pycom15g38900

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003945
Physical Location & Seq
Forward (+)
38619678 .. 38620097
420 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 420 bp
ATGCCACAAAAGGGTTGTCAAAGAAATGAGGTTTCCTATACTAATTTGATTCATGGGTTTTGTGAGGCTGATCGGATCGATGAGGCTCTCAAGTTGTTTTCTCAAATGGGGGAGGATAATTGTTTTCCGACTGTGCGTACATTTACAGTTCCTATTTGCGCCTTGTGTAAATTGGGAAGGAAAATAGAAGCTATGAATCTGTTTAAAGAGATGATGGAAATGGGTTGTGAACCAAATGTTCATACTTATACCGTGCTCATTGATAGCATGTGTAAGGAAAATAAGCTTGATAAGGCAAGAAACTTGCTAAACAAGATGTTGGAGAAAAGGTTGGTTCCTAATGTCGTCACGTACAACGCAATGATTGATGGGTACTGCAAGGAGGGAGCAGCTGAGCTGCACTTGGTATTTTGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

15.96

Weight (kDa)

6.29

Isoelectric Point (pI)

26.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 1 - 50 8.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 4 - 36 7.3e-12 PPR repeat
PPR_2 PF13041 9 - 57 1.1e-15 PPR repeat family
PPR PF01535 11 - 41 3.8e-08 PPR repeat
PPR_1 PF12854 40 - 71 6.9e-06 PPR repeat
PPR_2 PF13041 52 - 92 1.4e-13 PPR repeat family
PPR_3 PF13812 67 - 125 8.9e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 74 - 106 3.5e-13 PPR repeat
PPR PF01535 82 - 110 3.8e-07 PPR repeat
PPR_2 PF13041 84 - 127 5.5e-15 PPR repeat family
PPR_1 PF12854 110 - 129 6.2e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 83
AfaI GTAC 3 cut(s) 139, 353, 374
AfiI CCNNNNNNNGG 1 cut(s) 11
AluBI AGCT 4 cut(s) 191, 286, 392, 397
AluI AGCT 4 cut(s) 191, 286, 392, 397
Alw21I GWGCWC 1 cut(s) 258
AlwI GGATC 1 cut(s) 83
ApeKI GCWGC 2 cut(s) 389, 397
ArsI GACNNNNNNTTYG 1 cut(s) 33
AspLEI GCGC 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 384, 401
BccI CCATC 2 cut(s) 208, 362
BisI GCNGC 2 cut(s) 390, 398
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 2 cut(s) 391, 399
BmiI GGNNCC 1 cut(s) 336
Bpu1102I GCTNAGC 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 74
Bsa29I ATCGAT 1 cut(s) 78
BsaAI YACGTR 1 cut(s) 351
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 366
BseCI ATCGAT 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 11
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 384
BseXI GCAGC 2 cut(s) 384, 401
BsgI GTGCAG 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 258
BslI CCNNNNNNNGG 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 2 cut(s) 70, 75
Bsp1720I GCTNAGC 1 cut(s) 393
BspCNI CTCAG 1 cut(s) 385
BspDI ATCGAT 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 336
BspPI GGATC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 366
BssMI GATC 2 cut(s) 70, 75
Bst4CI ACNGT 3 cut(s) 133, 148, 253
BstBAI YACGTR 1 cut(s) 351
BstDEI CTNAG 1 cut(s) 393
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 2 cut(s) 73, 78
BstMBI GATC 2 cut(s) 70, 75
BstNSI RCATGY 1 cut(s) 271
BstV1I GCAGC 2 cut(s) 384, 401
Bsu15I ATCGAT 1 cut(s) 78
BsuTUI ATCGAT 1 cut(s) 78
CfoI GCGC 1 cut(s) 161
ClaI ATCGAT 1 cut(s) 78
Csp6I GTAC 3 cut(s) 138, 352, 373
CviAII CATG 2 cut(s) 53, 268
CviJI RGCY 7 cut(s) 68, 86, 191, 286, 392, 397, 415
CviKI_1 RGCY 7 cut(s) 68, 86, 191, 286, 392, 397, 415
CviQI GTAC 3 cut(s) 138, 352, 373
DdeI CTNAG 1 cut(s) 393
DpnI GATC 2 cut(s) 72, 77
DpnII GATC 2 cut(s) 70, 75
DraI TTTAAA 1 cut(s) 205
FaeI CATG 2 cut(s) 56, 271
FaiI YATR 6 cut(s) 39, 54, 194, 243, 249, 269
FatI CATG 2 cut(s) 52, 267
Fnu4HI GCNGC 2 cut(s) 390, 398
Fsp4HI GCNGC 2 cut(s) 390, 398
GlaI GCGC 1 cut(s) 160
GluI GCNGC 2 cut(s) 390, 398
HhaI GCGC 1 cut(s) 161
Hin1II CATG 2 cut(s) 56, 271
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 2 cut(s) 49, 196
Hpy166II GTNNAC 1 cut(s) 230
Hpy188I TCNGA 2 cut(s) 75, 129
Hpy8I GTNNAC 1 cut(s) 230
HpyAV CCTTC 1 cut(s) 171
HpyCH4III ACNGT 3 cut(s) 133, 148, 253
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 2 cut(s) 378, 400
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 1 cut(s) 350
Hsp92II CATG 2 cut(s) 56, 271
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 2 cut(s) 70, 75
LmnI GCTCC 1 cut(s) 386
Lsp1109I GCAGC 2 cut(s) 384, 401
MaeII ACGT 1 cut(s) 350
MaeIII GTNAC 1 cut(s) 346
MalI GATC 2 cut(s) 72, 77
MboI GATC 2 cut(s) 70, 75
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 3 cut(s) 43, 118, 170
MmeI TCCRAC 2 cut(s) 152, 300
MnlI CCTC 5 cut(s) 22, 58, 76, 106, 376
MseI TTAA 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 392
NdeII GATC 2 cut(s) 70, 75
NlaIII CATG 2 cut(s) 56, 271
NlaIV GGNNCC 1 cut(s) 336
NmuCI GTSAC 1 cut(s) 346
NspI RCATGY 1 cut(s) 271
PfeI GAWTC 2 cut(s) 49, 196
PkrI GCNGC 2 cut(s) 391, 399
Ppu21I YACGTR 1 cut(s) 351
PspN4I GGNNCC 1 cut(s) 336
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 3 cut(s) 139, 353, 374
RsaNI GTAC 3 cut(s) 138, 352, 373
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 2 cut(s) 390, 398
Sau3AI GATC 2 cut(s) 70, 75
SduI GDGCHC 1 cut(s) 258
SetI ASST 7 cut(s) 33, 193, 288, 332, 353, 394, 399
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 3 cut(s) 43, 118, 170
TaaI ACNGT 3 cut(s) 133, 148, 253
TaiI ACGT 1 cut(s) 353
TaqI TCGA 1 cut(s) 78
TasI AATT 3 cut(s) 43, 118, 170
TfiI GAWTC 2 cut(s) 49, 196
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 2 cut(s) 389, 397
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 3 cut(s) 41, 209, 230
XceI RCATGY 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.