Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Forward (+)

2855950 .. 2860799

Gene Structure 4850 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3594 bp

ATGAGGGGTGGAGGCAGAAGGAGAAAGCTGCATTTCAGCAAGATCGATTCTTTCACTTGTGGTAAATCTTCTATGAGAGATGAACATTCACAAATTGGGGGACCGGGATATTCCAGAGTGGTTTACTGCAATGAACCGGACTCTTTCGAGGCTCATATGCGAAATTATGGAGACAATTATGTTAGGAGCACAAAGTATACAGTTGCTACTTTCTTGCCAAAATCTTTGTTTGAGCAGTTCAGAAGGGTGGCCAACTTCTACTTCTTAGTCACTGGCACTTTGGCATTCACTCCTCTGGCTCCATTTACCGCTGTCAGCGCGATCATCCCTCTTATTATTGTGATTGGGGCGACCATGGTGAAAGAGGGTATCGAAGATTGGCGGCGAAAACAGCAGGATATTGAGGTGAACAATCGAAAGGTTAATGTACATTATGGTAATGGGGTTTTCGATTATACTGCTTGGAGGAATCTGAGAGTGGGAGATATAGTGAGAGTAGAAAAAGATGAATTCTTTCCGACAGACCTCCTCTTGCTTTCATCCAGTTATGACGATGCAATCTGCTATGTTGAGACCATGAATCTTGATGGGGAGACAAATTTAAAGTTAAAACAAGCATTGGAAGTAACTTCGTTCTTGCAGGAGGACTCTAAGTTCAACGATTTTAACGCCATTGTTAAATGTGAAGACCCAAATGCAAATTTGTACTCGTTTGTTGGAACTATGGAGTTTGACAAGCAACAGTTTCCCCTCTCTCCTCAACAGCTTCTCCTCAGAGACTCTAAACTAAGAAATACAGACTACATATATGGAGCTGTTATCTTCACTGGTCCCGACACAAAGGTTATTCAAAATTCTACTGCCCCTCCTTCAAAAAGAAGCAGAGTTGAGAAGAAGATGGATAAAATTATCTACTTCTTGTTCTGTGTTTTATTCACAATGGCATTTGTTGGGTCGATTTACTTTGGGATTGCAACAAAAGATGACCTAAACAATGGGATCATGAAAAGGTGGTATCTCAGGCCAGATAATTCTAGAATTTTCTTTGATGCTAAAAGAGCTCCGTATGCAGCAATTTACCACTTTTTGACAGCTCTAATGTTGTATGGCTACTTTATCCCCATCTCCTTGTATGTGTCAATAGAAATTGTCAAAGTTCTTCAGAGCATATTCATCAATCAAGATATTCACATGTACTATGAGGAATCTGACAAACCAGCACATGCCCGTACCTCAAACTTGAACGAGGAACTTGGCCAAGTTGACACGATTCTGTCTGATAAGACTGGAACTCTGACCTGCAATTCAATGGAGTTTATTAAATGTTCTGTGGCCGGAACAGCTTATGGCCGAGGATATACTGAGGTTGAGAGGGCTATGGGTAGGAGAAATGGTTCACCTTTGGTTCATCAACATTTAAGTGGTGGGGACAATCTCAAGGACTCTACTGATGGCAAGGCACCCATCAAGGGCTTTAATTTTAAAGACGAAAGGGTCATGAATGGAAACTGGGTTAATGAGCCTAATGCAGAATACATCCAGAAGTTTTTCAGTTTATTAGCAATCTGTCATACGGCAATTCCGGAAGTTGATGAAGCTACTGGGAATATTTCATATGAGGCTGAATCTCCTGATGAAGCAGCATTTGTGATTGCAGCAAGAGAACTTGGTTTTGAATTCTACAAAAGGACACAAACAACCATATCACTGCGAGAGTTAGATCCAGTGTCCGGCAAGAAGGTTGAGAGGACGTATAGCCTTCTCAATGTTTTAGAGTTTAACAGCACAAGGAAGCGAATGTCTGTGATTGTCAGAAGTGAGGGAGGAAAAATACTATTACTATCCAAAGGTGCCGACAATGTTATGCTTGAGAGGCTTGCCAGAAATGGTTCGGATTTTGAAGAAGAGACCATGGAGCATCTAAATGAGTATGCTGATGCAGGGCTGAGGACCTTGATTCTTGCCTACCGTGAACTTGACGAAGACGAATACAAAGAGTTCAATCAAAATTTTATCAAGGCCAAGAATTCTGTTAGTGCAGAACGTGAAACAGTCATTGATGAAGTAACAGAAAAGATTGAGAAAGATCTAATTCTTCTTGGTGCAACTGCTGTTGAGGACAAACTACAAAATGGGGTTCCTGATTGCATTGACAAGCTTGCCCAAGCAGGAATTAAGATTTGGGTTTTGACTGGAGACAAGATGGAGACTGCCATCAATATCGGGTTTGCATGTAGCTTGCTTAGACAAGGAATGAAGCAAATTGTAATCACTTTGGAGTCTCCGGAGATTAAAGTGTTGGAAAAGGAAGGAGAAAAGGAGGCCATCGCCACGGCATCAAAAGGAAGTGTTCTCAATCAGATAAATAGGGGAAAAGCTCAGCTTACAGCGTCGAGCAGGAACCCTGAGGCATTTGCATTGATCATTGATGGAAAATCACTTGCTTACGCTTTGGAGGACGATGTAAAGAATTTGTTTCTAAATCTTGCAATTGGCTGTGCATCTGTTATTTGCTGCCGTTCATCACCTAAACAGAAGGCACTGGTCACTAGACTGGTAAAATCTGGAACTGGGAAAACAACACTAGCGATTGGTGATGGAGCCAATGATGTGGGCATGCTCCAAGAAGCAGATATCGGGGTTGGAATTAGTGGTGTTGAAGGAATGCAGGCGGTCATGTCAAGCGATATTGCTATTGCACAGTTTCAATACTTGGAGCGTCTACTGCTTGTGCACGGGCATTGGTGTTACAGAAGGATCTCATCAATGATTTGCTACTTCTTCTACAAGAACATTGCATTTGGTTTCACTCTCTTCTTATATGAGGCACACACATCCTTCTCTGGAACGCCTGCATACAACGATTGGTTTTTGTCACTTTACAACGTATTCTTCTCTTCACTTCCGGTGGTTGCTATGGGAGTTTTCGACCAGGATGTATCTGCGCGGTTCTGTTTCAAGTTTCCTCTATTATACCAAGAAGGGGTGCAAAATATTCTCTTCAGCTGGCGTAGAATATTCGGATGGATGTTGAATGGGTTTACAACGGCCGCGATTATCTTCTTCTTCTGCACGAAAGCGCTAGCACACCAGGCCTTCAACAGCGAGGGCAAAACTGCCGGAAGGGATATCCTCGGGGCAACAATGTACACATGCACAGTTTGGGTTGTGAACTTGCAGATGGCACTTGCCATCAGTTACTTCACCTTGATACAGCACCTCTTCATCTGGGGTTCAATTGCACTATGGTATATCTTCCTCTTGGCTTACGGTGCCATGTCACCTTCACTCTCAACCACTGCCTACAAAATCTTCATCGAAGCCCTCGCCCCCACGCCTTCTTTCTGGCTCATCACAATCTTCGTGCCAGTCTCCGGTCTAATCCTATTCTTCACATACTCAGCCGTTCAAATGCGTTTCTTCCCTATGTACCATAGAACAATACAATGGATACGGTACGAGGGAACATCAAATGATCCCAAATTGTGTGACATGGTGCGGCAGAGATCGTTGCGGCCGCAGACTGTTGGTTTCACGGCGCGATTGGCGGCGAGGGCCAACCGTATAAAAGATAGACACCATAACCGTAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1198

Amino Acids

135.27

Weight (kDa)

7.24

Isoelectric Point (pI)

36.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	41 - 108	2.6e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	520 - 710	1.7e-19	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	892 - 1142	2.1e-85	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1493
Acc36I	ACCTGC	1 cut(s)	1307
AccB1I	GGYRCC	3 cut(s)	1459, 1850, 3272
AccI	GTMKAC	2 cut(s)	197, 2722
AccII	CGCG	4 cut(s)	320, 2947, 3053, 3541
AccIII	TCCGGA	2 cut(s)	1582, 2284
AciI	CCGC	9 cut(s)	309, 382, 2672, 2947, 3051, 3499, 3514, 3518, 3548
AclWI	GGATC	4 cut(s)	1007, 1715, 2765, 3470
AcoI	YGGCCR	6 cut(s)	249, 1255, 1332, 1348, 3048, 3515
AcsI	RAATTY	9 cut(s)	509, 598, 700, 853, 1038, 1676, 2008, 2026, 2470
AcuI	CTGAAG	2 cut(s)	1145, 2986
AfaI	GTAC	7 cut(s)	429, 707, 1196, 1231, 3149, 3431, 3458
AfeI	AGCGCT	1 cut(s)	3081
AfiI	CCNNNNNNNGG	4 cut(s)	1469, 1730, 2983, 3374
AflIII	ACRYGT	1 cut(s)	1191
AjnI	CCWGG	2 cut(s)	2931, 3090
AjuI	GAANNNNNNNTTGG	4 cut(s)	245, 277, 2164, 2196
AloI	GAACNNNNNNTCC	4 cut(s)	638, 670, 1378, 1410
Alw21I	GWGCWC	3 cut(s)	191, 1063, 2736
Alw26I	GTCTC	9 cut(s)	165, 566, 587, 771, 1901, 2190, 2201, 2286, 3376
Alw44I	GTGCAC	1 cut(s)	2732
AlwI	GGATC	4 cut(s)	1007, 1715, 2765, 3470
Ama87I	CYCGRG	1 cut(s)	3134
Aor13HI	TCCGGA	2 cut(s)	1582, 2284
Aor51HI	AGCGCT	1 cut(s)	3081
ApaLI	GTGCAC	1 cut(s)	2732
ApeKI	GCWGC	5 cut(s)	28, 1070, 1640, 1655, 2514
ApoI	RAATTY	9 cut(s)	509, 598, 700, 853, 1038, 1676, 2008, 2026, 2470
Asp700I	GAANNNNTTC	4 cut(s)	513, 1393, 1547, 1888
AspLEI	GCGC	4 cut(s)	320, 2947, 3082, 3541
AspS9I	GGNCC	4 cut(s)	101, 830, 1950, 3555
AsuC2I	CCSGG	1 cut(s)	105
AsuHPI	GGTGA	7 cut(s)	370, 418, 1389, 2517, 2606, 3196, 3273
AsuNHI	GCTAGC	1 cut(s)	3082
AvaI	CYCGRG	1 cut(s)	3134
AvaII	GGWCC	3 cut(s)	101, 830, 1950
AxyI	CCTNAGG	1 cut(s)	2406
BaeGI	GKGCMC	1 cut(s)	2736
BalI	TGGCCA	2 cut(s)	251, 1257
BanI	GGYRCC	3 cut(s)	1459, 1850, 3272
BanII	GRGCYC	1 cut(s)	1063
BbsI	GAAGAC	2 cut(s)	693, 1989
Bbv12I	GWGCWC	3 cut(s)	191, 1063, 2736
BbvCI	CCTCAGC	1 cut(s)	1946
BbvI	GCAGC	5 cut(s)	15, 1082, 1652, 1667, 2501
BceAI	ACGGC	6 cut(s)	1590, 2349, 2502, 3063, 3389, 3552
BcgI	CGANNNNNNTGC	2 cut(s)	440, 474
BciT130I	CCWGG	2 cut(s)	2933, 3092
BciVI	GTATCC	1 cut(s)	3444
BclI	TGATCA	1 cut(s)	2421
BcnI	CCSGG	1 cut(s)	105
BcoDI	GTCTC	9 cut(s)	165, 566, 587, 771, 1901, 2190, 2201, 2286, 3376
BfaI	CTAG	4 cut(s)	1035, 2550, 2585, 3083
BfoI	RGCGCY	1 cut(s)	3083
BfuAI	ACCTGC	1 cut(s)	1307
BfuI	GTATCC	1 cut(s)	3444
BglII	AGATCT	1 cut(s)	2086
BlpI	GCTNAGC	1 cut(s)	2379
Bme1390I	CCNGG	3 cut(s)	105, 2933, 3092
Bme18I	GGWCC	3 cut(s)	101, 830, 1950
BmeT110I	CYCGRG	1 cut(s)	3134
BmgT120I	GGNCC	4 cut(s)	101, 830, 1950, 3555
BmiI	GGNNCC	9 cut(s)	102, 300, 832, 1461, 1852, 2139, 2402, 2602, 3274
BmrFI	CCNGG	3 cut(s)	105, 2933, 3092
BmrI	ACTGGG	3 cut(s)	1519, 1611, 2580
BmsI	GCATC	6 cut(s)	544, 1039, 1927, 1927, 2345, 2510
BmtI	GCTAGC	1 cut(s)	3086
BmuI	ACTGGG	3 cut(s)	1519, 1611, 2580
BpiI	GAAGAC	2 cut(s)	693, 1989
BpmI	CTGGAG	1 cut(s)	2214
Bpu10I	CCTNAGC	1 cut(s)	1946
Bpu1102I	GCTNAGC	1 cut(s)	2379
BpuEI	CTTGAG	2 cut(s)	1421, 1889
BpuMI	CCSGG	1 cut(s)	105
Bsa29I	ATCGAT	1 cut(s)	45
BsaI	GGTCTC	2 cut(s)	566, 1901
BsaJI	CCNNGG	5 cut(s)	354, 1351, 1911, 2331, 3133
BsaWI	WCCGGW	5 cut(s)	136, 1582, 2284, 2905, 3374
BsaXI	ACNNNNNCTCC	8 cut(s)	474, 504, 753, 783, 850, 880, 1378, 1408
Bsc4I	CCNNNNNNNGG	4 cut(s)	1469, 1730, 2983, 3374
Bse21I	CCTNAGG	1 cut(s)	2406
Bse3DI	GCAATG	2 cut(s)	136, 2793
BseAI	TCCGGA	2 cut(s)	1582, 2284
BseBI	CCWGG	2 cut(s)	2933, 3092
BseCI	ATCGAT	1 cut(s)	45
BseDI	CCNNGG	5 cut(s)	354, 1351, 1911, 2331, 3133
BseGI	GGATG	7 cut(s)	324, 539, 1536, 2834, 2941, 3029, 3033
BseLI	CCNNNNNNNGG	4 cut(s)	1469, 1730, 2983, 3374
BseMI	GCAATG	2 cut(s)	136, 2793
BseMII	CTCAG	8 cut(s)	464, 787, 1033, 1353, 1937, 2393, 2397, 3414
BseRI	GAGGAG	4 cut(s)	282, 518, 747, 761
BseSI	GKGCMC	1 cut(s)	2736
BseX3I	CGGCCG	2 cut(s)	3048, 3515
BseXI	GCAGC	5 cut(s)	15, 1082, 1652, 1667, 2501
BsgI	GTGCAG	2 cut(s)	2058, 3055
Bsh1236I	CGCG	4 cut(s)	320, 2947, 3053, 3541
Bsh1285I	CGRYCG	2 cut(s)	3051, 3518
BshNI	GGYRCC	3 cut(s)	1459, 1850, 3272
BshVI	ATCGAT	1 cut(s)	45
BsiEI	CGRYCG	2 cut(s)	3051, 3518
BsiHKAI	GWGCWC	3 cut(s)	191, 1063, 2736
BsiHKCI	CYCGRG	1 cut(s)	3134
BsiSI	CCGG	9 cut(s)	104, 137, 1335, 1583, 1731, 2285, 2906, 3120, 3375
BslFI	GGGAC	3 cut(s)	114, 816, 1442
BslI	CCNNNNNNNGG	4 cut(s)	1469, 1730, 2983, 3374
BsmAI	GTCTC	9 cut(s)	165, 566, 587, 771, 1901, 2190, 2201, 2286, 3376
BsmFI	GGGAC	3 cut(s)	114, 816, 1442
BsmI	GAATGC	2 cut(s)	284, 2670
Bso31I	GGTCTC	2 cut(s)	566, 1901
BsoBI	CYCGRG	1 cut(s)	3134
Bsp1286I	GDGCHC	3 cut(s)	191, 1063, 2736
Bsp13I	TCCGGA	2 cut(s)	1582, 2284
Bsp1407I	TGTACA	2 cut(s)	427, 3147
Bsp143I	GATC	9 cut(s)	42, 321, 999, 1720, 2086, 2421, 2757, 3475, 3506
Bsp1720I	GCTNAGC	1 cut(s)	2379
Bsp19I	CCATGG	2 cut(s)	354, 1911
BspACI	CCGC	9 cut(s)	309, 382, 2672, 2947, 3051, 3499, 3514, 3518, 3548
BspCNI	CTCAG	8 cut(s)	465, 786, 1032, 1354, 1938, 2392, 2398, 3413
BspDI	ATCGAT	1 cut(s)	45
BspEI	TCCGGA	2 cut(s)	1582, 2284
BspFNI	CGCG	4 cut(s)	320, 2947, 3053, 3541
BspHI	TCATGA	2 cut(s)	1002, 1497
BspLI	GGNNCC	9 cut(s)	102, 300, 832, 1461, 1852, 2139, 2402, 2602, 3274
BspMI	ACCTGC	1 cut(s)	1307
BspOI	GCTAGC	1 cut(s)	3086
BspPI	GGATC	4 cut(s)	1007, 1715, 2765, 3470
BspT107I	GGYRCC	3 cut(s)	1459, 1850, 3272
BspTNI	GGTCTC	2 cut(s)	566, 1901
BsrDI	GCAATG	2 cut(s)	136, 2793
BsrGI	TGTACA	2 cut(s)	427, 3147
BssECI	CCNNGG	5 cut(s)	354, 1351, 1911, 2331, 3133
BssMI	GATC	9 cut(s)	42, 321, 999, 1720, 2086, 2421, 2757, 3475, 3506
BssNAI	GTATAC	1 cut(s)	198
BssT1I	CCWWGG	2 cut(s)	354, 1911
Bst1107I	GTATAC	1 cut(s)	198
Bst2UI	CCWGG	2 cut(s)	2933, 3092
Bst6I	CTCTTC	5 cut(s)	1899, 2819, 2902, 3005, 3227
BstAUI	TGTACA	2 cut(s)	427, 3147
BstC8I	GCNNGC	8 cut(s)	1878, 2160, 2240, 2618, 2670, 2853, 3008, 3084
BstDSI	CCRYGG	3 cut(s)	354, 1911, 2331
BstF5I	GGATG	7 cut(s)	324, 539, 1536, 2834, 2941, 3029, 3033
BstFNI	CGCG	4 cut(s)	320, 2947, 3053, 3541
BstH2I	RGCGCY	1 cut(s)	3083
BstHHI	GCGC	4 cut(s)	320, 2947, 3082, 3541
BstKTI	GATC	9 cut(s)	45, 324, 1002, 1723, 2089, 2424, 2760, 3478, 3509
BstMAI	GTCTC	9 cut(s)	165, 566, 587, 771, 1901, 2190, 2201, 2286, 3376
BstMBI	GATC	9 cut(s)	42, 321, 999, 1720, 2086, 2421, 2757, 3475, 3506
BstMCI	CGRYCG	2 cut(s)	3051, 3518
BstNI	CCWGG	2 cut(s)	2933, 3092
BstNSI	RCATGY	5 cut(s)	1195, 1226, 2235, 2620, 3156
BstSCI	CCNGG	3 cut(s)	103, 2931, 3090
BstSLI	GKGCMC	1 cut(s)	2736
BstUI	CGCG	4 cut(s)	320, 2947, 3053, 3541
BstV1I	GCAGC	5 cut(s)	15, 1082, 1652, 1667, 2501
BstV2I	GAAGAC	2 cut(s)	693, 1989
BstX2I	RGATCY	3 cut(s)	1720, 2086, 2757
BstXI	CCANNNNNNTGG	1 cut(s)	2611
BstYI	RGATCY	3 cut(s)	1720, 2086, 2757
BstZ17I	GTATAC	1 cut(s)	198
BstZI	CGGCCG	2 cut(s)	3048, 3515
Bsu15I	ATCGAT	1 cut(s)	45
Bsu36I	CCTNAGG	1 cut(s)	2406
BsuI	GTATCC	1 cut(s)	3444
BsuTUI	ATCGAT	1 cut(s)	45
BtgI	CCRYGG	3 cut(s)	354, 1911, 2331
BtgZI	GCGATG	1 cut(s)	2311
BtsCI	GGATG	7 cut(s)	324, 539, 1536, 2834, 2941, 3029, 3033
BtsI	GCAGTG	2 cut(s)	1706, 3297
BtsIMutI	CAGTG	6 cut(s)	270, 825, 1706, 1731, 2540, 3297
BveI	ACCTGC	1 cut(s)	1307
Cac8I	GCNNGC	8 cut(s)	1878, 2160, 2240, 2618, 2670, 2853, 3008, 3084
CciI	TCATGA	2 cut(s)	1002, 1497
CciNI	GCGGCCGC	1 cut(s)	3515
CfoI	GCGC	4 cut(s)	320, 2947, 3082, 3541
Cfr13I	GGNCC	4 cut(s)	101, 830, 1950, 3555
ClaI	ATCGAT	1 cut(s)	45
CseI	GACGC	2 cut(s)	2379, 2708
Csp6I	GTAC	7 cut(s)	428, 706, 1195, 1230, 3148, 3430, 3457
CviQI	GTAC	7 cut(s)	428, 706, 1195, 1230, 3148, 3430, 3457
DpnI	GATC	9 cut(s)	44, 323, 1001, 1722, 2088, 2423, 2759, 3477, 3508
DpnII	GATC	9 cut(s)	42, 321, 999, 1720, 2086, 2421, 2757, 3475, 3506
DraI	TTTAAA	2 cut(s)	603, 1483
DrdI	GACNNNNNNGTC	1 cut(s)	1493
DseDI	GACNNNNNNGTC	1 cut(s)	1493
EaeI	YGGCCR	6 cut(s)	249, 1255, 1332, 1348, 3048, 3515
EagI	CGGCCG	2 cut(s)	3048, 3515
Eam1104I	CTCTTC	5 cut(s)	1899, 2819, 2902, 3005, 3227
EarI	CTCTTC	5 cut(s)	1899, 2819, 2902, 3005, 3227
Ecl136II	GAGCTC	1 cut(s)	1061
EclXI	CGGCCG	2 cut(s)	3048, 3515
Eco130I	CCWWGG	2 cut(s)	354, 1911
Eco147I	AGGCCT	1 cut(s)	3095
Eco24I	GRGCYC	1 cut(s)	1063
Eco31I	GGTCTC	2 cut(s)	566, 1901
Eco32I	GATATC	2 cut(s)	2635, 3130
Eco47I	GGWCC	3 cut(s)	101, 830, 1950
Eco47III	AGCGCT	1 cut(s)	3081
Eco52I	CGGCCG	2 cut(s)	3048, 3515
Eco53kI	GAGCTC	1 cut(s)	1061
Eco57I	CTGAAG	2 cut(s)	1145, 2986
Eco81I	CCTNAGG	1 cut(s)	2406
Eco88I	CYCGRG	1 cut(s)	3134
EcoICRI	GAGCTC	1 cut(s)	1061
EcoO109I	RGGNCCY	1 cut(s)	1950
EcoRI	GAATTC	3 cut(s)	509, 1676, 2026
EcoRII	CCWGG	2 cut(s)	2931, 3090
EcoRV	GATATC	2 cut(s)	2635, 3130
EcoT14I	CCWWGG	2 cut(s)	354, 1911
EcoT38I	GRGCYC	1 cut(s)	1063
ErhI	CCWWGG	2 cut(s)	354, 1911
FalI	AAGNNNNNCTT	2 cut(s)	2367, 2399
FaqI	GGGAC	3 cut(s)	114, 816, 1442
FauNDI	CATATG	2 cut(s)	156, 1615
FbaI	TGATCA	1 cut(s)	2421
FblI	GTMKAC	2 cut(s)	197, 2722
FokI	GGATG	7 cut(s)	311, 526, 1523, 2821, 2948, 3036, 3040
FriOI	GRGCYC	1 cut(s)	1063
FspBI	CTAG	4 cut(s)	1035, 2550, 2585, 3083
GlaI	GCGC	4 cut(s)	319, 2946, 3081, 3540
GsuI	CTGGAG	1 cut(s)	2214
HaeII	RGCGCY	1 cut(s)	3083
HapII	CCGG	9 cut(s)	104, 137, 1335, 1583, 1731, 2285, 2906, 3120, 3375
HgaI	GACGC	2 cut(s)	2379, 2708
HhaI	GCGC	4 cut(s)	320, 2947, 3082, 3541
Hin6I	GCGC	4 cut(s)	318, 2945, 3080, 3539
HinP1I	GCGC	4 cut(s)	318, 2945, 3080, 3539
HincII	GTYRAC	1 cut(s)	1264
HindII	GTYRAC	1 cut(s)	1264
HindIII	AAGCTT	1 cut(s)	2156
HpaII	CCGG	9 cut(s)	104, 137, 1335, 1583, 1731, 2285, 2906, 3120, 3375
HphI	GGTGA	7 cut(s)	370, 418, 1389, 2517, 2606, 3196, 3273
Hpy99I	CGWCG	1 cut(s)	2395
HpyCH4IV	ACGT	3 cut(s)	1751, 2044, 2886
HpySE526I	ACGT	3 cut(s)	1751, 2044, 2886
HspAI	GCGC	4 cut(s)	318, 2945, 3080, 3539
Kpn2I	TCCGGA	2 cut(s)	1582, 2284
Ksp22I	TGATCA	1 cut(s)	2421
Kzo9I	GATC	9 cut(s)	42, 321, 999, 1720, 2086, 2421, 2757, 3475, 3506
LmnI	GCTCC	8 cut(s)	186, 304, 812, 1066, 1915, 2600, 2625, 2716
Lsp1109I	GCAGC	5 cut(s)	15, 1082, 1652, 1667, 2501
LweI	GCATC	6 cut(s)	544, 1039, 1927, 1927, 2345, 2510
MaeI	CTAG	4 cut(s)	1035, 2550, 2585, 3083
MaeII	ACGT	3 cut(s)	1751, 2044, 2886
MaeIII	GTNAC	9 cut(s)	268, 625, 2065, 2545, 2747, 2874, 3197, 3279, 3488
MalI	GATC	9 cut(s)	44, 323, 1001, 1722, 2088, 2423, 2759, 3477, 3508
MboI	GATC	9 cut(s)	42, 321, 999, 1720, 2086, 2421, 2757, 3475, 3506
MfeI	CAATTG	2 cut(s)	2490, 3237
MflI	RGATCY	3 cut(s)	1720, 2086, 2757
MhlI	GDGCHC	3 cut(s)	191, 1063, 2736
MlsI	TGGCCA	2 cut(s)	251, 1257
MluNI	TGGCCA	2 cut(s)	251, 1257
MlyI	GAGTC	5 cut(s)	134, 641, 773, 1436, 2288
MmeI	TCCRAC	4 cut(s)	542, 697, 2280, 2623
Mox20I	TGGCCA	2 cut(s)	251, 1257
MroI	TCCGGA	2 cut(s)	1582, 2284
MroXI	GAANNNNTTC	4 cut(s)	513, 1393, 1547, 1888
MscI	TGGCCA	2 cut(s)	251, 1257
MslI	CAYNNNNRTG	2 cut(s)	1419, 1923
Msp20I	TGGCCA	2 cut(s)	251, 1257
MspA1I	CMGCKG	2 cut(s)	311, 3006
MspI	CCGG	9 cut(s)	104, 137, 1335, 1583, 1731, 2285, 2906, 3120, 3375
MspR9I	CCNGG	3 cut(s)	105, 2933, 3092
MunI	CAATTG	2 cut(s)	2490, 3237
Mva1269I	GAATGC	2 cut(s)	284, 2670
MvaI	CCWGG	2 cut(s)	2933, 3092
MvnI	CGCG	4 cut(s)	320, 2947, 3053, 3541
NciI	CCSGG	1 cut(s)	105
NcoI	CCATGG	2 cut(s)	354, 1911
NdeI	CATATG	2 cut(s)	156, 1615
NdeII	GATC	9 cut(s)	42, 321, 999, 1720, 2086, 2421, 2757, 3475, 3506
NheI	GCTAGC	1 cut(s)	3082
NlaIV	GGNNCC	9 cut(s)	102, 300, 832, 1461, 1852, 2139, 2402, 2602, 3274
NmeAIII	GCCGAG	1 cut(s)	1376
NmuCI	GTSAC	5 cut(s)	268, 2545, 2874, 3279, 3488
NotI	GCGGCCGC	1 cut(s)	3515
NspI	RCATGY	5 cut(s)	1195, 1226, 2235, 2620, 3156
PaeI	GCATGC	1 cut(s)	2620
PagI	TCATGA	2 cut(s)	1002, 1497
PceI	AGGCCT	1 cut(s)	3095
PciI	ACATGT	1 cut(s)	1191
PctI	GAATGC	2 cut(s)	284, 2670
PdmI	GAANNNNTTC	4 cut(s)	513, 1393, 1547, 1888
PfeI	GAWTC	7 cut(s)	47, 469, 580, 1205, 1270, 1625, 1957
PleI	GAGTC	5 cut(s)	134, 641, 773, 1436, 2287
PpsI	GAGTC	5 cut(s)	134, 641, 773, 1436, 2287
PpuMI	RGGWCCY	1 cut(s)	1950
PscI	ACATGT	1 cut(s)	1191
Psp124BI	GAGCTC	1 cut(s)	1063
Psp5II	RGGWCCY	1 cut(s)	1950
Psp6I	CCWGG	2 cut(s)	2931, 3090
PspGI	CCWGG	2 cut(s)	2931, 3090
PspN4I	GGNNCC	9 cut(s)	102, 300, 832, 1461, 1852, 2139, 2402, 2602, 3274
PspPI	GGNCC	4 cut(s)	101, 830, 1950, 3555
PspPPI	RGGWCCY	1 cut(s)	1950
PsuI	RGATCY	3 cut(s)	1720, 2086, 2757
PvuII	CAGCTG	1 cut(s)	3006
RsaI	GTAC	7 cut(s)	429, 707, 1196, 1231, 3149, 3431, 3458
RsaNI	GTAC	7 cut(s)	428, 706, 1195, 1230, 3148, 3430, 3457
RseI	CAYNNNNRTG	2 cut(s)	1419, 1923
SacI	GAGCTC	1 cut(s)	1063
Sau3AI	GATC	9 cut(s)	42, 321, 999, 1720, 2086, 2421, 2757, 3475, 3506
Sau96I	GGNCC	4 cut(s)	101, 830, 1950, 3555
SchI	GAGTC	5 cut(s)	134, 641, 773, 1436, 2288
ScrFI	CCNGG	3 cut(s)	105, 2933, 3092
SduI	GDGCHC	3 cut(s)	191, 1063, 2736
SfaNI	GCATC	6 cut(s)	544, 1039, 1927, 1927, 2345, 2510
SinI	GGWCC	3 cut(s)	101, 830, 1950
SmiMI	CAYNNNNRTG	2 cut(s)	1419, 1923
SmlI	CTYRAG	2 cut(s)	1436, 1868
SmoI	CTYRAG	2 cut(s)	1436, 1868
SphI	GCATGC	1 cut(s)	2620
SseBI	AGGCCT	1 cut(s)	3095
SsiI	CCGC	9 cut(s)	309, 382, 2672, 2947, 3051, 3499, 3514, 3518, 3548
SspI	AATATT	3 cut(s)	1609, 2995, 3018
SspMI	CTAG	4 cut(s)	1035, 2550, 2585, 3083
SstI	GAGCTC	1 cut(s)	1063
StuI	AGGCCT	1 cut(s)	3095
StyD4I	CCNGG	3 cut(s)	103, 2931, 3090
StyI	CCWWGG	2 cut(s)	354, 1911
TaiI	ACGT	3 cut(s)	1754, 2047, 2889
TaqI	TCGA	9 cut(s)	45, 147, 372, 415, 450, 956, 2393, 2928, 3318
TatI	WGTACW	4 cut(s)	427, 705, 1194, 3147
TauI	GCSGC	6 cut(s)	385, 3053, 3502, 3517, 3520, 3551
TfiI	GAWTC	7 cut(s)	47, 469, 580, 1205, 1270, 1625, 1957
TscAI	CASTG	6 cut(s)	277, 832, 1713, 1731, 2547, 3304
TseFI	GTSAC	5 cut(s)	268, 2545, 2874, 3279, 3488
TseI	GCWGC	5 cut(s)	28, 1070, 1640, 1655, 2514
Tsp45I	GTSAC	5 cut(s)	268, 2545, 2874, 3279, 3488
TspGWI	ACGGA	1 cut(s)	1053
TspRI	CASTG	6 cut(s)	277, 832, 1713, 1731, 2547, 3304
VneI	GTGCAC	1 cut(s)	2732
VpaK11BI	GGWCC	3 cut(s)	101, 830, 1950
XapI	RAATTY	9 cut(s)	509, 598, 700, 853, 1038, 1676, 2008, 2026, 2470
XbaI	TCTAGA	1 cut(s)	1034
XceI	RCATGY	5 cut(s)	1195, 1226, 2235, 2620, 3156
XmiI	GTMKAC	2 cut(s)	197, 2722
XmnI	GAANNNNTTC	4 cut(s)	513, 1393, 1547, 1888
XspI	CTAG	4 cut(s)	1035, 2550, 2585, 3083

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.