pycom15g38330

Las1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001924
Physical Location & Seq
Reverse (-)
38136000 .. 38143671
7672 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1857 bp
ATGTTGTGTAGAGGTAAAGCGAGTGGAAGAGTTGTAATGGAAGAACTTTTGGGGTACACGGAAGAATCAAGAGATAATGAGGAGTTGGGTATATCAAATTGGGACGAGTGGCTCTTTGTCGAAAAATCTCTTTTCTCTAATTCACCTGATTTTGTGGCTTCCGCTTTGAGAAGAATATCAACATGGAGAAGCAGAGGCTGTCTTCCTATTACGATTGAAGTTACAGCATCAATTATTGAAATTCAACGAAAAGATCCTCACTTTAGAAAGGACCTGTCAAATGATGCTTTAGATAATTCCAGAACAGATCAATCACTTGATGCTTCACTCTCAGAGGAAATGCTGGCCATGTTATATTGTATGGCAATCATGAGGCTTGTGAATGGTGTGGTTGAGAAGACACGGAAAAAAACAGAGGTGTCAATTGCTGTGGCTGCTGATGCAATTGGCATTCCACGTACACTGATTGATGTTCGTCATGAGGGATCTCACCGTGAACTTCCTGCACTTGACGTTGTTCGTACTGCTTCAGTTAAGGCCCTTGATTGGTTGAAGTGTTATTACTGGGAGCCTCAGAAGAAGGACATTCCATTTCATGGTAATGAAACTGATAATATCAGAATTGAAATAAGGTCTAAATTGCATGAATTGGCCTTCTCCTTCAGAGTTAAGACGACTCCTCAATCCCATTCTTCAAATGTCAAGAAAAAGCGCGGTTTAAAAAAAGACCGTAAAAGGATATTGAAAAGTCTTATTGGGCTGTATTCTTCCTTCTCCTCAGAAGTTGTTTCTGTACTGTTGGAGTTCTTGTTGGAGGCTATAAATTCCTCAGACTCCTCGGGGCTTCCAGTAAACACCCAAAGTGGCCCAAGACTACTTATTTCGTTGAATGAGTGGAAGCTTGTGATAACAAAATTTTCAAACAAGGAACCAGAGTTGCTTCTGGCTCTTCTGAATGCAGTTCTTGATATGATTGAGACTCGGGAAACAGTGATATATGAGACGGGAGGGATTTCCACATCATCAGACCGCAGGGCTGAGGTACTCCAAGTTGAACACCTTTCTTCTCTCTTTGCGTGGCTCGTTCAAAATTTTGAAGGATTAAAGCCTCGCCATGAAATGGACTCTGCAGCCGAAATCAAAGTTTCTTCAGCAGGCAAAACTCTCTCAAAAGCAATTCTGATGGAACTCCTACGTAAATGTCTTATTGTATCAGCTTCTGCTAATAAAAAGTTCATGGATTCTGCCGCCCATCTTGCACAGCTGACTGGGGACAGCTTCCTAGTGGGAAAACTGCACAAGCTCTCTTCGTTTGTTTCATCAAATTCAGATGGTACTGAAGAAGATACTGTTCAAATAAATTCAAATAATCAATTTAAGAAGCAAGAAGAATCTATAAGTCAAGCAGCAGAGAAGCTAGAGCTGATTAAACGTCGCATAGTGAGGAGCAAAGTAGTGAAGAGGATCGATGCTGATGTCGGAGACACTAACAGATGGGTTCTGGCAAAATCATGGAACCCGTGTCCAATTGGAATGTTGCCTCGAGCTGTGGGGTCTTCTGGCCGCCTTCCTGTTCTGGATGTTAATGTCCTGGAAAAGGTTCCTGAGTTAGTAGGAAGAAAAGAGATGTGGGAGTTGAACCAATGCAGTGTCAAGAGACCTAGTTCCGAAATGCAGCTCCTAGATAACCCATGTGTTAAGAAAATGAGGGCGGCAGTGGATGGTTCTGTGTCGGAAGGTGAGGATCTTTCATCCTGGGAAGATGTCGACGGACATCTCATGATAGGCGGGGTGTGGAAGAAAGTCGGGGAAGAAGAGCTACTCGCCGTCAAGTCTGCGGTGAGGATATTAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Las1

Protein Analysis

619

Amino Acids

69.16

Weight (kDa)

7.59

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Las1 PF04031 32 - 191 4.1e-40 Las1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 596, 1120
AccI GTMKAC 1 cut(s) 1767
AccII CGCG 1 cut(s) 714
AciI CCGC 8 cut(s) 162, 714, 1030, 1248, 1564, 1712, 1788, 1838
AclWI GGATC 4 cut(s) 248, 493, 1472, 1752
AcoI YGGCCR 2 cut(s) 345, 1561
AcsI RAATTY 6 cut(s) 240, 823, 914, 1090, 1324, 1360
AcuI CTGAAG 4 cut(s) 513, 646, 1134, 1359
AfaI GTAC 6 cut(s) 56, 460, 523, 795, 1044, 1336
AfiI CCNNNNNNNGG 4 cut(s) 546, 596, 1120, 1597
AjnI CCWGG 2 cut(s) 1590, 1754
Alw26I GTCTC 4 cut(s) 971, 995, 1476, 1651
AlwI GGATC 4 cut(s) 248, 493, 1472, 1752
AlwNI CAGNNNCTG 2 cut(s) 198, 1220
Ama87I CYCGRG 3 cut(s) 838, 981, 1542
AoxI GGCC 5 cut(s) 345, 537, 651, 865, 1561
ApeKI GCWGC 4 cut(s) 434, 1130, 1406, 1675
ApoI RAATTY 6 cut(s) 240, 823, 914, 1090, 1324, 1360
AseI ATTAAT 1 cut(s) 1850
Asp700I GAANNNNTTC 1 cut(s) 1599
AspLEI GCGC 1 cut(s) 714
AspS9I GGNCC 3 cut(s) 271, 538, 866
AsuHPI GGTGA 4 cut(s) 135, 482, 1751, 1852
AvaI CYCGRG 3 cut(s) 838, 981, 1542
AvaII GGWCC 1 cut(s) 271
BalI TGGCCA 1 cut(s) 347
BarI GAAGNNNNNNTAC 6 cut(s) 545, 577, 755, 787, 1803, 1835
BbsI GAAGAC 3 cut(s) 194, 404, 1548
BbvCI CCTCAGC 1 cut(s) 1038
BbvI GCAGC 4 cut(s) 421, 1142, 1418, 1687
BccI CCATC 5 cut(s) 1177, 1260, 1325, 1488, 1715
BceAI ACGGC 1 cut(s) 1811
BciT130I CCWGG 2 cut(s) 1592, 1756
BcoDI GTCTC 4 cut(s) 971, 995, 1476, 1651
BfaI CTAG 5 cut(s) 1283, 1418, 1662, 1682, 1855
BfmI CTRYAG 1 cut(s) 1128
BisI GCNGC 7 cut(s) 435, 1131, 1248, 1407, 1564, 1676, 1713
BlsI GCNGC 7 cut(s) 436, 1132, 1249, 1408, 1565, 1677, 1714
Bme1390I CCNGG 2 cut(s) 1592, 1756
Bme18I GGWCC 1 cut(s) 271
BmeT110I CYCGRG 3 cut(s) 838, 981, 1542
BmgT120I GGNCC 3 cut(s) 271, 538, 866
BmiI GGNNCC 4 cut(s) 570, 930, 1517, 1602
BmrFI CCNGG 2 cut(s) 1592, 1756
BmrI ACTGGG 2 cut(s) 574, 1278
BmsI GCATC 5 cut(s) 236, 274, 310, 430, 1459
BmuI ACTGGG 2 cut(s) 574, 1278
BpiI GAAGAC 3 cut(s) 194, 404, 1548
Bpu10I CCTNAGC 1 cut(s) 1038
Bsa29I ATCGAT 1 cut(s) 1467
BsaAI YACGTR 2 cut(s) 458, 1196
BsaBI GATNNNNATC 1 cut(s) 1850
BsaI GGTCTC 1 cut(s) 1651
BsaJI CCNNGG 2 cut(s) 837, 1755
BsaXI ACNNNNNCTCC 2 cut(s) 794, 824
Bsc4I CCNNNNNNNGG 4 cut(s) 546, 596, 1120, 1597
Bse1I ACTGG 3 cut(s) 569, 848, 1273
Bse8I GATNNNNATC 1 cut(s) 1850
BseBI CCWGG 2 cut(s) 1592, 1756
BseCI ATCGAT 1 cut(s) 1467
BseDI CCNNGG 2 cut(s) 837, 1755
BseGI GGATG 3 cut(s) 1585, 1726, 1751
BseJI GATNNNNATC 1 cut(s) 1850
BseLI CCNNNNNNNGG 4 cut(s) 546, 596, 1120, 1597
BseMII CTCAG 6 cut(s) 345, 587, 792, 843, 1029, 1596
BseNI ACTGG 3 cut(s) 569, 848, 1273
BseRI GAGGAG 5 cut(s) 95, 669, 766, 826, 1459
BseXI GCAGC 4 cut(s) 421, 1142, 1418, 1687
BsgI GTGCAG 2 cut(s) 489, 1280
Bsh1236I CGCG 1 cut(s) 714
BshFI GGCC 5 cut(s) 347, 539, 653, 867, 1563
BshVI ATCGAT 1 cut(s) 1467
BsiHKCI CYCGRG 3 cut(s) 838, 981, 1542
BslFI GGGAC 2 cut(s) 116, 1286
BslI CCNNNNNNNGG 4 cut(s) 546, 596, 1120, 1597
BsmAI GTCTC 4 cut(s) 971, 995, 1476, 1651
BsmBI CGTCTC 1 cut(s) 995
BsmFI GGGAC 2 cut(s) 116, 1286
BsmI GAATGC 2 cut(s) 450, 961
BsnI GGCC 5 cut(s) 347, 539, 653, 867, 1563
Bso31I GGTCTC 1 cut(s) 1651
BsoBI CYCGRG 3 cut(s) 838, 981, 1542
Bsp143I GATC 5 cut(s) 253, 307, 485, 1464, 1744
BspACI CCGC 8 cut(s) 162, 714, 1030, 1248, 1564, 1712, 1788, 1838
BspANI GGCC 5 cut(s) 347, 539, 653, 867, 1563
BspCNI CTCAG 6 cut(s) 344, 586, 791, 842, 1030, 1597
BspDI ATCGAT 1 cut(s) 1467
BspFNI CGCG 1 cut(s) 714
BspHI TCATGA 3 cut(s) 369, 478, 1779
BspLI GGNNCC 4 cut(s) 570, 930, 1517, 1602
BspMAI CTGCAG 1 cut(s) 1132
BspPI GGATC 4 cut(s) 248, 493, 1472, 1752
BspQI GCTCTTC 2 cut(s) 954, 1809
BspTNI GGTCTC 1 cut(s) 1651
BsrI ACTGG 3 cut(s) 569, 848, 1273
BssECI CCNNGG 2 cut(s) 837, 1755
BssMI GATC 5 cut(s) 253, 307, 485, 1464, 1744
Bst2UI CCWGG 2 cut(s) 1592, 1756
Bst4CI ACNGT 5 cut(s) 494, 731, 798, 991, 1351
Bst6I CTCTTC 5 cut(s) 22, 954, 1312, 1454, 1809
BstBAI YACGTR 2 cut(s) 458, 1196
BstC8I GCNNGC 2 cut(s) 345, 1156
BstDEI CTNAG 6 cut(s) 331, 573, 778, 829, 1038, 1605
BstENI CCTNNNNNAGG 1 cut(s) 1595
BstF5I GGATG 3 cut(s) 1585, 1726, 1751
BstFNI CGCG 1 cut(s) 714
BstHHI GCGC 1 cut(s) 714
BstKTI GATC 5 cut(s) 256, 310, 488, 1467, 1747
BstMAI GTCTC 4 cut(s) 971, 995, 1476, 1651
BstMBI GATC 5 cut(s) 253, 307, 485, 1464, 1744
BstMWI GCNNNNNNNGC 3 cut(s) 434, 440, 1256
BstNI CCWGG 2 cut(s) 1592, 1756
BstSCI CCNGG 2 cut(s) 1590, 1754
BstSFI CTRYAG 1 cut(s) 1128
BstSNI TACGTA 1 cut(s) 1196
BstUI CGCG 1 cut(s) 714
BstV1I GCAGC 4 cut(s) 421, 1142, 1418, 1687
BstV2I GAAGAC 3 cut(s) 194, 404, 1548
BstX2I RGATCY 3 cut(s) 253, 485, 1744
BstYI RGATCY 3 cut(s) 253, 485, 1744
Bsu15I ATCGAT 1 cut(s) 1467
BsuRI GGCC 5 cut(s) 347, 539, 653, 867, 1563
BsuTUI ATCGAT 1 cut(s) 1467
BtsCI GGATG 3 cut(s) 1585, 1726, 1751
BtsI GCAGTG 2 cut(s) 1654, 1722
BtsIMutI CAGTG 4 cut(s) 461, 996, 1654, 1722
Cac8I GCNNGC 2 cut(s) 345, 1156
CaiI CAGNNNCTG 2 cut(s) 198, 1220
CciI TCATGA 3 cut(s) 369, 478, 1779
CfoI GCGC 1 cut(s) 714
Cfr13I GGNCC 3 cut(s) 271, 538, 866
ClaI ATCGAT 1 cut(s) 1467
Csp6I GTAC 6 cut(s) 55, 459, 522, 794, 1043, 1335
CspCI CAANNNNNGTGG 2 cut(s) 411, 446
CviQI GTAC 6 cut(s) 55, 459, 522, 794, 1043, 1335
DdeI CTNAG 6 cut(s) 331, 573, 778, 829, 1038, 1605
DpnI GATC 5 cut(s) 255, 309, 487, 1466, 1746
DpnII GATC 5 cut(s) 253, 307, 485, 1464, 1744
DraI TTTAAA 1 cut(s) 720
EaeI YGGCCR 2 cut(s) 345, 1561
Eam1104I CTCTTC 5 cut(s) 22, 954, 1312, 1454, 1809
EarI CTCTTC 5 cut(s) 22, 954, 1312, 1454, 1809
Eco105I TACGTA 1 cut(s) 1196
Eco31I GGTCTC 1 cut(s) 1651
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 4 cut(s) 513, 646, 1134, 1359
Eco88I CYCGRG 3 cut(s) 838, 981, 1542
EcoNI CCTNNNNNAGG 1 cut(s) 1595
EcoO109I RGGNCCY 2 cut(s) 271, 538
EcoRII CCWGG 2 cut(s) 1590, 1754
Esp3I CGTCTC 1 cut(s) 995
FaqI GGGAC 2 cut(s) 116, 1286
FauI CCCGC 1 cut(s) 1781
FblI GTMKAC 1 cut(s) 1767
Fnu4HI GCNGC 7 cut(s) 435, 1131, 1248, 1407, 1564, 1676, 1713
FokI GGATG 3 cut(s) 1592, 1733, 1738
Fsp4HI GCNGC 7 cut(s) 435, 1131, 1248, 1407, 1564, 1676, 1713
FspBI CTAG 5 cut(s) 1283, 1418, 1662, 1682, 1855
GlaI GCGC 1 cut(s) 713
GluI GCNGC 7 cut(s) 435, 1131, 1248, 1407, 1564, 1676, 1713
HaeIII GGCC 5 cut(s) 347, 539, 653, 867, 1563
HhaI GCGC 1 cut(s) 714
Hin6I GCGC 1 cut(s) 712
HinP1I GCGC 1 cut(s) 712
HincII GTYRAC 1 cut(s) 1768
HindII GTYRAC 1 cut(s) 1768
HindIII AAGCTT 1 cut(s) 899
HinfI GANTC 7 cut(s) 65, 676, 833, 979, 1124, 1241, 1391
HphI GGTGA 4 cut(s) 135, 482, 1751, 1852
Hpy166II GTNNAC 5 cut(s) 57, 461, 497, 853, 1768
Hpy8I GTNNAC 5 cut(s) 57, 461, 497, 853, 1768
Hpy99I CGWCG 2 cut(s) 1437, 1772
HpyAV CCTTC 7 cut(s) 574, 664, 670, 781, 1091, 1577, 1730
HpyCH4III ACNGT 5 cut(s) 494, 731, 798, 991, 1351
HpyCH4IV ACGT 4 cut(s) 457, 513, 1195, 1432
HpyCH4V TGCA 9 cut(s) 443, 506, 643, 959, 1130, 1259, 1297, 1647, 1675
HpyF10VI GCNNNNNNNGC 3 cut(s) 434, 440, 1256
HpyF3I CTNAG 6 cut(s) 331, 573, 778, 829, 1038, 1605
HpySE526I ACGT 4 cut(s) 457, 513, 1195, 1432
HspAI GCGC 1 cut(s) 712
Kzo9I GATC 5 cut(s) 253, 307, 485, 1464, 1744
LguI GCTCTTC 2 cut(s) 954, 1809
LmnI GCTCC 3 cut(s) 568, 1446, 1683
Lsp1109I GCAGC 4 cut(s) 421, 1142, 1418, 1687
LweI GCATC 5 cut(s) 236, 274, 310, 430, 1459
MaeI CTAG 5 cut(s) 1283, 1418, 1662, 1682, 1855
MaeII ACGT 4 cut(s) 457, 513, 1195, 1432
MaeIII GTNAC 1 cut(s) 220
MalI GATC 5 cut(s) 255, 309, 487, 1466, 1746
MboI GATC 5 cut(s) 253, 307, 485, 1464, 1744
MfeI CAATTG 3 cut(s) 423, 444, 1527
MflI RGATCY 3 cut(s) 253, 485, 1744
MlsI TGGCCA 1 cut(s) 347
MluNI TGGCCA 1 cut(s) 347
MlyI GAGTC 4 cut(s) 670, 827, 973, 1118
MmeI TCCRAC 4 cut(s) 780, 792, 1459, 1713
Mox20I TGGCCA 1 cut(s) 347
MroXI GAANNNNTTC 1 cut(s) 1599
MscI TGGCCA 1 cut(s) 347
MseI TTAA 9 cut(s) 534, 669, 719, 1103, 1377, 1428, 1584, 1698, 1850
MslI CAYNNNNRTG 1 cut(s) 600
Msp20I TGGCCA 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 1264
MspR9I CCNGG 2 cut(s) 1592, 1756
MunI CAATTG 3 cut(s) 423, 444, 1527
Mva1269I GAATGC 2 cut(s) 450, 961
MvaI CCWGG 2 cut(s) 1592, 1756
MvnI CGCG 1 cut(s) 714
MwoI GCNNNNNNNGC 3 cut(s) 434, 440, 1256
NdeII GATC 5 cut(s) 253, 307, 485, 1464, 1744
NlaIV GGNNCC 4 cut(s) 570, 930, 1517, 1602
PaeR7I CTCGAG 1 cut(s) 1542
PagI TCATGA 3 cut(s) 369, 478, 1779
PciSI GCTCTTC 2 cut(s) 954, 1809
PctI GAATGC 2 cut(s) 450, 961
PdmI GAANNNNTTC 1 cut(s) 1599
PfeI GAWTC 3 cut(s) 65, 1241, 1391
PflMI CCANNNNNTGG 2 cut(s) 596, 1120
PfoI TCCNGGA 1 cut(s) 1590
PkrI GCNGC 7 cut(s) 436, 1132, 1249, 1408, 1565, 1677, 1714
PleI GAGTC 4 cut(s) 670, 827, 973, 1118
PpsI GAGTC 4 cut(s) 670, 827, 973, 1118
Ppu21I YACGTR 2 cut(s) 458, 1196
PpuMI RGGWCCY 1 cut(s) 271
PshBI ATTAAT 1 cut(s) 1850
Psp5II RGGWCCY 1 cut(s) 271
Psp6I CCWGG 2 cut(s) 1590, 1754
PspGI CCWGG 2 cut(s) 1590, 1754
PspN4I GGNNCC 4 cut(s) 570, 930, 1517, 1602
PspPI GGNCC 3 cut(s) 271, 538, 866
PspPPI RGGWCCY 1 cut(s) 271
PspXI VCTCGAGB 1 cut(s) 1542
PstI CTGCAG 1 cut(s) 1132
PstNI CAGNNNCTG 2 cut(s) 198, 1220
PsuI RGATCY 3 cut(s) 253, 485, 1744
PvuII CAGCTG 1 cut(s) 1264
RsaI GTAC 6 cut(s) 56, 460, 523, 795, 1044, 1336
RsaNI GTAC 6 cut(s) 55, 459, 522, 794, 1043, 1335
RseI CAYNNNNRTG 1 cut(s) 600
SalI GTCGAC 1 cut(s) 1766
SapI GCTCTTC 2 cut(s) 954, 1809
SaqAI TTAA 9 cut(s) 534, 669, 719, 1103, 1377, 1428, 1584, 1698, 1850
SatI GCNGC 7 cut(s) 435, 1131, 1248, 1407, 1564, 1676, 1713
Sau3AI GATC 5 cut(s) 253, 307, 485, 1464, 1744
Sau96I GGNCC 3 cut(s) 271, 538, 866
SchI GAGTC 4 cut(s) 670, 827, 973, 1118
ScrFI CCNGG 2 cut(s) 1592, 1756
SfaNI GCATC 5 cut(s) 236, 274, 310, 430, 1459
SfcI CTRYAG 1 cut(s) 1128
Sfr274I CTCGAG 1 cut(s) 1542
SinI GGWCC 1 cut(s) 271
SlaI CTCGAG 1 cut(s) 1542
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 1 cut(s) 1542
SmoI CTYRAG 1 cut(s) 1542
SnaBI TACGTA 1 cut(s) 1196
SsiI CCGC 8 cut(s) 162, 714, 1030, 1248, 1564, 1712, 1788, 1838
SspMI CTAG 5 cut(s) 1283, 1418, 1662, 1682, 1855
StyD4I CCNGG 2 cut(s) 1590, 1754
TaaI ACNGT 5 cut(s) 494, 731, 798, 991, 1351
TaiI ACGT 4 cut(s) 460, 516, 1198, 1435
TaqI TCGA 4 cut(s) 120, 1467, 1543, 1767
TatI WGTACW 1 cut(s) 793
TauI GCSGC 3 cut(s) 1250, 1566, 1715
TfiI GAWTC 3 cut(s) 65, 1241, 1391
Tru1I TTAA 9 cut(s) 534, 669, 719, 1103, 1377, 1428, 1584, 1698, 1850
Tru9I TTAA 9 cut(s) 534, 669, 719, 1103, 1377, 1428, 1584, 1698, 1850
TscAI CASTG 4 cut(s) 468, 996, 1654, 1722
TseI GCWGC 4 cut(s) 434, 1130, 1406, 1675
TspDTI ATGAA 7 cut(s) 584, 618, 660, 1131, 1225, 1308, 1740
TspGWI ACGGA 3 cut(s) 74, 418, 1785
TspRI CASTG 4 cut(s) 468, 996, 1654, 1722
Van91I CCANNNNNTGG 2 cut(s) 596, 1120
VpaK11BI GGWCC 1 cut(s) 271
VspI ATTAAT 1 cut(s) 1850
XagI CCTNNNNNAGG 1 cut(s) 1595
XapI RAATTY 6 cut(s) 240, 823, 914, 1090, 1324, 1360
XhoI CTCGAG 1 cut(s) 1542
XmiI GTMKAC 1 cut(s) 1767
XmnI GAANNNNTTC 1 cut(s) 1599
XspI CTAG 5 cut(s) 1283, 1418, 1662, 1682, 1855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.