pycom15g33090

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002479
Physical Location & Seq
Reverse (-)
32427942 .. 32430234
2293 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
ATGGAGTGCGTGGTCCAAGGAATTATAGAAACACAGCATGTTGAGGCCCTTGAGATTCTTCTTCAGGGTCTTTGTGGTGTTAGCAAGAAAAATTTGAGGGTCCATGAGATATGCCTTAAAAGTGGCCCAAACCTTGGGCTTGTAGCATCAGAGGTTCGACTTATATGCGATATTGAACAAAGTGAACAAACTGAACCGACATGGACTATAAGGCATATAGGGGGTGCAATGAGAGGTGCAGGTGCTGAGCAACTTTCAGTGCTAGTAAATCGATCCGTGGTTGAAAGCAAAACAAGCAAGAATGTGCTTCGCTTTTTTTATGCACTTGGATACAAGTTGGACCATGAGCTGCTAAGGGTGGGATTCTCCTTCCATTTCCAGAGGGGCACGCAGATCAATGTGACCGTGTCATCAGTTAATAAGATGCTACAACTACATGACGTAGATGCGGCTGTCCCTGTAACTCCTGGGATACAGCTGGTTGAAGTAACAGCTCCTGCTACATCTGAAAACTATGCTGAAGTTGTTGCTGCAGTATCATCTTTTAGTGAATATCTTGCACCGCTCCTGCATTTGTCAAAACCGGGCACTTCAACTGGGGTTGTTCCTACTGCTGCTGCGGCTGCTGCATCTCTTTTATCAGATGGTGGGGGTACGACCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000414 GO:0000428 GO:0001101 GO:0002831 GO:0003002 GO:0003006 GO:0003674 GO:0003712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006353 GO:0006355 GO:0006366 GO:0006369 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010219 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0023051 GO:0030880 GO:0031047 GO:0031050 GO:0031056 GO:0031060 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031399 GO:0031554 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032991 GO:0033043 GO:0033044 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043244 GO:0043254 GO:0043331 GO:0043900 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045892 GO:0045934 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048441 GO:0048442 GO:0048443 GO:0048464 GO:0048465 GO:0048466 GO:0048467 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048608 GO:0048731 GO:0048827 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051252 GO:0051253 GO:0051716 GO:0055029 GO:0060255 GO:0061458 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090567 GO:0090575 GO:0097305 GO:0097659 GO:0099402 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1902275 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000028 GO:2000031 GO:2000112 GO:2000113 GO:2000142 GO:2000241 GO:2001023 GO:2001038 GO:2001141 GO:2001253
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

23.55

Weight (kDa)

5.79

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 664
AarI CACCTGC 1 cut(s) 230
Acc36I ACCTGC 1 cut(s) 230
AccB7I CCANNNNNTGG 1 cut(s) 134
AccBSI CCGCTC 1 cut(s) 565
AciI CCGC 3 cut(s) 449, 563, 620
AclWI GGATC 1 cut(s) 267
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 2 cut(s) 47, 540
AfaI GTAC 1 cut(s) 655
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 4 cut(s) 176, 284, 485, 594
AjnI CCWGG 1 cut(s) 466
AluBI AGCT 3 cut(s) 349, 478, 494
AluI AGCT 3 cut(s) 349, 478, 494
AlwI GGATC 1 cut(s) 267
AlwNI CAGNNNCTG 2 cut(s) 245, 497
AoxI GGCC 2 cut(s) 45, 124
ApeKI GCWGC 6 cut(s) 349, 530, 614, 617, 623, 626
ApoI RAATTY 1 cut(s) 91
AspS9I GGNCC 5 cut(s) 13, 46, 100, 125, 340
AsuC2I CCSGG 1 cut(s) 585
AvaII GGWCC 3 cut(s) 13, 100, 340
BaeGI GKGCMC 2 cut(s) 389, 590
BbvI GCAGC 6 cut(s) 336, 517, 601, 604, 610, 613
BccI CCATC 1 cut(s) 638
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BciT130I CCWGG 1 cut(s) 468
BciVI GTATCC 2 cut(s) 323, 465
BcnI CCSGG 1 cut(s) 585
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 531
BfuAI ACCTGC 1 cut(s) 230
BfuI GTATCC 2 cut(s) 323, 465
BisI GCNGC 8 cut(s) 350, 450, 531, 615, 618, 621, 624, 627
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 8 cut(s) 351, 451, 532, 616, 619, 622, 625, 628
Bme1390I CCNGG 2 cut(s) 468, 585
Bme18I GGWCC 3 cut(s) 13, 100, 340
BmgT120I GGNCC 5 cut(s) 13, 46, 100, 125, 340
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 2 cut(s) 468, 585
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 4 cut(s) 155, 414, 436, 638
BmuI ACTGGG 1 cut(s) 606
Bpu10I CCTNAGC 1 cut(s) 353
Bpu1102I GCTNAGC 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 585
Bsa29I ATCGAT 1 cut(s) 271
BsaJI CCNNGG 4 cut(s) 16, 133, 276, 467
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 601
Bse3DI GCAATG 1 cut(s) 234
BseBI CCWGG 1 cut(s) 468
BseCI ATCGAT 1 cut(s) 271
BseDI CCNNGG 4 cut(s) 16, 133, 276, 467
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 1 cut(s) 601
BseSI GKGCMC 2 cut(s) 389, 590
BseXI GCAGC 6 cut(s) 336, 517, 601, 604, 610, 613
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 2 cut(s) 47, 126
BshVI ATCGAT 1 cut(s) 271
BsiSI CCGG 1 cut(s) 584
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 2 cut(s) 47, 126
Bsp1286I GDGCHC 2 cut(s) 389, 590
Bsp143I GATC 2 cut(s) 272, 393
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 3 cut(s) 449, 563, 620
BspANI GGCC 2 cut(s) 47, 126
BspCNI CTCAG 1 cut(s) 238
BspDI ATCGAT 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 101
BspMAI CTGCAG 1 cut(s) 535
BspMI ACCTGC 1 cut(s) 230
BspPI GGATC 1 cut(s) 267
BsrBI CCGCTC 1 cut(s) 565
BsrDI GCAATG 1 cut(s) 234
BsrI ACTGG 1 cut(s) 601
BssECI CCNNGG 4 cut(s) 16, 133, 276, 467
BssMI GATC 2 cut(s) 272, 393
BssT1I CCWWGG 2 cut(s) 16, 133
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 1 cut(s) 406
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 2 cut(s) 246, 353
BstDSI CCRYGG 1 cut(s) 276
BstKTI GATC 2 cut(s) 275, 396
BstMBI GATC 2 cut(s) 272, 393
BstMWI GCNNNNNNNGC 4 cut(s) 294, 620, 623, 626
BstNI CCWGG 1 cut(s) 468
BstNSI RCATGY 1 cut(s) 41
BstSCI CCNGG 2 cut(s) 466, 583
BstSFI CTRYAG 1 cut(s) 531
BstSLI GKGCMC 2 cut(s) 389, 590
BstV1I GCAGC 6 cut(s) 336, 517, 601, 604, 610, 613
Bsu15I ATCGAT 1 cut(s) 271
BsuI GTATCC 2 cut(s) 323, 465
BsuRI GGCC 2 cut(s) 47, 126
BsuTUI ATCGAT 1 cut(s) 271
BtgI CCRYGG 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 264
BveI ACCTGC 1 cut(s) 230
Cac8I GCNNGC 1 cut(s) 389
CaiI CAGNNNCTG 2 cut(s) 245, 497
Cfr13I GGNCC 5 cut(s) 13, 46, 100, 125, 340
ClaI ATCGAT 1 cut(s) 271
Csp6I GTAC 1 cut(s) 654
CviAII CATG 5 cut(s) 38, 104, 201, 344, 437
CviJI RGCY 8 cut(s) 47, 126, 139, 349, 452, 478, 494, 623
CviKI_1 RGCY 8 cut(s) 47, 126, 139, 349, 452, 478, 494, 623
CviQI GTAC 1 cut(s) 654
DdeI CTNAG 2 cut(s) 246, 353
DpnI GATC 2 cut(s) 274, 395
DpnII GATC 2 cut(s) 272, 393
Eco130I CCWWGG 2 cut(s) 16, 133
Eco47I GGWCC 3 cut(s) 13, 100, 340
Eco57I CTGAAG 2 cut(s) 47, 540
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 466
EcoT14I CCWWGG 2 cut(s) 16, 133
ErhI CCWWGG 2 cut(s) 16, 133
FaeI CATG 5 cut(s) 41, 107, 204, 347, 440
FaqI GGGAC 1 cut(s) 440
FatI CATG 5 cut(s) 37, 103, 200, 343, 436
Fnu4HI GCNGC 8 cut(s) 350, 450, 531, 615, 618, 621, 624, 627
Fsp4HI GCNGC 8 cut(s) 350, 450, 531, 615, 618, 621, 624, 627
FspBI CTAG 1 cut(s) 263
GluI GCNGC 8 cut(s) 350, 450, 531, 615, 618, 621, 624, 627
HaeIII GGCC 2 cut(s) 47, 126
HapII CCGG 1 cut(s) 584
Hin1II CATG 5 cut(s) 41, 107, 204, 347, 440
HinfI GANTC 2 cut(s) 55, 363
HpaII CCGG 1 cut(s) 584
Hpy166II GTNNAC 1 cut(s) 185
Hpy188I TCNGA 3 cut(s) 151, 508, 643
Hpy188III TCNNGA 1 cut(s) 379
Hpy8I GTNNAC 1 cut(s) 185
HpyAV CCTTC 1 cut(s) 379
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 7 cut(s) 227, 239, 323, 533, 560, 571, 629
HpyF10VI GCNNNNNNNGC 4 cut(s) 294, 620, 623, 626
HpyF3I CTNAG 2 cut(s) 246, 353
HpySE526I ACGT 1 cut(s) 441
Hsp92II CATG 5 cut(s) 41, 107, 204, 347, 440
Kzo9I GATC 2 cut(s) 272, 393
LmnI GCTCC 2 cut(s) 499, 570
Lsp1109I GCAGC 6 cut(s) 336, 517, 601, 604, 610, 613
LweI GCATC 4 cut(s) 155, 414, 436, 638
MaeI CTAG 1 cut(s) 263
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 3 cut(s) 400, 460, 487
MalI GATC 2 cut(s) 274, 395
MbiI CCGCTC 1 cut(s) 565
MboI GATC 2 cut(s) 272, 393
MboII GAAGA 2 cut(s) 50, 53
MhlI GDGCHC 2 cut(s) 389, 590
MluCI AATT 2 cut(s) 21, 91
MmeI TCCRAC 1 cut(s) 318
MnlI CCTC 5 cut(s) 37, 90, 145, 227, 375
MseI TTAA 2 cut(s) 117, 417
MspA1I CMGCKG 1 cut(s) 478
MspI CCGG 1 cut(s) 584
MspR9I CCNGG 2 cut(s) 468, 585
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 4 cut(s) 294, 620, 623, 626
NciI CCSGG 1 cut(s) 585
NdeII GATC 2 cut(s) 272, 393
NlaIII CATG 5 cut(s) 41, 107, 204, 347, 440
NlaIV GGNNCC 1 cut(s) 101
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 1 cut(s) 41
PaqCI CACCTGC 1 cut(s) 230
PfeI GAWTC 2 cut(s) 55, 363
PflFI GACNNNGTC 1 cut(s) 406
PflMI CCANNNNNTGG 1 cut(s) 134
PkrI GCNGC 8 cut(s) 351, 451, 532, 616, 619, 622, 625, 628
PsiI TTATAA 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 1 cut(s) 101
PspPI GGNCC 5 cut(s) 13, 46, 100, 125, 340
PstI CTGCAG 1 cut(s) 535
PstNI CAGNNNCTG 2 cut(s) 245, 497
PsyI GACNNNGTC 1 cut(s) 406
PvuII CAGCTG 1 cut(s) 478
RsaI GTAC 1 cut(s) 655
RsaNI GTAC 1 cut(s) 654
SaqAI TTAA 2 cut(s) 117, 417
SatI GCNGC 8 cut(s) 350, 450, 531, 615, 618, 621, 624, 627
Sau3AI GATC 2 cut(s) 272, 393
Sau96I GGNCC 5 cut(s) 13, 46, 100, 125, 340
ScrFI CCNGG 2 cut(s) 468, 585
SduI GDGCHC 2 cut(s) 389, 590
SetI ASST 9 cut(s) 135, 156, 238, 244, 351, 444, 480, 496, 662
SfaNI GCATC 4 cut(s) 155, 414, 436, 638
SfcI CTRYAG 1 cut(s) 531
SinI GGWCC 3 cut(s) 13, 100, 340
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 2 cut(s) 21, 91
SsiI CCGC 3 cut(s) 449, 563, 620
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 2 cut(s) 466, 583
StyI CCWWGG 2 cut(s) 16, 133
TaaI ACNGT 1 cut(s) 406
TaiI ACGT 1 cut(s) 444
TaqI TCGA 2 cut(s) 157, 271
TasI AATT 2 cut(s) 21, 91
TauI GCSGC 2 cut(s) 452, 623
TfiI GAWTC 2 cut(s) 55, 363
Tru1I TTAA 2 cut(s) 117, 417
Tru9I TTAA 2 cut(s) 117, 417
TscAI CASTG 1 cut(s) 264
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 6 cut(s) 349, 530, 614, 617, 623, 626
Tsp45I GTSAC 1 cut(s) 400
TspGWI ACGGA 1 cut(s) 265
TspRI CASTG 1 cut(s) 264
Tth111I GACNNNGTC 1 cut(s) 406
Van91I CCANNNNNTGG 1 cut(s) 134
VpaK11BI GGWCC 3 cut(s) 13, 100, 340
XapI RAATTY 1 cut(s) 91
XceI RCATGY 1 cut(s) 41
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.