RchiOBHm_Chr4g0385181

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003945
Physical Location & Seq
Reverse (-)
646986 .. 647910
925 bp
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UTR
Exon/CDS
Intron
PRQ35869

Sequence Viewer

Length: 915 bp
ATGAAGGATTACGGGTTTTACCCAACGGTTGAGTCATGCAATGTGTATGTGAGCTCATTGCTTGATTTACATAGAGCTGATGTTGCTTTGGCATTCTACAGAGAAATGAGGCGTTGTCGAATTTCACCCAATGTTTATACTCTTAATATGGTTATGTATGCTTATTGTAGAGTGGGAAAATTAGAGAATGCTGTTGAAGTCCTTGAGAAGATGGAGAGCATGGGTTTCAGTCCTACCATTGTGTCATATAATACACTTATTGCAGGACATTGCGATAAGGGTCTTCTGAGTGCCGCTGTGAAGTTCAAAAACTTGATGGTGAAGAATGGATTGCACCCGAATGTGGTAACTTTTAACACACTCATTGATGGGTTTTTTAAGCAAGGAAAACTACCAGAAGCAAATAGAATTTTTAGTGAGATGAAAGCCCTGAATGTGGCTCCTAATACAGTAACCTATAATACCATGATAAATGGGTATGGTCAAGCAGGTATTAGTGAGATGGGCAGTAGGCTTTTTGAGGAGATGTCAAAGGACCGAGTCAACGCTGATATTCTGACTTATTATGGATTGATTTTGGGACTATGCAAGGAGGGTAAGACAAAGAAAGCTGCATATCTGGTAAAAGAGCTTGACAAAAAGAACTTTGTCCCAAATGCCTCAACCTTTTCTGCACTTGTTGGTGGGCAACGTGTGAGAAAGAACTCAGATCGTGCCTTTCTGTTGTACAAGAGCATGGTTAGGAGTGGCTATCATCCCGATGAACATACTTTTAAGATGTTGTTGTCTAGCTTCTGCAATAATAGGGATTTTGATGGAGCAGTTGAAGTCTTGAGAGAAATTTTGTTCCTGATTCAGGCATATTATCTGATCTATGTCTGGGACTTCAACGCTGTGGAAAAGAAAAATGGGTAG

Protein Analysis

304

Amino Acids

34.48

Weight (kDa)

9.02

Isoelectric Point (pI)

26.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 8 - 57 1e-07 PPR repeat family
PPR_long PF17177 26 - 88 4.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 34 - 87 1.7e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 41 - 72 1.1e-08 PPR repeat
PPR_2 PF13041 47 - 81 4.3e-06 PPR repeat family
PPR PF01535 47 - 76 6e-06 PPR repeat
PPR_1 PF12854 74 - 107 3.4e-08 PPR repeat
PPR_2 PF13041 78 - 127 9.3e-17 PPR repeat family
PPR_3 PF13812 106 - 162 1.3e-14 Pentatricopeptide repeat domain
PPR_1 PF12854 109 - 142 2.5e-12 PPR repeat
PPR_2 PF13041 113 - 160 2.4e-19 PPR repeat family
PPR PF01535 116 - 145 2.5e-07 PPR repeat
PPR_1 PF12854 145 - 176 3.2e-07 PPR repeat
PPR_2 PF13041 148 - 197 6.5e-13 PPR repeat family
PPR PF01535 151 - 180 4.3e-07 PPR repeat
PPR_3 PF13812 171 - 228 2.8e-06 Pentatricopeptide repeat domain
PPR_long PF17177 172 - 277 7.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 191 - 229 2.4e-06 PPR repeat family
PPR_2 PF13041 234 - 267 3.3e-06 PPR repeat family
PPR_1 PF12854 250 - 280 4.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 479
AciI CCGC 1 cut(s) 294
AcsI RAATTY 3 cut(s) 120, 408, 840
AfaI GTAC 1 cut(s) 728
AfiI CCNNNNNNNGG 3 cut(s) 343, 436, 856
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 4 cut(s) 197, 307, 827, 889
AluBI AGCT 5 cut(s) 54, 77, 611, 631, 792
AluI AGCT 5 cut(s) 54, 77, 611, 631, 792
Alw21I GWGCWC 1 cut(s) 56
ApeKI GCWGC 1 cut(s) 611
ApoI RAATTY 3 cut(s) 120, 408, 840
AspS9I GGNCC 1 cut(s) 535
AsuHPI GGTGA 2 cut(s) 117, 331
AvaII GGWCC 1 cut(s) 535
BanII GRGCYC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 598
BccI CCATC 5 cut(s) 205, 310, 362, 496, 809
BfaI CTAG 1 cut(s) 789
BfmI CTRYAG 1 cut(s) 97
BfuAI ACCTGC 1 cut(s) 479
BisI GCNGC 2 cut(s) 294, 612
BlsI GCNGC 2 cut(s) 295, 613
Bme18I GGWCC 1 cut(s) 535
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 1 cut(s) 441
BpiI GAAGAC 1 cut(s) 275
BpuEI CTTGAG 2 cut(s) 224, 853
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 436, 856
Bse3DI GCAATG 3 cut(s) 46, 56, 268
BseGI GGATG 1 cut(s) 754
BseLI CCNNNNNNNGG 3 cut(s) 343, 436, 856
BseMI GCAATG 3 cut(s) 46, 56, 268
BseMII CTCAG 2 cut(s) 278, 720
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 1 cut(s) 598
BsgI GTGCAG 1 cut(s) 657
BsiHKAI GWGCWC 1 cut(s) 56
BslFI GGGAC 3 cut(s) 594, 635, 896
BslI CCNNNNNNNGG 3 cut(s) 343, 436, 856
BsmFI GGGAC 3 cut(s) 594, 635, 896
BsmI GAATGC 2 cut(s) 92, 193
Bsp1286I GDGCHC 1 cut(s) 56
Bsp1407I TGTACA 1 cut(s) 726
Bsp143I GATC 2 cut(s) 709, 870
BspACI CCGC 1 cut(s) 294
BspCNI CTCAG 2 cut(s) 279, 719
BspLI GGNNCC 1 cut(s) 441
BspMI ACCTGC 1 cut(s) 479
BsrDI GCAATG 3 cut(s) 46, 56, 268
BsrGI TGTACA 1 cut(s) 726
BssMI GATC 2 cut(s) 709, 870
Bst4CI ACNGT 2 cut(s) 28, 451
BstAUI TGTACA 1 cut(s) 726
BstDEI CTNAG 2 cut(s) 287, 706
BstENI CCTNNNNNAGG 1 cut(s) 854
BstF5I GGATG 1 cut(s) 754
BstKTI GATC 2 cut(s) 712, 873
BstMBI GATC 2 cut(s) 709, 870
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstSFI CTRYAG 1 cut(s) 97
BstV1I GCAGC 1 cut(s) 598
BstV2I GAAGAC 1 cut(s) 275
BtsCI GGATG 1 cut(s) 754
BveI ACCTGC 1 cut(s) 479
Cfr13I GGNCC 1 cut(s) 535
Csp6I GTAC 1 cut(s) 727
CviAII CATG 4 cut(s) 36, 220, 466, 736
CviJI RGCY 9 cut(s) 54, 77, 428, 440, 514, 611, 631, 750, 792
CviKI_1 RGCY 9 cut(s) 54, 77, 428, 440, 514, 611, 631, 750, 792
CviQI GTAC 1 cut(s) 727
DdeI CTNAG 2 cut(s) 287, 706
DpnI GATC 2 cut(s) 711, 872
DpnII GATC 2 cut(s) 709, 870
Ecl136II GAGCTC 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 535
Eco53kI GAGCTC 1 cut(s) 54
EcoICRI GAGCTC 1 cut(s) 54
EcoNI CCTNNNNNAGG 1 cut(s) 854
EcoT38I GRGCYC 1 cut(s) 56
FaeI CATG 4 cut(s) 39, 223, 469, 739
FaqI GGGAC 3 cut(s) 594, 635, 896
FatI CATG 4 cut(s) 35, 219, 465, 735
Fnu4HI GCNGC 2 cut(s) 294, 612
FokI GGATG 1 cut(s) 741
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 2 cut(s) 294, 612
FspBI CTAG 1 cut(s) 789
GluI GCNGC 2 cut(s) 294, 612
Hin1II CATG 4 cut(s) 39, 223, 469, 739
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HinfI GANTC 3 cut(s) 32, 540, 853
HphI GGTGA 2 cut(s) 117, 331
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 4 cut(s) 288, 558, 709, 870
Hpy188III TCNNGA 3 cut(s) 758, 832, 850
Hpy8I GTNNAC 1 cut(s) 544
HpyCH4III ACNGT 2 cut(s) 28, 451
HpyCH4IV ACGT 1 cut(s) 691
HpyCH4V TGCA 7 cut(s) 39, 263, 334, 588, 614, 674, 798
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 2 cut(s) 287, 706
HpySE526I ACGT 1 cut(s) 691
Hsp92II CATG 4 cut(s) 39, 223, 469, 739
Kzo9I GATC 2 cut(s) 709, 870
LmnI GCTCC 2 cut(s) 445, 818
LpnPI CCDG 8 cut(s) 249, 408, 443, 474, 605, 842, 863, 865
Lsp1109I GCAGC 1 cut(s) 598
MaeI CTAG 1 cut(s) 789
MaeII ACGT 1 cut(s) 691
MaeIII GTNAC 2 cut(s) 346, 451
MalI GATC 2 cut(s) 711, 872
MboI GATC 2 cut(s) 709, 870
MboII GAAGA 3 cut(s) 220, 275, 334
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 4 cut(s) 120, 179, 408, 840
MlyI GAGTC 2 cut(s) 41, 549
MnlI CCTC 4 cut(s) 102, 514, 586, 670
MseI TTAA 4 cut(s) 144, 354, 378, 774
MslI CAYNNNNRTG 2 cut(s) 339, 759
MspA1I CMGCKG 1 cut(s) 296
Mva1269I GAATGC 2 cut(s) 92, 193
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeII GATC 2 cut(s) 709, 870
NlaIII CATG 4 cut(s) 39, 223, 469, 739
NlaIV GGNNCC 1 cut(s) 441
PctI GAATGC 2 cut(s) 92, 193
PfeI GAWTC 1 cut(s) 853
PflFI GACNNNGTC 1 cut(s) 539
PkrI GCNGC 2 cut(s) 295, 613
PleI GAGTC 2 cut(s) 40, 548
PpsI GAGTC 2 cut(s) 40, 548
Psp124BI GAGCTC 1 cut(s) 56
PspN4I GGNNCC 1 cut(s) 441
PspPI GGNCC 1 cut(s) 535
PsyI GACNNNGTC 1 cut(s) 539
RsaI GTAC 1 cut(s) 728
RsaNI GTAC 1 cut(s) 727
RseI CAYNNNNRTG 2 cut(s) 339, 759
SacI GAGCTC 1 cut(s) 56
SaqAI TTAA 4 cut(s) 144, 354, 378, 774
SatI GCNGC 2 cut(s) 294, 612
Sau3AI GATC 2 cut(s) 709, 870
Sau96I GGNCC 1 cut(s) 535
SchI GAGTC 2 cut(s) 41, 549
SduI GDGCHC 1 cut(s) 56
SetI ASST 9 cut(s) 56, 79, 458, 493, 613, 633, 668, 694, 794
SfcI CTRYAG 1 cut(s) 97
SinI GGWCC 1 cut(s) 535
SmiMI CAYNNNNRTG 2 cut(s) 339, 759
SmlI CTYRAG 2 cut(s) 203, 832
SmoI CTYRAG 2 cut(s) 203, 832
Sse9I AATT 4 cut(s) 120, 179, 408, 840
SsiI CCGC 1 cut(s) 294
SspMI CTAG 1 cut(s) 789
SstI GAGCTC 1 cut(s) 56
TaaI ACNGT 2 cut(s) 28, 451
TaiI ACGT 1 cut(s) 694
TaqI TCGA 1 cut(s) 118
TaqII GACCGA 1 cut(s) 552
TasI AATT 4 cut(s) 120, 179, 408, 840
TatI WGTACW 1 cut(s) 726
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 1 cut(s) 853
Tru1I TTAA 4 cut(s) 144, 354, 378, 774
Tru9I TTAA 4 cut(s) 144, 354, 378, 774
TseI GCWGC 1 cut(s) 611
TspDTI ATGAA 3 cut(s) 17, 437, 777
Tth111I GACNNNGTC 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 535
XagI CCTNNNNNAGG 1 cut(s) 854
XapI RAATTY 3 cut(s) 120, 408, 840
XspI CTAG 1 cut(s) 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.