pycom16g15020

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003054
Physical Location & Seq
Forward (+)
11006815 .. 11007414
600 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGGGGGGTTCTTCCTCTAAATCCAAGACAACCACCACCACAAACAAAAGCGCCAAAGACAGAACATTCACAGGCGCAGGCAACCTCATCAAGCTCCTCCCGACCGGCACGGTCTTCTTGTTCCAGTTCCTGAACCCTGTCTTAACCAACAACGGCAACTGCACGGCCCTCAACAAATACCTAAGCGCCATTTTCATCTTCCTTTGCGGCTTCTCTTGCTGCTTTGCTTCCTTCACAGACAGTTACACTGGGAGTGATGGAAAAACACACTATGGGGTGGCAACATGCAAGGGTCTTTGGCCATCAACAAACTCCAATTCTGTAGACTTGTCTGCTTATAAGCTTAGGATTGGGGACTTTGTGCATGCCTTCTTTTCCGTGATTGTGTTTGCAGTTCTATCACTTTTGGATACCAACACTGTGAGGTGCTATTATCCAGGGTTTGAGTCCACTGAGAAGACTTTGCTGCTGGTGTTGCCTCCGATTATTGGTACAATTGCCGGTACTGTTTTCGTTGTGTTCCCTAACAACCGCCATGGAATCGGGTACCCCGCGTCAAGTTCTGATTCATCACAAGACTCAGAGTCGGCGGCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

200

Amino Acids

21.23

Weight (kDa)

8.52

Isoelectric Point (pI)

28.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 25 - 183 1.1e-64 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
Acc65I GGTACC 1 cut(s) 546
AccB1I GGYRCC 1 cut(s) 546
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 554
AciI CCGC 4 cut(s) 207, 532, 552, 590
AcoI YGGCCR 1 cut(s) 299
AfaI GTAC 3 cut(s) 493, 505, 548
AfiI CCNNNNNNNGG 1 cut(s) 488
AhdI GACNNNNNGTC 1 cut(s) 583
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 2 cut(s) 94, 343
AluI AGCT 2 cut(s) 94, 343
AlwNI CAGNNNCTG 1 cut(s) 130
AoxI GGCC 2 cut(s) 165, 299
ApeKI GCWGC 2 cut(s) 219, 466
Asp718I GGTACC 1 cut(s) 546
AspLEI GCGC 3 cut(s) 53, 77, 188
AspS9I GGNCC 1 cut(s) 166
BalI TGGCCA 1 cut(s) 301
BanI GGYRCC 1 cut(s) 546
BbsI GAAGAC 2 cut(s) 106, 464
BbvI GCAGC 2 cut(s) 206, 453
BccI CCATC 2 cut(s) 251, 310
BceAI ACGGC 2 cut(s) 169, 180
BciT130I CCWGG 1 cut(s) 438
BciVI GTATCC 1 cut(s) 403
BfmI CTRYAG 1 cut(s) 321
BfoI RGCGCY 2 cut(s) 54, 189
BfuI GTATCC 1 cut(s) 403
BisI GCNGC 4 cut(s) 208, 220, 467, 591
BlsI GCNGC 4 cut(s) 209, 221, 468, 592
Bme1390I CCNGG 1 cut(s) 438
BmeRI GACNNNNNGTC 1 cut(s) 583
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 1 cut(s) 548
BmrFI CCNGG 1 cut(s) 438
BmrI ACTGGG 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 258
BpiI GAAGAC 2 cut(s) 106, 464
Bpu10I CCTNAGC 2 cut(s) 182, 344
BsaJI CCNNGG 2 cut(s) 437, 535
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse118I RCCGGY 2 cut(s) 104, 500
Bse1I ACTGG 2 cut(s) 124, 253
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 437, 535
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMII CTCAG 2 cut(s) 444, 594
BseNI ACTGG 2 cut(s) 124, 253
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 2 cut(s) 206, 453
BsgI GTGCAG 1 cut(s) 145
Bsh1236I CGCG 1 cut(s) 554
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 2 cut(s) 167, 301
BshNI GGYRCC 1 cut(s) 546
BsiEI CGRYCG 1 cut(s) 105
BsiSI CCGG 2 cut(s) 105, 501
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 1 cut(s) 488
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 2 cut(s) 167, 301
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 4 cut(s) 207, 532, 552, 590
BspANI GGCC 2 cut(s) 167, 301
BspCNI CTCAG 2 cut(s) 445, 593
BspFNI CGCG 1 cut(s) 554
BspLI GGNNCC 1 cut(s) 548
BspT107I GGYRCC 1 cut(s) 546
BsrFI RCCGGY 2 cut(s) 104, 500
BsrI ACTGG 2 cut(s) 124, 253
BssAI RCCGGY 2 cut(s) 104, 500
BssECI CCNNGG 2 cut(s) 437, 535
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 4 cut(s) 112, 242, 421, 508
BstC8I GCNNGC 2 cut(s) 79, 366
BstDEI CTNAG 4 cut(s) 182, 344, 453, 580
BstDSI CCRYGG 1 cut(s) 535
BstFNI CGCG 1 cut(s) 554
BstH2I RGCGCY 2 cut(s) 54, 189
BstHHI GCGC 3 cut(s) 53, 77, 188
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 2 cut(s) 216, 475
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 2 cut(s) 288, 368
BstSCI CCNGG 1 cut(s) 436
BstSFI CTRYAG 1 cut(s) 321
BstUI CGCG 1 cut(s) 554
BstV1I GCAGC 2 cut(s) 206, 453
BstV2I GAAGAC 2 cut(s) 106, 464
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 2 cut(s) 167, 301
BtgI CCRYGG 1 cut(s) 535
BtsIMutI CAGTG 3 cut(s) 246, 417, 450
Cac8I GCNNGC 2 cut(s) 79, 366
CaiI CAGNNNCTG 1 cut(s) 130
CfoI GCGC 3 cut(s) 53, 77, 188
Cfr10I RCCGGY 2 cut(s) 104, 500
Cfr13I GGNCC 1 cut(s) 166
CseI GACGC 1 cut(s) 543
Csp6I GTAC 3 cut(s) 492, 504, 547
CviAII CATG 3 cut(s) 285, 365, 536
CviJI RGCY 6 cut(s) 94, 167, 210, 301, 343, 593
CviKI_1 RGCY 6 cut(s) 94, 167, 210, 301, 343, 593
CviQI GTAC 3 cut(s) 492, 504, 547
DdeI CTNAG 4 cut(s) 182, 344, 453, 580
DriI GACNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 299
Eam1105I GACNNNNNGTC 1 cut(s) 583
Eco130I CCWWGG 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 535
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 3 cut(s) 288, 368, 539
FaiI YATR 5 cut(s) 273, 286, 339, 366, 537
FaqI GGGAC 1 cut(s) 368
FatI CATG 3 cut(s) 284, 364, 535
FauI CCCGC 1 cut(s) 559
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 4 cut(s) 208, 220, 467, 591
Fsp4HI GCNGC 4 cut(s) 208, 220, 467, 591
GlaI GCGC 3 cut(s) 52, 76, 187
GluI GCNGC 4 cut(s) 208, 220, 467, 591
HaeII RGCGCY 2 cut(s) 54, 189
HaeIII GGCC 2 cut(s) 167, 301
HapII CCGG 2 cut(s) 105, 501
HgaI GACGC 1 cut(s) 543
HhaI GCGC 3 cut(s) 53, 77, 188
Hin1II CATG 3 cut(s) 288, 368, 539
Hin6I GCGC 3 cut(s) 51, 75, 186
HinP1I GCGC 3 cut(s) 51, 75, 186
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 5 cut(s) 446, 540, 566, 578, 584
HpaII CCGG 2 cut(s) 105, 501
Hpy166II GTNNAC 2 cut(s) 325, 450
Hpy188I TCNGA 3 cut(s) 483, 565, 583
Hpy188III TCNNGA 2 cut(s) 100, 130
Hpy8I GTNNAC 2 cut(s) 325, 450
HpyAV CCTTC 2 cut(s) 241, 379
HpyCH4III ACNGT 4 cut(s) 112, 242, 421, 508
HpyCH4V TGCA 4 cut(s) 162, 288, 364, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 216, 475
HpyF3I CTNAG 4 cut(s) 182, 344, 453, 580
Hsp92II CATG 3 cut(s) 288, 368, 539
HspAI GCGC 3 cut(s) 51, 75, 186
KpnI GGTACC 1 cut(s) 550
LmnI GCTCC 1 cut(s) 99
Lsp1109I GCAGC 2 cut(s) 206, 453
MaeIII GTNAC 1 cut(s) 242
MboII GAAGA 4 cut(s) 3, 106, 190, 469
MfeI CAATTG 1 cut(s) 495
MlsI TGGCCA 1 cut(s) 301
MluCI AATT 2 cut(s) 316, 495
MluNI TGGCCA 1 cut(s) 301
MlyI GAGTC 3 cut(s) 455, 572, 593
MnlI CCTC 6 cut(s) 25, 95, 107, 179, 417, 489
Mox20I TGGCCA 1 cut(s) 301
MscI TGGCCA 1 cut(s) 301
MseI TTAA 1 cut(s) 143
Msp20I TGGCCA 1 cut(s) 301
MspI CCGG 2 cut(s) 105, 501
MspR9I CCNGG 1 cut(s) 438
MunI CAATTG 1 cut(s) 495
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 554
MwoI GCNNNNNNNGC 2 cut(s) 216, 475
NcoI CCATGG 1 cut(s) 535
NlaIII CATG 3 cut(s) 288, 368, 539
NlaIV GGNNCC 1 cut(s) 548
NspI RCATGY 2 cut(s) 288, 368
PaeI GCATGC 1 cut(s) 368
PfeI GAWTC 2 cut(s) 540, 566
PkrI GCNGC 4 cut(s) 209, 221, 468, 592
PleI GAGTC 3 cut(s) 454, 572, 592
PpsI GAGTC 3 cut(s) 454, 572, 592
PsiI TTATAA 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 1 cut(s) 548
PspPI GGNCC 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 130
RsaI GTAC 3 cut(s) 493, 505, 548
RsaNI GTAC 3 cut(s) 492, 504, 547
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 4 cut(s) 208, 220, 467, 591
Sau96I GGNCC 1 cut(s) 166
SchI GAGTC 3 cut(s) 455, 572, 593
ScrFI CCNGG 1 cut(s) 438
SetI ASST 5 cut(s) 87, 96, 183, 345, 428
SfcI CTRYAG 1 cut(s) 321
SphI GCATGC 1 cut(s) 368
Sse9I AATT 2 cut(s) 316, 495
SsiI CCGC 4 cut(s) 207, 532, 552, 590
StyD4I CCNGG 1 cut(s) 436
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 4 cut(s) 112, 242, 421, 508
TasI AATT 2 cut(s) 316, 495
TauI GCSGC 2 cut(s) 210, 593
TfiI GAWTC 2 cut(s) 540, 566
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TscAI CASTG 3 cut(s) 253, 424, 457
TseI GCWGC 2 cut(s) 219, 466
TspDTI ATGAA 2 cut(s) 184, 558
TspGWI ACGGA 1 cut(s) 367
TspRI CASTG 3 cut(s) 253, 424, 457
XceI RCATGY 2 cut(s) 288, 368
XmiI GTMKAC 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.