RLG00000019614

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003945
Physical Location & Seq
Reverse (-)
54269135 .. 54272727
3593 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019614

Sequence Viewer

Length: 645 bp
ATGAATTCAAAGAATTTGAGTGAGACCAAATCGATCCGTGTTTATGTTTCGGTTTCGAACCCCTCTTTAACGAAAAATGAAATTCCCTTTTCCAGTGATTACTCCGACGATTATAAAGATTGGTCACCTGGGATATTAGGCCAGCTGGCGGACCAAGTGACCAAGGCAGCAGACGACCTAGAACCAAGCCTAGACGACCCAACCCACTGCGAGTTCGCCGCCGACTTGGAGAAAATATACAGAATATTACACAAATTCCACTGCCGGGTCCCCAAACTAGAGCTCGCTCTACATCAATTCGGCGTCGTTTTGCACTCCGATTTAACTGAGCGAGTGTTGTCTCGGTGCTCGCTCTATATTGTGGGGTATAACAATTTGCTTGGTGGGTATGCTCAGGCTGAGAAGATGGTTGATGCGTATGAGCTGTTGAAGGAGATGGGGAGGAAGGATTGTGAGCCCAATGCGGGTTCGTATACGACTATGATTCAAGCACTTTGCGGGCAGGAGAAGATGGAGGAGGCGATGAGGGTGTTTGTGGAGATGGAGAGGAATGGTTGTGAGATTATGTTGGCTCATGAGAAGAAGGAAGACTTGGAGGAGTGTATGATTGATGGGACAAATGAAGAAGATTGGTTGCATGCCTGA

Protein Analysis

215

Amino Acids

24.34

Weight (kDa)

4.75

Isoelectric Point (pI)

31.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 120 - 166 1.6e-14 PPR repeat family
PPR_3 PF13812 120 - 165 9.1e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 142 - 187 1.8e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 149 - 182 7.5e-09 PPR repeat
PPR_2 PF13041 157 - 187 1.8e-08 PPR repeat family
PPR PF01535 157 - 186 4.2e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 114
AccI GTMKAC 1 cut(s) 473
AciI CCGC 4 cut(s) 149, 219, 464, 498
AclWI GGATC 1 cut(s) 28
AcsI RAATTY 4 cut(s) 4, 13, 81, 254
AcyI GRCGYC 1 cut(s) 303
AfiI CCNNNNNNNGG 3 cut(s) 148, 265, 464
AgsI TTSAA 3 cut(s) 9, 430, 488
AjnI CCWGG 1 cut(s) 127
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AluBI AGCT 3 cut(s) 145, 283, 424
AluI AGCT 3 cut(s) 145, 283, 424
Alw21I GWGCWC 2 cut(s) 285, 350
Alw26I GTCTC 2 cut(s) 17, 345
AlwI GGATC 1 cut(s) 28
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 167
ApoI RAATTY 4 cut(s) 4, 13, 81, 254
AspS9I GGNCC 2 cut(s) 151, 268
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 117
AsuII TTCGAA 1 cut(s) 56
AvaII GGWCC 2 cut(s) 151, 268
BanII GRGCYC 2 cut(s) 285, 459
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 2 cut(s) 285, 350
BbvI GCAGC 1 cut(s) 179
BccI CCATC 5 cut(s) 400, 430, 505, 535, 605
BciT130I CCWGG 1 cut(s) 129
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 2 cut(s) 17, 345
BfaI CTAG 3 cut(s) 179, 191, 278
BisI GCNGC 2 cut(s) 168, 219
BlsI GCNGC 2 cut(s) 169, 220
Bme1390I CCNGG 2 cut(s) 129, 266
Bme18I GGWCC 2 cut(s) 151, 268
BmgT120I GGNCC 2 cut(s) 151, 268
BmiI GGNNCC 2 cut(s) 269, 270
BmrFI CCNGG 2 cut(s) 129, 266
BmsI GCATC 1 cut(s) 403
BpiI GAAGAC 1 cut(s) 594
Bpu10I CCTNAGC 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 56
BpuMI CCSGG 1 cut(s) 266
Bsa29I ATCGAT 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 2 cut(s) 128, 162
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 265, 464
Bse1I ACTGG 1 cut(s) 93
BseBI CCWGG 1 cut(s) 129
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 128, 162
BseLI CCNNNNNNNGG 3 cut(s) 148, 265, 464
BseMII CTCAG 3 cut(s) 318, 390, 407
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 2 cut(s) 530, 611
BseXI GCAGC 1 cut(s) 179
BshFI GGCC 1 cut(s) 141
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 285, 350
BsiSI CCGG 1 cut(s) 265
BslFI GGGAC 2 cut(s) 254, 628
BslI CCNNNNNNNGG 3 cut(s) 148, 265, 464
BsmAI GTCTC 2 cut(s) 17, 345
BsmFI GGGAC 2 cut(s) 254, 628
BsnI GGCC 1 cut(s) 141
Bso31I GGTCTC 1 cut(s) 17
Bsp119I TTCGAA 1 cut(s) 56
Bsp1286I GDGCHC 3 cut(s) 285, 350, 459
Bsp143I GATC 1 cut(s) 33
BspACI CCGC 4 cut(s) 149, 219, 464, 498
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 3 cut(s) 319, 391, 406
BspDI ATCGAT 1 cut(s) 32
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 2 cut(s) 269, 270
BspPI GGATC 1 cut(s) 28
BspT104I TTCGAA 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 17
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 2 cut(s) 128, 162
BssMI GATC 1 cut(s) 33
BssNAI GTATAC 1 cut(s) 474
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 162
Bst1107I GTATAC 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 129
BstACI GRCGYC 1 cut(s) 303
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 6 cut(s) 143, 147, 285, 350, 500, 639
BstDEI CTNAG 3 cut(s) 327, 393, 399
BstEII GGTNACC 1 cut(s) 123
BstKTI GATC 1 cut(s) 36
BstMAI GTCTC 2 cut(s) 17, 345
BstMBI GATC 1 cut(s) 33
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 641
BstPI GGTNACC 1 cut(s) 123
BstSCI CCNGG 2 cut(s) 127, 264
BstV1I GCAGC 1 cut(s) 179
BstV2I GAAGAC 1 cut(s) 594
BstZ17I GTATAC 1 cut(s) 474
Bsu15I ATCGAT 1 cut(s) 32
BsuRI GGCC 1 cut(s) 141
BsuTUI ATCGAT 1 cut(s) 32
BtgZI GCGATG 1 cut(s) 536
BtsI GCAGTG 2 cut(s) 205, 259
BtsIMutI CAGTG 3 cut(s) 100, 205, 259
Cac8I GCNNGC 6 cut(s) 143, 147, 285, 350, 500, 639
CciI TCATGA 1 cut(s) 574
Cfr13I GGNCC 2 cut(s) 151, 268
ClaI ATCGAT 1 cut(s) 32
CseI GACGC 1 cut(s) 292
CviAII CATG 2 cut(s) 575, 638
CviJI RGCY 8 cut(s) 141, 145, 189, 283, 398, 424, 457, 572
CviKI_1 RGCY 8 cut(s) 141, 145, 189, 283, 398, 424, 457, 572
DdeI CTNAG 3 cut(s) 327, 393, 399
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
EciI GGCGGA 1 cut(s) 164
Ecl136II GAGCTC 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 2 cut(s) 285, 459
Eco31I GGTCTC 1 cut(s) 17
Eco47I GGWCC 2 cut(s) 151, 268
Eco53kI GAGCTC 1 cut(s) 283
Eco91I GGTNACC 1 cut(s) 123
EcoICRI GAGCTC 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 268
EcoO65I GGTNACC 1 cut(s) 123
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 2 cut(s) 285, 459
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 2 cut(s) 578, 641
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FaqI GGGAC 2 cut(s) 254, 628
FatI CATG 2 cut(s) 574, 637
FauI CCCGC 2 cut(s) 457, 491
FblI GTMKAC 1 cut(s) 473
Fnu4HI GCNGC 2 cut(s) 168, 219
FriOI GRGCYC 2 cut(s) 285, 459
Fsp4HI GCNGC 2 cut(s) 168, 219
FspBI CTAG 3 cut(s) 179, 191, 278
GluI GCNGC 2 cut(s) 168, 219
HaeIII GGCC 1 cut(s) 141
HapII CCGG 1 cut(s) 265
HgaI GACGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 2 cut(s) 578, 641
HinfI GANTC 1 cut(s) 484
HpaII CCGG 1 cut(s) 265
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 2 cut(s) 106, 319
Hpy188III TCNNGA 1 cut(s) 575
Hpy8I GTNNAC 1 cut(s) 474
Hpy99I CGWCG 2 cut(s) 110, 308
HpyAV CCTTC 3 cut(s) 424, 439, 577
HpyCH4V TGCA 2 cut(s) 313, 637
HpyF3I CTNAG 3 cut(s) 327, 393, 399
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 2 cut(s) 578, 641
KflI GGGWCCC 1 cut(s) 268
Kzo9I GATC 1 cut(s) 33
LpnPI CCDG 8 cut(s) 106, 114, 131, 141, 155, 278, 380, 488
Lsp1109I GCAGC 1 cut(s) 179
LweI GCATC 1 cut(s) 403
MaeI CTAG 3 cut(s) 179, 191, 278
MaeIII GTNAC 2 cut(s) 123, 157
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 6 cut(s) 415, 520, 592, 599, 635, 638
MhlI GDGCHC 3 cut(s) 285, 350, 459
MluCI AATT 6 cut(s) 4, 13, 81, 254, 296, 373
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 7 cut(s) 73, 435, 508, 511, 519, 540, 589
MseI TTAA 2 cut(s) 68, 323
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 1 cut(s) 265
MspR9I CCNGG 2 cut(s) 129, 266
MvaI CCWGG 1 cut(s) 129
NciI CCSGG 1 cut(s) 266
NdeII GATC 1 cut(s) 33
NlaIII CATG 2 cut(s) 578, 641
NlaIV GGNNCC 2 cut(s) 269, 270
NmuCI GTSAC 2 cut(s) 123, 157
NspI RCATGY 1 cut(s) 641
NspV TTCGAA 1 cut(s) 56
PaeI GCATGC 1 cut(s) 641
PagI TCATGA 1 cut(s) 574
PfeI GAWTC 1 cut(s) 484
PkrI GCNGC 2 cut(s) 169, 220
PpuMI RGGWCCY 1 cut(s) 268
PsiI TTATAA 1 cut(s) 114
Psp124BI GAGCTC 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 127
PspEI GGTNACC 1 cut(s) 123
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 2 cut(s) 269, 270
PspPI GGNCC 2 cut(s) 151, 268
PspPPI RGGWCCY 1 cut(s) 268
PvuII CAGCTG 1 cut(s) 145
SacI GAGCTC 1 cut(s) 285
SaqAI TTAA 2 cut(s) 68, 323
SatI GCNGC 2 cut(s) 168, 219
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 2 cut(s) 151, 268
ScrFI CCNGG 2 cut(s) 129, 266
SduI GDGCHC 3 cut(s) 285, 350, 459
SetI ASST 5 cut(s) 130, 147, 180, 285, 426
SfaNI GCATC 1 cut(s) 403
SfuI TTCGAA 1 cut(s) 56
SinI GGWCC 2 cut(s) 151, 268
SphI GCATGC 1 cut(s) 641
Sse9I AATT 6 cut(s) 4, 13, 81, 254, 296, 373
SsiI CCGC 4 cut(s) 149, 219, 464, 498
SspI AATATT 1 cut(s) 246
SspMI CTAG 3 cut(s) 179, 191, 278
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 127, 264
StyI CCWWGG 1 cut(s) 162
TaqI TCGA 2 cut(s) 32, 56
TasI AATT 6 cut(s) 4, 13, 81, 254, 296, 373
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 1 cut(s) 484
Tru1I TTAA 2 cut(s) 68, 323
Tru9I TTAA 2 cut(s) 68, 323
TscAI CASTG 3 cut(s) 100, 212, 266
TseFI GTSAC 2 cut(s) 123, 157
TseI GCWGC 1 cut(s) 167
Tsp45I GTSAC 2 cut(s) 123, 157
TspDTI ATGAA 3 cut(s) 17, 93, 636
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 3 cut(s) 100, 212, 266
VpaK11BI GGWCC 2 cut(s) 151, 268
XapI RAATTY 4 cut(s) 4, 13, 81, 254
XceI RCATGY 1 cut(s) 641
XmiI GTMKAC 1 cut(s) 473
XspI CTAG 3 cut(s) 179, 191, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.