pycom15g30230

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002954
Physical Location & Seq
Forward (+)
27588642 .. 27593036
4395 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1287 bp
ATGTCGGTAACAGCAGGTGTAAGCGATACTGTTATTGCAGTAAGGGATAAGCTCAGAGGTAAAATTGGGCAAACTAAGGTTAAAAGGTATTGGCCTGGAAAAGCTCCGGAGTGGGCAGATGATGCCGACGAAGATGGGGAGTTTAAGATGTCCAAGGCAGCTGCTTTTCCCACTCATGATTCTGACGTTGTTAGGAAAGATGATCCTAGACTGCGTCGTTTGGCTGAGAGCAGGATAGATAATCGTGAGGATGTTAGAGCTGACCATAGGCGTATTCGGCAAGCTGAGATTGTTTCAACAATTGAAGAGGAGGCCAGGAGGCAGGAAGGGTTGGAAGCAGAGGAAGAGGATGCAGATGCCCAGGAAGAAAGAAGAAGAAAGATTAGGGAGAGGTTGCTTCAGAGGGAGCAAGAAGAAGAAGAACTACTTCCCTCCGAAGATGAGGAAGAGGTACAGGAAGAGGAGGAAGAGGAATCTGAGTATGACACTGACTCGGACGAAGAGCTTACTGGTATGGTGATGCTAAAGCCTGTCTTTGTTCCCAAGTCAGAGAGAGATACTATTGCTGAGCGTGAGCGACTTGAGGCCGAAGAACGGGCCCTTGAGGAATCAAGGAAGAAGAAATTGGAGGAAAGGAAGAGAGAGACAAAGCAGATTGTGGTTGAGGAGATTCGGAAGGATGAAGAGATTCAGAAGGGTTTGGAATTGGAAGCAAATATTGAAGATATTGATACTGATGATGAACTAAACGAGGCAGAGGAGTATGAAGCTTGGAAGGCAAGAGAGATTGCCCGAATCAAAAGGGATAGGGAGGATAGGGAAGCAATGTTAAAGGAAAAGGAAGAGATTGAAAGGATCAGAAACATGACAGAGGAAGAGAGGAGGGAGTGGGAGAGAAAGAATCCAAGACCTGCACCACGACCAAAGCAGAAGTGGAGGTTCATGCAGAAATATTACCACAAAGGTGCTTTCTTCCAGTCAGAAGCTGATGACTATGCGGCAACGGCTGGAACAGATGGAATTTATGCACGTGATGTCTCTTCCCCTACTGGAGAAGACAAGATGGACAAAACGATATTGCCCAAGGTCATGCAGGTCAAACATTTTGGTCGTAGTGGAAGGACGAAATGGACCCATCTTGTCAATGAGGATACCACTGATTGGAACAATCCGTGGACATACAACGATCCTCTTCGGGCAAAGTATAATGCTAAAATGGCAGGAATGAATGCACCTATTTCAAAACCCAAAGGAAGCAAGAAATTGAAGGACTGGGAGTCTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

429

Amino Acids

50.56

Weight (kDa)

4.99

Isoelectric Point (pI)

65.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 164 - 385 2e-76 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5
Acc36I ACCTGC 3 cut(s) 5, 919, 1084
AccB7I CCANNNNNTGG 1 cut(s) 1161
AccIII TCCGGA 1 cut(s) 106
AciI CCGC 1 cut(s) 998
AclWI GGATC 3 cut(s) 197, 863, 1181
AcsI RAATTY 1 cut(s) 1020
AcuI CTGAAG 1 cut(s) 383
AcvI CACGTG 1 cut(s) 1031
AfaI GTAC 1 cut(s) 453
AfiI CCNNNNNNNGG 2 cut(s) 594, 1161
AgsI TTSAA 6 cut(s) 297, 305, 722, 851, 1242, 1267
AhdI GACNNNNNGTC 1 cut(s) 1276
AjnI CCWGG 3 cut(s) 94, 314, 360
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AleI CACNNNNGTG 1 cut(s) 963
AluBI AGCT 8 cut(s) 52, 104, 161, 260, 284, 505, 770, 986
AluI AGCT 8 cut(s) 52, 104, 161, 260, 284, 505, 770, 986
Alw26I GTCTC 2 cut(s) 638, 1042
AlwI GGATC 3 cut(s) 197, 863, 1181
AlwNI CAGNNNCTG 1 cut(s) 986
Aor13HI TCCGGA 1 cut(s) 106
AoxI GGCC 4 cut(s) 92, 312, 585, 597
ApaI GGGCCC 1 cut(s) 601
ApeKI GCWGC 2 cut(s) 158, 161
ApoI RAATTY 1 cut(s) 1020
Asp700I GAANNNNTTC 2 cut(s) 426, 687
AspS9I GGNCC 3 cut(s) 597, 598, 1131
AsuHPI GGTGA 1 cut(s) 529
AvaII GGWCC 1 cut(s) 1131
BaeGI GKGCMC 1 cut(s) 601
BanII GRGCYC 1 cut(s) 601
BarI GAAGNNNNNNTAC 2 cut(s) 408, 440
BbrPI CACGTG 1 cut(s) 1031
BbsI GAAGAC 1 cut(s) 1062
BbvI GCAGC 2 cut(s) 148, 170
BccI CCATC 4 cut(s) 128, 1010, 1057, 1143
BceAI ACGGC 1 cut(s) 1020
BciT130I CCWGG 3 cut(s) 96, 316, 362
BciVI GTATCC 1 cut(s) 1144
BcoDI GTCTC 2 cut(s) 638, 1042
BfaI CTAG 1 cut(s) 207
BfuAI ACCTGC 3 cut(s) 5, 919, 1084
BfuI GTATCC 1 cut(s) 1144
BisI GCNGC 3 cut(s) 159, 162, 999
BlpI GCTNAGC 1 cut(s) 567
BlsI GCNGC 3 cut(s) 160, 163, 1000
Bme1390I CCNGG 3 cut(s) 96, 316, 362
Bme18I GGWCC 1 cut(s) 1131
BmeRI GACNNNNNGTC 1 cut(s) 1276
BmgT120I GGNCC 3 cut(s) 597, 598, 1131
BmiI GGNNCC 2 cut(s) 599, 1133
BmrFI CCNGG 3 cut(s) 96, 316, 362
BmrI ACTGGG 1 cut(s) 1282
BmsI GCATC 4 cut(s) 112, 340, 346, 510
BmuI ACTGGG 1 cut(s) 1282
BpiI GAAGAC 1 cut(s) 1062
BpmI CTGGAG 1 cut(s) 1071
Bpu1102I GCTNAGC 1 cut(s) 567
BpuEI CTTGAG 2 cut(s) 602, 623
BsaAI YACGTR 1 cut(s) 1031
BsaJI CCNNGG 4 cut(s) 153, 360, 1083, 1172
BsaWI WCCGGW 1 cut(s) 106
Bsc4I CCNNNNNNNGG 2 cut(s) 594, 1161
Bse1I ACTGG 4 cut(s) 514, 976, 1054, 1277
Bse3DI GCAATG 1 cut(s) 831
BseAI TCCGGA 1 cut(s) 106
BseBI CCWGG 3 cut(s) 96, 316, 362
BseDI CCNNGG 4 cut(s) 153, 360, 1083, 1172
BseGI GGATG 3 cut(s) 256, 355, 685
BseLI CCNNNNNNNGG 2 cut(s) 594, 1161
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 5 cut(s) 67, 216, 276, 468, 558
BseNI ACTGG 4 cut(s) 514, 976, 1054, 1277
BseRI GAGGAG 5 cut(s) 323, 476, 680, 773, 895
BseSI GKGCMC 1 cut(s) 601
BseXI GCAGC 2 cut(s) 148, 170
BsgI GTGCAG 1 cut(s) 897
BshFI GGCC 4 cut(s) 94, 314, 587, 599
BsiSI CCGG 1 cut(s) 107
BslI CCNNNNNNNGG 2 cut(s) 594, 1161
BsmAI GTCTC 2 cut(s) 638, 1042
BsmI GAATGC 1 cut(s) 1234
BsnI GGCC 4 cut(s) 94, 314, 587, 599
Bsp120I GGGCCC 1 cut(s) 597
Bsp1286I GDGCHC 1 cut(s) 601
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 3 cut(s) 202, 855, 1186
Bsp1720I GCTNAGC 1 cut(s) 567
BspACI CCGC 1 cut(s) 998
BspANI GGCC 4 cut(s) 94, 314, 587, 599
BspCNI CTCAG 5 cut(s) 66, 217, 277, 469, 559
BspEI TCCGGA 1 cut(s) 106
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 599, 1133
BspMI ACCTGC 3 cut(s) 5, 919, 1084
BspPI GGATC 3 cut(s) 197, 863, 1181
BspQI GCTCTTC 1 cut(s) 495
BsrDI GCAATG 1 cut(s) 831
BsrI ACTGG 4 cut(s) 514, 976, 1054, 1277
BssECI CCNNGG 4 cut(s) 153, 360, 1083, 1172
BssMI GATC 3 cut(s) 202, 855, 1186
BssT1I CCWWGG 2 cut(s) 153, 1083
Bst2UI CCWGG 3 cut(s) 96, 316, 362
Bst4CI ACNGT 1 cut(s) 31
BstAPI GCANNNNNTGC 1 cut(s) 122
BstBAI YACGTR 1 cut(s) 1031
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 6 cut(s) 53, 75, 225, 285, 477, 567
BstDSI CCRYGG 1 cut(s) 1172
BstF5I GGATG 3 cut(s) 256, 355, 685
BstKTI GATC 3 cut(s) 205, 858, 1189
BstMAI GTCTC 2 cut(s) 638, 1042
BstMBI GATC 3 cut(s) 202, 855, 1186
BstMWI GCNNNNNNNGC 5 cut(s) 122, 277, 776, 1004, 1217
BstNI CCWGG 3 cut(s) 96, 316, 362
BstSCI CCNGG 3 cut(s) 94, 314, 360
BstSLI GKGCMC 1 cut(s) 601
BstV1I GCAGC 2 cut(s) 148, 170
BstV2I GAAGAC 1 cut(s) 1062
BsuI GTATCC 1 cut(s) 1144
BsuRI GGCC 4 cut(s) 94, 314, 587, 599
BtgI CCRYGG 1 cut(s) 1172
BtsCI GGATG 3 cut(s) 256, 355, 685
BtsIMutI CAGTG 2 cut(s) 486, 1155
BveI ACCTGC 3 cut(s) 5, 919, 1084
Cac8I GCNNGC 1 cut(s) 282
CaiI CAGNNNCTG 1 cut(s) 986
CciI TCATGA 1 cut(s) 175
Cfr13I GGNCC 3 cut(s) 597, 598, 1131
CseI GACGC 1 cut(s) 203
Csp6I GTAC 1 cut(s) 452
CviAII CATG 4 cut(s) 176, 865, 943, 1090
CviQI GTAC 1 cut(s) 452
DdeI CTNAG 6 cut(s) 53, 75, 225, 285, 477, 567
DpnI GATC 3 cut(s) 204, 857, 1188
DpnII GATC 3 cut(s) 202, 855, 1186
DriI GACNNNNNGTC 1 cut(s) 1276
Eam1105I GACNNNNNGTC 1 cut(s) 1276
Eco130I CCWWGG 2 cut(s) 153, 1083
Eco24I GRGCYC 1 cut(s) 601
Eco47I GGWCC 1 cut(s) 1131
Eco57I CTGAAG 1 cut(s) 383
Eco72I CACGTG 1 cut(s) 1031
EcoO109I RGGNCCY 1 cut(s) 598
EcoRII CCWGG 3 cut(s) 94, 314, 360
EcoT14I CCWWGG 2 cut(s) 153, 1083
EcoT38I GRGCYC 1 cut(s) 601
ErhI CCWWGG 2 cut(s) 153, 1083
FaeI CATG 4 cut(s) 179, 868, 946, 1093
FalI AAGNNNNNCTT 4 cut(s) 411, 443, 518, 550
FatI CATG 4 cut(s) 175, 864, 942, 1089
Fnu4HI GCNGC 3 cut(s) 159, 162, 999
FokI GGATG 3 cut(s) 263, 362, 692
FriOI GRGCYC 1 cut(s) 601
Fsp4HI GCNGC 3 cut(s) 159, 162, 999
FspBI CTAG 1 cut(s) 207
GluI GCNGC 3 cut(s) 159, 162, 999
GsuI CTGGAG 1 cut(s) 1071
HaeIII GGCC 4 cut(s) 94, 314, 587, 599
HapII CCGG 1 cut(s) 107
HgaI GACGC 1 cut(s) 203
Hin1II CATG 4 cut(s) 179, 868, 946, 1093
HindIII AAGCTT 1 cut(s) 768
HinfI GANTC 9 cut(s) 179, 473, 491, 608, 670, 688, 795, 901, 1277
HpaII CCGG 1 cut(s) 107
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 1 cut(s) 1176
Hpy188III TCNNGA 3 cut(s) 107, 176, 245
Hpy8I GTNNAC 1 cut(s) 1176
Hpy99I CGWCG 2 cut(s) 131, 219
HpyAV CCTTC 6 cut(s) 320, 670, 688, 769, 1113, 1261
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 2 cut(s) 186, 1030
HpyCH4V TGCA 7 cut(s) 38, 353, 914, 946, 1028, 1093, 1232
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 277, 776, 1004, 1217
HpyF3I CTNAG 6 cut(s) 53, 75, 225, 285, 477, 567
HpySE526I ACGT 2 cut(s) 186, 1030
Hsp92II CATG 4 cut(s) 179, 868, 946, 1093
Kpn2I TCCGGA 1 cut(s) 106
Kzo9I GATC 3 cut(s) 202, 855, 1186
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 2 cut(s) 109, 406
Lsp1109I GCAGC 2 cut(s) 148, 170
LweI GCATC 4 cut(s) 112, 340, 346, 510
MaeI CTAG 1 cut(s) 207
MaeII ACGT 2 cut(s) 186, 1030
MaeIII GTNAC 1 cut(s) 7
MalI GATC 3 cut(s) 204, 857, 1188
MboI GATC 3 cut(s) 202, 855, 1186
MfeI CAATTG 1 cut(s) 300
MhlI GDGCHC 1 cut(s) 601
MluCI AATT 6 cut(s) 63, 300, 623, 704, 1020, 1262
MlyI GAGTC 2 cut(s) 485, 1286
MmeI TCCRAC 1 cut(s) 312
MroI TCCGGA 1 cut(s) 106
MroXI GAANNNNTTC 2 cut(s) 426, 687
MseI TTAA 3 cut(s) 81, 144, 830
MslI CAYNNNNRTG 1 cut(s) 963
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 3 cut(s) 96, 316, 362
MunI CAATTG 1 cut(s) 300
Mva1269I GAATGC 1 cut(s) 1234
MvaI CCWGG 3 cut(s) 96, 316, 362
MwoI GCNNNNNNNGC 5 cut(s) 122, 277, 776, 1004, 1217
NdeII GATC 3 cut(s) 202, 855, 1186
NlaIII CATG 4 cut(s) 179, 868, 946, 1093
NlaIV GGNNCC 2 cut(s) 599, 1133
OliI CACNNNNGTG 1 cut(s) 963
PagI TCATGA 1 cut(s) 175
PaqCI CACCTGC 1 cut(s) 5
PciSI GCTCTTC 1 cut(s) 495
PctI GAATGC 1 cut(s) 1234
PdmI GAANNNNTTC 2 cut(s) 426, 687
PfeI GAWTC 7 cut(s) 179, 473, 608, 670, 688, 795, 901
PflFI GACNNNGTC 1 cut(s) 213
PflMI CCANNNNNTGG 1 cut(s) 1161
PkrI GCNGC 3 cut(s) 160, 163, 1000
PleI GAGTC 2 cut(s) 485, 1285
PmaCI CACGTG 1 cut(s) 1031
PmlI CACGTG 1 cut(s) 1031
PpsI GAGTC 2 cut(s) 485, 1285
Ppu21I YACGTR 1 cut(s) 1031
Psp6I CCWGG 3 cut(s) 94, 314, 360
PspCI CACGTG 1 cut(s) 1031
PspGI CCWGG 3 cut(s) 94, 314, 360
PspN4I GGNNCC 2 cut(s) 599, 1133
PspOMI GGGCCC 1 cut(s) 597
PspPI GGNCC 3 cut(s) 597, 598, 1131
PstNI CAGNNNCTG 1 cut(s) 986
PsyI GACNNNGTC 1 cut(s) 213
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 1 cut(s) 453
RsaNI GTAC 1 cut(s) 452
RseI CAYNNNNRTG 1 cut(s) 963
SapI GCTCTTC 1 cut(s) 495
SaqAI TTAA 3 cut(s) 81, 144, 830
SatI GCNGC 3 cut(s) 159, 162, 999
Sau3AI GATC 3 cut(s) 202, 855, 1186
Sau96I GGNCC 3 cut(s) 597, 598, 1131
SchI GAGTC 2 cut(s) 485, 1286
ScrFI CCNGG 3 cut(s) 96, 316, 362
SduI GDGCHC 1 cut(s) 601
SfaNI GCATC 4 cut(s) 112, 340, 346, 510
SinI GGWCC 1 cut(s) 1131
SmiMI CAYNNNNRTG 1 cut(s) 963
SmlI CTYRAG 2 cut(s) 581, 602
SmoI CTYRAG 2 cut(s) 581, 602
Sse9I AATT 6 cut(s) 63, 300, 623, 704, 1020, 1262
SsiI CCGC 1 cut(s) 998
SspI AATATT 2 cut(s) 718, 953
SspMI CTAG 1 cut(s) 207
StyD4I CCNGG 3 cut(s) 94, 314, 360
StyI CCWWGG 2 cut(s) 153, 1083
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 2 cut(s) 189, 1033
TasI AATT 6 cut(s) 63, 300, 623, 704, 1020, 1262
TauI GCSGC 1 cut(s) 1001
TfiI GAWTC 7 cut(s) 179, 473, 608, 670, 688, 795, 901
Tru1I TTAA 3 cut(s) 81, 144, 830
Tru9I TTAA 3 cut(s) 81, 144, 830
TscAI CASTG 2 cut(s) 493, 1162
TseI GCWGC 2 cut(s) 158, 161
TspDTI ATGAA 5 cut(s) 696, 756, 780, 931, 1241
TspGWI ACGGA 1 cut(s) 1161
TspRI CASTG 2 cut(s) 493, 1162
Tth111I GACNNNGTC 1 cut(s) 213
Van91I CCANNNNNTGG 1 cut(s) 1161
VpaK11BI GGWCC 1 cut(s) 1131
XapI RAATTY 1 cut(s) 1020
XcmI CCANNNNNNNNNTGG 1 cut(s) 930
XmnI GAANNNNTTC 2 cut(s) 426, 687
XspI CTAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.