RchiOBHm_Chr5g0064611

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003945
Physical Location & Seq
Reverse (-)
70494668 .. 70496075
1408 bp
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UTR
Exon/CDS
Intron
PRQ34059

Sequence Viewer

Length: 510 bp
ATGGAGAACAGAGGCATGTCTCCTTCTAAAGTGACGTATACAATTGTAATTGATGCCTTGGTTCGATCTGATGACATGGAAAGAGCATTTCATGTGTTCTCCTCCATGGAGAAGGCTGGTCTGGTGCTTGATGTCTACACCTATGGGGTCTTAATACATGGATTATGCATGAAAGGTAATATGATTGAAGCATCAAAGCTGTTACACTCAATGAGCGATTCACATTTGGAGCCAAGTGATGTCATATTTAATATAATGATTCATGGCTGCTGCAAAGAGGGTAGCTCTTACAGGGCACTGAGGTTGCTCAAAGACATGAGAAAGAAAGGAATGGTTCCGAATGTGGCTAGTTATAGTTCGACCATTGGAGTTCTTTGTAATGATGGGAAATGGGAAGAAGCTGAAATACTACTTAAAGAAATGACGGACTCAGATTCGAAACCATCAGTTTCTTTGTATAATATAATTTCTAGAGTGAAAAATGATACACCGGTGAAGCTATCAACCTAA

Protein Analysis

169

Amino Acids

18.81

Weight (kDa)

6.51

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 4 - 37 3.9e-06 PPR repeat
PPR_2 PF13041 8 - 56 1.4e-15 PPR repeat family
PPR PF01535 11 - 41 1.9e-06 PPR repeat
PPR_3 PF13812 36 - 91 2.8e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 40 - 71 3.4e-09 PPR repeat
PPR_2 PF13041 51 - 92 9.8e-10 PPR repeat family
PPR_long PF17177 61 - 150 1e-05 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 76 - 107 1e-07 PPR repeat
PPR_2 PF13041 78 - 127 3.3e-14 PPR repeat family
PPR PF01535 81 - 111 5.4e-08 PPR repeat
PPR_3 PF13812 83 - 125 5.9e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 109 - 142 3e-06 PPR repeat
PPR_2 PF13041 113 - 156 9.6e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 38, 135
AgeI ACCGGT 1 cut(s) 490
AgsI TTSAA 1 cut(s) 188
AluBI AGCT 4 cut(s) 199, 285, 401, 499
AluI AGCT 4 cut(s) 199, 285, 401, 499
Alw26I GTCTC 1 cut(s) 24
ApeKI GCWGC 2 cut(s) 267, 270
AsiGI ACCGGT 1 cut(s) 490
AsuHPI GGTGA 1 cut(s) 505
AsuII TTCGAA 1 cut(s) 437
BaeGI GKGCMC 1 cut(s) 298
BaeI ACNNNNGTAYC 2 cut(s) 477, 510
BarI GAAGNNNNNNTAC 2 cut(s) 390, 422
BbvI GCAGC 2 cut(s) 254, 257
BccI CCATC 2 cut(s) 377, 451
BcoDI GTCTC 1 cut(s) 24
BfaI CTAG 2 cut(s) 348, 471
BisI GCNGC 2 cut(s) 268, 271
BlsI GCNGC 2 cut(s) 269, 272
BmiI GGNNCC 2 cut(s) 231, 336
BmsI GCATC 2 cut(s) 43, 200
BplI GAGNNNNNCTC 2 cut(s) 269, 301
Bpu14I TTCGAA 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 57, 105
BsaWI WCCGGW 1 cut(s) 490
Bse118I RCCGGY 1 cut(s) 490
BseDI CCNNGG 2 cut(s) 57, 105
BseMII CTCAG 2 cut(s) 290, 444
BseRI GAGGAG 1 cut(s) 91
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 2 cut(s) 254, 257
BshTI ACCGGT 1 cut(s) 490
BsiSI CCGG 1 cut(s) 491
BsmAI GTCTC 1 cut(s) 24
Bsp119I TTCGAA 1 cut(s) 437
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 1 cut(s) 65
Bsp19I CCATGG 1 cut(s) 105
BspCNI CTCAG 2 cut(s) 291, 443
BspLI GGNNCC 2 cut(s) 231, 336
BspT104I TTCGAA 1 cut(s) 437
BsrFI RCCGGY 1 cut(s) 490
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 2 cut(s) 57, 105
BssMI GATC 1 cut(s) 65
BssNAI GTATAC 1 cut(s) 39
BssT1I CCWWGG 2 cut(s) 57, 105
Bst1107I GTATAC 1 cut(s) 39
BstBI TTCGAA 1 cut(s) 437
BstDEI CTNAG 2 cut(s) 299, 430
BstDSI CCRYGG 1 cut(s) 105
BstKTI GATC 1 cut(s) 68
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 1 cut(s) 65
BstNSI RCATGY 1 cut(s) 19
BstSLI GKGCMC 1 cut(s) 298
BstV1I GCAGC 2 cut(s) 254, 257
BstZ17I GTATAC 1 cut(s) 39
BtgI CCRYGG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 490
CspAI ACCGGT 1 cut(s) 490
CviAII CATG 8 cut(s) 16, 76, 92, 106, 158, 169, 263, 316
CviJI RGCY 8 cut(s) 116, 199, 232, 267, 285, 347, 401, 499
CviKI_1 RGCY 8 cut(s) 116, 199, 232, 267, 285, 347, 401, 499
DdeI CTNAG 2 cut(s) 299, 430
DpnI GATC 1 cut(s) 67
DpnII GATC 1 cut(s) 65
Eco130I CCWWGG 2 cut(s) 57, 105
EcoT14I CCWWGG 2 cut(s) 57, 105
EcoT22I ATGCAT 1 cut(s) 170
ErhI CCWWGG 2 cut(s) 57, 105
FaeI CATG 8 cut(s) 19, 79, 95, 109, 161, 172, 266, 319
FatI CATG 8 cut(s) 15, 75, 91, 105, 157, 168, 262, 315
FblI GTMKAC 2 cut(s) 38, 135
Fnu4HI GCNGC 2 cut(s) 268, 271
Fsp4HI GCNGC 2 cut(s) 268, 271
FspBI CTAG 2 cut(s) 348, 471
GluI GCNGC 2 cut(s) 268, 271
HapII CCGG 1 cut(s) 491
Hin1II CATG 8 cut(s) 19, 79, 95, 109, 161, 172, 266, 319
HinfI GANTC 4 cut(s) 218, 259, 428, 434
HpaII CCGG 1 cut(s) 491
HphI GGTGA 1 cut(s) 505
Hpy166II GTNNAC 2 cut(s) 39, 136
Hpy188I TCNGA 3 cut(s) 70, 339, 433
Hpy188III TCNNGA 1 cut(s) 471
Hpy8I GTNNAC 2 cut(s) 39, 136
HpyAV CCTTC 2 cut(s) 33, 106
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 2 cut(s) 168, 273
HpyF3I CTNAG 2 cut(s) 299, 430
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 8 cut(s) 19, 79, 95, 109, 161, 172, 266, 319
Kzo9I GATC 1 cut(s) 65
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 4 cut(s) 102, 107, 277, 504
Lsp1109I GCAGC 2 cut(s) 254, 257
LweI GCATC 2 cut(s) 43, 200
MaeI CTAG 2 cut(s) 348, 471
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 2 cut(s) 31, 201
MalI GATC 1 cut(s) 67
MboI GATC 1 cut(s) 65
MboII GAAGA 1 cut(s) 407
MfeI CAATTG 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 3 cut(s) 42, 48, 465
MlyI GAGTC 1 cut(s) 422
MnlI CCTC 4 cut(s) 5, 112, 271, 294
Mph1103I ATGCAT 1 cut(s) 170
MseI TTAA 3 cut(s) 152, 249, 414
MspI CCGG 1 cut(s) 491
MunI CAATTG 1 cut(s) 42
NcoI CCATGG 1 cut(s) 105
NdeII GATC 1 cut(s) 65
NlaIII CATG 8 cut(s) 19, 79, 95, 109, 161, 172, 266, 319
NlaIV GGNNCC 2 cut(s) 231, 336
NmuCI GTSAC 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 170
NspI RCATGY 1 cut(s) 19
NspV TTCGAA 1 cut(s) 437
PfeI GAWTC 3 cut(s) 218, 259, 434
PinAI ACCGGT 1 cut(s) 490
PkrI GCNGC 2 cut(s) 269, 272
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
PspN4I GGNNCC 2 cut(s) 231, 336
SaqAI TTAA 3 cut(s) 152, 249, 414
SatI GCNGC 2 cut(s) 268, 271
Sau3AI GATC 1 cut(s) 65
SchI GAGTC 1 cut(s) 422
SduI GDGCHC 1 cut(s) 298
SetI ASST 9 cut(s) 38, 143, 178, 201, 287, 305, 403, 501, 509
SfaNI GCATC 2 cut(s) 43, 200
SfuI TTCGAA 1 cut(s) 437
SgrAI CRCCGGYG 1 cut(s) 490
Sse9I AATT 3 cut(s) 42, 48, 465
SspMI CTAG 2 cut(s) 348, 471
StyI CCWWGG 2 cut(s) 57, 105
TaiI ACGT 1 cut(s) 38
TaqI TCGA 3 cut(s) 64, 359, 437
TasI AATT 3 cut(s) 42, 48, 465
TfiI GAWTC 3 cut(s) 218, 259, 434
Tru1I TTAA 3 cut(s) 152, 249, 414
Tru9I TTAA 3 cut(s) 152, 249, 414
TscAI CASTG 1 cut(s) 303
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 2 cut(s) 267, 270
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 3 cut(s) 80, 185, 251
TspGWI ACGGA 1 cut(s) 440
TspRI CASTG 1 cut(s) 303
XbaI TCTAGA 1 cut(s) 470
XceI RCATGY 1 cut(s) 19
XmiI GTMKAC 2 cut(s) 38, 135
XspI CTAG 2 cut(s) 348, 471
Zsp2I ATGCAT 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.