pycom16g04290

Coiled-coil domain-containing protein 130-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006850
Physical Location & Seq
Reverse (-)
2699467 .. 2702937
3471 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1113 bp
ATGTCCAACAGGAAAAAATTGAGAACCGACCAGGAAAGGGGGCATAAGAAAATTTCAGGAACCAAAACTTCAACGGAGCGATTGAATCGAGCGGAACCACTCCGGCGACCGAGTAGCAATTCAGGAAAGAGAAAATGGACAGATAGAAATACTTGGGCTTGTGATGAATCTTTTTCTACACTTGCAGCTGCTAGGGCAGACAACTTTTATTATCCCCCAGAATGGTCGCCAAAGAAGGGTTCCTTGAACAAGTTCAATGGTCAACATGCTCTGAGGGAGAGAGCAAGGAAATTGGACCAGGGGATTTTGATTATAAGGTTCGAGATGCCTTACCATATATGGTGCGGTGGTTGCAATTCTATGATTGCAAAGGGTGTTCGTTTTAATGCAGAAAAAAAGCAAGTTGGAAATTACTATTCTACAAAGATATGGAGCTTTACCATGAAGTCTCCATGCTGCAAACAAGAGATTGTTATGCAGATAGATCCAAAGAATTGCAAATATGTGATTATTAGTGGGGCCACACAGAAGAATGAGGAATTTGACGTTGAGGATGCGGAAACCTTTGAACTTCCTGCTGATGAAGAAAGAGGAAAGCTTGCGGATCCATTTTATCGACTTGAACATCAAGAAGAGGATTTGCAAAAGAAGAAAGAAGCCGAGCCAGTACTCGTTCGCCTACAGCGAATTTCTGATGACAGGCATTTAGATGACTATTCCCTCAACAAGGCCCTCAGAGCCAAACTTAGATCTCAAAAGAAAAGAGTTGCCGAAGAAGAGGCTACGTCAAGGAAGATGGGTCTCGGTATACGACTGCTTCCGGTGGCAGAGGAGGATGCTGCTGCTGCATCACGTGTGAAGTTCGTTTCCAAGTTTGAAAAAAATAGGAAGAATAAGCAAGCACTAATCAACGCTGCTTCAATTTTTCCTGGCTCATCTGGGAGTTCTATGTCGAATAAGAAGAGTTTGGAACTACAATCTAAGAGAAGAAGAATAAGTGCTGCTGCTGCGTCCAACTTACTTACAGGCGGGTTCAAGCCATCATCATGGTCTCAGAATACGGTTTCTTCAGGCAGGCTAAATGGAACGGCAGTCAAAGTGAAACGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

371

Amino Acids

42.06

Weight (kDa)

9.91

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 70 - 331 3.2e-61 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
AccBSI CCGCTC 1 cut(s) 92
AccI GTMKAC 1 cut(s) 808
AciI CCGC 5 cut(s) 92, 345, 557, 602, 1029
AclWI GGATC 3 cut(s) 479, 599, 612
AcsI RAATTY 3 cut(s) 51, 539, 687
AcuI CTGAAG 1 cut(s) 1053
AcvI CACGTG 1 cut(s) 854
AfaI GTAC 1 cut(s) 669
AfiI CCNNNNNNNGG 4 cut(s) 37, 222, 236, 727
AflIII ACRYGT 1 cut(s) 853
AgsI TTSAA 9 cut(s) 72, 85, 247, 256, 569, 623, 878, 921, 1036
AjnI CCWGG 3 cut(s) 30, 297, 928
AjuI GAANNNNNNNTTGG 2 cut(s) 223, 255
AluBI AGCT 3 cut(s) 188, 435, 598
AluI AGCT 3 cut(s) 188, 435, 598
Alw26I GTCTC 3 cut(s) 453, 806, 1056
AlwI GGATC 3 cut(s) 479, 599, 612
AoxI GGCC 2 cut(s) 519, 729
ApoI RAATTY 3 cut(s) 51, 539, 687
Asp700I GAANNNNTTC 2 cut(s) 251, 1106
AspS9I GGNCC 3 cut(s) 295, 519, 730
AvaII GGWCC 1 cut(s) 295
BamHI GGATCC 1 cut(s) 604
BbrPI CACGTG 1 cut(s) 854
BccI CCATC 2 cut(s) 790, 1048
BceAI ACGGC 1 cut(s) 1104
BciT130I CCWGG 3 cut(s) 32, 299, 930
BcoDI GTCTC 3 cut(s) 453, 806, 1056
BfaI CTAG 2 cut(s) 192, 1111
BfmI CTRYAG 1 cut(s) 680
BglII AGATCT 1 cut(s) 749
BmcAI AGTACT 1 cut(s) 669
Bme1390I CCNGG 3 cut(s) 32, 299, 930
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 3 cut(s) 295, 519, 730
BmiI GGNNCC 5 cut(s) 61, 96, 241, 520, 606
BmrFI CCNGG 3 cut(s) 32, 299, 930
BmsI GCATC 4 cut(s) 315, 544, 826, 857
BsaAI YACGTR 1 cut(s) 854
BsaI GGTCTC 2 cut(s) 806, 1056
BsaJI CCNNGG 1 cut(s) 298
BsaWI WCCGGW 1 cut(s) 820
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 222, 236, 727
Bse1I ACTGG 1 cut(s) 665
BseBI CCWGG 3 cut(s) 32, 299, 930
BseDI CCNNGG 1 cut(s) 298
BseGI GGATG 2 cut(s) 559, 841
BseLI CCNNNNNNNGG 4 cut(s) 37, 222, 236, 727
BseMII CTCAG 3 cut(s) 263, 748, 1067
BseNI ACTGG 1 cut(s) 665
BseRI GAGGAG 1 cut(s) 845
Bsh1285I CGRYCG 1 cut(s) 110
BshFI GGCC 2 cut(s) 521, 731
BsiEI CGRYCG 1 cut(s) 110
BsiSI CCGG 2 cut(s) 103, 821
BslI CCNNNNNNNGG 4 cut(s) 37, 222, 236, 727
BsmAI GTCTC 3 cut(s) 453, 806, 1056
BsnI GGCC 2 cut(s) 521, 731
Bso31I GGTCTC 2 cut(s) 806, 1056
Bsp143I GATC 3 cut(s) 484, 604, 749
BspACI CCGC 5 cut(s) 92, 345, 557, 602, 1029
BspANI GGCC 2 cut(s) 521, 731
BspCNI CTCAG 3 cut(s) 264, 747, 1066
BspLI GGNNCC 5 cut(s) 61, 96, 241, 520, 606
BspPI GGATC 3 cut(s) 479, 599, 612
BspTNI GGTCTC 2 cut(s) 806, 1056
BsrBI CCGCTC 1 cut(s) 92
BsrI ACTGG 1 cut(s) 665
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 3 cut(s) 484, 604, 749
BssNAI GTATAC 1 cut(s) 809
Bst1107I GTATAC 1 cut(s) 809
Bst2UI CCWGG 3 cut(s) 32, 299, 930
Bst4CI ACNGT 1 cut(s) 1063
Bst6I CTCTTC 3 cut(s) 627, 771, 956
BstBAI YACGTR 1 cut(s) 854
BstC8I GCNNGC 3 cut(s) 600, 900, 1076
BstDEI CTNAG 5 cut(s) 272, 734, 746, 981, 1053
BstENI CCTNNNNNAGG 1 cut(s) 725
BstF5I GGATG 2 cut(s) 559, 841
BstKTI GATC 3 cut(s) 487, 607, 752
BstMAI GTCTC 3 cut(s) 453, 806, 1056
BstMBI GATC 3 cut(s) 484, 604, 749
BstMCI CGRYCG 1 cut(s) 110
BstMWI GCNNNNNNNGC 5 cut(s) 194, 351, 737, 845, 1007
BstNI CCWGG 3 cut(s) 32, 299, 930
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 3 cut(s) 30, 297, 928
BstSFI CTRYAG 1 cut(s) 680
BstX2I RGATCY 3 cut(s) 484, 604, 749
BstXI CCANNNNNNTGG 1 cut(s) 1047
BstYI RGATCY 3 cut(s) 484, 604, 749
BstZ17I GTATAC 1 cut(s) 809
BsuRI GGCC 2 cut(s) 521, 731
BtsCI GGATG 2 cut(s) 559, 841
Cac8I GCNNGC 3 cut(s) 600, 900, 1076
Cfr13I GGNCC 3 cut(s) 295, 519, 730
CseI GACGC 1 cut(s) 999
Csp6I GTAC 1 cut(s) 668
CviAII CATG 4 cut(s) 266, 442, 453, 1047
CviQI GTAC 1 cut(s) 668
DdeI CTNAG 5 cut(s) 272, 734, 746, 981, 1053
DpnI GATC 3 cut(s) 486, 606, 751
DpnII GATC 3 cut(s) 484, 604, 749
Eam1104I CTCTTC 3 cut(s) 627, 771, 956
EarI CTCTTC 3 cut(s) 627, 771, 956
Eco31I GGTCTC 2 cut(s) 806, 1056
Eco47I GGWCC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 1053
Eco72I CACGTG 1 cut(s) 854
EcoNI CCTNNNNNAGG 1 cut(s) 725
EcoO109I RGGNCCY 1 cut(s) 730
EcoRII CCWGG 3 cut(s) 30, 297, 928
FaeI CATG 4 cut(s) 269, 445, 456, 1050
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FatI CATG 4 cut(s) 265, 441, 452, 1046
FauI CCCGC 1 cut(s) 1022
FblI GTMKAC 1 cut(s) 808
FokI GGATG 2 cut(s) 566, 848
FspBI CTAG 2 cut(s) 192, 1111
HaeIII GGCC 2 cut(s) 521, 731
HapII CCGG 2 cut(s) 103, 821
HgaI GACGC 1 cut(s) 999
Hin1II CATG 4 cut(s) 269, 445, 456, 1050
HincII GTYRAC 1 cut(s) 263
HindII GTYRAC 1 cut(s) 263
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 2 cut(s) 85, 167
HpaII CCGG 2 cut(s) 103, 821
Hpy166II GTNNAC 2 cut(s) 263, 809
Hpy188I TCNGA 4 cut(s) 273, 694, 737, 1056
Hpy188III TCNNGA 4 cut(s) 57, 123, 322, 629
Hpy8I GTNNAC 2 cut(s) 263, 809
HpyAV CCTTC 1 cut(s) 229
HpyCH4III ACNGT 1 cut(s) 1063
HpyCH4IV ACGT 3 cut(s) 546, 785, 853
HpyCH4V TGCA 9 cut(s) 185, 354, 368, 389, 459, 478, 498, 643, 848
HpyF10VI GCNNNNNNNGC 5 cut(s) 194, 351, 737, 845, 1007
HpyF3I CTNAG 5 cut(s) 272, 734, 746, 981, 1053
HpySE526I ACGT 3 cut(s) 546, 785, 853
Hsp92II CATG 4 cut(s) 269, 445, 456, 1050
Kzo9I GATC 3 cut(s) 484, 604, 749
LmnI GCTCC 2 cut(s) 76, 432
LweI GCATC 4 cut(s) 315, 544, 826, 857
MaeI CTAG 2 cut(s) 192, 1111
MaeII ACGT 3 cut(s) 546, 785, 853
MalI GATC 3 cut(s) 486, 606, 751
MbiI CCGCTC 1 cut(s) 92
MboI GATC 3 cut(s) 484, 604, 749
MflI RGATCY 3 cut(s) 484, 604, 749
MmeI TCCRAC 3 cut(s) 30, 385, 1038
MroXI GAANNNNTTC 2 cut(s) 251, 1106
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 2 cut(s) 708, 1045
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 2 cut(s) 103, 821
MspR9I CCNGG 3 cut(s) 32, 299, 930
MvaI CCWGG 3 cut(s) 32, 299, 930
MwoI GCNNNNNNNGC 5 cut(s) 194, 351, 737, 845, 1007
NdeII GATC 3 cut(s) 484, 604, 749
NlaIII CATG 4 cut(s) 269, 445, 456, 1050
NlaIV GGNNCC 5 cut(s) 61, 96, 241, 520, 606
NmeAIII GCCGAG 1 cut(s) 685
NspI RCATGY 1 cut(s) 269
PcsI WCGNNNNNNNCGW 1 cut(s) 682
PdmI GAANNNNTTC 2 cut(s) 251, 1106
PfeI GAWTC 2 cut(s) 85, 167
PmaCI CACGTG 1 cut(s) 854
PmlI CACGTG 1 cut(s) 854
Ppu21I YACGTR 1 cut(s) 854
PsiI TTATAA 1 cut(s) 314
Psp6I CCWGG 3 cut(s) 30, 297, 928
PspCI CACGTG 1 cut(s) 854
PspGI CCWGG 3 cut(s) 30, 297, 928
PspN4I GGNNCC 5 cut(s) 61, 96, 241, 520, 606
PspPI GGNCC 3 cut(s) 295, 519, 730
PsuI RGATCY 3 cut(s) 484, 604, 749
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 1 cut(s) 669
RsaNI GTAC 1 cut(s) 668
RseI CAYNNNNRTG 2 cut(s) 708, 1045
SaqAI TTAA 1 cut(s) 384
Sau3AI GATC 3 cut(s) 484, 604, 749
Sau96I GGNCC 3 cut(s) 295, 519, 730
ScaI AGTACT 1 cut(s) 669
ScrFI CCNGG 3 cut(s) 32, 299, 930
SetI ASST 8 cut(s) 190, 320, 437, 549, 566, 600, 788, 856
SfaNI GCATC 4 cut(s) 315, 544, 826, 857
SfcI CTRYAG 1 cut(s) 680
SinI GGWCC 1 cut(s) 295
SmiMI CAYNNNNRTG 2 cut(s) 708, 1045
SsiI CCGC 5 cut(s) 92, 345, 557, 602, 1029
SspMI CTAG 2 cut(s) 192, 1111
StyD4I CCNGG 3 cut(s) 30, 297, 928
TaaI ACNGT 1 cut(s) 1063
TaiI ACGT 3 cut(s) 549, 788, 856
TaqI TCGA 4 cut(s) 88, 321, 616, 953
TaqII GACCGA 1 cut(s) 124
TatI WGTACW 1 cut(s) 667
TfiI GAWTC 2 cut(s) 85, 167
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TspDTI ATGAA 3 cut(s) 180, 458, 597
TspGWI ACGGA 1 cut(s) 89
VpaK11BI GGWCC 1 cut(s) 295
XagI CCTNNNNNAGG 1 cut(s) 725
XapI RAATTY 3 cut(s) 51, 539, 687
XceI RCATGY 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 808
XmnI GAANNNNTTC 2 cut(s) 251, 1106
XspI CTAG 2 cut(s) 192, 1111
ZrmI AGTACT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.