pycom17g06120

longifolia

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004546
Physical Location & Seq
Reverse (-)
4308828 .. 4312041
3214 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2886 bp
ATGTATTTATATATTTTTACTTTTTTTTTCAGGGAAAAAATTCGAAAAAAGGTGGTGAAAGAGAAACAACGAAACTCCACAGAATCATCAAAGACCACTGTTTCTTCTTCGCCGTGTTCATGTAGCTTCTCGTCTCTTGAACATCAAAAGGCAGCTGAGCAAGAACCATCTTTGTCCAGCCGAACCATTTCCAACGACAAACACGCTCGGGACCTATCTCTGAACCAACCAAATGTTTTGATGCACATACGCCGGCAATCTGTTGATCTGCAAGATCTTGTCAAAGACTCAACGTACAGAGAAGCCCGTGGTATTTCAGTTAAACCTGCAGCTAAAGATGGAGCTGGTCATGCATTGACGTACATTGACTCCCCGAGGCCTTCACCAATAAAATCCACCAACCCAAGGGTTTCTGGTGTTAATGAGTCATTTCAAGTTCCTGGAAAGCTTCGGGTCGCACCTTGGAGTTCAAATGAAGAGAAGGACGGTGGCACGCGTTTTGTACCAAAAGATGCTCGGAGGTTCTCTTATGATGGAAGAGAGTCACGCGATGCATCAAAATCCACCGTAAAACTCAGAGAAATCCCAAGGCTTTCTTTGGACAGCAAAGAACGTTCCACAAGGAGGGGTTGTAACCCAGAAACAAAAGCAAATTACTTCATCAAGGATCTGCAGAGAGAATATGGGAACAGCAACAAAATGCTTGACCTGCAGCATGAACCTGGAAGCGCTAAAAGACCGTCTAATGTAGTTGCAAAGTTGATGGGACTGGATCTATCAGATTCCGTGTCAACCATTGATAGTCCATTAAGGCTGATTAATACTGGCACATCTGATAGATCTGATCCCTTCTCCAGATCATCAAGAGCAACCACTGAAAACAAGCAAGATATTTTTTCCGGAATACTCCTTGTGAATGCCAAGAAAGACTTCAGCTCACCCCAGAGGAGAAGTACCGATTCAGTCATGAAGCCCACCTCGAATTCTAAGTTTCCAATAGAAACAGCTCCTTGGAGGCAGCCGCACAGAAGCAAAGGTTCTCAACGATCAGCTTTCAAGCATCAAGAAGAACCGACAAAAGCTCCAAACTCATCATCTTCAGTATATGGTGAAATGGAGAAAAGGCTAGCAGATCTTGAGTTTAAAAAATCAGGAAAGGATCTCAGAGCTCTTAAACAAATACTTGAAGCGATGCAGAAGACGAAAGGAATGACAGATACAAGAAAGATCGATCACGCCTTAAATTTTGCATCTCAAATAAGCAACGAAAGCATTTCCTCTGACAGCACAGTAAAAGCAAGCCAGAGAAATCCACAAAGCAAAATATCAGTTCCTGCCACGGCCAAGGGTTCCCTATCTCCAAAGAGTTATAAATCACCCATCGTCATAATGAAACCAGCTAAACTCATTGATAAAACCCACAGCTCTGCTTCCATGGTGGATTCCATGAATGACACGTTAGGTCTTCGGAAGCTTCGGACTAGTGATCCTGGTGGTAACAGAAAGGGATTGGTTGACAAGAATACAGCCAAAGACCCGACACCAAGGAACAATCATGTCAGAGATCCTTTCAATCGACACCTTCATTCCATTGATGATAATTCTAACGTAAGAACTTTGAAATCTGCACAAAAACCAAAGGCATCGCAAACTATGAGTGAAGAAAAAGTCATCAGCTCAAGTAGGAGTTCAAAAATCACGAGCCCGAAAATGCAACAGAGAAGACTTGGATTGGAGAAGCAGTCTCCTCCAAGCACGCCATCATTGAATTCAAGCATGACCAGAAGACAACACACTAGGCAGTCATTAGAAGCAACCACCCCAGGTAGAAAACTTGGGCATAAGACACCCAGTTTGCATCAAAGTAATGCGCAACTGAGGGAGGCTAGCACCAAGATGAGAGAGATGAGTCATCACGATGATTCCACTTCACAGCAATCTGGAAGCACCATCAGCTTGACCTCTCACACAGACACAGCCACAAGCGTTCACCAATCAGATAGGATCTCACGCACGCATTTCCAGCATAACCAAAAAATAAAGCACCCAGCAGTGGGGTTGATTGATGACAGGTCAACGGCAGAAACTAGGAAAGCTTCTTCTGAACAACCAAGCCCTGTCTCTGTTCTTGATTCGACATTCTATCGCGATGACTCACCATCTCCAGTGAAGAAGATATCAAATGCCTTCAAAGGTATAGTTCTTCTGCAACTGATCCCTTATATGTGGTATAGAATGCAAGACTCGTTTATACTAAATTTCCTCAAATTTCTTGCTGCAGACGATGAAGCACAAATTCTGGATATAGCAGGGTATGACCCAATGAACCAAGCTCTGTTATCCAACAACACAATGCCCGGTCCTGGGAATCATCGATGCATGAGCAGGACACACGAGAACCCCATCACGGGTCCCCTCTGTGACAGTACAAATCCAGACCACATGTACATTTCAGAAATATTGCTGGCATCGGGTATTCTGGAATATCTCGAATCTCCCTGGACAACCACTGAGCTCCACACATCAGACCACCTGATCGACCCTACTTTGTTCCTTGCACTGGAAGATATCAGAGCAAATACAATACCTTCTGATCTCCAGCCCAAACCTGATGAGAAACTTCAAAGAAAACTAGTATTTGAGGTTGTTAATGAATTCCTTGTTCAAAAACTAGTGGTGGAAGATTCTTTGAAGCAGTGGTTCCCACCACACAAGCTGGCCGATGGGAAACCAAGAAGTCAGCAGCTTCTGAAAGAAATGTGTTCAGAGGTTGATCAGCTACAAAGAAAGAACTTGAATGGCAGCCTGGATGACGAGGATGAAGGTTTGAGAAACATCTTGTTGGAAGATTTCACGGATCAAGCAAAGAATTGGACAGACGATTGA

Protein Analysis

962

Amino Acids

107.77

Weight (kDa)

9.5

Isoelectric Point (pI)

55.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 813 - 960 3.4e-18 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1371
Acc16I TGCGCA 1 cut(s) 1872
Acc36I ACCTGC 2 cut(s) 334, 717
AccII CGCG 3 cut(s) 496, 549, 2148
AccIII TCCGGA 1 cut(s) 899
AciI CCGC 1 cut(s) 1022
AclI AACGTT 1 cut(s) 613
AclWI GGATC 9 cut(s) 675, 780, 839, 1167, 1481, 1559, 2012, 2209, 2865
AcoI YGGCCR 2 cut(s) 1341, 2718
AcsI RAATTY 8 cut(s) 39, 982, 1243, 1768, 2257, 2267, 2295, 2654
AcuI CTGAAG 2 cut(s) 916, 1083
AfaI GTAC 6 cut(s) 296, 362, 504, 955, 2428, 2447
AfeI AGCGCT 1 cut(s) 730
AfiI CCNNNNNNNGG 9 cut(s) 405, 624, 2053, 2054, 2363, 2364, 2407, 2408, 2560
AflIII ACRYGT 3 cut(s) 494, 1455, 2442
AhlI ACTAGT 3 cut(s) 1481, 2632, 2671
AjnI CCWGG 7 cut(s) 439, 721, 1489, 1822, 2362, 2498, 2805
AjuI GAANNNNNNNTTGG 2 cut(s) 914, 946
Alw21I GWGCWC 2 cut(s) 1171, 2517
Alw26I GTCTC 3 cut(s) 138, 1749, 2125
AlwI GGATC 9 cut(s) 675, 780, 839, 1167, 1481, 1559, 2012, 2209, 2865
AlwNI CAGNNNCTG 2 cut(s) 1334, 2749
Ama87I CYCGRG 2 cut(s) 207, 373
Aor13HI TCCGGA 1 cut(s) 899
Aor51HI AGCGCT 1 cut(s) 730
AoxI GGCC 3 cut(s) 377, 1341, 2718
ApeKI GCWGC 7 cut(s) 152, 329, 712, 1018, 2276, 2743, 2802
ApoI RAATTY 8 cut(s) 39, 982, 1243, 1768, 2257, 2267, 2295, 2654
ArsI GACNNNNNNTTYG 2 cut(s) 1837, 1869
AseI ATTAAT 1 cut(s) 819
Asp700I GAANNNNTTC 3 cut(s) 39, 187, 929
AspLEI GCGC 2 cut(s) 731, 1873
AspS9I GGNCC 3 cut(s) 211, 2360, 2411
AsuC2I CCSGG 1 cut(s) 2358
AsuHPI GGTGA 7 cut(s) 67, 375, 930, 1121, 1368, 1982, 2148
AsuII TTCGAA 1 cut(s) 43
AsuNHI GCTAGC 2 cut(s) 1126, 1886
AvaI CYCGRG 2 cut(s) 207, 373
AvaII GGWCC 3 cut(s) 211, 2360, 2411
BanII GRGCYC 3 cut(s) 1171, 1706, 2517
BauI CACGAG 2 cut(s) 1699, 2393
BbsI GAAGAC 4 cut(s) 1205, 1457, 1729, 1792
Bbv12I GWGCWC 2 cut(s) 1171, 2517
BbvI GCAGC 7 cut(s) 164, 341, 724, 1030, 2263, 2755, 2814
BceAI ACGGC 3 cut(s) 97, 1356, 2094
BciT130I CCWGG 7 cut(s) 441, 723, 1491, 1824, 2364, 2500, 2807
BclI TGATCA 1 cut(s) 2773
BcnI CCSGG 1 cut(s) 2358
BcoDI GTCTC 3 cut(s) 138, 1749, 2125
BcuI ACTAGT 3 cut(s) 1481, 2632, 2671
BfaI CTAG 7 cut(s) 1127, 1482, 1797, 1887, 2088, 2633, 2672
BfmI CTRYAG 4 cut(s) 327, 671, 710, 2277
BfoI RGCGCY 1 cut(s) 732
BfuAI ACCTGC 2 cut(s) 334, 717
BglII AGATCT 3 cut(s) 274, 839, 1132
BisI GCNGC 8 cut(s) 153, 330, 713, 1019, 1022, 2277, 2744, 2803
BlpI GCTNAGC 1 cut(s) 156
BlsI GCNGC 8 cut(s) 154, 331, 714, 1020, 1023, 2278, 2745, 2804
Bme1390I CCNGG 8 cut(s) 441, 723, 1491, 1824, 2358, 2364, 2500, 2807
Bme18I GGWCC 3 cut(s) 211, 2360, 2411
BmeT110I CYCGRG 2 cut(s) 207, 373
BmgT120I GGNCC 3 cut(s) 211, 2360, 2411
BmiI GGNNCC 5 cut(s) 212, 1351, 2412, 2413, 2702
BmrFI CCNGG 8 cut(s) 441, 723, 1491, 1824, 2358, 2364, 2500, 2807
BmrI ACTGGG 1 cut(s) 1845
BmtI GCTAGC 2 cut(s) 1130, 1890
BmuI ACTGGG 1 cut(s) 1845
BpiI GAAGAC 4 cut(s) 1205, 1457, 1729, 1792
BpmI CTGGAG 3 cut(s) 838, 2148, 2582
Bpu1102I GCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 43
BpuEI CTTGAG 2 cut(s) 1157, 1663
BpuMI CCSGG 1 cut(s) 2358
Bsa29I ATCGAT 2 cut(s) 1230, 2374
BsaWI WCCGGW 1 cut(s) 899
BsaXI ACNNNNNCTCC 8 cut(s) 353, 383, 616, 646, 1066, 1096, 1727, 1757
Bsc4I CCNNNNNNNGG 9 cut(s) 405, 624, 2053, 2054, 2363, 2364, 2407, 2408, 2560
Bse118I RCCGGY 1 cut(s) 252
Bse1I ACTGG 5 cut(s) 774, 829, 1851, 2165, 2565
BseAI TCCGGA 1 cut(s) 899
BseBI CCWGG 7 cut(s) 441, 723, 1491, 1824, 2364, 2500, 2807
BseCI ATCGAT 2 cut(s) 1230, 2374
BseGI GGATG 2 cut(s) 2815, 2824
BseLI CCNNNNNNNGG 9 cut(s) 405, 624, 2053, 2054, 2363, 2364, 2407, 2408, 2560
BseMII CTCAG 5 cut(s) 147, 589, 1177, 1868, 2502
BseNI ACTGG 5 cut(s) 774, 829, 1851, 2165, 2565
BseRI GAGGAG 2 cut(s) 961, 1737
BseXI GCAGC 7 cut(s) 164, 341, 724, 1030, 2263, 2755, 2814
BseYI CCCAGC 1 cut(s) 2047
BsgI GTGCAG 1 cut(s) 1611
Bsh1236I CGCG 3 cut(s) 496, 549, 2148
BshFI GGCC 3 cut(s) 379, 1343, 2720
BshVI ATCGAT 2 cut(s) 1230, 2374
BsiHKAI GWGCWC 2 cut(s) 1171, 2517
BsiHKCI CYCGRG 2 cut(s) 207, 373
BsiSI CCGG 3 cut(s) 253, 900, 2358
BslFI GGGAC 3 cut(s) 224, 780, 2397
BslI CCNNNNNNNGG 9 cut(s) 405, 624, 2053, 2054, 2363, 2364, 2407, 2408, 2560
BsmAI GTCTC 3 cut(s) 138, 1749, 2125
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 3 cut(s) 224, 780, 2397
BsmI GAATGC 2 cut(s) 922, 2241
BsnI GGCC 3 cut(s) 379, 1343, 2720
BsoBI CYCGRG 2 cut(s) 207, 373
Bsp119I TTCGAA 1 cut(s) 43
Bsp1286I GDGCHC 3 cut(s) 1171, 1706, 2517
Bsp13I TCCGGA 1 cut(s) 899
Bsp1407I TGTACA 1 cut(s) 2445
Bsp1720I GCTNAGC 1 cut(s) 156
Bsp19I CCATGG 1 cut(s) 1434
Bsp68I TCGCGA 1 cut(s) 2148
BspACI CCGC 1 cut(s) 1022
BspANI GGCC 3 cut(s) 379, 1343, 2720
BspCNI CTCAG 5 cut(s) 148, 588, 1176, 1869, 2503
BspDI ATCGAT 2 cut(s) 1230, 2374
BspEI TCCGGA 1 cut(s) 899
BspFNI CGCG 3 cut(s) 496, 549, 2148
BspHI TCATGA 1 cut(s) 966
BspLI GGNNCC 5 cut(s) 212, 1351, 2412, 2413, 2702
BspMAI CTGCAG 4 cut(s) 331, 675, 714, 2281
BspMI ACCTGC 2 cut(s) 334, 717
BspOI GCTAGC 2 cut(s) 1130, 1890
BspPI GGATC 9 cut(s) 675, 780, 839, 1167, 1481, 1559, 2012, 2209, 2865
BspT104I TTCGAA 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 252
BsrGI TGTACA 1 cut(s) 2445
BsrI ACTGG 5 cut(s) 774, 829, 1851, 2165, 2565
BssAI RCCGGY 1 cut(s) 252
BssSI CACGAG 2 cut(s) 1699, 2393
BssT1I CCWWGG 7 cut(s) 404, 461, 587, 1010, 1344, 1434, 1544
Bst2BI CACGAG 2 cut(s) 1699, 2393
Bst2UI CCWGG 7 cut(s) 441, 723, 1491, 1824, 2364, 2500, 2807
Bst4CI ACNGT 6 cut(s) 100, 488, 568, 741, 1291, 2426
Bst6I CTCTTC 2 cut(s) 471, 532
BstAUI TGTACA 1 cut(s) 2445
BstBI TTCGAA 1 cut(s) 43
BstC8I GCNNGC 9 cut(s) 254, 494, 1128, 1300, 1757, 1888, 2015, 2466, 2718
BstDEI CTNAG 6 cut(s) 156, 575, 987, 1163, 1877, 2511
BstDSI CCRYGG 3 cut(s) 307, 1338, 1434
BstF5I GGATG 2 cut(s) 2815, 2824
BstFNI CGCG 3 cut(s) 496, 549, 2148
BstH2I RGCGCY 1 cut(s) 732
BstHHI GCGC 2 cut(s) 731, 1873
BstMAI GTCTC 3 cut(s) 138, 1749, 2125
BstMWI GCNNNNNNNGC 5 cut(s) 350, 709, 1269, 1953, 2023
BstNI CCWGG 7 cut(s) 441, 723, 1491, 1824, 2364, 2500, 2807
BstNSI RCATGY 1 cut(s) 2446
BstSCI CCNGG 8 cut(s) 439, 721, 1489, 1822, 2356, 2362, 2498, 2805
BstSFI CTRYAG 4 cut(s) 327, 671, 710, 2277
BstUI CGCG 3 cut(s) 496, 549, 2148
BstV1I GCAGC 7 cut(s) 164, 341, 724, 1030, 2263, 2755, 2814
BstV2I GAAGAC 4 cut(s) 1205, 1457, 1729, 1792
BstX2I RGATCY 8 cut(s) 274, 667, 772, 839, 1132, 1159, 1564, 2004
BstYI RGATCY 8 cut(s) 274, 667, 772, 839, 1132, 1159, 1564, 2004
Bsu15I ATCGAT 2 cut(s) 1230, 2374
BsuRI GGCC 3 cut(s) 379, 1343, 2720
BsuTUI ATCGAT 2 cut(s) 1230, 2374
BtgI CCRYGG 3 cut(s) 307, 1338, 1434
BtgZI GCGATG 4 cut(s) 564, 1205, 1629, 2163
BtsCI GGATG 2 cut(s) 2815, 2824
BtsI GCAGTG 2 cut(s) 2058, 2702
BtsIMutI CAGTG 7 cut(s) 96, 873, 2058, 2172, 2508, 2558, 2702
BtuMI TCGCGA 1 cut(s) 2148
BveI ACCTGC 2 cut(s) 334, 717
Cac8I GCNNGC 9 cut(s) 254, 494, 1128, 1300, 1757, 1888, 2015, 2466, 2718
CaiI CAGNNNCTG 2 cut(s) 1334, 2749
CciI TCATGA 1 cut(s) 966
CfoI GCGC 2 cut(s) 731, 1873
Cfr10I RCCGGY 1 cut(s) 252
Cfr13I GGNCC 3 cut(s) 211, 2360, 2411
ClaI ATCGAT 2 cut(s) 1230, 2374
Csp6I GTAC 6 cut(s) 295, 361, 503, 954, 2427, 2446
CviQI GTAC 6 cut(s) 295, 361, 503, 954, 2427, 2446
DdeI CTNAG 6 cut(s) 156, 575, 987, 1163, 1877, 2511
DraI TTTAAA 1 cut(s) 1144
EaeI YGGCCR 2 cut(s) 1341, 2718
Eam1104I CTCTTC 2 cut(s) 471, 532
EarI CTCTTC 2 cut(s) 471, 532
Ecl136II GAGCTC 2 cut(s) 1169, 2515
Eco130I CCWWGG 7 cut(s) 404, 461, 587, 1010, 1344, 1434, 1544
Eco147I AGGCCT 1 cut(s) 379
Eco24I GRGCYC 3 cut(s) 1171, 1706, 2517
Eco32I GATATC 2 cut(s) 2178, 2569
Eco47I GGWCC 3 cut(s) 211, 2360, 2411
Eco47III AGCGCT 1 cut(s) 730
Eco53kI GAGCTC 2 cut(s) 1169, 2515
Eco57I CTGAAG 2 cut(s) 916, 1083
Eco88I CYCGRG 2 cut(s) 207, 373
EcoICRI GAGCTC 2 cut(s) 1169, 2515
EcoO109I RGGNCCY 2 cut(s) 211, 2411
EcoRI GAATTC 3 cut(s) 982, 1768, 2654
EcoRII CCWGG 7 cut(s) 439, 721, 1489, 1822, 2362, 2498, 2805
EcoRV GATATC 2 cut(s) 2178, 2569
EcoT14I CCWWGG 7 cut(s) 404, 461, 587, 1010, 1344, 1434, 1544
EcoT22I ATGCAT 3 cut(s) 355, 556, 2381
EcoT38I GRGCYC 3 cut(s) 1171, 1706, 2517
ErhI CCWWGG 7 cut(s) 404, 461, 587, 1010, 1344, 1434, 1544
Esp3I CGTCTC 1 cut(s) 138
FalI AAGNNNNNCTT 4 cut(s) 580, 612, 914, 946
FaqI GGGAC 3 cut(s) 224, 780, 2397
FbaI TGATCA 1 cut(s) 2773
Fnu4HI GCNGC 8 cut(s) 153, 330, 713, 1019, 1022, 2277, 2744, 2803
FokI GGATG 2 cut(s) 2822, 2831
FriOI GRGCYC 3 cut(s) 1171, 1706, 2517
Fsp4HI GCNGC 8 cut(s) 153, 330, 713, 1019, 1022, 2277, 2744, 2803
FspBI CTAG 7 cut(s) 1127, 1482, 1797, 1887, 2088, 2633, 2672
FspI TGCGCA 1 cut(s) 1872
GlaI GCGC 2 cut(s) 730, 1872
GluI GCNGC 8 cut(s) 153, 330, 713, 1019, 1022, 2277, 2744, 2803
GsaI CCCAGC 1 cut(s) 2051
GsuI CTGGAG 3 cut(s) 838, 2148, 2582
HaeII RGCGCY 1 cut(s) 732
HaeIII GGCC 3 cut(s) 379, 1343, 2720
HapII CCGG 3 cut(s) 253, 900, 2358
HhaI GCGC 2 cut(s) 731, 1873
Hin6I GCGC 2 cut(s) 729, 1871
HinP1I GCGC 2 cut(s) 729, 1871
HincII GTYRAC 3 cut(s) 792, 1516, 2076
HindII GTYRAC 3 cut(s) 792, 1516, 2076
HindIII AAGCTT 3 cut(s) 446, 1472, 2094
HpaII CCGG 3 cut(s) 253, 900, 2358
HphI GGTGA 7 cut(s) 67, 375, 930, 1121, 1368, 1982, 2148
Hpy166II GTNNAC 4 cut(s) 792, 1516, 1990, 2076
Hpy8I GTNNAC 4 cut(s) 792, 1516, 1990, 2076
HpyAV CCTTC 7 cut(s) 390, 475, 859, 1592, 2197, 2598, 2816
HpyCH4III ACNGT 6 cut(s) 100, 488, 568, 741, 1291, 2426
HpyCH4IV ACGT 5 cut(s) 293, 359, 613, 1457, 1608
HpyF10VI GCNNNNNNNGC 5 cut(s) 350, 709, 1269, 1953, 2023
HpyF3I CTNAG 6 cut(s) 156, 575, 987, 1163, 1877, 2511
HpySE526I ACGT 5 cut(s) 293, 359, 613, 1457, 1608
HspAI GCGC 2 cut(s) 729, 1871
KflI GGGWCCC 1 cut(s) 2411
Kpn2I TCCGGA 1 cut(s) 899
KroI GCCGGC 1 cut(s) 252
KroNI GCCGGC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 2773
LmnI GCTCC 4 cut(s) 341, 1012, 1087, 2520
Lsp1109I GCAGC 7 cut(s) 164, 341, 724, 1030, 2263, 2755, 2814
MaeI CTAG 7 cut(s) 1127, 1482, 1797, 1887, 2088, 2633, 2672
MaeII ACGT 5 cut(s) 293, 359, 613, 1457, 1608
MaeIII GTNAC 4 cut(s) 543, 632, 1496, 2420
MflI RGATCY 8 cut(s) 274, 667, 772, 839, 1132, 1159, 1564, 2004
MhlI GDGCHC 3 cut(s) 1171, 1706, 2517
MluI ACGCGT 1 cut(s) 494
MlyI GAGTC 7 cut(s) 281, 362, 434, 551, 1918, 2147, 2237
MmeI TCCRAC 3 cut(s) 216, 2367, 2823
Mph1103I ATGCAT 3 cut(s) 355, 556, 2381
MroI TCCGGA 1 cut(s) 899
MroNI GCCGGC 1 cut(s) 252
MroXI GAANNNNTTC 3 cut(s) 39, 187, 929
MseI TTAA 8 cut(s) 321, 420, 809, 819, 1143, 1173, 1241, 2649
MslI CAYNNNNRTG 2 cut(s) 1895, 1917
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 3 cut(s) 253, 900, 2358
MspR9I CCNGG 8 cut(s) 441, 723, 1491, 1824, 2358, 2364, 2500, 2807
Mva1269I GAATGC 2 cut(s) 922, 2241
MvaI CCWGG 7 cut(s) 441, 723, 1491, 1824, 2364, 2500, 2807
MvnI CGCG 3 cut(s) 496, 549, 2148
MwoI GCNNNNNNNGC 5 cut(s) 350, 709, 1269, 1953, 2023
NaeI GCCGGC 1 cut(s) 254
NciI CCSGG 1 cut(s) 2358
NcoI CCATGG 1 cut(s) 1434
NgoMIV GCCGGC 1 cut(s) 252
NheI GCTAGC 2 cut(s) 1126, 1886
NlaIV GGNNCC 5 cut(s) 212, 1351, 2412, 2413, 2702
NmuCI GTSAC 2 cut(s) 543, 2420
NruI TCGCGA 1 cut(s) 2148
NsbI TGCGCA 1 cut(s) 1872
NsiI ATGCAT 3 cut(s) 355, 556, 2381
NspI RCATGY 1 cut(s) 2446
NspV TTCGAA 1 cut(s) 43
PagI TCATGA 1 cut(s) 966
PceI AGGCCT 1 cut(s) 379
PciI ACATGT 1 cut(s) 2442
PctI GAATGC 2 cut(s) 922, 2241
PdiI GCCGGC 1 cut(s) 254
PdmI GAANNNNTTC 3 cut(s) 39, 187, 929
PfeI GAWTC 9 cut(s) 83, 782, 959, 1442, 1922, 2132, 2368, 2492, 2684
PfoI TCCNGGA 1 cut(s) 439
PkrI GCNGC 8 cut(s) 154, 331, 714, 1020, 1023, 2278, 2745, 2804
PleI GAGTC 7 cut(s) 281, 362, 433, 550, 1917, 2147, 2237
PpsI GAGTC 7 cut(s) 281, 362, 433, 550, 1917, 2147, 2237
PpuMI RGGWCCY 2 cut(s) 211, 2411
PscI ACATGT 1 cut(s) 2442
PshBI ATTAAT 1 cut(s) 819
PsiI TTATAA 1 cut(s) 1371
Psp124BI GAGCTC 2 cut(s) 1171, 2517
Psp1406I AACGTT 1 cut(s) 613
Psp5II RGGWCCY 2 cut(s) 211, 2411
Psp6I CCWGG 7 cut(s) 439, 721, 1489, 1822, 2362, 2498, 2805
PspFI CCCAGC 1 cut(s) 2047
PspGI CCWGG 7 cut(s) 439, 721, 1489, 1822, 2362, 2498, 2805
PspN4I GGNNCC 5 cut(s) 212, 1351, 2412, 2413, 2702
PspPI GGNCC 3 cut(s) 211, 2360, 2411
PspPPI RGGWCCY 2 cut(s) 211, 2411
PstI CTGCAG 4 cut(s) 331, 675, 714, 2281
PstNI CAGNNNCTG 2 cut(s) 1334, 2749
PsuI RGATCY 8 cut(s) 274, 667, 772, 839, 1132, 1159, 1564, 2004
PvuII CAGCTG 1 cut(s) 155
RruI TCGCGA 1 cut(s) 2148
RsaI GTAC 6 cut(s) 296, 362, 504, 955, 2428, 2447
RsaNI GTAC 6 cut(s) 295, 361, 503, 954, 2427, 2446
RseI CAYNNNNRTG 2 cut(s) 1895, 1917
SacI GAGCTC 2 cut(s) 1171, 2517
SaqAI TTAA 8 cut(s) 321, 420, 809, 819, 1143, 1173, 1241, 2649
SatI GCNGC 8 cut(s) 153, 330, 713, 1019, 1022, 2277, 2744, 2803
Sau96I GGNCC 3 cut(s) 211, 2360, 2411
SchI GAGTC 7 cut(s) 281, 362, 434, 551, 1918, 2147, 2237
ScrFI CCNGG 8 cut(s) 441, 723, 1491, 1824, 2358, 2364, 2500, 2807
SduI GDGCHC 3 cut(s) 1171, 1706, 2517
SfcI CTRYAG 4 cut(s) 327, 671, 710, 2277
SfuI TTCGAA 1 cut(s) 43
SinI GGWCC 3 cut(s) 211, 2360, 2411
SmiMI CAYNNNNRTG 2 cut(s) 1895, 1917
SmlI CTYRAG 2 cut(s) 1136, 1678
SmoI CTYRAG 2 cut(s) 1136, 1678
SpeI ACTAGT 3 cut(s) 1481, 2632, 2671
SseBI AGGCCT 1 cut(s) 379
SsiI CCGC 1 cut(s) 1022
SspI AATATT 1 cut(s) 2460
SspMI CTAG 7 cut(s) 1127, 1482, 1797, 1887, 2088, 2633, 2672
SstI GAGCTC 2 cut(s) 1171, 2517
StuI AGGCCT 1 cut(s) 379
StyD4I CCNGG 8 cut(s) 439, 721, 1489, 1822, 2356, 2362, 2498, 2805
StyI CCWWGG 7 cut(s) 404, 461, 587, 1010, 1344, 1434, 1544
TaaI ACNGT 6 cut(s) 100, 488, 568, 741, 1291, 2426
TaiI ACGT 5 cut(s) 296, 362, 616, 1460, 1611
TaqI TCGA 8 cut(s) 43, 980, 1230, 1576, 2135, 2374, 2490, 2538
TatI WGTACW 2 cut(s) 2426, 2445
TauI GCSGC 1 cut(s) 1024
TfiI GAWTC 9 cut(s) 83, 782, 959, 1442, 1922, 2132, 2368, 2492, 2684
Tru1I TTAA 8 cut(s) 321, 420, 809, 819, 1143, 1173, 1241, 2649
Tru9I TTAA 8 cut(s) 321, 420, 809, 819, 1143, 1173, 1241, 2649
TscAI CASTG 7 cut(s) 103, 880, 2058, 2172, 2515, 2565, 2702
TseFI GTSAC 2 cut(s) 543, 2420
TseI GCWGC 7 cut(s) 152, 329, 712, 1018, 2276, 2743, 2802
Tsp45I GTSAC 2 cut(s) 543, 2420
TspGWI ACGGA 2 cut(s) 775, 2870
TspRI CASTG 7 cut(s) 103, 880, 2058, 2172, 2515, 2565, 2702
VpaK11BI GGWCC 3 cut(s) 211, 2360, 2411
VspI ATTAAT 1 cut(s) 819
XapI RAATTY 8 cut(s) 39, 982, 1243, 1768, 2257, 2267, 2295, 2654
XceI RCATGY 1 cut(s) 2446
XmnI GAANNNNTTC 3 cut(s) 39, 187, 929
XspI CTAG 7 cut(s) 1127, 1482, 1797, 1887, 2088, 2633, 2672
Zsp2I ATGCAT 3 cut(s) 355, 556, 2381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.