pycom15g31770

Cold shock protein domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006037
Physical Location & Seq
Forward (+)
30478704 .. 30480094
1391 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g31770.2

Sequence Viewer

Length: 693 bp
ATGGCTGACGCCGCGAAATCCACGGGCACGGTCAAGTGGTTCAGCGCGCATAAGGGGTTCGGCTTCATAGCTCCCGACGACGGCGGTGAGGATCTCTTCGTCCACCAGACCTCGATCCTATCCGACGGCTTCCGGACCCTCTCGGAGGGCCAGACCGTCGAGTTCCTTGTAGATTTCAGTGAGGACGGACGCACCAAAGCCATCGACGTCGTTGGAAATATCACTCGATCCCGCCGTCCCGGCTTTGACCGCGGCCGCGGTCGTGGTGGATATGGAGGGAGAGGCGGCGGAGGCGGTTATGGAAGGTTTGGTGGAGACCCTGGAGGGTATAATGTAGCGAGAGGCGGCGGAAGGCGTGGCGGCTACGGCGGAGGTCGTGGGGGCGGCGGCGGTGAGTGCTACAACTGCGGTGGTATTGGACATTTGGCAAGGGACTGTACTCAGAGTGGTGGTGTGACTGGCGTCCCGAAATTTGGCGGCGGTGGACGCGGTGGTCGGGGATATGGTGGTAGAGGCGGCGGCAGGCGTGGTGGTGAATGTTATAATTGTGGAGAGGAAGGGCATTTGTGTTGTGGATGTTGTGGTCGCTGCTCAAATTTTGTCGTTGGAAGACGATTTTATGGTTTAGCTGTTCTATTAAGTTATCATCAATTGAATGGTAGCAGAAGGGCAGACCAACAACATGCTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CSD zf-CCHC

Protein Analysis

231

Amino Acids

23.66

Weight (kDa)

9.3

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 543
AasI GACNNNNNNGTC 1 cut(s) 492
AatII GACGTC 1 cut(s) 210
AccII CGCG 5 cut(s) 14, 47, 252, 258, 489
AccIII TCCGGA 1 cut(s) 132
AclWI GGATC 3 cut(s) 99, 109, 222
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 2 cut(s) 470, 595
AcyI GRCGYC 3 cut(s) 9, 207, 462
AfaI GTAC 1 cut(s) 439
AfiI CCNNNNNNNGG 3 cut(s) 80, 145, 473
AgsI TTSAA 1 cut(s) 655
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 2 cut(s) 71, 629
AluI AGCT 2 cut(s) 71, 629
Alw26I GTCTC 1 cut(s) 309
AlwI GGATC 3 cut(s) 99, 109, 222
Aor13HI TCCGGA 1 cut(s) 132
AoxI GGCC 2 cut(s) 148, 253
ApeKI GCWGC 1 cut(s) 588
ApoI RAATTY 2 cut(s) 470, 595
AspLEI GCGC 2 cut(s) 47, 49
AspS9I GGNCC 2 cut(s) 135, 148
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 3 cut(s) 98, 404, 545
AvaII GGWCC 1 cut(s) 135
BaeGI GKGCMC 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 616
BbvI GCAGC 1 cut(s) 575
BccI CCATC 1 cut(s) 209
BceAI ACGGC 4 cut(s) 97, 142, 219, 382
BciT130I CCWGG 1 cut(s) 321
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 309
BglI GCCNNNNNGGC 1 cut(s) 240
Bme1390I CCNGG 2 cut(s) 240, 321
Bme18I GGWCC 1 cut(s) 135
BmgT120I GGNCC 2 cut(s) 135, 148
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 2 cut(s) 240, 321
BoxI GACNNNNGTC 1 cut(s) 461
BpiI GAAGAC 1 cut(s) 616
BpmI CTGGAG 1 cut(s) 342
BpuMI CCSGG 1 cut(s) 240
BsaHI GRCGYC 3 cut(s) 9, 207, 462
BsaI GGTCTC 1 cut(s) 309
BsaJI CCNNGG 4 cut(s) 21, 250, 256, 319
BsaWI WCCGGW 1 cut(s) 132
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 145, 473
Bse1I ACTGG 1 cut(s) 463
BseAI TCCGGA 1 cut(s) 132
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 4 cut(s) 21, 250, 256, 319
BseGI GGATG 1 cut(s) 581
BseLI CCNNNNNNNGG 3 cut(s) 80, 145, 473
BseMII CTCAG 1 cut(s) 455
BseNI ACTGG 1 cut(s) 463
BsePI GCGCGC 1 cut(s) 45
BseSI GKGCMC 1 cut(s) 29
BseX3I CGGCCG 1 cut(s) 253
BseXI GCAGC 1 cut(s) 575
Bsh1236I CGCG 5 cut(s) 14, 47, 252, 258, 489
Bsh1285I CGRYCG 2 cut(s) 256, 262
BshFI GGCC 2 cut(s) 150, 255
BsiEI CGRYCG 2 cut(s) 256, 262
BsiSI CCGG 2 cut(s) 133, 240
BslFI GGGAC 3 cut(s) 222, 446, 449
BslI CCNNNNNNNGG 3 cut(s) 80, 145, 473
BsmAI GTCTC 1 cut(s) 309
BsmFI GGGAC 3 cut(s) 222, 446, 449
BsnI GGCC 2 cut(s) 150, 255
Bso31I GGTCTC 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 29
Bsp13I TCCGGA 1 cut(s) 132
Bsp143I GATC 3 cut(s) 91, 114, 227
BspANI GGCC 2 cut(s) 150, 255
BspCNI CTCAG 1 cut(s) 454
BspEI TCCGGA 1 cut(s) 132
BspFNI CGCG 5 cut(s) 14, 47, 252, 258, 489
BspLI GGNNCC 1 cut(s) 137
BspPI GGATC 3 cut(s) 99, 109, 222
BspTNI GGTCTC 1 cut(s) 309
BsrI ACTGG 1 cut(s) 463
BssECI CCNNGG 4 cut(s) 21, 250, 256, 319
BssHII GCGCGC 1 cut(s) 45
BssMI GATC 3 cut(s) 91, 114, 227
BssNI GRCGYC 3 cut(s) 9, 207, 462
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 3 cut(s) 31, 157, 437
Bst6I CTCTTC 1 cut(s) 101
BstACI GRCGYC 3 cut(s) 9, 207, 462
BstC8I GCNNGC 2 cut(s) 47, 524
BstDEI CTNAG 1 cut(s) 441
BstDSI CCRYGG 3 cut(s) 21, 250, 256
BstENI CCTNNNNNAGG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 581
BstFNI CGCG 5 cut(s) 14, 47, 252, 258, 489
BstHHI GCGC 2 cut(s) 47, 49
BstKTI GATC 3 cut(s) 94, 117, 230
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 3 cut(s) 91, 114, 227
BstMCI CGRYCG 2 cut(s) 256, 262
BstMWI GCNNNNNNNGC 8 cut(s) 11, 240, 249, 291, 366, 396, 405, 486
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 686
BstPAI GACNNNNGTC 1 cut(s) 461
BstSCI CCNGG 2 cut(s) 238, 319
BstSLI GKGCMC 1 cut(s) 29
BstUI CGCG 5 cut(s) 14, 47, 252, 258, 489
BstV1I GCAGC 1 cut(s) 575
BstV2I GAAGAC 1 cut(s) 616
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
BstZI CGGCCG 1 cut(s) 253
BsuRI GGCC 2 cut(s) 150, 255
BtgI CCRYGG 3 cut(s) 21, 250, 256
BtsCI GGATG 1 cut(s) 581
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 2 cut(s) 47, 524
CciNI GCGGCCGC 1 cut(s) 253
CfoI GCGC 2 cut(s) 47, 49
Cfr13I GGNCC 2 cut(s) 135, 148
Cfr42I CCGCGG 2 cut(s) 253, 259
CseI GACGC 4 cut(s) 17, 198, 451, 495
Csp6I GTAC 1 cut(s) 438
CspCI CAANNNNNGTGG 2 cut(s) 391, 426
CviAII CATG 1 cut(s) 683
CviQI GTAC 1 cut(s) 438
DdeI CTNAG 1 cut(s) 441
DpnI GATC 3 cut(s) 93, 116, 229
DpnII GATC 3 cut(s) 91, 114, 227
DrdI GACNNNNNNGTC 1 cut(s) 492
DseDI GACNNNNNNGTC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 253
EagI CGGCCG 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
EciI GGCGGA 3 cut(s) 303, 363, 384
EclXI CGGCCG 1 cut(s) 253
Eco31I GGTCTC 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 135
Eco52I CGGCCG 1 cut(s) 253
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 319
FaeI CATG 1 cut(s) 686
FaiI YATR 9 cut(s) 51, 68, 273, 300, 330, 504, 543, 621, 684
FaqI GGGAC 3 cut(s) 222, 446, 449
FatI CATG 1 cut(s) 682
FauI CCCGC 1 cut(s) 239
FokI GGATG 1 cut(s) 588
GlaI GCGC 2 cut(s) 46, 48
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 2 cut(s) 150, 255
HapII CCGG 2 cut(s) 133, 240
HgaI GACGC 4 cut(s) 17, 198, 451, 495
HhaI GCGC 2 cut(s) 47, 49
Hin1I GRCGYC 3 cut(s) 9, 207, 462
Hin1II CATG 1 cut(s) 686
Hin6I GCGC 2 cut(s) 45, 47
HinP1I GCGC 2 cut(s) 45, 47
HpaII CCGG 2 cut(s) 133, 240
HphI GGTGA 3 cut(s) 98, 404, 545
Hpy166II GTNNAC 2 cut(s) 103, 485
Hpy188I TCNGA 3 cut(s) 124, 145, 444
Hpy188III TCNNGA 3 cut(s) 74, 133, 466
Hpy8I GTNNAC 2 cut(s) 103, 485
Hpy99I CGWCG 6 cut(s) 80, 83, 128, 161, 209, 212
HpyAV CCTTC 4 cut(s) 297, 345, 551, 660
HpyCH4III ACNGT 3 cut(s) 31, 157, 437
HpyCH4IV ACGT 1 cut(s) 207
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 240, 249, 291, 366, 396, 405, 486
HpyF3I CTNAG 1 cut(s) 441
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 3 cut(s) 9, 207, 462
Hsp92II CATG 1 cut(s) 686
HspAI GCGC 2 cut(s) 45, 47
Kpn2I TCCGGA 1 cut(s) 132
KspI CCGCGG 2 cut(s) 253, 259
Kzo9I GATC 3 cut(s) 91, 114, 227
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 8 cut(s) 119, 146, 164, 253, 306, 333, 444, 508
Lsp1109I GCAGC 1 cut(s) 575
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 1 cut(s) 454
MalI GATC 3 cut(s) 93, 116, 229
MboI GATC 3 cut(s) 91, 114, 227
MboII GAAGA 2 cut(s) 88, 621
MfeI CAATTG 1 cut(s) 650
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 1 cut(s) 29
MluCI AATT 4 cut(s) 470, 544, 595, 650
MmeI TCCRAC 3 cut(s) 147, 193, 586
MroI TCCGGA 1 cut(s) 132
MseI TTAA 2 cut(s) 638, 691
MspA1I CMGCKG 2 cut(s) 252, 258
MspI CCGG 2 cut(s) 133, 240
MspR9I CCNGG 2 cut(s) 240, 321
MunI CAATTG 1 cut(s) 650
MvaI CCWGG 1 cut(s) 321
MvnI CGCG 5 cut(s) 14, 47, 252, 258, 489
MwoI GCNNNNNNNGC 8 cut(s) 11, 240, 249, 291, 366, 396, 405, 486
NciI CCSGG 1 cut(s) 240
NdeII GATC 3 cut(s) 91, 114, 227
NlaIII CATG 1 cut(s) 686
NlaIV GGNNCC 1 cut(s) 137
NmuCI GTSAC 1 cut(s) 454
NotI GCGGCCGC 1 cut(s) 253
NspI RCATGY 1 cut(s) 686
PauI GCGCGC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PshAI GACNNNNGTC 1 cut(s) 461
PsiI TTATAA 1 cut(s) 543
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 2 cut(s) 135, 148
PsuI RGATCY 1 cut(s) 91
PteI GCGCGC 1 cut(s) 45
RsaI GTAC 1 cut(s) 439
RsaNI GTAC 1 cut(s) 438
SacII CCGCGG 2 cut(s) 253, 259
SaqAI TTAA 2 cut(s) 638, 691
Sau3AI GATC 3 cut(s) 91, 114, 227
Sau96I GGNCC 2 cut(s) 135, 148
ScrFI CCNGG 2 cut(s) 240, 321
SduI GDGCHC 1 cut(s) 29
SetI ASST 6 cut(s) 73, 113, 210, 308, 376, 631
Sfr303I CCGCGG 2 cut(s) 253, 259
SgrBI CCGCGG 2 cut(s) 253, 259
SinI GGWCC 1 cut(s) 135
Sse9I AATT 4 cut(s) 470, 544, 595, 650
StyD4I CCNGG 2 cut(s) 238, 319
TaaI ACNGT 3 cut(s) 31, 157, 437
TaiI ACGT 1 cut(s) 210
TaqI TCGA 4 cut(s) 113, 159, 204, 226
TasI AATT 4 cut(s) 470, 544, 595, 650
TatI WGTACW 1 cut(s) 437
Tru1I TTAA 2 cut(s) 638, 691
Tru9I TTAA 2 cut(s) 638, 691
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 1 cut(s) 588
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 1 cut(s) 55
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 135
XagI CCTNNNNNAGG 1 cut(s) 143
XapI RAATTY 2 cut(s) 470, 595
XceI RCATGY 1 cut(s) 686
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.