pycom16g11680

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006540
Physical Location & Seq
Forward (+)
8110805 .. 8111872
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 603 bp
ATGGGAAGAAAAGCTGCAGCTCATTCATCATCTTCTTCAATAGATAGCAGCAACCATCCTGATCATCATCTTCCCAGCCCTACTGCTTCATCATCTTCTTCCTCTCTTTCTCGGAAAATCAATGGAAATTACTGCAATAGCCTCAGCATCACCAGAACAGATTTGAGCACTGATCTGAGACTTGGCCTCAGCATCTCTCCTTCTCATCACAGTGACTTGTCTTCCACTTCAACCCCAAGGGAGCAAGCACTGATAAACTGGCCGCCAATCAAATCTATCTTGAGGAGCACACTTGCAGGGAGAGCAGCAGACAACAGGCGCCATCCCTCCTTATTTGTGAAGGTCTACCTGGAGGGCATTCCAATTGGTAGAAAATTGAATTTGTTTGACCACGATGGTTATTCTGCTTTGATTTCATCTCTTTGCCTCATGTTCAAGACTACCATTCTATGTCCCGAGAATAATCATGTTCATTCGGACAAATATCATGTGTTAACTTATGAAGATCAGGAAGGGGACTGGATGCTGGTTGGAGATGTGCCTTGGGAGATATTCTTGACCACAGTGAAAAGGTTGAAGATCACTAGAGCAGACAGATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

201

Amino Acids

22.19

Weight (kDa)

8.71

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 78 - 196 1.4e-29 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 318
AccI GTMKAC 1 cut(s) 345
AciI CCGC 1 cut(s) 263
AcoI YGGCCR 1 cut(s) 260
AcsI RAATTY 1 cut(s) 379
AcyI GRCGYC 1 cut(s) 319
AgsI TTSAA 5 cut(s) 39, 231, 379, 436, 577
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 2 cut(s) 14, 20
AluI AGCT 2 cut(s) 14, 20
Alw21I GWGCWC 2 cut(s) 170, 290
Alw26I GTCTC 1 cut(s) 172
Ama87I CYCGRG 1 cut(s) 455
AoxI GGCC 2 cut(s) 184, 260
ApeKI GCWGC 4 cut(s) 14, 17, 48, 305
ApoI RAATTY 1 cut(s) 379
AspLEI GCGC 1 cut(s) 321
AsuHPI GGTGA 1 cut(s) 142
AvaI CYCGRG 1 cut(s) 455
BanI GGYRCC 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 2 cut(s) 170, 290
BbvCI CCTCAGC 2 cut(s) 143, 188
BbvI GCAGC 3 cut(s) 29, 60, 317
BccI CCATC 3 cut(s) 63, 330, 389
BciT130I CCWGG 1 cut(s) 350
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 1 cut(s) 585
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 1 cut(s) 322
BisI GCNGC 5 cut(s) 15, 18, 49, 263, 306
BlsI GCNGC 5 cut(s) 16, 19, 50, 264, 307
Bme1390I CCNGG 1 cut(s) 350
BmeT110I CYCGRG 1 cut(s) 455
BmiI GGNNCC 1 cut(s) 320
BmrFI CCNGG 1 cut(s) 350
BmsI GCATC 3 cut(s) 156, 201, 513
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 371
Bpu10I CCTNAGC 2 cut(s) 143, 188
BpuEI CTTGAG 1 cut(s) 301
BsaBI GATNNNNATC 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 236, 542
Bse1I ACTGG 2 cut(s) 263, 524
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 2 cut(s) 236, 542
BseGI GGATG 3 cut(s) 55, 322, 528
BseJI GATNNNNATC 1 cut(s) 66
BseMII CTCAG 3 cut(s) 157, 167, 202
BseNI ACTGG 2 cut(s) 263, 524
BseRI GAGGAG 1 cut(s) 298
BseXI GCAGC 3 cut(s) 29, 60, 317
BseYI CCCAGC 1 cut(s) 74
BshFI GGCC 2 cut(s) 186, 262
BshNI GGYRCC 1 cut(s) 318
BsiHKAI GWGCWC 2 cut(s) 170, 290
BsiHKCI CYCGRG 1 cut(s) 455
BslFI GGGAC 2 cut(s) 438, 530
BsmAI GTCTC 1 cut(s) 172
BsmFI GGGAC 2 cut(s) 438, 530
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 2 cut(s) 186, 262
BsoBI CYCGRG 1 cut(s) 455
Bsp1286I GDGCHC 2 cut(s) 170, 290
Bsp143I GATC 4 cut(s) 61, 172, 505, 579
BspACI CCGC 1 cut(s) 263
BspANI GGCC 2 cut(s) 186, 262
BspCNI CTCAG 3 cut(s) 156, 168, 201
BspLI GGNNCC 1 cut(s) 320
BspMAI CTGCAG 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 318
BsrI ACTGG 2 cut(s) 263, 524
BssECI CCNNGG 2 cut(s) 236, 542
BssMI GATC 4 cut(s) 61, 172, 505, 579
BssNI GRCGYC 1 cut(s) 319
BssT1I CCWWGG 2 cut(s) 236, 542
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 2 cut(s) 212, 565
BstACI GRCGYC 1 cut(s) 319
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 3 cut(s) 143, 176, 188
BstF5I GGATG 3 cut(s) 55, 322, 528
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 1 cut(s) 321
BstKTI GATC 4 cut(s) 64, 175, 508, 582
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 4 cut(s) 61, 172, 505, 579
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstNI CCWGG 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 348
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 3 cut(s) 29, 60, 317
BstV2I GAAGAC 1 cut(s) 213
BsuRI GGCC 2 cut(s) 186, 262
BtsCI GGATG 3 cut(s) 55, 322, 528
BtsIMutI CAGTG 4 cut(s) 168, 217, 248, 570
Cac8I GCNNGC 1 cut(s) 246
CfoI GCGC 1 cut(s) 321
CviAII CATG 3 cut(s) 430, 467, 488
CviJI RGCY 6 cut(s) 14, 20, 78, 141, 186, 262
CviKI_1 RGCY 6 cut(s) 14, 20, 78, 141, 186, 262
DdeI CTNAG 3 cut(s) 143, 176, 188
DinI GGCGCC 1 cut(s) 320
DpnI GATC 4 cut(s) 63, 174, 507, 581
DpnII GATC 4 cut(s) 61, 172, 505, 579
EaeI YGGCCR 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 236, 542
Eco88I CYCGRG 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 348
EcoT14I CCWWGG 2 cut(s) 236, 542
EgeI GGCGCC 1 cut(s) 320
EheI GGCGCC 1 cut(s) 320
ErhI CCWWGG 2 cut(s) 236, 542
FaeI CATG 3 cut(s) 433, 470, 491
FaiI YATR 5 cut(s) 431, 451, 468, 489, 501
FaqI GGGAC 2 cut(s) 438, 530
FatI CATG 3 cut(s) 429, 466, 487
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 5 cut(s) 15, 18, 49, 263, 306
FokI GGATG 3 cut(s) 42, 309, 535
Fsp4HI GCNGC 5 cut(s) 15, 18, 49, 263, 306
FspBI CTAG 1 cut(s) 585
GlaI GCGC 1 cut(s) 320
GluI GCNGC 5 cut(s) 15, 18, 49, 263, 306
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 371
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 2 cut(s) 186, 262
HhaI GCGC 1 cut(s) 321
Hin1I GRCGYC 1 cut(s) 319
Hin1II CATG 3 cut(s) 433, 470, 491
Hin6I GCGC 1 cut(s) 319
HinP1I GCGC 1 cut(s) 319
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HpaI GTTAAC 1 cut(s) 495
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 2 cut(s) 346, 495
Hpy188I TCNGA 3 cut(s) 114, 177, 478
Hpy188III TCNNGA 6 cut(s) 59, 280, 436, 455, 509, 556
Hpy8I GTNNAC 2 cut(s) 346, 495
HpyAV CCTTC 3 cut(s) 210, 334, 506
HpyCH4III ACNGT 2 cut(s) 212, 565
HpyCH4V TGCA 3 cut(s) 17, 135, 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 3 cut(s) 143, 176, 188
Hsp92I GRCGYC 1 cut(s) 319
Hsp92II CATG 3 cut(s) 433, 470, 491
HspAI GCGC 1 cut(s) 319
KasI GGCGCC 1 cut(s) 318
Ksp22I TGATCA 1 cut(s) 61
KspAI GTTAAC 1 cut(s) 495
Kzo9I GATC 4 cut(s) 61, 172, 505, 579
LmnI GCTCC 2 cut(s) 241, 285
Lsp1109I GCAGC 3 cut(s) 29, 60, 317
LweI GCATC 3 cut(s) 156, 201, 513
MaeI CTAG 1 cut(s) 585
MaeIII GTNAC 1 cut(s) 212
MalI GATC 4 cut(s) 63, 174, 507, 581
MboI GATC 4 cut(s) 61, 172, 505, 579
MboII GAAGA 9 cut(s) 18, 24, 27, 62, 87, 90, 213, 515, 589
MfeI CAATTG 1 cut(s) 363
MhlI GDGCHC 2 cut(s) 170, 290
MluCI AATT 4 cut(s) 127, 363, 374, 379
Mly113I GGCGCC 1 cut(s) 319
MmeI TCCRAC 1 cut(s) 511
MnlI CCTC 7 cut(s) 112, 152, 197, 276, 337, 346, 437
MseI TTAA 1 cut(s) 494
MslI CAYNNNNRTG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 350
MunI CAATTG 1 cut(s) 363
Mva1269I GAATGC 1 cut(s) 357
MvaI CCWGG 1 cut(s) 350
MwoI GCNNNNNNNGC 1 cut(s) 302
NarI GGCGCC 1 cut(s) 319
NdeII GATC 4 cut(s) 61, 172, 505, 579
NlaIII CATG 3 cut(s) 433, 470, 491
NlaIV GGNNCC 1 cut(s) 320
NmuCI GTSAC 1 cut(s) 212
PctI GAATGC 1 cut(s) 357
PkrI GCNGC 5 cut(s) 16, 19, 50, 264, 307
PluTI GGCGCC 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 348
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 320
PstI CTGCAG 1 cut(s) 19
RseI CAYNNNNRTG 1 cut(s) 210
SaqAI TTAA 1 cut(s) 494
SatI GCNGC 5 cut(s) 15, 18, 49, 263, 306
Sau3AI GATC 4 cut(s) 61, 172, 505, 579
ScrFI CCNGG 1 cut(s) 350
SduI GDGCHC 2 cut(s) 170, 290
SetI ASST 5 cut(s) 16, 22, 345, 351, 575
SfaNI GCATC 3 cut(s) 156, 201, 513
SfcI CTRYAG 1 cut(s) 15
SfoI GGCGCC 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 4 cut(s) 127, 363, 374, 379
SsiI CCGC 1 cut(s) 263
SspDI GGCGCC 1 cut(s) 318
SspMI CTAG 1 cut(s) 585
StyD4I CCNGG 1 cut(s) 348
StyI CCWWGG 2 cut(s) 236, 542
TaaI ACNGT 2 cut(s) 212, 565
TasI AATT 4 cut(s) 127, 363, 374, 379
TauI GCSGC 1 cut(s) 265
Tru1I TTAA 1 cut(s) 494
Tru9I TTAA 1 cut(s) 494
TscAI CASTG 4 cut(s) 175, 217, 255, 570
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 4 cut(s) 14, 17, 48, 305
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 5 cut(s) 15, 78, 405, 461, 516
TspRI CASTG 4 cut(s) 175, 217, 255, 570
XapI RAATTY 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.