pycom15g24590

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003455
Physical Location & Seq
Reverse (-)
18793154 .. 18793450
297 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 297 bp
ATGGCCATAATGTGTTGTGTGCAGGTGCAGCCTAACCAGGAGATGACGGTGGAAGAGTTCAAAGCGTGGATTTACGGATTTGACAATGATCACAACGGGCAGATTAGCCGTGAGGAACTGAAAGAGGCTCTTCGCAGCCTGAGGGTTTGGTTCGGGTGGTGGAGAGCTCGCCAAGCAATTAAGTCATCGGATTTCAACGGTAGTGGCCAAATCGACAACCCCAAAGAGTTTGAGAAGCTTGTTAACTATGCACAGCAACGTCTAGGCATGAAAATTCATGAAAATAATGATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5

Protein Analysis

99

Amino Acids

11.64

Weight (kDa)

5.64

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 13
AcoI YGGCCR 2 cut(s) 3, 205
AcsI RAATTY 1 cut(s) 273
AgsI TTSAA 2 cut(s) 61, 196
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 2 cut(s) 167, 238
AluI AGCT 2 cut(s) 167, 238
Alw21I GWGCWC 1 cut(s) 169
AoxI GGCC 2 cut(s) 3, 205
ApeKI GCWGC 2 cut(s) 28, 135
ApoI RAATTY 1 cut(s) 273
AxyI CCTNAGG 1 cut(s) 140
BalI TGGCCA 2 cut(s) 5, 207
BanII GRGCYC 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 169
BbvI GCAGC 2 cut(s) 40, 147
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 1 cut(s) 38
BclI TGATCA 1 cut(s) 88
BfaI CTAG 1 cut(s) 263
BfuAI ACCTGC 1 cut(s) 13
BisI GCNGC 2 cut(s) 29, 136
BlsI GCNGC 2 cut(s) 30, 137
Bme1390I CCNGG 1 cut(s) 38
BmrFI CCNGG 1 cut(s) 38
Bse21I CCTNAGG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 38
BseMII CTCAG 1 cut(s) 131
BseXI GCAGC 2 cut(s) 40, 147
BsgI GTGCAG 2 cut(s) 41, 47
BshFI GGCC 2 cut(s) 5, 207
BsiHKAI GWGCWC 1 cut(s) 169
BsnI GGCC 2 cut(s) 5, 207
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 1 cut(s) 88
BspANI GGCC 2 cut(s) 5, 207
BspCNI CTCAG 1 cut(s) 132
BspHI TCATGA 1 cut(s) 277
BspMI ACCTGC 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 135
BssMI GATC 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 2 cut(s) 49, 200
Bst6I CTCTTC 2 cut(s) 48, 135
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 1 cut(s) 140
BstKTI GATC 1 cut(s) 91
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 2 cut(s) 28, 173
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 36
BstV1I GCAGC 2 cut(s) 40, 147
Bsu36I CCTNAGG 1 cut(s) 140
BsuRI GGCC 2 cut(s) 5, 207
BveI ACCTGC 1 cut(s) 13
Cac8I GCNNGC 1 cut(s) 169
CciI TCATGA 1 cut(s) 277
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviAII CATG 2 cut(s) 268, 278
CviJI RGCY 8 cut(s) 5, 31, 108, 128, 138, 167, 207, 238
CviKI_1 RGCY 8 cut(s) 5, 31, 108, 128, 138, 167, 207, 238
DdeI CTNAG 1 cut(s) 140
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
EaeI YGGCCR 2 cut(s) 3, 205
Eam1104I CTCTTC 2 cut(s) 48, 135
EarI CTCTTC 2 cut(s) 48, 135
Ecl136II GAGCTC 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 169
Eco53kI GAGCTC 1 cut(s) 167
Eco81I CCTNAGG 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 169
FaeI CATG 2 cut(s) 271, 281
FaiI YATR 4 cut(s) 8, 249, 269, 279
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FatI CATG 2 cut(s) 267, 277
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 29, 136
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 2 cut(s) 29, 136
FspBI CTAG 1 cut(s) 263
GluI GCNGC 2 cut(s) 29, 136
HaeIII GGCC 2 cut(s) 5, 207
Hin1II CATG 2 cut(s) 271, 281
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 236
HpaI GTTAAC 1 cut(s) 244
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 1 cut(s) 278
Hpy8I GTNNAC 1 cut(s) 244
HpyCH4III ACNGT 2 cut(s) 49, 200
HpyCH4IV ACGT 1 cut(s) 259
HpyCH4V TGCA 3 cut(s) 22, 28, 251
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 173
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 259
Hsp92II CATG 2 cut(s) 271, 281
Ksp22I TGATCA 1 cut(s) 88
KspAI GTTAAC 1 cut(s) 244
Kzo9I GATC 1 cut(s) 88
LguI GCTCTTC 1 cut(s) 135
LpnPI CCDG 4 cut(s) 8, 23, 50, 152
Lsp1109I GCAGC 2 cut(s) 40, 147
MaeI CTAG 1 cut(s) 263
MaeII ACGT 1 cut(s) 259
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 2 cut(s) 65, 122
MhlI GDGCHC 1 cut(s) 169
MlsI TGGCCA 2 cut(s) 5, 207
MluCI AATT 2 cut(s) 177, 273
MluNI TGGCCA 2 cut(s) 5, 207
MnlI CCTC 3 cut(s) 106, 118, 135
Mox20I TGGCCA 2 cut(s) 5, 207
MscI TGGCCA 2 cut(s) 5, 207
MseI TTAA 2 cut(s) 180, 243
Msp20I TGGCCA 2 cut(s) 5, 207
MspR9I CCNGG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 2 cut(s) 28, 173
NdeII GATC 1 cut(s) 88
NlaIII CATG 2 cut(s) 271, 281
PagI TCATGA 1 cut(s) 277
PaqCI CACCTGC 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 135
PkrI GCNGC 2 cut(s) 30, 137
Psp124BI GAGCTC 1 cut(s) 169
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
SacI GAGCTC 1 cut(s) 169
SapI GCTCTTC 1 cut(s) 135
SaqAI TTAA 2 cut(s) 180, 243
SatI GCNGC 2 cut(s) 29, 136
Sau3AI GATC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 1 cut(s) 169
SetI ASST 4 cut(s) 27, 169, 240, 262
Sse9I AATT 2 cut(s) 177, 273
SspMI CTAG 1 cut(s) 263
SstI GAGCTC 1 cut(s) 169
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 2 cut(s) 49, 200
TaiI ACGT 1 cut(s) 262
TaqI TCGA 1 cut(s) 213
TasI AATT 2 cut(s) 177, 273
Tru1I TTAA 2 cut(s) 180, 243
Tru9I TTAA 2 cut(s) 180, 243
TseI GCWGC 2 cut(s) 28, 135
TspDTI ATGAA 3 cut(s) 266, 284, 294
TspGWI ACGGA 1 cut(s) 90
XapI RAATTY 1 cut(s) 273
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.