pycom16g26380

Uncharacterised protein family UPF0047

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010467
Physical Location & Seq
Forward (+)
28782710 .. 28784680
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 525 bp
ATGCAAATGCAACGCTCACTCGGTGCGAGTCTGAATCCGAGTAGAAGAGGCAGAGGCACCGCTGTCGATTCACTTCCAATGGCAGCCGCCGGTGGTCCTAAGTGGGCCCAGAAGACCATAACTCTGCCTCCCTTCAAGAGGGGTTGTCATCTCGTTACCCCTAAGATAGTGAAGGAAATTGGGGAGGACTTGTCAGACTTCAAGTGTGGCCTTGCCCATCTCTTCTTGCAGCACACAAGTGCTTCTCTTACTATCAATGAGAATTATGACTCTGATGTTCGTCACGATGCAGAGACATTCCTCAATACCATTGTTCCTGAGGGGACCTCTGCACCATGGAAGCATACCCTTGAAGGCCCAGATGACATGCCAGCACATATTAAATCATCAATGTTTGGCTGCACACTGACGGTTCCAATTACAAATGGAAAACTCAACATGGGAACATGGCAGGGAATATGGTTGTGTGAGCATCGTGACCATTCTACAGCACGGAAAGTTGTGGTTACCCTGAATGGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1 UPF0047

Protein Analysis

175

Amino Acids

19.03

Weight (kDa)

8.4

Isoelectric Point (pI)

29.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YjbQ PF01894 52 - 170 1.6e-40 UPF0047 protein YjbQ-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AciI CCGC 2 cut(s) 60, 87
AdeI CACNNNGTG 1 cut(s) 23
AfiI CCNNNNNNNGG 1 cut(s) 138
AgsI TTSAA 3 cut(s) 136, 202, 353
AleI CACNNNNGTG 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 287
AoxI GGCC 3 cut(s) 105, 208, 355
ApaI GGGCCC 1 cut(s) 109
ApeKI GCWGC 3 cut(s) 83, 229, 399
AspS9I GGNCC 5 cut(s) 95, 105, 106, 324, 356
AvaII GGWCC 2 cut(s) 95, 324
AxyI CCTNAGG 1 cut(s) 318
BaeGI GKGCMC 1 cut(s) 109
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 119
BbvI GCAGC 3 cut(s) 95, 241, 386
BccI CCATC 1 cut(s) 225
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BcoDI GTCTC 1 cut(s) 287
BfmI CTRYAG 1 cut(s) 486
BisI GCNGC 4 cut(s) 84, 87, 230, 400
BlsI GCNGC 4 cut(s) 85, 88, 231, 401
Bme18I GGWCC 2 cut(s) 95, 324
BmgT120I GGNCC 5 cut(s) 95, 105, 106, 324, 356
BmiI GGNNCC 4 cut(s) 58, 107, 325, 414
BmsI GCATC 2 cut(s) 277, 481
BpiI GAAGAC 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BsaJI CCNNGG 1 cut(s) 335
BsaXI ACNNNNNCTCC 4 cut(s) 112, 142, 176, 206
Bsc4I CCNNNNNNNGG 1 cut(s) 138
Bse118I RCCGGY 1 cut(s) 89
Bse21I CCTNAGG 1 cut(s) 318
BseDI CCNNGG 1 cut(s) 335
BseLI CCNNNNNNNGG 1 cut(s) 138
BseMII CTCAG 1 cut(s) 309
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 3 cut(s) 95, 241, 386
BsgI GTGCAG 2 cut(s) 315, 385
BshFI GGCC 3 cut(s) 107, 210, 357
BshNI GGYRCC 1 cut(s) 56
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 337
BsnI GGCC 3 cut(s) 107, 210, 357
Bsp120I GGGCCC 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 109
Bsp19I CCATGG 1 cut(s) 335
BspACI CCGC 2 cut(s) 60, 87
BspANI GGCC 3 cut(s) 107, 210, 357
BspCNI CTCAG 1 cut(s) 310
BspLI GGNNCC 4 cut(s) 58, 107, 325, 414
BspT107I GGYRCC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 335
BssT1I CCWWGG 1 cut(s) 335
Bst4CI ACNGT 1 cut(s) 412
Bst6I CTCTTC 2 cut(s) 40, 227
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 3 cut(s) 99, 162, 318
BstDSI CCRYGG 1 cut(s) 335
BstEII GGTNACC 1 cut(s) 505
BstENI CCTNNNNNAGG 1 cut(s) 136
BstMAI GTCTC 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 370
BstPI GGTNACC 1 cut(s) 505
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 109
BstV1I GCAGC 3 cut(s) 95, 241, 386
BstV2I GAAGAC 1 cut(s) 119
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 3 cut(s) 107, 210, 357
BtgI CCRYGG 1 cut(s) 335
BtsIMutI CAGTG 1 cut(s) 404
Cac8I GCNNGC 1 cut(s) 372
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 5 cut(s) 95, 105, 106, 324, 356
CviAII CATG 4 cut(s) 336, 367, 439, 447
CviJI RGCY 5 cut(s) 86, 107, 210, 357, 399
CviKI_1 RGCY 5 cut(s) 86, 107, 210, 357, 399
DdeI CTNAG 3 cut(s) 99, 162, 318
DraIII CACNNNGTG 1 cut(s) 23
Eam1104I CTCTTC 2 cut(s) 40, 227
EarI CTCTTC 2 cut(s) 40, 227
Eco130I CCWWGG 1 cut(s) 335
Eco24I GRGCYC 1 cut(s) 109
Eco47I GGWCC 2 cut(s) 95, 324
Eco81I CCTNAGG 1 cut(s) 318
Eco91I GGTNACC 1 cut(s) 505
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 324
EcoO65I GGTNACC 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 335
EcoT38I GRGCYC 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 335
FaeI CATG 4 cut(s) 339, 370, 442, 450
FaqI GGGAC 1 cut(s) 337
FatI CATG 4 cut(s) 335, 366, 438, 446
Fnu4HI GCNGC 4 cut(s) 84, 87, 230, 400
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 4 cut(s) 84, 87, 230, 400
GluI GCNGC 4 cut(s) 84, 87, 230, 400
HaeIII GGCC 3 cut(s) 107, 210, 357
HapII CCGG 1 cut(s) 90
Hin1II CATG 4 cut(s) 339, 370, 442, 450
HinfI GANTC 4 cut(s) 28, 34, 68, 269
HpaII CCGG 1 cut(s) 90
Hpy188I TCNGA 4 cut(s) 33, 39, 196, 274
Hpy188III TCNNGA 4 cut(s) 136, 284, 317, 476
HpyAV CCTTC 3 cut(s) 142, 166, 347
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4V TGCA 6 cut(s) 4, 10, 229, 290, 332, 402
HpyF3I CTNAG 3 cut(s) 99, 162, 318
Hsp92II CATG 4 cut(s) 339, 370, 442, 450
LpnPI CCDG 6 cut(s) 103, 122, 330, 372, 384, 437
Lsp1109I GCAGC 3 cut(s) 95, 241, 386
LweI GCATC 2 cut(s) 277, 481
MaeIII GTNAC 4 cut(s) 154, 281, 476, 505
MboII GAAGA 3 cut(s) 57, 124, 214
MhlI GDGCHC 1 cut(s) 109
MluCI AATT 3 cut(s) 177, 262, 417
MlyI GAGTC 2 cut(s) 37, 263
MnlI CCTC 8 cut(s) 41, 47, 132, 138, 178, 311, 313, 337
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 237
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 1 cut(s) 90
NcoI CCATGG 1 cut(s) 335
NlaIII CATG 4 cut(s) 339, 370, 442, 450
NlaIV GGNNCC 4 cut(s) 58, 107, 325, 414
NmuCI GTSAC 2 cut(s) 281, 476
NspI RCATGY 1 cut(s) 370
OliI CACNNNNGTG 1 cut(s) 237
PfeI GAWTC 2 cut(s) 34, 68
PkrI GCNGC 4 cut(s) 85, 88, 231, 401
PleI GAGTC 2 cut(s) 36, 263
PpsI GAGTC 2 cut(s) 36, 263
PpuMI RGGWCCY 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 324
PspEI GGTNACC 1 cut(s) 505
PspN4I GGNNCC 4 cut(s) 58, 107, 325, 414
PspOMI GGGCCC 1 cut(s) 105
PspPI GGNCC 5 cut(s) 95, 105, 106, 324, 356
PspPPI RGGWCCY 1 cut(s) 324
RseI CAYNNNNRTG 1 cut(s) 237
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 4 cut(s) 84, 87, 230, 400
Sau96I GGNCC 5 cut(s) 95, 105, 106, 324, 356
SchI GAGTC 2 cut(s) 37, 263
SduI GDGCHC 1 cut(s) 109
SetI ASST 1 cut(s) 329
SfaNI GCATC 2 cut(s) 277, 481
SfcI CTRYAG 1 cut(s) 486
SgrAI CRCCGGYG 1 cut(s) 89
SinI GGWCC 2 cut(s) 95, 324
SmiMI CAYNNNNRTG 1 cut(s) 237
Sse9I AATT 3 cut(s) 177, 262, 417
SsiI CCGC 2 cut(s) 60, 87
StyI CCWWGG 1 cut(s) 335
TaaI ACNGT 1 cut(s) 412
TaqI TCGA 1 cut(s) 66
TasI AATT 3 cut(s) 177, 262, 417
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 2 cut(s) 34, 68
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 1 cut(s) 411
TseFI GTSAC 2 cut(s) 281, 476
TseI GCWGC 3 cut(s) 83, 229, 399
Tsp45I GTSAC 2 cut(s) 281, 476
TspGWI ACGGA 1 cut(s) 508
TspRI CASTG 1 cut(s) 411
VpaK11BI GGWCC 2 cut(s) 95, 324
XagI CCTNNNNNAGG 1 cut(s) 136
XceI RCATGY 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.