pycom16g06100

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002644
Physical Location & Seq
Reverse (-)
3894670 .. 3895543
874 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGAAGGCAGGAGGAGGACCAGTTGTTAAGGTTACTGAAGCCTCGAAACCGATCCCGAAAATGAAGTCGAGCAGAGTACTCGCTGTTCTAGACTTCACTCTTCGCGTCGTTGCAGTGATCGGAACATTAGCTAGTGCCATAGCTATGGGAACCAGCAGGGAAACACTTCCCTTCGCCACACGGTTTGTTCGCTTCAGGGCTCGGTACAAGGATCTTCCCACGTTAACGTTCTTTGTGATGACCAATTCGATCGTATGCGTCTACCTTGTGCTTTCCCTTCCGGTATCTATTGTCCATATCATCTGGACTGCAGCAGTAAACAGTAGGATCATCTTGATCATCTCGGACACGGTGATGATGGGGCTTGTAACTGCCGGAGCTTCAGCAGCTACAGCCATAGTGTACCTGGCGCACTACGGGAACCCAGGCACCAACTGGTTCGCATTCTGTCAGCAGTTCAACAGCTTCTGTGGGCGCATTTCCGGGTCTCTCATTGGCTCCTTCATCGCCATAACTGTACTTCTACTGCTAATCATCATGTCATCTGTGGCAATTTCTCGACGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

189

Amino Acids

20.22

Weight (kDa)

10.53

Isoelectric Point (pI)

26.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 25 - 172 7e-44 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 428
AccI GTMKAC 1 cut(s) 261
AccII CGCG 1 cut(s) 105
AclI AACGTT 1 cut(s) 227
AclWI GGATC 3 cut(s) 46, 219, 335
AcuI CTGAAG 3 cut(s) 57, 178, 366
AfaI GTAC 4 cut(s) 78, 206, 404, 519
AgsI TTSAA 1 cut(s) 460
AjnI CCWGG 2 cut(s) 405, 424
AluBI AGCT 5 cut(s) 131, 143, 380, 389, 465
AluI AGCT 5 cut(s) 131, 143, 380, 389, 465
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 3 cut(s) 46, 219, 335
AlwNI CAGNNNCTG 1 cut(s) 468
ApeKI GCWGC 2 cut(s) 311, 386
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
Asp700I GAANNNNTTC 1 cut(s) 165
AspLEI GCGC 2 cut(s) 412, 477
AspS9I GGNCC 1 cut(s) 17
AsuC2I CCSGG 1 cut(s) 484
AsuHPI GGTGA 1 cut(s) 364
AvaII GGWCC 1 cut(s) 17
BanI GGYRCC 1 cut(s) 428
BanII GRGCYC 1 cut(s) 202
BbvI GCAGC 2 cut(s) 323, 398
BccI CCATC 1 cut(s) 352
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 2 cut(s) 407, 426
BclI TGATCA 1 cut(s) 336
BcnI CCSGG 1 cut(s) 484
BcoDI GTCTC 1 cut(s) 492
BfaI CTAG 2 cut(s) 89, 132
BfmI CTRYAG 2 cut(s) 309, 390
BisI GCNGC 2 cut(s) 312, 387
BlsI GCNGC 2 cut(s) 313, 388
BmcAI AGTACT 1 cut(s) 78
Bme1390I CCNGG 3 cut(s) 407, 426, 484
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 4 cut(s) 151, 422, 430, 499
BmrFI CCNGG 3 cut(s) 407, 426, 484
BpuMI CCSGG 1 cut(s) 484
BsaI GGTCTC 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 424
BsaWI WCCGGW 1 cut(s) 280
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bse1I ACTGG 2 cut(s) 20, 440
BseBI CCWGG 2 cut(s) 407, 426
BseDI CCNNGG 1 cut(s) 424
BseNI ACTGG 2 cut(s) 20, 440
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 323, 398
Bsh1236I CGCG 1 cut(s) 105
Bsh1285I CGRYCG 1 cut(s) 252
BshNI GGYRCC 1 cut(s) 428
BsiEI CGRYCG 1 cut(s) 252
BsiSI CCGG 3 cut(s) 281, 375, 483
BsmAI GTCTC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 443
Bso31I GGTCTC 1 cut(s) 492
Bsp1286I GDGCHC 1 cut(s) 202
Bsp143I GATC 6 cut(s) 51, 117, 211, 249, 327, 336
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 4 cut(s) 151, 422, 430, 499
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 3 cut(s) 46, 219, 335
BspT107I GGYRCC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 492
BsrI ACTGG 2 cut(s) 20, 440
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 6 cut(s) 51, 117, 211, 249, 327, 336
Bst2UI CCWGG 2 cut(s) 407, 426
Bst4CI ACNGT 4 cut(s) 183, 323, 352, 517
Bst6I CTCTTC 1 cut(s) 105
BstFNI CGCG 1 cut(s) 105
BstHHI GCGC 2 cut(s) 412, 477
BstKTI GATC 6 cut(s) 54, 120, 214, 252, 330, 339
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 6 cut(s) 51, 117, 211, 249, 327, 336
BstMCI CGRYCG 1 cut(s) 252
BstMWI GCNNNNNNNGC 2 cut(s) 386, 392
BstNI CCWGG 2 cut(s) 407, 426
BstSCI CCNGG 3 cut(s) 405, 424, 482
BstSFI CTRYAG 2 cut(s) 309, 390
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 323, 398
BstX2I RGATCY 1 cut(s) 211
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 1 cut(s) 211
BtgZI GCGATG 1 cut(s) 490
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 120
CaiI CAGNNNCTG 1 cut(s) 468
CfoI GCGC 2 cut(s) 412, 477
Cfr13I GGNCC 1 cut(s) 17
CseI GACGC 2 cut(s) 94, 247
Csp6I GTAC 4 cut(s) 77, 205, 403, 518
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviAII CATG 1 cut(s) 538
CviQI GTAC 4 cut(s) 77, 205, 403, 518
DpnI GATC 6 cut(s) 53, 119, 213, 251, 329, 338
DpnII GATC 6 cut(s) 51, 117, 211, 249, 327, 336
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 202
Eco31I GGTCTC 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 17
Eco57I CTGAAG 3 cut(s) 57, 178, 366
EcoRII CCWGG 2 cut(s) 405, 424
EcoT38I GRGCYC 1 cut(s) 202
FaeI CATG 1 cut(s) 541
FaiI YATR 7 cut(s) 140, 146, 256, 297, 398, 512, 539
FatI CATG 1 cut(s) 537
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 312, 387
FriOI GRGCYC 1 cut(s) 202
Fsp4HI GCNGC 2 cut(s) 312, 387
FspBI CTAG 2 cut(s) 89, 132
GlaI GCGC 2 cut(s) 411, 476
GluI GCNGC 2 cut(s) 312, 387
HapII CCGG 3 cut(s) 281, 375, 483
HgaI GACGC 2 cut(s) 94, 247
HhaI GCGC 2 cut(s) 412, 477
Hin1II CATG 1 cut(s) 541
Hin6I GCGC 2 cut(s) 410, 475
HinP1I GCGC 2 cut(s) 410, 475
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HpaI GTTAAC 1 cut(s) 225
HpaII CCGG 3 cut(s) 281, 375, 483
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 4 cut(s) 225, 262, 319, 403
Hpy188I TCNGA 2 cut(s) 122, 346
Hpy188III TCNNGA 5 cut(s) 55, 89, 304, 334, 558
Hpy8I GTNNAC 4 cut(s) 225, 262, 319, 403
Hpy99I CGWCG 2 cut(s) 110, 564
HpyAV CCTTC 3 cut(s) 181, 287, 511
HpyCH4III ACNGT 4 cut(s) 183, 323, 352, 517
HpyCH4IV ACGT 2 cut(s) 221, 227
HpyCH4V TGCA 2 cut(s) 113, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 392
HpySE526I ACGT 2 cut(s) 221, 227
Hsp92II CATG 1 cut(s) 541
HspAI GCGC 2 cut(s) 410, 475
Ksp22I TGATCA 1 cut(s) 336
KspAI GTTAAC 1 cut(s) 225
Kzo9I GATC 6 cut(s) 51, 117, 211, 249, 327, 336
LmnI GCTCC 2 cut(s) 377, 503
Lsp1109I GCAGC 2 cut(s) 323, 398
MaeI CTAG 2 cut(s) 89, 132
MaeII ACGT 2 cut(s) 221, 227
MaeIII GTNAC 2 cut(s) 31, 367
MalI GATC 6 cut(s) 53, 119, 213, 251, 329, 338
MboI GATC 6 cut(s) 51, 117, 211, 249, 327, 336
MboII GAAGA 2 cut(s) 92, 206
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 202
MluCI AATT 2 cut(s) 244, 552
MnlI CCTC 3 cut(s) 5, 8, 52
MroXI GAANNNNTTC 1 cut(s) 165
MseI TTAA 2 cut(s) 27, 224
MslI CAYNNNNRTG 2 cut(s) 143, 353
MspI CCGG 3 cut(s) 281, 375, 483
MspR9I CCNGG 3 cut(s) 407, 426, 484
Mva1269I GAATGC 1 cut(s) 443
MvaI CCWGG 2 cut(s) 407, 426
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 386, 392
NciI CCSGG 1 cut(s) 484
NdeII GATC 6 cut(s) 51, 117, 211, 249, 327, 336
NlaIII CATG 1 cut(s) 541
NlaIV GGNNCC 4 cut(s) 151, 422, 430, 499
PctI GAATGC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 165
PkrI GCNGC 2 cut(s) 313, 388
Ple19I CGATCG 1 cut(s) 252
Psp1406I AACGTT 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 405, 424
PspGI CCWGG 2 cut(s) 405, 424
PspN4I GGNNCC 4 cut(s) 151, 422, 430, 499
PspPI GGNCC 1 cut(s) 17
PsrI GAACNNNNNNTAC 2 cut(s) 69, 101
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 468
PsuI RGATCY 1 cut(s) 211
PvuI CGATCG 1 cut(s) 252
RsaI GTAC 4 cut(s) 78, 206, 404, 519
RsaNI GTAC 4 cut(s) 77, 205, 403, 518
RseI CAYNNNNRTG 2 cut(s) 143, 353
SaqAI TTAA 2 cut(s) 27, 224
SatI GCNGC 2 cut(s) 312, 387
Sau3AI GATC 6 cut(s) 51, 117, 211, 249, 327, 336
Sau96I GGNCC 1 cut(s) 17
ScaI AGTACT 1 cut(s) 78
ScrFI CCNGG 3 cut(s) 407, 426, 484
SduI GDGCHC 1 cut(s) 202
SfcI CTRYAG 2 cut(s) 309, 390
SinI GGWCC 1 cut(s) 17
SmiMI CAYNNNNRTG 2 cut(s) 143, 353
Sse9I AATT 2 cut(s) 244, 552
SspMI CTAG 2 cut(s) 89, 132
StyD4I CCNGG 3 cut(s) 405, 424, 482
TaaI ACNGT 4 cut(s) 183, 323, 352, 517
TaiI ACGT 2 cut(s) 224, 230
TaqI TCGA 4 cut(s) 44, 68, 248, 559
TasI AATT 2 cut(s) 244, 552
TatI WGTACW 2 cut(s) 76, 517
Tru1I TTAA 2 cut(s) 27, 224
Tru9I TTAA 2 cut(s) 27, 224
TscAI CASTG 1 cut(s) 120
TseI GCWGC 2 cut(s) 311, 386
TspDTI ATGAA 3 cut(s) 17, 77, 493
TspRI CASTG 1 cut(s) 120
VpaK11BI GGWCC 1 cut(s) 17
XbaI TCTAGA 1 cut(s) 88
XcmI CCANNNNNNNNNTGG 2 cut(s) 403, 432
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 165
XspI CTAG 2 cut(s) 89, 132
ZrmI AGTACT 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.