(s) 302, 2562
MflI RGATCY 7 cut(s) 701, 1333, 1454, 1661, 2278, 2362, 3017
MhlI GDGCHC 3 cut(s) 475, 3114, 3193
MlyI GAGTC 5 cut(s) 486, 1217, 1348, 2776, 2783
MmeI TCCRAC 7 cut(s) 141, 322, 1458, 2827, 2888, 2889, 3135
MroI TCCGGA 2 cut(s) 5, 3300
MroXI GAANNNNTTC 1 cut(s) 1874
MslI CAYNNNNRTG 6 cut(s) 226, 679, 1091, 1766, 2327, 3251
MspA1I CMGCKG 1 cut(s) 1427
MspCI CTTAAG 2 cut(s) 1441, 1963
MspI CCGG 6 cut(s) 6, 157, 1743, 3014, 3230, 3301
MspR9I CCNGG 6 cut(s) 808, 1159, 1907, 3015, 3230, 3231
MunI CAATTG 2 cut(s) 302, 2562
Mva1269I GAATGC 3 cut(s) 1534, 1869, 3310
MvaI CCWGG 3 cut(s) 808, 1159, 1907
MvnI CGCG 1 cut(s) 148
NciI CCSGG 3 cut(s) 3015, 3230, 3231
NcoI CCATGG 1 cut(s) 1328
NdeI CATATG 2 cut(s) 2389, 2890
NheI GCTAGC 1 cut(s) 2318
NlaIV GGNNCC 5 cut(s) 161, 474, 1663, 2303, 3019
NmuCI GTSAC 4 cut(s) 1583, 1747, 1921, 3221
NspI RCATGY 4 cut(s) 640, 909, 1609, 2326
PacI TTAATTAA 1 cut(s) 732
PaeI GCATGC 1 cut(s) 2326
PagI TCATGA 1 cut(s) 772
PciI ACATGT 1 cut(s) 1605
PciSI GCTCTTC 2 cut(s) 2376, 2434
PctI GAATGC 3 cut(s) 1534, 1869, 3310
PdmI GAANNNNTTC 1 cut(s) 1874
PfeI GAWTC 6 cut(s) 128, 776, 1076, 1162, 2042, 2156
PflMI CCANNNNNTGG 1 cut(s) 1320
PkrI GCNGC 5 cut(s) 55, 108, 155, 325, 1961
PleI GAGTC 5 cut(s) 486, 1217, 1347, 2775, 2783
PmaCI CACGTG 1 cut(s) 1035
PmlI CACGTG 1 cut(s) 1035
PpsI GAGTC 5 cut(s) 486, 1217, 1347, 2775, 2783
Ppu21I YACGTR 1 cut(s) 1035
PscI ACATGT 1 cut(s) 1605
PshBI ATTAAT 1 cut(s) 732
Psp124BI GAGCTC 1 cut(s) 3114
Psp6I CCWGG 3 cut(s) 806, 1157, 1905
PspCI CACGTG 1 cut(s) 1035
PspGI CCWGG 3 cut(s) 806, 1157, 1905
PspN4I GGNNCC 5 cut(s) 161, 474, 1663, 2303, 3019
PspPI GGNCC 3 cut(s) 1155, 1952, 3227
PstI CTGCAG 1 cut(s) 2134
PstNI CAGNNNCTG 1 cut(s) 2683
PsuI RGATCY 7 cut(s) 701, 1333, 1454, 1661, 2278, 2362, 3017
PvuII CAGCTG 1 cut(s) 1427
RsaI GTAC 6 cut(s) 310, 1098, 1797, 1940, 2110, 2688
RsaNI GTAC 6 cut(s) 309, 1097, 1796, 1939, 2109, 2687
RseI CAYNNNNRTG 6 cut(s) 226, 679, 1091, 1766, 2327, 3251
SacI GAGCTC 1 cut(s) 3114
SalI GTCGAC 2 cut(s) 388, 991
SapI GCTCTTC 2 cut(s) 2376, 2434
SatI GCNGC 5 cut(s) 54, 107, 154, 324, 1960
Sau96I GGNCC 3 cut(s) 1155, 1952, 3227
SchI GAGTC 5 cut(s) 486, 1217, 1348, 2776, 2783
ScrFI CCNGG 6 cut(s) 808, 1159, 1907, 3015, 3230, 3231
SduI GDGCHC 3 cut(s) 475, 3114, 3193
SfcI CTRYAG 2 cut(s) 1653, 2130
SinI GGWCC 1 cut(s) 1952
SmaI CCCGGG 1 cut(s) 3231
SmiMI CAYNNNNRTG 6 cut(s) 226, 679, 1091, 1766, 2327, 3251
SmlI CTYRAG 3 cut(s) 1441, 1963, 2581
SmoI CTYRAG 3 cut(s) 1441, 1963, 2581
SpeI ACTAGT 2 cut(s) 1925, 2092
SphI GCATGC 1 cut(s) 2326
SrfI GCCCGGGC 1 cut(s) 3231
SsiI CCGC 9 cut(s) 54, 148, 572, 691, 3021, 3027, 3105, 3187, 3219
SspI AATATT 4 cut(s) 561, 1536, 1716, 2292
SspMI CTAG 9 cut(s) 1100, 1266, 1916, 1926, 1956, 2093, 2319, 2484, 3009
SstI GAGCTC 1 cut(s) 3114
StyD4I CCNGG 6 cut(s) 806, 1157, 1905, 3013, 3228, 3229
StyI CCWWGG 3 cut(s) 585, 611, 1328
TaaI ACNGT 6 cut(s) 844, 920, 1402, 1676, 1757, 1949
TaiI ACGT 3 cut(s) 1037, 2932, 2995
TaqI TCGA 5 cut(s) 24, 389, 992, 2449, 3134
TaqII GACCGA 2 cut(s) 540, 1847
TatI WGTACW 4 cut(s) 308, 1795, 1938, 2108
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 6 cut(s) 128, 776, 1076, 1162, 2042, 2156
TscAI CASTG 7 cut(s) 981, 1078, 1681, 1954, 1986, 2676, 2684
TseFI GTSAC 4 cut(s) 1583, 1747, 1921, 3221
TseI GCWGC 4 cut(s) 106, 153, 323, 1959
Tsp45I GTSAC 4 cut(s) 1583, 1747, 1921, 3221
TspDTI ATGAA 9 cut(s) 347, 434, 668, 1180, 2030, 2245, 2363, 2433, 2540
TspGWI ACGGA 2 cut(s) 158, 243
TspMI CCCGGG 1 cut(s) 3229
TspRI CASTG 7 cut(s) 981, 1078, 1681, 1954, 1986, 2676, 2684
Van91I CCANNNNNTGG 1 cut(s) 1320
Vha464I CTTAAG 2 cut(s) 1441, 1963
VpaK11BI GGWCC 1 cut(s) 1952
VspI ATTAAT 1 cut(s) 732
XagI CCTNNNNNAGG 1 cut(s) 1441
XapI RAATTY 5 cut(s) 747, 927, 2419, 2435, 2881
XbaI TCTAGA 1 cut(s) 3008
XceI RCATGY 4 cut(s) 640, 909, 1609, 2326
XcmI CCANNNNNNNNNTGG 1 cut(s) 2676
XmaI CCCGGG 1 cut(s) 3229
XmiI GTMKAC 3 cut(s) 389, 554, 992
XmnI GAANNNNTTC 1 cut(s) 1874
XspI CTAG 9 cut(s) 1100, 1266, 1916, 1926, 1956, 2093, 2319, 2484, 3009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.