RLG00000008165

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003945
Physical Location & Seq
Forward (+)
26436422 .. 26439047
2626 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008165

Sequence Viewer

Length: 705 bp
ATGCCGTTATGTAGAGTGGTGGAAAGAATAAGGGGAAAAGGGGCTAGTTTAGCACTTTTATGGAAAGAAGGCCAGCTGGCGGACCAAGTGACTAAGGCAGCAGACGACCCAGAACCAAGCCTAGACGACCCGACCCACTGCGAGTTCGGCCCCGACTTGGAGAAAATATACATAATATTACGCAAATTCCACTCCTGGGTCCCCAAACTAGAGCTGGCTCTACATCAATCAGGCGTCGTTTTGCACTCCGGCTTAACCGAGCGAGTGCTGTCTTGGTGCTCACTCTATATTGTGGGGTATAACAATTTGCTTGGTGGGTATGCTCAGGCAGTGAAGATGGCTGATGCGTATGAGCTGTTGAAGGAGATGAGGAGGAAGGGTTGTGAGCCCAATGCAGGTTCGTATACGACTATGATTCAAGCGCTTTGCGGGCAGGAGAAGATGGAGGAGGCGATGAGGGTGTTTGTGGGGATGGAGATCAGTGGTTGTGAGGTTGATATTGTGACTTATACTACTTTGATTAGTGGATTTTGTAAGTGGGGGAAGATTGTGAGGAGTTATGAGATTTTGGAGAGTATGATAAGGAAAGGGTTTACGCCGAGTCAGATGACTTACCTGCAGATTATGTTGGCTCATGAGAAGAAGGAAGACTTGGAGGAGTGTATAATTGATGGGGCAAATGAGGAAGATTGGTTGCATGCCTGA

Protein Analysis

235

Amino Acids

26.43

Weight (kDa)

5.24

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 97 - 143 1.4e-15 PPR repeat family
PPR_3 PF13812 97 - 142 1.4e-07 Pentatricopeptide repeat domain
PPR PF01535 100 - 128 8.5e-06 PPR repeat
PPR_long PF17177 112 - 205 5.7e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 126 - 159 2.7e-08 PPR repeat
PPR_2 PF13041 134 - 179 2.9e-15 PPR repeat family
PPR_3 PF13812 154 - 209 5.7e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 162 - 193 5.8e-10 PPR repeat
PPR_2 PF13041 166 - 206 3e-09 PPR repeat family
PPR PF01535 168 - 198 7.2e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 386, 624
AccI GTMKAC 1 cut(s) 404
AciI CCGC 2 cut(s) 80, 429
AcsI RAATTY 1 cut(s) 185
AcyI GRCGYC 1 cut(s) 234
AfeI AGCGCT 1 cut(s) 423
AfiI CCNNNNNNNGG 4 cut(s) 79, 157, 196, 395
AgsI TTSAA 2 cut(s) 361, 419
AjnI CCWGG 1 cut(s) 194
AjuI GAANNNNNNNTTGG 2 cut(s) 635, 667
AluBI AGCT 3 cut(s) 76, 214, 355
AluI AGCT 3 cut(s) 76, 214, 355
Alw21I GWGCWC 1 cut(s) 281
Aor51HI AGCGCT 1 cut(s) 423
AoxI GGCC 2 cut(s) 70, 148
ApeKI GCWGC 1 cut(s) 98
ApoI RAATTY 1 cut(s) 185
AspLEI GCGC 1 cut(s) 424
AspS9I GGNCC 3 cut(s) 82, 149, 199
AvaII GGWCC 2 cut(s) 82, 199
BanII GRGCYC 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 281
BbvI GCAGC 1 cut(s) 110
BccI CCATC 4 cut(s) 331, 436, 466, 665
BciT130I CCWGG 1 cut(s) 196
BfaI CTAG 3 cut(s) 45, 122, 209
BfmI CTRYAG 1 cut(s) 617
BfoI RGCGCY 1 cut(s) 425
BfuAI ACCTGC 2 cut(s) 386, 624
BisI GCNGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 100
Bme1390I CCNGG 1 cut(s) 196
Bme18I GGWCC 2 cut(s) 82, 199
BmgT120I GGNCC 3 cut(s) 82, 149, 199
BmiI GGNNCC 3 cut(s) 151, 200, 201
BmrFI CCNGG 1 cut(s) 196
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 1 cut(s) 654
Bpu10I CCTNAGC 1 cut(s) 324
BsaBI GATNNNNATC 1 cut(s) 476
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 195
BsaXI ACNNNNNCTCC 2 cut(s) 364, 394
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 157, 196, 395
Bse8I GATNNNNATC 1 cut(s) 476
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 1 cut(s) 477
BseJI GATNNNNATC 1 cut(s) 476
BseLI CCNNNNNNNGG 4 cut(s) 79, 157, 196, 395
BseMII CTCAG 1 cut(s) 338
BseRI GAGGAG 4 cut(s) 385, 461, 568, 671
BseXI GCAGC 1 cut(s) 110
BshFI GGCC 2 cut(s) 72, 150
BsiHKAI GWGCWC 1 cut(s) 281
BsiSI CCGG 1 cut(s) 249
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 4 cut(s) 79, 157, 196, 395
BsmFI GGGAC 1 cut(s) 185
BsnI GGCC 2 cut(s) 72, 150
Bsp1286I GDGCHC 2 cut(s) 281, 390
Bsp143I GATC 1 cut(s) 477
BspACI CCGC 2 cut(s) 80, 429
BspANI GGCC 2 cut(s) 72, 150
BspCNI CTCAG 1 cut(s) 337
BspHI TCATGA 1 cut(s) 634
BspLI GGNNCC 3 cut(s) 151, 200, 201
BspMAI CTGCAG 1 cut(s) 621
BspMI ACCTGC 2 cut(s) 386, 624
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 1 cut(s) 477
BssNAI GTATAC 1 cut(s) 405
BssNI GRCGYC 1 cut(s) 234
Bst1107I GTATAC 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 196
BstACI GRCGYC 1 cut(s) 234
BstC8I GCNNGC 5 cut(s) 74, 78, 216, 431, 699
BstDEI CTNAG 2 cut(s) 93, 324
BstF5I GGATG 1 cut(s) 477
BstH2I RGCGCY 1 cut(s) 425
BstHHI GCGC 1 cut(s) 424
BstKTI GATC 1 cut(s) 480
BstMBI GATC 1 cut(s) 477
BstMWI GCNNNNNNNGC 3 cut(s) 50, 147, 430
BstNI CCWGG 1 cut(s) 196
BstNSI RCATGY 1 cut(s) 701
BstSCI CCNGG 1 cut(s) 194
BstSFI CTRYAG 1 cut(s) 617
BstV1I GCAGC 1 cut(s) 110
BstV2I GAAGAC 1 cut(s) 654
BstZ17I GTATAC 1 cut(s) 405
BsuRI GGCC 2 cut(s) 72, 150
BtgZI GCGATG 1 cut(s) 467
BtsCI GGATG 1 cut(s) 477
BtsI GCAGTG 2 cut(s) 136, 336
BtsIMutI CAGTG 3 cut(s) 136, 336, 487
BveI ACCTGC 2 cut(s) 386, 624
Cac8I GCNNGC 5 cut(s) 74, 78, 216, 431, 699
CciI TCATGA 1 cut(s) 634
CfoI GCGC 1 cut(s) 424
Cfr13I GGNCC 3 cut(s) 82, 149, 199
CseI GACGC 1 cut(s) 223
CviAII CATG 2 cut(s) 635, 698
DdeI CTNAG 2 cut(s) 93, 324
DpnI GATC 1 cut(s) 479
DpnII GATC 1 cut(s) 477
EciI GGCGGA 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 390
Eco47I GGWCC 2 cut(s) 82, 199
Eco47III AGCGCT 1 cut(s) 423
EcoO109I RGGNCCY 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 194
EcoT38I GRGCYC 1 cut(s) 390
FaeI CATG 2 cut(s) 638, 701
FalI AAGNNNNNCTT 2 cut(s) 635, 667
FaqI GGGAC 1 cut(s) 185
FatI CATG 2 cut(s) 634, 697
FauI CCCGC 1 cut(s) 422
FblI GTMKAC 1 cut(s) 404
Fnu4HI GCNGC 1 cut(s) 99
FokI GGATG 1 cut(s) 484
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 1 cut(s) 99
FspBI CTAG 3 cut(s) 45, 122, 209
GlaI GCGC 1 cut(s) 423
GluI GCNGC 1 cut(s) 99
HaeII RGCGCY 1 cut(s) 425
HaeIII GGCC 2 cut(s) 72, 150
HapII CCGG 1 cut(s) 249
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 424
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 2 cut(s) 638, 701
Hin6I GCGC 1 cut(s) 422
HinP1I GCGC 1 cut(s) 422
HinfI GANTC 2 cut(s) 415, 601
HpaII CCGG 1 cut(s) 249
Hpy166II GTNNAC 2 cut(s) 405, 594
Hpy188I TCNGA 1 cut(s) 606
Hpy188III TCNNGA 1 cut(s) 635
Hpy8I GTNNAC 2 cut(s) 405, 594
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 4 cut(s) 62, 355, 370, 637
HpyCH4V TGCA 4 cut(s) 244, 395, 619, 697
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 147, 430
HpyF3I CTNAG 2 cut(s) 93, 324
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 2 cut(s) 638, 701
HspAI GCGC 1 cut(s) 422
KflI GGGWCCC 1 cut(s) 199
Kzo9I GATC 1 cut(s) 477
Lsp1109I GCAGC 1 cut(s) 110
LweI GCATC 1 cut(s) 334
MaeI CTAG 3 cut(s) 45, 122, 209
MaeIII GTNAC 2 cut(s) 88, 502
MalI GATC 1 cut(s) 479
MboI GATC 1 cut(s) 477
MboII GAAGA 6 cut(s) 346, 451, 556, 652, 659, 698
MhlI GDGCHC 2 cut(s) 281, 390
MluCI AATT 3 cut(s) 185, 304, 666
MlyI GAGTC 1 cut(s) 610
MnlI CCTC 9 cut(s) 363, 366, 439, 442, 450, 484, 546, 649, 676
MseI TTAA 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 58
MspA1I CMGCKG 1 cut(s) 76
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 1 cut(s) 196
MvaI CCWGG 1 cut(s) 196
MwoI GCNNNNNNNGC 3 cut(s) 50, 147, 430
NdeII GATC 1 cut(s) 477
NlaIII CATG 2 cut(s) 638, 701
NlaIV GGNNCC 3 cut(s) 151, 200, 201
NmeAIII GCCGAG 1 cut(s) 624
NmuCI GTSAC 2 cut(s) 88, 502
NspI RCATGY 1 cut(s) 701
PaeI GCATGC 1 cut(s) 701
PagI TCATGA 1 cut(s) 634
PfeI GAWTC 1 cut(s) 415
PkrI GCNGC 1 cut(s) 100
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
PpuMI RGGWCCY 1 cut(s) 199
Psp5II RGGWCCY 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 194
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 3 cut(s) 151, 200, 201
PspPI GGNCC 3 cut(s) 82, 149, 199
PspPPI RGGWCCY 1 cut(s) 199
PstI CTGCAG 1 cut(s) 621
PvuII CAGCTG 1 cut(s) 76
RseI CAYNNNNRTG 1 cut(s) 58
SaqAI TTAA 1 cut(s) 254
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 1 cut(s) 477
Sau96I GGNCC 3 cut(s) 82, 149, 199
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 1 cut(s) 196
SduI GDGCHC 2 cut(s) 281, 390
SetI ASST 6 cut(s) 78, 216, 357, 400, 495, 618
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 617
SinI GGWCC 2 cut(s) 82, 199
SmiMI CAYNNNNRTG 1 cut(s) 58
SphI GCATGC 1 cut(s) 701
Sse9I AATT 3 cut(s) 185, 304, 666
SsiI CCGC 2 cut(s) 80, 429
SspI AATATT 1 cut(s) 177
SspMI CTAG 3 cut(s) 45, 122, 209
StyD4I CCNGG 1 cut(s) 194
TasI AATT 3 cut(s) 185, 304, 666
TfiI GAWTC 1 cut(s) 415
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TscAI CASTG 3 cut(s) 143, 336, 487
TseFI GTSAC 2 cut(s) 88, 502
TseI GCWGC 1 cut(s) 98
Tsp45I GTSAC 2 cut(s) 88, 502
TspRI CASTG 3 cut(s) 143, 336, 487
VpaK11BI GGWCC 2 cut(s) 82, 199
XapI RAATTY 1 cut(s) 185
XceI RCATGY 1 cut(s) 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XmiI GTMKAC 1 cut(s) 404
XspI CTAG 3 cut(s) 45, 122, 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.