Rh4AG202300

Belongs to the imidazoleglycerol-phosphate dehydratase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007204
Physical Location & Seq
Reverse (-)
48752431 .. 48754879
2449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG202300.1

Sequence Viewer

Length: 825 bp
ATGGAGGTATCAGCACCACCCGGTGTTCTAACCTCACCATCCTCATTCTCATCTCCGCCGCTCAAACCCAGACTCCTCACCTTCGCCAATTCCCGGTGGGCACATCTCCCACGAAAGCACCACCACCTGAAGCTCCAACACATGGACTCTCACTCTCACGGAATCATCACTCCCTCTGCCTCTTACAATGGCTCCACTCTCACCACCGATTCCCCTTCTGGCTCTGGGAAGAGAATTGGGGAGGTTAAGAGGGAGACCAAGGAGACCAATGTCTCCGTCAAAATAAACCTTGACGGCACCGGAATTGCTGATTCCAGTACTGGGATTCCCTTTCTTGATCATATGTTGGATCAACTTGCTTCGCACGGGTTGTTGGACGTGCATGTGAGGGCAGCTGGTGACACCCACATTGATGATCATCACACAAATGAAGACGTCGCTCTTGCTATTGGAACGGCTTTGCTTCAAGCACTTGGTGATAGAAAAGGAATCAACAGGTTTGGTGACTTCTCTGCTCCTCTTGATGAAGCATGGATTCATGTCTCACTGGATTTATCTGGCCGACCACATTTGGGTTATGATCTTCAGATACCCACCGAGAGAGTTGGTACCTATGACACTCAGCTGGTGGAACATTTTTTCCAGTCTTTGGTGAATACTTCTGGTATGACACTTCATATCCGGCAGCTTGCTGGAAGGAACTCTCACCATATTATTGAGGCAACCTTCAAAGCATTTGCAAGGGCTCTTCGACAAGCACTAGAATATGACCCACGTCGCCTTGGGAGCATTCCAAGCTCGAAAGGTGTCCTGTCACGTTCTTGA

Protein Analysis

274

Amino Acids

29.9

Weight (kDa)

7.05

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IGPD PF00475 107 - 250 3.8e-63 Imidazoleglycerol-phosphate dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 438
Acc65I GGTACC 1 cut(s) 608
AccB1I GGYRCC 2 cut(s) 296, 608
AccB7I CCANNNNNTGG 2 cut(s) 142, 649
AccBSI CCGCTC 1 cut(s) 61
AciI CCGC 2 cut(s) 56, 59
AclWI GGATC 1 cut(s) 357
AcoI YGGCCR 1 cut(s) 559
AcuI CTGAAG 2 cut(s) 149, 569
AcyI GRCGYC 1 cut(s) 435
AdeI CACNNNGTG 2 cut(s) 23, 476
AfaI GTAC 2 cut(s) 319, 610
AfiI CCNNNNNNNGG 5 cut(s) 93, 142, 321, 572, 649
AgsI TTSAA 2 cut(s) 467, 730
AjiI CACGTC 2 cut(s) 379, 776
AloI GAACNNNNNNTCC 2 cut(s) 624, 656
AluBI AGCT 5 cut(s) 133, 395, 625, 688, 798
AluI AGCT 5 cut(s) 133, 395, 625, 688, 798
Alw26I GTCTC 4 cut(s) 248, 257, 277, 547
AlwI GGATC 1 cut(s) 357
AoxI GGCC 1 cut(s) 559
ApeKI GCWGC 2 cut(s) 392, 685
Asp718I GGTACC 1 cut(s) 608
AsuC2I CCSGG 2 cut(s) 21, 94
AsuHPI GGTGA 8 cut(s) 27, 70, 193, 410, 488, 515, 664, 698
BaeGI GKGCMC 1 cut(s) 103
BanI GGYRCC 2 cut(s) 296, 608
BanII GRGCYC 1 cut(s) 748
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 2 cut(s) 404, 697
BccI CCATC 1 cut(s) 46
BceAI ACGGC 2 cut(s) 310, 471
BclI TGATCA 2 cut(s) 337, 415
BcnI CCSGG 2 cut(s) 21, 94
BcoDI GTCTC 4 cut(s) 248, 257, 277, 547
BfaI CTAG 1 cut(s) 761
BisI GCNGC 3 cut(s) 59, 393, 686
BlsI GCNGC 3 cut(s) 60, 394, 687
BmcAI AGTACT 1 cut(s) 319
Bme1390I CCNGG 2 cut(s) 21, 94
BmgBI CACGTC 2 cut(s) 379, 776
BmiI GGNNCC 3 cut(s) 193, 298, 610
BmrFI CCNGG 2 cut(s) 21, 94
BmrI ACTGGG 1 cut(s) 330
BmuI ACTGGG 1 cut(s) 330
BoxI GACNNNNGTC 2 cut(s) 269, 774
BpiI GAAGAC 1 cut(s) 438
BpuMI CCSGG 2 cut(s) 21, 94
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 435
BsaI GGTCTC 2 cut(s) 248, 257
BsaJI CCNNGG 2 cut(s) 258, 781
BsaWI WCCGGW 1 cut(s) 299
BsaXI ACNNNNNCTCC 4 cut(s) 57, 87, 176, 206
Bsc4I CCNNNNNNNGG 5 cut(s) 93, 142, 321, 572, 649
Bse1I ACTGG 4 cut(s) 315, 325, 552, 643
Bse8I GATNNNNATC 1 cut(s) 417
BseDI CCNNGG 2 cut(s) 258, 781
BseGI GGATG 1 cut(s) 38
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 5 cut(s) 93, 142, 321, 572, 649
BseMII CTCAG 1 cut(s) 635
BseNI ACTGG 4 cut(s) 315, 325, 552, 643
BseRI GAGGAG 2 cut(s) 65, 507
BseSI GKGCMC 1 cut(s) 103
BseXI GCAGC 2 cut(s) 404, 697
BshFI GGCC 1 cut(s) 561
BshNI GGYRCC 2 cut(s) 296, 608
BsiSI CCGG 4 cut(s) 21, 94, 300, 682
BslI CCNNNNNNNGG 5 cut(s) 93, 142, 321, 572, 649
BsmAI GTCTC 4 cut(s) 248, 257, 277, 547
BsmI GAATGC 1 cut(s) 789
BsnI GGCC 1 cut(s) 561
Bso31I GGTCTC 2 cut(s) 248, 257
Bsp1286I GDGCHC 2 cut(s) 103, 748
Bsp143I GATC 4 cut(s) 337, 349, 415, 580
BspACI CCGC 2 cut(s) 56, 59
BspANI GGCC 1 cut(s) 561
BspCNI CTCAG 1 cut(s) 634
BspLI GGNNCC 3 cut(s) 193, 298, 610
BspPI GGATC 1 cut(s) 357
BspQI GCTCTTC 1 cut(s) 753
BspT107I GGYRCC 2 cut(s) 296, 608
BspTNI GGTCTC 2 cut(s) 248, 257
BsrBI CCGCTC 1 cut(s) 61
BsrI ACTGG 4 cut(s) 315, 325, 552, 643
BssECI CCNNGG 2 cut(s) 258, 781
BssMI GATC 4 cut(s) 337, 349, 415, 580
BssNI GRCGYC 1 cut(s) 435
BssT1I CCWWGG 2 cut(s) 258, 781
Bst6I CTCTTC 2 cut(s) 224, 753
BstACI GRCGYC 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 690
BstDEI CTNAG 1 cut(s) 621
BstF5I GGATG 1 cut(s) 38
BstKTI GATC 4 cut(s) 340, 352, 418, 583
BstMAI GTCTC 4 cut(s) 248, 257, 277, 547
BstMBI GATC 4 cut(s) 337, 349, 415, 580
BstMWI GCNNNNNNNGC 2 cut(s) 786, 795
BstNSI RCATGY 1 cut(s) 386
BstPAI GACNNNNGTC 2 cut(s) 269, 774
BstSCI CCNGG 2 cut(s) 19, 92
BstSLI GKGCMC 1 cut(s) 103
BstV1I GCAGC 2 cut(s) 404, 697
BstV2I GAAGAC 1 cut(s) 438
BsuRI GGCC 1 cut(s) 561
BtrI CACGTC 2 cut(s) 379, 776
BtsCI GGATG 1 cut(s) 38
BtsIMutI CAGTG 1 cut(s) 545
Cac8I GCNNGC 1 cut(s) 690
Csp6I GTAC 2 cut(s) 318, 609
CviAII CATG 4 cut(s) 142, 383, 531, 539
CviQI GTAC 2 cut(s) 318, 609
DdeI CTNAG 1 cut(s) 621
DpnI GATC 4 cut(s) 339, 351, 417, 582
DpnII GATC 4 cut(s) 337, 349, 415, 580
DraIII CACNNNGTG 2 cut(s) 23, 476
EaeI YGGCCR 1 cut(s) 559
Eam1104I CTCTTC 2 cut(s) 224, 753
EarI CTCTTC 2 cut(s) 224, 753
EciI GGCGGA 1 cut(s) 45
Eco130I CCWWGG 2 cut(s) 258, 781
Eco24I GRGCYC 1 cut(s) 748
Eco31I GGTCTC 2 cut(s) 248, 257
Eco57I CTGAAG 2 cut(s) 149, 569
EcoT14I CCWWGG 2 cut(s) 258, 781
EcoT38I GRGCYC 1 cut(s) 748
ErhI CCWWGG 2 cut(s) 258, 781
FaeI CATG 4 cut(s) 145, 386, 534, 542
FatI CATG 4 cut(s) 141, 382, 530, 538
FauNDI CATATG 1 cut(s) 342
FbaI TGATCA 2 cut(s) 337, 415
Fnu4HI GCNGC 3 cut(s) 59, 393, 686
FokI GGATG 1 cut(s) 25
FriOI GRGCYC 1 cut(s) 748
Fsp4HI GCNGC 3 cut(s) 59, 393, 686
FspBI CTAG 1 cut(s) 761
GluI GCNGC 3 cut(s) 59, 393, 686
HaeIII GGCC 1 cut(s) 561
HapII CCGG 4 cut(s) 21, 94, 300, 682
Hin1I GRCGYC 1 cut(s) 435
Hin1II CATG 4 cut(s) 145, 386, 534, 542
HinfI GANTC 8 cut(s) 72, 146, 162, 209, 311, 325, 489, 535
HpaII CCGG 4 cut(s) 21, 94, 300, 682
HphI GGTGA 8 cut(s) 27, 70, 193, 410, 488, 515, 664, 698
Hpy188I TCNGA 1 cut(s) 588
Hpy188III TCNNGA 3 cut(s) 335, 521, 822
Hpy99I CGWCG 2 cut(s) 440, 780
HpyAV CCTTC 4 cut(s) 91, 225, 690, 736
HpyCH4IV ACGT 4 cut(s) 378, 435, 775, 817
HpyCH4V TGCA 2 cut(s) 382, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 786, 795
HpyF3I CTNAG 1 cut(s) 621
HpySE526I ACGT 4 cut(s) 378, 435, 775, 817
Hsp92I GRCGYC 1 cut(s) 435
Hsp92II CATG 4 cut(s) 145, 386, 534, 542
KpnI GGTACC 1 cut(s) 612
Ksp22I TGATCA 2 cut(s) 337, 415
Kzo9I GATC 4 cut(s) 337, 349, 415, 580
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 4 cut(s) 138, 197, 520, 786
Lsp1109I GCAGC 2 cut(s) 404, 697
MaeI CTAG 1 cut(s) 761
MaeII ACGT 4 cut(s) 378, 435, 775, 817
MaeIII GTNAC 3 cut(s) 398, 503, 813
MalI GATC 4 cut(s) 339, 351, 417, 582
MbiI CCGCTC 1 cut(s) 61
MboI GATC 4 cut(s) 337, 349, 415, 580
MboII GAAGA 4 cut(s) 241, 443, 575, 740
MhlI GDGCHC 2 cut(s) 103, 748
MluCI AATT 3 cut(s) 88, 234, 303
MlyI GAGTC 2 cut(s) 66, 140
MmeI TCCRAC 3 cut(s) 160, 327, 354
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 2 cut(s) 411, 426
MspA1I CMGCKG 2 cut(s) 395, 625
MspI CCGG 4 cut(s) 21, 94, 300, 682
MspR9I CCNGG 2 cut(s) 21, 94
Mva1269I GAATGC 1 cut(s) 789
MwoI GCNNNNNNNGC 2 cut(s) 786, 795
NciI CCSGG 2 cut(s) 21, 94
NdeI CATATG 1 cut(s) 342
NdeII GATC 4 cut(s) 337, 349, 415, 580
NlaIII CATG 4 cut(s) 145, 386, 534, 542
NlaIV GGNNCC 3 cut(s) 193, 298, 610
NmuCI GTSAC 3 cut(s) 398, 503, 813
NspI RCATGY 1 cut(s) 386
PciSI GCTCTTC 1 cut(s) 753
PctI GAATGC 1 cut(s) 789
PfeI GAWTC 6 cut(s) 162, 209, 311, 325, 489, 535
PflMI CCANNNNNTGG 2 cut(s) 142, 649
PkrI GCNGC 3 cut(s) 60, 394, 687
PleI GAGTC 2 cut(s) 66, 140
PpsI GAGTC 2 cut(s) 66, 140
PshAI GACNNNNGTC 2 cut(s) 269, 774
PspN4I GGNNCC 3 cut(s) 193, 298, 610
PvuII CAGCTG 2 cut(s) 395, 625
RsaI GTAC 2 cut(s) 319, 610
RsaNI GTAC 2 cut(s) 318, 609
RseI CAYNNNNRTG 2 cut(s) 411, 426
SapI GCTCTTC 1 cut(s) 753
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 3 cut(s) 59, 393, 686
Sau3AI GATC 4 cut(s) 337, 349, 415, 580
ScaI AGTACT 1 cut(s) 319
SchI GAGTC 2 cut(s) 66, 140
ScrFI CCNGG 2 cut(s) 21, 94
SduI GDGCHC 2 cut(s) 103, 748
SmiMI CAYNNNNRTG 2 cut(s) 411, 426
Sse9I AATT 3 cut(s) 88, 234, 303
SsiI CCGC 2 cut(s) 56, 59
SspMI CTAG 1 cut(s) 761
StyD4I CCNGG 2 cut(s) 19, 92
StyI CCWWGG 2 cut(s) 258, 781
TaiI ACGT 4 cut(s) 381, 438, 778, 820
TaqI TCGA 2 cut(s) 751, 800
TasI AATT 3 cut(s) 88, 234, 303
TatI WGTACW 1 cut(s) 317
TauI GCSGC 1 cut(s) 61
TfiI GAWTC 6 cut(s) 162, 209, 311, 325, 489, 535
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 552
TseFI GTSAC 3 cut(s) 398, 503, 813
TseI GCWGC 2 cut(s) 392, 685
Tsp45I GTSAC 3 cut(s) 398, 503, 813
TspDTI ATGAA 4 cut(s) 444, 527, 540, 665
TspGWI ACGGA 2 cut(s) 174, 265
TspRI CASTG 1 cut(s) 552
Van91I CCANNNNNTGG 2 cut(s) 142, 649
XceI RCATGY 1 cut(s) 386
XspI CTAG 1 cut(s) 761
ZraI GACGTC 1 cut(s) 436
ZrmI AGTACT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.