Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0002579

Physical Location & Seq

Genomic Coordinates Reverse (-)

16327488 .. 16332119

Gene Structure 4632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g22230.8

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGCAGAGCCGTCGACGCCGTTTCGCCTCACCTTCTTCTCCTTCTTCCTCGCATCGCACTTTTCTTGCCCTACTTACAGTTCTTGCATCGCAAGCACTTCAAGCTTCTGCAGTTGTAGTACCGCGATCCAATTGCTACGTTTTCGATAACACTAGCCGCCTTGTCGATTTTAGCAGCTGGATTGGGCATCCGTTTGAGTACGACGGAGGGGAAACCGACTTGGTGATTCGGTTTTGCAAAGATGTGGAGAGTAGATCACAAACGGGATATGTAGATTTCGGTCGATTTGATAAGTACAACAACTTTGTTTCTGGTTCAGGACACGTAGACTATGTTCAAGGGTTTTACAATGGAGACCTGGCAAATTGCGAGACCAGTTATGATAAACTGGGACGAACAGCTCAGGTAAACATTATATGTGGAAGTTGTTTAAATGGACAATGCAAAGGTGGACTTGGATGCATCTGCAATGTCACTTACGAGTCAACTTGCAGAGTTTTTCTAGAGCTGGCCATTCCATGTGAGAAACCAGGACCCCGCGTATTTAAGGGCTTCACTGTAGGTTTCCATCCACGGTCATGGGAGCTTGTTTATGATGGCATGACCCAAATAGGCTATGAAAAATCTCACCGTGAGTTTAGCTTTGGGACCGAGCAGAGACATGTAACCCTTTATATGACTGCAATGGCTTCCCTATCCACTTTGGTGCAAAGACCTATTATTAAGGTCTTACCAGAAAGTGGCCTGGAGATTAAATTATCGGGGTCGGGGGCAACTGGGAGCGCGCCTACAACTTTGTCACCGACAATGTTAAATGTTGAATGGAGATGTGAAAGGGCACGTGATACTCCGTATGAAGTGAATATCACTATCCCAGTGGAGGGTTATGAGCCCATCCAGTTTTTTCTTGCCAAAATGTGTGAACATAGGCAGGAGAAGAGTGGAGAGAGTACGACGGGATGGGCTACATTTGGGGTGTTGTCCTGCATATTCACTGTATCATCAACACTGTTTTGTTGTGGAGGGTTTATTTACAAGACTCGAGTTGAACAGAAGCGTGGGATAGACGCATTGCCTGGCATGACAATTCTATCTGCTTGTTTAGAAACTGTGAGTGACACAGGGCAAAGCTACACCCGACACGAAGATCTTAATACTGCCTTTGCCAGTGAAGCCTCTTGGGAACGTTCTCCTTCTCCTGCTCCTCCAACTAAAGGAACATCGAAGCCGAATGAAAGCAGATACGGTGCAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

419

Amino Acids

46.17

Weight (kDa)

6.71

Isoelectric Point (pI)

44.48

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002579)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36440
fragaria_vesca	FvH4_1g12510 FvH4_1g12510 FvH4_1g12510 FvH4_1g12510 FvH4_1g12510 FvH4_1g12510
malus_domestica	MD02G1139000.v1.1 MD15G1252400.v1.1
prunus_persica	Prupe.7G161800_v2.0.a1
pyrus_communis	pycom15g22230
rosa_chinensis	RchiOBHm_Chr2g0100411 RchiOBHm_Chr2g0136281
rosa_laevigata	RLG00000016971 RLG00000016995
rosa_multiflora	Rmu_sc0001095.1_g000010 Rmu_sc0005038.1_g000024 Rmu_sc0005093.1_g000022 Rmu_ssc0000008.1_g000012
rosa_roxburghii	Rroxscaffold_2G00095660 Rroxscaffold_2G00142270 Rroxscaffold_3G00237860 Rroxscaffold_4G00320340 Rroxscaffold_7G00196390
rosa_rugosa	Rorug02G0087900 Rorug02G0088000 Rorug02G0088100
rosa_samantha	Rh2AG137400 Rh2AG137600 Rh2BG140600 Rh2BG140800 Rh2CG142700 Rh2DG142600 Rh3DG330200 Rh4AG080100 Rh4CG086200 Rh4CG256300 Rh5AG182400
rosa_wichuraiana	Rw2G010750 Rw7G016510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	740
AccI	GTMKAC	2 cut(s)	13, 327
AccII	CGCG	3 cut(s)	124, 540, 785
AciI	CCGC	3 cut(s)	122, 157, 538
AclI	AACGTT	1 cut(s)	1186
AclWI	GGATC	1 cut(s)	120
AcoI	YGGCCR	1 cut(s)	510
AcvI	CACGTG	1 cut(s)	842
AcyI	GRCGYC	1 cut(s)	16
AfaI	GTAC	4 cut(s)	120, 200, 296, 952
AfiI	CCNNNNNNNGG	4 cut(s)	740, 880, 881, 1214
AflIII	ACRYGT	2 cut(s)	322, 661
AgsI	TTSAA	4 cut(s)	101, 338, 821, 1049
AjnI	CCWGG	4 cut(s)	357, 529, 744, 1075
AleI	CACNNNNGTG	1 cut(s)	704
AluBI	AGCT	7 cut(s)	104, 177, 401, 508, 586, 642, 1131
AluI	AGCT	7 cut(s)	104, 177, 401, 508, 586, 642, 1131
Alw26I	GTCTC	3 cut(s)	348, 365, 652
AlwI	GGATC	1 cut(s)	120
Ama87I	CYCGRG	1 cut(s)	1041
AoxI	GGCC	2 cut(s)	510, 742
ApeKI	GCWGC	1 cut(s)	174
AspLEI	GCGC	2 cut(s)	785, 787
AspS9I	GGNCC	2 cut(s)	533, 648
AsuHPI	GGTGA	4 cut(s)	21, 235, 620, 792
AvaI	CYCGRG	1 cut(s)	1041
AvaII	GGWCC	2 cut(s)	533, 648
BaeGI	GKGCMC	1 cut(s)	841
BalI	TGGCCA	1 cut(s)	512
BanII	GRGCYC	1 cut(s)	894
BbrPI	CACGTG	1 cut(s)	842
BbvI	GCAGC	1 cut(s)	186
BccI	CCATC	4 cut(s)	576, 590, 902, 954
BceAI	ACGGC	1 cut(s)	3
BcgI	CGANNNNNNTGC	3 cut(s)	27, 114, 148
BciT130I	CCWGG	4 cut(s)	359, 531, 746, 1077
BcoDI	GTCTC	3 cut(s)	348, 365, 652
BfaI	CTAG	2 cut(s)	153, 503
BfmI	CTRYAG	2 cut(s)	108, 558
BglII	AGATCT	1 cut(s)	1147
BisI	GCNGC	2 cut(s)	157, 175
BlsI	GCNGC	2 cut(s)	158, 176
Bme1390I	CCNGG	4 cut(s)	359, 531, 746, 1077
Bme18I	GGWCC	2 cut(s)	533, 648
BmeT110I	CYCGRG	1 cut(s)	1041
BmgT120I	GGNCC	2 cut(s)	533, 648
BmiI	GGNNCC	2 cut(s)	535, 649
BmrFI	CCNGG	4 cut(s)	359, 531, 746, 1077
BmrI	ACTGGG	3 cut(s)	398, 786, 869
BmsI	GCATC	5 cut(s)	61, 95, 196, 449, 471
BmuI	ACTGGG	3 cut(s)	398, 786, 869
BpmI	CTGGAG	1 cut(s)	767
Bpu10I	CCTNAGC	1 cut(s)	402
BsaAI	YACGTR	2 cut(s)	325, 842
BsaHI	GRCGYC	1 cut(s)	16
BsaI	GGTCTC	2 cut(s)	348, 365
BsaJI	CCNNGG	1 cut(s)	572
Bsc4I	CCNNNNNNNGG	4 cut(s)	740, 880, 881, 1214
Bse1I	ACTGG	6 cut(s)	375, 393, 781, 875, 898, 1167
Bse3DI	GCAATG	3 cut(s)	475, 690, 1070
BseBI	CCWGG	4 cut(s)	359, 531, 746, 1077
BseDI	CCNNGG	1 cut(s)	572
BseGI	GGATG	5 cut(s)	187, 464, 568, 894, 965
BseLI	CCNNNNNNNGG	4 cut(s)	740, 880, 881, 1214
BseMI	GCAATG	3 cut(s)	475, 690, 1070
BseMII	CTCAG	1 cut(s)	416
BseNI	ACTGG	6 cut(s)	375, 393, 781, 875, 898, 1167
BsePI	GCGCGC	1 cut(s)	783
BseRI	GAGGAG	1 cut(s)	1194
BseSI	GKGCMC	1 cut(s)	841
BseXI	GCAGC	1 cut(s)	186
Bsh1236I	CGCG	3 cut(s)	124, 540, 785
Bsh1285I	CGRYCG	1 cut(s)	283
BshFI	GGCC	2 cut(s)	512, 744
BsiEI	CGRYCG	1 cut(s)	283
BsiHKCI	CYCGRG	1 cut(s)	1041
BslFI	GGGAC	2 cut(s)	405, 661
BslI	CCNNNNNNNGG	4 cut(s)	740, 880, 881, 1214
BsmAI	GTCTC	3 cut(s)	348, 365, 652
BsmFI	GGGAC	2 cut(s)	405, 661
BsnI	GGCC	2 cut(s)	512, 744
Bso31I	GGTCTC	2 cut(s)	348, 365
BsoBI	CYCGRG	1 cut(s)	1041
Bsp1286I	GDGCHC	2 cut(s)	841, 894
Bsp143I	GATC	3 cut(s)	125, 254, 1147
BspACI	CCGC	3 cut(s)	122, 157, 538
BspANI	GGCC	2 cut(s)	512, 744
BspCNI	CTCAG	1 cut(s)	415
BspFNI	CGCG	3 cut(s)	124, 540, 785
BspLI	GGNNCC	2 cut(s)	535, 649
BspMAI	CTGCAG	1 cut(s)	112
BspPI	GGATC	1 cut(s)	120
BspTNI	GGTCTC	2 cut(s)	348, 365
BsrDI	GCAATG	3 cut(s)	475, 690, 1070
BsrI	ACTGG	6 cut(s)	375, 393, 781, 875, 898, 1167
BssECI	CCNNGG	1 cut(s)	572
BssHII	GCGCGC	1 cut(s)	783
BssMI	GATC	3 cut(s)	125, 254, 1147
BssNI	GRCGYC	1 cut(s)	16
Bst2UI	CCWGG	4 cut(s)	359, 531, 746, 1077
Bst4CI	ACNGT	8 cut(s)	79, 559, 576, 632, 997, 1011, 1111, 1247
Bst6I	CTCTTC	1 cut(s)	932
BstACI	GRCGYC	1 cut(s)	16
BstBAI	YACGTR	2 cut(s)	325, 842
BstC8I	GCNNGC	3 cut(s)	93, 510, 785
BstDEI	CTNAG	1 cut(s)	402
BstDSI	CCRYGG	1 cut(s)	572
BstF5I	GGATG	5 cut(s)	187, 464, 568, 894, 965
BstFNI	CGCG	3 cut(s)	124, 540, 785
BstHHI	GCGC	2 cut(s)	785, 787
BstKTI	GATC	3 cut(s)	128, 257, 1150
BstMAI	GTCTC	3 cut(s)	348, 365, 652
BstMBI	GATC	3 cut(s)	125, 254, 1147
BstMCI	CGRYCG	1 cut(s)	283
BstMWI	GCNNNNNNNGC	4 cut(s)	15, 92, 101, 1172
BstNI	CCWGG	4 cut(s)	359, 531, 746, 1077
BstNSI	RCATGY	1 cut(s)	665
BstSCI	CCNGG	4 cut(s)	357, 529, 744, 1075
BstSFI	CTRYAG	2 cut(s)	108, 558
BstSLI	GKGCMC	1 cut(s)	841
BstUI	CGCG	3 cut(s)	124, 540, 785
BstV1I	GCAGC	1 cut(s)	186
BstX2I	RGATCY	1 cut(s)	1147
BstXI	CCANNNNNNTGG	1 cut(s)	579
BstYI	RGATCY	1 cut(s)	1147
BsuRI	GGCC	2 cut(s)	512, 744
BtgI	CCRYGG	1 cut(s)	572
BtgZI	GCGATG	2 cut(s)	38, 72
BtsCI	GGATG	5 cut(s)	187, 464, 568, 894, 965
BtsIMutI	CAGTG	5 cut(s)	555, 882, 993, 1007, 1174
Cac8I	GCNNGC	3 cut(s)	93, 510, 785
CfoI	GCGC	2 cut(s)	785, 787
Cfr13I	GGNCC	2 cut(s)	533, 648
CseI	GACGC	2 cut(s)	24, 1076
Csp6I	GTAC	4 cut(s)	119, 199, 295, 951
CviAII	CATG	5 cut(s)	519, 579, 601, 662, 1081
CviQI	GTAC	4 cut(s)	119, 199, 295, 951
DdeI	CTNAG	1 cut(s)	402
DpnI	GATC	3 cut(s)	127, 256, 1149
DpnII	GATC	3 cut(s)	125, 254, 1147
DraI	TTTAAA	1 cut(s)	432
EaeI	YGGCCR	1 cut(s)	510
Eam1104I	CTCTTC	1 cut(s)	932
EarI	CTCTTC	1 cut(s)	932
Eco24I	GRGCYC	1 cut(s)	894
Eco31I	GGTCTC	2 cut(s)	348, 365
Eco47I	GGWCC	2 cut(s)	533, 648
Eco72I	CACGTG	1 cut(s)	842
Eco88I	CYCGRG	1 cut(s)	1041
EcoO109I	RGGNCCY	1 cut(s)	533
EcoRII	CCWGG	4 cut(s)	357, 529, 744, 1075
EcoT22I	ATGCAT	1 cut(s)	464
EcoT38I	GRGCYC	1 cut(s)	894
FaeI	CATG	5 cut(s)	522, 582, 604, 665, 1084
FalI	AAGNNNNNCTT	2 cut(s)	438, 470
FaqI	GGGAC	2 cut(s)	405, 661
FatI	CATG	5 cut(s)	518, 578, 600, 661, 1080
FauI	CCCGC	1 cut(s)	545
FblI	GTMKAC	2 cut(s)	13, 327
Fnu4HI	GCNGC	2 cut(s)	157, 175
FokI	GGATG	5 cut(s)	174, 471, 555, 881, 972
FriOI	GRGCYC	1 cut(s)	894
Fsp4HI	GCNGC	2 cut(s)	157, 175
FspBI	CTAG	2 cut(s)	153, 503
GlaI	GCGC	2 cut(s)	784, 786
GluI	GCNGC	2 cut(s)	157, 175
GsuI	CTGGAG	1 cut(s)	767
HaeIII	GGCC	2 cut(s)	512, 744
HgaI	GACGC	2 cut(s)	24, 1076
HhaI	GCGC	2 cut(s)	785, 787
Hin1I	GRCGYC	1 cut(s)	16
Hin1II	CATG	5 cut(s)	522, 582, 604, 665, 1084
Hin6I	GCGC	2 cut(s)	783, 785
HinP1I	GCGC	2 cut(s)	783, 785
HincII	GTYRAC	2 cut(s)	14, 486
HindII	GTYRAC	2 cut(s)	14, 486
HindIII	AAGCTT	1 cut(s)	102
HinfI	GANTC	3 cut(s)	226, 482, 1039
HphI	GGTGA	4 cut(s)	21, 235, 620, 792
Hpy166II	GTNNAC	6 cut(s)	14, 328, 409, 452, 486, 923
Hpy188III	TCNNGA	2 cut(s)	318, 503
Hpy8I	GTNNAC	6 cut(s)	14, 328, 409, 452, 486, 923
Hpy99I	CGWCG	4 cut(s)	15, 18, 206, 958
HpyAV	CCTTC	3 cut(s)	42, 51, 1203
HpyCH4III	ACNGT	8 cut(s)	79, 559, 576, 632, 997, 1011, 1111, 1247
HpyCH4IV	ACGT	4 cut(s)	138, 324, 841, 1186
HpyF10VI	GCNNNNNNNGC	4 cut(s)	15, 92, 101, 1172
HpyF3I	CTNAG	1 cut(s)	402
HpySE526I	ACGT	4 cut(s)	138, 324, 841, 1186
Hsp92I	GRCGYC	1 cut(s)	16
Hsp92II	CATG	5 cut(s)	522, 582, 604, 665, 1084
HspAI	GCGC	2 cut(s)	783, 785
Kzo9I	GATC	3 cut(s)	125, 254, 1147
LmnI	GCTCC	3 cut(s)	583, 780, 1207
Lsp1109I	GCAGC	1 cut(s)	186
LweI	GCATC	5 cut(s)	61, 95, 196, 449, 471
MaeI	CTAG	2 cut(s)	153, 503
MaeII	ACGT	4 cut(s)	138, 324, 841, 1186
MaeIII	GTNAC	4 cut(s)	472, 664, 798, 1115
MalI	GATC	3 cut(s)	127, 256, 1149
MboI	GATC	3 cut(s)	125, 254, 1147
MboII	GAAGA	4 cut(s)	27, 36, 949, 1157
MfeI	CAATTG	1 cut(s)	130
MflI	RGATCY	1 cut(s)	1147
MhlI	GDGCHC	2 cut(s)	841, 894
MlsI	TGGCCA	1 cut(s)	512
MluCI	AATT	5 cut(s)	130, 364, 755, 1086, 1251
MluNI	TGGCCA	1 cut(s)	512
MlyI	GAGTC	2 cut(s)	491, 1033
MmeI	TCCRAC	1 cut(s)	1232
MnlI	CCTC	7 cut(s)	37, 58, 200, 874, 1016, 1186, 1215
Mox20I	TGGCCA	1 cut(s)	512
Mph1103I	ATGCAT	1 cut(s)	464
MscI	TGGCCA	1 cut(s)	512
MseI	TTAA	6 cut(s)	431, 546, 723, 753, 812, 1152
MslI	CAYNNNNRTG	2 cut(s)	577, 704
Msp20I	TGGCCA	1 cut(s)	512
MspA1I	CMGCKG	1 cut(s)	177
MspR9I	CCNGG	4 cut(s)	359, 531, 746, 1077
MunI	CAATTG	1 cut(s)	130
MvaI	CCWGG	4 cut(s)	359, 531, 746, 1077
MvnI	CGCG	3 cut(s)	124, 540, 785
MwoI	GCNNNNNNNGC	4 cut(s)	15, 92, 101, 1172
NdeII	GATC	3 cut(s)	125, 254, 1147
NlaIII	CATG	5 cut(s)	522, 582, 604, 665, 1084
NlaIV	GGNNCC	2 cut(s)	535, 649
NmuCI	GTSAC	3 cut(s)	472, 798, 1115
NsiI	ATGCAT	1 cut(s)	464
NspI	RCATGY	1 cut(s)	665
OliI	CACNNNNGTG	1 cut(s)	704
PaeR7I	CTCGAG	1 cut(s)	1041
PauI	GCGCGC	1 cut(s)	783
PciI	ACATGT	1 cut(s)	661
PfeI	GAWTC	1 cut(s)	226
PflMI	CCANNNNNTGG	1 cut(s)	740
PkrI	GCNGC	2 cut(s)	158, 176
PleI	GAGTC	2 cut(s)	490, 1033
PmaCI	CACGTG	1 cut(s)	842
PmlI	CACGTG	1 cut(s)	842
PpsI	GAGTC	2 cut(s)	490, 1033
Ppu21I	YACGTR	2 cut(s)	325, 842
PpuMI	RGGWCCY	1 cut(s)	533
PscI	ACATGT	1 cut(s)	661
Psp1406I	AACGTT	1 cut(s)	1186
Psp5II	RGGWCCY	1 cut(s)	533
Psp6I	CCWGG	4 cut(s)	357, 529, 744, 1075
PspCI	CACGTG	1 cut(s)	842
PspGI	CCWGG	4 cut(s)	357, 529, 744, 1075
PspN4I	GGNNCC	2 cut(s)	535, 649
PspPI	GGNCC	2 cut(s)	533, 648
PspPPI	RGGWCCY	1 cut(s)	533
PspXI	VCTCGAGB	1 cut(s)	1041
PsrI	GAACNNNNNNTAC	2 cut(s)	318, 350
PstI	CTGCAG	1 cut(s)	112
PsuI	RGATCY	1 cut(s)	1147
PteI	GCGCGC	1 cut(s)	783
PvuII	CAGCTG	1 cut(s)	177
RsaI	GTAC	4 cut(s)	120, 200, 296, 952
RsaNI	GTAC	4 cut(s)	119, 199, 295, 951
RseI	CAYNNNNRTG	2 cut(s)	577, 704
SalI	GTCGAC	1 cut(s)	12
SaqAI	TTAA	6 cut(s)	431, 546, 723, 753, 812, 1152
SatI	GCNGC	2 cut(s)	157, 175
Sau3AI	GATC	3 cut(s)	125, 254, 1147
Sau96I	GGNCC	2 cut(s)	533, 648
SchI	GAGTC	2 cut(s)	491, 1033
ScrFI	CCNGG	4 cut(s)	359, 531, 746, 1077
SduI	GDGCHC	2 cut(s)	841, 894
SfaNI	GCATC	5 cut(s)	61, 95, 196, 449, 471
SfcI	CTRYAG	2 cut(s)	108, 558
Sfr274I	CTCGAG	1 cut(s)	1041
SgrDI	CGTCGACG	1 cut(s)	12
SinI	GGWCC	2 cut(s)	533, 648
SlaI	CTCGAG	1 cut(s)	1041
SmiMI	CAYNNNNRTG	2 cut(s)	577, 704
SmlI	CTYRAG	1 cut(s)	1041
SmoI	CTYRAG	1 cut(s)	1041
Sse9I	AATT	5 cut(s)	130, 364, 755, 1086, 1251
SsiI	CCGC	3 cut(s)	122, 157, 538
SspMI	CTAG	2 cut(s)	153, 503
StyD4I	CCNGG	4 cut(s)	357, 529, 744, 1075
TaaI	ACNGT	8 cut(s)	79, 559, 576, 632, 997, 1011, 1111, 1247
TaiI	ACGT	4 cut(s)	141, 327, 844, 1189
TaqI	TCGA	6 cut(s)	13, 144, 165, 283, 1042, 1223
TaqII	GACCGA	2 cut(s)	269, 665
TasI	AATT	5 cut(s)	130, 364, 755, 1086, 1251
TatI	WGTACW	1 cut(s)	294
TauI	GCSGC	1 cut(s)	159
TfiI	GAWTC	1 cut(s)	226
Tru1I	TTAA	6 cut(s)	431, 546, 723, 753, 812, 1152
Tru9I	TTAA	6 cut(s)	431, 546, 723, 753, 812, 1152
TscAI	CASTG	5 cut(s)	562, 882, 1000, 1014, 1174
TseFI	GTSAC	3 cut(s)	472, 798, 1115
TseI	GCWGC	1 cut(s)	174
Tsp45I	GTSAC	3 cut(s)	472, 798, 1115
TspDTI	ATGAA	3 cut(s)	633, 870, 1248
TspGWI	ACGGA	3 cut(s)	180, 219, 840
TspRI	CASTG	5 cut(s)	562, 882, 1000, 1014, 1174
Van91I	CCANNNNNTGG	1 cut(s)	740
VpaK11BI	GGWCC	2 cut(s)	533, 648
XbaI	TCTAGA	1 cut(s)	502
XceI	RCATGY	1 cut(s)	665
XhoI	CTCGAG	1 cut(s)	1041
XmiI	GTMKAC	2 cut(s)	13, 327
XspI	CTAG	2 cut(s)	153, 503
Zsp2I	ATGCAT	1 cut(s)	464

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.