pycom17g02740

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004149
Physical Location & Seq
Reverse (-)
1827759 .. 1830637
2879 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1659 bp
ATGGATCTGTCGCAGGGATCAGCGCAGCAGCAGCACCACCAGAACGACGACTTCATCAACCTGATGAAGATTGACAGCAACACCAGCAACAGCCACCGCCAAGGCGGAGGAGAAGAAGAGTATCAGGTCGGCGGGGGCGGCCGTGGAAGTGGGATTTACAACAAGGAGAATCAGGAGATGCTTCCCAGCTACGATTTCCAGCCGATTCGCCCCGTCGTCGTTGCTTCTTCTCTGTCGCCGAGTTTCGATGCCGCGCCCAATCTTGGAGGAGGAGGACCGACCAGGGCGTGGAACTCCAGCGAGTCCAAGTCGAACACTGCATCTCCAATTAGAAATTATGGATCAATGGATTCCCTTGAACCTGCAAAACTTGTATTAGAGAAGGACCGCAATGCCGTAATTCTGTCAGAGATTGACCAGACCATGAAGAAGCATGCTGATAATTTGCTACATGTGCTGGAAGGTGTTAGTGCACGACTAACACAACTAGAGAGCAGAAGCCGCAATGTTGAGGATTCTGTCAATGATTTGAAGGGTATTCTTAGAGATGTGCAAACAGATGTTAAGGATTTGAAGGATGCGCAAGAAATGGTAGAATTGAATCTTGCAAGGATACAAGTATCAAATACAAAGATAGACCCTCAACTGGAACCTCAGAATGCTGGACAACGACATACTGAGCAGACAGCCGCCTCTGCTCCTCAACATTCTCAGCAACAAATTCATCCCCCCATGAACCTTCCTCCCTCACTTCCTGGTGTTTCTCATCCAAATGCTCCTCCCCAACCTATCCCCCAAAATGTACCACATGCAGTTCAACTTCCAAATCAGTTTTCTCAGAACCAGATCCCTCCCGTCCCTCAGAGAGACCCTTATTTCCCAGCGCCTGGTCAAACTCAAGAAGCCCCAAATCATCAATACCAATTACCTCCAGGTCAGCAGCCTGCTCCCCTTCCTCCAGTGCCACCGCATCAACAATTTCAACCTACCACTCAACCACAGTATTCCCAGCCGCCACCCCAGCTGCCTCAACAGCACCCTTCGCTTGCACCTGTTAATCCCTCTCAAATCCAGCCTACATTAGGCCACCACACTGAAGAGTCGCCTTATATCCCTTCCCAGAACTACCCACCAATTCTTCGCCAGCCACCTTCTCATACACCCAGCGGTCACCCTCCTTCCCAACAGTATTACAGTCCAGCTTCAAATGCATATGAGCCACCTTCTAGCAGACCCAGTGCAGGGTATCCTTCTGGGTATAACCCTCCATCTGGGCTTGGTGAGTCATACCAATACGGTGGACCACCTTCTCAATATGGCGGTAGCTCCTCAATGAAACCACAACATCACTCTTCTTCTGCTACAGCTCAAAGTGGAGGCAGTGGTTACCCACAGCTCCCAACTGCTCGGGTGCTACCACATGCCAACCCTACGCCATCTGGGGCGGGTGGCAGTTCACCTTCATCCGGTAGTGGAAGCAGGGTAGCCATTGACGATGTGATTGACCAAGTGGCAACTATGGGTTTCTCCAGAGACCAAGTTAGGGCTGCAGTTCGGAAGCTGACAGACAGTGGCCAGTCAGTTGACCTCAATGTGGTGCTAGATAAGTTGATGAACGACGGGGATGTTCAGCCGCCCCGAGGTTGGTATGGCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
DUF1421

Protein Analysis

553

Amino Acids

59.5

Weight (kDa)

5.9

Isoelectric Point (pI)

64.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 497 - 540 5.7e-18 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 582
Acc36I ACCTGC 1 cut(s) 370
AccB7I CCANNNNNTGG 2 cut(s) 288, 887
AccII CGCG 1 cut(s) 254
AclWI GGATC 4 cut(s) 12, 25, 349, 841
AcoI YGGCCR 3 cut(s) 139, 1573, 1651
AcsI RAATTY 1 cut(s) 720
AcuI CTGAAG 1 cut(s) 1116
AfaI GTAC 1 cut(s) 804
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 7 cut(s) 359, 532, 574, 601, 818, 983, 1206
AjnI CCWGG 4 cut(s) 281, 754, 886, 931
AjuI GAANNNNNNNTTGG 2 cut(s) 817, 849
AluBI AGCT 7 cut(s) 189, 1024, 1202, 1326, 1367, 1396, 1561
AluI AGCT 7 cut(s) 189, 1024, 1202, 1326, 1367, 1396, 1561
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 2 cut(s) 861, 1527
Alw44I GTGCAC 1 cut(s) 471
AlwI GGATC 4 cut(s) 12, 25, 349, 841
AlwNI CAGNNNCTG 1 cut(s) 887
Ama87I CYCGRG 2 cut(s) 1407, 1638
AoxI GGCC 4 cut(s) 139, 1084, 1573, 1651
ApaLI GTGCAC 1 cut(s) 471
ApeKI GCWGC 6 cut(s) 25, 28, 31, 940, 1024, 1547
ApoI RAATTY 1 cut(s) 720
AspLEI GCGC 4 cut(s) 25, 256, 583, 886
AspS9I GGNCC 3 cut(s) 275, 385, 1301
AsuHPI GGTGA 3 cut(s) 1163, 1292, 1449
AvaI CYCGRG 2 cut(s) 1407, 1638
AvaII GGWCC 3 cut(s) 275, 385, 1301
BaeGI GKGCMC 1 cut(s) 475
BalI TGGCCA 1 cut(s) 1575
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 6 cut(s) 37, 40, 43, 952, 1011, 1534
BccI CCATC 2 cut(s) 1276, 1444
BceAI ACGGC 2 cut(s) 126, 380
BciT130I CCWGG 4 cut(s) 283, 756, 888, 933
BciVI GTATCC 2 cut(s) 606, 1257
BcoDI GTCTC 2 cut(s) 861, 1527
BfaI CTAG 3 cut(s) 488, 1227, 1601
BfmI CTRYAG 2 cut(s) 1362, 1548
BfoI RGCGCY 1 cut(s) 887
BfuAI ACCTGC 1 cut(s) 370
BfuI GTATCC 2 cut(s) 606, 1257
Bme1390I CCNGG 4 cut(s) 283, 756, 888, 933
Bme18I GGWCC 3 cut(s) 275, 385, 1301
BmeT110I CYCGRG 2 cut(s) 1407, 1638
BmgT120I GGNCC 3 cut(s) 275, 385, 1301
BmiI GGNNCC 1 cut(s) 651
BmrFI CCNGG 4 cut(s) 283, 756, 888, 933
BmrI ACTGGG 1 cut(s) 1230
BmsI GCATC 5 cut(s) 168, 238, 329, 568, 979
BmuI ACTGGG 1 cut(s) 1230
BpmI CTGGAG 4 cut(s) 280, 915, 942, 1513
BpuEI CTTGAG 1 cut(s) 882
BsaI GGTCTC 2 cut(s) 861, 1527
BsaJI CCNNGG 4 cut(s) 100, 142, 282, 1639
BsaWI WCCGGW 1 cut(s) 1466
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bse1I ACTGG 4 cut(s) 651, 959, 1236, 1576
Bse3DI GCAATG 2 cut(s) 397, 511
BseBI CCWGG 4 cut(s) 283, 756, 888, 933
BseDI CCNNGG 4 cut(s) 100, 142, 282, 1639
BseGI GGATG 5 cut(s) 583, 724, 766, 1463, 1630
BseMI GCAATG 2 cut(s) 397, 511
BseMII CTCAG 5 cut(s) 668, 669, 725, 851, 875
BseNI ACTGG 4 cut(s) 651, 959, 1236, 1576
BseRI GAGGAG 6 cut(s) 123, 282, 285, 690, 768, 1318
BseSI GKGCMC 1 cut(s) 475
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 6 cut(s) 37, 40, 43, 952, 1011, 1534
BseYI CCCAGC 5 cut(s) 185, 880, 1008, 1020, 1163
BsgI GTGCAG 1 cut(s) 1260
Bsh1236I CGCG 1 cut(s) 254
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 4 cut(s) 141, 1086, 1575, 1653
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 475
BsiHKCI CYCGRG 2 cut(s) 1407, 1638
BsiSI CCGG 1 cut(s) 1467
BslFI GGGAC 1 cut(s) 842
BsmAI GTCTC 2 cut(s) 861, 1527
BsmFI GGGAC 1 cut(s) 842
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 4 cut(s) 141, 1086, 1575, 1653
Bso31I GGTCTC 2 cut(s) 861, 1527
BsoBI CYCGRG 2 cut(s) 1407, 1638
Bsp1286I GDGCHC 1 cut(s) 475
Bsp143I GATC 4 cut(s) 4, 17, 341, 846
BspANI GGCC 4 cut(s) 141, 1086, 1575, 1653
BspCNI CTCAG 5 cut(s) 667, 670, 724, 850, 874
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 1 cut(s) 651
BspMAI CTGCAG 1 cut(s) 1552
BspMI ACCTGC 1 cut(s) 370
BspPI GGATC 4 cut(s) 12, 25, 349, 841
BspTNI GGTCTC 2 cut(s) 861, 1527
BsrDI GCAATG 2 cut(s) 397, 511
BsrI ACTGG 4 cut(s) 651, 959, 1236, 1576
BssECI CCNNGG 4 cut(s) 100, 142, 282, 1639
BssMI GATC 4 cut(s) 4, 17, 341, 846
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 4 cut(s) 283, 756, 888, 933
Bst4CI ACNGT 5 cut(s) 1002, 1188, 1196, 1298, 1571
Bst6I CTCTTC 3 cut(s) 111, 1092, 1357
BstC8I GCNNGC 4 cut(s) 435, 945, 1047, 1145
BstDEI CTNAG 6 cut(s) 542, 654, 678, 711, 837, 861
BstDSI CCRYGG 1 cut(s) 142
BstEII GGTNACC 2 cut(s) 1169, 1385
BstENI CCTNNNNNAGG 1 cut(s) 1080
BstF5I GGATG 5 cut(s) 583, 724, 766, 1463, 1630
BstFNI CGCG 1 cut(s) 254
BstH2I RGCGCY 1 cut(s) 887
BstHHI GCGC 4 cut(s) 25, 256, 583, 886
BstKTI GATC 4 cut(s) 7, 20, 344, 849
BstMAI GTCTC 2 cut(s) 861, 1527
BstMBI GATC 4 cut(s) 4, 17, 341, 846
BstMCI CGRYCG 1 cut(s) 142
BstNI CCWGG 4 cut(s) 283, 756, 888, 933
BstNSI RCATGY 4 cut(s) 437, 455, 812, 1424
BstPI GGTNACC 2 cut(s) 1169, 1385
BstSCI CCNGG 4 cut(s) 281, 754, 886, 931
BstSFI CTRYAG 2 cut(s) 1362, 1548
BstSLI GKGCMC 1 cut(s) 475
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 6 cut(s) 37, 40, 43, 952, 1011, 1534
BstX2I RGATCY 2 cut(s) 4, 846
BstXI CCANNNNNNTGG 1 cut(s) 1298
BstYI RGATCY 2 cut(s) 4, 846
BstZI CGGCCG 1 cut(s) 139
BsuI GTATCC 2 cut(s) 606, 1257
BsuRI GGCC 4 cut(s) 141, 1086, 1575, 1653
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 5 cut(s) 583, 724, 766, 1463, 1630
BtsI GCAGTG 2 cut(s) 315, 1387
BtsIMutI CAGTG 6 cut(s) 315, 966, 1092, 1243, 1387, 1576
BveI ACCTGC 1 cut(s) 370
Cac8I GCNNGC 4 cut(s) 435, 945, 1047, 1145
CaiI CAGNNNCTG 1 cut(s) 887
CfoI GCGC 4 cut(s) 25, 256, 583, 886
Cfr13I GGNCC 3 cut(s) 275, 385, 1301
Csp6I GTAC 1 cut(s) 803
CviAII CATG 6 cut(s) 424, 434, 452, 733, 809, 1421
CviQI GTAC 1 cut(s) 803
DdeI CTNAG 6 cut(s) 542, 654, 678, 711, 837, 861
DpnI GATC 4 cut(s) 6, 19, 343, 848
DpnII GATC 4 cut(s) 4, 17, 341, 846
EaeI YGGCCR 3 cut(s) 139, 1573, 1651
EagI CGGCCG 1 cut(s) 139
Eam1104I CTCTTC 3 cut(s) 111, 1092, 1357
EarI CTCTTC 3 cut(s) 111, 1092, 1357
EciI GGCGGA 1 cut(s) 120
EclXI CGGCCG 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 100
Eco31I GGTCTC 2 cut(s) 861, 1527
Eco47I GGWCC 3 cut(s) 275, 385, 1301
Eco52I CGGCCG 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 1116
Eco88I CYCGRG 2 cut(s) 1407, 1638
Eco91I GGTNACC 2 cut(s) 1169, 1385
EcoNI CCTNNNNNAGG 1 cut(s) 1080
EcoO65I GGTNACC 2 cut(s) 1169, 1385
EcoRII CCWGG 4 cut(s) 281, 754, 886, 931
EcoT14I CCWWGG 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 1213
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 6 cut(s) 427, 437, 455, 736, 812, 1424
FaqI GGGAC 1 cut(s) 842
FatI CATG 6 cut(s) 423, 433, 451, 732, 808, 1420
FauI CCCGC 2 cut(s) 125, 1438
FauNDI CATATG 1 cut(s) 1213
FokI GGATG 5 cut(s) 590, 711, 753, 1450, 1637
FspBI CTAG 3 cut(s) 488, 1227, 1601
FspI TGCGCA 1 cut(s) 582
GlaI GCGC 4 cut(s) 24, 255, 582, 885
GsaI CCCAGC 5 cut(s) 189, 884, 1012, 1024, 1167
GsuI CTGGAG 4 cut(s) 280, 915, 942, 1513
HaeII RGCGCY 1 cut(s) 887
HaeIII GGCC 4 cut(s) 141, 1086, 1575, 1653
HapII CCGG 1 cut(s) 1467
HhaI GCGC 4 cut(s) 25, 256, 583, 886
Hin1II CATG 6 cut(s) 427, 437, 455, 736, 812, 1424
Hin6I GCGC 4 cut(s) 23, 254, 581, 884
HinP1I GCGC 4 cut(s) 23, 254, 581, 884
HincII GTYRAC 1 cut(s) 1585
HindII GTYRAC 1 cut(s) 1585
HinfI GANTC 8 cut(s) 169, 205, 302, 350, 515, 601, 1100, 1283
HpaII CCGG 1 cut(s) 1467
HphI GGTGA 3 cut(s) 1163, 1292, 1449
Hpy166II GTNNAC 4 cut(s) 473, 1301, 1457, 1585
Hpy188I TCNGA 5 cut(s) 409, 657, 840, 864, 1557
Hpy188III TCNNGA 3 cut(s) 173, 899, 1530
Hpy8I GTNNAC 4 cut(s) 473, 1301, 1457, 1585
Hpy99I CGWCG 4 cut(s) 50, 218, 221, 1622
HpyCH4III ACNGT 5 cut(s) 1002, 1188, 1196, 1298, 1571
HpyF3I CTNAG 6 cut(s) 542, 654, 678, 711, 837, 861
Hsp92II CATG 6 cut(s) 427, 437, 455, 736, 812, 1424
HspAI GCGC 4 cut(s) 23, 254, 581, 884
Kzo9I GATC 4 cut(s) 4, 17, 341, 846
LmnI GCTCC 5 cut(s) 703, 781, 952, 1331, 1401
Lsp1109I GCAGC 6 cut(s) 37, 40, 43, 952, 1011, 1534
LweI GCATC 5 cut(s) 168, 238, 329, 568, 979
MaeI CTAG 3 cut(s) 488, 1227, 1601
MaeIII GTNAC 2 cut(s) 1169, 1385
MalI GATC 4 cut(s) 6, 19, 343, 848
MboI GATC 4 cut(s) 4, 17, 341, 846
MboII GAAGA 9 cut(s) 79, 125, 128, 219, 439, 1109, 1130, 1344, 1347
MflI RGATCY 2 cut(s) 4, 846
MhlI GDGCHC 1 cut(s) 475
MlsI TGGCCA 1 cut(s) 1575
MluCI AATT 9 cut(s) 327, 334, 399, 442, 596, 720, 923, 977, 1134
MluNI TGGCCA 1 cut(s) 1575
MlyI GAGTC 3 cut(s) 311, 1109, 1292
Mox20I TGGCCA 1 cut(s) 1575
Mph1103I ATGCAT 1 cut(s) 1213
MscI TGGCCA 1 cut(s) 1575
MseI TTAA 2 cut(s) 564, 1056
MslI CAYNNNNRTG 1 cut(s) 771
Msp20I TGGCCA 1 cut(s) 1575
MspA1I CMGCKG 2 cut(s) 1024, 1167
MspI CCGG 1 cut(s) 1467
MspR9I CCNGG 4 cut(s) 283, 756, 888, 933
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 4 cut(s) 283, 756, 888, 933
MvnI CGCG 1 cut(s) 254
NdeI CATATG 1 cut(s) 1213
NdeII GATC 4 cut(s) 4, 17, 341, 846
NlaIII CATG 6 cut(s) 427, 437, 455, 736, 812, 1424
NlaIV GGNNCC 1 cut(s) 651
NmeAIII GCCGAG 1 cut(s) 264
NmuCI GTSAC 1 cut(s) 1169
NsbI TGCGCA 1 cut(s) 582
NsiI ATGCAT 1 cut(s) 1213
NspI RCATGY 4 cut(s) 437, 455, 812, 1424
PaeI GCATGC 1 cut(s) 437
PciI ACATGT 1 cut(s) 451
PctI GAATGC 1 cut(s) 664
PfeI GAWTC 5 cut(s) 169, 205, 350, 515, 601
PflMI CCANNNNNTGG 2 cut(s) 288, 887
PleI GAGTC 3 cut(s) 310, 1108, 1291
PpsI GAGTC 3 cut(s) 310, 1108, 1291
PscI ACATGT 1 cut(s) 451
Psp6I CCWGG 4 cut(s) 281, 754, 886, 931
PspEI GGTNACC 2 cut(s) 1169, 1385
PspFI CCCAGC 5 cut(s) 185, 880, 1008, 1020, 1163
PspGI CCWGG 4 cut(s) 281, 754, 886, 931
PspN4I GGNNCC 1 cut(s) 651
PspPI GGNCC 3 cut(s) 275, 385, 1301
PstI CTGCAG 1 cut(s) 1552
PstNI CAGNNNCTG 1 cut(s) 887
PsuI RGATCY 2 cut(s) 4, 846
PvuII CAGCTG 1 cut(s) 1024
RsaI GTAC 1 cut(s) 804
RsaNI GTAC 1 cut(s) 803
RseI CAYNNNNRTG 1 cut(s) 771
SaqAI TTAA 2 cut(s) 564, 1056
Sau3AI GATC 4 cut(s) 4, 17, 341, 846
Sau96I GGNCC 3 cut(s) 275, 385, 1301
SchI GAGTC 3 cut(s) 311, 1109, 1292
ScrFI CCNGG 4 cut(s) 283, 756, 888, 933
SduI GDGCHC 1 cut(s) 475
SfaNI GCATC 5 cut(s) 168, 238, 329, 568, 979
SfcI CTRYAG 2 cut(s) 1362, 1548
SinI GGWCC 3 cut(s) 275, 385, 1301
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 897
SmoI CTYRAG 1 cut(s) 897
SphI GCATGC 1 cut(s) 437
Sse9I AATT 9 cut(s) 327, 334, 399, 442, 596, 720, 923, 977, 1134
SspMI CTAG 3 cut(s) 488, 1227, 1601
StyD4I CCNGG 4 cut(s) 281, 754, 886, 931
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 5 cut(s) 1002, 1188, 1196, 1298, 1571
TaqI TCGA 2 cut(s) 246, 311
TaqII GACCGA 1 cut(s) 292
TasI AATT 9 cut(s) 327, 334, 399, 442, 596, 720, 923, 977, 1134
TauI GCSGC 6 cut(s) 141, 254, 504, 692, 1015, 1636
TfiI GAWTC 5 cut(s) 169, 205, 350, 515, 601
Tru1I TTAA 2 cut(s) 564, 1056
Tru9I TTAA 2 cut(s) 564, 1056
TscAI CASTG 6 cut(s) 322, 966, 1099, 1243, 1387, 1576
TseFI GTSAC 1 cut(s) 1169
TseI GCWGC 6 cut(s) 25, 28, 31, 940, 1024, 1547
Tsp45I GTSAC 1 cut(s) 1169
TspDTI ATGAA 8 cut(s) 43, 80, 440, 713, 749, 1349, 1452, 1628
TspRI CASTG 6 cut(s) 322, 966, 1099, 1243, 1387, 1576
Van91I CCANNNNNTGG 2 cut(s) 288, 887
VneI GTGCAC 1 cut(s) 471
VpaK11BI GGWCC 3 cut(s) 275, 385, 1301
XagI CCTNNNNNAGG 1 cut(s) 1080
XapI RAATTY 1 cut(s) 720
XceI RCATGY 4 cut(s) 437, 455, 812, 1424
XspI CTAG 3 cut(s) 488, 1227, 1601
Zsp2I ATGCAT 1 cut(s) 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.