Rw4G017110

Belongs to the imidazoleglycerol-phosphate dehydratase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007204
Physical Location & Seq
Reverse (-)
38623623 .. 38626048
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G017110.1

Sequence Viewer

Length: 834 bp
ATGGAGGTATCAGCACCACCCGGCGTTCTAAGCTCACCATCCTCACCTTTCTCCTTCTCATCTCCGCCGCTAAAACCCAGACTCCTCACCTTCGCCAATTCCCGGTGGGCACATCTCCCACGAAAGCACCACCACCTGAAGCTCCAGCACATGGACTCTCACTCTCACGGAATCATCACTCCCTCCGCCTCTTACAATGGCTCCACTCTCACCACCGATTCCCCTTCTGGCTCTGGGAAGAGAATTGGGGAGGTTAAGAGGGAGACCAAGGAGACCAATGTCTCCGTCAAAATAAACCTTGACGGCACCGGAATTGCTGATTCCAGTACTGGGATTCCCTTCCTTGATCATATGTTGGATCAACTTGCTTCGCACGGGTTGTTGGACGTGCATGTGAGGGCAACTGGTGACATCCACATTGATGATCATCACACAAATGAAGACGTCGCTCTTGCTATTGGAACGGCTCTGCTTCAAGCACTGGGTGATAGAAAAGGAATCAACAGGTTTGGTGACTTCTCTGCTCCTCTTGATGAAGCATGGATTCATGTCTCACTGGATTTATCTGGCCGACCACATTTGGGTTATGATCTTCAGATACCCACGGAGAGAGTTGGTACCTATGACACTCAGCTGGTGGAACATTTTTTCCAGTCTTTGGTGAATACTTCTGGTATGACACTTCATATCCGGCAGCTTGCTGGAAGGAACTCTCACCATATTATTGAGGCAACCTTCAAAGCATTTGCAAGGGCTCTTCGACAAGCACTAGAATATGACCCACGTCGCCTTGGGAGCATTCCAAGCTCGAAAGGTGTCCTGTCACGTTCTTGA

Protein Analysis

277

Amino Acids

30.26

Weight (kDa)

7.05

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IGPD PF00475 110 - 253 2.3e-63 Imidazoleglycerol-phosphate dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 447
Acc65I GGTACC 1 cut(s) 617
AccB1I GGYRCC 2 cut(s) 305, 617
AccB7I CCANNNNNTGG 2 cut(s) 151, 658
AciI CCGC 3 cut(s) 65, 68, 186
AclWI GGATC 1 cut(s) 366
AcoI YGGCCR 1 cut(s) 568
AcuI CTGAAG 2 cut(s) 158, 578
AcyI GRCGYC 1 cut(s) 444
AdeI CACNNNGTG 1 cut(s) 485
AfaI GTAC 2 cut(s) 328, 619
AfiI CCNNNNNNNGG 5 cut(s) 102, 151, 330, 581, 658
AgsI TTSAA 2 cut(s) 476, 739
AjiI CACGTC 2 cut(s) 388, 785
AloI GAACNNNNNNTCC 2 cut(s) 633, 665
AluBI AGCT 5 cut(s) 33, 142, 634, 697, 807
AluI AGCT 5 cut(s) 33, 142, 634, 697, 807
Alw26I GTCTC 4 cut(s) 257, 266, 286, 556
AlwI GGATC 1 cut(s) 366
AoxI GGCC 1 cut(s) 568
ApeKI GCWGC 1 cut(s) 694
Asp718I GGTACC 1 cut(s) 617
AsuC2I CCSGG 2 cut(s) 21, 103
AsuHPI GGTGA 9 cut(s) 27, 36, 79, 202, 419, 497, 524, 673, 707
BaeGI GKGCMC 1 cut(s) 112
BanI GGYRCC 2 cut(s) 305, 617
BanII GRGCYC 1 cut(s) 757
BbsI GAAGAC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 706
BccI CCATC 1 cut(s) 46
BceAI ACGGC 2 cut(s) 319, 480
BclI TGATCA 2 cut(s) 346, 424
BcnI CCSGG 2 cut(s) 21, 103
BcoDI GTCTC 4 cut(s) 257, 266, 286, 556
BfaI CTAG 1 cut(s) 770
BisI GCNGC 2 cut(s) 68, 695
BlsI GCNGC 2 cut(s) 69, 696
BmcAI AGTACT 1 cut(s) 328
Bme1390I CCNGG 2 cut(s) 21, 103
BmgBI CACGTC 2 cut(s) 388, 785
BmiI GGNNCC 3 cut(s) 202, 307, 619
BmrFI CCNGG 2 cut(s) 21, 103
BmrI ACTGGG 2 cut(s) 339, 491
BmuI ACTGGG 2 cut(s) 339, 491
BoxI GACNNNNGTC 2 cut(s) 278, 783
BpiI GAAGAC 1 cut(s) 447
BpmI CTGGAG 1 cut(s) 128
BpuMI CCSGG 2 cut(s) 21, 103
BsaBI GATNNNNATC 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 444
BsaI GGTCTC 2 cut(s) 257, 266
BsaJI CCNNGG 3 cut(s) 267, 603, 790
BsaWI WCCGGW 1 cut(s) 308
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 185, 215
Bsc4I CCNNNNNNNGG 5 cut(s) 102, 151, 330, 581, 658
Bse1I ACTGG 6 cut(s) 324, 334, 409, 486, 561, 652
Bse8I GATNNNNATC 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 267, 603, 790
BseGI GGATG 2 cut(s) 38, 411
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 5 cut(s) 102, 151, 330, 581, 658
BseMII CTCAG 1 cut(s) 644
BseNI ACTGG 6 cut(s) 324, 334, 409, 486, 561, 652
BseRI GAGGAG 2 cut(s) 74, 516
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 1 cut(s) 706
BshFI GGCC 1 cut(s) 570
BshNI GGYRCC 2 cut(s) 305, 617
BsiSI CCGG 4 cut(s) 21, 103, 309, 691
BslI CCNNNNNNNGG 5 cut(s) 102, 151, 330, 581, 658
BsmAI GTCTC 4 cut(s) 257, 266, 286, 556
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 1 cut(s) 570
Bso31I GGTCTC 2 cut(s) 257, 266
Bsp1286I GDGCHC 2 cut(s) 112, 757
Bsp143I GATC 4 cut(s) 346, 358, 424, 589
BspACI CCGC 3 cut(s) 65, 68, 186
BspANI GGCC 1 cut(s) 570
BspCNI CTCAG 1 cut(s) 643
BspLI GGNNCC 3 cut(s) 202, 307, 619
BspPI GGATC 1 cut(s) 366
BspQI GCTCTTC 1 cut(s) 762
BspT107I GGYRCC 2 cut(s) 305, 617
BspTNI GGTCTC 2 cut(s) 257, 266
BsrI ACTGG 6 cut(s) 324, 334, 409, 486, 561, 652
BssECI CCNNGG 3 cut(s) 267, 603, 790
BssMI GATC 4 cut(s) 346, 358, 424, 589
BssNI GRCGYC 1 cut(s) 444
BssT1I CCWWGG 2 cut(s) 267, 790
Bst6I CTCTTC 2 cut(s) 233, 762
BstACI GRCGYC 1 cut(s) 444
BstC8I GCNNGC 1 cut(s) 699
BstDEI CTNAG 2 cut(s) 29, 630
BstDSI CCRYGG 1 cut(s) 603
BstF5I GGATG 2 cut(s) 38, 411
BstKTI GATC 4 cut(s) 349, 361, 427, 592
BstMAI GTCTC 4 cut(s) 257, 266, 286, 556
BstMBI GATC 4 cut(s) 346, 358, 424, 589
BstMWI GCNNNNNNNGC 3 cut(s) 30, 795, 804
BstNSI RCATGY 1 cut(s) 395
BstPAI GACNNNNGTC 2 cut(s) 278, 783
BstSCI CCNGG 2 cut(s) 19, 101
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 1 cut(s) 706
BstV2I GAAGAC 1 cut(s) 447
BsuRI GGCC 1 cut(s) 570
BtgI CCRYGG 1 cut(s) 603
BtrI CACGTC 2 cut(s) 388, 785
BtsCI GGATG 2 cut(s) 38, 411
BtsIMutI CAGTG 2 cut(s) 479, 554
Cac8I GCNNGC 1 cut(s) 699
Csp6I GTAC 2 cut(s) 327, 618
CviAII CATG 4 cut(s) 151, 392, 540, 548
CviQI GTAC 2 cut(s) 327, 618
DdeI CTNAG 2 cut(s) 29, 630
DpnI GATC 4 cut(s) 348, 360, 426, 591
DpnII GATC 4 cut(s) 346, 358, 424, 589
DraIII CACNNNGTG 1 cut(s) 485
EaeI YGGCCR 1 cut(s) 568
Eam1104I CTCTTC 2 cut(s) 233, 762
EarI CTCTTC 2 cut(s) 233, 762
EciI GGCGGA 2 cut(s) 54, 175
Eco130I CCWWGG 2 cut(s) 267, 790
Eco24I GRGCYC 1 cut(s) 757
Eco31I GGTCTC 2 cut(s) 257, 266
Eco57I CTGAAG 2 cut(s) 158, 578
EcoT14I CCWWGG 2 cut(s) 267, 790
EcoT38I GRGCYC 1 cut(s) 757
ErhI CCWWGG 2 cut(s) 267, 790
FaeI CATG 4 cut(s) 154, 395, 543, 551
FatI CATG 4 cut(s) 150, 391, 539, 547
FauNDI CATATG 1 cut(s) 351
FbaI TGATCA 2 cut(s) 346, 424
Fnu4HI GCNGC 2 cut(s) 68, 695
FokI GGATG 2 cut(s) 25, 398
FriOI GRGCYC 1 cut(s) 757
Fsp4HI GCNGC 2 cut(s) 68, 695
FspBI CTAG 1 cut(s) 770
GluI GCNGC 2 cut(s) 68, 695
GsuI CTGGAG 1 cut(s) 128
HaeIII GGCC 1 cut(s) 570
HapII CCGG 4 cut(s) 21, 103, 309, 691
Hin1I GRCGYC 1 cut(s) 444
Hin1II CATG 4 cut(s) 154, 395, 543, 551
HinfI GANTC 8 cut(s) 81, 155, 171, 218, 320, 334, 498, 544
HpaII CCGG 4 cut(s) 21, 103, 309, 691
HphI GGTGA 9 cut(s) 27, 36, 79, 202, 419, 497, 524, 673, 707
Hpy188I TCNGA 1 cut(s) 597
Hpy188III TCNNGA 2 cut(s) 530, 831
Hpy99I CGWCG 2 cut(s) 449, 789
HpyAV CCTTC 6 cut(s) 64, 100, 234, 349, 699, 745
HpyCH4IV ACGT 4 cut(s) 387, 444, 784, 826
HpyCH4V TGCA 2 cut(s) 391, 749
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 795, 804
HpyF3I CTNAG 2 cut(s) 29, 630
HpySE526I ACGT 4 cut(s) 387, 444, 784, 826
Hsp92I GRCGYC 1 cut(s) 444
Hsp92II CATG 4 cut(s) 154, 395, 543, 551
KpnI GGTACC 1 cut(s) 621
Ksp22I TGATCA 2 cut(s) 346, 424
Kzo9I GATC 4 cut(s) 346, 358, 424, 589
LguI GCTCTTC 1 cut(s) 762
LmnI GCTCC 4 cut(s) 147, 206, 529, 795
Lsp1109I GCAGC 1 cut(s) 706
MaeI CTAG 1 cut(s) 770
MaeII ACGT 4 cut(s) 387, 444, 784, 826
MaeIII GTNAC 3 cut(s) 407, 512, 822
MalI GATC 4 cut(s) 348, 360, 426, 591
MboI GATC 4 cut(s) 346, 358, 424, 589
MboII GAAGA 4 cut(s) 250, 452, 584, 749
MhlI GDGCHC 2 cut(s) 112, 757
MluCI AATT 3 cut(s) 97, 243, 312
MlyI GAGTC 2 cut(s) 75, 149
MmeI TCCRAC 2 cut(s) 336, 363
MnlI CCTC 9 cut(s) 52, 95, 193, 199, 244, 252, 390, 537, 721
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 2 cut(s) 420, 435
MspA1I CMGCKG 1 cut(s) 634
MspI CCGG 4 cut(s) 21, 103, 309, 691
MspR9I CCNGG 2 cut(s) 21, 103
Mva1269I GAATGC 1 cut(s) 798
MwoI GCNNNNNNNGC 3 cut(s) 30, 795, 804
NciI CCSGG 2 cut(s) 21, 103
NdeI CATATG 1 cut(s) 351
NdeII GATC 4 cut(s) 346, 358, 424, 589
NlaIII CATG 4 cut(s) 154, 395, 543, 551
NlaIV GGNNCC 3 cut(s) 202, 307, 619
NmuCI GTSAC 3 cut(s) 407, 512, 822
NspI RCATGY 1 cut(s) 395
PciSI GCTCTTC 1 cut(s) 762
PctI GAATGC 1 cut(s) 798
PfeI GAWTC 6 cut(s) 171, 218, 320, 334, 498, 544
PflMI CCANNNNNTGG 2 cut(s) 151, 658
PkrI GCNGC 2 cut(s) 69, 696
PleI GAGTC 2 cut(s) 75, 149
PpsI GAGTC 2 cut(s) 75, 149
PshAI GACNNNNGTC 2 cut(s) 278, 783
PspN4I GGNNCC 3 cut(s) 202, 307, 619
PvuII CAGCTG 1 cut(s) 634
RsaI GTAC 2 cut(s) 328, 619
RsaNI GTAC 2 cut(s) 327, 618
RseI CAYNNNNRTG 2 cut(s) 420, 435
SapI GCTCTTC 1 cut(s) 762
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 2 cut(s) 68, 695
Sau3AI GATC 4 cut(s) 346, 358, 424, 589
ScaI AGTACT 1 cut(s) 328
SchI GAGTC 2 cut(s) 75, 149
ScrFI CCNGG 2 cut(s) 21, 103
SduI GDGCHC 2 cut(s) 112, 757
SmiMI CAYNNNNRTG 2 cut(s) 420, 435
Sse9I AATT 3 cut(s) 97, 243, 312
SsiI CCGC 3 cut(s) 65, 68, 186
SspMI CTAG 1 cut(s) 770
StyD4I CCNGG 2 cut(s) 19, 101
StyI CCWWGG 2 cut(s) 267, 790
TaiI ACGT 4 cut(s) 390, 447, 787, 829
TaqI TCGA 2 cut(s) 760, 809
TasI AATT 3 cut(s) 97, 243, 312
TatI WGTACW 1 cut(s) 326
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 6 cut(s) 171, 218, 320, 334, 498, 544
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 2 cut(s) 486, 561
TseFI GTSAC 3 cut(s) 407, 512, 822
TseI GCWGC 1 cut(s) 694
Tsp45I GTSAC 3 cut(s) 407, 512, 822
TspDTI ATGAA 4 cut(s) 453, 536, 549, 674
TspGWI ACGGA 3 cut(s) 183, 274, 620
TspRI CASTG 2 cut(s) 486, 561
Van91I CCANNNNNTGG 2 cut(s) 151, 658
XceI RCATGY 1 cut(s) 395
XspI CTAG 1 cut(s) 770
ZraI GACGTC 1 cut(s) 445
ZrmI AGTACT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.