pycom15g21060

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007911
Physical Location & Seq
Reverse (-)
15087589 .. 15090178
2590 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
ATGAGTAATGTTAGTGGACCAATTTTTAAACTATATTTCACATTGATTAAAAAAGAGTTAAGAAGGCAACCAGCCGAAGTTAAGGTCAACTCTCTCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAGACGTGGATATTTTGGTTTGGTGCAATGCTCGGAGCCGGAGCTCAATTCCACGTGACGGATCGCTCAGCTGCCATCCATCCGGTCCCAAAAACTTCGTCTTCTGCTTCCTCTTCGCGCCACTTGGTCTTCACGGTTCGATTAGGGTTCATCAGACCATCAAAACGGTGTCGTGCGCTCAAATCTCTCCGCATTACCTGCAACAGCTCTTCCTCTCGCTCTAGATCCAGCAATGCCGACGATCATCACGATTTCGACCACATCCAAGCGTCCCTCCTCCTCTCAGAAACTATATCGCACTACCGTATGCAGAGGCGAGGGTTTCAAGAAGAATCGAAATGGCAGTTATCTGGTAAATTGCGTCCCTTTTCGGTCCACGCCAGCCGACAACAAAACATTCTTGATACATTAGGCCGAAACTTTGTCCAGCGTTTCCAAACTCCCACGATTTTTCTTAAGATTTCTTGTGATGGAGACTTTTTGCTGCCAATTGTTGTAGGCGAATTTGCAATAGAAAATCTCATAGATGCCCAGTGGGGAGATGAGAATGGGGACTCCCCAGATCATTTCCAGTTCTTAAGAAACCTTGTTGACAAACTCGGCTATCAAGTGAATATGGTGAGAATCACGGAAAGAGTGCGGAATACTTACTTTGCCAGATTGTACTTTAGCAAGCCAGGGGAAAATGGCTTTCTTAGTGTGGATGCACGCCCGTCAGATGCCATAAATTTCGCACATAGATGCAAGGCACCCATATATATAAACAAACAGATTGTCTTAACAGATGCAGTGAGGATCAGTTATGGAATGGGCAGAGTGCGCGATACAAAGTCTTGTTATGACGTATCTCTTGACAGTGCTATAGAGGGCCCAGATTCTTTAATTCAAGAACTTGGTTTGGTTAAGAATATCAATTTAGCTATCAAAGAGGAAAGATATAAAGATGCAGCCATGTGGAAAGACGAACTATTCAAGCTTCGCAAATCAATTCAAGGGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

376

Amino Acids

42.57

Weight (kDa)

9.62

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 200 - 306 1.3e-19 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 874
AccII CGCG 2 cut(s) 244, 948
AciI CCGC 2 cut(s) 316, 766
AclWI GGATC 3 cut(s) 195, 345, 929
AcsI RAATTY 2 cut(s) 629, 853
AcvI CACGTG 1 cut(s) 181
AfaI GTAC 1 cut(s) 791
AfiI CCNNNNNNNGG 1 cut(s) 184
AflII CTTAAG 2 cut(s) 581, 703
AgsI TTSAA 4 cut(s) 452, 1013, 1099, 1118
AjiI CACGTC 1 cut(s) 132
AjnI CCWGG 1 cut(s) 802
AluBI AGCT 5 cut(s) 170, 197, 333, 1046, 1102
AluI AGCT 5 cut(s) 170, 197, 333, 1046, 1102
Alw21I GWGCWC 1 cut(s) 172
Alw26I GTCTC 1 cut(s) 594
AlwI GGATC 3 cut(s) 195, 345, 929
AoxI GGCC 3 cut(s) 538, 994, 1121
ApaI GGGCCC 1 cut(s) 998
ApeKI GCWGC 3 cut(s) 197, 610, 1073
ApoI RAATTY 2 cut(s) 629, 853
ArsI GACNNNNNNTTYG 6 cut(s) 69, 101, 209, 241, 885, 917
AspLEI GCGC 3 cut(s) 246, 304, 948
AspS9I GGNCC 6 cut(s) 17, 211, 499, 994, 995, 1121
AsuHPI GGTGA 1 cut(s) 757
AvaII GGWCC 3 cut(s) 17, 211, 499
BaeGI GKGCMC 1 cut(s) 998
BanI GGYRCC 1 cut(s) 874
BanII GRGCYC 2 cut(s) 172, 998
BbrPI CACGTG 1 cut(s) 181
BbsI GAAGAC 2 cut(s) 219, 247
Bbv12I GWGCWC 1 cut(s) 172
BbvI GCAGC 3 cut(s) 184, 597, 1085
BccI CCATC 4 cut(s) 209, 213, 292, 590
BciT130I CCWGG 1 cut(s) 804
BcoDI GTCTC 1 cut(s) 594
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 987
BfrI CTTAAG 2 cut(s) 581, 703
BfuAI ACCTGC 1 cut(s) 332
BisI GCNGC 3 cut(s) 198, 611, 1074
BlpI GCTNAGC 1 cut(s) 193
BlsI GCNGC 3 cut(s) 199, 612, 1075
Bme1390I CCNGG 1 cut(s) 804
Bme18I GGWCC 3 cut(s) 17, 211, 499
BmgBI CACGTC 1 cut(s) 132
BmgT120I GGNCC 6 cut(s) 17, 211, 499, 994, 995, 1121
BmiI GGNNCC 4 cut(s) 163, 213, 876, 996
BmrFI CCNGG 1 cut(s) 804
BmrI ACTGGG 1 cut(s) 652
BmsI GCATC 6 cut(s) 643, 820, 835, 857, 901, 1060
BmuI ACTGGG 1 cut(s) 652
BpiI GAAGAC 2 cut(s) 219, 247
Bpu1102I GCTNAGC 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 181
BsaJI CCNNGG 1 cut(s) 803
BsaWI WCCGGW 1 cut(s) 208
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse1I ACTGG 2 cut(s) 658, 697
Bse3DI GCAATG 2 cut(s) 159, 364
BseBI CCWGG 1 cut(s) 804
BseDI CCNNGG 1 cut(s) 803
BseGI GGATG 4 cut(s) 201, 205, 387, 835
BseLI CCNNNNNNNGG 1 cut(s) 184
BseMI GCAATG 2 cut(s) 159, 364
BseMII CTCAG 3 cut(s) 140, 207, 423
BseNI ACTGG 2 cut(s) 658, 697
BseRI GAGGAG 3 cut(s) 88, 392, 395
BseSI GKGCMC 1 cut(s) 998
BseXI GCAGC 3 cut(s) 184, 597, 1085
Bsh1236I CGCG 2 cut(s) 244, 948
BshFI GGCC 3 cut(s) 540, 996, 1123
BshNI GGYRCC 1 cut(s) 874
BsiHKAI GWGCWC 1 cut(s) 172
BsiSI CCGG 2 cut(s) 165, 209
BslFI GGGAC 4 cut(s) 197, 382, 474, 692
BslI CCNNNNNNNGG 1 cut(s) 184
BsmAI GTCTC 1 cut(s) 594
BsmFI GGGAC 4 cut(s) 197, 382, 474, 692
BsnI GGCC 3 cut(s) 540, 996, 1123
Bsp120I GGGCCC 1 cut(s) 994
Bsp1286I GDGCHC 2 cut(s) 172, 998
Bsp143I GATC 5 cut(s) 187, 350, 367, 688, 921
Bsp1720I GCTNAGC 1 cut(s) 193
BspACI CCGC 2 cut(s) 316, 766
BspANI GGCC 3 cut(s) 540, 996, 1123
BspCNI CTCAG 3 cut(s) 139, 206, 422
BspFNI CGCG 2 cut(s) 244, 948
BspLI GGNNCC 4 cut(s) 163, 213, 876, 996
BspMI ACCTGC 1 cut(s) 332
BspPI GGATC 3 cut(s) 195, 345, 929
BspQI GCTCTTC 1 cut(s) 340
BspT107I GGYRCC 1 cut(s) 874
BspTI CTTAAG 2 cut(s) 581, 703
BsrDI GCAATG 2 cut(s) 159, 364
BsrI ACTGG 2 cut(s) 658, 697
BssECI CCNNGG 1 cut(s) 803
BssMI GATC 5 cut(s) 187, 350, 367, 688, 921
Bst2UI CCWGG 1 cut(s) 804
Bst4CI ACNGT 4 cut(s) 262, 294, 431, 983
Bst6I CTCTTC 2 cut(s) 244, 340
BstAFI CTTAAG 2 cut(s) 581, 703
BstAPI GCANNNNNTGC 1 cut(s) 324
BstBAI YACGTR 1 cut(s) 181
BstC8I GCNNGC 3 cut(s) 508, 800, 835
BstDEI CTNAG 4 cut(s) 126, 193, 409, 821
BstF5I GGATG 4 cut(s) 201, 205, 387, 835
BstFNI CGCG 2 cut(s) 244, 948
BstHHI GCGC 3 cut(s) 246, 304, 948
BstKTI GATC 5 cut(s) 190, 353, 370, 691, 924
BstMAI GTCTC 1 cut(s) 594
BstMBI GATC 5 cut(s) 187, 350, 367, 688, 921
BstMWI GCNNNNNNNGC 2 cut(s) 324, 945
BstNI CCWGG 1 cut(s) 804
BstSCI CCNGG 1 cut(s) 802
BstSFI CTRYAG 1 cut(s) 987
BstSLI GKGCMC 1 cut(s) 998
BstUI CGCG 2 cut(s) 244, 948
BstV1I GCAGC 3 cut(s) 184, 597, 1085
BstV2I GAAGAC 2 cut(s) 219, 247
BstX2I RGATCY 1 cut(s) 350
BstYI RGATCY 1 cut(s) 350
BsuRI GGCC 3 cut(s) 540, 996, 1123
BtrI CACGTC 1 cut(s) 132
BtsCI GGATG 4 cut(s) 201, 205, 387, 835
BtsI GCAGTG 1 cut(s) 921
BtsIMutI CAGTG 3 cut(s) 665, 921, 988
BveI ACCTGC 1 cut(s) 332
Cac8I GCNNGC 3 cut(s) 508, 800, 835
CfoI GCGC 3 cut(s) 246, 304, 948
Cfr13I GGNCC 6 cut(s) 17, 211, 499, 994, 995, 1121
CseI GACGC 2 cut(s) 384, 476
Csp6I GTAC 1 cut(s) 790
CviAII CATG 1 cut(s) 1078
CviQI GTAC 1 cut(s) 790
DdeI CTNAG 4 cut(s) 126, 193, 409, 821
DpnI GATC 5 cut(s) 189, 352, 369, 690, 923
DpnII GATC 5 cut(s) 187, 350, 367, 688, 921
DraI TTTAAA 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 244, 340
EarI CTCTTC 2 cut(s) 244, 340
Ecl136II GAGCTC 1 cut(s) 170
Eco24I GRGCYC 2 cut(s) 172, 998
Eco47I GGWCC 3 cut(s) 17, 211, 499
Eco53kI GAGCTC 1 cut(s) 170
Eco72I CACGTG 1 cut(s) 181
EcoICRI GAGCTC 1 cut(s) 170
EcoO109I RGGNCCY 2 cut(s) 994, 1121
EcoRII CCWGG 1 cut(s) 802
EcoT38I GRGCYC 2 cut(s) 172, 998
FaeI CATG 1 cut(s) 1081
FaqI GGGAC 4 cut(s) 197, 382, 474, 692
FatI CATG 1 cut(s) 1077
Fnu4HI GCNGC 3 cut(s) 198, 611, 1074
FokI GGATG 4 cut(s) 188, 192, 374, 842
FriOI GRGCYC 2 cut(s) 172, 998
Fsp4HI GCNGC 3 cut(s) 198, 611, 1074
FspBI CTAG 1 cut(s) 348
GlaI GCGC 3 cut(s) 245, 303, 947
GluI GCNGC 3 cut(s) 198, 611, 1074
HaeIII GGCC 3 cut(s) 540, 996, 1123
HapII CCGG 2 cut(s) 165, 209
HgaI GACGC 2 cut(s) 384, 476
HhaI GCGC 3 cut(s) 246, 304, 948
Hin1II CATG 1 cut(s) 1081
Hin6I GCGC 3 cut(s) 244, 302, 946
HinP1I GCGC 3 cut(s) 244, 302, 946
HincII GTYRAC 2 cut(s) 88, 718
HindII GTYRAC 2 cut(s) 88, 718
HindIII AAGCTT 1 cut(s) 1100
HinfI GANTC 4 cut(s) 458, 680, 750, 1001
HpaII CCGG 2 cut(s) 165, 209
HphI GGTGA 1 cut(s) 757
Hpy166II GTNNAC 4 cut(s) 17, 88, 502, 718
Hpy188I TCNGA 5 cut(s) 129, 161, 281, 412, 844
Hpy188III TCNNGA 6 cut(s) 348, 374, 452, 527, 977, 1013
Hpy8I GTNNAC 4 cut(s) 17, 88, 502, 718
Hpy99I CGWCG 1 cut(s) 368
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 4 cut(s) 262, 294, 431, 983
HpyCH4IV ACGT 3 cut(s) 131, 180, 969
HpyCH4V TGCA 8 cut(s) 152, 327, 436, 635, 833, 870, 914, 1073
HpyF10VI GCNNNNNNNGC 2 cut(s) 324, 945
HpyF3I CTNAG 4 cut(s) 126, 193, 409, 821
HpySE526I ACGT 3 cut(s) 131, 180, 969
Hsp92II CATG 1 cut(s) 1081
HspAI GCGC 3 cut(s) 244, 302, 946
Kzo9I GATC 5 cut(s) 187, 350, 367, 688, 921
LguI GCTCTTC 1 cut(s) 340
LmnI GCTCC 2 cut(s) 161, 167
Lsp1109I GCAGC 3 cut(s) 184, 597, 1085
LweI GCATC 6 cut(s) 643, 820, 835, 857, 901, 1060
MaeI CTAG 1 cut(s) 348
MaeII ACGT 3 cut(s) 131, 180, 969
MaeIII GTNAC 1 cut(s) 181
MalI GATC 5 cut(s) 189, 352, 369, 690, 923
MboI GATC 5 cut(s) 187, 350, 367, 688, 921
MboII GAAGA 5 cut(s) 219, 231, 247, 327, 467
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 1 cut(s) 350
MhlI GDGCHC 2 cut(s) 172, 998
MluCI AATT 9 cut(s) 21, 173, 482, 615, 629, 853, 1008, 1039, 1113
MlyI GAGTC 1 cut(s) 674
MslI CAYNNNNRTG 1 cut(s) 865
MspA1I CMGCKG 1 cut(s) 197
MspCI CTTAAG 2 cut(s) 581, 703
MspI CCGG 2 cut(s) 165, 209
MspR9I CCNGG 1 cut(s) 804
MunI CAATTG 1 cut(s) 615
MvaI CCWGG 1 cut(s) 804
MvnI CGCG 2 cut(s) 244, 948
MwoI GCNNNNNNNGC 2 cut(s) 324, 945
NdeII GATC 5 cut(s) 187, 350, 367, 688, 921
NlaIII CATG 1 cut(s) 1081
NlaIV GGNNCC 4 cut(s) 163, 213, 876, 996
NmeAIII GCCGAG 1 cut(s) 705
NmuCI GTSAC 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 340
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PfeI GAWTC 3 cut(s) 458, 750, 1001
PkrI GCNGC 3 cut(s) 199, 612, 1075
PleI GAGTC 1 cut(s) 674
PmaCI CACGTG 1 cut(s) 181
PmlI CACGTG 1 cut(s) 181
PpsI GAGTC 1 cut(s) 674
Ppu21I YACGTR 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 172
Psp6I CCWGG 1 cut(s) 802
PspCI CACGTG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 802
PspN4I GGNNCC 4 cut(s) 163, 213, 876, 996
PspOMI GGGCCC 1 cut(s) 994
PspPI GGNCC 6 cut(s) 17, 211, 499, 994, 995, 1121
PsuI RGATCY 1 cut(s) 350
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 1 cut(s) 791
RsaNI GTAC 1 cut(s) 790
RseI CAYNNNNRTG 1 cut(s) 865
SacI GAGCTC 1 cut(s) 172
SapI GCTCTTC 1 cut(s) 340
SatI GCNGC 3 cut(s) 198, 611, 1074
Sau3AI GATC 5 cut(s) 187, 350, 367, 688, 921
Sau96I GGNCC 6 cut(s) 17, 211, 499, 994, 995, 1121
SchI GAGTC 1 cut(s) 674
ScrFI CCNGG 1 cut(s) 804
SduI GDGCHC 2 cut(s) 172, 998
SfaNI GCATC 6 cut(s) 643, 820, 835, 857, 901, 1060
SfcI CTRYAG 1 cut(s) 987
SinI GGWCC 3 cut(s) 17, 211, 499
SmiMI CAYNNNNRTG 1 cut(s) 865
SmlI CTYRAG 2 cut(s) 581, 703
SmoI CTYRAG 2 cut(s) 581, 703
Sse9I AATT 9 cut(s) 21, 173, 482, 615, 629, 853, 1008, 1039, 1113
SsiI CCGC 2 cut(s) 316, 766
SspMI CTAG 1 cut(s) 348
SstI GAGCTC 1 cut(s) 172
StyD4I CCNGG 1 cut(s) 802
TaaI ACNGT 4 cut(s) 262, 294, 431, 983
TaiI ACGT 3 cut(s) 134, 183, 972
TaqI TCGA 3 cut(s) 265, 381, 461
TaqII GACCGA 1 cut(s) 487
TasI AATT 9 cut(s) 21, 173, 482, 615, 629, 853, 1008, 1039, 1113
TatI WGTACW 1 cut(s) 789
TfiI GAWTC 3 cut(s) 458, 750, 1001
TscAI CASTG 3 cut(s) 665, 921, 988
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 3 cut(s) 197, 610, 1073
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 1 cut(s) 265
TspGWI ACGGA 2 cut(s) 200, 770
TspRI CASTG 3 cut(s) 665, 921, 988
Vha464I CTTAAG 2 cut(s) 581, 703
VpaK11BI GGWCC 3 cut(s) 17, 211, 499
XapI RAATTY 2 cut(s) 629, 853
XbaI TCTAGA 1 cut(s) 347
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.