pycom16g16320

Belongs to the imidazoleglycerol-phosphate dehydratase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007204
Physical Location & Seq
Forward (+)
12273498 .. 12276171
2674 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 690 bp
ATGGACTCTCAGAGAGCAGCTCCGCCGCGCGCCTCCCTGGCCGAAAACAACGGCTCCACTCTCACCGATACCCCAACCCCAATTAACTCAGGGGCTCGAATTGGGGAGGTCAAGAGGGTGACTAAAGAGACCAATGTCTCGGTCAAGATCGACCTGGACGGCACTGGAATCGCTGAATCCAGTACTGGGATTCCGTTTCTTGATCATATGTTGGATCAACTTGCTTCGCACGGGTTGCTGGATGTGCATGTAAAGGCTACCGGTGACATCCACATTGATGATCATCACACAAATGAAGATGTTGCTCTTGCTATAGGAACGGCTTTGCTTCAAGCACTTGGCGATAGAAAAGGAATCAACAGGTTTGGTGACTTCTCTGCTCCACTTGATGAAGCACTCATACATGTCTCACTGGATTTATCTGGCCGGCCACATTTGAGTTATGATCTCCAGATACCCACCGAGAGGGTTGGCACATATGACACTCAGCTGGTGGAACATTTTTTCCAGTCTTTGGTGAATACTTCTGGTATGACACTCCACATCCGGCAGCTTGCTGGAAGAAATTCTCACCATATTATTGAGGCAACCTTCAAAGCATTTGCAAGGGCTCTCCGACAAGCAACAGAATCTGACCCACGTCGCCTGGGGACCGTGCCAAGTTCGAAAGGTGTCTTGTCACGCTCTTGA

Protein Analysis

230

Amino Acids

24.74

Weight (kDa)

6.03

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IGPD PF00475 62 - 205 1.1e-64 Imidazoleglycerol-phosphate dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 514
AccII CGCG 2 cut(s) 28, 30
AciI CCGC 2 cut(s) 23, 26
AclWI GGATC 1 cut(s) 222
AcoI YGGCCR 3 cut(s) 39, 424, 428
AcsI RAATTY 1 cut(s) 565
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 3 cut(s) 186, 465, 514
AflIII ACRYGT 1 cut(s) 403
AgeI ACCGGT 1 cut(s) 260
AgsI TTSAA 2 cut(s) 332, 595
AjiI CACGTC 1 cut(s) 641
AjnI CCWGG 3 cut(s) 36, 153, 645
AloI GAACNNNNNNTCC 2 cut(s) 489, 521
AluBI AGCT 3 cut(s) 20, 490, 553
AluI AGCT 3 cut(s) 20, 490, 553
Alw26I GTCTC 3 cut(s) 122, 142, 412
AlwI GGATC 1 cut(s) 222
AlwNI CAGNNNCTG 1 cut(s) 632
AoxI GGCC 3 cut(s) 39, 424, 428
ApeKI GCWGC 2 cut(s) 17, 550
ApoI RAATTY 1 cut(s) 565
AsiGI ACCGGT 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 565
AspLEI GCGC 2 cut(s) 30, 32
AspS9I GGNCC 1 cut(s) 651
AsuHPI GGTGA 6 cut(s) 55, 130, 275, 380, 529, 563
AsuII TTCGAA 1 cut(s) 665
AvaII GGWCC 1 cut(s) 651
BanII GRGCYC 2 cut(s) 97, 613
BarI GAAGNNNNNNTAC 2 cut(s) 384, 416
BbvI GCAGC 2 cut(s) 29, 562
BceAI ACGGC 3 cut(s) 67, 175, 336
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 3 cut(s) 38, 155, 647
BclI TGATCA 2 cut(s) 202, 280
BcoDI GTCTC 3 cut(s) 122, 142, 412
BfmI CTRYAG 1 cut(s) 312
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 3 cut(s) 18, 26, 551
BlsI GCNGC 3 cut(s) 19, 27, 552
BmcAI AGTACT 1 cut(s) 184
Bme1390I CCNGG 3 cut(s) 38, 155, 647
Bme18I GGWCC 1 cut(s) 651
BmgBI CACGTC 1 cut(s) 641
BmgT120I GGNCC 1 cut(s) 651
BmiI GGNNCC 2 cut(s) 55, 652
BmrFI CCNGG 3 cut(s) 38, 155, 647
BmrI ACTGGG 1 cut(s) 195
BmuI ACTGGG 1 cut(s) 195
BoxI GACNNNNGTC 2 cut(s) 134, 639
BplI GAGNNNNNCTC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 434
Bpu14I TTCGAA 1 cut(s) 665
BsaBI GATNNNNATC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 2 cut(s) 36, 646
BsaWI WCCGGW 1 cut(s) 260
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 3 cut(s) 186, 465, 514
Bse118I RCCGGY 2 cut(s) 260, 426
Bse1I ACTGG 5 cut(s) 169, 180, 190, 417, 508
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 3 cut(s) 38, 155, 647
BseDI CCNNGG 2 cut(s) 36, 646
BseGI GGATG 3 cut(s) 247, 267, 543
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 3 cut(s) 186, 465, 514
BseMII CTCAG 3 cut(s) 23, 102, 500
BseNI ACTGG 5 cut(s) 169, 180, 190, 417, 508
BsePI GCGCGC 1 cut(s) 28
BseXI GCAGC 2 cut(s) 29, 562
Bsh1236I CGCG 2 cut(s) 28, 30
BshFI GGCC 3 cut(s) 41, 426, 430
BshTI ACCGGT 1 cut(s) 260
BsiSI CCGG 3 cut(s) 261, 427, 547
BslFI GGGAC 1 cut(s) 664
BslI CCNNNNNNNGG 3 cut(s) 186, 465, 514
BsmAI GTCTC 3 cut(s) 122, 142, 412
BsmFI GGGAC 1 cut(s) 664
BsnI GGCC 3 cut(s) 41, 426, 430
Bso31I GGTCTC 1 cut(s) 122
Bsp119I TTCGAA 1 cut(s) 665
Bsp1286I GDGCHC 2 cut(s) 97, 613
Bsp143I GATC 5 cut(s) 147, 202, 214, 280, 445
BspACI CCGC 2 cut(s) 23, 26
BspANI GGCC 3 cut(s) 41, 426, 430
BspCNI CTCAG 3 cut(s) 22, 101, 499
BspFNI CGCG 2 cut(s) 28, 30
BspLI GGNNCC 2 cut(s) 55, 652
BspPI GGATC 1 cut(s) 222
BspT104I TTCGAA 1 cut(s) 665
BspTNI GGTCTC 1 cut(s) 122
BsrFI RCCGGY 2 cut(s) 260, 426
BsrI ACTGG 5 cut(s) 169, 180, 190, 417, 508
BssAI RCCGGY 2 cut(s) 260, 426
BssECI CCNNGG 2 cut(s) 36, 646
BssHII GCGCGC 1 cut(s) 28
BssMI GATC 5 cut(s) 147, 202, 214, 280, 445
Bst2UI CCWGG 3 cut(s) 38, 155, 647
Bst4CI ACNGT 1 cut(s) 655
BstAPI GCANNNNNTGC 1 cut(s) 235
BstBI TTCGAA 1 cut(s) 665
BstC8I GCNNGC 3 cut(s) 30, 428, 555
BstDEI CTNAG 3 cut(s) 9, 88, 486
BstF5I GGATG 3 cut(s) 247, 267, 543
BstFNI CGCG 2 cut(s) 28, 30
BstHHI GCGC 2 cut(s) 30, 32
BstKTI GATC 5 cut(s) 150, 205, 217, 283, 448
BstMAI GTCTC 3 cut(s) 122, 142, 412
BstMBI GATC 5 cut(s) 147, 202, 214, 280, 445
BstMWI GCNNNNNNNGC 3 cut(s) 38, 235, 244
BstNI CCWGG 3 cut(s) 38, 155, 647
BstNSI RCATGY 2 cut(s) 251, 407
BstPAI GACNNNNGTC 2 cut(s) 134, 639
BstSCI CCNGG 3 cut(s) 36, 153, 645
BstSFI CTRYAG 1 cut(s) 312
BstUI CGCG 2 cut(s) 28, 30
BstV1I GCAGC 2 cut(s) 29, 562
BsuRI GGCC 3 cut(s) 41, 426, 430
BtrI CACGTC 1 cut(s) 641
BtsCI GGATG 3 cut(s) 247, 267, 543
BtsIMutI CAGTG 2 cut(s) 162, 410
Cac8I GCNNGC 3 cut(s) 30, 428, 555
CaiI CAGNNNCTG 1 cut(s) 632
CfoI GCGC 2 cut(s) 30, 32
Cfr10I RCCGGY 2 cut(s) 260, 426
Cfr13I GGNCC 1 cut(s) 651
Csp6I GTAC 1 cut(s) 183
CspAI ACCGGT 1 cut(s) 260
CviAII CATG 2 cut(s) 248, 404
CviQI GTAC 1 cut(s) 183
DdeI CTNAG 3 cut(s) 9, 88, 486
DpnI GATC 5 cut(s) 149, 204, 216, 282, 447
DpnII GATC 5 cut(s) 147, 202, 214, 280, 445
EaeI YGGCCR 3 cut(s) 39, 424, 428
EciI GGCGGA 1 cut(s) 12
Eco24I GRGCYC 2 cut(s) 97, 613
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 651
EcoRII CCWGG 3 cut(s) 36, 153, 645
EcoT38I GRGCYC 2 cut(s) 97, 613
FaeI CATG 2 cut(s) 251, 407
FaqI GGGAC 1 cut(s) 664
FatI CATG 2 cut(s) 247, 403
FauNDI CATATG 2 cut(s) 207, 478
FbaI TGATCA 2 cut(s) 202, 280
Fnu4HI GCNGC 3 cut(s) 18, 26, 551
FokI GGATG 3 cut(s) 254, 254, 530
FriOI GRGCYC 2 cut(s) 97, 613
FseI GGCCGGCC 1 cut(s) 430
Fsp4HI GCNGC 3 cut(s) 18, 26, 551
GlaI GCGC 2 cut(s) 29, 31
GluI GCNGC 3 cut(s) 18, 26, 551
GsuI CTGGAG 1 cut(s) 434
HaeIII GGCC 3 cut(s) 41, 426, 430
HapII CCGG 3 cut(s) 261, 427, 547
HhaI GCGC 2 cut(s) 30, 32
Hin1II CATG 2 cut(s) 251, 407
Hin6I GCGC 2 cut(s) 28, 30
HinP1I GCGC 2 cut(s) 28, 30
HinfI GANTC 6 cut(s) 5, 168, 176, 190, 354, 629
HpaII CCGG 3 cut(s) 261, 427, 547
HphI GGTGA 6 cut(s) 55, 130, 275, 380, 529, 563
Hpy188I TCNGA 3 cut(s) 12, 617, 634
Hpy188III TCNNGA 5 cut(s) 112, 145, 200, 451, 687
Hpy99I CGWCG 1 cut(s) 645
HpyAV CCTTC 1 cut(s) 601
HpyCH4III ACNGT 1 cut(s) 655
HpyCH4IV ACGT 1 cut(s) 640
HpyCH4V TGCA 2 cut(s) 247, 605
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 235, 244
HpyF3I CTNAG 3 cut(s) 9, 88, 486
HpySE526I ACGT 1 cut(s) 640
Hsp92II CATG 2 cut(s) 251, 407
HspAI GCGC 2 cut(s) 28, 30
KroI GCCGGC 1 cut(s) 426
KroNI GCCGGC 1 cut(s) 428
Ksp22I TGATCA 2 cut(s) 202, 280
Kzo9I GATC 5 cut(s) 147, 202, 214, 280, 445
LmnI GCTCC 3 cut(s) 25, 59, 385
Lsp1109I GCAGC 2 cut(s) 29, 562
MaeII ACGT 1 cut(s) 640
MaeIII GTNAC 4 cut(s) 118, 263, 368, 678
MalI GATC 5 cut(s) 149, 204, 216, 282, 447
MboI GATC 5 cut(s) 147, 202, 214, 280, 445
MboII GAAGA 2 cut(s) 308, 573
MhlI GDGCHC 2 cut(s) 97, 613
MluCI AATT 3 cut(s) 81, 99, 565
MmeI TCCRAC 2 cut(s) 192, 640
MnlI CCTC 5 cut(s) 43, 100, 108, 459, 577
MroNI GCCGGC 1 cut(s) 426
MroXI GAANNNNTTC 1 cut(s) 565
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 2 cut(s) 276, 291
MspA1I CMGCKG 1 cut(s) 490
MspI CCGG 3 cut(s) 261, 427, 547
MspR9I CCNGG 3 cut(s) 38, 155, 647
MvaI CCWGG 3 cut(s) 38, 155, 647
MvnI CGCG 2 cut(s) 28, 30
MwoI GCNNNNNNNGC 3 cut(s) 38, 235, 244
NaeI GCCGGC 1 cut(s) 428
NdeI CATATG 2 cut(s) 207, 478
NdeII GATC 5 cut(s) 147, 202, 214, 280, 445
NgoMIV GCCGGC 1 cut(s) 426
NlaIII CATG 2 cut(s) 251, 407
NlaIV GGNNCC 2 cut(s) 55, 652
NmuCI GTSAC 4 cut(s) 118, 263, 368, 678
NspI RCATGY 2 cut(s) 251, 407
NspV TTCGAA 1 cut(s) 665
PauI GCGCGC 1 cut(s) 28
PciI ACATGT 1 cut(s) 403
PdiI GCCGGC 1 cut(s) 428
PdmI GAANNNNTTC 1 cut(s) 565
PfeI GAWTC 5 cut(s) 168, 176, 190, 354, 629
PflMI CCANNNNNTGG 1 cut(s) 514
PinAI ACCGGT 1 cut(s) 260
PkrI GCNGC 3 cut(s) 19, 27, 552
PscI ACATGT 1 cut(s) 403
PshAI GACNNNNGTC 2 cut(s) 134, 639
Psp6I CCWGG 3 cut(s) 36, 153, 645
PspGI CCWGG 3 cut(s) 36, 153, 645
PspN4I GGNNCC 2 cut(s) 55, 652
PspPI GGNCC 1 cut(s) 651
PstNI CAGNNNCTG 1 cut(s) 632
PteI GCGCGC 1 cut(s) 28
PvuII CAGCTG 1 cut(s) 490
RigI GGCCGGCC 1 cut(s) 430
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
RseI CAYNNNNRTG 2 cut(s) 276, 291
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 3 cut(s) 18, 26, 551
Sau3AI GATC 5 cut(s) 147, 202, 214, 280, 445
Sau96I GGNCC 1 cut(s) 651
ScaI AGTACT 1 cut(s) 184
ScrFI CCNGG 3 cut(s) 38, 155, 647
SduI GDGCHC 2 cut(s) 97, 613
SetI ASST 9 cut(s) 22, 111, 156, 365, 492, 555, 593, 643, 673
SfcI CTRYAG 1 cut(s) 312
SfuI TTCGAA 1 cut(s) 665
SinI GGWCC 1 cut(s) 651
SmiMI CAYNNNNRTG 2 cut(s) 276, 291
Sse9I AATT 3 cut(s) 81, 99, 565
SsiI CCGC 2 cut(s) 23, 26
StyD4I CCNGG 3 cut(s) 36, 153, 645
TaaI ACNGT 1 cut(s) 655
TaiI ACGT 1 cut(s) 643
TaqI TCGA 3 cut(s) 97, 150, 665
TaqII GACCGA 1 cut(s) 130
TasI AATT 3 cut(s) 81, 99, 565
TatI WGTACW 1 cut(s) 182
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 5 cut(s) 168, 176, 190, 354, 629
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 2 cut(s) 169, 417
TseFI GTSAC 4 cut(s) 118, 263, 368, 678
TseI GCWGC 2 cut(s) 17, 550
Tsp45I GTSAC 4 cut(s) 118, 263, 368, 678
TspDTI ATGAA 2 cut(s) 309, 405
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 2 cut(s) 169, 417
Van91I CCANNNNNTGG 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 651
XapI RAATTY 1 cut(s) 565
XceI RCATGY 2 cut(s) 251, 407
XmnI GAANNNNTTC 1 cut(s) 565
ZrmI AGTACT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.