Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

17863011 .. 17864858

Gene Structure 1848 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 555 bp

ATGTATCAGGGTGTCCCGATGCTGTGTATGGGTGATGAATATGGCCATACAAAAGGGGGAAACAACAACACATATTGCCATGATAATTATATTAATTACTTTCGATGGGATAAGAAAGAAGAGTCCTCATCGGACTTTTTCAGATTTTGCTGCCTCATGACCAAATTCCGCCAGGAATGCGAGTCACTTGGGTTAAATGACTTCCCAACGGCAGAGAGGCTGCAGTGGCATGGTCATGCTCCTGGGGTGCCAGACTGGTCTGACACAAGCCGCTTTGTTGCCTTTACTCTGATGGACTCCGTGAAGAGAGAGCTGTATATCGCTTTCAATGCCACCCACTTAGCTGTGATCATTTCTCTGCCAGAGAGGCCTGGATACAGATGGGAGCCCTTGGTCGACACCAGCAAGCCTGCACCATTTGACTTCCTTACTAGCGACCTTCCAGAAAGAGATACCGCAGTTAAACAGTATGCTCACTTTCTCGACACCAATTTATATCCCATGCTAAGTTATTCTTCAGTCATCCTTTTACTTTCTCCCGTCGAAGATCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

185

Amino Acids

21.29

Weight (kDa)

5.03

Isoelectric Point (pI)

49.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ISOA1-3_C	PF21156	75 - 176	9e-48	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	247
AccI	GTMKAC	1 cut(s)	396
AciI	CCGC	3 cut(s)	169, 271, 456
AclWI	GGATC	1 cut(s)	542
AcoI	YGGCCR	1 cut(s)	43
AcsI	RAATTY	1 cut(s)	164
AcuI	CTGAAG	1 cut(s)	501
AgsI	TTSAA	1 cut(s)	328
AjnI	CCWGG	3 cut(s)	171, 241, 370
AluBI	AGCT	2 cut(s)	313, 344
AluI	AGCT	2 cut(s)	313, 344
AlwI	GGATC	1 cut(s)	542
AoxI	GGCC	2 cut(s)	43, 368
ApeKI	GCWGC	2 cut(s)	150, 220
ApoI	RAATTY	1 cut(s)	164
AseI	ATTAAT	1 cut(s)	93
AsuHPI	GGTGA	1 cut(s)	44
BaeI	ACNNNNGTAYC	2 cut(s)	444, 477
BalI	TGGCCA	1 cut(s)	45
BanI	GGYRCC	1 cut(s)	247
BanII	GRGCYC	1 cut(s)	390
BbvI	GCAGC	2 cut(s)	137, 207
BccI	CCATC	3 cut(s)	99, 286, 375
BceAI	ACGGC	1 cut(s)	225
BciT130I	CCWGG	3 cut(s)	173, 243, 372
BciVI	GTATCC	1 cut(s)	368
BclI	TGATCA	1 cut(s)	348
BfaI	CTAG	1 cut(s)	432
BfmI	CTRYAG	1 cut(s)	221
BfuI	GTATCC	1 cut(s)	368
BglI	GCCNNNNNGGC	1 cut(s)	367
BisI	GCNGC	3 cut(s)	151, 221, 271
BlsI	GCNGC	3 cut(s)	152, 222, 272
Bme1390I	CCNGG	3 cut(s)	173, 243, 372
BmiI	GGNNCC	2 cut(s)	249, 387
BmrFI	CCNGG	3 cut(s)	173, 243, 372
BmsI	GCATC	1 cut(s)	9
BsaJI	CCNNGG	2 cut(s)	242, 390
Bse1I	ACTGG	1 cut(s)	260
BseBI	CCWGG	3 cut(s)	173, 243, 372
BseDI	CCNNGG	2 cut(s)	242, 390
BseGI	GGATG	1 cut(s)	522
BseNI	ACTGG	1 cut(s)	260
BseXI	GCAGC	2 cut(s)	137, 207
BsgI	GTGCAG	1 cut(s)	396
BshFI	GGCC	2 cut(s)	45, 370
BshNI	GGYRCC	1 cut(s)	247
BsmI	GAATGC	1 cut(s)	182
BsnI	GGCC	2 cut(s)	45, 370
Bsp1286I	GDGCHC	1 cut(s)	390
Bsp143I	GATC	2 cut(s)	348, 547
BspACI	CCGC	3 cut(s)	169, 271, 456
BspANI	GGCC	2 cut(s)	45, 370
BspHI	TCATGA	1 cut(s)	156
BspLI	GGNNCC	2 cut(s)	249, 387
BspMAI	CTGCAG	1 cut(s)	225
BspPI	GGATC	1 cut(s)	542
BspT107I	GGYRCC	1 cut(s)	247
BsrI	ACTGG	1 cut(s)	260
BssECI	CCNNGG	2 cut(s)	242, 390
BssMI	GATC	2 cut(s)	348, 547
BssT1I	CCWWGG	1 cut(s)	390
Bst2UI	CCWGG	3 cut(s)	173, 243, 372
Bst4CI	ACNGT	1 cut(s)	468
Bst6I	CTCTTC	2 cut(s)	114, 299
BstC8I	GCNNGC	2 cut(s)	407, 411
BstDEI	CTNAG	2 cut(s)	340, 506
BstF5I	GGATG	1 cut(s)	522
BstKTI	GATC	2 cut(s)	351, 550
BstMBI	GATC	2 cut(s)	348, 547
BstMWI	GCNNNNNNNGC	4 cut(s)	177, 226, 329, 367
BstNI	CCWGG	3 cut(s)	173, 243, 372
BstSCI	CCNGG	3 cut(s)	171, 241, 370
BstSFI	CTRYAG	1 cut(s)	221
BstV1I	GCAGC	2 cut(s)	137, 207
BstX2I	RGATCY	1 cut(s)	547
BstYI	RGATCY	1 cut(s)	547
BsuI	GTATCC	1 cut(s)	368
BsuRI	GGCC	2 cut(s)	45, 370
BtsCI	GGATG	1 cut(s)	522
BtsI	GCAGTG	1 cut(s)	230
BtsIMutI	CAGTG	1 cut(s)	230
Cac8I	GCNNGC	2 cut(s)	407, 411
CciI	TCATGA	1 cut(s)	156
CviAII	CATG	5 cut(s)	80, 157, 230, 236, 502
CviJI	RGCY	8 cut(s)	45, 220, 270, 313, 344, 370, 388, 409
CviKI_1	RGCY	8 cut(s)	45, 220, 270, 313, 344, 370, 388, 409
DdeI	CTNAG	2 cut(s)	340, 506
DpnI	GATC	2 cut(s)	350, 549
DpnII	GATC	2 cut(s)	348, 547
EaeI	YGGCCR	1 cut(s)	43
Eam1104I	CTCTTC	2 cut(s)	114, 299
EarI	CTCTTC	2 cut(s)	114, 299
EciI	GGCGGA	1 cut(s)	158
Eco130I	CCWWGG	1 cut(s)	390
Eco147I	AGGCCT	1 cut(s)	370
Eco24I	GRGCYC	1 cut(s)	390
Eco57I	CTGAAG	1 cut(s)	501
EcoRII	CCWGG	3 cut(s)	171, 241, 370
EcoT14I	CCWWGG	1 cut(s)	390
EcoT38I	GRGCYC	1 cut(s)	390
ErhI	CCWWGG	1 cut(s)	390
FaeI	CATG	5 cut(s)	83, 160, 233, 239, 505
FalI	AAGNNNNNCTT	2 cut(s)	499, 531
FatI	CATG	5 cut(s)	79, 156, 229, 235, 501
FbaI	TGATCA	1 cut(s)	348
FblI	GTMKAC	1 cut(s)	396
Fnu4HI	GCNGC	3 cut(s)	151, 221, 271
FokI	GGATG	1 cut(s)	509
FriOI	GRGCYC	1 cut(s)	390
Fsp4HI	GCNGC	3 cut(s)	151, 221, 271
FspBI	CTAG	1 cut(s)	432
GluI	GCNGC	3 cut(s)	151, 221, 271
HaeIII	GGCC	2 cut(s)	45, 370
Hin1II	CATG	5 cut(s)	83, 160, 233, 239, 505
HincII	GTYRAC	1 cut(s)	397
HindII	GTYRAC	1 cut(s)	397
HinfI	GANTC	3 cut(s)	122, 182, 296
HphI	GGTGA	1 cut(s)	44
Hpy166II	GTNNAC	1 cut(s)	397
Hpy188I	TCNGA	4 cut(s)	133, 143, 262, 291
Hpy188III	TCNNGA	4 cut(s)	16, 157, 443, 482
Hpy8I	GTNNAC	1 cut(s)	397
Hpy99I	CGWCG	1 cut(s)	545
HpyAV	CCTTC	1 cut(s)	449
HpyCH4III	ACNGT	1 cut(s)	468
HpyCH4V	TGCA	2 cut(s)	223, 413
HpyF10VI	GCNNNNNNNGC	4 cut(s)	177, 226, 329, 367
HpyF3I	CTNAG	2 cut(s)	340, 506
Hsp92II	CATG	5 cut(s)	83, 160, 233, 239, 505
Ksp22I	TGATCA	1 cut(s)	348
Kzo9I	GATC	2 cut(s)	348, 547
LmnI	GCTCC	2 cut(s)	244, 385
Lsp1109I	GCAGC	2 cut(s)	137, 207
LweI	GCATC	1 cut(s)	9
MaeI	CTAG	1 cut(s)	432
MaeIII	GTNAC	1 cut(s)	183
MalI	GATC	2 cut(s)	350, 549
MboI	GATC	2 cut(s)	348, 547
MboII	GAAGA	3 cut(s)	131, 316, 507
MflI	RGATCY	1 cut(s)	547
MhlI	GDGCHC	1 cut(s)	390
MlsI	TGGCCA	1 cut(s)	45
MluCI	AATT	4 cut(s)	85, 94, 164, 490
MluNI	TGGCCA	1 cut(s)	45
MlyI	GAGTC	3 cut(s)	131, 191, 290
MnlI	CCTC	4 cut(s)	136, 164, 210, 360
Mox20I	TGGCCA	1 cut(s)	45
MscI	TGGCCA	1 cut(s)	45
MseI	TTAA	3 cut(s)	93, 194, 462
MslI	CAYNNNNRTG	1 cut(s)	234
Msp20I	TGGCCA	1 cut(s)	45
MspR9I	CCNGG	3 cut(s)	173, 243, 372
Mva1269I	GAATGC	1 cut(s)	182
MvaI	CCWGG	3 cut(s)	173, 243, 372
MwoI	GCNNNNNNNGC	4 cut(s)	177, 226, 329, 367
NdeII	GATC	2 cut(s)	348, 547
NlaIII	CATG	5 cut(s)	83, 160, 233, 239, 505
NlaIV	GGNNCC	2 cut(s)	249, 387
NmuCI	GTSAC	1 cut(s)	183
PagI	TCATGA	1 cut(s)	156
PceI	AGGCCT	1 cut(s)	370
PctI	GAATGC	1 cut(s)	182
PkrI	GCNGC	3 cut(s)	152, 222, 272
PleI	GAGTC	3 cut(s)	130, 190, 290
PpsI	GAGTC	3 cut(s)	130, 190, 290
PshBI	ATTAAT	1 cut(s)	93
Psp6I	CCWGG	3 cut(s)	171, 241, 370
PspGI	CCWGG	3 cut(s)	171, 241, 370
PspN4I	GGNNCC	2 cut(s)	249, 387
PstI	CTGCAG	1 cut(s)	225
PsuI	RGATCY	1 cut(s)	547
RseI	CAYNNNNRTG	1 cut(s)	234
SalI	GTCGAC	1 cut(s)	395
SaqAI	TTAA	3 cut(s)	93, 194, 462
SatI	GCNGC	3 cut(s)	151, 221, 271
Sau3AI	GATC	2 cut(s)	348, 547
SchI	GAGTC	3 cut(s)	131, 191, 290
ScrFI	CCNGG	3 cut(s)	173, 243, 372
SduI	GDGCHC	1 cut(s)	390
SetI	ASST	3 cut(s)	315, 346, 441
SfaNI	GCATC	1 cut(s)	9
SfcI	CTRYAG	1 cut(s)	221
SmiMI	CAYNNNNRTG	1 cut(s)	234
Sse9I	AATT	4 cut(s)	85, 94, 164, 490
SseBI	AGGCCT	1 cut(s)	370
SsiI	CCGC	3 cut(s)	169, 271, 456
SspMI	CTAG	1 cut(s)	432
StuI	AGGCCT	1 cut(s)	370
StyD4I	CCNGG	3 cut(s)	171, 241, 370
StyI	CCWWGG	1 cut(s)	390
TaaI	ACNGT	1 cut(s)	468
TaqI	TCGA	4 cut(s)	103, 396, 483, 543
TasI	AATT	4 cut(s)	85, 94, 164, 490
TauI	GCSGC	1 cut(s)	273
Tru1I	TTAA	3 cut(s)	93, 194, 462
Tru9I	TTAA	3 cut(s)	93, 194, 462
TscAI	CASTG	1 cut(s)	230
TseFI	GTSAC	1 cut(s)	183
TseI	GCWGC	2 cut(s)	150, 220
Tsp45I	GTSAC	1 cut(s)	183
TspDTI	ATGAA	1 cut(s)	51
TspGWI	ACGGA	1 cut(s)	289
TspRI	CASTG	1 cut(s)	230
VspI	ATTAAT	1 cut(s)	93
XapI	RAATTY	1 cut(s)	164
XmiI	GTMKAC	1 cut(s)	396
XspI	CTAG	1 cut(s)	432

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.