pycom16g06110

Belongs to the endosulfine family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006518
Physical Location & Seq
Forward (+)
3898046 .. 3900224
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
ATGGGTTTGAGCGTAATCTCTAGGCTTGCTTCTTCTTCTTCTTCTTCTTCAGATATCCTCTGCTTCTGCAATCGGATCCGATCTTTGATCAGTTGTCGCCAGCACCCTGAGCAAAATATGTCTGGTACAAACATGGAGGAGGTTAAGGACCAAGAGGTTGCTGATAGCACTCCTATGGATCTAGGTGATGGTGACAATGCCGGGGTAGACCCAAAAGCTTCAAATGAAAATCATGGAAATCCAATGCCTTCAGCCCAGCAGGAGGAAGCAGCAATTAAGAAAAAGTATGGAGGCATACTGCCAAGGAAGACTCCCCTAATATCCAAGGACCATGAGCGTGCTTATTTTGATTCTGCTGATTGGGCACTTGGAAAGCAAGGTGCAAAGCCGAAAGGGCCGCTTGAAGCACTCCGCCCAAAGCTGCAGCCCACACCGCACCAGCAAGGGCGTTCAAGGCGCTCAGCTTATTCTTCTGGAGGCGAAGGTGATGATGGCGGAAATATGAACATTTCATCGGAGGACCAACTAGATGGTGGCAGCGGTGACAACACTGGCGATGAGGATCAGACCCGCCACCACGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

195

Amino Acids

20.86

Weight (kDa)

5.26

Isoelectric Point (pI)

55.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endosulfine PF04667 83 - 155 1.6e-25 cAMP-regulated phosphoprotein/endosulfine conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 207
AciI CCGC 6 cut(s) 398, 412, 434, 495, 540, 571
AclWI GGATC 4 cut(s) 70, 83, 186, 570
AcuI CTGAAG 2 cut(s) 33, 234
AfaI GTAC 1 cut(s) 127
AfiI CCNNNNNNNGG 1 cut(s) 262
AgsI TTSAA 3 cut(s) 222, 404, 453
AluBI AGCT 3 cut(s) 218, 421, 464
AluI AGCT 3 cut(s) 218, 421, 464
AlwI GGATC 4 cut(s) 70, 83, 186, 570
AoxI GGCC 1 cut(s) 395
ApeKI GCWGC 4 cut(s) 269, 421, 424, 537
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 4 cut(s) 148, 328, 395, 520
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 4 cut(s) 197, 203, 497, 554
AvaII GGWCC 3 cut(s) 148, 328, 520
BaeGI GKGCMC 1 cut(s) 367
BamHI GGATCC 1 cut(s) 75
BauI CACGAG 1 cut(s) 578
BbsI GAAGAC 1 cut(s) 314
BbvI GCAGC 4 cut(s) 281, 408, 436, 549
BccI CCATC 3 cut(s) 182, 485, 524
BclI TGATCA 1 cut(s) 87
BcnI CCSGG 1 cut(s) 202
BfaI CTAG 3 cut(s) 21, 182, 527
BfmI CTRYAG 1 cut(s) 422
BfoI RGCGCY 1 cut(s) 460
BglI GCCNNNNNGGC 1 cut(s) 394
BisI GCNGC 5 cut(s) 270, 398, 422, 425, 538
BlpI GCTNAGC 1 cut(s) 460
BlsI GCNGC 5 cut(s) 271, 399, 423, 426, 539
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 3 cut(s) 148, 328, 520
BmgT120I GGNCC 4 cut(s) 148, 328, 395, 520
BmiI GGNNCC 1 cut(s) 77
BmrFI CCNGG 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 314
BpmI CTGGAG 1 cut(s) 495
Bpu10I CCTNAGC 1 cut(s) 108
Bpu1102I GCTNAGC 1 cut(s) 460
BpuMI CCSGG 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 561
BsaJI CCNNGG 3 cut(s) 201, 302, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 556
Bse8I GATNNNNATC 1 cut(s) 561
BseDI CCNNGG 3 cut(s) 201, 302, 324
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMII CTCAG 2 cut(s) 99, 474
BseNI ACTGG 1 cut(s) 556
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 1 cut(s) 367
BseXI GCAGC 4 cut(s) 281, 408, 436, 549
BseYI CCCAGC 1 cut(s) 255
BshFI GGCC 1 cut(s) 397
BsiSI CCGG 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 262
BsnI GGCC 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 367
Bsp143I GATC 5 cut(s) 75, 80, 87, 178, 562
Bsp1720I GCTNAGC 1 cut(s) 460
BspACI CCGC 6 cut(s) 398, 412, 434, 495, 540, 571
BspANI GGCC 1 cut(s) 397
BspCNI CTCAG 2 cut(s) 100, 473
BspLI GGNNCC 1 cut(s) 77
BspMAI CTGCAG 1 cut(s) 426
BspPI GGATC 4 cut(s) 70, 83, 186, 570
BsrI ACTGG 1 cut(s) 556
BssECI CCNNGG 3 cut(s) 201, 302, 324
BssMI GATC 5 cut(s) 75, 80, 87, 178, 562
BssSI CACGAG 1 cut(s) 578
BssT1I CCWWGG 2 cut(s) 302, 324
Bst2BI CACGAG 1 cut(s) 578
BstC8I GCNNGC 3 cut(s) 27, 101, 339
BstDEI CTNAG 2 cut(s) 108, 460
BstH2I RGCGCY 1 cut(s) 460
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 5 cut(s) 78, 83, 90, 181, 565
BstMBI GATC 5 cut(s) 75, 80, 87, 178, 562
BstMWI GCNNNNNNNGC 5 cut(s) 109, 362, 394, 433, 454
BstSCI CCNGG 1 cut(s) 200
BstSFI CTRYAG 1 cut(s) 422
BstSLI GKGCMC 1 cut(s) 367
BstV1I GCAGC 4 cut(s) 281, 408, 436, 549
BstV2I GAAGAC 1 cut(s) 314
BstX2I RGATCY 2 cut(s) 75, 178
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 2 cut(s) 75, 178
BsuRI GGCC 1 cut(s) 397
BtgZI GCGATG 1 cut(s) 570
BtsIMutI CAGTG 1 cut(s) 549
Cac8I GCNNGC 3 cut(s) 27, 101, 339
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 4 cut(s) 148, 328, 395, 520
Csp6I GTAC 1 cut(s) 126
CviAII CATG 3 cut(s) 133, 233, 332
CviJI RGCY 8 cut(s) 25, 218, 254, 388, 397, 421, 427, 464
CviKI_1 RGCY 8 cut(s) 25, 218, 254, 388, 397, 421, 427, 464
CviQI GTAC 1 cut(s) 126
DdeI CTNAG 2 cut(s) 108, 460
DpnI GATC 5 cut(s) 77, 82, 89, 180, 564
DpnII GATC 5 cut(s) 75, 80, 87, 178, 562
EciI GGCGGA 2 cut(s) 401, 510
Eco130I CCWWGG 2 cut(s) 302, 324
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 3 cut(s) 148, 328, 520
Eco57I CTGAAG 2 cut(s) 33, 234
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 2 cut(s) 302, 324
ErhI CCWWGG 2 cut(s) 302, 324
FaeI CATG 3 cut(s) 136, 236, 335
FaiI YATR 8 cut(s) 119, 134, 176, 234, 288, 296, 333, 503
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 3 cut(s) 132, 232, 331
FauI CCCGC 1 cut(s) 578
FbaI TGATCA 1 cut(s) 87
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 5 cut(s) 270, 398, 422, 425, 538
Fsp4HI GCNGC 5 cut(s) 270, 398, 422, 425, 538
FspBI CTAG 3 cut(s) 21, 182, 527
GlaI GCGC 1 cut(s) 458
GluI GCNGC 5 cut(s) 270, 398, 422, 425, 538
GsaI CCCAGC 1 cut(s) 259
GsuI CTGGAG 1 cut(s) 495
HaeII RGCGCY 1 cut(s) 460
HaeIII GGCC 1 cut(s) 397
HapII CCGG 1 cut(s) 201
HhaI GCGC 1 cut(s) 459
Hin1II CATG 3 cut(s) 136, 236, 335
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 2 cut(s) 310, 350
HpaII CCGG 1 cut(s) 201
HphI GGTGA 4 cut(s) 197, 203, 497, 554
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 5 cut(s) 52, 75, 80, 517, 567
Hpy188III TCNNGA 1 cut(s) 474
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 2 cut(s) 258, 476
HpyCH4V TGCA 3 cut(s) 69, 383, 424
HpyF10VI GCNNNNNNNGC 5 cut(s) 109, 362, 394, 433, 454
HpyF3I CTNAG 2 cut(s) 108, 460
Hsp92II CATG 3 cut(s) 136, 236, 335
HspAI GCGC 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 87
Kzo9I GATC 5 cut(s) 75, 80, 87, 178, 562
LpnPI CCDG 9 cut(s) 108, 113, 120, 214, 245, 269, 452, 459, 537
Lsp1109I GCAGC 4 cut(s) 281, 408, 436, 549
MaeI CTAG 3 cut(s) 21, 182, 527
MaeIII GTNAC 2 cut(s) 191, 542
MalI GATC 5 cut(s) 77, 82, 89, 180, 564
MboI GATC 5 cut(s) 75, 80, 87, 178, 562
MboII GAAGA 8 cut(s) 24, 27, 30, 33, 36, 39, 319, 462
MflI RGATCY 2 cut(s) 75, 178
MhlI GDGCHC 1 cut(s) 367
MluCI AATT 1 cut(s) 273
MlyI GAGTC 1 cut(s) 304
MnlI CCTC 9 cut(s) 68, 130, 133, 148, 256, 284, 470, 511, 553
MseI TTAA 2 cut(s) 144, 276
MslI CAYNNNNRTG 2 cut(s) 173, 336
MspA1I CMGCKG 1 cut(s) 540
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 202
MwoI GCNNNNNNNGC 5 cut(s) 109, 362, 394, 433, 454
NciI CCSGG 1 cut(s) 202
NdeII GATC 5 cut(s) 75, 80, 87, 178, 562
NlaIII CATG 3 cut(s) 136, 236, 335
NlaIV GGNNCC 1 cut(s) 77
NmuCI GTSAC 2 cut(s) 191, 542
PfeI GAWTC 1 cut(s) 350
PkrI GCNGC 5 cut(s) 271, 399, 423, 426, 539
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PspFI CCCAGC 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 77
PspPI GGNCC 4 cut(s) 148, 328, 395, 520
PstI CTGCAG 1 cut(s) 426
PsuI RGATCY 2 cut(s) 75, 178
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
RseI CAYNNNNRTG 2 cut(s) 173, 336
SaqAI TTAA 2 cut(s) 144, 276
SatI GCNGC 5 cut(s) 270, 398, 422, 425, 538
Sau3AI GATC 5 cut(s) 75, 80, 87, 178, 562
Sau96I GGNCC 4 cut(s) 148, 328, 395, 520
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 1 cut(s) 367
SetI ASST 8 cut(s) 144, 159, 187, 220, 382, 423, 466, 487
SfcI CTRYAG 1 cut(s) 422
SinI GGWCC 3 cut(s) 148, 328, 520
SmiMI CAYNNNNRTG 2 cut(s) 173, 336
Sse9I AATT 1 cut(s) 273
SsiI CCGC 6 cut(s) 398, 412, 434, 495, 540, 571
SspMI CTAG 3 cut(s) 21, 182, 527
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 2 cut(s) 302, 324
TasI AATT 1 cut(s) 273
TauI GCSGC 1 cut(s) 400
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 2 cut(s) 144, 276
Tru9I TTAA 2 cut(s) 144, 276
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 2 cut(s) 191, 542
TseI GCWGC 4 cut(s) 269, 421, 424, 537
Tsp45I GTSAC 2 cut(s) 191, 542
TspDTI ATGAA 3 cut(s) 240, 501, 518
TspRI CASTG 1 cut(s) 556
VpaK11BI GGWCC 3 cut(s) 148, 328, 520
XcmI CCANNNNNNNNNTGG 1 cut(s) 530
XmiI GTMKAC 1 cut(s) 207
XspI CTAG 3 cut(s) 21, 182, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.